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the cultivation and utilization of ramie in the united states of america. 188917788058
x-ray diffraction patterns from plant fibers.the rather long discussion just given seemed necessary in order to establish certain points before attempting to develop the lattice structure and before working out the identity of the structural unit of the ramie fiber. 1. certain planes, 6.10, 5.40, 3.98, etc., as given in table i, run lengthwise of the fiber; that is, they are parallel to the long axis. 2. these planes are in agreement with the assumption that one set, either the 6.10 or the 5.40 is tangential to the fiber and forms concentr ...192519872247
low-angle x-ray scattering from ramie and jute. 195114796753
the microcrystalline structure of cellulose in cell walls of cotton, ramie, and jute fibers as revealed by negative staining of sections.with a new technique of negative staining of sections, it has been possible to observe directly, in ultrathin sections under the electron microscope, the original microcrystalline and microfibrillar structure of cellulose as it occurs in living cells. this method has advantages over the study of isolated fibers used so far by others, in that the original arrangement of microfibrils is better preserved, and their collapse into larger fibrillar units is prevented. with this method, the cell walls ...19664164010
ramie: its potentialities as a vascular prosthetic graft preliminary report of an experimental appraisal. 19665915023
nematode problems affecting agriculture in the philippines.nematodes are considered major pests on most economic crops in the philippines, particularly on banana, pineapple, citrus, tomato, ramie, and sugarcane. radopholus similis is the most destructive nematode on banana, while meloidogyne spp. are more serious on various vegetable crops such as tomato, okra, and celery and on fiber crops such as ramie. tylenchulus semipenetrans is a problem on citrus and rotylenchulus reniformis on pineapple and some legume crops. hirschmanniella oryzae and aphelench ...198819290204
transgenic plants of ramie (boehmeria nivea gaud.) obtained by agrobacterium mediated transformation.a regeneration and transformation protocol for ramie (boehmeria nivea gaud.) is presented. regeneration was obtained from leaf discs placed on solid b-5 medium (gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. co-cultivation of leaf discs with agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by southern blot and analysis of expression of the gus-marker gene.199324201876
ramie (boehmeria nivea) pollen-induced bronchial asthma and allergenic cross-reactivity of ramie and parietaria.ramie (boehmeria nivea), a plant of the urticaceae family, is widely distributed in the nagasaki area, and has been established to be a cause of asthma. the rate of positive reactions to ramie in intradermal tests was 11.7% among adult asthmatic patients in the nagasaki area. in this study, 10 patients were positive in provocation tests using ramie pollen. ramie pollen-specific ige antibodies were measured by elisa, with the positive provocation test group showing higher o.d. values than the pos ...19938323464
miniature crystal models of cellulose polymorphs and other carbohydrates.miniature crystal models of cellulose and other carbohydrates were evaluated with the molecular mechanics program mm3. the models consisted of groups of 24 to 32 monosaccharide residues, with the models of mono- and disaccharides based on well-established, single-crystal work. structures of the cellulose forms and cellotetraose were based on published work using fibre diffraction methods. a structure for the single-chain i alpha cellulose unit cell was also tested. a dielectric constant of about ...19938443130
pectinolytic enzymes from actinomycetes for the degumming of ramie bast fibers.actinomycetes isolated from 10 different soil and compost samples were screened for production of pectinolytic enzyme activities when grown on pectin-containing solid and liquid media. pectinolytic enzymes, detected by using plate diffusion tests with a medium containing ramie (boehmeria nivea) plant material as the sole carbon source, were mainly pectate lyases, but low activities of pectinesterases were also observed. polygalacturonases and polymethylgalacturonases were not produced. multiple ...199416349296
purification and characterization of an extracellular pectate lyase from an amycolata sp.the extracellular pectate lyase (ec 4.2.2.2) of a nonsporulating amycolata sp. was purified to homogeneity by anion- and cation-exchange chromatographies followed by hydrophobic interaction chromatography. the enzyme cleaved polygalacturonate but not highly esterified pectin in a random endolytic transeliminative mechanism that led to the formation of a wide range of 4,5-unsaturated oligogalacturonates. as shown by high-performance anion-exchange chromatography and pulsed amperometric detection, ...19957486993
[chemical constituents of the root of boehmeira nivea (l.) gaud].three compounds were isolated from the root of boehmeria nivea and elucidated according to their spectroscopic data as beta-sitosterol, daucosterol and 19 alpha-hydroxyursolic acid. they are obtained from the herb for the first time.19969642395
ft raman microscopy of untreated natural plant fibres.the application of ft-raman microscopy to the non-destructive analysis of natural plant fibres is demonstrated with samples of flax, jute, ramie, cotton, kapok, sisal and coconut fibre. vibrational assignments are proposed and characteristic features of each material are presented. samples were not pre-treated chemically before analysis and were used directly from their respective storage collection; the adaptation of the raman microscopic technique to the identification of specimens of natural ...19979477578
the antiinflammatory and liver protective effects of boehmeria nivea and b. nivea subsp. nippononivea in rats.the pharmacological effects of boehmeria nivea and b. nivea subsp. nippononivea were studied against carrageenan-induced paw edema, acetaminophen (apap) and d-galactosamine (d-galn) induced hepatotoxicity in rats, respectively. water extracts of b. nivea and b. nivea subsp. nippononivea roots were found to maintain significant antiinflammatory activity against carrageenan-induced edema. moreover, the water extracts of b. nivea and b. nivea subsp. nippononivea significantly decreased the acute in ...199723195578
evaluation of the hepatoprotective and antioxidant activity of boehmeria nivea var. nivea and b. nivea var. tenacissima.in this study, the relationship between liver protective effects and antioxidant activity of boehmeria nivea var. nivea (= b. nivea) and b. nivea var. tenacissima (= b. frutescens) was investigated. the water extracts of both plants exhibited a hepatoprotective activity against ccl4-induced liver injury. b. nivea var. nivea and b. nivea var. tenacissima, also showed anti-oxidant effects in fecl2-ascorbate induced lipid peroxidation in rat liver homogenate. moreover, the active oxygen species sca ...19989533427
[study on the production of beta-mannanase by bacillus m50].bacillus sp. m50 produced beta-mannanase efficiently in the medium composed of 4% konjac powder, 1.0% (nh4)2so4 and 0.35% na2co3. fermentation of beta-mannanase by bacillus sp. m50 has been carried out in 100 l tank. the temperature, ventitation quantity and agitation speed were 30-32 degrees c, 1:0.75 vvm and 220 r/min respectively. the fermentation cycle was 28 h and the highest beta-mannanase activity was 330 u/ml. the optimal temperature and ph for beta-mannanase reaction were 50 degrees c a ...200012548880
enzymatic degumming of ramie bast fibers.bast fibers from ramie (boehmeria nivea) were treated with cell-free culture supernatants from an amycolata sp. and a recombinant streptomyces lividans strain expressing the amycolata pectate lyase to investigate the degumming effects of different extracellular polysaccharide-degrading enzymes. culture supernatants from the amycolata sp. with high pectate lyase activities were most effective in fiber separation and reduced the gum content of ramie fibers by 30% within 15 h. xylanase activity pro ...200010784295
[phytotoxic effect of soil cadmium pollution on ramie].a microplot locating experiment was conducted to study the phytotoxic effect of adding cd in soil on ramie plants. the results showed that the contents of chlorophyll a and b of leaf, and the photosynthesis productivity of ramie plant were significant reduced by the addition of cd. as the content of soil cd reached to 14 mg.kg-1, the biomass yield of the above-ground part of ramie plants was 20% lower than the control, and was 50% lower when soil cd content was at 100 mg.kg-1. the number of effe ...200011767541
[decomposition interaction of mixed litter between chinese fir and various accompanying plant species].studies on the decomposition of mixed litter between chinese fir and 8 accompanying plant species showed that the decomposition of chinese fir litter was promoted to different degrees by 8 mixed plant species, in which angiopteris fokiensis had the greatest effect, while schima superba had certain promotion first, but then, weak inhibition. the order of promotion was angiopteris fokiensis > maesa japonica > ficus simplicissima > woodwardia japonica > boehmeria nivea > castanopsis fargesii > cast ...200111758404
x-ray structure of mercerized cellulose ii at 1 a resolution.a revised crystal structure for mercerized cellulose based on high-resolution synchrotron x-ray data collected from ramie fibers is reported (space group p2(1), a = 8.10(3) a, b = 9.03(3) a, c = 10.31(5) a, gamma = 117.10(5) degrees; 751 reflections in 304 composite spots; theta < 21.11 degrees; lambda = 0.7208 a; lals refinement with d > 1.5 a, r' ' = 0.16; shelx97 refinement with d > 1 a, r = 0.21). as with regenerated cellulose the crystal structure consists of antiparallel chains with differ ...200111749200
degumming of ramie fibers by alkalophilic bacteria and their polysaccharide-degrading enzymes.three strains of alkalophilic bacteria, bacillus sp. nt-39, nt-53 and nt-76, were selected for the degumming of ramie fibers and production of polysaccharide-degrading enzymes. after 48 h of incubation with the strains, the loss of the gum might amount to 5.0% or more of the fibers and a number of polysaccharide-degrading enzymes were secreted to the culture supernatants. the residual gum of the fibers decreased to 9.4% after 5 h of enzymatic degumming. analysis of gum contents and enzyme activi ...200111265793
cp/mas (13)c nmr study of cellulose and cellulose derivatives. 2. complete assignment of the (13)c resonance for the ring carbons of cellulose triacetate polymorphs.complex ring (13)c resonance lines of the cross-polarization/magic angle spinning (cp/mas) (13)c nmr spectra of cellulose triacetate (cta) i and cta ii were completely assigned, for the first time, by (13)c-enriched cta allomorphs. the (13)c-enriched cta i was prepared by heterogeneous acetylation of bacterial cellulose which was biosynthesized by acetobacter xylinum (a. xylinum) atcc10245 from culture medium containing d-(2-(13)c)-, d-(3-(13)c)-, or d-(5-(13)c)glucose as a carbon source, while ...200212071761
inhibition of hepatitis b virus production by boehmeria nivea root extract in hepg2 2.2.15 cells.to explore the anti-hepatitis b virus (hbv) effects of boehmeria nivea (b. nivea) root extract (bne) by using the hepg2 2.2.15 cell model system.200617007029
enzymes in bast fibrous plant processing.the program cost action 847 textile quality and biotechnology (2000-2005) has given an excellent chance to review the possibilities of the research, aiming at development of the industrial application of enzymes for bast fibrous plant degumming and primary processing. the recent advancements in enzymatic processing of bast fibrous plants (flax, hemp, jute, ramie and alike plants) and related textiles are given. the performance of enzymes in degumming, modification of bast fibres, roving, yarn, r ...200616791732
cloning, sequencing and expression of the xylanase gene from a bacillus subtilis strain b10 in escherichia coli.bacillus subtilis strain b10 was isolated for degumming of ramie blast fibers, and a fragment of 642-bp was amplified from chromosomal dna by using primers directed against the sequence of bacillus subtilis xylanase gene given in genbank. the positive clones were screened on the selected lb agar plates supplemented with xylan by congo-red staining method. the recombinant plasmid from one positive clone was used for further analysis and dna sequencing. the gene sequence is different from the repo ...200615951169
a marked enhancement in the production of a highly alkaline and thermostable pectinase by bacillus pumilus dcsr1 in submerged fermentation by using statistical methods.the production of a highly alkaline and thermostable pectinase of bacillus pumilus was optimized in submerged fermentation using plackett-burman design and response surface methodology. three fermentation variables (c:n ratio, k(2)hpo(4), and ph), which were identified to significantly affect pectinase production by plackett-burman design were further optimized using response surface methodology of central composite design (ccd). an over all 34- and 41-fold increase in enzyme production was achi ...200615936940
orientation of native cellulose in an electric field.native cellulose has been oriented in an ac electric field at both the macroscopic and colloidal level. ramie fiber fragments suspended in chloroform have been shown to point along the field. cellulose microcrystal suspensions in cyclohexane have also been allowed to evaporate in an electric field and have exhibited a high degree of orientation when further examined by tem and electron diffraction. similarly, cellulose whisker suspensions showed increasing birefringence with increasing field str ...200616700569
mechanical behavior of cellulose microfibrils in tension wood, in relation with maturation stress generation.a change in cellulose lattice spacing can be detected during the release of wood maturation stress by synchrotron x-ray diffraction experiment. the lattice strain was found to be the same order of magnitude as the macroscopic strain. the fiber repeat distance, 1.033 nm evaluated for tension wood after the release of maturation stress was equal to the conventional wood values, whereas the value before stress release was larger, corresponding to a fiber repeat of 1.035 nm, nearly equal to that of ...200616698777
molecular directionality in cellulose polymorphs.the recently developed technique of reductive amination, followed by gold labeling, was applied to visualize the reducing ends of cellulose microcrystals from cellulose i, cellulose ii, and cellulose iii(i). in these crystals, which were also characterized by electron diffraction, the labeling proved that the chains were organized in a parallel fashion in cellulose i from ramie and valonia and also in cellulose iii(i) from valonia. in microcrystals of cellulose ii from mercerized ramie, the labe ...200616398525
langmuir-blodgett films of cellulose nanocrystals: preparation and characterization.the goal of this work is the preparation of monolayers of cellulose i nanocrystals providing flat crystalline cellulose surfaces. suspensions of cellulose nanocrystals were prepared by hydrolyzing ramie and tunicin fibers with sulfuric acid. due to surface grafted sulfate groups, the negatively charged, rod-like cellulose nanocrystals were found to form stable layers at the air-water interface in the presence of a cationic amphiphilic molecule such as dioctadecyldimethylammonium (doda) used in t ...200717897660
cadmium-induced oxidative stress and response of the ascorbate-glutathione cycle in bechmeria nivea (l.) gaud.bechmeria nivea (l.) gaud. (ramie) seedlings subjected to a series of cadmium (cd) concentrations (0, 1, 3 and 7 mg l(-1)) over a 10d period were investigated with regard to the uptake and distribution of cd, its effect on membrane lipid and chlorophyll synthesis, and response of the ascorbate-glutathione cycle in both leaves and roots. increased cd level in the medium resulted in a significant enhancement of cd concentration in tested plants, with most accumulation in roots. ramie under cd stre ...200717532363
population structure of the classic period maya.this study examines the population structure of classic period (a.d. 250-900) maya populations through analysis of odontometric variation of 827 skeletons from 12 archaeological sites in mexico, guatemala, belize, and honduras. the hypothesis that isolation by distance characterized classic period maya population structure is tested using relethford and blangero's (hum biol 62 (1990) 5-25) approach to r matrix analysis for quantitative traits. these results provide important biological data for ...200717205548
[cloning and expression of atp6 and atp9 genes from ramie (boehmeria nivea (l.) gaud.) and their relationship with cytoplasmic male sterility].the atp6 and apt9 gene fragments associated with cytoplasmic male sterility (cms) were cloned from the mitochondrial dna of a ramie (boehmeria nivea (l.) gaud.) cytoplasmic male sterile line and its maintainer and restorer lines using pcr and degenerated primer strategy. the primers were designed according to the reserved sequences in the encoding region of mitochondrial genes atp6 and atp9 of some dicotyledons from genbank. these fragments did not have complete encoding region but showed the ho ...200819073559
difference of toxicity and accumulation of methylated and inorganic arsenic in arsenic-hyperaccumulating and -hypertolerant plants.the arsenic (as) hyperaccumulators, pteris vittata and pteris cretica and an as-tolerant plant boehmeria nivea, were selected to compare the toxicity, uptake, and transportation of inorganic arsenate (as(v)) and its methylated counterpart dimethylarsinic acid (dma). the xanes method was used to elucidate the effect of as species transformation on as toxicity and accumulation characteristics. significantly higher toxicity and lower accumulation of dmathan inorganic as(v) was shown in the as hyper ...200818754355
highly filled bionanocomposites from functionalized polysaccharide nanocrystals.cellulose and starch nanocrystals obtained from the acid hydrolysis of ramie fibers and waxy maize starch granules, respectively, were subjected to isocyanate-mediated reaction to graft polycaprolactone (pcl) chains with various molecular weights on their surface. grafted nanoparticles were characterized by x-ray diffraction analysis and contact angle measurements. we observed that the nanoparticles kept their initial morphological integrity and native crystallinity. nanocomposite films were pro ...200818510360
[extraction of cd by ramie from soils as affected by applications of chelators and peat].pot experiments were performed to study the effectiveness of chelators (edta, citric acid) and peat in enhancing phyremediation of heavy metal cd by ramie. the results showed that peat increased the ramie's biomass by improving soil's physical and chemical properties, and the relative yields of peat alone, chelators(edta, citric acid) combined with peat were 1.23, 1.13 and 1.41 respectively. so the combination of citric acid and peat was more useful for growth of the ramie. as far as improving c ...200919927838
reinforcing poly(epsilon-caprolactone) nanofibers with cellulose nanocrystals.we studied the use of cellulose nanocrystals (cnxs) obtained after acid hydrolysis of ramie cellulose fibers to reinforce poly(epsilon-caprolactone) (pcl) nanofibers. chemical grafting with low-molecular-weight pcl diol onto the cnxs was carried out in an attempt to improve the interfacial adhesion with the fiber matrix. grafting was confirmed via infrared spectroscopy and thermogravimetric analyses. the polymer matrix consisted of electrospun nanofibers that were collected as nonwoven webs. the ...200920355825
[chemical constituents of roots of boehmeria nivea].to investigate the chemical constituents from roots of boehmeria nivea.200920069903
involvement of grp78 in inhibition of hbv secretion by boehmeria nivea extract in human hepg2 2.2.15 cells.previous studies showed that the root extract of boehmeria nivea (bne) can significantly suppress the production of hepatitis b virus (hbv) in vitro and in vivo. in this study, viral core and large-surface proteins accompanied with their encapsidated viral dna were observed to accumulate within the cells. notably, 78-kda glucose-regulated protein (grp78) was found to be suppressed by bne, and stimulation of the grp78 expression by thapsigargin could rescue virus production initially inhibited by ...200919228285
large-scale degumming of ramie fibre using a newly isolated bacillus pumilus dks1 with high pectate lyase activity.a combined (enzymatic and chemical) process using a bacillus pumilus strain (dks1), isolated from the soil, was used to degum ramie bast fibres. after 24 h of incubation with the isolated pectinolytic strain using a low-cost medium, the weight loss of the ramie fibre was found to be 25% under small scale. high activity of pectate lyase was detected in the culture supernatants; 400 kg of ramie fibres was degummed with 24% weight loss in large-scale degumming under field conditions. no cellulase a ...200918956216
transgenic ramie [boehmeria nivea (l.) gaud.]: factors affecting the efficiency of agrobacterium tumefaciens-mediated transformation and regeneration.in the present study, an efficient agrobacterium-mediated gene transformation system was developed for ramie [boehmeria nivea (l.) gaud.] based on the examinations of several factors affecting plant transformation efficiency. the effects of agrobacterium cell density, acetosyringone, co-cultivation temperature, co-cultivation duration, co-cultivation photoperiod and ph on stable transformation were evaluated. agrobacterium at a concentration of od = 0.5-0.8 improved the efficiency of transformat ...200919533144
degumming and characterization of ramie fibre using pectate lyase from immobilized bacillus pumilus dks1.the present study was aimed at finding the optimal conditions for the production of pectate lyase using immobilized bacillus pumilus dks1 cells in calcium-alginate (ca-alginate) beads and determining the efficient degumming of ramie fibre.200919416461
retraction. transgenic ramie [boehmeria nivea (l.) gaud.]: factors affecting the efficiency of agrobacterium tumefaciens-mediated transformation and regeneration. 200919774385
constitutional tolerance to heavy metals of a fiber crop, ramie (boehmeria nivea), and its potential usage.it is observed that ramie (boehmeria nivea), an economic fiber crop, can establish and colonize metal-contaminated sites in china. metal tolerance and accumulation by ramie originating from 13 metal-contaminated and 4 "clean" sites in china were compared under field and hydroponic conditions. all selected populations and germplasms displayed good growth performance under diverse metal-contaminated habitats; while growth responses, metal accumulation and tolerance were similar among the 8 populat ...201019828218
the effects of boehmeria nivea (l.) gaud. on embryonic development: in vivo and in vitro studies.boehmeria nivea (l.) gaud. was commonly used to treat miscarriages clinically. the aim of this study was to examine its safety for embryonic development.201021195153
molecular characterization of ramie mosaic virus isolates detected in jiangsu and zhejiang provinces, china.virus isolates were obtained from three ramie samples (boehmeria nivea l.) showing yellow mosaic symptoms collected in jiangsu and zhejiang provinces, china. comparison of partial dna-a fragments amplified with begomovirus universal primers pa/pb revealed that these viral isolates shared a high sequence identity. the complete dna-a sequences of two isolates j4 and z1 were determined to be 2736 and 2737 nts, respectively, sharing 94.7% nucleotide sequence identity with each other. also, the dna-b ...201020822317
the anti-hepatitis b virus activity of boehmeria nivea extract in hbv-viremia scid mice.boehmeria nivea extract (bne) is widely used in southern taiwan as a folk medicine for hepato-protection and hepatitis treatment. in previous studies, we demonstrated that bne could reduce the supernatant hepatitis b virus (hbv) dna in hbv-producing hepg2 2.2.15 cells. in the present study, we established an animal model of hbv viremia and used it to validate the efficacy of bne in vivo. in this animal model, serum hbv dna and hbsag were elevated in accordance with tumor growth. to evaluate the ...201018955304
preparation and application of cellulose triacetate microspheres.cellulose triacetate was prepared via reacting of a mixture of acetic anhydride and acetic acid containing sulfuric acid as catalyst with ramie fiber obtained from a biomass of ramie. the cellulose triacetate with a degree of substitution (ds) 2.93 of the ramie fiber was obtained. the honeycomb-like cellulose triacetate microspheres with an average diameter of 14 microm were made from the cellulose triacetate solution. the optimum conditions for preparing the microspheres were determined as cell ...201020060644
mechanical, thermal, and interfacial properties of green composites with ramie fiber and soy resins.fully biodegradable, green composites were fabricated with ramie fibers and modified soy flour (msf) resin. defatted soy flour (sf) was modified by a lab-scale filtration system to improve its mechanical, interfacial, and thermal properties through increasing the protein content. the protein content of sf was increased from 53.1 to 67.5% by filtering out soluble sugars using a microfiber-based filter. tensile stress and young's modulus of msf resins were 35.5 and 1411.7 mpa, respectively, which ...201020405944
evaluation of antiglycosidase and anticholinesterase activities of boehmeria nivea.in this era, major community worldwide is suffering from diabetes type ii, cancer and neurodegenerative disorders. to overcome these diseases, in the screening of korean medicinal plants, we studied the whole plant of boehmeria nivea (b. nivea). the methanolic leaf, stem and root extracts of b. nivea and their respective n-hexane, methylene chloride (ch(2)cl(2)), ethyl acetate (etoac), n-butanol (buoh) and aqueous fractions were investigated for their total phenolic content (tpc), 1,1-diphenyl-2 ...201020363706
isolation of high quality rna and construction of a suppression subtractive hybridization library from ramie (boehmeria nivea l. gaud.).isolation of high quality rna from ramie (boehmeria nivea l. gaud.) is difficult due to its high levels of polyphenols, polysaccharides, pectin, fat, wax and other secondary metabolites. a modified procedure based on guanidinium isothiocyanate for rna preparation of ramie was developed in this study. high concentrations (5%, v/v) of guanidinium isothiocyanate, pvp-4000, sodium citrate and sodium lauryl sarcosinate and beta-mercaptoethanol were used in the extraction buffer, together with a low p ...201019680788
development and validation of molecular markers for characterization of boehmeria nivea var. nivea and boehmeria nivea var. tenacissima.the root of boehmeria spp (ramie) is a hepatoprotective chinese herbal medicine. medicinal properties vary between boehmeria nivea var. nivea and boehmeria nivea var. tenacissima, which are local species found in taiwan. as commercial preparations may use either species, there is a need for a rapid and simple assay to identify variants for quality control.201021114822
[discrimination of bamboo fiber and ramie fiber by near infrared spectroscopy].the research on discrimination of natural bamboo fiber, bamboo pulp fibers and ramie fiber used in textile was demonstrated by near infrared (nir) spectroscopy. first, the spectra of three kinds of fiber were scanned by nir spectrometer. then, the spectral data were pretreated by first derivatives. in the end, the databases and discriminating model of natural bamboo fiber, bamboo pulp fibers and ramie fiber were built. the kind of unknown fiber can be discriminated by the nir model. the results ...201021105396
poly(n-isopropylacrylamide) brushes grafted from cellulose nanocrystals via surface-initiated single-electron transfer living radical polymerization.cellulose nanocrystals (cncs) or nanowhiskers produced from sulfuric acid hydrolysis of ramie fibers were used as substrates for surface chemical functionalization with thermoresponsive macromolecules. the cncs were grafted with poly(n-isopropylacrylamide) brushes via surface-initiated single-electron transfer living radical polymerization (si-set-lrp) under various conditions at room temperature. the grafting process was confirmed via fourier transform ir spectroscopy and x-ray photoelectron sp ...201020843063
[chemical constituents from leaves of boehmeria nivea].to study the chemical constituents from the leaves of boehmeria nivea.201020822015
a procedure for identifying textile bast fibres using microscopy: flax, nettle/ramie, hemp and jute.identifying and distinguishing between natural textile fibres is an important task in both archaeology and criminology. wool, silk and cotton fibres can readily be distinguished from the textile bast fibres flax, nettle/ramie, hemp and jute. distinguishing between the bast fibres is, however, not easily done and methods based on surface characteristics, chemical composition and cross section size and shape are not conclusive. a conclusive method based on x-ray microdiffraction exists, but as the ...201020462699
pyrolysis of ramie residue: kinetic study and fuel gas produced in a cyclone furnace.the thermal decomposition behavior of ramie residue (rr) and the characteristics of fuel gas produced in a cyclone furnace were studied. the pyrolysis kinetics was investigated using thermogravimetric analysis (tga) at heating rates of 5-20°c/min. the results showed that rr mainly decomposed between 250 and 390°c, and the apparent activation energy ranged from 200 to 258 kj/mol. in the cyclone furnace, fast pyrolysis, partial combustion and gasification occurred almost simultaneously, and the th ...201121094601
from interfacial ring-opening polymerization to melt processing of cellulose nanowhisker-filled polylactide-based nanocomposites.in the present work, cellulose nanowhiskers (cnws), extracted from ramie fibers, were incorporated in polylactide (pla)-based composites. prior to the blending, pla chains were chemically grafted on the surface of cnw to enhance the compatibilization between cnw and the hydrophobic polyester matrix. ring-opening polymerization of l-lactide was initiated from the hydroxyl groups available at the cnw surface to yield cnw-g-pla nanohybrids. pla-based nanocomposites were prepared by melt blending to ...201121623629
three new fatty acids from the roots of boehmeria nivea (l.) gaudich and their antifungal activities.three new unsaturated fatty acids, (z)-9,10,11-trihydroxy-12-octadecenoic acid (1), (z)-7,8,9-trihydroxy-10-hexadecenoic acid (2) and (z)-12-keto-7,8,9-trihydroxy-10-hexadecenoic acid (3) were isolated from the roots of boehmeria nivea (l.) gaudich, along with a known unsaturated fatty acid, (e)-8,11,12-trihydroxy-9-octadecenoic acid (4). the structures of the new compounds were established by hr esi-ms, (1)h, (13)c, 2d ((1)h-(1)h cosy, hsqc, hmbc) nmr experiments. the known compound was identif ...201121409726
art, class and gender in joseon dynasty korea: representations of lower-class women by the scholar-painter yun duseo.this paper examines several pioneering genre paintings by the important scholar painter yun duseo (1668-1715), with its focus on their artistic sources which have not yet been explored so far. painted on ramie, 'women picking potherbs' is one of the most intriguing examples among yun duseo's oeuvre, which encompasses a broad variety of themes, including genre imagery, landscapes, portraits, dragons, and horses. even among yun duseo's genre paintings, 'women picking potherbs' is extraordinary, as ...201122171413
lignin variability in plant cell walls: contribution of new models.lignin is a major component of certain plant cell walls. the enzymes and corresponding genes associated with the metabolic pathway leading to the production of this complex phenolic polymer have been studied for many years now and are relatively well characterized. the use of genetically modified model plants (arabidopsis, tobacco, poplar.) and mutants has contributed greatly to our current understanding of this process. the recent utilisation and/or development of a number of dedicated genomic ...201121889043
arg²³⁵ is an essential catalytic residue of bacillus pumilus dks1 pectate lyase to degum ramie fibre.after 24 h of incubation with only purified pectate lyase isolated from bacillus pumilus dks1 (ef467045), the weight loss of the ramie fibre was found to be 25%. to know the catalytic residue of pectate lyase the pel gene encoding a pectate lyase from the strain bacillus pumilus dks1 was cloned in e. coli xl1blue and expressed in e. coli bl21 (de3) plyss. the pel gene was sequenced and showed 1032 bp length. after purification using cm-sepharose the enzyme showed molecular weight of 35 kda and m ...201120596756
biotechnological potential of agro residues for economical production of thermoalkali-stable pectinase by bacillus pumilus dcsr1 by solid-state fermentation and its efficacy in the treatment of ramie fibres.the production of a thermostable and highly alkaline pectinase by bacillus pumilus dcsr1 was optimized in solid-state fermentation (ssf) and the impact of various treatments (chemical, enzymatic, and in combination) on the quality of ramie fibres was investigated. maximum enzyme titer (348.0 ± 11.8 ug(-1) dbb) in ssf was attained, when a mixture of agro-residues (sesame oilseed cake, wheat bran, and citrus pectin, 1 : 1 : 0.01) was moistened with mineral salt solution (a(w) 0.92, ph 9.0) at a su ...201222928091
characterization by suppression subtractive hybridization of transcripts that are differentially expressed in leaves of anthracnose-resistant ramie cultivar.for the purpose of screening putative anthracnose resistance-related genes of ramie (boehmeria nivea l. gaud), a cdna library was constructed by suppression subtractive hybridization using anthracnose-resistant cultivar huazhu no. 4. the cdnas from huazhu no. 4, which were infected with colletotrichum gloeosporioides, were used as the tester and cdnas from uninfected huazhu no. 4 as the driver. sequencing analysis and homology searching showed that these clones represented 132 single genes, whic ...201224415836
prosthetic limb sockets from plant-based composite materials.there is a considerable demand for lower limb prostheses globally due to vascular disease, war, conflict, land mines and natural disasters. conventional composite materials used for prosthetic limb sockets include acrylic resins, glass and carbon fibres, which produce harmful gasses and dust in their manufacture.201222307862
pickering emulsions stabilized by cellulose nanocrystals grafted with thermo-responsive polymer brushes.cellulose nanocrystals (cncs) from ramie fibers are studied as stabilizers of oil-in-water emulsions. the phase behavior of heptane and water systems is studied, and emulsions stabilized by cncs are analyzed by using drop sizing (light scattering) and optical, scanning, and freeze-fracture electron microscopies. water-continuous pickering emulsions are produced with cellulose nanocrystals (0.05-0.5 wt%) grafted with thermo-responsive poly(nipam) brushes (poly(nipam)-g-cncs). they are observed to ...201222204973
optimization of pan bread prepared with ramie powder and preservation of optimized pan bread treated by gamma irradiation during storage.this study was conducted to develop an optimal composite recipe for pan bread with ramie powder that has high sensory approval with all age groups and to estimate the dpph radical scavenging activity and the pan bread shelf life after gamma irradiation. the sensory evaluation results showed significant differences in flavor (p<0.05), appearance (p<0.01), color (p<0.01), moistness (p<0.01), and overall quality (p<0.05) based on the amount of ramie powder added. as a result, the optimum formulatio ...201224471063
easy alignment and effective nucleation activity of ramie fibers in injection-molded poly(lactic acid) biocomposites.the poly(lactic acid) (pla)/ramie fiber biocomposites were fabricated, which exhibited considerable reinforcement effect comparable to the glass fiber at the same loading. the attempts were made to understand the flow-induced morphology of ramie fibers and pla crystals in the injection-molded pla/ramie fiber biocomposites, thus revealing its relationship to biocomposite mechanical properties. the polarized optical microscopy (pom) and two-dimensional wide-angle x-ray diffraction (2d-waxd) were f ...201222806502
polyester-grafted cellulose nanowhiskers: a new approach for tuning the microstructure of immiscible polyester blends.cellulose nanowhiskers (cnw), extracted from ramie fibers by sulfuric acid hydrolysis, were used as substrates to compatibilize binary polyester blends containing 50/50 (w/w) polycaprolactone (pcl) and polylactide (pla). to tailor their interfacial energy and fine-tune their adhesion with the components of the blend, cnw were subjected to different surface polyester grafting by the means of ring-opening polymerization. pcl and pla homopolyesters as well as p(cl-b-la) diblock copolymers were succ ...201222738142
a method for determining reactive hydroxyl groups in natural fibers: application to ramie fiber and its modification.the hydrophilic features of natural fibers (nfs) hinder the widespread application of natural fiber/polymer composites due to the hydroxyl groups (-oh) presented in the cellulose molecule. detecting reactive hydroxyl groups in nfs is very important for optimizing the modification process of natural fibers. this paper proposes a simple and practical methodology to measure reactive hydroxyl groups in nfs using a isocyanate group, a method we term the isocyanate group back titration (ibt) method. a ...201222099251
robot-assisted minimally invasive esophagectomy is equivalent to thoracoscopic minimally invasive esophagectomy.the use of the surgical robot has been increasing in thoracic surgery. its three-dimensional view and instruments with surgical wrists may provide advantages over traditional thoracoscopic techniques. our initial experience with thoracoscopic robot-assisted minimally invasive esophagectomy (ramie) for esophageal cancer was compared with our traditional thoracoscopic minimally invasive esophagectomy (mie) approach for esophageal cancer. a retrospective review of a prospective database was perform ...201221899652
boehmeria nivea attenuates lps-induced inflammatory markers by inhibiting p38 and jnk phosphorylations in raw264.7 macrophages.boehmeria nivea (linn.) gaudich (urticaceae), a natural herb, has a long history of treating several diseases including wound healing. however, the anti-inflammatory effect of b. nivea has not been investigated.201323750815
boehmeria nivea stimulates glucose uptake by activating peroxisome proliferator-activated receptor gamma in c2c12 cells and improves glucose intolerance in mice fed a high-fat diet.we examined the antidiabetic property of boehmeria nivea (l.) gaud. ethanolic extract of boehmeria nivea (l.) gaud. (ebn) increased the uptake of 2-[n-(nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose in c2c12 myotubes. to examine the mechanisms underlying ebn-mediated increase in glucose uptake, we examined the transcriptional activity and expression of peroxisome proliferator-activated receptor gamma (ppar- γ ), a pivotal target for glucose metabolism in c2c12 myotubes. we found that t ...201323690860
construction of flame retardant nanocoating on ramie fabric via layer-by-layer assembly of carbon nanotube and ammonium polyphosphate.a new flame retardant nanocoating has been constructed by the alternate adsorption of polyelectrolyte amino-functionalized multiwall carbon nanotube (mwnt-nh2) and ammonium polyphosphate (app) onto flexible and porous ramie fabric. scanning electron microscopy indicates that the adsorbed carbon nanotube coating is a randomly oriented and overlapped network structure, which is a promising candidate for flame retardancy applications. attenuated total reflection fourier transform infrared spectrosc ...201323459988
de novo assembly and characterization of transcriptome using illumina paired-end sequencing and identification of cesa gene in ramie (boehmeria nivea l. gaud).ramie fiber, extracted from vegetative organ stem bast, is one of the most important natural fibers. understanding the molecular mechanisms of the vegetative growth of the ramie and the formation and development of bast fiber is essential for improving the yield and quality of the ramie fiber. however, only 418 expressed tag sequences (ests) of ramie deposited in public databases are far from sufficient to understand the molecular mechanisms. thus, high-throughput transcriptome sequencing is ess ...201323442184
combined thoracoscopic and laparoscopic robotic-assisted minimally invasive esophagectomy using a four-arm platform: experience, technique and cautions during early procedure development.this study reports an early, single-institution experience of combined thoracoscopic and laparoscopic robotic-assisted minimally invasive esophagectomy (ramie) using a four-arm robotic platform, with special attention given to the pitfalls and complications during procedure development.201323371971
nitrogen removal of ramie stalk treated by acid wastewater combined with clostridium thermocellum and the kinetic study of pyrolysis.a pretreatment was developed to remove nitrogen from ramie residue and cotton stalk to eliminate potential nitrogen pollutants in biomass energy production. in the pretreatment, straw was treated with acid wastewater from bioleaching for 3 h followed by clostridium thermocellum incubation for 2 h. most nitrogen was removed from biomass waste and the major was that in protein. pyrolysis process revealed most hemicellulose was removed and the kinetics fitted the first-order equation well. apparent ...201323220819
polymorphism of cellulose i family: reinvestigation of cellulose ivi.polymorphs of cellulose i, iii(i), and iv(i) have been investigated by x-ray diffraction, ft-ir, and solid-state (13)c nmr spectroscopy. highly crystalline cellulose iii(i) samples were prepared by treating cellulose samples in supercritical ammonia at 140 degrees c for 1 h, and conventional cellulose iii(i) samples were prepared by liquid ammonia treatment. the cellulose iv(i) sample of highest crystallinity was that prepared from cladophora cellulose iii(i) in supercritical ammonia, followed b ...201315244455
periodic disorder along ramie cellulose microfibrils.small angle neutron scattering studies have been carried out on cellulose fibers from ramie and populus maximowicii (cotton wood). labile hydrogen atoms were replaced by deuterium atoms, in water-accessible disordered regions of the fibers, to increase the neutron scattering contrast between the disordered and crystalline regions. a meridional bragg reflection, corresponding to a longitudinal periodicity of 150 nm, was observed when scattering collected from hydrogenated and deuterated dry ramie ...201312857086
an effective degumming enzyme from bacillus sp. y1 and synergistic action of hydrogen peroxide and protease on enzymatic degumming of ramie fibers.enzymatic degumming, as an alternative to chemical processing, has attracted wide attention. however, to date, little information about other enzyme components with effective degumming except pectinase has been reported, and there is no report about the effect of bleaching agent (h2o2) on enzymatic degumming and combining enzymatic degumming and h2o2 bleaching process. in this study, we found that the crude enzyme of wild-type bacillus sp. y1 had a powerful and fast degumming ability. its pgl ac ...201323586022
development and characterization of 1,827 expressed sequence tag-derived simple sequence repeat markers for ramie (boehmeria nivea l. gaud).ramie (boehmeria nivea l. gaud) is one of the most important natural fiber crops, and improvement of fiber yield and quality is the main goal in efforts to breed superior cultivars. however, efforts aimed at enhancing the understanding of ramie genetics and developing more effective breeding strategies have been hampered by the shortage of simple sequence repeat (ssr) markers. in our previous study, we had assembled de novo 43,990 expressed sequence tags (ests). in the present study, we searched ...201323565230
reinforcement of denture base resin with short vegetable fiber.short ramie fibers were selected to investigate the effect of fiber length and volume fraction on the flexural properties of ramie fiber reinforced denture base pmma. with the aid of measured interfacial shear strength and theoretical prediction values, experimental results were well interpreted.201324144826
preparation of activated carbon hollow fibers from ramie at low temperature for electric double-layer capacitor applications.activated carbon hollow fibers (achfs) with high surface area were prepared from inexpensive, renewable ramie fibers (rfs) by a single-step activation method under lower temperature than that of other reports. the effects of activation conditions on the pore structure and turbostratic structure of achfs were investigated systematically. the results show that achfs surface area decreased but micropore volume and conductivity increased as the increase of activation temperature and activation time. ...201324084201
identification of drought stress-responsive transcription factors in ramie (boehmeria nivea l. gaud).ramie fiber extracted from stem bark is one of the most important natural fibers. drought is a main environment stress which severely inhibits the stem growth of ramie and leads to a decrease of the fiber yield. the drought stress-regulatory mechanism of ramie is poorly understood.201324020723
removal of copper from acid wastewater of bioleaching by adsorption onto ramie residue and uptake by trichoderma viride.a continuous batch bioleaching was built to realize the bioleaching of sewage sludge in large scale. in the treatment, heavy metal in acid wastewater of bioleaching was removed by adsorption onto ramie residue. then, acid wastewater was reused in next bioleaching batch. in this way, most time and water of bioleaching was saved and leaching efficiency of copper, lead and chromium kept at a high level in continuous batch bioleaching. it was found that residual heavy metal in sewage sludge is highl ...201323567687
degumming of ramie fiber and the production of reducing sugars from waste peels using nanoparticle supplemented pectate lyase.banana, citrus and potato peels were subjected to treatment with hydroxyapatite nanoparticle (np) supplemented purified pectate lyase (np-pl), isolated from bacillus megaterium ak2 to produce reducing sugar (rs). at both 50 and 90°c production of rs by np-pl was almost twofold greater than that by untreated pectate lyase (pl) from each of the three peels. the optimal production of rs from banana and citrus peels were after 24 and 6h of incubation while it was 24 and 4h for potato peels at 50 and ...201323587821
the alkaline pectate lyase pel168 of bacillus subtilis heterologously expressed in pichia pastoris is more stable and efficient for degumming ramie fiber.the conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. pectate lyases can be used to remove pectin from ramie in a degumming process with reduced environmental pollution and energy consumption. pectate lyase pel168 from bacillus subtilis has been previously characterized and the protein structure was resolved. however, bacillus is not a suitable host for pectate lyases during the degumming process since most bacillus produce ...201323510095
characteristics of polygalacturonate lyase c from bacillus subtilis 7-3-3 and its synergistic action with pela in enzymatic degumming.an alkaline polygalacturonate lyase (pgl) from bacillus subtilis 7-3-3, pelc, with diverse depolymerization abilities for different pectin substrates was found. the pgl activity of pelc decreased with increasing degree of methyl esterification of the substrate. pela and pelc displayed notable synergistic effects in the enzymatic degumming of ramie fibers. gum loss rates increased by 62% when pelc was used to replace up to three-eighths of the pela dose (pelc, 60 u g(-1) ramie fibers). to the bes ...201324236123
enhancing production of alkaline polygalacturonate lyase from bacillus subtilis by fed-batch fermentation.alkaline polygalacturonate lyase (pgl, ec 4.2.2.2) is an enzyme used in many industries. we developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the ph of the fermentative broth to enhance the pgl production from bacillus subtilis 7-3-3 to decrease the production cost. maintaining the fermentation broth at ph 6.5 prior to feeding with ammonia and at ph 6.0 after feeding significantly improved pgl activity (743.5 u ml-1) compare ...201424603713
cloning, expression and characterization of a pectate lyase from paenibacillus sp. 0602 in recombinant escherichia coli.biotechnological applications of microbial pectate lyases (pels) in plant fiber processing are considered as environmentally friendly. as such, they become promising substitutes for conventional chemical degumming process. since applications of pels in various fields are widening, it is necessary to explore new pectolytic microorganisms and enzymes for efficient and effective usage. here, we describe the cloning, expression, characterization and application of the recombinant pel protein from a ...201424612647
anti-hepatitis b virus activity of boehmeria nivea leaf extracts in human hepg2.2.15 cells.boehmeria nivea (linn.) gaudich of the urticaceae family is a perennial ratoon herbal plant, the root of which is used in traditional chinese medicine and possesses a variety of pharmacological properties. the 20% ethanol boehmeria nivea root extract was shown to exert an anti-hepatitis b virus (hbv) effect in vitro and in vivo; however, whether the boehmeria nivea leaf (bnl) extract possesses similar properties has not been determined. in this study, we aimed to investigate the anti-hbv effects ...201424649087
development of a core collection for ramie by heuristic search based on ssr markers.there are more than 2000 ramie germplasms in the national ramie germplasm nursery affiliated with the institute of bast fiber crops, chinese academy of agricultural science, china. as it is difficult to perform effective conservation, management, evaluation, and utilization of redundant genetic resources, it is necessary to construct a core collection by using molecular markers. in this study, a core collection of ramie consisting of 22 germplasms was constructed from 108 accessions by heuristic ...201426019563
enhanced efficiency of cadmium removal by boehmeria nivea (l.) gaud. in the presence of exogenous citric and oxalic acids.boehmeria nivea (l.) gaud. is a potential candidate for the remediation of cd contaminated sites. the present investigation aims to explore cd tolerance threshold and to quickly identify the role of exogenous organic acids in cd uptake and abiotic metal stress damage. elevated cd levels (0-10mg/l) resulted in an obvious rise in cd accumulation, ranging from 268.0 to 374.4 in root and 25.2 to 41.2mg/kg dry weight in shoot, respectively. citric acid at 1.5 mmol/l significantly facilitated cd uptak ...201425499499
genome-wide transcriptional changes of ramie (boehmeria nivea l. gaud) in response to root-lesion nematode infection.ramie fiber extracted from stem bark is one of the most important natural fibers. the root-lesion nematode (rln) pratylenchus coffeae is a major ramie pest and causes large fiber yield losses in china annually. the response mechanism of ramie to rln infection is poorly understood. in this study, we identified genes that are potentially involved in the rln-resistance in ramie using illumina pair-end sequencing in two rln-infected plants (inf1 and inf2) and two control plants (co1 and co2). approx ...201425218245
high-resolution bacterial growth inhibition profiling combined with hplc-hrms-spe-nmr for identification of antibacterial constituents in chinese plants used to treat snakebites.bacterial infection is one of the main secondary infections caused by snakebite. the 88 plant species investigated in this study have been used as folk remedies for treatment of snakebite, and it is therefore the aim of this study to investigate whether the plants contain compounds with bacterial growth inhibition.201425043779
cryphodera sinensis n. sp. (nematoda: heteroderidae), a non-cyst-forming parasitic nematode from the root of ramie boehmeria nivea in china.cryphodera sinensis n. sp. is described from ramie (boehmeria nivea) based on the morphology and molecular analyses of rrna small subunit (ssu), d2d3 expansion domains of large subunit (lsu d2d3) and internal transcribed spacer (its). this new species is characterized by oval females with a distinct subcrystalline layer and pronounced and protruding vulval lip, distinctly concave vulva-anus profile and a vulva-anus distance of 29.5-35.8 μm. males possess two annuli in the lip region, a stylet 27 ...201423782683
comparative proteome analysis of the response of ramie under n, p and k deficiency.ramie is an important natural fiber. there has been little research on the molecular mechanisms of ramie related to the absorption, utilization and metabolism of nitrogen (n), phosphorus (p) and potassium (k). one approach to reveal the mechanisms of n, p and k (npk) utilization and metabolism in ramie is comparative proteome analysis. the differentially expressed proteins in the leaves of ramie were analyzed by proteome analysis after 6 days of n- and k-deficient treatments and 3 days of p-defi ...201424573224
agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [boehmeria nivea (l.) gaud].in this study, leaf midribs, the elite explants, were used for the first time to develop an efficient regeneration and transformation protocol for ramie [boehmeria nivea (l.) gaud.] via agrobacterium-mediated genetic transformation. sensitivity of leaf midribs regeneration to kanamycin was evaluated, which showed that 40 mg l(-1) was the optimal concentration needed to create the necessary selection pressure. factors affecting the ramie transformation efficiency were evaluated, including leaf ag ...201424488319
toward stronger transcrystalline layers in poly(l-lactic acid)/natural fiber biocomposites with the aid of an accelerator of chain mobility.formation of transcrystalline layer probably enhances the interfacial adhesion of poly(l-lactic acid) (plla)/natural fiber biocomposites as confirmed by this work. we found that a crystallization accelerator, poly(ethylene glycol) (peg), improved chain mobility of plla and thus enhanced the growth kinetics of ramie fiber-induced transcrystallinity (tc). the direct observation of polarized optical microscopy during isothermal crystallization revealed that large-sized tc with rapid growth was prod ...201424298881
structural basis for unique hierarchical cylindrites induced by ultrahigh shear gradient in single natural fiber reinforced poly(lactic acid) green composites.a local shear flow field was feasibly generated by pulling the ramie fiber in single fiber reinforced poly(lactic acid) (pla) composites. this was featured by an ultrahigh shear gradient with a maximum shear rate up to 1500 s(-1), a level comparable to that frequently occurring during the practical polymer processing. to distinguish shear-induced self-nucleation and ramie fiber-induced heterogeneous nucleation, the shear history was classified by pulling the fiber for 5 s (pulled sample) and pul ...201424650138
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