Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| evaluation of metastatic cardiac calcification in a model of chronic primary hyperparathyroidism. | recent reports have fueled an interest in the prevalence and significance of metastatic calcium deposition in patients with chronic hyperparathyroidism. experimental data are limited by the lack of suitable in vivo animal models. we have developed a model of marked hypercalcemia and overproduction of parathyroid hormone using somatic gene transfer. briefly, the process involves infection of cultured rodent fibroblasts (rat-1 cells) with a retroviral expression vector that contains the gene encod ... | 1990 | 2247829 |
| structure and oxidation-reduction behavior of 1-deaza-fmn flavodoxins: modulation of redox potentials in flavodoxins. | flavodoxins from clostridium beijerinckii and from megasphaera elsdenii with 1-carba-1-deaza-fmn substituted for fmn have been used to study flavin-protein interactions in flavodoxins. the oxidized 1-deaza analogue of fmn binds to apoflavodoxins from m. elsdenii and c. beijerinckii (a.k.a. clostridium mp) with association constants (ka) of 1.0 x 10(7) m-1 and 3.1 x 10(6) m-1, values about 10(2) less than the corresponding ka values for fmn. x-ray structure analysis of oxidized 1-deaza-fmn flavod ... | 1990 | 2261478 |
| a model of reversible obstructive jaundice in the rat. | a model of reversible, extrahepatic biliary obstruction is described. vessel loop blockade of the biliary tree results in obstructive jaundice while removal of the exteriorized vessel loop provides internal biliary drainage without subsequent laparotomy. this technique combined with a system for chronic venous infusion and arterial blood sampling in the unrestrained rat is ideal for long-term metabolic studies of obstructive jaundice. male fisher 344 rats (275-350 g) underwent either the combine ... | 1990 | 2314093 |
| spike-and-wave neocortical patterns in rats: genetic and aminergic control. | spontaneously occurring and drug-induced high voltage spike-and-wave electroencephalogram patterns were examined in inbred rats of the fischer 344 and buffalo strains and of the random-bred sprague-dawley strain at different ages. in addition, tyrosine hydroxylase activity and dopamine d2 receptor density were determined in the substantia nigra, corpus striatum, olfactory tubercle and ponsmedulla areas of fisher 344 and buffalo animals. high voltage spike-and-wave episodes were present in 87.5% ... | 1990 | 2263319 |
| replication-dependent transactivation of the polyomavirus late promoter. | when a plasmid containing the wild-type polyomavirus intergenic regulatory region fused to the bacterial cat gene was introduced into mouse nih 3t3 cells along with a plasmid coding for the early viral proteins (t antigens), chloramphenicol transacetylase enzyme activity and mrna levels were increased about 10-fold over levels observed in the absence of early proteins. to investigate this transactivation phenomenon further, 11 specific deletion mutant derivatives of the wild-type parent plasmid ... | 1990 | 2154625 |
| analysis of interleukin 2 and various effector cell populations in adoptive immunotherapy of 9l rat gliosarcoma: allogeneic cytotoxic t lymphocytes prevent tumor take. | recombinant interleukin 2 (ril-2) and various effector cell populations were used for adoptive immunotherapy in the fischer strain 9l rat gliosarcoma model. the in vivo cytotoxicities of nonspecifically activated lymphocytes and specifically activated cytotoxic t lymphocytes (ctls) were assessed in a modified in vivo neutralization (winn) assay. effector cells (10(6)) and 9l tumor cells (10(5] were combined with 10(4) units of ril-2 and stereotactically implanted into the right frontal centrum s ... | 1990 | 2263613 |
| [guillain-barré syndrome. 15 pediatric cases]. | clinical features and outcome of 15 children with guillain-barré syndrome were retrospectively analyzed. in 53% of the patients there was an infectious illness previous to the neurologic symptoms and in five children the etiologic agent was demonstrated. motor deficit affecting limbs was the most common clinical presentation and facilal paralysis was the most frequent accompanying sign. one of these children developed the fisher syndrome variant. the outcome was excellent except in one case. we ... | 1990 | 2275491 |
| no difference in expression of chromosomal fragile sites in patients with solid malignant tumours and normal controls. | the frequency and distribution of rare and common chromosomal fragile sites in metaphases derived from peripheral lymphocytes were compared in 26 patients with malignant solid tumours and 24 normal controls. in order to avoid bias in evaluation, the identity of each individual as patient or control was disclosed only after the study was completed. rare heritable folic acid inducible fragile sites were found in five patients (2q13; 6p23; 8q22; 16p12) and two controls (8q22). common fragile sites ... | 1990 | 2278966 |
| spiral ganglion cell endings in the cochlear nucleus of young and old rats. | the spiral ganglion cells (sgcs) forming the auditory nerve have been shown to degenerate with age in both human and animal models, presumably resulting in post-synaptic sites in the cochlear nucleus (cn) that have lost their inputs. the present study examined the morphological changes in the surviving sgc endings in the cn of aged animals. the auditory nerves of 2-3 mo and 25-26 mo male, fisher 344 rats were anterogradely labeled with horseradish peroxidase and the cn prepared histochemically. ... | 1990 | 2292496 |
| effect of aging on intestinal biotin transport in the rat. | the influence of aging of biotin intestinal transport was examined in fisher 344 rats (3- 12- and 24-month old) using a brush border membrane vesicle (bbmv) technique. in all age groups examined, transport of biotin as a function of concentration was saturable in the presence of a na+ gradient (out greater than in), but was lower and linear in the presence of a choline gradient (out greater than in.). the vmax of the biotin transport process was found to be significantly (p less than 0.01) highe ... | 1990 | 2318284 |
| a second thyroglobulin messenger rna species (rtg-2) in rat thyrocytes. | a 0.95-kilobase (kb) thyrocyte rna, initially detected in our 1b-6 subclone of fisher rat thyrocytes (frtl-5) using an oligonucleotide probe complementary to the 5' end of rat thyroglobulin (tg) mrna, was also detected in cultured thyrocytes of the wistar rat (wrt cells) and in freshly isolated normal rat thyroid tissue. this transcript was thyroid specific and as abundant as the previously characterized 9.0-kb tg mrna in the cultured thyroid cells under the growth conditions employed. the small ... | 1990 | 2325666 |
| genitalia in human figure drawings: childrearing practices and child sexual abuse. | to replicate and explore the associations of drawing genitalia on a human figure, child-rearing practices, and a history of alleged sexual abuse, we designed a cross-sectional study of 109 alleged child sexual abuse victims, ages 3 through 8 years, and a group of 109 comparison children matched for age, sex, race, and socioeconomic status but with no history of abuse. a standardized format was used to collect drawings, administer the peabody picture vocabulary test, and gather background data on ... | 1990 | 2329432 |
| [morphological changes in fetal small intestine and pancreas implanted in fisher and august rats]. | fragments of fetal small intestine of fischer rats aged 16-20 days were implanted to rats aged 3 months of the same fischer strain (syngeneic implantation). the morphological changes in the implants were investigated. it has been shown that serious modifications of the implant including the neoplasm formation are possible during the evolution process. after syngeneic implantation of fetal pancreas to fischer and august rats under identical conditions no malignization of the implants was observed ... | 1990 | 2298141 |
| loss of muscarinic m1 receptors with aging in the cerebral cortex of fisher 344 rats. | age-related changes in central cholinergic muscarinic receptors were measured in young (3-6 month), middle-aged (15-17 month), and aged (22-26 month) male fisher 344 rats by receptor binding techniques. using [3h]-quinuclidinyl benzilate as the ligand, a significant decrease (14-19%) in the number of muscarinic cortical receptors was measured in aged rats compared to both young and middle-aged rats. with the selective m1 antagonist, [3h]-pirenzepine, a 17% decrease in receptor density was observ ... | 1990 | 2339151 |
| treatment of humoral hypercalcemia of malignancy in rats with inhibitors of carbonic anhydrase. | the enzyme carbonic anhydrase has been suggested as a critical participant in osteoclast-mediated bone resorption. in humoral hypercalcemia of malignancy (hhm) intense osteoclastic bone resorption is principally responsible for the observed hypercalcemia. we therefore undertook to examine the effect of the carbonic anhydrase inhibitor acetazolamide on the hypercalcemia induced by the h500 leydig cell tumor in fisher rats, a well-described model of hhm. acetazolamide treatment for 10 h at 10 mg/h ... | 1990 | 1964358 |
| hemodynamic effects of 1-[3,4-dihydroxyphenyl]-1,2-diaminoethane versus norepinephrine during ventricular fibrillation and cardiopulmonary resuscitation. | we hypothesized that substitution of the hydroxyl group (oh) on the beta carbon of norepinephrine (ne) with an amino group would yield a compound, 1-(3,4-dihydroxyphenyl)-1,2-diaminoethane (dhpdae), that would maintain the hemodynamic properties of ne during cpr, but would decrease the rate of post-defibrillation dysrhythmias. six mixed breed swine weighing greater than 28 kg were studied. the animals were instrumented for cerebral (cbf) and myocardial blood flow (mbf) measurements. ventricular ... | 1990 | 1965347 |
| aging does not alter muscarinic receptor-mediated inhibition of cyclic amp formation in the striatum and hippocampus. | the effects of aging on the inhibition of forskolin-stimulated cyclic amp formation by muscarinic receptors were investigated. there were no detectable changes in the magnitude of maximal inhibition by carbamylcholine or the potency of the agonist in inducing this response in either the striatum or hippocampus obtained from young or old fisher 344 rats. | 1990 | 1963561 |
| chemical structure and genotoxic activity of the hepatocarcinogenic beta-blocker dl-zami 1305. | a single administration of the sex-dependent hepatocarcinogenic beta-blocker dl-1-(2-nitro-3-methyl-phenoxy)3-tert-butylamino-propan-2-ol (dl-zami 1305) induces liver dna damage, as evaluated by alkaline sucrose gradient analysis, in female but not in male fisher 344 rats. the shift of the methyl-group from the 3-position of the aromatic ring of dl-zami 1305 to the 4- and 5-position causes the progressive decrease of the genotoxic activity of the molecule. no effect on the dna-damaging capacity ... | 1990 | 1967984 |
| cloning of malignantly transformed rat thyroid (frtl) cells with thyrotropin receptors and their growth inhibition by 3',5'-cyclic adenosine monophosphate. | differentiated rat thyroid cells, designated frtl, are totally dependent on tsh for their growth. we continuously cultured frtl cells in the absence of tsh and found that another type of cell appeared in the culture. the new cells were large, flattened and epithelial-like, and none of them exhibited thyroglobulin immunoreactivity. since they grew independently of tsh, we further cloned these mutated cells by the limited dilution method in the absence of tsh. camp production in the cloned cells ( ... | 1990 | 1968381 |
| effect of age and monosodium-l-glutamate (msg) treatment on neurotransmitter content in brain regions from male fischer-344 rats. | peripheral administration of monosodium-l-glutamate (msg) has been found to be neurotoxic in neonatal rats. when administered in an acute, subconvulsive dose (500 mg/kg i.p.), msg altered neurotransmitter content in discrete brain regions of adult (6 month old) and aged (24 month old) male fischer-344 rats. norepinephrine (ne) content was reduced in both the hypothalamus (16%) and cerebellum (11%) of adult rats, but was increased in both the hypothalamus (7%) and cerebellum (14%) of aged rats af ... | 1990 | 1980344 |
| multidisciplinary analysis of patients with extratemporal complex partial seizures. i. intertest agreement. | in 15 patients we hypothesized the origin of epilepsies to be 'extratemporal' based on videotaped seizures and surface eeg. neuropsychological tests and neuroimaging (cat, mri, and pet scans) were then compared to the hypothesized ictal sites. neuropsychological tests were abnormal in 86.6% and fdg-pet scans were abnormal in 73%. the neuropsychological tests and pet localized or lateralized areas of dysfunction to the same sites as electroclinical characteristics did in 85% of patients (p less t ... | 1990 | 2105881 |
| absence of h-ras point mutation at codon 12 in n-methyl-n-nitrosourea-induced hepatocellular neoplasms in the rat. | in order to assess the possibility that activated ras-associated hepatic carcinomas might be much rarer in rats than mice because of the more frequent or rapid occurrence of powerful carcinogenic event(s) other than ras point mutations in the former animals, precancerous lesions and hepatocellular carcinomas induced by a weak hepatocarcinogen n-methyl-n-nitrosourea (mnu) in the rat liver were analyzed for the presence or absence of ras point mutations. mnu was chosen because it is well known tha ... | 1990 | 1975252 |
| the degree of genetic disparity between donor and host correlates with survival of intraventricular substantia nigra grafts. | we have evaluated the degree of genetic disparity that permits survival of intraventricular brain tissue grafts without additional immunosuppression. fisher 344 rats (n = 100) with unilateral substantia nigra lesions were used as hosts. donors consisted of fischer 344 rats (isografts), sprague-dawley rats (allografts), mice, hamsters, and rabbits (xenografts). grafts were obtained from the ventral mesencephalon of embryonic animals. the functional properties of the grafts were measured by behavi ... | 1990 | 1977465 |
| human monoclonal antibodies to pseudomonas aeruginosa type-specific lipopolysaccharides, toxin a and klebsiella capsular polysaccharides. | a number of human monoclonal antibodies (hmab) recognizing type-specific determinants expressed by the lipopolysaccharide (lps) of pseudomonas aeruginosa and by the capsular polysaccharide (cps) of klebsiella were generated for potential treatment of nosocomial infections. the goal is to administer these type-specific hmab prophylactically as a "cocktail" providing broad coverage. lymphoblastoid cell lines (lcl) secreting hmab recognizing p. aeruginosa lps, toxin a or klebsiella cps were obtaine ... | 1990 | 1698165 |
| comparison of growth, neovascularization, and enzymatic function of fetal intestinal grafts in the omentum and renal capsule. | fetal tissues are less immunogenic and may be a useful donor source for organ transplantation. this report compares the fate of fetal small bowel segments transplanted in the omentum and renal capsule of recipient syngeneic rats. two-centimeter segments of fetal jejunum and ileum were obtained from 26 donor 19-day gestational age rat fetuses and transplanted into the subrenal capsule (n = 35) and omentum (n = 40) in syngeneic fisher rats (weight, 150 g) as free grafts. no immunosuppression was u ... | 1990 | 1698219 |
| estrogen increases galanin immunoreactivity in hyperplastic prolactin-secreting cells in fisher 344 rats. | galanin is a widely distributed regulatory peptide which modulates the pituitary secretion of prl and gh. estrogen administration strongly stimulates galanin gene expression in the rat anterior pituitary. in adult female fischer 344 rats, estrogen also induces hyperplasia of lactotropes. we used immunocytochemical analysis to assess the effects of estrogen on galanin-like immunoreactivity (gal-ir) in the rat pituitary and hypothalamus during sc diethylstilbestrol (des) implantation and after its ... | 1990 | 1693569 |
| inhaled particle retention in rats receiving low exposures of diesel exhaust. | to study the effects of a low concentration exposure on the retention and clearance of submicron particles from the lungs, we exposed male fisher 344 rats to diesel exhaust diluted to 50 micrograms diesel exhaust particles (dp)/m3, 20 h/d, 7 d/wk for 52 wk. lung burdens (amount of dp in lungs) and the alveolar macrophage burdens were measured up to 52 wk postexposure. by 1 yr postexposure at least 80% of the dp was eliminated from the lungs and similarly cleared from the lavaged pool of macropha ... | 1990 | 1691304 |
| regional brain monoamine levels and utilization in middle-aged rats. | significant changes in monoamine levels and utilization were noted in certain brain regions of middle-aged fisher 344 rats when compared with young adult controls. in the prefrontal cortex and septum, 3,4 dihydroxyphenylglycol (mhpg) and the mhpg/norepinephrine (ne) ratio were decreased. the septum also showed increases in dopamine (da) and dihydroxyphenylacetic acid (dopac) but there was a decrease in the dopac/da ratio. the striatum showed an increase in the mphg/ne ratio and an increase in do ... | 1990 | 1689446 |
| characterization of flat revertants isolated from cells transformed by abelson murine leukemia virus (ab-mulv). | transformed fisher rat fibroblast cell lines by abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. molecular biological analysis of three revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. these findin ... | 1990 | 1703165 |
| regrowth of damaged neurosecretory axons to fenestrated vessels of implanted peripheral tissues. | a major target of neurosecretory axons (nsa) is the basal lamina around fenestrated blood vessels (fbv) in the neural lobe of the pituitary gland. we have posed the question of whether there is neurovascular specificity. do mature, regenerating nsa terminate selectively on the fbv of the neural lobe compared with the fbv of other tissues that normally are not innervated by nsa? three types of tissue were transplanted between inbred fisher rats. fragments, about 1 mm3, of pineal, adrenal medulla, ... | 1990 | 2343374 |
| root morphology of mandibular premolars in human 45,x females. | the teeth of 87 finnish 45,x females, 60 of their first-degree relatives and 87 normal females were examined. the presence of separate mesial and distal root canals and separate root apices was noted from orthopantomograms. of the 45,x females, 40 (or 46%) had lower premolars with separate root canals; 30 individuals in first and 28 in second premolars. the number of first and second premolars with separate mesial and distal root canals was 40 and 43, and of those also showing separate apices, 2 ... | 1990 | 2344286 |
| proteoglycans synthesized by human glomerular mesangial cells in culture. | human fetal kidney mesangial cells were cultured for 24 h in the presence of 3h-amino acids and [35s] sulfate and chased for 24 h in nonradioactive medium. incubation medium and cell layer proteoglycans were purified twice by high performance liquid chromatography-deae chromatography followed by gel filtration chromatography. the major medium 35s-macromolecules were chondroitin/dermatan-35so4 proteoglycans. a small, sepharose cl-6b kav 0.14 dermatan-35so4 proteoglycan was detected in the labelin ... | 1990 | 2345181 |
| chimeric analysis of the site of lurcher gene action with respect to glial enzyme expression. | immunocytochemical analysis of aggregation chimeras made using either of the mutants, lurcher or purkinje cell degeneration, previously showed that only bergmann glia close to surviving purkinje cells expressed an apparently normal level of the enzyme, glycerol-3-phosphate dehydrogenase (gpdh) (fisher and mullen, 1988). in the present study, aggregation chimeras were made using embryos from a b6d2 hybrid mouse strain carrying the lurcher (lc/+) mutation and homozygous for an allele specifying a ... | 1990 | 2358967 |
| role of calcium in adrenergic neurochemical transmission in the aging heart. | the effect of modification of extracellular ca2+ concentration ([ca2+]0), the administration of the neuronal ca2+ channel blocker, omega-conotoxin, and the calcium (ca2+) ionophore, ionomycin, were examined in the isolated heart of male fisher-344 rats 6 and 24 months of age. hearts with the right cardiac sympathetic nerve intact were isolated and perfused with krebs-ringer solution containing cocaine (10(-6) m) to block uptake 1, metanephrine (10(-6) m) to block uptake 2 and yohimbine (3 x 10(- ... | 1990 | 2359031 |
| pancreatic hepatocytes in fischer and wistar rats induced by repeated injections of cadmium chloride. | the effects of multiple sc injections of cadmium chloride (cdcl2) on the pancreas of two rat strains (wistar [wf/ncr] and fischer [f344/ncr]) were studied by histological and immunohistochemical examinations. a high incidence of hepatocytic foci occurred within the pancreata of both strains that was associated with this cadmium exposure. although pancreatic hepatocytes (ph) were found in both strains, wistar rats were more susceptible to cadmium-induced ph formation while showing more tolerance ... | 1990 | 2360204 |
| analysis of capillary geometry in rat subepicardium and subendocardium. | the sustained high-energy turnover of cardiac muscle presents a formidable challenge to the o2 delivery systems. one major determinant of blood-tissue gas exchange potential is capillary surface area per volume of muscle fiber, sv(c,f). estimation of sv(c,f) necessitates quantification of capillary orientation. capillary geometry was analyzed systematically in subepi- (epi) and subendocardium (endo) of glutaraldehyde perfusion-fixed rat heart (n = 4). on 1-micron sections cut rigorously transver ... | 1990 | 2375407 |
| mutational activation of the cellular harvey ras oncogene in rat esophageal papillomas induced by methylbenzylnitrosamine. | epidemiological studies have demonstrated a strong association between human esophageal cancer and exposure to n-nitroso carcinogens. esophageal tumors can be induced in experimental animals, especially in rats, by many n-nitroso carcinogens. in the present study, rat esophageal tumors induced by methylbenzylnitrosamine (mbna) and mbna-transformed esophageal cell lines were analyzed for activated protooncogenes. dnas from four fisher 344 rat esophageal papillomas were examined for their ability ... | 1990 | 2406014 |
| dose-response relationship between o6-methylguanine formation in clara cells and induction of pulmonary neoplasia in the rat by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. | the relationship between the formation of o6-methylguanine (o6mg) and the induction of lung, liver, and nasal tumors in the fisher 344 rat by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) was examined in a dose-response study. animals were treated for 20 wk (3 times/wk) with concentrations of nnk ranging from 0.03 to 50 mg/kg to induce tumors. steady-state concentrations of o6mg were quantitated, and cytotoxicity was assessed in target cells and tissues af ... | 1990 | 2340522 |
| in vivo dental plaque-forming ability and cariogenicity of the bacterium streptococcus bovis in gnotobiotic rats. | germ-free fisher rats, fed on a high sucrose diet, were monoinfected with streptococcus bovis strains. high levels of fissure caries were formed by three strains; one strain produced moderate levels; another produced only very low levels. no strain could produce approximal or buccal/lingual lesions. all strains could colonize the fissures of teeth in the lower jaw, as shown by scanning electron microscopy. the most cariogenic strains were associated with caries scores similar to those of strep. ... | 1990 | 2344292 |
| exogenous nerve growth factor increases the activity of high-affinity choline uptake and choline acetyltransferase in brain of fisher 344 male rats. | the objective of this study was to determine the effect of age and chronic intracerebral administration of nerve growth factor (ngf) on the activity of the presynaptic cholinergic neuronal markers hemicholinium-sensitive high-affinity choline uptake (hacu) and choline acetyltransferase (chat) in the brain of fisher 344 male rats. in 24-month-old rats, a substantial decrease in chat activity (30%) was measured in striatum, and decreases in hacu were found in frontal cortex (28%) and hippocampus ( ... | 1990 | 2384747 |
| miller fisher syndrome associated with q fever. | a 51 year old woman with pneumonia developed miller fisher syndrome. indirect immunofluorescence tests showed antibodies against coxiella burnetti. miller fisher syndrome associated with q fever has not been described previously. | 1990 | 2391529 |
| reduced high-affinity alpha 1-adrenoceptors in liver of senescent rats: implications of assessment at various temperatures. | 1. we investigated the changes occurring as a result of aging in alpha 1-adrenoceptors in the livers of fisher 344 females rats. for comparison, we also measured beta-adrenoceptors in this tissue. three age groups were studied, including young adults (aged 6 months), mature adults (aged 16 months) and senescent animals (aged 25 months). 2. the density of alpha 1-receptors was measured by use of [3h]-prazosin and was found to be reduced 39% (p less than 0.01) at 25 months compared with 6 months. ... | 1990 | 1963803 |
| evidence for tripeptide-proton symport in renal brush border membrane vesicles. studies in a novel rat strain with a genetic absence of dipeptidyl peptidase iv. | we have investigated the transport characteristics of l-phenylalanyl-l-prolyl-l-alanine in renal brush-border membrane vesicles isolated from japan fisher 344 rats. this particular rat strain genetically lacks dipeptidyl peptidase iv. owing to the absence of this enzyme, the tripeptide was found to be completely resistant to hydrolysis by the renal brush-border membrane vesicles. uptake of the tripeptide into these membrane vesicles in the presence of an inwardly directed na+ gradient was slight ... | 1990 | 1967607 |
| immunocytochemical localization of proteoglycans within normal elastin fibers. | appreciable amounts of glycosaminoglycans have been found by immunocytochemistry within mature elastin fibers of human dermis. on thin sections, elastin fibers showed antigenic sites for monoclonal antibodies recognizing the unsaturated units remaining after digestion of hyaluronic acid with streptomyces hyaluronidase and after digestion of dermatan and chondroitin sulfates with chondroitinase abc. moreover, sectioned elastin fibers were positive towards antibodies raised against synthetic pepti ... | 1990 | 2127920 |
| evaluation of heartworm immunodiagnostic tests. | in this report, the use of appropriate statistical methods for the evaluation of heartworm immunodiagnostic tests is discussed. the evaluation of these tests is complicated by factors causing variation in sensitivity, specificity, accuracy, and predictive values of positive and negative test results. the primary sources of inconsistency are variation in the prevalence of heartworm infection among populations of dogs and the sensitivity of immunodiagnostic tests to various categories of heartworm ... | 1990 | 2211321 |
| substrate alterations in a sarcoma-bearing rat model: effect of tumor growth and resection. | the time course of plasma substrate level alterations in the tumor-bearing (tb) host and their relationship to tumor burden or weight loss has not been well defined. plasma glucose, lactate, pyruvate, cholesterol, triglycerides, acetoacetate, beta-hydroxy butyrate, and glycerol levels were measured in postabsorptive tb and nontumor-bearing (ntb) male fisher 344 rats at weekly intervals for 5 weeks. chronically starved rts (cs) had plasma substrates measured at week 3 and compared with tb and ntb ... | 1990 | 2352423 |
| alveolar epithelial cell injuries by subchronic exposure to low concentrations of ozone correlate with cumulative exposure. | electron microscopy morphometry has been used to study the effects of cumulative exposure of low levels of inhaled o3 on lung proximal alveolar tissue. six-week-old fisher 344 rats were exposed to o3 in two different subchronic low-level exposure patterns. the first was a 12 hr/day exposure for 6 weeks and included two o3 concentrations, 0.12 and 0.25 ppm. the second consisted of an exposure profile having a background level of 0.06 ppm with an exposure peak 5 days each week that went from 0.12 ... | 1991 | 2068723 |
| response of serum zinc and copper to inflammatory stimulation in the rat with chronic liver damage. | to measure the seric levels of zinc and copper in healthy and in hepatic damaged rats when an inflammatory stimulus is applied. | 1991 | 1844115 |
| strain differences in expression of virulence by the 90 kilobase pair virulence plasmid of salmonella serovar typhimurium. | a number of plasmidless strains were obtained by curing the 90 kilobase pair (kb) virulence plasmid from six strains, c5, tml, w118, sr11, lt2 and fisher, of salmonella serovar typhimurium. a number of transposon (tn5) tagged 90 kb plasmids, also derived from these typhimurium strains, were then transferred back into these plasmidless strains. plasmid-cured strains, reconstituted strains, and the parental strains were tested for their virulence in balb/c mice. there were two groups of typhimuriu ... | 1991 | 1654496 |
| hepatitis b virus infection markers in genetic haemochromatosis. a study of 272 patients. | serum hepatitis b virus (hbv) infection markers were studied in 272 patients with homozygous genetic haemochromatosis complicated (n = 33) or not (n = 239) with primary liver cancer (plc). controls consisted of 255 029 healthy blood donors from whom age- and sex-matched control groups were extracted for statistical evaluation using the fisher exact test. in blood donors, hbsag was positive in 0.075% of males and 0.04% of females. this population was not screened for anti-hbs. anti-hbc alone (wit ... | 1991 | 1667016 |
| metabolism and disposition of p-chloroaniline in rat, mouse, and monkey. | the metabolism and disposition of [14c]p-chloroaniline was studied in the male fisher 344 rat, female c3h mouse, and male rhesus monkey. greater than 90% of the radiocarbon is eliminated through the urine in all species. a major route of metabolism is orthohydroxylation, whereby 2-amino-5-chlorophenyl sulfate is the major excretion product in all species. excretion of p-chloro-oxanilic acid and p-chloroglycolanilide, oxidation products of p-chloroacetanilide, is significant in the rat. the appea ... | 1991 | 1676638 |
| influence of subepithelial deposits on permeability of the glomerular capillary wall in serum sickness nephritis in the rat. | serum sickness nephritis was induced in male fisher rats by immunization with egg albumin (ea). correlations of subepithelial deposits (sd) with size and charge barriers of the glomerular filter were investigated using native (nf) and cationized (cf) ferritin as tracer probes. in proteinuric animals large numbers of nf molecules perfused from the abdominal aorta were observed to cross the glomerular basement membrane (gbm) and enter sd. the concentration of nf molecules was higher in gbm segment ... | 1991 | 1806651 |
| transport of peptides in renal brush border membrane vesicles. suitability of 125i-labelled tyrosyl peptides as substrates. | three tyrosine-containing peptides (tyr-pro, tyr-pro-phe and tyr-pro-phe-pro) were [125i]iodo-labelled and their uptake characteristics in renal brush border membrane vesicles was investigated to assess the suitability of these peptides as substrates in peptide transport studies. hydrolysis of these peptides during uptake measurements was avoided by using brush border membrane vesicles prepared from the kidneys of japan fisher 344 rats which genetically lack dipeptidylpeptidase iv activity. the ... | 1991 | 1681904 |
| development of lung metastases after curative intraperitoneal chemotherapy in a rat colon cancer model. | a model of colon peritoneal carcinomatosis was developed by injecting 5 x 10(7) viable tumor cells intraperitoneally into fisher 344 rats. all 40 control rats developed bulky abdominal tumor with ascites and died of peritoneal carcinomatosis and bowel obstruction (median survival 5 weeks). one day after tumor implantation, treatment group rats received a single intraperitoneal injection of single agent or combination chemotherapy. the most active intraperitoneal single agents were 5-fluorouracil ... | 1991 | 1990226 |
| distribution of chromium within cells of the blood. | although a number of investigators have examined the uptake of chromium in red blood cells (rbc) or whole blood, little is known about chromium uptake in white blood cells (wbc). radiolabeled chromium (51cr) was used to determine chromium uptake and distribution. isolated rbc and enriched wbc populations were exposed in vitro to potassium chromate (cr+6) and uptake was determined over a 2-hr time period. exposure of either rat or human blood cells to 50 microm k2cro4 for 2 hr resulted in greater ... | 1991 | 2006503 |
| amiodarone- and desethylamiodarone-induced pulmonary phospholipidosis, inhibition of phospholipases in vivo, and alteration of [14c]amiodarone uptake by perfused lung. | amiodarone (am) pulmonary phospholipidosis in patients receiving this drug is well recognized. we investigated the in vivo phospholipidosis-inducing potency of am and its major nonpolar metabolite, desethylamiodarone (dea), in rats, their ability to inhibit phospholipases, and also the effects on pulmonary uptake of [14c] am. fisher-344 male rats (200 to 250 g) were given am or dea (100 mg/kg/d orally) for 2, 7, or 21 d. food consumption and body weight gain were significantly reduced by both am ... | 1991 | 2015101 |
| effects of perinatal alcohol exposure and dietary calcium supplements on skeletal and dental growth in rats. | osteoporosis, hypocalcemia and skeletal size reduction are all common correlates of perinatal alcohol exposure. the present study assesses the effects of dietary calcium supplements on reversing perinatal alcohol-induced osteopenia. one hundred and twenty-nine offspring from fisher 344 rats received 14% v/v alcohol in tap water from conception to weaning or 3 months of age followed by dietary calcium supplements (230 mg/kg/day) to 6 months of age. significant group effects (p less than 0.001) we ... | 1991 | 2028725 |
| high-dose epinephrine improves the return of spontaneous circulation rates in human victims of cardiac arrest. | to evaluate the return of spontaneous circulation (rsc) rates in human victims of cardiac arrest treated with standard doses of epinephrine (sde) or high-dose epinephrine (hde). | 1991 | 2064091 |
| bacillus calmette-guérin immunization in infants born to hiv-1-seropositive mothers. | during the prospective follow-up of 64 babies at risk for perinatal hiv-1 infection because their mothers were seropositive, and of 130 control babies whose mothers were seronegative, we studied the occurrence of complications of bacillus calmette-guérin (bcg) immunization and its ability to induce cutaneous reactivity to tuberculin. babies born both to hiv-1-positive and hiv-1-negative mothers received bcg immunization during their first month of life according to the expanded programme on immu ... | 1991 | 2031692 |
| expression of p53 mutant protein(s) in diethylnitrosamine-induced foci of enzyme-altered hepatocytes in male fischer-344 rats. | several types of human and animal tumors have been shown to carry mutations in the p53 gene. while the translation product of the wild type gene has tumor suppressor properties, mutant alleles of the gene produce proteins that can cooperate with other oncogene products in transforming cells. in this paper, evidence is presented indicating that a p53 gene mutation(s) occurs in foci of enzyme-altered hepatocytes induced by diethylnitrosamine in male fisher-344 rats. the evidence was obtained by me ... | 1991 | 2044196 |
| increased frequency of infection after open reduction of fractures in patients who are seropositive for human immunodeficiency virus. | in a prospective study of 214 patients who had elective extensive operations for fractures, we compared the relative frequencies of postoperative infections in the seventeen patients who were seropositive for human immunodeficiency virus and had associated clinical symptoms, in the twenty-six patients who were seropositive and had no associated clinical symptoms, and in the 171 patients who were seronegative. the relative frequency of postoperative infection was significantly higher in patients ... | 1991 | 2045392 |
| working memory and the aged rat: deficient two-choice win-stay water-escape acquisition and retention. | twelve aged (22 months), 12 adult (12 months) and 12 young (3 months) male fisher 344 rats were compared on acquisition of a two-choice win-stay (spatial delayed matching-to-sample) water-escape working memory task. the 7 aged, 10 adult and 10 young animals that attained criterion were then compared on performance of the task when the retention interval (ri) was 5, 15, 30, 60 and 120 min. they were also compared on retention after being forced to perform an unsolvable water-escape task in a seco ... | 1991 | 2062901 |
| synthesis and in vivo photodynamic activity of some bacteriochlorin derivatives against bladder tumors in rodents. | bacteriochlorins have been suggested as potential photosensitizers for use in photodynamic therapy. we have shown that bacteriochlorin-like macrocycles can be generated through cyclization of either 5,10- or 5,15-bis[(ethoxycarbonyl)vinyl]porphyrins; however, the resulting products are rapidly decomposed on exposure to air. more stable systems can be generated by diels-alder reactions between dienophiles such as dimethyl acetylenedicarboxylate or tetracyanoethylene, and vinylporphyrinones. altho ... | 1991 | 2066985 |
| acute effects of interleukin-2 on lung mechanics and airway responsiveness in rats. | we studied the acute effects of interleukin-2 (il-2), the principal lymphokine responsible for lymphocyte proliferation, on lung mechanics and airway responsiveness to methacholine (mch) in rats. lewis (n = 12) and fisher 344 (n = 13) rats were anesthetized and intubated, and intravenous and intra-arterial lines were inserted. il-2 (750,000 u/kg) was infused intravenously over 2 to 4 min into seven lewis and seven fisher rats, and vehicle alone was administered to five lewis and six fisher rats. ... | 1991 | 1990957 |
| metabolic responses to swimming exercise in streptococcus pneumoniae infected rats. | the present study was performed to determine whether alterations in fuel reserves or energy substrate utilization might explain the performance decrements that occur in bacterial infections. male fisher-dunning rats were studied at 24, 48, and 72 h after inoculation with streptococcus pneumoniae. rats were either sedentary or subjected to a 2-h swimming session at these three time points (n = 10 in each group). a more than 60% reduction (p less than 0.01) in performance capacity was observed on ... | 1991 | 2056898 |
| effect of mild hyperthermia on the ischemic infarct volume after middle cerebral artery occlusion in the rat. | we investigated the effect of mild whole-body hyperthermia (40 degrees c) on a permanent middle cerebral artery occlusion (mcao) model in fisher rats by subjecting them to mcao under the following conditions: (1) normothermia (n = 20); (2) hyperthermia (n = 14) before (1 hour), during, and after (1 hour) mcao; and (3) post-mcao hyperthermia (n = 14) for 1 hour. we measured brain and body temperatures during the experiment using micro-thermocouples and blood-brain-barrier (bbb) permeability using ... | 1991 | 2067642 |
| photocytotoxicity of anthracyclines upon laser excitation in their long-wavelength absorption bands. | newly synthesized daunomycin derivatives with red-shifted absorption compared to the parent molecule are shown to be able to photosensitize cells in vitro upon excitation with either argon or argon-pumped dye laser. administering 86 j/cm2 total fluence (1 h irradiation) to fisher rat thyroid cells during 2 h incubation with either daunomycin (excitation wavelength: 488 nm) or 5-iminodaunomycin (595 nm) produced cell killing at doses (about 2.7 x 10(-7) m for 50% cell survival) which were not tox ... | 1991 | 2068268 |
| age-related effects of chronic ethanol intake on vitamin a status in fisher 344 rats. | the present study was designed to investigate the interaction of age and ethanol on vitamin a status in rats. rats aged 2 and 19 mo were fed a liquid diet containing 36% of total energy as ethanol or pair-fed a diet containing isoenergetic carbohydrate in place of ethanol. after 3 wk older rats had lower serum retinol (p = 0.04) and higher vitamin a concentrations in liver (p = 0.0001), esophagus (p = 0.0001) and the proximal (p = 0.03) and distal (p = 0.0001) colon than younger animals. hepatic ... | 1991 | 2007903 |
| effect of dietary menhaden, canola and partially hydrogenated soy oil supplemented with vitamin e upon plasma lipids and platelet aggregation. | male fisher rats were fed chow diets for two weeks after which they were divided into seven groups of ten rats each and fed 20% canola, 20% menhaden, 20% partially hydrogenated soy oil (phso) or chow only, with or without 500 mg/kg dietary vitamin e in chow containing 2% cholesterol for six weeks. triglycerides were lower in the menhaden group and were essentially the same in the e supplemented groups as in their unsupplemented cohorts. plasma cholesterol was higher in the canola, and lower in t ... | 1991 | 2028442 |
| differential dna-protein crosslinking in lymphocytes and liver following chronic drinking water exposure of rats to potassium chromate. | carcinogenic chromium (vi) compounds are persistent environmental contaminants with potential for human exposure through drinking water. one lesion associated with chromium (vi) exposure is the formation of dna-protein crosslinks (dpc). in an attempt to develop markers of chromium exposure, the formation of dpc in lymphocytes was investigated. fisher 344 rats were exposed to k2cro4 in their drinking water for 3 and 6 weeks at concentrations of 100 and 200 ppm chromium. no dpc could be detected i ... | 1991 | 2038750 |
| structural characterization of the lipid a component of pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides. | the structure of the lipid a component of lipopolysaccharides isolated from two wild-type strains (fisher 2 and 7) and one rough mutant (pac 605) of pseudomonas aeruginosa was investigated using chemical analysis, methylation analysis, combined gas-liquid chromatography/mass spectrometry, laser-desorption mass spectrometry and nmr spectroscopy. the lipid a backbone was found to consist of a pyranosidic beta 1,6-linked d-glucosamine disaccharide [beta-d-glcpn-(1----6)-d-glcpn], phosphorylated in ... | 1991 | 1904818 |
| central regulation of stress responses: regulation of the autonomic nervous system and visceral function by corticotrophin releasing factor-41. | our understanding of the role of crf in mediating integrated endocrine, autonomic and visceral stress responses is rudimentary at best. delineating the large number of neurochemical factors that influence the activity of crf-containing hypophyseotrophic neurones offers one direction for future research in this area. another approach might be to examine the neuropharmacological actions of transmitters which are co-localized within crf-containing neurones. for example, crf and dynorphin-related pe ... | 1991 | 2039427 |
| experimental intravesical therapy for superficial transitional cell carcinoma in a rat bladder tumor model. | a rodent bladder cancer model that is induced by intravesical instillation of n-methyl-n-nitrosourea (mnu) was characterized. cohorts of four to five week old female fisher 344 rats received four biweekly 1.5 mg. doses of intravesical mnu and were sacrificed at various intervals. by week 13 all animals had flat atypia and/or papillary transitional cell tumors, and 67% of the lesions were moderately (grade ii) or poorly differentiated (grade iii). by week 20, 83% had gross muscle invasive tumors ... | 1991 | 1997724 |
| when genius errs: r.a. fisher and the lung cancer controversy. | r.a. fisher's work on lung cancer and smoking is critically reviewed. the controversy is placed in the context of his career and personality. although fisher made invaluable contributions to the field of statistics, his analysis of the causal association between lung cancer and smoking was flawed by an unwillingness to examine the entire body of data available and prematurely drawn conclusions. his views may also have been influenced by personal and professional conflicts, by his work as a consu ... | 1991 | 2000852 |
| rorschach assessment of psychological functioning in sexually abused girls. | we measured psychological functioning in a group of 79 black females between the ages of 5 and 16 and a comparison group of nonabused girls using the rorschach. in addition to exner's (1985) comprehensive system, the elizur (1949) rorschach content test scale (rct), the mutuality of autonomy scale (moa; urist, 1977; urist & shill, 1982), and the barrier and penetration scales (fisher & cleveland, 1968) were used. sexually abused girls were found to show more disturbed thinking, to experience a h ... | 1991 | 2002437 |
| identification of an epitope encoded in the env gene of friend murine leukemia virus recognized by anti-friend virus cytotoxic t lymphocytes. | we have previously shown that strong epitopes recognized by anti-friend virus (fv) cytotoxic t lymphocytes (ctl) in h-2b mice are encoded in both the env and gag/pol regions of the helper friend leukemia virus genome. two approaches have been used to identify these epitopes. at the nucleic acid level, we have constructed env genes with either of two in-frame deletions: pkr2, an env gene with a 681-bp deletion in the gp70 region and inserted into the psv2-gpt-1 expression vector; and pkr1, an env ... | 1991 | 1704662 |
| amino acid sequence and post-translational modification of stem cell factor isolated from buffalo rat liver cell-conditioned medium. | stem cell factor (scf) isolated from culture medium conditioned by buffalo rat liver cells was subjected to detailed structural analysis. attempts at direct n-terminal sequencing of the factor indicated that its n terminus is blocked as pyroglutamic acid (zsebo, k. m., wypych, j., mcniece, i. k., lu, h. s., smith, k. a., karkare, s. b., sachdev, r. k., yuschenkoff, v. n., birkett, n. c., williams, l. r., satyagal, v. n., bosselman, r. a., mendiaz, e. a., and langley, k. e. (1990) cell 63, 195-20 ... | 1991 | 1708771 |
| t cell reactivity to an epitope of the mycobacterial 65-kda heat-shock protein (hsp 65) corresponds with arthritis susceptibility in rats and is regulated by hsp 65-specific cellular responses. | adjuvant arthritis (aa) can be induced in genetically susceptible rats by immunization with heat-killed mycobacteria suspended in mineral oil. from our analysis of arthritogenic t cell clone a2b, obtained from an arthritic lewis rat and specific for the 180-188 epitope of mycobacterial 65-kda heat-shock protein (hsp 65), the possible origin of aa was explained by the existence of a molecular mimicry of the 180-188 epitope with a cartilage-associated self antigen. we now have shown that lewis rat ... | 1991 | 1709871 |
| antibody-targeted photolysis. bacteriocidal effects of sn (iv) chlorin e6-dextran-monoclonal antibody conjugates. | antibody-targeted photolysis is a technique for damaging or killing cells using light and an antibody-bound photosensitizer. in the present study, immunoconjugates were constructed to selectively kill pseudomonas aeruginosa bacteria using tin (iv) chlorin e6 which was linked to dextran and then bound to the carbohydrate moiety of a monoclonal antibody specific for pseudomonas aeruginosa fisher type i polysaccharide antigen. killing of pseudomonas during mid-log phase growth was shown to be depen ... | 1991 | 1706578 |
| identification of t cell epitopes within a 23-kd antigen (p24) of toxoplasma gondii. | among the potentially vaccinating antigens, the products excreted/secreted by the parasite t. gondii have been demonstrated to be excellent candidates. the molecular cloning of one of these antigens (p24) present in excreted/secreted antigens (esa) has recently been carried out in our laboratory. the recombinant antigen p24 corresponds to a native molecule of 23 kd. we were interested in determining the main epitopes of the p24 antigen eliciting a t lymphocyte response using synthetic peptides d ... | 1991 | 1710549 |
| food restriction retards aging of the pineal gland. | the effects of chronic (40%) food restriction from 6 weeks of age were studied in aging male fisher 344 rats. when compared with 3-month-old, ad libitum fed rats, pineal n-acetyltransferase (nat) activity had declined to less than 30% and pineal and serum levels of melatonin to 40% after 28 months when feeding had been ad libitum. food restriction significantly retarded this development (p less than 0.05) giving nat and melatonin levels which were twice as high as in the ad libitum fed group. ni ... | 1991 | 1713529 |
| glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure. | we have recently described the identification, isolation, and characterization of a factor, termed stem cell factor (scf), which acts on primitive hematopoietic progenitors of the marrow. a soluble form of the factor was isolated from the conditioned medium of a rat cell line (zsebo, k. m., wypych, j., mcniece, i. k., lu, h. s., smith, k. a., karkare, s. b., sachdev, r. k., yuschenkoff, v. n., birkett, n. c., williams, l. r., satyagal, v. n., tung, w., bosselman, r. a., mendiaz, e. a., and langl ... | 1991 | 1717457 |
| photodynamic therapy using pheophorbide a and nd:yag laser. | the authors describe a new photodynamic therapy (pdt) method for malignant brain tumors. pheophorbide a (ph-a), the photosensitizer, has low toxicity, causes no skin sensitization and is activated with an acoustic q switched neodymium yttrium-argon-garnet (nd:yag) laser which achieves deep tissue penetration. the ph-a distribution in fisher 344 (f344) rats bearing rat t9 glioma at 24 hours after intravenous injection was very low in the normal brain tissue, but significantly higher in the t9 gli ... | 1991 | 1717860 |
| angiopolarity: a new design parameter for cell transplantation devices and its application to degradable systems. | the purpose of this study was to obtain directional angiogenesis of small blood vessels and capillaries to an implant made from a resorbable polymer for hepatocyte transplantation. it was intended to mimic the native acinar structure of the liver in form to facilitate replication of the cells and organ growth. the implant device structure was designed for injection to minimize surgical trauma. hollow microspheres with an open porous wall structure and one large central opening were made from pol ... | 1991 | 1721514 |
| purification and n-terminal amino acid sequence of a chondroitin sulphate/dermatan sulphate proteoglycan isolated from intima/media preparations of human aorta. | a proteoglycan (pg) was purified to homogeneity from intima/media preparations of human aorta specimens by the following chromatographic steps: sepharose q anion exchange, sepharose cl-4b size exclusion, hydroxyapatite, monoq anion exchange and tsk g 4000 sw size exclusion. the purity of the preparation was established by sds/page using direct staining by silver or dimethylmethylene blue, as well as by western blots of biotin-labelled samples. the electrophoretic mobility of the native pg was le ... | 1991 | 1848758 |
| purification and properties of the h(+)-nicotinamide nucleotide transhydrogenase from rhodobacter capsulatus. | 1. h(+)-transhydrogenase from rhodobacter capsulatus is an integral membrane protein which, unlike the enzyme from rhodospirillum rubrum, does not require the presence of a water-soluble component for activity. 2. the enzyme from rb. capsulatus was solubilised in triton x-100 and subjected to ion-exchange, hydroxyapatite and then gel-exclusion column chromatography. sds/page of the purified enzyme revealed the presence of two polypeptides with apparent mr 53,000 and 48,000. other minor component ... | 1991 | 1849819 |
| biochemical quantitation and histochemical localization of glucose-6-phosphate dehydrogenase activity in the olfactory system of adult and aged rats. | the activity of glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the hexose monophosphate shunt, was examined in olfactory epithelium, respiratory epithelium, olfactory bulb, and occipital cortex in fisher 344 rats aged 4 and 24 months. marked differences in this enzyme were found in olfactory compared to nonolfactory tissues. olfactory epithelium and olfactory bulb have much greater glucose-6-phosphate dehydrogenase activity than respiratory epithelium and occipital cortex at both ... | 1991 | 1922658 |
| cholestasis without cirrhosis alters regulatory liver gene expression and inhibits hepatic regeneration. | partial hepatectomy (ph) initiates cellular signals for regeneration. sequential expression of nuclear and cytosolic protooncogenes accompanies the restoration of normal liver function and architecture. although cirrhosis is known to inhibit liver regeneration, the effects of noncirrhotic cholestasis on hepatocellular proliferation, differentiation, and regulatory gene expression are unknown. to examine this, 25 male fisher rats underwent common bile duct ligation and division. a 47% +/- 5% ph w ... | 1991 | 1858028 |
| [does tissue ph measurement quantify circulation in the transplanted bronchus in lung transplantation]. | ischemia of the airway is the main cause of severe complications in lung transplantation. tissue-ph is used as an indicator of ischemia in the myocardium and various other tissues. we developed a ph needle probe which can be applied through a bronchoscope to enable repeated measurements of the ph in the bronchus mucosa. in 8 conditioned mongrel dogs (20-25 kg) an autotransplant of a 4-ring segment of the thoracic trachea was performed and wrapped with an omental pedicle flap in 4 of them (omentu ... | 1991 | 1864741 |
| the effect of hypertonic saline resuscitation on bacterial translocation after hemorrhagic shock in rats. | translocation of enteric bacteria occurs in rats after hemorrhagic shock. a proposed mechanism involves intestinal mucosal injury by hypoperfusion. recent work suggests that moderate hypovolemia causes gut arteriolar constriction, which is ameliorated by hypertonic saline resuscitation. bacterial translocation should, therefore, be reduced when hypertonic saline (hs) is used as the resuscitative fluid. seventy-eight sprague-dawley rats were anesthetized and subjected to 30 minutes of hemorrhagic ... | 1991 | 1925957 |
| [development of peritubular myoid cells in the rat testis]. | in the testes of 47 embryos, fetuses and newborn rats (fisher strain), until the 15th day of life, the appearance and numerical dynamics of "premyoid" and myoid cells were analysed by light microscopy. the material was prepared routinely (fixed in bouin fluid, embedded in paraffin, and cut to the thickness of 7 micrometers). the embryos of the 12th to 17th day of gestation were serially sectioned in toto, while fetal testes on the 18th to 21th day of gestation were removed and serially cut as we ... | 1991 | 1875715 |
| enhancement of antitumor drug cytotoxicity via laser photoactivation. | we investigate the efficacy of daunomycin, some imino- and amino-substituted daunomycin analogues and the disubstituted aminoanthracenedione, mitoxantrone, in photosensitizing short-term cell kill upon irradiation in the long wavelength visible range, during incubation of fisher rat thyroid cells with the drugs. while all compounds exhibit similar cytocidal effects on our cell line, in the absence of irradiation, administering 86 j/cm2 at wavelengths either coincident or close to drug absorption ... | 1991 | 1886938 |
| treatment of corneal allograft rejection with the cytotoxin il-2-pe40. | il-2-pe40 is a recombinant chimeric protein composed of il-2, fused to a modified pseudomonas exotoxin. this molecule is extremely toxic to activated t cells expressing high-affinity il-2r. we used this new molecule for selective immunosuppression to treat corneal allograft rejection in the rat, using fisher and lewis rats, a strain combination differing only in medial and minor histocompatibility antigens. the effect of il-2-pe40 on the immunologic response was studied using both a heterotopic ... | 1991 | 1897019 |
| the role of cytophilic igg3 antibody in t cell-mediated resistance to infection with the extracellular bacterium, pseudomonas aeruginosa. | previous studies have demonstrated that t lymphocytes from mice immunized with a high m.w. polysaccharide ag from fisher-devlin immunotype i pseudomonas aeruginosa can adoptively transfer protection against challenge with the homologous bacterial strain to susceptible mice. this t cell-mediated resistance has been found to be b cell dependent, although serum from immunized mice is incapable of passively transferring protection to nonimmune mice. the current studies demonstrate that t cells from ... | 1991 | 1898604 |
| norepinephrine and isoproterenol increase the phosphorylation of synapsin i and synapsin ii in dentate slices of young but not aged fisher 344 rats. | a number of recent reports have suggested that norepinephrine (ne) produces a form of synaptic enhancement that resembles long-term potentiation (ltp). ltp, thought to be an electrophysiological correlate of memory, in part involves an augmentation of transmitter release. although the effects of ne have not been unequivocally linked to ltp, it is clear that ne can produce increased transmitter release in the dentate gyrus of the hippocampus. the purpose of this study was to determine whether ne ... | 1991 | 1900942 |
| single-dose tumor necrosis factor protection against endotoxin-induced shock and tissue injury in rats. | tumor necrosis factor (tnf), a macrophage product released in response to endotoxin and other stimuli, has been shown to be a central mediator of endotoxin or septic shock. however, its highly conserved and wide-ranging physiological effects suggest that it may also be an essential cytokine in the host defense against acute bacterial infection or sepsis. a single nontoxic dose of human recombinant tnf administered intravenously 24 h prior to a lethal infusion of escherichia coli lipopolysacchari ... | 1991 | 1937748 |
| growth characteristics and metastatic potential of seven intestinal carcinoma lines serially passaged in syngeneic rats. | transplantable tumour lines were obtained from one duodenal carcinoma induced by n-methyl-n'-nitro-n-nitrosoguanidine in the lewis rat and from six colonic carcinomas induced by 1,2 dimethylhydrazine in bdix or fisher rats. the tumours were serially transplanted by the subcutaneous route into homologous syngeneic rats. the seven tumours differ from one another in their histological structure, five of them being well or moderately differentiated adenocarcinomas, and in their capacity to produce n ... | 1991 | 1900965 |
| characterization of the mutation responsible for aspartylglucosaminuria in three finnish patients. amino acid substitution cys163----ser abolishes the activity of lysosomal glycosylasparaginase and its conversion into subunits. | the mutation that causes a deficiency of the lysosomal amidase, glycosylasparaginase, has been characterized in fibroblasts from three finnish patients diagnosed with aspartylglucosaminuria (agu). the polymerase chain reaction was used to amplify the glycosylasparaginase protein coding sequence from the three agu patients in order to compare them to the normal sequence from a full-length human placenta cdna clone hpasn.6 (fisher, k.j., tollersrud, o.k., and aronson, n.n., jr. (1990) febs lett. 2 ... | 1991 | 1904874 |
| functional enhancement of intrastriatal dopamine-containing grafts by the co-transplantation of sciatic nerve tissue in 6-hydroxydopamine-lesioned rats. | peripheral nerve "bridges" demonstrate the ability to facilitate axonal growth and regenerate adult and fetal central nervous system tissue. the purpose of this study was to determine if co-grafted peripheral nerve tissue could enhance the ability of fetal dopamine (da) cell transplants to reinnervate host striatum that had been denervated unilaterally. male fisher-344 rats were unilaterally lesioned with 6-hydroxydopamine to eliminate the nigrostriatal da pathway. a total of 31 rats demonstrate ... | 1991 | 1907926 |
| isonymy in records of births and deaths in ferrara. | surname distributions were studied in records of male and female births in ferrara in the period 1982-89, and in records of male and female deaths in the same period. average year of birth and standard deviation was 1985 +/- 2.3 for the birth series, and 1912 +/- 14.4 for the death series. then the surname distributions, in two independent samples at an average distance of 73 years, were compared. it was observed that random isonymy within series, which depends on the shape of the distribution, ... | 1991 | 1952797 |