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isolation and characterization of fish scale collagen of higher thermal stability.collagen is the most abundant protein found in animal body and widely used for biomedical and pharmaceutical applications. however, its applicability is severely limited due to high cost. fish processing waste, which otherwise cause serious environmental pollution, is a promising cost effective collagen source. in the present study, collagen was isolated from scales of labeo rohita (rohu) and catla catla (catla). it is first time that these species are used as sources of collagen. thermo-gravime ...201020116238
behavioral and morphological endpoints: as an early response to sublethal malathion intoxication in the freshwater fish, labeo rohita.a short-term definitive test by the static renewal bioassay method was conducted to determine the acute toxicity (lc(50)) of commercial-grade organophosphate insecticide, malathion (50% ec) on the freshwater fish, labeo rohita. carp fingerlings were exposed to different concentrations of malathion for 96 hours. the acute toxicity (lc(50)) of malathion was found to be 4.5 microg/l. one tenth (1/10, 0.45 microg/l) of the acute toxicity value was selected as the sublethal concentration for subacute ...201020307142
kinetic study of hepatic β-glucuronidase in the indian major carp, labeo rohita (hamilton).liver is the metabolic factory and contains several valuable enzymes that catalyze biochemical reactions. β-glucuronidase is one of the well-known lysozymes that participates in the carbohydrate metabolism in the tissues of various vertebrates. in the present study, an attempt was made to study the kinetic properties of hepatic β-glucuronidase of the indian major carp (imc), labeo rohita. it was observed that the enzyme activity increased largely at ph 5 (0.1 m acetate buffer) when exposed to 38 ...201020336485
inhibitory effect of pistia tannin on digestive enzymes of indian major carps: an in vitro study.aquatic weeds are one of the major unconventional feed ingredients tested for aquafeed formulation. tannin content in the water lettuce, pistia, has been quantified (26.67 mg g(-1); dry weight) and graded levels of which (12.5-200 μg) have been incorporated in the reaction mixtures to evaluate any change in the in vitro activity of the principal digestive enzymes from the three indian major carps (imc), namely rohu (labeo rohita), catla (catla catla) and mrigala (cirrhinus mrigala). result of th ...201020369287
fish-borne zoonotic trematodes in cultured and wild-caught freshwater fish from the red river delta, vietnam.nam dinh province in red river delta, vietnam, is an endemic area for the human liver fluke clonorchis sinensis, but little is known about its occurrence in the fish intermediate host in this region. a cross-sectional study was carried out to identify fish-borne zoonotic trematodes (fzt) species diversity and to estimate the prevalence and infection densities of fzt in three major types of fish: cultured species and self-recruiting species from family ponds and wild-caught species from irrigatio ...201020370427
assessing metal, protein, and dna profiles in labeo rohita from the indus river in mianwali, pakistan.this study assessed metals in water and different tissues of labeo rohita and the impact of these metals on dna and proteins as biomarkers of gills and muscles of these fish from three different polluted sites (reference or low = kw, medium = ch and high = sk) of the indus river, pakistan. the mn, pb, cu, zn, hg, and cr levels in water, gills, liver, muscles, and skin of these fish were compared with the international permissible levels. all metals except pb and hg in water were within the accep ...201120461548
effect of withania somnifera (l. dunal) root as a feed additive on immunological parameters and disease resistance to aeromonas hydrophila in labeo rohita (hamilton) fingerlings.the present study evaluated the efficacy of dietary doses of withania somnifera (ashwagandha) root powder on immunological parameters and disease resistance against the bacterial pathogen aeromonas hydrophila infections in indian major carp, labeo rohita fingerlings. fishes were fed with dry diet containing 0 gkg(-1) (control), 1 gkg(-1) (t(1)), 2 gkg(-1) (t(2)) and 3 gkg(-1) (t(3)) w. somnifera root powder for 42 days. immunological (nbt level, phagocytic activity, total immunoglobulin and lyso ...201020580830
development and characterization of three new diploid cell lines from labeo rohita (ham.).development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. three new diploid fish cell lines (rf, rh, and rsb) were developed from fin, heart, and swim bladder of an indian major carp, labeo rohita, commonly called rohu. all the cell lines were optimally maintained at 28 degrees c in leibovitz-15 medium supplemented with 10% fbs. the propagation of rh ...201020730759
biochemical and stress responses of rohu labeo rohita and mrigal cirrhinus mrigala in relation to acclimation temperatures.the biochemical and stress responses of two indian major carps, rohu labeo rohita and mrigal cirrhinus mrigala were studied after acclimating them to four preset temperatures (26, 31, 33 and 36 degrees c) for 30 days. the blood glucose and liver glycogen levels showed an inverse trend in both the species and were significantly different in l. rohita at higher temperatures. the decrease in the liver glycogen level of c. mrigala, however, was not significant. plasma cortisol levels increased signi ...200920735648
haemato-immunology and histo-architectural changes in labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor.a feeding trail of 60 days was conducted for delineating the effect of dietary aflatoxin (afb1) with or without supplementation of a mixture of mould inhibitor (0.25% clove oil + 0.32% sodium propionate) on haematology, respiratory burst activity and histology of labeo rohita fingerlings. three hundred and sixty fishes (avg. wt. 1.48-1.54 g) were randomly distributed into eight treatment groups. eight experimental diets with four different levels of aflatoxin (0, 10, 20 and 40 ppb) with or witho ...201120803068
yeast extract, brewer's yeast and spirulina in diets for labeo rohita fingerlings affect haemato-immunological responses and survival following aeromonas hydrophila challenge.a feeding trial was conducted for 60days to study the immunomodulatory role of three different immunostimulants yeast extract (ye), brewer's yeast (by) and spirulina (sp) in labeo rohita fingerlings. four hundred and fifty fingerlings (avg. wt 3.35±0.15g) were randomly distributed in ten treatments and fed with either of ten iso-nitrogenous and iso-caloric semi-purified diets, prepared with three incremental levels (1%, 2% and 4%) of different immunostimulants except the control. growth paramete ...201020825959
studies on lipid profiles and fatty acid composition of roe from rohu (labeo rohita) and murrel (channa striatus).the lipid profiles and fatty acid composition of roes from two common fresh water fish viz., rohu (labeo rohita) and murrel (channa striatus) have been studied. the dehydrated fish egg was ground to fine powder and extracted with chloroform/methanol (2:1, v/v) to recover the total lipid in 20.2 and 22.7% on dry weight basis. the fat free meal was found to contain 70 and 58% protein in rohu and murrel, respectively. the total lipids were separated into neutral lipids, glycolipids and phospholipid ...201020877143
simple gas chromatographic method for the quantification of total cholesterol in fish meats.six fish species-cirrhinus mrigala (morakhi), labeo rohita (danbhro), catla catla (thalli), wallago attu (jarko), ctenopharyngodon idella (grass), and cyprimus carpio (gulfam)--commonly found in the pakistani indus river, were selected for fat and cholesterol evaluation. fat content was quantified by the folch method and cholesterol content was determined by a simple gc method. the application of the proposed method to quantify cholesterol content revealed variations among the six breeds evaluat ...201020922959
expression of immune-related genes in rohu labeo rohita (hamilton) by experimental freshwater lice argulus siamensis (wilson) infection.the crustacean ectoparasite, argulus poses one of the major threats to carp culture due to absence of any suitable control measure. the study was undertaken to determine the expression of immune-related genes in three major immunocompetent organs viz., kidney, skin and liver of rohu (labeo rohita) during experimental freshwater lice argulus siamensis infection. results showed that the expression of tlr 22-like, lysozyme g and β2-microglobulin genes in kidney was significantly (p ≤ 0.05) down-reg ...201021051150
application of calcined waste fish (labeo rohita) scale as low-cost heterogeneous catalyst for biodiesel synthesis.this paper explores the feasibility of converting waste rohu fish (labeo rohita) scale into a high-performance, reusable, low-cost heterogeneous catalyst for synthesis of biodiesel from soybean oil. the thermo-gravimetric analysis (tga) and x-ray diffraction (xrd) analysis revealed that a significant portion of the main component of fish scale i.e. hap (hydroxyapatite) could be transformed into β-tri-calcium phosphate when calcined above 900°c for 2 h. scanning electron microscopy (sem) morpholo ...201021094040
higher water temperature enhances dietary carbohydrate utilization and growth performance in labeo rohita (hamilton) fingerlings.a 60-day experiment was conducted to delineate the effect of three dietary levels of gelatinized carbohydrate (gc) on growth, nutrient-utilization and body composition of labeo rohita fingerlings (avg. wt 6.5 ± 0.3 g) reared at two water temperatures (ambient-at (26 ± 0.8 °c) and 32 °c). two hundred and sixteen fingerlings were randomly distributed into six treatments in triplicates. three semi-purified isonitrogenous diets were prepared with graded levels of gc viz. d(1) : 40%, d(2) : 50% and d ...201021114547
haemato-immunological and stress responses of labeo rohita (hamilton) fingerlings: effect of rearing temperature and dietary gelatinized carbohydrate.a feeding trial of 70-days was carried out to study the haemato-immunological and stress responses of labeo rohita fingerlings reared at two water temperatures [ambient (amb) - 27 °c and 32 °c] fed with graded levels of gelatinized corn carbohydrate (gc). two hundred and sixteen fingerlings were randomly distributed into six treatment groups in triplicate. three semi-purified diets were prepared containing 30% crude protein with graded levels of gc 40%, 50% and 58%. the six treatment groups were ...201021114548
the ontogeny of mauthner cells in the brain of labeo rohita as revealed by nadph-d and nnos immunohistochemistry.nitric oxide (no) is well demonstrated to act as a neuronal messenger in neurotransmission in vertebrate animals. we are for the first time reporting nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) and neuronal nitric oxide synthase (nnos) in the mauthner cells (mc) of the carp, labeo rohita brain during post-embryonic development. the nadph-d and nnos-positive mc were seen intensely labeled in all young stages on either side of the ventricle in the hindbrain. in adult, these ce ...201021132510
application of comet assay in the study of dna damage and recovery in rohu (labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide.labeo rohita (rohu) fingerlings were exposed to different concentrations (0.001, 0.002 and 0.01 ppm) of phorate, an organophosphate pesticide; samplings were done at 24, 48, 72 and 96 h. the study was carried out to evaluate tissue specific genotoxic effects produced by phorate, on three different tissue systems and to assess dna repair response in fish. results of tissue specific dna damage experiments showed low baseline damage in blood cells followed by gill and liver cells in control individ ...201021153054
monoclonal antibodies to snakehead, channa striata immunoglobulins: detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs.snakehead channa striata is an important freshwater food fish in many southeast asian countries. three monoclonal antibodies (c9, c10 and d10) were developed against purified serum immunoglobulins of channa striata (cs-ig) and characterized. c9 and d10 mabs were specific to heavy chain, while c10 mab detected only unreduced cs-ig in western blotting. in competitive elisa, c9 and c10 mabs were specific to c. striata ig and showed no cross reactivity with serum ig of other fish species i.e. channa ...201021167285
effect of surfactants on phosphatase level of fresh water fish labeo rohita.alterations in the activity of enzymes acid phosphatase (e.c.3.1.3.2) and alkaline phosphatase (ec 3.1.3.1) in organs such as liver, gills and muscle of rohu following its exposure to surfactants viz. ctab, sds and triton x-100 were analyzed. different levels of exposure were given depending on the lc50 value of the surfactant used. also, the influence of age and weight of the organisms was tested simultaneously. the activity of acp in the tissues of fish exposed to all the three surfactants sho ...201021186708
indian carp (labeo rohita) gall bladder poisoning-report of four cases in a single family.the ingestion of indian carp gallbladder may result in transient hepatitis with subsequent acute renal failure. this case series also illustrates the importance of understanding the use and potential serious complications of alternative medicines. so fish gallbladder poisoning should be considered in unexplained acute renal failure in chinese and asian patients. we report four family members who developed acute renal failure and toxic hepatitis at the same time following ingestion of raw indian ...201121207198
comparative susceptibility of carp fingerlings to lernaea cyprinacea infection.study was conducted to find out the comparative susceptibility of fingerlings of seven species of carps (labeo fimbriatus, l. rohita, l. calbasu, catla catla, ctenopharyngodon idella, cyprinus carpio and hypophthamichthys molitrix) grown under both mono and polyculture to lernaea cyprinacea infection. under monoculture, c. carpio, l. rohita and l. calbasu, did not acquire lernaea infection and were thus considered resistant, whereas c. idella, h. molitrix, c. catla and l. fimbriatus were suscept ...201021255932
ontogeny and tissue-specific expression of innate immune related genes in rohu, labeo rohita (hamilton).the innate immune response in fish represents an early and rapid defense against pathogens. the present study aims at looking into ontogeny of innate immune system in the teleost, labeo rohita using rt-pcr based approach. total rna extracted from unfertilized and fertilized eggs, and hatchlings (hatched at 28 ± 2 °c) at 0, 1, 3, 6, 12, 24 h, and 3, 7, 16, 21, 31 days post-fertilization were subjected to rt-pcr using self-designed or earlier published primers to amplify some innate immune relevan ...201121362484
application of sensory and microbial analysis to assess quality of fish in siliguri city of west bengal, india.sensory and certain microbial analyses were applied to assess the quality of raw fish sold at a market in siliguri cityof west bengal, india. in regular surveys undertaken during june to august 2008, a particular fish species was randomly selected, its source was noted and a sensory analysis, the quality index method (qim) was applied to assess its quality raw fish samples were also collected and a small quantity (about 1 g) of scales oran upper layer of the skin surface (forscale-less fish samp ...201021387907
two new species of myxobolus (myxozoa: myxosporea: bivalvulida) infecting indian freshwater fishes in punjab wetlands (india).a survey of parasites of freshwater fishes in harike and ropar wetlands of punjab (india) revealed the presence of two new myxosporean species belonging to the genus myxobolus butschli 1882. spores of the first species, m. duodenalis sp. nov. parasitize the inner wall of the duodenum of wallago attu (bloch and schneider) (cypriniformis: cyprinidae) vern. mulli are characterized by a broad, pyriform shape with a blunt anterior end and measures 9.0 +ù 3.20 ++m. spore valves are thin, smooth, and s ...201121394535
heavy metals in eight edible fish species from two polluted tributaries (aik and palkhu) of the river chenab, pakistan.concentration of heavy metals (lead (pb), cadmium (cd), chromium (cr), and copper (cu)) was determined in the liver, gills, kidneys, and muscles of eight edible fish species (channa punctata, cirrhinus reba, labeo rohita, heteropneustes fossilis, mystus cavasius, oreochromis niloticus, puntius sophore, and wallago attu) from upstream and downstream zones of the nullah aik and palkhu tributaries of the river chenab located in the sialkot district known for its tanning industries worldwide. the pa ...201121424780
isolation of enriched carp spermatogonial stem cells from labeo rohita testis for in vitro propagation.the in vitro culture system for spermatogonial stem cells (sscs) is a powerful tool for exploring molecular mechanisms of male gametogenesis and gene manipulation. very little information is available for fish ssc biology. our aim was to isolate highly pure sscs from the testis of commercially important farmed carp, labeo rohita. the minced testis of l. rohita was dissociated with collagenase. dissociated cells purified by two-step ficoll gradient centrifugation followed by magnetic activated ce ...201121496900
immunomodulating potency of lipopolysaccharides (lps) derived from smooth type of bacterial pathogens in indian major carp.lipopolysaccharides (lps), the outer membrane of the gram-negative bacteria, are reported to stimulate the immunity of different vertebrates including fish. however, their potency and spectrum of actions often differ among different bacteria. in this study, effect of crude lps, derived from three species of smooth gram-negative bacterial fish pathogens viz. edwardsiella tarda, escherichia coli, and pseudomonas fluorescens, on certain innate immune parameters of indian major carp, labeo rohita wa ...201121507588
a new myxosporean species myxobolus sclerii sp. nov. and one known species m. stomum ali et al. 2003 from two indian major carp fishes.the present communication deals with description of one new species of myxobolus (myxozoa: myxosporea: bivalvulida), m. sclerii sp. nov. infecting eye ball of catla catla (hamilton) and redescription of m. stomum infecting scales of labeo rohita (hamilton), two major carps of kanjali and ropar wetlands respectively. spores of m. sclerii sp. nov. measure 7.9-9.5(8.7 ± 1.13) × 4.3-5.7(5 ± 0.98) μm in size. parietal folds absent. polar capsules two, equal and measuring 4-5.4(4.7 ± 0.98) × 1-2.6(1.8 ...201021526031
observation on prevalence of ectoparasites in carp fingerlings in two districts of west bengal.the present study has been carried out to find out the prevalence of ectoparasites of carp fingerlings during different months of the year 2008-2009. four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from 400 fingerlings of rohu (labeo rohita), catla (catla catla), mrigal (cirrhinus mrigala), bata (labeo bata), common carp (cyprinus carpio) and lata (channa punctatus) collected from different ponds of nadia and hooghly district of west bengal from j ...201021526033
physical, mechanical, and barrier properties of carp and mammalian skin gelatin films.films of 0.11 to 0.13 mm thickness were prepared using gelatins from the skins of cultured freshwater carp species and mammalian gelatins viz., porcine and bovine skin gelatin. a comparative study was made on the physical, mechanical, and barrier properties of these films. the amino acid composition, gel strength, clarity, and gel setting point of the gelatins were also determined. carp skin gelatins had a lower imino acid content (19.16% to 20.86%) than mammalian skin gelatins (22.91% to 23.7%) ...201021535597
high dietary protein combats the stress of labeo rohita fingerlings exposed to heat shock.the amelioration effect of high dietary protein against stress was evaluated in labeo rohita fingerlings, exposed to heat shock. two hundred and forty fingerlings (6.57 ± 0.04 g, average weight ± se) were randomly distributed into 4 treatment groups, each with 4 replicates was fed with either of four diets containing different levels of protein (20, 30, 40 or 45%). water temperatures of all the treatments were within the range of 25.5-26.5°c throughout the experimental period of 30 days. after 3 ...201121573753
water soaking and exogenous enzyme treatment of plant-based diets: effect on growth performance, whole-body composition, and digestive enzyme activities of rohu, labeo rohita (hamilton), fingerlings.a 2 × 2 × 2 factorial experiment was conducted to delineate the main effect of water soaking of plant ingredients, phytase, cellulase, and their interactions on the growth and digestive enzyme activities of labeo rohita fingerlings. two basal diets were prepared using water-soaked (s) or unsoaked (us) plant-based ingredients. feed of us ingredients was supplemented with phytase (u kg(-1)) and cellulase (%) at the level of 0, 0 (c(us)); 500, 0 (t(1)); 0, 0.2 (t(2)); 500, 0.2 (t(3)), and feed of s ...201121611720
physical mapping of 5s rdna in two species of knifefishes: gymnotus pantanal and gymnotus paraguensis (gymnotiformes).physical mapping of 5s rdna in 2 species of knifefishes, gymnotuspantanal and g. paraguensis (gymnotiformes), was performed using fluorescence in situ hybridization with a 5s rdna probe. the 5s rdna pcr product from the genomes of both species was also sequenced and aligned to determine non-transcribed spacer sequences (nts). both species under study had different patterns of 5s rdna gene cluster distribution. while in the karyotype of g. pantanal two 5s rdna-bearing pairs were observed, the kar ...201121654160
effects of dietary pyridoxine on haemato-immunological responses of labeo rohita fingerlings reared at higher water temperature.a sixty-day feeding trial was carried out to elucidate the effect of dietary pyridoxine (pn) on hemato-immunological parameters in labeo rohita fingerlings exposed to an elevated temperature (et) of 33ôçâ-¦c. two hundred and seventy fingerlings (6.71ôçâ-¦ôçâ0.32ôçâg) were randomly distributed into six treatments in triplicates (15ôçâfish/tank). five iso-nitrogenous (356.3ôçâ-¦ôçâ2.7ôçâg/kg crude protein) purified diets were prepared with graded levels (0, 10, 50, 100 and 200ôçâmg/kg diet) of pn. ...201121692873
selection for improved resistance to aeromonas hydrophila in indian major carp labeo rohita: survival and innate immune responses in first generation of resistant and susceptible lines.selection for disease resistance in fish may be performed directly on basis of survival data obtained in controlled challenge trials, or indirectly using information from immunological or molecular markers linked to differential survival. in the present study, several key innate immune parameters were measured in aeromoniasis resistant and susceptible lines of rohu labeo rohita to assess their suitability as immune markers for use in indirect selection for increased resistance. experimental infe ...201121712094
methyl mercury in fish-a case study on various samples collected from ganges river at west bengal.this study investigated the presence of total mercury (hg) and organic mercury levels in the muscle of 19 common fresh water fish species captured from river ganges, west bengal, india. the total mercury level found in our study may not cause any toxic effect, but the methyl mercury (mehg) level in some freshwater fish species was surprisingly very high and toxically unacceptable. the results of mercury analysis in various specimens indicated that some fish muscles tended to accumulate high leve ...201121713467
genetic divergence in wild population of labeo rohita (hamilton, 1822) from nine indian rivers, analyzed through mtdna cytochrome b region.the present study examined partial cytochrome b gene sequence of mitochondrial dna for polymorphism and its suitability to determine the genetic differentiation in wild labeo rohita. the 146 samples of l. rohita were collected from nine distant rivers; satluj, brahmaputra, son, chambal mahanadi, rapti, chauka, bhagirathi and tons were analyzed. sequencing of 307 bp of cyto b gene revealed 35 haplotypes with haplotype diversity 0.751 and nucleotide diversity (p) 0.005. the within population varia ...201121748320
molecular characterization of nucleotide binding and oligomerization domain (nod)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu (labeo rohita).nucleotide-binding and oligomerization domain (nod)-2 is a cytoplasmic pattern recognition receptor (prr) and is a member of nod like receptor (nlr) family. it senses a wide range of bacteria and viruses or their products and is involved in innate immune responses. in this report, nod-2 gene was cloned and characterized from rohu (labeo rohita) which is highly commercially important fish species in the indian subcontinent. the full length rohu nod-2 (rnod-2) cdna comprised of 3176bp with a singl ...201121767564
inhibition of atpase activity in the freshwater fish labeo rohita (hamilton) exposed to sodium cyanide.present study concerns the effect of sodium cyanide on the indian major carp, labeo rohita. fishes were exposed to lethal (0.32?mg/l) and sublethal (0.064?mg/l) concentrations of sodium cyanide. the effect of intoxication was studied on na(+)k(+)atpase, mg(+2)atpase and ca(2+)atpase in various physiological tissues (gill, liver, and muscle) at the end of 1, 2, 3 and 4 days of lethal and 5, 10 and 15 days of sublethal exposure periods. sodium cyanide induced significant inhibitory effects on the ...201121777177
oxidative damage to lipids and proteins induced by aflatoxin b(1) in fish (labeo rohita)-protective role of amrita bindu.in the present study, fish (labeo rohita) were treated with a single intraperitoneal administration of aflatoxin b(1) (afb(1)) (100++g/100gbw). the resultant oxidative damage to lipids (measured as conjugated diene and lipid peroxidation (lpo)) and proteins (protein carbonyl) in liver, kidney and brain at the end of 3rd and 6th day was assessed. our results showed that afb(1) induced a significant increase in conjugated diene formation and lpo not only in liver but also in kidney and brain. a pa ...200421782716
cloning of cdna and prediction of peptide structure of plzf expressed in the spermatogonial cells of labeo rohita.the promyelocytic leukemia zinc finger (plzf) gene containing an evolutionary conserved btb (bric-a-brac/tramtrack/broad complex) domain plays a key role in self-renewal of mammalian spermatogonial stem cells (sscs) via recruiting transcriptional co-repressors. little is known about the function of plzf in vertebrate, especially in fish species. to gain better understanding of its role in fishes, we have cloned plzf from the testis of labeo rohita (rohu), a commercially important freshwater carp ...201021798210
modulation in the protein metabolism by subacute sodium cyanide intoxication in the freshwater fish, labeo rohita (hamilton).the effects of exposure to one-third and one-fifth sublethal concentrations (0.106 and 0.064ôçëmg/l) of sodium cyanide on protein metabolism on freshwater carp, labeo rohita, was studied. three functionally different tissues, namely, the liver, muscle, and gills, were studied after 5, 10, and 15 days. exposures produced marked changes in protein metabolic profile in all tissues studied. these changes were more pronounced in the one-third sublethal concentration, suggesting a cumulative action of ...201121830916
Effect of alternate day feeding strategy of sub-optimal protein level on haemato-biochemical responses in Labeo rohita (Hamilton) juveniles.A feeding trial was conducted for 60 days to delineate the effect of alternate day feeding strategy of sub-optimal protein level on haematological parameters, serum parameters and phagocyte respiratory burst activity (NBT) in Labeo rohita juveniles. One hundred and thirty-five fingerlings (1.87 ± 0.01-2.26 ± 0.05 g) were distributed in triplicate groups of each treatment, and fish were fed at 5% body weight daily. Three experimental isocaloric (401.32-410.28 kcal/100 g) diets of 30%, 25%, and 20 ...201121883499
antigen adsorbed calcium phosphate nanoparticles stimulate both innate and adaptive immune response in fish, labeo rohita h.calcium phosphate nanoparticles as an antigen/protein delivery was explored in a fish model labeo rohita h. s-layer protein (of aeromonas hydrophila) adsorbed on nano sized calcium phosphate particles elicited both innate and adaptive immune parameters which persisted up to 63 days of post immunization through parenteral immunization and gave cross protections.201121889128
dose dependence specific and non-specific immune responses of indian major carp (l. rohita ham) to intraperitoneal injection of formalin killed aeromonas hydrophila whole cell vaccine.specific and non-specific immune response to different doses of formalin killed whole cell vaccine of aeromonas hydrophila to indian major carp (labeo rohita) was evaluated in laboratory condition. three different doses (10(5) cfu/ml, 10(7) cfu/ml, 10(10) cfu/ml) were administered (0.2 ml/fish) intraperitoneally for 1 month. among the three doses, 10(10) cfu/ml elicited the highest antibody and protective response followed by the doses 10(7) cfu/ml and 10(5) cfu/ml. upon challenge with the virul ...201121901335
study of the effects of the casein derived bitter tastant on the melanophores in milieu with the melatonin receptors.the present study was undertaken to ascertain whether the casein derived bitter tastant cyclo (leu-trp) [clt] has an affinity or not for the particular receptors of the pineal hormone, melatonin, on the melanophores of a major carp labeo rohita (ham.). the bitter tastant clt, in the dose range of 3.34×10(-16) m to 3.34×10(-4) m, has induced an aggregatory effect but not in a dose dependent manner. binding of clt with the receptors may vary at different concentrations. denervation of the melanoph ...201121929292
Differential resistance to edwardsiellosis in rohu (Labeo rohita) families selected previously for higher growth and/or aeromoniasis-resistance.Selection in fish for disease resistance is one of the most useful approaches to solve disease problems. Genetic variation in resistance to edwardsiellosis in fullsib families of rohu, Labeo rohita was investigated in the present study. A large variation in the susceptibility pattern (0 to 94.74 percent survival) against Edwardsiella tarda challenge was observed among 57 families. Additive genetic variation showed a heritability of 0.38?±?0.08 across the year-class survival. The apparent resista ...201122012677
Dietary choline, betaine and lecithin mitigates endosulfan-induced stress in Labeo rohita fingerlings.A five-week experiment was conducted to delineate stress-mitigating effects of three different methyl donors in Labeo rohita fingerlings subjected to endosulfan toxicity. Four iso-nitrogenous and iso-caloric feed were prepared with and without supplementation of methyl donors. The feed were basal or control diet (i.e., without methyl donor supplementation), feed supplemented with choline, feed supplemented with betaine and feed supplemented with lecithin. Two hundred and twenty-five fishes were ...201122160664
Molecular characterization of toll-like receptor 2 (TLR2), analysis of its inductive expression and associated down-stream signaling molecules following ligands exposure and bacterial infection in the Indian major carp, rohu (Labeo rohita).Toll-like receptors (TLRs) are one of the key components of innate immunity. Among various TLR types, TLR2 is involved in recognizing specific microbial structures such as peptidoglycan (PGN), lipoteichoic acid (LTA), zymosan etc., and after binding them it triggers myeloid differentiation primary response gene 88 (MyD88)-dependent signaling pathway to induce various cytokines. In this report, TLR2 gene was cloned and characterized in rohu (Labeo rohita), which is highly commercially important f ...201122173272
expressed sequences and polymorphisms in rohu carp (labeo rohita, hamilton) revealed by mrna-seq.expressed genes and polymorphisms were identified in lines of rohu labeo rohita selected for resistance or susceptibility to aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis. all animals were grown in a common environment and rna from ten individuals from each line pooled for illumina mrna-seq. de novo transcriptome assembly produced 137,629 contigs with 40× average coverage.forty-four percent of the assembled sequences were annotated with gene names and ontology terms. ...201222298294
response of the chromatophores in relation to the healing of skin wounds in an indian major carp, labeo rohita (hamilton).chromatophores show significant changes during healing of skin wounds in labeo rohita (common name--rohu). wound area can be divided into regions i, ii and iii. after infliction of wound, skin colour becomes significantly dark by 2h that is gradually restored by 2d. in regions ii and iii at 5 min, epidermal melanophores appear with beaded dendrites. in these regions at 2h and in region i at 6h, epidermal melanophores appear small, rounded or irregular shaped having dendritic processes with aggre ...201222321648
comparative analysis of innate immune parameters of the skin mucous secretions from certain freshwater teleosts, inhabiting different ecological niches.the innate immune system of fish is considered first line of defense against a broad spectrum of pathogens. being a component of innate immunity and lying at the interface between fish and the aqueous environment, skin mucus plays a frontier role in protecting fish from infections. in the present study, skin mucus of cirrhinus mrigala, labeo rohita, catla catla, rita rita and channa punctata, inhabiting different ecological niches, was analyzed to characterize potential innate immune factors suc ...201222350522
effects of dietary supplementation of potential probiotic pseudomonas aeruginosa vsg-2 on the innate immunity and disease resistance of tropical freshwater fish, labeo rohita.the effects of dietary pseudomonas aeruginosa vsg-2 supplementation on innate immunity and protection against aeromonas hydrophila infection were evaluated in labeo rohita. fish were fed for 60 days with control diet or 3 experimental diets containing p. aeruginosa vsg-2 at 10(5), 10(7), and 10(9) cfu g(-l), respectively. various innate immune parameters were examined at 30 and 60 days post-feeding. fish were challenged with a. hydrophila 60 days post-feeding and mortalities were recorded over 1 ...201222465361
molecular cloning and characterization of nucleotide binding and oligomerization domain-1 (nod1) receptor in the indian major carp, rohu (labeo rohita), and analysis of its inductive expression and down-stream signalling molecules following ligands exposure and gram-negative bacterial infections.nucleotide binding and oligomerization domain-1 (nod1) is a cytoplasmic pattern recognition receptor (prr), and is a member of the nod-like receptor (nlr) family. it senses a wide range of bacteria and viruses or their products, and plays a key role in inducing innate immunity. in this report, nod1 gene was cloned and characterized in rohu (labeo rohita), a fish species of highest commercial importance in the indian subcontinent. the full-length rohu nod1 (rnod1) cdna comprised of 3168 bp with a ...201222530240
recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda.the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ...201222564558
impact of cypermethrin on fingerlings of common edible carp (labeo rohita).laboratory evaluations were made to assess the toxicological and biochemical effect of cypermethrin on fingerlings of common edible freshwater culture carp (labeo rohita). there was a significant negative (p < 0.05) correlation observed between effective doses of cypermethrin and exposure periods; that is, lc₅₀ values decreased from 0.323 μg/l (6 h) to  > 0.278 μg/l (12 h),  > 0.240 μg/l (18 h) and >0.205 μg/l (24 h). exposure to sublethal doses of cypermethrin for 24 h and 96 h exposure period ...201222666103
efficacy of two avermectins, doramectin and ivermectin against argulus siamensis infestation in indian major carp, labeo rohita.the efficacy of two avermectins, doramectin and ivermectin via oral and parenteral routes and their prophylactic effects against argulus siamensis infestation in indian major carp, labeo rohita was studied. doramectin and ivermectin were fed once to l. rohita sub-adults naturally infested with a. siamensis parasites at graded levels of 1000, 750 and 500 μg/kg body weight and 500, 300 and 200 μg/kg body weight, respectively. the mean time of clearance of parasites was 24 ± 0.149 h for 1000 and 24 ...201222673107
development of an es-like cell culture system (resc) from rohu, labeo rohita (ham.).an embryonic stem (es)-like cell culture system resc from a commercially important freshwater carp, labeo rohita, was developed using blastula stage embryos. the cells were cultured in leibovitz-15 (l-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basic fibroblast growth factor at 28 °c under feeder-free conditions. the es-like cells were characterized by their unique morphology, alkaline phosphatase activity, embryoid body forma ...201222707190
histopathological effects and bioaccumulation of mercury in the kidney of an indian major carp, labeo rohita (hamilton).the effect of mercuric chloride on the histo-morphology and bioaccumulation in the kidney of an indian major carp, labeo rohita (hamilton) were examined after exposing the fish (15-20 cm) to three sublethal concentrations (0.033, 0.066 and 0.132 mg/l) of hgcl(2) for 30 days. mercury deposition in kidney tissues had increased significantly with dose and exposure duration dependant manner. several histological changes were noted in the kidney of all treated groups in compare to control group.201222810019
nutrients budget and effluents characteristics in polyculture of scampi (macrobrachium rosenbergii) and indian major carps ponds using organic inputs.experiments were conducted for the study of nutrient budget in farmers' ponds (0.4-0.6 ha) in orissa, india, at stocking densities of 0.30-0.38/m(2) for scampi (macrobrachium rosenbergii) and 0.60-0.70/m(2) for indian major carps (catla catla, labeo rohita and cirrhinus mrigala). the average initial body weights of scampi and the major carps were 0.02 and 8-10 g, respectively. the culture period was 272-292 days. feed was the main input. the fcr (feed conversion ratio) varied from 1.78 to 1.83. ...201222864442
the first evidence of cholinesterases in skin mucus of carps and its applicability as biomarker of organophosphate exposure.the presence of cholinesterase (che) activity in skin mucus of three carps, cirrhinus mrigala, labeo rohita, and catla catla and its applicability as biomarker of the organophosphorus insecticide exposure were investigated. biochemical characterization, using specific substrates and inhibitors, indicated that measured esterase activity in skin mucus was mainly owing to ches. significant difference in the proportion of acetylcholinesterase and butyrylcholinesterase activities was observed in skin ...201422887814
histochemical analysis of glycoproteins in the secretory cells in the epidermis of the head skin of indian major carp, labeo rohita.a series of histochemical procedures were employed to localise and characterise glycoprotein (gp) classes produced by the epithelial cells, the type a and the type b mucous goblet cells (mgcs) and the club cells in the epidermis of labeo rohita. the epithelial cells secreted gps with oxidizable vicinal diols and gps with sialic acid residues without o-acyl substitution in low concentrations. the type a mgcs and the type b mgcs, in contrast, produced these gps in high concentrations. further, the ...201222939776
escherichia coli contamination of fish raised in integrated pig-fish aquaculture systems in vietnam.integrated livestock-fish aquaculture utilizes animal excreta and urine as pond fertilizers to enhance growth of plankton and other microorganisms eaten by the fish. in vietnam, pigs are commonly integrated with fish and horticulture in household-based vac systems (vuon = garden; ao = pond; chuong = pigsty), but the level of fecal contamination in the fish produced is unknown. this study was carried out to assess the level escherichia coli contamination of fish meat and gut contents of grass car ...201222980016
monoclonal antibody to serum immunoglobulins of clarias batrachus and its application in immunoassays.serum immunoglobulins of clarias batrachus (cb-ig) were purified by affinity chromatography using bovine serum albumin as capture ligand. under reducing conditions in sds-page, cb-ig was composed of a heavy (h) chain (68.7 kda) and two light (l) chains (27.4 and 26.3 kda). purified cb-ig was used to produce a monoclonal antibody (mab) designated e4 mab that belonged to igg1 subclass. in western blotting, this mab showed binding to h chain of purified cb-ig and putative h chains in reduced sera o ...201223000018
toxicity of crude extracellular products of aeromonas hydrophila on rohu, labeo rohita (ham.).in the present study the haemolytic and proteolytic activity of extracellular products (ecp) secreted from aeromonas hydrophila (cahh14 strain) were studied with respect to temperature and different time of incubation as well as its lethal toxicity on rohu, labeo rohita. the strain was isolated from catla catla (showing abdominal dropsy symptom) collected from the pond of central institute of freshwater aquaculture (cifa), bhubaneswar, india and was characterized on the basis of biochemical test ...201123024416
two new species of myxobolus (myxozoa: myxosporea: bivalvulida) infecting an indian major carp and a cat fish in wetlands of punjab, india.a survey of parasites of freshwater fishes in kanjali and harike wetlands of punjab (india) revealed the presence of two new myxosporean species belonging to the genus myxobolus bütschli (winter (ed): protozoa, 1882). spores of the first species, m. saugati sp. nov. parasitizing scales of labeo rohita (cypriniformis: cyprinidae) vern. rohu are characterized in having 'spores measuring 8.3 × 6.6 μm in size, oval to spherical in valvular view having rounded anterior and posterior ends; polar capsu ...201123024499
effect of dietary supplementation with achyranthes aspera seed on larval rohu labeo rohita challenged with aeromonas hydrophila.larval rohu labeo rohita were fed four different diets: three of the diets contained achyranthes aspera (prickly chaff-flower) seeds at 0.10% (d1), 0.25% (d2), or 0.50% (d3); the fourth diet was a control diet (d4; no a. aspera supplementation). after 70 d, the rohu were injected intraperitoneally with live aeromonas hydrophila. mortality of fish was recorded for 7 d. in the d4 group, the first mortality was observed within 12 h of exposure, whereas in the d1-d3 treatment groups, mortality was f ...201223025591
effects of water borne iron on spawn of indian major carps (catla catla (ham.), labeo rohita (ham.) and cirrhinus mrigala (ham.)).effects of water-borne iron on indian major carps spawn were evaluated in the present study. ferrous sulphate was used to prepare different test iron concentrations. mrigal had the lowest 96 h lc(50) value of 0.30 ± 0.06 mg l(-1) while rohu had the highest value of 0.73 ± 0.06 mg l(-1) of iron. accumulation of iron in mrigal spawn was highest whereas it was lowest in catla. abnormal behaviour and reduced growth were observed in chronic toxicity. application factors were calculated to establish a ...201223052582
molecular cloning and characterization of toll-like receptor 3, and inductive expression analysis of type i ifn, mx and pro-inflammatory cytokines in the indian carp, rohu (labeo rohita).toll-like receptors (tlrs) are one of the key components of innate or non-specific immunity. among various types of tlrs, tlr3 recognizes dsrna, the genetic material or replicative intermediate of many rna viruses and triggers tir-domain-containing adapter-inducing interferon-β dependent signalling pathway to induce type i interferon (ifn) and pro-inflammatory cytokines. in this study, we cloned and characterized full-length tlr3 cdna in rohu (labeo rohita), that comprised of 2,619 bp nucleotide ...201323065215
peptide mapping of polymorphic myosin heavy chain isoforms in different muscle types of some freshwater teleosts.a modified sds-page system has been employed to resolve polymorphic myosin heavy chain (myhc) isoforms in different muscle types of three freshwater teleosts displaying different modes of respiration, adaptive features and life styles. investigated species include accessory air-breather channa punctata along with exclusive aquatic breather major carps labeo rohita and catla catla. all the selected species show specificity and expressivity of at least three myhc isoforms, one each in red, head an ...201323086608
characterization of vibrio species isolated from freshwater fishes by ribotyping.three vibrio species from the resident microflora of gastrointestinal tract of freshwater carps and prawns were isolated and confirmed biochemically as v. fluvialis from cyprinus carpio/labeo rohita; v. parahaemolyticus from macrobrachium rosenbergii and v. harveyi from macrobrachium malcomsoni. the genetic relationship among these vibrio species was carried out by polymerase chain reaction (pcr) amplification of 16s rrna gene followed by restriction digestion with hae iii, bam hi and pst i. den ...201023100815
enhanced anti-proteases inlabeo rohita fed with diet containing herbal ingredients.aqueous root extract ofachyranthes aspera was incorporated in the experimental diet oflabeo rohita (rohu). control diet was prepared without root extract. feeding of fishes with experimental diet has significantly (p<0.05) enhanced the serum anti-proteases level than fishes fed with control diet.200423105471
toxicological effects of the antibiotic oxytetracycline to an indian major carp labeo rohita.oxytetracycline (otc), an antibacterial agent, is extensively used in aquaculture practices all over the world. despite its use, the toxicity of otc to freshwater fish has been scarcely investigated. in this study, labeo rohita were exposed to different concentrations (20, 40, 60, 80, 100, and 120 mg l(-1)) of otc. based on the survival-to-mortality ratio, an 80 mg l(-1) concentration was selected for sublethal toxicity analysis. fish were exposed to the above-mentioned concentration for a perio ...201323192588
establishment and characterization of permanent cell line from gill tissue of labeo rohita (hamilton) and its application in gene expression and toxicology.rohu gill cell line (lrg) was established from gill tissue of indian major carp (labeo rohita), a freshwater fish cultivated in india. the cell line was maintained in leibovitz's l-15 supplemented with 10 % foetal bovine serum (fbs). this cell line has been sub-cultured more than 85 passages over a period of 2 years. the lrg cell line consists of both epithelial and fibroblastic-like cells. the cells were able to grow at a wide range of temperatures from 22 to 32 °c, the optimum temperature bein ...201323224722
studies on trichodinid and chilodonellid ciliophorans (protozoa: ciliophora) in the indian freshwater and estuarine fishes with description of trichodinella sunderbanensis sp. nov. and trichodina nandusi sp. nov.a comprehensive icthyoparasotological survey among estuarine and freshwater fishes of west bengal, india revealed trichodinella sunderbanensis sp. nov. and trichodina acuta lom, 1961 from an estuarine fish mystus gulio (hamilton, 1822) from the vidyadhari river; trichodina nandusi sp. nov. from a freshwater fish, nandus nandus (hamilton-buchanan); chilodonella hexasticha (kiernik, 1909) kahl, 1931 from freshwater fishes labeo rohita (hamilton, 1822); and cyprinus carpio linnaeus, 1758 from the r ...201223269510
potential probiotic lactobacillus plantarum vsg3 improves the growth, immunity, and disease resistance of tropical freshwater fish, labeo rohita.the study evaluated the effects of dietary doses of lactobacillus plantarum vsg3 on the growth performance, immunity, and disease resistance of labeo rohita juveniles against aeromonas hydrophila infection. fish (mean body weight 60 g) were fed with diet containing 0 (control), 10(6), 10(8), 10(10) cfu g(-1)l. plantarum vsg3 for 60 days. various growth and immune parameters were examined at 30 and 60 days of post-feeding. fish were challenged with a. hydrophila 60 days post-feeding and mortaliti ...201223274158
computational analysis of transcriptome of indian major carp, labeo rohita (hamilton-buchanan, 1822) for functional annotation.a total of 1671 ests of labeo rohita were retrieved from dbest database and analysed for functional annotation using various computational approaches. the result indicated 1387 non-redundant (184 contigs and 1203 singletons) putative transcripts with an average length of 542 bp. these 1387 transcript sequences were matched with refseq_rna, unigene and swiss-prot on high threshold cut-off for functional annotation along with help of gene ontology and ssrs markers. we developed extensive perl prog ...201223275698
relationship between snail population density and infection status of snails and fish with zoonotic trematodes in vietnamese carp nurseries.fish-borne zoonotic trematodes (fzt) are a food safety and health concern in vietnam. humans and other final hosts acquire these parasites from eating raw or under-cooked fish with fzt metacercariae. fish raised in ponds are exposed to cercariae shed by snail hosts that are common in fish farm ponds. previous risk assessment on fzt transmission in the red river delta of vietnam identified carp nursery ponds as major sites of transmission. in this study, we analyzed the association between snail ...201223285303
analysis of lactate and malate dehydrogenase enzyme profiles of selected major carps of wetland of calcutta.the east calcutta wetland (ecw), a ramsar site in india, acts as the only sink for both city sewages as well as effluents from the surrounding small-scale industries and is alarmingly polluted with heavy metals. the three best edible major carp species rohu (labeo rohita,), catla (catla catla,) and mrigala (cirrhinus mrigala) were undertaken to monitor lactate dehydrogenase (ldh) and malate dehydrogenase (mdh) by cellulose acetate electrophoresis (cae) to assess the effects of pollutants, if any ...201223360005
contamination of the river ganga and its toxic implication in the blood parameters of the major carp labeo rohita (ham).a field study was conducted to examine different physicochemical properties of water and various haematological and biochemical parametres of the fish labeo rohita collected from the ganga river (national river of india) at varanasi district, india. the water was found to be greatly contaminated with a number of dissolved metals (fe, cr, zn, cu, mn, ni and pb) whose concentrations were above the safe limits suggested by bureau of indian standard (bis 1991) for drinking water (fe, 1,353.33 %; cr, ...201323456946
characterization of the ceruloplasmin gene and its potential role as an indirect marker for selection to aeromonas hydrophila resistance in rohu, labeo rohita.ceruloplasmin is an acute phase protein found to be activated by the host immune system during stress conditions. the ceruloplasmin gene has been reported in several teleosts and here we characterize the gene and test its association with resistance to aeromonas hydrophila in rohu, labeo rohita. a ceruloplasmin mrna sequence of 3355 base pairs (bp) was derived (genbank id: jx010736). the coding sequence (cds) comprised of 3276 bp that coded for 1092 amino acids. alignment results showed the grea ...201323481214
record of the species of tripartiella (lom, 1959) from fishes of manipur.survey on trichodinid ciliophorans from the fresh water fishes of manipur revealed three known species of the genus tripartiella from the gills of major carps labeo rohita (hamilton); cirrhinus mrigala (hamilton); catla catla and ciprinus carpio. these are redescribed in this communication.201223542123
parasitic incidence in a cyprinid fish labeo rohita (ham.) at river song in doon valley (uttarakhand).on examining 144 specimens of labeo rohita (ham.) for parasites occurrence; as many as 34 fish were found positive to harbour ciliophorans (trichodina sp. and ichthyophthirius multifilis fouquet, 1876), monogeneans (haplocleidus vachi tripathi, 1959 and dactylogyrus glossogobii jain, 1960), trematode (allocreadium mahaseri pandey, 1939), nematode (camallanus (zeylanema) anabantis pearse, 1933) and acanthocephalan (sachalinorhynchus sp). highest prevalence was shown by platyhelminthes (18.75%) fo ...201123542706
lipid classes and fatty acid profile of selected indian fresh water fishes.lipid extracts from meat, head and viscera of indian fresh water fishes, viz., catla, rohu, mrigal, common carp and tilapia were analyzed for lipid class distribution and fatty acid profile. the yield of meat ranged from 66.0-79.5% and total lipid content in meat was 0.8-3.8%. the total lipid content was higher (>4.0%) in head and viscera. neutral lipids constituted 71.5-93.3% of the total lipid extract. higher glycolipid content of 25.2% was observed in lipid extract from meat of common carp an ...201023572659
identification of reproduction-related genes and ssr-markers through expressed sequence tags analysis of a monsoon breeding carp rohu, labeo rohita (hamilton).labeo rohita (ham.) also called rohu is the most important freshwater aquaculture species on the indian sub continent. monsoon dependent breeding restricts its seed production beyond season indicating a strong genetic control about which very limited information is available. additionally, few genomic resources are publicly available for this species. here we sought to identify reproduction-relevant genes from normalized cdna libraries of the brain-pituitary-gonad-liver (bpgl-axis) tissues of ad ...201323583682
characterization and physical mapping of 18s and 5s ribosomal genes in indian major carps (pisces, cyprinidae).characterization of the major (18s) and minor (5s) ribosomal rna genes were carried out in three commercially important indian major carp (imc) species, viz. catla catla, labeo rohita and cirrhinus mrigala along with their physical localizations using dual colour fluorescence in situ hybridization. the diploid chromosome number in the above carps was confirmed to be 50 with inter-species karyo-morphological variations. the 18s rdna signals were observed on 3 pair of chromosomes in c. catla and l ...201323587674
use of black carp (mylopharyngodon piceus) in biological control of intermediate host snails of fish-borne zoonotic trematodes in nursery ponds in the red river delta, vietnam.the risks of fish-borne zoonotic trematodes (fzt) to human health constitute an important problem in vietnam. the infection of humans with these trematodes, such as small liver trematodes (clonorchis sinensis and opisthorchis viverrini), intestinal trematodes (heterophyidae) and others is often thought to be linked to fish culture in areas where the habit of eating raw fish is common. juvenile fish produced in nurseries are often heavily infected with fzt and since fishes are sold to aquaculture ...201323680382
review of the bioenvironmental methods for malaria control with special reference to the use of larvivorous fishes and composite fish culture in central gujarat, india.mosquito control with the use of insecticides is faced with the challenges of insecticide resistance in disease vectors, community refusal, their high cost, operational difficulties, and environmental concern. in view of this, integrated vector control strategies with the use of larvivorous fishes such as guppy (poecilia reticulata) and gambusia (g. affinis) as biological control agents were used in controlling mosquito breeding in different types of breeding places such as intradomestic contain ...201323703433
prostanoids-induced dispersion in the melanophores of a carp labeo rohita (ham.).effects of three prostaglandins (i.e., prostanoids) and one of its precursors, arachidonic acid, were examined on the melanophores of the fish labeo rohita (ham.). pge₁, pge₂, pgf(2α) and arachidonic acid elicit a concentration-related dispersion in the fish melanophores. in vitro analysis of melanophores was performed through incubation of the isolated fish scales in different agonists and antagonists solutions. dispersal effect of prostanoids may be mediated directly through the typical recept ...201423801365
thermosensitive ion channel trpv1 is endogenously expressed in the sperm of a fresh water teleost fish (labeo rohita) and regulates sperm motility.sperm cells exhibit extremely high sensitivity in response to slight changes in temperature, osmotic pressure and/or presence of various chemical stimuli. in most cases throughout the evolution, these physico-chemical stimuli trigger ca ( 2+) -signaling and subsequently alter structure, cellular function, motility and survival of the sperm cells. few reports have recently demonstrated the presence of transient receptor potential (trp) channels in the sperm cells from higher eukaryotes, mainly fr ...201323912940
development and characterization of a continuous macrophage cell line, lrtm, derived from thymus of labeo rohita (hamilton 1822).a long-term thymic macrophage cell line from the thymus explants of labeo rohita designated as lrtm (l. rohita thymic macrophages) was established, which has been maintained in culture for more than 1 yr. this cell line designated lrtm cells have been subcultured for 70 passages. the cells shape was initially long and elongated; with subsequent passages, the cells became short and epithelial like. the cells exhibited optimum growth in l-15 containing 10% fetal bovine serum and also in dulbecco's ...201423955429
effect of dietary supplemented andrographolide on growth, non-specific immune parameters and resistance against aeromonas hydrophila in labeo rohita (hamilton).the present study evaluated the effect of dietary andrographolide (ec 50%) on growth, non-specific immune parameters and disease resistance against aeromonas hydrophila infection in indian major carp, labeo rohita fingerlings. fishes were fed with formulated diet containing andrographolide as t0 (0.00%), t1 (0.05%), t2 (0.10%), t3 (0.20%), t4 (0.40%) and t5 (0.80%) for 42 days. fishes were challenged with a. hydrophila 42 days post feeding and relative percentage survival (rps) was recorded over ...201323973382
a comparative study of coi and 16 s rrna genes for dna barcoding of cultivable carps in india.the 5' region of the mitochondrial dna gene cytochrome c oxidase subunit i (coi) is the standard marker for dna barcoding. however, 16 s rrna has also been advocated for dna barcoding in many animal species. herein, we directly compare the usefulness of coi and 16 s rrna in discriminating six cultivable carp species: labeo rohita, catla catla, cirrhinus mrigala, labeo fimbriatus, labeo bata and cirrhinus reba from india. analysis of partial sequences of these two gene fragments from 171 individu ...201524047160
fishmicrosat: a microsatellite database of commercially important fishes and shellfishes of the indian subcontinent.microsatellite dna is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. the biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. the microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. the system also helps to design primer sequences for flank ...201324047532
hematological, biochemical and enzymological responses in an indian major carp labeo rohita induced by sublethal concentration of waterborne selenite exposure.selenium (se) pollution in aquatic ecosystem is an environmental issue throughout the world. elevated concentrations of inorganic se from agricultural and industrial processes may cause adverse biological effects in aquatic organisms such as fish. in the present study, labeo rohita an indian major carp were exposed to sublethal concentration of se (sodium selenite) for 35days and certain hematological, biochemical and enzymological parameters were estimated. the median lethal concentration of wa ...201424183823
present status and approaches for the sustainable development of community based fish culture in seasonal floodplains of bangladesh.coordination among the different stakeholders at policy planning, implementation and target beneficiary level, particularly among the agencies responsible for development and management of water resources, agriculture and fisheries, is essential for overall sustainable development. stocking of larger fingerlings at suitable stocking densities of endemic (rohu, catla, mrigal) and exotic (silver carp, bighead carp, common carp/mirror carp) species should be stocked at varying proportion. floodplai ...201224191617
impact of exotic carps in the polyculture with indigenous carps: competition for food.the fingerlings of indigenous carps such as catla (catla catla), rohu (labeo rohita) and mrigal (cirrhinus mrigala) with exotic carps such as silver carp (hypophthalmichthys molitrix), bighead carp (aristichthys nobilis) and mirror carp (cyprinus carpio) were cultured together in a fish pond at bangladesh agricultural university, mymensingh, in order to determine the food electivity, dietary overlap and food competition among indigenous major carps and exotic carps. phytoplankton (chlorophyceae, ...201224191618
identification and characterization of maturation-promoting factor from catfish,clarias batrachus.maturation-promoting factor (mpf) extracted from maturing oocytes of catfishes (clarias batrachus andheteropneustes fossilis) and carp (labeo rohita) induces 100% germinal vesicle breakdown (gvbd) when microinjected intoclarias immature unstimulated oocytes. the presence of a similar mpf activity has also been demonstrated in the active fractions collected after superose 12. sds-page analyses of cytosolic extracts (ce) prepared from immature and mature oocytes revealed the presence of 34- and 46 ...199624194144
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