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sublethal temperature stress in juvenile labeo rohita (ham-buch.) and rita rita (ham.): some physiological changes.juveniles of fish l. rohita and r. rita subjected to a rapid (5 min) sublethal temperature increase from 28 to 35 degrees c showed significant increase in cortisol and decrease in interrenal ascorbic acid. hypercholesterolemia, hyperglycemia and hyperlactemia were also evident accompanied by increased blood haemoglobin and haematocrit and stable protein levels. compensatory responses were initiated within 72 hr in both the fishes. r. rita recovered more quickly indicating it to be more resistant ...200212622207
stage-dependent hatching responses of rohu (labeo rohita) embryos to different concentrations of cryoprotectants and temperatures.hatching performances of three embryonic stages of postfertilization rohu (labeo rohita) (9-, 12-, and 15-h) were examined after treatment with various concentrations (0.5-4.5m) of two cryoprotectants (methanol and propylene glycol) supplemented with 0.1m trehalose. different lengths of storage (1-48 h) and temperature (-4 degrees c to ambient) were studied. of the three stages of embryonic development, the 12-h stage proved to be the most suitable stage for low temperature storage, showing the ...200312623023
studies of some biochemical changes in the tissues of catla catla (hamilton), labeo rohita (hamilton) and cirrhinus mrigala (hamilton) exposed to nh3-n, no2-n and no3-n.biochemical changes, total proteins, glycogen, aspartate and alanine (aat and alat) amino transferases were studied with exposure of sublethal concentrations of nh3-n, no2-n and no3-n to the freshwater fish catla catla (hamilton), labeo rohita (hamilton) and cirrhinus mrigala (hamilton). depletion in the food reserves and enzyme activity was observed in all the three fish species exposed to these toxicants. hence, the concentrations of nh3, no2 and no3 in water need to be monitored in water qual ...200212674377
incorporation of brewery waste in supplementary feed and its impact on growth in some carps.brewery waste (brewer's grains) was used at four different levels (10%, 20%, 30% and 40% w/w) replacing rice bran in fish diet under a semi-intensive culture system and its impact on the growth of catla, catla catla; rohu, labeo rohita and mrigal, cirrhina mrigala, was studied. growth in terms of body weight gain was maximum in c. catla and l. rohita fed on a diet containing 30% brewery waste in the feed, whereas c. mrigala, fed on a diet containing brewery waste at the above mentioned levels sh ...200414585627
the immunosuppressive effect of alpha-permethrin on indian major carp, rohu (labeo rohita ham.).the immunosuppressive effects of bath exposure to a sub lethal concentration of the synthetic pyrethroid alpha-permethrin (3.05 x 10(-4) mg l(-1)) in the indian major carp, labeo rohita was studied after 45 days' exposure. in some groups, the effects of alpha-permethrin on non-specific defences and serum enzymes of carp were investigated after challenge with aeromonas hydrophila. several nonspecific immune responses and serum enzymes were reduced after exposure of alpha-permethrin. bactericidal ...200414675832
purification and properties of cytosolic alanine aminotransferase from the liver of two freshwater fish, clarias batrachus and labeo rohita.cytosolic alanine aminotransferase (c-aat) was purified up to 203- and 120-fold, from the liver of two freshwater teleosts clarias batrachus (air-breathing, carnivorous) and labeo rohita (water-breathing, herbivorous), respectively. the enzyme from both fish showed similar elution profiles on a deae-sephacel ion exchange column. sds-page of purified enzymes revealed two subunits of 54 and 56 kda, in both fish. the apparent km values for l-alanine were 18.5+/-0.48 and 23.55+/-0.60 mm, whereas for ...200414990216
toxic and sub-lethal effects of oleandrin on biochemical parameters of fresh water air breathing murrel, channa punctatus (bloch.).active compound oleandrin extracted from nerium indicum (lal kaner) leaf has potent piscicidal activity. the piscicidal activity of oleandrin on freshwater fish c. punctatus was both time and dose dependent. exposure to sub-lethal doses of oleandrin for 24hr and 96hr to fish caused significant alteration in the level of total protein, total free amino acid, nucleic acid, glycogen, pyruvate, lactate and enzyme protease, phosphatases, alanine aminotransferase, aspartate aminotransferase and acetyl ...200415088692
effects of cypermethrin and carbofuran on certain hematological parameters and prediction of their recovery in a freshwater teleost, labeo rohita (hamilton).sublethal effects of cypermethrin and carbofuran on hematological parameters and their complete recovery were studied in labeo rohita as a function of exposure time. exposure of labeo rohita to sublethal levels of cypermethrin and carbofuran resulted in significantly (p<0.05) lower values for erythrocyte count (rbc), hemoglobin content (hb), and hematocrit compared with the control group. in contrast, there was a significant increase (p<0.05) in leukocyte count (tlc) in the pesticide-treated gro ...200415157576
growth enhancement and food conversion efficiency of transgenic fish labeo rohita.three family lines of fast growing transgenic rohu labeo rohita (rohu) were generated by electroporated-sperm-mediated transfer of the vectors harboring cmv promoter or grass carp beta-actin promoter fused to endogenous rohu gh (rgh) cdna. the gene transfer efficiency was 25%. the transgenic rohu (family line 1) with cmv promoter showed a growth enhancement of four times normal size, whereas those (family lines 2 and 3) generated with beta-actin promoter grew 4.5 and 5.8 times faster than their ...200415181642
nitrite toxicity in indian major carps: sublethal effect on selected enzymes in fingerlings of catla catla, labeo rohita and cirrhinus mrigala.the effects of 96-h sublethal exposure of nitrite (1, 2, 4, 8 and 10.4 mg l(-1)) on selected enzymatic activities in serum and tissues of fingerlings of catla (catla catla), rohu (labeo rohita) and mrigal (cirrhinus mrigala) were studied for the first time in these species. all three species responded almost identically to nitrite exposure. with increasing nitrite concentration, reduction in activities was observed in acetylcholinesterase (ache) in brain and liver; alkaline phosphatase (alp) in ...200415313441
interference of magnesium on zinc adsorption by pond sediment and on zinc accumulation in a freshwater teleost, labeo rohita (hamilton).two experiments were conducted to evaluate the interference of magnesium on zinc adsorption in freshwater fish pond sediment and zinc accumulation in the freshwater teleost labeo rohita (hamilton). the study demonstrated that increasing the concentration of magnesium as magnesium sulfate from 100 to 400 mg kg(-1) decreased the adsorption of zinc in sediment. the maximum adsorption was found at a concentration of 100 mg kg(-1) of mg(2+) and the minimum with 400 mg kg(-1) of mg(2+). this study als ...200415327880
conversion efficiency and nutrient digestibility of certain seaweed diets by laboratory reared labeo rohita (hamilton).impact of three different types of seaweed diets on growth, feed utilization and nutrient digestibility of l. rohita was studied for 120 days. the seaweed diet fed fishes, especially ulva based diet showed comparatively higher growth and weight increment. good food conversion ratio, food assimilation efficiency, protein efficiency ratio and better nutrient digestibility were recorded for seaweed diet fed fishes. the results suggests the suitability of utilizing seaweeds, ulva fasciata, spyridia ...200415623239
melatonin accelerates maturation inducing hormone (mih): induced oocyte maturation in carps.the present communication is an attempt to demonstrate the influence of melatonin on the action of maturation inducing hormone (mih) on the maturation of oocytes in carps. the oocytes from gravid female major carp labeo rohita were isolated and incubated separately in medium 199 containing (a) only mih (1 microg/ml), (b) only melatonin (at concentrations of 50, 100 or 500 pg/ml), and (c) both melatonin and mih, but at different time intervals. in the latter group, melatonin was added to the incu ...200415639142
toxicity and bioaccumulation of chlorpyrifos in indian carp catla catla (hamilton), labeo rohita (hamilton), and cirrhinus mrigala (hamilton). 200415669740
antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish.motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ...200515747443
dietary yeast rna supplementation reduces mortality by aeromonas hydrophila in rohu (labeo rohita l.) juveniles.a feeding trial was conducted for 60 days to delineate the effect of dietary ribonucleic acid or chitin on haematological parameters, phagocyte respiratory burst and resistance to aeromonas hydrophila of labeo rohita juveniles. one hundred and twenty-six (avg. wt. 13.40 +/- 0.17 g) juveniles were randomly distributed in six treatment groups, each one in three replicates. six isonitrogenous (crude protein: 34.34-35.37%) and isocaloric (414-425 kcal 100 g(-1)) purified diets were prepared with dif ...200515820128
a preliminary report on effect of pulsed magnetic fields on fresh water fish (labeo rohita) chromosomes.marine-life scientists around the world are already carrying out investigative trials to obtain higher yields under in-captive breeding conditions, on both edible varieties and ornamental fishes with optimal inputs. however, for such trials to succeed there is a need for genetic improvement. the idea that fish production can be enhanced by genetic manipulation is gaining acceptance, as there is a strong possibility that qualitative improvement of economically important traits can be achieved by ...200415847344
gill lesions in the major carp, labeo rohita exposed to lethal and sublethal concentrations of tannery effluent.the major carp, labeo rohita were exposed to (0.873%) lethal and sublethal (0.073%) concentrations of tannery effluent for 24h and 40 days respectively under static bioassay condition. the surface architecture of gill revealed severe damages such as : fusion and clumping in the middle and distal parts of the primary lamellae, swelling and deterioration of the cells. the interlamellar space was filled either with hyperplastic epithelial or mucous cells. secondary lamellae lost their identity and ...200415847345
effect of dietary carbohydrate on haematology, respiratory burst activity and histological changes in l. rohita juveniles.a feeding trial was conducted for 60 days to delineate the effect of dietary carbohydrate on the haematology, respiratory burst activity and histology in labeo rohita juveniles. one hundred and forty four fish (av. wt. 10+/-0.15 g) were randomly distributed into 12 treatment groups with each of two replicates. twelve semi-purified diets with either 35% or 28% of crude protein were prepared with different amylase concentrations (0, 50, 100 and 150 mgkg(-1)) and starch type (gelatinised, g/non-gel ...200515863014
effect of sublethal levels of nitrite on some blood parameters of juvenile labeo rohita (hamilton-buchanan).juveniles of l. rohita were exposed to sublethal levels of nitrite (0.02, 0.1 and 0.4 mg/l) for 2, 24, 48 and 96 hr. the time of exposure at individual concentrations of nitrite did not show any significant difference in haemoglobin, cortisol, chloride and lactic acid. haematocrit showed significant reduction with increasing concentration of nitrite irrespective of duration of exposure. fishes exposed to 0.4 mg/l nitrite showed significantly high levels of glucose beyond 2 hr. the mean erythrocy ...200515900911
improvement of nutritive value of grass pea (lathyrus sativus) seed meal in the formulated diets for rohu, labeo rohita (hamilton) fingerlings after fermentation with a fish gut bacterium.eight isonitrogenous (35% crude protein approximately) and isocaloric (4.0 kcalg(-1) approximately) diets were formulated incorporating raw and fermented grass pea (lathyrus sativus) seed meal at 10%, 20%, 30% and 40% levels by weight into a fish meal based diet and fed to rohu, labeo rohita, fingerlings for 80 days and fish performance was studied. a particular bacterial strain (bacillus sp.) isolated from the intestine of adult common carp (cyprinus carpio) reared in the wild having significan ...200515939274
effect of achyranthes aspera on the immunity and survival of labeo rohita infected with aeromonas hydrophila.achyranthes aspera seed was incorporated in the diets (at 0.01%, 0.1% and 0.5%) of labeo rohita, rohu fingerlings (3.0+/-0.4 g). after 2 weeks, the fish were immunized with heat-killed aeromonas hydrophila, and after a further 2 weeks the rohu were experimentally infected with aeromonas hydrophila (atcc 49140). after 7 days blood and serum were sampled to determine superoxide anion production, bactericidal activity, lysozyme, serum protein, albumin, globulin, serum glutamate oxaloacetate transam ...200615961319
evolution and expression of translationally controlled tumour protein (tctp) of fish.translationally controlled tumour protein (tctp) is one of the abundant and ubiquitously expressed proteins in metazoans. in order to better understand its functions in non-mammalians, cdna encoding full-length tctp has been isolated and characterized from a teleost fish, labeo rohita (rohu). encoded by a 1043 nucleotide mrna, rohu tctp consists of 171 amino acids and is expressed in all organs, except in brain. secondary structure of fish tctp mrnas shows that they could be potential substrates ...200516006164
effect of multiple injections of beta-glucan on non-specific immune response and disease resistance in labeo rohita fingerlings.the purpose of this study was to determine if multiple injections of different dosages of beta-glucan derived from barley would enhance the immune response and disease resistance against infections due to opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of beta-glucan suspension in phosphate-buffered saline at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l ...200616039142
the effect of chlorpyrifos, an organophosphate in acetyl cholinesterase activity in freshwater fishes.the freshwater fish catla catla, labeo rohita and cirrhinus mrigala are exposed to the toxicant chlorpyrifos, an organo phosphate commonly used in agriculture as well as in aquaculture. the effect was studied on the enzyme acetyl cholinesterase activity which was inhibited in the selected tissues of the fish. in different tissues and in brain, the maximum inhibition was observed.200516114464
passive transfer of maternal antibodies and their existence in eggs, larvae and fry of indian major carp, labeo rohita (ham.).lack of immune competence in the early stages of life leads to severe mortality in larval stages of different fish species including indian major carp (imc). investigation through indirect enzyme linked immunosorbent assay (elisa) and agglutination test revealed a significant increase in specific serum antibody response in the brood fish of indian major carp, labeo rohita (ham.) following immunisation with a virulent aeromonas hydrophila bacterin 1 month prior to breeding, which was transferred ...200616157486
plasmid-mediated dimethoate degradation by bacillus licheniformis isolated from a fresh water fish labeo rohita.the bacillus licheniformis strain isolated from the intestine of labeo rohita by an enrichment technique showed capability of utilizing dimethoate as the sole source of carbon. the bacterium rapidly utilized dimethoate beyond 0.6 mg/ml and showed prolific growth in a mineral salts medium containing 0.45 mg/ml dimethoate. the isolated b licheniformis exhibited high level of tolerance of dimethoate (3.5 mg/ml) in nutrient broth, while its cured mutant did not tolerate dimethoate beyond 0.45 mg/ml ...200516192686
efficient expression of transgenes in adult zebrafish by electroporation.expression of transgenes in muscle by injection of naked dna is widely practiced. application of electrical pulses at the site of injection was demonstrated to improve transgene expression in muscle tissue. zebrafish is a precious model to investigate developmental biology in vertebrates. in this study we investigated the effect of electroporation on expression of transgenes in 3-6 month old adult zebrafish.200516221312
the immunomodulatory effects of tuftsin on the non-specific immune system of indian major carp, labeo rohita.the purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of tuftsin in pbs suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l. rohita at 2-week intervals for four times. after ...200616293422
assessment of mosquito larvicidal potency of cyclic lipopeptides produced by bacillus subtilis strains.in this study, mosquito larvicidal potency of cyclic lipopeptides (clps) secreted by two bacillus subtilis strains were determined. lc50 of the crude clps secreted by b. subtilis dm-03 and dm-04 strains against third instar larvae of culex quinquefasciatus was 120.0+/-5.0 and 300.0+/-8.0mg/l respectively post 24 h of treatment. physico-chemical factors such as ph of water, incubation temperature, heating and exposure to sunlight hardly influenced the larvicidal potency of these clps. present stu ...200616316617
biochemical changes induced by chlorpyrifos, an organophosphate compound in sublethal concentrations to the freshwater fish catla catla, labeo rohita and cirrhinus mrigala.freshwater fishes, catla catla, labeo rohita and cirrhinus mrigala were exposed to sublethal concentration of the pesticide chlorpyrifos an organophosphate pesticide. biochemical changes in total glycogen, total proteins, lactate dehydrogenase (ldh), alanine amino transferase (alat) and aspartate amino transferase (aat) and nucleic acids, deoxy and ribose, were estimated in the tissues of fishes exposed to the toxicant chlorpyrifos technical grade. the decrease in total glycogen and proteins ove ...200516334264
seasonal ovarian cycle in freshwater teleost, labeo rohita (ham.) in tarai region of uttaranchal.seasonal ovarian cycle, based on histophysiological changes, and correlative variations in liver were studied in an indian major carp, labeo rohita reared in captivity under ambient environmental conditions in tarai region of uttaranchal. the oocyte features used for marking ovarian cycle included nuclear-ooplasmic ratio, yolk vesicle incorporation into ooplasm, number and size of nucleolus and differentiation of follicular layer. based on these features the ovarian cycle in l. rohita was divide ...200516334297
impact of water quality on the stress physiology of cultured labeo rohita (hamilton-buchanan).investigation was conducted for 12 months in two water bodies, s1 with optimum water quality and s2 receiving sewage water. the water quality parameters were assessed in relation to the impact on the stress sensitive physiological parameters of fish labeo rohita. while optimum levels of transparency, dissolved oxygen, unionised ammonia, alkalinity and hardness in s1 reflected in minimum variation of the physiological parameters of l. rohita but suboptimal levels of do (nil-18.0 mg/l) and co2 (ni ...200516334301
influence of sublethal ammonia toxicity on some physiological parameters of labeo rohita (hamilton-buchanan) fingerlings.fingerlings of labeo rohita subjected to sublethal unionized ammonia (0.132mg/l) for 30 days exhibited significant changes. increase in haemoglobin, haematocrit, plasma cortisol, plasma glucose, plasma cholesterol and plasma lactic acid levels whereas, decrease in plasma chloride, liver and muscle glycogen, hepatosomatic index and dna/rna ratio of muscles with stable plasma protein was observed. metabolic recovery was not observed within 30 days of exposure.200516459546
pre- and post-challenge immuno-haematological changes in labeo rohita juveniles fed gelatinised or non-gelatinised carbohydrate with n-3 pufa.the combined effect of dietary carbohydrate type and n-3 pufa (epa+dha) on pre- and post-challenge haemato-immunological responses in labeo rohita juveniles was studied. fish were fed for 67days with six different test diets containing either gelatinised (g) or non-gelatinised (ng) corn (43%) with three levels of n-3 pufa (0.5%, 1.0% and 2.0%). during the pre-challenge period, significantly higher (p<0.05) nbt, serum lysozyme activity, total protein and globulin content was recorded in the ng ca ...200616530425
aflatoxin b1-induced dna damage in labeo rohita: protective effect of an antioxidant supplement, amrita bindu.the present study was undertaken to investigate the effect of potent hepatocarcinogen aflatoxin b1 in adduct formation and dna damage in labeo rohita. also, the salubrious efficacy of an antioxidant supplement amrita bindu (based on indian system of medicine) was investigated. fish weighing 175-250 g were administered intraperitoneally a single dose of 100 microg aflatoxin b1/100 g body wt. and another group was given 20% solution of amrita bindu along with aflatoxin b1 at 100 microg/100 g body ...200616635106
non-specific immune parameters of brood indian major carp labeo rohita and their seasonal variations.different non-specific immune parameters and their seasonal changes in brood indian major carp labeo rohita reared in two major freshwater aquaculture regions of india viz. west bengal and orissa were investigated. it was undertaken for 2 consecutive years and included three main seasons of a year such as summer (march-may), rainy (july-september) and winter (november-january). total serum protein, albumin and globulin levels were not significantly different throughout the year (p>0.01). serum l ...200716679030
acute effects of hexavalent chromium on survival, oxygen consumption, hematological parameters and some biochemical profiles of the indian major carp, labeo rohita.an attempt has been made in the present investigation to determine the acute toxicity of hexavalent chromium and its toxicological effects on survival, physiological, hematological and biochemical parameters of the widely consumed indian major carp, labeo rohita. short-term acute toxicity tests were performed adopting renewal bioassay technique (usepa, 1975) over a period of 96 h, using different concentrations of potassium dichromate to the fish and the 96 h lc[50] value was found to be 111.45 ...200516819101
acute toxicity of mercury to the fingerlings of indian major carps (catla, rohu and mrigal) in relation to water hardness and temperature.in the present study short-term (96 hr) toxicity of mercury in relation to water hardness (270 and 560 mg/l) and temperature (16 degrees c and 35 degrees c) to the fingerlings of indian major carps, i.e. catla, rohu and mrigal has been evaluated using static bioassay. the lc5o indicates that both water hardness and temperature played significant role in mercury toxicity. the test fishes were found most resistant with water hardness of 560 mg/l at 16 degrees c as compared to that of water hardnes ...200616850882
zinc induced histological changes in brain and liver of labeo rohita (ham.).labeo rohita fingerlings were exposed to zinc metal toxicity (5 and 10 ppm) for duration of 5 and 15 days. the histological changes were studied in brain and liver of the treated fish. the brain tissues showed enlarged pyramidal cells with extensive vacoulation while severe necrosis, haemorrhage and degeneration of hepatocytes were witnessed in the liver tissues.200616850886
cloning and expression of an outer membrane protein ompts of aeromonas hydrophila and study of immunogenicity in fish.the outer membrane proteins of the warm water fish pathogen, aeromonas hydrophila have a role in the virulence of the organism and are potential candidates for vaccine development. in this study, the gene encoding an outer membrane protein designated ompts was amplified by pcr excluding the region coding for signal peptide, cloned in pqe 30-ua vector and expressed using induction with isopropyl thiogalactoside (iptg). the size of the expressed protein was 37 kda as estimated by migration in 10% ...200716959494
digestive enzymes and metabolic profile of labeo rohita fingerlings fed diets with different crude protein levels.labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in india. digestive enzyme response and metabolic profile of fingerling l. rohita to different dietary crude protein (cp) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. after 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. amylase, lipase and alkaline phosphatase (alp) activities were not ...200717112756
correlation between biochemical properties and adaptive diversity of skeletal muscle myofibrils and myosin of some air-breathing teleosts.functional properties of myofibrils and relative stability of myosin of five teleosts channa punctata, clarias batrachus, m astacembalus armatus, labeo rohita and catla catla adapted to different breathing modes were compared. myofibrillar contractility and m-atpase of air-breathing organ (abo) possessing c.punctata and c. batrachus were low and least affected by ph in the range of 7.1-8.5. however, their myosin isoforms were relatively thermostable, more soluble at sub-neutral ph values, betwee ...200617133765
effect of mangifera indica kernel as a feed additive on immunity and resistance to aeromonas hydrophila in labeo rohita fingerlings.the study evaluated the efficacy of dietary doses of mangifera indica (mango) kernel on the immune response and disease resistance of labeo rohita fingerlings against the bacterial pathogen aeromonas hydrophila infections in. l. rohita fingerlings fed diet containing 0 (control), 1g, 5 g, 10 g mango kernel kg(-1) dry diet for 60 days. biochemical (serum total protein, albumin, globulin, albumin:globulin ratio, blood glucose), haematological (wbc, rbc, haemoglobin content) and immunological (supe ...200717161956
effects of polyherbal formulation 'immuplus' on immunity and disease resistance of indian major carp, labeo rohita at different stages of growth.a series of experiments were performed to determine the impact of polyherbal immunomodulatory formulation 'immuplus' (aquaimmu) on growth, immunity and disease resistance of rohu (labeo rohita), one of the indian major carp at different stages of growth. rohu larvae were fed on plankton, immuplus-mixed compound feed, and plankton plus immuplus-mixed compound feed (immuplus added at three dose levels of 0.25, 0.50, and 0.75 g/kg feed) from 4th day of hatching to 14th day. immuplus-mixed diets enh ...200717373376
serum antibody response of indian major carp, labeo rohita to three species of pathogenic bacteria; aeromonas hydrophila, edwardsiella tarda and pseudomonas fluorescens.the immune response to mixed whole cell antigens of aeromonas hydrophila, edwardsiella tarda and pseudomonas fluorescens, the common gram negative bacterial pathogens associated with diseases of indian major carps were evaluated for their efficacy in triggering antibody responses in rohu, labeo rohita (ham.). the rohu yearlings were either immunized with antigens from single bacterial strain, a. hydrophila, e. tarda and p. fluorescens or a combination of all three. an antibody response was detec ...200717383016
immunomodulation of labeo rohita juveniles due to dietary gelatinized and non-gelatinized starch.a 60 days experiment was conducted to study the effect of dietary gelatinized (g) and non-gelatinized (ng) starch on immunomodulation of labeo rohita juveniles. two hundred and thirty four juveniles (av. wt. 2.53+/-0.04) were randomly distributed in six treatment groups with each of three replicates. six semi-purified diets containing ng and g corn starch, each at six levels of inclusion (0, 20, 40, 60, 80, 100) were prepared viz., t(1) (100% ng, 0% g starch), t(2) (80% ng, 20% g starch), t(3) ( ...200717383896
changes in the level of transaminases in indian major carp, labeo rohita exposed to sublethal concentration of tannery and distillery effluents.the activity of alanine aminotransferase (alat) and aspartate aminotransferase (aat) of different tissues of fingerlings of labeo rohita under the influence of two effluents has been studied. the alanine aminotransferase activity was increased over the control in different exposed periods of tannery and distillery effluent treatments. the alanine aminotransferase in the liver showed increased activity at different periods than that of the muscle, kidney, gill and brain (p < 0.001) (60.09%) over ...200617402251
effect of dietary supplementation of probiotic and vitamin c on the immune response of indian major carp, labeo rohita (ham.).the immunostimulatory effect of probiotics and vitamin c has been established in many systems including fish. an investigation was carried out to study the effect of dietary supplementation of a probiotic bacterium "bacillus subtilis", vitamin c in the form of ascorbyl polyphosphate and their combination on the immune response of indian major carp, rohu, (labeo rohita ham.) fingerlings fed for a period of 60 days. the total serum protein and globulin content was significantly higher (p<0.05) in ...200717434319
[organization of olfactory system of the indian major carp labeo rohita (ham.): a study using scanning and transmission microscopy].catla catla, labeo rohita, and cirrhinus mrigala are important alimentary fish in india. their reproduction (breeding) depends on season. the fish perceive external factors-stimuli and chemical signals through the olfactory system that plays the key role in the central regulation of reproduction. however, in the available literature, any electron microscopy data on organization of olfactory elements in these fish are absent. we have studied ultrastructure of the olfactory organ in male l. rohita ...200717725034
some quality aspects of fish patties prepared from an indian major carp, labeo rohita (ham.).six different types of fish patties were prepared from de-boned meat of three weight groups (250 500 g, 501-750 g, and 751-1,000 g) of an indian major carp, labeo rohita, using two extenders (boiled potato and corn flour). the weight of the fish and the type of the extender affected the nutritional quality of the patties. cooking lowered the crude protein but increased the total lipid, total soluble sugars, and contents of the patties. cooking yield increased with an increase in the weight of th ...200817852491
effect of arsenic and chromium on the serum amino-transferases activity in indian major carp, labeo rohita.arsenic and hexavalent chromium toxicity results from their ability to interact with sulfahydryl groups of proteins and enzymes, and to substitute phosphorus in a variety of biochemical reactions. alanine aminotransferase (alt; e.c: 2.6.1.2) and aspartate amino transferase (ast; ec 2.6.1.1) play a crucial role in transamination reactions and can be used as potential biomarkers to indicate hepatotoxicity and cellular damage. while histopathological studies in liver tissue require more time and ex ...200717911661
effect of phenol on haematological components of indian major carps catla catla, labeo rohita and cirrhinus mrigala.the effect of phenol on haematological components of indian major carps, catla catla, labeo rohita and cirrhinus mrigala were observed. after exposure to sublethal concentrations of 5.17 mg l(-1), 6.06 mg l(-1) and 6.99 mg l(-1), the number of red blood cells, haemoglobin content and packed cell volume all decreased but the glucose level increased. the order of decrease in the haematological components of the three fish is in the order of catla catla > labeo rohita > cirrhinus mrigala.200717915746
impact of industrial effluents on the biochemical composition of fresh water fish labeo rohita.in acute toxicity (96 hr) experiment the fingerlings of freshwater fish labeo rohita was exposed to tannery, electroplating and textile mill effluents. the lc0 and lc50 concentrations were 15% and 20% for tannery effluents, 3% and 6% for electroplating effluents and 18% and 22% for textile mill effluents respectively. it was found that, electroplating effluent was more toxic than tannery and textile mill wastes. after acute toxicity experiments for different industrial effluents, various tissues ...200717915759
effect of consumption of the nutrient-dense, freshwater small fish amblypharyngodon mola on biochemical indicators of vitamin a status in bangladeshi children: a randomised, controlled study of efficacy.in bangladesh, some commonly consumed, indigenous, freshwater small fish species (eaten whole with bone, head and eyes) such as mola (amblypharyngodon mola) are nutrient-dense, containing preformed vitamin a as retinol and especially 3,4-dehydroretinol. the objective of the present randomised, controlled efficacy study was to evaluate the effects of mola on biochemical indicators of vitamin a status. children (n 196), aged 3-7 years, with serum retinol 0.36-0.75 micromol/l, were randomly allocat ...200817925053
enhanced innate immune parameters in labeo rohita (ham.) following oral administration of bacillus subtilis.the present study assessed the use of bacillus subtilis in fish as a probiotic. the bacterium was administered orally at three different doses 0.5 x 10(7) (t(2)), 1 x 10(7) (t(3)), 1.5 x 10(7) (t(4)) cfu/g feed to labeo rohita for two weeks. the positive control group (t(1)) and negative control group (t(5,)) were fed feed without b. subtilis for the same period. on the 15th day blood and serum were sampled to determine respiratory burst activity (nbt assay), differential leukocyte counts (dlc) ...200818060807
ecotoxicological effects of jute retting on the survival of two freshwater fish and two invertebrates.severe deterioration of water quality occurs during jute retting in ponds, canals, floodplain lakes, and other inland water bodies in the rural areas of west bengal in india. attempts were made to evaluate changes in the physicochemical parameters of water caused by jute retting, and their impact on the survival of two species of freshwater fish (labeo rohita and hypophthalmicthys molitrix) and two species of freshwater invertebrate (daphnia magna, a cladocera, and branchiura sowerbyi, an oligoc ...200818157635
fourier transform infrared spectroscopic characterization and optimization of pb(ii) biosorption by fish (labeo rohita) scales.the present study reports the use of locally available fish (labeo rohita) scales for pb(ii) removal from aqueous solutions under different experimental conditions. maximum pb(ii) adsorption (196.8 mg g(-1)) occurred at ph 3.5. pb(ii) sorption was found to be ph, dose, initial metal concentration, contact time and shaking speed dependent while particle size and temperature independent. experimental data of pb(ii) biosorption onto fish scales fitted well to freundlich isotherm model in comparison ...200818242826
effect of endotoxin on the immunity of indian major carp, labeo rohita.endotoxin, a lipopolysaccharide component of outer cell wall membrane of the gram-negative bacteria is a factor responsible for a number of biological effects including immunostimulatory activities in different animal species including fish. in this study, l. rohita yearlings of weight ranging from 80 to 100g were injected intraperitoneally with 0.5, 1, 2, 5, 10 and 20 eu/fish dose of endotoxin to find out its effect on the immunity. the l. rohita yearlings were found to resist the endotoxin dos ...200818289877
modulation of digestive enzyme activities during ontogeny of labeo rohita larvae fed ascorbic acid enriched zooplankton.the effect of supplementation of ascorbic acid through enriched zooplankton [10%, 20% and 30% ascorbyl palmitate (ap) inclusion in diet of zooplankton] on different digestive enzyme activities during ontogeny of labeo rohita larvae was studied from 4 day to 15 day post hatch. ascorbic acid (aa) content in different groups of unenriched (8.6+/-0.71) and enriched zooplankton were, 750+/-29.3, 1409.1+/-45.5, 2009.21+/-199.2 mug/g respectively on dry matter basis with differences (p<0.05) between th ...200818329933
isolation and characterization of immunoglobulin of the indian major carp, rohu [labeo rohita (ham.)].information on the structure and character of immunoglobulin of fishes is essential in health management. a study was carried out to characterize the serum immunoglobulin (igm) of the indian major carp, rohu labeo rohita (ham.). rohu (500g) were immunised with bovine serum albumin (bsa) and the anti-bsa antibody was purified employing bsa-cl agarose affinity column chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the purified ig in a 3% gel under non-reduce ...200818375144
acid phosphatases from the liver of labeo rohita: purification and characterization.low molecular weight acid phosphatase (lm-acp) peak 2 (the isoenzyme corresponding to isoform 2, if-2) from the liver of fish rahu (labeo rohita) was purified to homogeneity. 900 times purification resulted with specific activity of 35 u/mg of protein and recovery of 0.2%. the enzyme was found homogeneous on sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). molecular weight of 18 killo daltons (kda) was obtained. the peak 1 isoenzyme corresponding to isoform1 (if-1) was part ...200818451497
family association between immune parameters and resistance to aeromonas hydrophila infection in the indian major carp, labeo rohita.seven innate immune parameters were investigated in 64 full-sib families (the offspring of 64 sires and 45 dams) from two year-classes of farmed rohu carp (labeo rohita). survival rates were also available from aeromonas hydrophila infection (aeromoniasis) recorded in controlled challenge tests on a different sample of individuals from the same families. due to strong confounding between the animal additive genetic effect and the family effects (common environmental+non-additive genetic), reliab ...200818486488
prevalence of zoonotic trematodes in fish from a vietnamese fish-farming community.the prevalence of fish-borne zoonotic trematode (fzt) metacercariae was investigated in fish farmed by rural households in nghe an province, located in northern vietnam. in total, 716 fish, including tilapia (oreochromis niloticus) and 6 carp species, i.e., grass carp (ctenopharyngodon idellus), bighead carp (aristichthys nobilis), mrigal (cirrhinus mrigala), common carp (cyprinus carpio), silver carp (hypophthalmychthys molitrix), and roha (labeo rohita), collected from 53 fish farms were exami ...200818564743
gelatinized to non-gelatinized starch ratio in the diet of labeo rohita: effect on digestive and metabolic response and on growth.a 60-days experiment was conducted to study the optimum gelatinized (g) to non-gelatinized (ng) starch ratio in the diet of labeo rohita juveniles with respect to digestive and metabolic response and on growth. two-hundred and thirty-four juveniles (avg. wt 2.53 +/- 0.04 g) were randomly distributed in six treatment groups with each of three replicates. six semipurified diets either containing ng and/or g corn starch viz., t(1) (100% ng, 0% g starch), t(2) (80% ng, 20% g starch), t(3) (60% ng, 4 ...200818662359
use of vitamin c as an immunostimulant. effect on growth, nutritional quality, and immune response of labeo rohita (ham.).teleost fish lack the enzyme for endogenous synthesis of ascorbic acid (aa), an essential micronutrient for fish. the aim of this study was to examine the effect of higher levels of dietary vitamin c on growth, nutritional quality, and immunomodulation in the indian major carp, rohu (labeo rohita). four groups of l. rohita were fed experimental diets containing either no vitamin c (control) or supplemented with vitamin c at 500 mg kg(-1) (exp-1), 1000 mg kg(-1) (exp-2), or 1500 mg kg(-1) (exp-3) ...200818665463
cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in cirrhinus mrigala infected with aeromonas hydrophila.transferrin partial complementary dnas were cloned from the livers of five species in four genera of indian carps (indian major carp species: labeo rohita, catla catla and cirrhinus mrigala; medium carp: puntius sarana; minor carp: labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cdna sequences. the partial transferrin cdnas of the five species of carps had sizes from 624 t ...200918718662
sublethal effects of heavy metals on biochemical composition and their recovery in indian major carps.studies were conducted to assess the effects of sublethal exposure of heavy metals cadmium, arsenic and zinc for 45 days on indian major carps, labeo rohita, cirrhinus mrigala and catla catla. heavy metal treatments in general showed significant reduction in carbohydrate and lipid contents content in muscles as well as in gills in all the three fish species. the order of reduction of muscle and gill carbohydrate and lipid content due to different treatments was cd+as+zn>cd+as>as+zn>cd+zn>cd>as>z ...200918775601
annual reproductive phase-related profile of sex steroids and their carrier, shbg, in the indian major carp, labeo rohita.this study reports annual cyclic changes in the plasma levels of sex hormone binding globulin (shbg), sex steroids (e(2), estradiol; t, testosterone; p, progesterone) and protein (prt) in correlation with reproductive phases in both sexes of an indian major carp, labeo rohita. plasma shbg, e(2), t and prt levels in females and shbg and t levels in males showed steady increases (p<0.005) in parallel with the gonadosomatic index (gsi) escalation in the preparatory and prespawning phases attaining ...200818782576
microbial levan in the diet of labeo rohita hamilton juveniles: effect on non-specific immunity and histopathological changes after challenge with aeromonas hydrophila.a 60-day feeding trial was conducted to study the immuno-protective effect of microbial levan on labeo rohita juveniles challenged with aeromonas hydrophila. six purified diets were prepared with different levels of microbial levan: control (no levan), t1 (basal + 0.25%), t2 (basal + 0.50%), t3 (basal + 0.75%), t4 (basal + 1%) and t5 (basal + 1.25%), fed to six groups of fish in triplicate. among the treatment groups the haemoglobin content and total leucocyte count were increased with a dietary ...200818786027
fourier transform infrared study of protein secondary structural changes in the muscle of labeo rohita due to arsenic intoxication.protein is one of the most important nutrients in human diets and fish has become an increasingly important source of protein in most of the developing countries. among the various fish tissues, muscle is the major contributor of proteins and the whole mass of the body. the goal of this study is to assess the changes in the biochemical compositions in general, and the protein structural changes in particular, in the muscle of labeo rohita fingerlings due to arsenic intoxication using fourier tra ...200818817838
influence of endosulfan and monocrotophos exposure on the activity of nadph cytochrome c reductase (nccr) of labeo rohita (ham).the response of nadph cytochrome c reductase (nccr) activity in liver of labeo rohita fish exposed to the pesticides, 0.25 microgl(-1) endosulfan and 2 mg/l monocrotophos was studied. in terms of specific enzyme activity (mu/mg protein) a significant level of nccr was observed in the liver tissues of labeo rohita exposed to the pesticides, when compared to the control fish (2.460 mu/mg protein). increase of nccr activity was more in the liver of the fish exposed to monocrotophos (4.595 mu/mg pro ...200818831371
the growth hormone-encoding gene isolated and characterized from labeo rohita hamilton is expressed in cho cells under the control of constitutive promoters in 'autotransgene' constructs.the growth hormone (gh) gene along with its regulatory sequences has been isolated from the blood and pituitary gland of labeo rohita. this gh gene is approximately 2.8 kb long and consists of five exons and four introns of varying sizes with ag/ta in its exon-intron junctions. the promoter has a single cyclic amp response unit (cre) element, tata, cat and several pit 1 binding sequences. the 1169-bp gene transcript starts 54 bp upstream of the atg initiation codon and has two polyadenylation si ...200818958599
development of monoclonal antibodies to rohu [labeo rohita] immunoglobulins for use in immunoassays.serum immunoglobulins [ig] of rohu [labeo rohita] were purified by affinity chromatography using bovine serum albumin as capture ligand. the purified rohu ig [r-ig] had a molecular weight [mw] of 880 kda as determined with gel filtration chromatography. the heavy chain of r-ig had an mw of 77.8 kda and that of light chain was 26.4 kda in sds-page. purified r-ig was used for the production of two anti-rohu ig monoclonal antibodies [d7 and h4] that belonged to subclass igg2b and igg1, respectively ...200818996722
metal concentrations in water, sediment, and fish from sewage-fed aquaculture ponds of kolkata, india.the concentrations of lead, cadmium, chromium, copper, and zinc were investigated in the sewage-fed pond water, sediment, and the various organs of labeo rohita, catla catla, cirrhinus mrigala, oreochromis mossambicus, and cyprinus carpio cultured in sewage-fed ponds, kolkata, india. among the metals, cadmium, lead, and zinc were detected in water and, except lead, were below the water quality guideline levels for the protection of freshwater aquatic life proposed by ceqg (canadian environmental ...200918998223
behavioral and respiratory dysfunction in the freshwater fish, labeo rohita (hamilton) under malathion intoxication.a short term definitive test by static renewal bioassay method was conducted to determine the acute toxicity (lc50) of the commercial grade organophosphate insecticide malathion (50% ec) in the freshwater edible fish, labeo rohita. carp fingerlings were exposed for 96 h to different concentrations (6.0 to 10.1 microl/l) of malathion. the acute toxicity value was found to be 9.0 microl/l and one tenth of lc50 (0.9 microl/l) was selected for sub acute studies. behavioral patterns and oxygen consum ...200819024933
non-enzymatic antioxidant status and modulation of lipid peroxidation in the muscles of labeo rohita by sub lethal exposure of cuso4.xenobiotics-mediated environmental stress is an important determining factor in the maintenance of fish health as fishes are frequently exposed to such components. increasing evidences indicate that acute and chronic xenobiotic exposure modulates ros production, suppresses immune response and increase the incidence of fish diseases. in the present context an attempt has been made to study the in vivo effect of different concentrations of cuso(4) (0.5, 1.00 or 2.00 ppm) on lipid peroxidation (an ...200919034682
characterization of igm of indian major carps and their cross-reactivity with anti-fish igm antibodies.indian major carps (imc), rohu (labeo rohita), catla (catla catla) and mrigal (cirrhinus mrigala) were immunized with bovine serum albumin and the serum immunoglobulin m (igm) was purified by affinity chromatography. the heavy and light chain of igm of all the three species of imc were about 88 and 26kda, respectively. anti-fish igm antibody against all the three species were raised in mice and the reaction of anti-fish igm antibodies with igm of all the three species of imc were studied by west ...200919063976
distributions of metals in the food web of fishponds of kolleru lake, india.the distributions of trace metals (pb, cd, cr mn, zn and cu) in water, sediment, plankton and four fish species (labeo rohita, catla catla, pangasius pangasius and cirrhinus mrigala) from fishponds of kolleru lake, india were determined using atomic absorption spectroscopy. the concentrations of lead (0.01-0.03mg/l) and copper (0.01-0.08mg/l) in water and cadmium (3.0-9.0mg/kg), chromium (47-211mg/kg) and copper (10-64mg/kg) in sediment were above the epa threshold effects level (tel) and are of ...200919070365
use of the non-toxic cryoprotectant trehalose enhances recovery and function of fish embryonic stem cells following cryogenic storage.fish embryonic stem (es) cells derived from of blastulae (64 cell stage embryo) of labeo rohita were propagated in culture and retained their es cell-like properties after cryogenic storage (-196 degrees c, i.e., liquid nitrogen). toxic effect of dmso (dimethyl sulphoxide) on stem cells during preservation process has been reported to restrict therapeutic applications. in this study we reduced the concentration of dmso and added the non-toxic cryoprotective agent (cpa) trehalose. cryopreservatio ...200819075757
bioaccumulation of heavy metals in fish tissues of a freshwater lake of bhopal.contamination of heavy metals, namely, lead, cadmium, zinc, nickel, copper, chromium and mercury was evaluated in the samples of water and tissues of labeo rohita and ctenopharyngodon idella of upper lake of bhopal collected during summer, rainy and winter seasons of 2005-2006. different organs of the fishes accumulated varying quantities of different heavy metals. in l. rohita, accumulation of heavy metals was in the sequence liver>kidney>gills>muscles, and in c. idella, it was gills>liver>kidn ...201019123040
non-gelatinized starch influences the deposition of n-3 fatty acids in the muscle of a tropical freshwater fish, labeo rohita.a 60-day feeding trial was conducted to study the influence of gelatinized (g) to non-gelatinized (ng) starch ratio in the diet on fatty acids profiles and oxidative status in labeo rohita fingerlings. two hundred and thirty-four fingerlings (average weight: 2.53 g) were distributed in six treatment groups with each of three replicates. six semi-purified diets either containing ng and/or g corn starch (42.43%) viz., t(1) (100% ng and 0% g starch), t(2) (80% ng and 20% g starch), t(3) (60% ng and ...200919178607
development of 21 new microsatellite markers in labeo rohita (rohu). 200919220228
development and characterization of cell lines derived from rohu, labeo rohita (hamilton), and catla, catla catla (hamilton).two new cell lines, designated re and cb, were derived from the eye of rohu, labeo rohita, and the brain of catla, catla catla, respectively. the cell lines were maintained in leibovitz's l-15 supplemented with 20% foetal bovine serum. the re cell line was sub-cultured for more than 70 passages and the cb cell line for more than 35 passages. the re cells are rounded and consist predominantly of epithelial cells. the cb cell line consists of predominantly fibroblastic-like cells. both cell lines ...200919236559
microbiological quality of finfish and shellfish with special reference to shiga toxin-producing escherichia coli o157.the objective of this study was to determine the microbiological quality of fish and shellfish from kolkata, india, with special emphasis on e. coli o157. fresh and ice-preserved labeo rohita, catla catla, cirrhinus mrigala, oreochromis mossambica, heteropneustesfossilis, clarias batrachus, and penaeus monodon were examined for total heterotrophic bacteria and coliform loads and presence of e. coli and e. coli serotype o157 by culture method. while the total plate count of bacteria was within ac ...200819241559
comparative fatty acid profiles of wild and farmed tropical freshwater fish rohu (labeo rohita).the proximate composition of the whole body and the fatty acid composition of the liver, muscle, eye and brain of wild and cultured rohu (labeo rohita) were analyzed. the cultured species was found to have significantly (p < 0.05) higher lipid contents than its wild counterpart. the saturated (sfa) and monounsaturated (mufa) fatty acid contents were significantly higher in the cultured species, whereas the n-6 and n-3 polyunsaturated fatty acid (pufa) levels were higher in the wild species. fatt ...201019288256
modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and tca cycle of the tropical freshwater fish labeo rohita fed gelatinized and non-gelatinized starch diet.a 60-day experiment was conducted to study the effect of dietary gelatinized (g) and non-gelatinized (ng) starch on the key metabolic enzymes of glycolysis (hexokinase, glucokinase, pyruvate kinase, and lactate dehydrogenase), gluconeogenesis (glucose-6 phosphatase and fructose-1,6 bisphosphatase), protein metabolism (aspartate amino transferase and alanine amino transferase), and tca cycle (malate dehydrogenase) in labeo rohita juveniles. in the analysis, 234 juveniles (2.53 +/- 0.04 g) were ra ...201019340598
modulation of key metabolic enzyme of labeo rohita (hamilton) juvenile: effect of dietary starch type, protein level and exogenous alpha-amylase in the diet.a 60-day feeding trial was conducted to delineate the effect of both gelatinized (g) and non-gelatinized (ng) corn with or without supplementation of exogenous alpha-amylase, either at optimum (35%) or sub-optimum (27%) protein levels, on blood glucose, and the key metabolic enzymes of glycolysis (hexokinase, hk), gluconeogenesis (glucose-6 phosphatase, g6pase and fructose-1,6 bisphosphatase, fbpase), lipogenesis (glucose-6 phosphate dehydrogenase, g6pd) and amino acid metabolism (alanine amino ...200919343525
the effect of euglena viridis on immune response of rohu, labeo rohita (ham.).the study evaluated the effect of dietary doses of euglena viridis on the immune response and disease resistance of labeo rohita fingerlings against infection with the bacterial pathogen aeromonas hydrophila. l. rohita fingerlings were fed with diet containing 0 (control), 0.1 g, 0.5 g, 1.0 g euglena powder kg(-1) dry diet for 90 days. biochemical (serum total protein, albumin, globulin, albumin:globulin ratio), haematological (wbc, rbc, haemoglobin content) and immunological (superoxide anion p ...200919345266
the effect of arsenic exposure and the efficacy of dmsa on the proteins and lipids of the gill tissues of labeo rohita.arsenic is a naturally occurring, highly toxic environmental pollutant. fourier transform infrared (ft-ir) spectroscopy is a non-disturbing technique which provides quantitative information about the molecular composition of biological samples. the aim of this work is to study the compositional and structural changes at the molecular level occurring in gill tissues of labeo rohita fingerlings due to arsenic exposure for various exposure periods by using ft-ir spectroscopic technique. the results ...200919394394
pcr based detection of furadan genotoxicity effects in rohu (labeo rohita) fingerlings.the present study involves the use of rapd-pcr to evaluate the genotoxic effects of furadan in the dna of labeo rohita (rohu) fingerlings. rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. rapd - pcr were carried out with the blood and liver dna samples of both control and treated groups at each of the four sampling hours. a total of six selected rapd primers were used for pcr amplification. template stability has been ...200919466575
alteration in hematology of labeo rohita under stress of pollution from lakes of bangalore, karnataka, india.blood is an indicator of physiological condition of an animal. therefore, a field study was conducted to investigate the hematological parameters of wild population of rohu, labeo rohita (ham). the following aspects were evaluated in blood: hemoglobin content, red blood cell (rbc) and white blood cell (wbc) count, packed cell volume (pcv), and mean corpuscular volume (mcv) and mean corpuscular hemoglobin concentration (mchc) values, and in plasma: cholesterol, protein, and glucose levels. for th ...201019603276
sequence information, ontogeny and tissue-specific expression of complement component c3 in indian major carp, labeo rohita (hamilton).the complement system is one of the first line of immune defence mechanisms as well as a modifier of acquired immunity. c3 is the central complement component primarily synthesized in liver. the local synthesis of c3 in tissues other than liver may play an important role in local inflammatory processes. the present study aims at looking into ontogeny of c3 in labeo rohita and its tissue-specific expression that is yet to be explored for indian carps. unfertilised eggs, and eggs after 0, 1, 3, 6, ...200919775074
gas-liquid chromatography for fenvalerate residue analysis: in vivo alterations in the acetylcholinesterase activity and acetylcholine in different tissues of the fish, labeo rohita (hamilton).the disruption of acetylcholinesterase activity (ache) in the freshwater fish, labeo rohita is demonstrated in the present study using acetylthiocholine iodide as substrate. l. rohita on exposure to lethal (6 microg/l) and sub-lethal (0.75 microg/l) concentrations of fenvalerate showed time- and dose-dependent inhibition in the activity of ache, suggesting a decrease in the cholinergic transmission and consequent accumulation of acetylcholine (ach) in the tissues (brain, gill, liver, and muscle) ...200919778241
parenteral immunization of fish, labeo rohita with poly d, l-lactide-co-glycolic acid (plga) encapsulated antigen microparticles promotes innate and adaptive immune responses.immunogenicity of different antigen preparations of outer membrane proteins (omp) of aeromonas hydrophila such as poly d, l-lactide-co-glycolic acid (plga) microparticles, oil emulsion, neat omp and bacterial whole cells were compared through intra-peritoneal injection in fish, labeo rohita. among these preparations, plga encapsulated antigen stimulated both innate and adaptive immune parameters and the immunogenicity exhibited by plga microparticles was significantly higher (p < 0.05) at both 2 ...201019922799
in situ kinetics of renal β-glucuronidase in teleost, labeo rohita (hamilton).β-glucuronidase is the lysosomal enzyme that increases in the tissues of animals in response to xenobiotics entering the body. kidney is one of the organs where all the xenobiotics can be carried along with the blood for filtration, and therefore, this enzyme may be present in large amount. ours is probably the first attempt in the teleost to study the kinetics of enzyme β-glucuronidase. it has been observed that this enzyme exhibits highest activity at ph 5 in the solution of 0.1 m acetate buff ...201019946795
a study of the subchronic effects of arsenic exposure on the liver tissues of labeo rohita using fourier transform infrared technique.in this work, an attempt has been made to study the subchronic effects of arsenic exposure on the biochemical composition; mainly proteins of the liver tissues of labeo rohita fingerlings by using fourier transform infrared (ft-ir) spectroscopic technique. the study was carried out using a perkin elmer-spectrum rx1 spectrometer. because of arsenic exposure, significant reductions in the intensity as well as area of amide bands have been observed in the liver tissues. the decreased intensity of t ...200920014409
transgene integration - an analysis in autotransgenic labeo rohita hamilton (pisces: cyprinidae).transgenic labeo rohita founder population was analyzed for the presence of autotransgene having histone 3 promoter and growth hormone (gh) cdna (lrh3-ghcdna) or total gh gene (lrh3-gh2.8) by pcr with transgene specific primers. transgene specific amplification was seen with lrh3-ghcdna in five out of seven individuals and all three fishes with lrh3-gh2.8, indicating their transgenic nature. transgene integration was also studied by southern hybridization of dna isolated from blood of the transg ...200520035471
immune responses and expression profiles of some immune-related genes in indian major carp, labeo rohita to edwardsiella tarda infection.edwardsiella tarda is an important gram-negative bacterium that causes systemic infections in a wide range of hosts including fish. the pathogenic mechanisms in this disease are still poorly understood in fish. indian major carp, labeo rohita were intraperitoneally challenged with a pathogenic isolate of e. tarda to measure sequential changes in immunity level. a significant decrease in the superoxide production, myeloperoxidase, alternative complement activity, total protein levels and antiprot ...201020045061
an uncontrolled open pilot study to assess the role of dietary eliminations in reducing the severity of atopic dermatitis in infants and children.the severity of atopic dermatitis (ad) has been reported to be reduced by dietary eliminations in a subset of patients with ad.200920101317
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