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insect-specific irreversible inhibitors of acetylcholinesterase in pests including the bed bug, the eastern yellowjacket, german and american cockroaches, and the confused flour beetle.insecticides directed against acetylcholinesterase (ache) are facing increased resistance among target species as well as increasing concerns for human toxicity. the result has been a resurgence of disease vectors, insects destructive to agriculture, and residential pests. we previously reported a free cysteine (cys) residue at the entrance to the ache active site in some insects but not higher vertebrates. we also reported cys-targeting methanethiosulfonate molecules (amtsn), which, under condi ...201020109441
[bedbug dermatitis (cimex lectularius)]. 201020110070
practical solutions for treating laundry infested with cimex lectularius (hemiptera: cimicidae).the common bed bug, cimex lectularius (l.) (hemiptera: cimicidae) is known to become associated, from time to time, with clothing or linen. in such locations, it may escape insecticide treatment, and may be carried to new locations. we test the suggestion that laundering is sufficient to kill all life stages and thus help prevent reinfestation and dispersion. we establish minimum temperatures for washing and minimum temperatures and times for tumble-drying, as well as testing cold soaking, dry c ...201020214378
characteristics of cimex lectularius (hemiptera: cimicidae), infestation and dispersal in a high-rise apartment building.bed bugs, cimex lectularius l. (hemiptera: cimicidae), are a fast-growing urban pest of significant public health importance in the united states and many other countries. yet, there is very little field research on the ecology of this pest due to its near absence in the united states and most developed nations for several decades. we investigated characteristics of the bed bug infestation and dispersal in a 223-unit high-rise apartment building through visual inspections, intercepting devices, ...201020214383
the comparative toxicity of various contact insecticides to the louse (pediculus humanus l.) and the bed-bug (cimex lectularius l.). 194620276061
insecticides susceptibility status of the bedbugs (cimex lectularius) in a rural area of magugu, northern tanzania.the recent spread of bedbugs, cimex lectularius l. (heteroptera: cimicidae), has received attention of the public health sector for designing of effective plan of action for control. several studies have focused on determining the distribution and abundance of bedbug populations in tropical areas. this study establishes baseline information on deltamethrin, permethrin, alphacypermethrin, lambdacypermethrin and k-o tab susceptibility status in a bedbug population collected from magugu area in nor ...200920300399
widespread distribution of knockdown resistance mutations in the bed bug, cimex lectularius (hemiptera: cimicidae), populations in the united states.we previously reported high deltamethrin resistance in bed bugs, cimex lectularius, collected from multiple areas of the united states (romero et al., 2007). recently, two mutations, the valine to leucine mutation (v419l) and the leucine to isoleucine mutation (l925i) in voltage-gated sodium channel alpha-subunit gene, had been identified to be responsible for knockdown resistance (kdr) to deltamethrin in bed bugs collected from new york (yoon et al., 2008). the current study was undertaken to i ...201020301216
estimating the feeding rate of the bedbug cimex lectularius in an infested room: an inexpensive method and a case study.the common bedbug, cimex lectularius l. (hemiptera: cimicidae), is a globally re-emerging pest that is playing an increasing role in legal disputes and compensation claims as a result of its unpleasant feeding activity. however, there is little information about the feeding frequency of bedbugs outside controlled laboratory cultures. here, we present a simple method of estimating the average time since the last bloodmeal of individual female bedbugs in a single sampling event, applicable to a si ...201020377731
4-oxo-aldehydes from the dorsal abdominal glands of the bed bug (hemiptera: cimicidae).analyses of the dorsal abdominal glands of fourth- and fifth-instar nymphs of the bed bud, cimex lectularius l. (hemiptera: cimicidae), indicated the predominant constituents were (e)-2-hexenal and (e)-2-octenal, with lesser amounts of 4-oxo-(e)-2-hexenal and 4-oxo-(e)-2-octenal. the latter two compounds have not been reported previously as occurring in bed bugs. there were no differences in the chemical composition of the dorsal abdominal glands excised from exuviae left behind by either male o ...201020380293
bed bugs: a public health problem in need of a collaborative solution. 201020420053
insight into the sialome of the bed bug, cimex lectularius.the evolution of insects to a blood diet leads to the development of a saliva that antagonizes their hosts' hemostasis and inflammation. hemostasis and inflammation are redundant processes, and thus a complex salivary potion composed of dozens or near 100 different polypeptides is commonly found by transcriptome or proteome analysis of these organisms. several insect orders or families evolved independently to hematophagy, creating unique salivary potions in the form of novel pharmacological use ...201020441151
circadian rhythm of spontaneous locomotor activity in the bed bug, cimex lectularius l.bed bugs must avoid detection when finding hosts and returning to hidden harborages. their stealthy habits include foraging when hosts are asleep. characteristics of spontaneous locomotor activity rhythm of bed bugs with different feeding histories were studied. in the absence of host stimuli, adults and nymphs were much more active in the dark than in the light. the onset of activity in the scotophase commenced soon after lights-off. the free-running period (tau) for all stages was longer in co ...201020452356
the efficacy of neem seed extracts (tre-san, mitestop on a broad spectrum of pests and parasites.the paper summarizes the acaricidal and insecticidal effects of a patented neem seed extract when diluted 1:10 with shampoo or 1:20, 1:30, 1:33, 1:40, respectively, 1:66 with tap water. it was shown that a broad range of pests and parasites, such as house dust mites, poultry mites, harvest mites, ixodes and rhipicephalus ticks, cat fleas (adults, larvae), bed bugs (all stages), head lice and mallophaga, cockroaches (genera blatta, blattella, gomphadorhina), raptor bugs (triatoma), and even food- ...201020461406
lxxi. the effect of the vapours of various insecticides upon fleas (ceratophyllus fasciatus and xenopsylla cheopis) at each stage in their life history and upon the bed bug (cimex lectularius) in its larval stage. 191420474559
lxxxii. notes on the development of bacillus pestis in bugs (cimex lectularius) and their power to convey infection. 191520474605
some experiments with certain liquid insecticides in houses infested with the bed-bug, cimex lectularius l. 194020475557
the ecology of the bed-bug, cimex lectularius l., in britain: report on research, 1935-40. 194120475600
effects of life stages and feeding regimes on active movement behavior of the tropical bed bug, cimex hemipterus (hemiptera: cimicidae).this study examined the effects of different life stages (first, second, third, fourth, and five instars; adult females and adult males) and feeding regimes (starved and blood fed) on the active movement activity of the tropical bed bug, cimex hemipterus (f.), under mixed-stage conditions. we used an extended arena made from tygon tube coils and observed the movement frequency and movement distance at selected time intervals up to 120 h. the fifth instars and adult males and females showed signi ...201020496576
survival rate of bat bugs (cimex pipistrelli, heteroptera) under different microclimatic conditions.survival of facultative ectoparasites, e.g. bed bugs (cimex spp.), is more intensely affected by climatic factors, namely temperature, than that of permanent ectoparasites. the ontogenetic time of the bat bug (cimex pipistrelli) in bat roosts is limited by different survival rates under different temperatures in particular nymphal stages. this limitation could affect bug densities and cause asynchrony between the ectoparasite and bat reproductive cycle. therefore, bug survival under different te ...201020544221
bed bugs: the pesticide dilemma. 201020556941
the life cycle and effectiveness of insecticides against the bed bugs of thailand.the purpose of this study was to determine the life cycle and effectiveness of insecticides against bed bugs in thailand. bed bugs from dwelling places in chon buri and chiang mai provinces were determined to be cimex hemipterus and cimex lectularius, respectively. both bed bug species were reared by feeding on rabbit blood at 2-day intervals in laboratory conditions at 28-32 degrees c with 75% relative humidity. the duration from egg to adult stages took 39.9 +/- 7.0 and 36.9 +/- 8.2 days in c. ...201020578541
taking a bite out of the bed bug sialome. 201020593899
insecticide-resistance in bed-bugs.a simple method of measuring insecticide-resistance in bed-bugs is described. batches of adult bugs are put on small pieces of filter-paper impregnated with oil solutions of insecticides, and mortality is estimated after 5 days at 25 degrees c.in both cimex lectularius and c. hemipterus, two kinds of resistance can develop, either independently or in conjunction. one involves ddt, methoxychlor and analogues; it cannot be overcome by the addition of dmc. the other involves gamma-bhc, dieldrin and ...195820604028
survey of bed bugs in infested premises in malaysia and singapore.a total of 54 bed bug-infested sites (hotels, public accommodations, and residential premises) in malaysia and singapore was surveyed between july, 2005 and december, 2008. only one species of bed bug was found, the tropical bed bug cimex hemipterus (fabricius). bed bug infestations were common in hotels and public accommodations when compared to residential premises. the three most common locations of infestation within an infested premise were the bedding (31.1%), the headboard (30.3%), and cr ...201020618653
evaluation of chlorfenapyr for control of the bed bug, cimex lectularius l.the presence of bed bug populations resistant to pyrethroids demands the development of new control tactics, including the use of insecticides with new modes of action. insecticides that disrupt oxidative phosphorylation in insect mitochondria can be an option. laboratory assays were used to measure the toxicity of chlorfenapyr to susceptible strains and two strains highly resistant to pyrethroids. the effectiveness of two chlorfenapyr-based formulations was compared, and behavioral responses of ...201020661938
establishment of quantitative sequencing and filter contact vial bioassay for monitoring pyrethroid resistance in the common bed bug, cimex lectularius.two point mutations (v419l and l925i) in the voltage-sensitive sodium channel alpha-subunit gene have been identified in deltamethrin-resistant bed bugs. a quantitative sequencing (qs) protocol was developed to establish a population-based genotyping method as a molecular resistance-monitoring tool based on the frequency of the two mutations. the nucleotide signal ratio at each mutation site was generated from sequencing chromatograms and plotted against the corresponding resistance allele frequ ...201020695274
resurgence of bedbugs in southern france: a local problem or the tip of the iceberg?bedbugs (cimex lectularius) have been feeding on sleeping human beings since prehistory. in europe, bed bugs were common and endemic until world war ii when improved body and home hygiene, and widespread use of insecticides led to almost complete eradication. current evidence indicates that bedbugs are making a comeback in europe, usa, canada and australia. in our practice in southern france, we observed several cases within a period of only 1 year.201120704629
preliminary note on bed-bugs and leprosy. 191320766813
on the multiplication and infectivity of t. cruzi in cimex lectularius. 191420767106
insects and war: ii.-the bed-bug (cimex lectularius). 191420767307
the bed bug: prevention of house infestation. 193320777892
eradication of the bed-bug. 193520778968
naphtha distillates for destruction of bed-bugs. 193720780510
coal-tar naphtha for destruction of bed-bugs. 193820781189
a calcium-activated nucleotidase secreted from ostertagia ostertagi 4th-stage larvae is a member of the novel salivary apyrases present in blood-feeding arthropods.apyrases (atp-diphosphohydrolase) comprise a ubiquitous class of glycosylated nucleotidases that hydrolyse extracellular atp and adp to orthophosphate and amp. one class of newly-described, ca2+-dependent, salivary apyrases known to counteract blood-clotting, has been identified in haematophagous arthropods. herein, we have identified a gene (oos-apy-1) encoding a protein that structurally conforms to the ca2+-activated apyrase from the bed bug, cimex lectularius, by immunologically screening an ...201120809998
arthropod-borne diseases associated with political and social disorder.the living conditions and the crowded situations of the homeless, war refugees, or victims of a natural disaster provide ideal conditions for the spread of lice, fleas, ticks, flies and mites. the consequence of arthropod infestation in these situations is underestimated. along with louse-borne infections such as typhus, trench fever, and relapsing fever, the relationship between acinetobacter spp.-infected lice and bacteremia in the homeless is not clear. murine typhus, tungiasis, and myiasis a ...201120822446
bed bug deterrence.a recent study in bmc biology has determined that the immature stage of the bed bug (the nymph) signals its reproductive status to adult males using pheromones and thus avoids the trauma associated with copulation in this species. the success of this nymphal strategy of deterrence is instructive. against the background of increasing problems with bed bugs, this research raises the question whether pheromones might be used to control them. see research article http://www.biomedcentral.com/1741-70 ...201020828375
nymphs of the common bed bug (cimex lectularius) produce anti-aphrodisiac defence against conspecific males.abdominal wounding by traumatic insemination and the lack of a long distance attraction pheromone set the scene for unusual sexual signalling systems. male bed bugs (cimex lectularius) mount any large, newly fed individual in an attempt to mate. last instar nymphs overlap in size with mature females, which make them a potential target for interested males. however, nymphs lack the female's specific mating adaptations and may be severely injured by the abdominal wounding. we, therefore, hypothesi ...201020828381
eds trying not to let the bed bugs bite.as bed bugs have emerged as a growing problem for eds, managers have developed strategies for prevention and decontamination. here are some of the approaches they have shared with ed management: educate your staff on what they should look for on patients and in rooms that would indicate the presence of bed bugs. be proactive. have an exterminator examine your ed on a regular basis. a decontamination room on the outside of the building will prevent the spread of these bugs in your ed.201020853579
semiochemicals of the common bed bug, cimex lectularius l. (hemiptera: cimicidae), and their potential for use in monitoring and control.the recent resurgence of the common bed bug, cimex lectularius l., has driven an increase in research into the biology and behaviour of this pest. current control is reliant on the application of insecticides, but, owing to the development of insecticide resistance, there is a need for new tools and techniques. semiochemicals (behaviour- and physiology-modifying chemicals) could be exploited for management of bed bugs. the aim of this review was to evaluate studies undertaken in bed bug chemical ...201120859928
the residual toxicity of ddt to bed-bugs. 194521003325
eradication of bed-bugs; a further note. 194521006730
the influence of certain biological factors on the resistance of bed-bugs (cimex lectularius, l.) to ddt. 194621015620
[not available]. 194621017458
acoustic indicators for targeted detection of stored product and urban insect pests by inexpensive infrared, acoustic, and vibrational detection of movement.crawling and scraping activity of three stored-product pests, sitophilus oryzae (l.) (coleoptera: curculionidae), tribolium castaneum (herbst) (coleoptera: tenebrionidae), and stegobium paniceum (l.) (coleoptera: anobiidae), and two urban pests, blattella germanica (l.) (blattodea: blattellidae) and cimex lectularius l. (hemiptera: cimicidae), were monitored individually by infrared sensors, microphones, and a piezoelectric sensor in a small arena to evaluate effects of insect locomotory behavio ...201021061963
uninvited guests: traditional insect repellents in estonia used against the clothes moth tineola bisselliella, human flea pulex irritons and bedbug cimex lectularius.extensive folklore records from pre-modern estonia give us an excellent opportunity to study a variety of local plant knowledge and plant use among the peasantry in various parts of the country. one important biocultural domain where plant knowledge has been crucial was in the various methods of combating different ectoparasites that cohabited and coexisted with humans and their domestic animals. some of these methods were widely known (world-wide, eurasia, europe, baltic rim), while others were ...201021070174
bunnies, bed bugs, and blackberries. 201021133309
the bed bug dilemma. 201021133314
they only come out at night: bed bugs and their alarming resurgence. 201121157285
effects of temperature and humidity on the survival and water loss of cimex hemipterus (hemiptera: cimicidae).the effect of temperature and humidity on the survival and water loss of the tropical bed bug, cimex hemipterus (f.), was studied using two field-collected strains. insects were exposed to temperatures ranging from 20 to 45 degrees c and relative humidities (rhs) of 33, 75, and 100%. c. hemipterus survived longest under the interaction of low temperature (20 degrees c) and high rh (75-100%). survival and water loss were significantly affected (p < 0.01) by temperature and rh (either singly, or i ...201021175045
bedbug infestation and its control practices in gbajimba: a rural settlement in benue state, nigeria.the common bedbug cimex lectularius linnaeus 1758 (hemiptera: cimicidae) is a globally re-emerging pest of serious public health concern. we investigated bedbug infestation in randomly selected apartments in gbajimba community in guma local government area in benue state, nigeria.201021178215
insects and spiders: infestations and bites.despite successful eradication techniques and specific effective therapies, insect bites and infestations remain a source of great human misery. the current scabies pandemic shows no signs of abating. bed bugs, which through the ages have been second only to the malarial mosquito as an insect vector of fatal infection, have now been implicated in the transmission of hepatitis b and possibly african acquired immune deficiency syndrome (aids). the incidence of head- and pubic lice is on the rise, ...198721263961
hematological changes in mice exposed to biting of the bedbug: cimex lectularius l. (hemiptera: cimicidae).the studies on hematologic changes in humans or animals as a result of bedbug bites are lacking. this study was undertaken to examine changes in the blood picture of mice (mus musculus) exposed to cimex lectularius biting. as compared to the check animals, mice exposed to bedbug bites either once or twice within 7 days showed insignificantly higher wbc's (1.6 and 2.8% increase, respectively) and lower hgb content (0.5 and 0.8% decrease, respectively) and significantly higher plt's (p < 0.01) by ...201021268536
transcriptomics of the bed bug (cimex lectularius).bed bugs (cimex lectularius) are blood-feeding insects poised to become one of the major pests in households throughout the united states. resistance of c. lectularius to insecticides/pesticides is one factor thought to be involved in its sudden resurgence. despite its high-impact status, scant knowledge exists at the genomic level for c. lectularius. hence, we subjected the c. lectularius transcriptome to 454 pyrosequencing in order to identify potential genes involved in pesticide resistance.201121283830
bedbugs and infectious diseases.bedbugs are brown and flat hematophagous insects. the 2 cosmopolite species, cimex lectularius and cimex hemipterus, feed on humans and/or domestic animals, and recent outbreaks have been reported in occidental countries. site assessment for bedbug eradication is complex but can be assured, despite emerging insecticide resistance, by hiring a pest-control manager. the common dermatological presentation of bites is an itchy maculopapular wheal. urticarial reactions and anaphylaxis can also occur. ...201121288844
control of cimex lectularius using heat combined with dichlorvos resin strips.successful management of the bed bug, cimex lectularius l. (hemiptera: cimicidae), is difficult because of its pesticide resistance, which can allow a reduction in population, but not elimination. we evaluated the effect of heat and/or air circulation on the efficacy of dichlorvos resin strips in the control of bed bugs. treatments were performed in unoccupied dormitory rooms and consisted of dichlorvos resin strips containing 18.6% active ingredient, the same strips + fan, and strips + fan + he ...201121332764
surface contact toxicity and synergism of several insecticides against different stages of the tropical bed bug, cimex hemipterus (hemiptera: cimicidae).five formulated insecticides (lambda-cyhalothrin at 10 mg m?², bifenthrin at 50 mg m?², fipronil at 10 mg m?², fenitrothion at 50 mg m?², imidacloprid at 5 mg m?²) and one active ingredient (ddt at 500 mg m?²) were evaluated using a surface contact method against early and late instars and adults of two strains of the tropical bed bug, cimex hemipterus (f.). synergism of lambda-cyhalothrin and fipronil using piperonyl butoxide (pbo) was also assessed.201121370390
characterization of an obligate intracellular bacterium in the midgut epithelium of the bulrush bug chilacis typhae (heteroptera, lygaeidae, artheneinae).many members of the suborder heteroptera have symbiotic bacteria, which are usually found extracellularly in specific sacs or tubular outgrowths of the midgut or intracellularly in mycetomes. in this study, we describe the second molecular characterization of a symbiotic bacterium in a monophagous, seed-sucking stink bug of the family lygaeidae (sensu stricto). chilacis typhae possesses at the end of the first section of the midgut a structure which is composed of circularly arranged, strongly e ...201121378044
effectiveness of bed bug monitors for detecting and trapping bed bugs in apartments.bed bugs, cimex lectularius l., are now considered a serious urban pest in the united states. because they are small and difficult to find, there has been strong interest in developing and using monitoring tools to detect bed bugs and evaluate the results of bed bug control efforts. several bed bug monitoring devices were developed recently, but their effectiveness is unknown. we comparatively evaluated three active monitors that contain attractants: cdc3000, nightwatch, and a home-made dry ice ...201121404868
bed bugs revisited. 201121409956
bedbugs (cimicidae infestation): the worldwide renaissance of an old partner of human kind.bedbugs have been known as a human parasite for thousands of years, but scientific studies about this insect are recent and limited. cimex lectularius, the common bedbug, was a well-known parasite in human dwellings until the end of the second world war. nowadays, bedbugs are considered uncommon in the industrialized world. anecdotal reports suggest that bedbugs are getting more common in the united states, canada, and united kingdom. in brazil, there are few reports about bedbug infestations in ...201121412594
alarm pheromones and chemical communication in nymphs of the tropical bed bug cimex hemipterus (hemiptera: cimicidae).the recent resurge of bed bug infestations (cimex spp.; cimicidae) and their resistance to commonly used pesticides calls for alternative methods of control. pheromones play an important role in environmentally sustainable methods for the management of many pest insects and may therefore be applicable for the control of bed bugs. the tropical bed bug, cimex hemipterus, is a temporary ectoparasite on humans and causes severe discomfort. compared to the common bed bug, cimex lectularius, little is ...201121479180
bed bugs, public health, and social justice: part 1, a call to action.the resurgence of bed bugs poses an urgent situation since infestations are rampant globally, nationally, and locally. in ohio, bed bugs have become a virtual epidemic in many towns and cities, especially in central and southwestern regions of the state. these blood-feeding insects cause an array of adverse health effects in humans. furthermore, bed bugs disproportionately occur in urban areas, and housing and the built environment are now recognized as dominant influences on health. bed bugs' p ...201121488465
bed bugs, public health, and social justice: part 2, an opinion survey.bed bug infestations have resurged globally, nationally, and locally, yet the public health community in the u.s. has yet to mount a coordinated response to the escalating bed bug problem. surveys of attendees at the 2009 national environmental health association annual educational conference & exhibition, 2009 ohio association of health commissioners fall conference, 2009 central ohio bed bug summit, and 2010 hamilton county council on aging annual conference were conducted to gauge opinions ab ...201121488466
from the cover: drinking a hot blood meal elicits a protective heat shock response in mosquitoes.the mosquito's body temperature increases dramatically when it takes a blood meal from a warm-blooded, vertebrate host. by using the yellow fever mosquito, aedes aegypti, we demonstrate that this boost in temperature following a blood meal prompts the synthesis of heat shock protein 70 (hsp70). this response, elicited by the temperature of the blood meal, is most robust in the mosquito's midgut. when rna interference is used to suppress expression of hsp70, protein digestion of the blood meal is ...201121518875
resistance differences between chlorpyrifos and synthetic pyrethroids in cimex lectularius population from denmark.bed bug, cimex lectularius l., populations were investigated for resistance against permethrin and chlorpyrifos in a topical application bioassay, after an initial establishment of a discriminating dose with a susceptible population. for both insecticides, ca. two times the lethal dose ld(99) was selected: 2,560 ng of permethrin and 200 ng of chlorpyrifos per bed bug, respectively. bed bugs were collected from infested homes in denmark at ten locations and bred in the laboratory. the frequency o ...201121626157
[renaissance of bed bugs]. 201121644374
bed bugs, public health, and social justice. 201121667722
bedbugs as vectors for drug-resistant bacteria.to the editor: over the past 10 years in north america and western europe, a resurgence of bedbugs (cimex lectularius) has occurred (1). although the basis for this resurgence is unclear, large bedbug infestations have been attributed to increased worldwide travel, altered insecticide management, and increased resistance to pesticides (2). marginalized populations in large urban centers appear to be disproportionately affected; ~30% of shelters in toronto, ontario, canada, report bedbugs (1). we ...201121749792
male mating rate is constrained by seminal fluid availability in bedbugs, cimex lectularius.sexual selection, differences in reproductive success between individuals, continues beyond acquiring a mating partner and affects ejaculate size and composition (sperm competition). sperm and seminal fluid have very different roles in sperm competition but both components encompass production costs for the male. theoretical models predict that males should spend ejaculate components prudently and differently for sperm and seminal fluid but empirical evidence for independent variation of sperm n ...201121779378
bed bugs. 201321830689
bedbugs: an update on recognition and management.the common bedbug (cimex lectularius) is increasingly prevalent and a source of concern and questions for patients. in addition to a range of cutaneous presentations and potential for serious sequelae, bedbug bites cause significant psychological distress and create an economic burden associated with infestation control. recognition of characteristic entomology, clinical presentation, diagnostic features and differential diagnosis can support expedient identification of patients exposed to infes ...201121833462
Multiplex polymerase chain reaction diagnostics of bed bug (Hemiptera: Cimicidae).Cimex lectularius L. (Hemiptera: Cimicidae) is a widespread blood feeding pest of humans around the world, including North America, and has recently undergone a resurgence. A molecular diagnostic technique applying multiplex polymerase chain reaction (PCR) was developed to distinguish bed bug eggs, leg fragments, and degraded samples from other arthropods that frequently occur in human dwellings. A 410-428-bp region of the mitochondrial DNA 16S rRNA gene was used. To design C. lectularius-specif ...201121845958
identification and validation of reference genes for quantitative real-time polymerase chain reaction in cimex lectularius.quantitative real-time polymerase chain reaction (qrt-pcr) has emerged as robust methodology for gene expression studies, but reference genes are crucial for accurate normalization. commonly used reference genes are housekeeping genes that are thought to be nonregulated; however, their expression can be unstable across different experimental conditions. we report the identification and validation of suitable reference genes in the bed bug, cimex lectularius, by using qrt-pcr. the expression stab ...201121845960
Multiple traumatic insemination events reduce the ability of bed bug females to maintain water balance.To examine how traumatic insemination, a wounding process to females inflicted by males during copulation, reduces the longevity of females of the bedbug, Cimex lectularius, we assessed if multiple bouts of mating impact water relations of females by measuring net transpiration water loss rates. Our studies show that net transpiration rate of females correlates with frequency of mating (small increase after exposure to low numbers of males; large increase after exposure to large numbers of males ...201121858473
Insecticide resistance in bedbugs in Thailand and laboratory evaluation of insecticides for the control of Cimex hemipterus and Cimex lectularius (Hemiptera: Cimicidae).Bedbugs are found in many countries around the world, and in some regions they are resistant to numerous insecticides. This study surveyed bedbugs in Thailand and determined their resistance to insecticides. The surveys were carried out in six provinces that attract large numbers of foreign tourists: Bangkok, Chonburi, Chiang Mai, Ubon Ratchathani, Phuket, and Krabi. Bedbugs were collected from hotels and colonized in the laboratory to evaluate their resistance to insecticides. Cimex hemipterus ...201121936321
acute illnesses associated with insecticides used to control bed bugs--seven states, 2003--2010.the common bed bug, cimex lectularius, is a wingless, reddish-brown insect that requires blood meals from humans, other mammals, or birds to survive. bed bugs are not considered to be disease vectors, but they can reduce quality of life by causing anxiety, discomfort, and sleeplessness. bed bug populations and infestations are increasing in the united states and internationally. bed bug infestations often are treated with insecticides, but insecticide resistance is a problem, and excessive use o ...201121937972
deep sequencing of pyrethroid-resistant bed bugs reveals multiple mechanisms of resistance within a single population.a frightening resurgence of bed bug infestations has occurred over the last 10 years in the u.s. and current chemical methods have been inadequate for controlling this pest due to widespread insecticide resistance. little is known about the mechanisms of resistance present in u.s. bed bug populations, making it extremely difficult to develop intelligent strategies for their control. we have identified bed bugs collected in richmond, va which exhibit both kdr-type (l925i) and metabolic resistance ...201122039447
identification and characterization of three cholinesterases from the common bed bug, cimex lectularius.we identified and characterized the full-length cdna sequences encoding two acetylcholinesterases (clache1 and clache2) and a salivary gland-specific cholinesterase-like protein (clsche) from the common bed bug, cimex lectularius. all three cholinesterase genes (clac1, clace2 and clsce) have conserved motifs, including a catalytic triad, a choline-binding site and an acyl pocket. phylogenetic analysis showed that clache1 belongs to the insect ache1 clade, whereas clache2 belongs to the insect ac ...201122136067
human fine body hair enhances ectoparasite detection.although we are relatively naked in comparison with other primates, the human body is covered in a layer of fine hair (vellus and terminal hair) at a relatively high follicular density. there are relatively few explanations for the evolutionary maintenance of this type of human hair. here, we experimentally test the hypothesis that human fine body hair plays a defensive function against ectoparasites (bed bugs). our results show that fine body hair enhances the detection of ectoparasites through ...201122171023
bed bugs can cause severe anaemia in adults.a 60-year-old woman and her 67-year-old male partner, admitted for pneumonia and non-st elevation myocardial infarction, respectively, had severe anaemia (hb 5.3 and 5.2 g/dl, respectively), as a result from massive infestation with cimex lectularius. after two erythrocyte transfusions and thorough decontamination, their clinical course was unremarkable.201122190128
Psychological Effects of Bed Bug Attacks (Cimex lectularius L.).In some individuals, psychological sequelae resulting from bed bug biting events include nightmares, flashbacks, hypervigilance (to keep the bugs away), insomnia, anxiety, avoidance behaviors, and personal dysfunction. These symptoms are suggestive of posttraumatic stress disorder (PTSD).201222195533
psychiatric consequences of actual versus feared and perceived bed bug infestations: a case series examining a current epidemic. 201222221725
rna-seq and molecular docking reveal multi-level pesticide resistance in the bed bug.bed bugs (cimex lectularius) are hematophagous nocturnal parasites of humans that have attained high impact status due to their worldwide resurgence. the sudden and rampant resurgence of c. lectularius has been attributed to numerous factors including frequent international travel, narrower pest management practices, and insecticide resistance.201222226239
bed bugs: clinical relevance and control options.since the late 1990s, bed bugs of the species cimex lectularius and cimex hemipterus have undergone a worldwide resurgence. these bed bugs are blood-sucking insects that readily bite humans. cutaneous reactions may occur and can start out as small macular lesions that can develop into distinctive wheals of around 5 cm in diameter, which are accompanied by intense itching. occasionally, bullous eruptions may result. if bed bugs are numerous, the patient can present with widespread urticaria or ey ...022232375
the re-emergence of the bed bug as a nuisance pest: implications of resistance to the pyrethroid insecticides.a global resurgence of bed bugs (hemiptera: cimicidae) has led to renewed scientific interest in these insects. the current bed bug upsurge appears to have started almost synchronously in the late 1990s in europe, the u.s.a. and australia. several factors have led to this situation, with resistance to applied insecticides making a significant contribution. with a growing number of insecticides (ddt, carbamates, organophosphates etc.) being no longer available as a result of regulatory restrictio ...201222235873
dermatologic infestations.head lice are transmitted by head to head contact. optimal therapy includes malathion lotion 0.5% repeated in one week left on for 30 minutes to 8 hours. spinosad topical suspension 0.9% repeated in one week left on for 10 minutes is another option. scabies is transmitted mainly by direct contact but also via heavily infested fomites due to crusted scabies. permethrin 5% cream to the body repeated in four days is often sufficient; however, scalp treatment with malathion lotion 0.5% is helpful in ...201222250620
smelling your way to food: can bed bugs use our odour?the resurgence in developed countries of the common bed bug, cimex lectularius, has led to a search for new sustainable methods to monitor and control this human ectoparasite. because of increased resistance to insecticides, traps baited with attractive cues are considered a promising method to be developed into efficient monitoring tools for bed bugs. despite their potential as attractants, only a few studies have investigated the odorant cues implicated in the attraction of bed bugs to human h ...201222279069
simultaneous sampling and analysis of indoor air infested with cimex lectularius l. (hemiptera: cimicidae) by solid phase microextraction, thin film microextraction and needle trap device.air in a room infested by cimex lectularius l. (hemiptera: cimicidae) was sampled simultaneously by three different sampling devices including solid phase microextraction (spme) fiber coatings, thin film microextraction (tfme) devices, and needle trap devices (ntds) and then analyzed by gas chromatography-mass spectrometry (gc-ms). the main focus of this study was to fully characterize indoor air by identifying compounds extracted by three different microextraction formats and, therefore, perfor ...201222284870
ige against bed bug (cimex lectularius) allergens is common among adults bitten by bed bugs. 201222305681
rna interference of nadph-cytochrome p450 reductase results in reduced insecticide resistance in the bed bug, cimex lectularius.nadph-cytochrome p450 reductase (cpr) plays a central role in cytochrome p450 action. the genes coding for p450s are not yet fully identified in the bed bug, cimex lectularius. hence, we decided to clone cdna and knockdown the expression of the gene coding for cpr which is suggested to be required for the function of all p450s to determine whether or not p450s are involved in resistance of bed bugs to insecticides.201222347424
mitochondrial dna and morphology show independent evolutionary histories of bedbug cimex lectularius (heteroptera: cimicidae) on bats and humans.the bedbug, cimex lectularius, is a well-known human ectoparasite that is reemerging after a long absence of several decades in developed countries of north america and western europe. bedbugs' original hosts were likely bats, and the bedbugs are still common in their roosts. using morphometry and sequences of mitochondrial cytochrome oxidase subunit i and 16s genes, we showed that the populations on bats and humans are largely isolated and differ in morphology. the character of the morphologica ...201222392133
gaseous chlorine dioxide as an alternative for bedbug control.this study evaluated the efficacy of gaseous chlorine dioxide (clo(2)) for extermination of bedbugs (cimex lectularius and cimex hemipterus).201222476276
a preliminary evaluation of the potential of beauveria bassiana for bed bug control.residual biopesticide treatments of beauveria bassiana were tested against the bed bug cimex lectularius. an oil formulation of conidia was applied to different substrates. bed bugs were exposed for 1 h, transferred to an unsprayed environment and monitored for mortality. separate bioassays evaluated the effect of bed bug strain, sex, life stage, and exposure substrate on mortality. rapid mortality was observed in all bioassays, with bed bugs exposed to treated jersey knit cotton dying most rapi ...201222555012
bedbugs (cimex lectularius): identifying and managing an infestation. 201222635257
molecular markers reveal infestation dynamics of the bed bug (hemiptera: cimicidae) within apartment buildings.the bed bug, cimex lectularius l. (hemiptera: cimicidae), has experienced an extraordinary global resurgence in recent years, the reasons for which remain poorly understood. once considered a pest of lower socioeconomic classes, bed bugs are now found extensively across all residential settings, with widespread infestations established in multiapartment buildings. within such buildings, understanding the population genetic structure and patterns of dispersal may prove critical to the development ...201222679860
when bed bugs bite.treating bed bug bites is straightforward; helping patients control and even prevent future infestations is another matter. here's how you can help.201222754888
ineffectiveness of over-the-counter total-release foggers against the bed bug (heteroptera: cimicidae).field-collected bed bugs (cimex lectularius l.) showed little, if any, adverse effects after 2-h direct exposure to the aerosolized pyrethroid(s) from three over-the-counter total-release foggers ('bug bombs' or 'foggers'); hotshot bedbug and flea fogger, spectracide bug stop indoor fogger, and eliminator indoor fogger. one field-collected population, epm, was an exception in that there was significant mortality at 5-7 d when bugs out in the open had been exposed to the spectracide fogger; morta ...201222812136
effects of juvenile hormone analog formulations on development and reproduction in the bed bug cimex lectularius (hemiptera: cimicidae).bed bugs (cimex lectularius l.) have become a common insect pest in urban areas and are often difficult to manage. eradication is made more problematic by widespread insecticide resistance, raising interest in alternative control products. juvenile hormone analogs (jhas) such as methoprene and hydroprene are relatively harmless to non-arthropods and have proved to be effective against other urban insect pests. two jha products (gentrol(®) and precor(®), central life sciences, schaumburg, il) wer ...201322888044
genetic analysis of bed bug populations reveals small propagule size within individual infestations but high genetic diversity across infestations from the eastern united states.bed bugs (cimex lectularius l.) are a resurgent pest worldwide and infestations within the united states are increasing at a rapid rate. because of the physical and psychological discomfort inflicted by their blood feeding habits, and allergies and secondary infections associated with bites, bed bugs are recognized as a significant public health problem. although bed bug infestations are spreading and becoming more prevalent, we have a poor understanding of their dispersal patterns and sources o ...201222897047
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