| serotype-specific inhibition of colonization of the tonsils and nasopharynx of calves after pasteurella haemolytica serotype a1 after vaccination with the organism. | vaccination of cattle with a tissue culture-derived pasteurella haemolytica serotype 1 vaccine elicited a serotype-specific inhibition of nasal and tonsillar colonization by the homologous serotype under field conditions. calves (n = 101) originated from a single farm, where half the calves were vaccinated. the calves were delivered to an order-buyer barn 105 days later, and given a second dose of vaccine. at the order-buyer barn, calves were mixed with 27 calves, some of which had clinical sign ... | 1994 | 7978650 |
| an outbreak of infectious laryngotracheitis in california broilers. | infectious laryngotracheitis (ilt) was diagnosed as the cause of an outbreak of respiratory disease in broiler chickens in california. the classical form of ilt is characterized by dyspnea, gasping, coughing, and expectoration of bloody exudate. most of the broilers submitted to the diagnostic laboratory showed a non-classical presentation of ilt, in which mucoid tracheitis and conjunctivitis were the most consistent lesions. historically, most of the ilt cases diagnosed in our laboratory have c ... | 1994 | 8002892 |
| induction of acute bronchopneumonia in mice by intrabronchial inoculation of pasteurella haemolytica serotype 1. | dose dependent pulmonary lesions of acute bronchopneumonia were induced in male, outbred swiss webster mice by intrabronchial inoculation of pasteurella haemolytica. five exponential dilutions ranging from 5 x 10(4) to 5 x 10(8) colony forming units per ml (cfu/ml) of pasteurella haemolytica serotype 1 were inoculated into five groups of mice. mice were killed by cervical dislocation 24 hours postinoculation. pulmonary lesions occurred in mice of all five groups, however, 5 x 10(7) cfu/ml was th ... | 1994 | 8004544 |
| sialoglycoprotease of pasteurella haemolytica a1: detection of antisialoglycoprotease antibodies in sera of calves. | log phase culture supernate from pasteurella haemolytica biotype a, serotype 1 contains a proteolytic enzyme specific for o-sialoglycoproteins. using two methods, western immunoblotting and enzyme neutralization assay, it was demonstrated that certain bovine sera from two previous p. haemolytica a1 vaccination and challenge trials contained antibodies (ab) (isotypes igg1 and igg2 on western immunoblot) to the sialoglycoprotease (gcp). in these trials, selected calves were vaccinated twice with e ... | 1994 | 8004547 |
| variation in outer-membrane protein and lipopolysaccharide profiles of pasteurella haemolytica isolates of serotypes a1 and a2 obtained from pneumonic and healthy cattle. | the outer-membrane protein (omp) and lipopolysaccharide (lps) profiles of 29 isolates of pasteurella haemolytica serotypes a1 (18 isolates) and a2 (11 isolates), obtained from pneumonic (13 isolates) or healthy (16 isolates) cattle, were compared by sds-page and western blot analysis. coomassie-blue-stained omp profiles of serotype a1 and a2 isolates could be distinguished from each other by differences in both major and minor proteins. whereas the omp profiles of the serotype a1 isolates were e ... | 1994 | 8012599 |
| administration of a bolus formulation of baquiloprim and sulphadimidine to calves: plasma concentration--time profiles and efficacy in suppressing experimental pneumonic pasteurellosis. | after administration of a bolus containing 0.8 g baquiloprim and 7.2 g sulphadimidine to six calves, plasma concentrations of these compounds exceeded mics for pasteurella haemolytica from about 3 hours to at least 48 hours. experimental infection of three calves with 2.8 x 10(10) p. haemolytica type a1 organisms by the endobronchial route resulted in extensive pneumonic lesions, and smaller, similar lesions were found post mortem in two other calves which had received the same inoculum. nine si ... | 1994 | 8017029 |
| evaluation of an orally administered vaccine, using hydrogels containing bacterial exotoxins of pasteurella haemolytica, in cattle. | poly(methacrylic acid) hydrogels were tested for oral delivery of a vaccine against pasteurella haemolytica infection in cattle. culture supernatants of p haemolytica, the most common bacterium associated with pneumonia in cattle, were used as the antigens in the vaccine. hydrogels containing culture supernatants were administered orally to calves. calves were then challenge-exposed with virulent p haemolytica. calves were euthanatized 3 days after challenge exposure. the lungs of each calf were ... | 1994 | 8017696 |
| fatal pneumonia following inoculation of healthy bighorn sheep with pasteurella haemolytica from healthy domestic sheep. | in a series of three experiments, isolates of pasteurella haemolytica biotype a, serotype 2, ribotype reference wsu-1, from healthy domestic sheep, were inoculated intratracheally into eight bighorn sheep (ovis canadensis canadensis) and seven domestic sheep with doses of bacteria ranging from 5.3 x 10(8) to 8.6 x 10(11) colony forming units. seven of eight inoculated bighorn sheep died from acute pneumonia within 48 hr of inoculation, whereas all seven domestic sheep inoculated with comparable ... | 1994 | 8028096 |
| a reliable transport method for isolating pasteurella haemolytica from bighorn sheep. | we compared three transport methods for the recovery of pasteurella haemolytica from pharyngeal swabs collected under field conditions from 42 bighorn sheep (ovis canadensis) in one captive and three free-ranging populations. transport methods included: amies medium with charcoal, transported on ice, and cultured on blood agar within 24 hr; direct plating on blood agar, transported on heating pads, and incubated at 37 c within 8 hr of collection; and phosphate buffered glycerol (pbg), transporte ... | 1994 | 8028114 |
| characterization of a restriction endonuclease, phai, from pasteurella haemolytica serotype a1 and protection of heterologous dna by a cloned phai methyltransferase gene. | pasteurella haemolytica is the leading cause of economic loss to the beef cattle industry in the united states and an important etiologic agent worldwide. study of p. haemolytica is hindered by researchers' inability to genetically manipulate the organism. a new restriction endonuclease, phai, an isoschizomer of sfani (r. j. roberts, methods enzymol. 65:19-36, 1980), was isolated from p. haemolytica serotype 1, strain nadc-d60, obtained from pneumonic bovine lung. phai recognizes the 5-base nonp ... | 1994 | 8031094 |
| molecular gene cloning and nucleotide sequencing and construction of an aroa mutant of pasteurella haemolytica serotype a1. | the aroa gene of pasteurella haemolytica serotype a1 was cloned by complementation of the aroa mutation in escherichia coli k-12 strain ab2829. the nucleotide sequence of a 2.2-kb fragment encoding aroa predicted an open reading frame product 434 amino acids long that shows homology to other bacterial aroa proteins. several strategies to inactivate aroa were unsuccessful. gene replacement was finally achieved by constructing a replacement plasmid with aroa inactivated by insertion of a p. haemol ... | 1994 | 8031095 |
| construction of conjugative shuttle and suicide vectors for pasteurella haemolytica and p. multocida. | a shuttle cloning vector, paka16, and suicide derivatives paka19 and paka22 have been developed for gene transfer to pasteurella haemolytica and p. multocida. paka16 was constructed by insertion of the lacz alpha-peptide-encoding region and a multiple cloning site into a plasmid which was originally isolated from p. haemolytica serotype a1. the vector encodes ampicillin resistance, and contains at least 14 unique restriction sites and the property of phenotypic identification of recombinant clon ... | 1994 | 8045428 |
| distribution of anti-cd68 (ebm11) immunoreactivity in formalin-fixed, paraffin-embedded bovine tissues. | a commercially acquired anti-human macrophage antibody (anti-cd68; ebm11) was used in an immunocytochemical technique to detect macrophages in formalin-fixed, paraffin-embedded tissues from cattle, pigs, humans, rats, turkeys, dogs, and cats. in healthy cattle, the antibody labeled alveolar macrophages, pulmonary intravascular cells (presumably intravascular macrophages), and macrophage-like cells in other tissues. in bovine lungs infected with pasteurella haemolytica, ebm11 antibody labeled 95% ... | 1994 | 8053129 |
| monofrequency forced oscillation technique for the investigation of pulmonary function in calves: in vitro and in vivo studies. | parameters derived from the non-invasive and simple monofrequency forced oscillation technique were compared with classical parameters of ventilatory mechanics in order to assess its usefulness for the investigation of pulmonary function in calves. to facilitate this comparison, theoretical derivations were coupled with in vitro measurements, using an artificial lung model, and with in vivo studies. these studies compared the oscillatory resistance parameters (ros and re) and the respiratory sys ... | 1994 | 8073189 |
| transferrin receptors on ruminant pathogens vary in their interaction with the c-lobe and n-lobe of ruminant transferrins. | the interaction between ruminant transferrins and receptor proteins on the surface of the ruminant pathogens pasteurella haemolytica, haemophilus somnus, pasteurella multocida, haemophilus agnii, and moraxella bovis was evaluated by a combination of binding assays and affinity isolation procedures. membranes isolated from p. haemolytica, p. multocida, and h. agnii were capable of binding sheep, goat, and cattle transferrins whereas binding by membranes from h. somnus and m. bovis was specific fo ... | 1994 | 8076248 |
| the effect of a combined pasteurella haemolytica and haemophilus somnus vaccine and a modified-live bovine respiratory syncytial virus vaccine against enzootic pneumonia in young beef calves. | | 1994 | 8076280 |
| a leukotoxin nonproducing mutant of pasteurella haemolytica. phagocytosis and killing by bovine polymorphonucleocytes. | | 1994 | 8080187 |
| antimicrobial susceptibility of pasteurella haemolytica and pasteurella multocida isolated from pneumonic ovine lungs. | the in vitro susceptibilities of pasteurella haemolytica biotypes a and t and p multocida from pneumonic ovine lungs to penicillin, ampicillin, oxytetracycline, chloramphenicol, erythromycin, kanamycin, neomycin, gentamicin, streptomycin and lincomycin were determined by the disk diffusion method. all the isolates were sensitive to chloramphenicol and resistant to lincomycin. the isolates of p haemolytica biotype a were consistently more sensitive to penicillin, ampicillin, oxytetracycline, eryt ... | 1994 | 8085324 |
| report of the thirteenth meeting of the oie ad hoc group on non tsetse-transmitted animal trypanosomoses. | there is increasing interest in many parts of the world in trypanosomoses other than those transmitted by tsetse flies, as shown by numerous research projects and field studies. the refinement of techniques for studying the behaviour of trypanosomes (techniques of molecular biology) in axenic culture or in the parasitised host has led to progress in diagnosis and immunology, and a rational approach to chemotherapy and chemoprophylaxis of these infections. field trials of enzyme-linked immunosorb ... | 1993 | 8100154 |
| morbidity and mortality in a large irish feedlot; microbiological and serological findings in cattle with acute respiratory disease. | a survey of morbidity and mortality was undertaken in a slatted unit containing 6399 beef cattle over a 6 month period. the mortality rate was 0.78% and the morbidity rate was 12.7%. the interval from arrival to morbidity was 25.5 days +/- 0.9. significantly more sick cattle were identified on either mondays or tuesdays than were seen on saturdays or sundays (p < 0.005). the mean interval to mortality (all diseases) was 48.5 days +/- 7.4. respiratory disease was the most frequently recorded dise ... | 1993 | 8111615 |
| pasteurella haemolytica serotypes isolated in northern ireland during 1989-1991. | pasteurella haemolytica isolates from clinical veterinary samples were serotyped over a three year period to determine the distribution of strains in northern ireland, to compare the results with the recorded distribution in the remainder of great britain and to extend information on the geographical prevalence of the serotypes. four hundred and nine typable and 91 untypable strains were isolated from 490 animal cases. from 127 typable cattle isolates, the commonest serotype was a1, most frequen ... | 1993 | 8111616 |
| first reported isolation of mycoplasma bovis from pneumonic calves in northern ireland. | | 1993 | 8116160 |
| immunogens of pasteurella. | the family pasteurellaceae pohl contains gram-negative, facultatively anaerobic and fermentative bacteria of the genera pasteurella, haemophilus, and actinobacillus. approximately 20 different species of the genus pasteurella have been identified using phenotypic and genetic analyses. of these species, p. multocida and p. haemolytica are the most prominent pathogens in domestic animals causing severe diseases and major economic losses in the cattle, swine, sheep, and poultry industries. mechanis ... | 1993 | 8116191 |
| treatment of calf pneumonia with tilmicosin. | the therapeutic effect of a single injection of tilmicosin (10 mg/kg body mass) against natural cases of pneumonia of calves, compared to other conventionally used antibiotics, was examined under field conditions. the trials were conducted in three cattle herds and included the treatment of 135 calves with 82 controls. the animals' age ranged from 1 week to 4 months. prior to the treatment and in case of death bacteriological examinations from nasal swabs and from the lungs were carried out. cli ... | 1993 | 8116500 |
| comparison between the minimal inhibitory concentration of tilmicosin and oxytetracycline for bovine pneumonic pasteurella haemolytica isolates. | the minimal inhibitory concentration (mic) of tilmicosin and oxytetracycline was determined for pasteurella haemolytica isolated from dutch cattle in 1991. the agar dilution method was used. of the pasteurella haemolytica strains examined, 96% (24) had a mic for tilmicosin of 2 micrograms/ml or lower; in one strain the mic was 8 micrograms/ml. the mic for oxytetracycline was equal to or higher than 64 micrograms/ml in 19 strains (76%), whereas for 5 strains the mic was 1 microgram/ml and for 1 s ... | 1993 | 8122359 |
| characterization of escherichia coli isolated from the tonsils of cattle. | during our studies on tonsillar colonization by pasteurella haemolytica, we consistently found escherichia coli to be one of the most prominent and prevalent bacterial species in the tonsils of healthy cattle. since tonsillar isolates have not been characterized, we grouped 124 isolates from 87 healthy cattle from eight sources by hemolytic zone size and by carbon source utilization and probed them for selected virulence genes. they formed 3 groups by hemolytic zone size and 18 groups (of 2 to 3 ... | 1994 | 8126195 |
| disturbances in ex vivo vascular smooth muscle responses following exposure to pasteurella haemolytica vaccines. | rats were vaccinated with saline (control) or one of the two commercially available pasteurella haemolytica vaccines presponse or precon-ph. animals were killed 3 days later and thoracic aorta removed for evaluation of the ex vivo biophysical responses to carbachol (cch). in some experiments, vascular endothelium was mechanically removed. vaccination of rats impairs the endothelial-dependent relaxation to cch. in vessels with endothelium removed, the contractile response to cch is converted into ... | 1993 | 8126761 |
| [detection of atypical toxin-producing pasteurella strains on the nasal mucosa of calves and their differential diagnostic limits for pasteurella haemolytica and pasteurella multocida]. | the bacterial colonisation on nasal mucous membranes of calves at age of 3, 5 to 5 months was investigated by cotton swabs. three pasteurella species were found (p. multocida subspecies multocida, p. haemolytica, p. avium) as monocausal infection as well as pluricausal infection. p. avium was characterised by colonial morphology, bacterioscopy, biochemical properties, polypeptide-pattern in sds-page and by electronmicroscopic investigation and was differentiated from p. multocida and p. haemolyt ... | 1994 | 8129703 |
| tissue factor expression in bovine endothelial cells induced by pasteurella haemolytica lipopolysaccharide and interleukin-1. | pasteurella haemolytica in cattle produces fibrino-hemorrhagic pleuropneumonia characterized by extensive pulmonary microvascular thrombosis and parenchymal necrosis. the purpose of this in vitro study was to determine if p. haemolytica lipopolysaccharide (lps) promotes vascular thrombosis by inducing a procoagulant state in vascular endothelial cells. after treatment of confluent monolayers of bovine pulmonary artery endothelial cells with various concentrations of either p. haemolytica lps or ... | 1994 | 8140726 |
| passive immunity to pasteurella haemolytica a1 in dairy calves: effects of preparturient vaccination of the dams. | dairy cows from five herds were assigned to receive a commercial pasteurella haemolytica vaccine or no vaccine at all, administered at six and three weeks before parturition. vaccination was associated with increased leukotoxin neutralizing serum antibody titers in the dams (p < 0.001), and with increased titers in colostrum (p < 0.001). vaccination of dams also had a significant association with increased passive leukotoxin neutralizing antibody titers in their calves (p < 0.001). vaccination w ... | 1994 | 8143250 |
| preparation of recombinant glycoprotease of pasteurella haemolytica a1 utilizing the escherichia coli alpha-hemolysin secretion system. | three murine monoclonal antibodies were prepared against the recombinant glycoprotease of pasteurella haemolytica a1 expressed in escherichia coli. these monoclonal antibodies were able to recognize the authentic glycoprotease from p. haemolytica a1 culture supernatant. a recombinant plasmid which contained most of the glycoprotease gene of p. haemolytica a1 fused with the secretion signal sequence from hlya of the e. coli alpha-hemolysin determinant was constructed. this recombinant plasmid exp ... | 1994 | 8150268 |
| bacteria isolated from nasal and tonsillar samples of clinically healthy rocky mountain bighorn and domestic sheep. | nasal and tonsillar samples were collected from 14 free-ranging clinically healthy rocky mountain bighorn sheep (ovis canadensis canadensis) and 10 domestic sheep (ovis aries). we identified 194 bacterial isolates, including 101 from bighorn and 93 from domestic sheep. of these isolates, 115 were gram-positive and 79 were gram-negative. staphylococcus species were the most numerous gram-positive organisms and had a higher incidence in samples from domestic than from bighorn sheep. in contrast st ... | 1994 | 8151809 |
| effects of modified cary and blair medium on recovery of nonhemolytic pasteurella haemolytica from rocky mountain bighorn sheep (ovis canadensis canadensis) pharyngeal swabs. | modified cary and blair transport medium (mcb) was evaluated for recovery of pasteurella spp. from pharyngeal swabs of healthy rocky mountain bighorn sheep (ovis canadensis canadensis). in experiment one, three pharyngeal swabs were collected from each of 25 bighorns. pasteurella haemolytica was recovered from 21 of 25 swabs tested almost immediately and from 16 of 25 swabs held in mcb medium at about 22 c for 24 hr before testing (p > 0.10). recovery of p. haemolytica decreased (p < 0.005) to 1 ... | 1994 | 8151818 |
| electron microscopy of phages in serotypes of actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans, actinobacillus ureae, haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, haemophilus parainfluenzae, pasteurella haemolytica and pasteurella multocida strains were examined by transmission electron microscopy for the presence of bacteriophages. phages were detected in serotype a (suny 75) and e (uoh 1705) and in the fresh clinical isolates uoh q1243 and uoh q1247 of a. actinomycetemcomitans. phages were not found in serotype b, c an ... | 1993 | 8152840 |
| regulation of major histocompatibility complex class ii expression by pasteurella haemolytica leukotoxin. | many properties have been associated with pasteurella haemolytica leukotoxin and other repeat-in-toxin toxins, including their cytotoxic activity on various cells of the lymphoid and nonlymphoid systems as well as their ability to modulate the immunological activity of lymphocytes and monocytes. in this study, we assessed the ability of p. haemolytica leukotoxin to affect the expression major histocompatibility complex (mhc) class ii molecules on bovine peripheral monocytes. peripheral blood mon ... | 1994 | 8168921 |
| cleavage of epitectin, a mucin-type sialoglycoprotein, from the surface of human laryngeal carcinoma cells by a glycoprotease from pasteurella haemolytica. | in this study we have assessed the action of a novel glycoprotease, secreted by the bovine pneumonia pathogen pasteurella haemolytica, on epitectin expressed on the surface of human laryngeal carcinoma (h.ep.2) cells. epitectin has been previously characterized as a high buoyant density glycoprotein of mass of over 350 kda extensively glycosylated on serine and threonine by small oligosaccharides. purified metabolically labeled epitectin was very effectively hydrolyzed by the glycoprotease. howe ... | 1994 | 8179312 |
| static dna bending and protein interactions within the pasteurella haemolytica leukotoxin promoter region: development of an activation model for leukotoxin transcriptional control. | in this study, we define cis-acting elements involved in regulation of the pasteurella haemolytica leukotoxin promoter. in place of a canonical promoter -35 sequence, the leukotoxin promoter contains four adenine-rich repeats of sequence ca6(c/t)a, phased at approximately 10-base intervals. dna fragments containing these repeats exhibit retarded mobility in polyacrylamide gels and permitted identification of a static dna bend in the promoter -70 region. deletion of the static dna bend caused a t ... | 1994 | 8179822 |
| effect of pasteurella haemolytica infection on alpha 1-acid glycoprotein and albumin concentrations in serum and subcutaneous tissue chamber fluid of calves. | thermoplastic tissue chambers were implanted subcutaneously in the paralumbar fossae of 10 calves. concentrations of alpha 1-acid glycoprotein (aag) and albumin in serum and subcutaneous tissue chamber fluid were measured before and after inoculation of pasteurella haemolytica into tissue chambers. two months after implantation, serum and tissue chamber fluid samples were collected and all tissue chambers were then inoculated with p haemolytica. additional serum and chamber fluid samples were co ... | 1994 | 8191004 |
| antigenic composition of pasteurella haemolytica serotype-1 supernatants from supplemented and nonsupplemented media. | supernatant preparations containing antigens produced by pasteurella haemolytica serotype 1 grown in nonsupplemented rpmi 1640 medium and 3 grown in supplemented rpmi 1640 media were evaluated. antigens were detected by immunoblotting each supernatant preparation with sera from 20 cattle with various degrees of resistance to experimentally induced pneumonic pasteurellosis. antigen-antibody bands at 49 and 30 kd were correlated with resistance in all 4 media. a 66-kd antigen-antibody band was cor ... | 1994 | 8192256 |
| a 4-year survey of antimicrobial susceptibility trends for isolates from cattle with bovine respiratory disease in north america. | the antimicrobial susceptibility trends of bovine respiratory disease (brd) pathogens isolated from 1988 to 1992 were determined. a total of 880 isolates representing pasteurella haemolytica, pasteurella multocida, and haemophilus somnus were used in the study. overall, resistance to ampicillin, tetracycline, erythromycin, and sulfamethazine was frequently encountered among strains of p. haemolytica and p. multocida. ceftiofur, an extended-spectrum cephalosporin originally marketed in 1988 for t ... | 1994 | 8195385 |
| the bovine placentome in bacterial and mycotic abortions. | placentomes were extracted from the uteri of 22 aborted cows and examined to detect the cause of abortion; fetuses or fetal abomasal contents from 15 of the cows were also examined. firm diagnoses of pasteurella haemolytica, actinomyces pyogenes, listeria monocytogenes, bacillus licheniformis, aspergillus fumigatus or mortierella wolfii abortion were made in 11 cases. the histopathological lesions showed some correlation with the identity of the bacterium isolated; the lesions of mycotic abortio ... | 1994 | 8197693 |
| iron supply of pasteurella multocida and pasteurella haemolytica. | pasteurella multocida and pasteurella haemolytica do not produce hydroxamate- or phenolate type siderophores. however, transport- and utilization systems could be detected for the well known siderophores ferrioxamine b, e, g, rhizoferrin and the intermediate 2,3-dihydroxybenzoic acid by means of cross-feeding tests in both pasteurella species. enterobactin and ferrichrome did not feed any of the pasteurella strains tested. additionally, alpha-ketoacids and alpha-hydroxyacids such as pyruvic acid ... | 1994 | 8207666 |
| oscillatory measurements, blood gas analysis and clinical observations after intravenous clenbuterol administration in healthy and acutely pneumonic calves. | the effects of clenbuterol (ventipulmin, boehringer ingelheim) on respiratory functions were investigated in 6 calves aged 4-6 weeks prior to and after experimental infection with pasteurella haemolytica a1. on days 1-3 (prior to infection) and on days 7-9 (after infection), blood gas analysis, monofrequency forced oscillation techniques and clinical examinations (heart rate, respiratory rate) were conducted for 135 min after the intravenous administration of clenbuterol (0.8 microgram/kg body w ... | 1993 | 8212524 |
| variation of abscess formation in cattle after vaccination with a modified-live pasteurella haemolytica vaccine. | during the spring of the first year of a vaccine study, 57 of 238 calves (24%), in which modified-live pasteurella haemolytica vaccine (mlv) was injected twice, developed 1 or more abscesses. abscesses were not observed after multiple visual examinations of 437 calves given killed p haemolytica bacterin or placebo injections of similar adjuvants used in the vaccine and bacterin. calves that developed abscesses after the second injection of mlv weighed significantly (p < 0.05) less (on the basis ... | 1993 | 8214890 |
| cloning, sequencing and expression of a pasteurella haemolytica a1 gene encoding a purk-like protein. | a membrane protein antigen of pasteurella haemolytica a1 encoded on the recombinant plasmid pyfc13 is isolated and characterized. nucleotide sequence analysis of the insert dna in pyfc13 identified the gene mpa1, which codes a protein of approximately 45 kda without signal sequence. the deduced amino acids from the dna sequence are homologous to bacillus subtilis purk by 29.4%; to schizosaccharomyces pombe pur6 by 29.34%, to saccharomyces cerevisiae pur6 by 25.867%; and to e. coli purk by 25.223 ... | 1993 | 8219279 |
| molecular analysis of the leukotoxin determinants from pasteurella haemolytica serotypes 1 to 16. | all sixteen serotypes of pasteurella haemolytica were shown to produce a leukotoxin protein which is immunologically related to the well-characterized serotype 1 leukotoxin. all of the leukotoxins were weakly hemolytic and were able to bind to bl-3 target cells. the leukotoxin determinants were characterized by southern blot hybridization by use of the previously cloned serotype 1 determinant as the probe, and a number of distinct classes were identified. the leukotoxin determinants from serotyp ... | 1993 | 8225575 |
| multivariate chemosystematics demonstrate two groups of actinobacillus actinomycetemcomitans strains. | chemical analysis by us has indicated that actinobacillus actinomycetemcomitans is not a homogeneous species. the present study used chemometric methods and a multitude of chemical characters to examine this further. strains were characterized by cell sugar and fatty acid contents, lysis kinetics during edta and edta plus lysozyme exposure, methylene blue reduction, and api zym enzymatic assessment of whole cells and outer membrane vesicles/fragments. in total, 41 quantitative variables were ana ... | 1993 | 8233565 |
| drug needs of the sheep industry. | two areas of drug needs in the sheep industry are reviewed. the first is the need for prolificacy drugs such as those used outside the country. these drugs would encourage out-of-season lambing, increased lambs/per ewe during the traditional lambing season and synchronize estrus for easier management of lambing and increased use of artificial insemination for faster genetic improvement. the other area is drugs to treat or prevent major causes of disease, specifically abortion, neonatal diarrhea, ... | 1993 | 8236760 |
| sequence analysis of four new heat-shock genes constituting the hslts/ibpab and hslvu operons in escherichia coli. | sequences of four new heat-shock (hs) genes of escherichia coli organized into two operons were determined. the operon at 83 min specifies two proteins of 15.8 kda (hslt) and 16.1 kda (hsls), which are identical to ibpa and ibpb, respectively. expression of mrna from a sigma 32-dependent promoter of the hslts/ibpab operon is stimulated 30-75-fold upon temperature upshift. the transcription start point (tsp) is located at a g, 96 bp upstream from the aug start codon of hslt/ibpa. the deduced amin ... | 1993 | 8244018 |
| efficacy of pasteurella haemolytica subunit antigens in a goat model of pasteurellosis. | the effectiveness of pasteurella haemolytica biovar a, serovar 1 (ph1) subunit vaccines was tested in goats, using challenge exposure by transthoracic injection. twenty-two weanling male spanish goats were randomly allotted to 4 groups. six goats were given 2 transthoracic injections into the lung 18 days apart with live ph1 impregnated in agar beads (positive controls). six goats were not given injections (negative controls). five goats were given 2 transthoracic injections into the lung 18 day ... | 1993 | 8250389 |
| [in vitro adherence of pasteurella haemolytica to tracheal mucins and a tracheal epithelial cell preparation from cattle]. | outer membrane protein preparations (omp) of 11 pasteurella haemolytica field isolates of the serovars a1, a2, a11 and non-typable strains from cattle were extracted by n-lauryl-sarcosine sodium salt. capsular extracts were prepared by heat treatment. both preparations and whole cell suspensions bound to a preparation of an epithelial cell wall fraction of trachea and to tracheal mucus of cattle. binding was demonstrated by enzyme linked immunosorbent assay (elisa). distinct high binding values ... | 1993 | 8250825 |
| in-vitro antibacterial properties of tilmicosin against australian isolates of pasteurella multocida and pasteurella haemolytica from cattle. | | 1993 | 8257321 |
| epidemiological study of enzootic pneumonia in dairy calves in saskatchewan. | a field study involving 325 calves from 17 dairy herds in saskatchewan was conducted to determine the risk of enzootic pneumonia and to assess its association with a number of factors. two different case definitions of pneumonia were used in the analyses: the first was based on producers' treatment risk (case1) and the second was based on semimonthly clinical examinations of calves by the research veterinarian (case2). the risk of pneumonia based on case1 was 39% and on case2 was 29%. the measur ... | 1993 | 8269363 |
| purification and quantitative measurement of bovine serum amyloid-a. | bovine serum amyloid-a (b-saa) was purified from a pool of acute phase serum using hydrophobic interaction chromatography and gel filtration. serum was applied at a low salt concentration to a phenyl-sepharose column and saa was eluted with a gradient of 0 to 6 m guanidine-hcl. fractions containing saa were pooled, concentrated and further purified by gel filtration on superose-12. the concentration of saa in bovine serum was quantified by an indirect elisa using rabbit anti-human saa and horser ... | 1993 | 8284495 |
| microdilution antimicrobial susceptibilities of selected gram-negative veterinary bacterial isolates. | gram-negative bacterial isolates (635) obtained from routine submissions to the oklahoma animal disease diagnostic laboratory during 1983-1987 were tested for antimicrobial susceptibility. minimal inhibitory concentrations (mics) were determined for the following antimicrobials using commercially prepared microdilution assay materials: ampicillin, cephalothin, chloramphenicol, erythromycin, gentamicin, kanamycin, oxytetracycline, penicillin g, spectinomycin, sulfachlorpyridazine, sulfadimethoxin ... | 1993 | 8286452 |
| systemic pasteurella haemolytica infection as a rare sequel to avirulent live pasteurella haemolytica vaccination in cattle. | eleven cases of systemic pasteurella haemolytica infection in cattle were identified from routine diagnostic laboratory submissions during the falls of 1988, 1989, and 1991. all cases came with a history of recent vaccination with an avirulent live culture p. haemolytica product. nine of 11 cases involved cattle vaccinated between 2 and 18 days previously with this product. ten of 11 cases involved 182-227-kg beef calves that were vaccinated between september and november during routine processi ... | 1993 | 8286454 |
| the effects of tick-borne fever on the susceptibility of ovine polymorphonuclear cells to p. haemolytica cytotoxin. | tick-borne fever predisposes sheep to secondary infections by pasteurella haemolytica. polymorphonuclear (pmn) cells obtained from sheep 2 to 7 days after the onset of parasitaemia due to cytoecetes phagocytophila, the causative agent of tick-borne fever, were significantly more susceptible in vitro to the cytotoxin of p. haemolytica than pmn obtained from age-matched control sheep. | 1993 | 8300918 |
| combined enzyme and antimicrobial susceptibility profiles of caprine pasteurella haemolytica serovar 2 respiratory tract isolates. | the present investigation describes a novel method of demonstrating strain diversity among pasteurella haemolytica biovar a, serovar 2 (pha2) nasal turbinate isolates from a flock of 32 experimental goats during a naturally occurring outbreak of pasteurellosis. after a 21 day conditioning period in a feedyard, 51 pha2 isolates from 27 culture-positive goats were identified including 1 on day 22, 14 on day 25, 21 on day 39, and 15 on day 66. each pha2 isolate was evaluated for its enzyme activity ... | 1993 | 8306653 |
| tick-borne fever as a possible predisposing factor in septicaemic pasteurellosis in lambs. | | 1993 | 8310610 |
| serum antibody response to purified pasteurella haemolytica capsular polysaccharide in cattle. | capsular polysaccharide (cp) of pasteurella haemolytica, biotype a, serotype 1, was purified and combined with saline solution, aluminum hydroxide, and freund's incomplete (oil) adjuvant. three groups of calves were administered the various antigen preparations. the cp in saline preparation was also administered to 5 mature cows. second injections were given 4 weeks after the first. weekly obtained serum samples were analyzed for p haemolytica-specific antibody, using the indirect hemagglutinati ... | 1993 | 8323052 |
| analysis of tandem, multiple genes encoding 30-kda membrane proteins in pasteurella haemolytica a1. | a number of outer membrane proteins (omps), including a 30-kda protein, may be important in eliciting immunity to pasteurella haemolytica a1, the causative agent of bovine pneumonic pasteurellosis. to better understand the nature of the 30-kda antigen, several genes encoding this protein were sequenced. sequence analysis revealed that three separate genes encoding similar, yet distinct, versions of the 30-kda protein are tandemly arranged on the p. haemolytica a1 chromosome. the genes appear to ... | 1993 | 8335249 |
| distribution of intramuscularly administered erythromycin into subcutaneous tissue chambers before and after inoculation with pasteurella haemolytica. | distribution of erythromycin into subcutaneous tissue chambers was characterised pharmacokinetically and the effect of pasteurella haemolytica infection on the extent of penetration was studied. thermoplastic tissue chambers were implanted subcutaneously in the paralumbar fossae of six calves. thirty-five days after implantation, the tissue chamber distribution of intramuscularly administered erythromycin (30 mg kg-1) was studied. chambers were then inoculated with p haemolytica and the tissue c ... | 1993 | 8337485 |
| clinical and serological evaluation of a pasteurella haemolytica a1 capsular polysaccharide vaccine. | the purified capsular polysaccharide (cps) of pasteurella haemolytica a1 was examined for its ability to protect cattle from experimental challenge with logarithmic-phase p. haemolytica. several preparations of p. haemolytica antigens were utilized in the experiment including cps, log-phase p. haemolytica culture supernatant, p. haemolytica recombinant leucotoxin (rlkt) and various combinations of the above. cps alone or in combination with culture supernatant or rlkt elicited no protection; rat ... | 1993 | 8342324 |
| effects of pasteurella haemolytica culture supernate on bovine tracheal smooth muscle. | pasteurella haemolytica leukotoxin is a ruminant specific leukotoxin that has been implicated in the pathogenesis of shipping fever in cattle. the present study was undertaken to determine the effect of this toxin on bovine airway smooth muscle. in vitro, the addition of culture supernate containing leukotoxin to bovine tracheal smooth muscle resulted in contraction of 55% of the muscle strips tested. maximum responses were reached rapidly during cumulative additions of this material. in 95% of ... | 1993 | 8358681 |
| expression of the pasteurella haemolytica leukotoxin is inhibited by a locus that encodes an atp-binding cassette homolog. | multicopy and single-copy chromosomal fusions between the pasteurella haemolytica leukotoxin regulatory region and the escherichia coli beta-galactosidase gene have been constructed. these fusions were used as reporters to identify and isolate regulators of leukotoxin expression from a p. haemolytica cosmid library. a cosmid clone, which inhibited leukotoxin expression from multicopy and single-copy protein fusions, was isolated and found to contain the complete leukotoxin gene cluster plus addi ... | 1993 | 8359916 |
| the region of human transferrin involved in binding to bacterial transferrin receptors is localized in the c-lobe. | iron-saturated human transferrin was digested with either chymotrypsin or trypsin to produce c-lobe and n-lobe protein fragments. individual protein fragments were purified by a combination of gel filtration and concanavalin a affinity chromatographic procedures. the c-lobe and n-lobe fragments of human transferrin were then used in binding assays to assess their ability in binding to the bacterial transferrin receptors. competitive binding assays demonstrated that the c-lobe fragment of human t ... | 1993 | 8361358 |
| vaccine-derived pasteurella haemolytica alter the responses of ovine pulmonary artery and vein to drugs acting on adenylate cyclase. | sheep were vaccinated with a live, vaccine-derived strain of pasteurella haemolytica. the ex vivo response of isolated pulmonary artery and vein to isoproterenol, cholera toxin, sodium fluoride and calcium were examined three days after vaccination. in the pulmonary artery (endothelium intact), vaccination did not alter the response to isoproterenol, or sodium fluoride whereas the relaxation response to cholera toxin was impaired. in the pulmonary artery (endothelium removed), the maximum relaxa ... | 1993 | 8378617 |
| use of dna analysis of pasteurella haemolytica biotype t isolates to monitor transmission in bighorn sheep (ovis canadensis canadensis). | pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of rocky mountain bighorn sheep (ovis canadensis canadensis) in central idaho. pasteurella haemolytica was isolated from five adult rocky mountain bighorn ewes captured from a free-ranging herd in central idaho. the lambs from two of these ewes delivered by cesarean section were free of p. haemolytica until 40 days of age and after repeated contact with their dams. the lambs subsequently developed signs of p ... | 1993 | 8385150 |
| vaccination with live pasteurella haemolytica alters vascular adrenergic reactivity. | rats were injected with sterile saline (controls) or 0.7 x 10(5) c.f.u. of pasteurella haemolytica (biotype a) obtained from a commercial vaccine. three days following vaccination animals were killed and aortas removed from the heart base. isolated aorta was placed in a tissue bath and the biophysical responses to phenylephrine (alpha-1 agonist), clonidine (alpha-2 agonist) and isoproterenol (beta agonist) determined. the results indicate that the alpha-2 adrenergic contractile response to cloni ... | 1993 | 8386846 |
| enhancement of neutrophil-mediated injury to bovine pulmonary endothelial cells by pasteurella haemolytica leukotoxin. | in this study, we used an in vitro coculture system to determine which virulence factor from pasteurella haemolytica a1 was responsible for augmenting bovine polymorphonuclear neutrophil (pmn)-mediated killing of bovine pulmonary artery endothelial cells (bpaec). a 51cr release cytotoxicity assay was used as a measure of bpaec killing. the mechanisms associated with this bpaec killing were also studied. our results demonstrated that the leukotoxin and not the lipopolysaccharide from p. haemolyti ... | 1993 | 8388866 |
| effect of copper deficiency on tissue, blood characteristics, and immune function of calves challenged with infectious bovine rhinotracheitis virus and pasteurella hemolytica. | fourteen holstein steers, averaging 30 d of age, were fed a semipurified diet (1.5 mg of cu/kg) supplemented with 0 (-cu) or 10 mg of cu/kg of diet (+cu) for 5 mo. calves were then challenged by consecutive exposure to aerosol preparations of infectious bovine rhinotracheitis virus (ibrv) and pasteurella hemolytica on d 0 and 7, respectively, of the 30-d study. serum ceruloplasmin and plasma copper were higher in +cu calves throughout the challenge period and increased in +cu calves after microb ... | 1993 | 8389346 |
| characterization of neuraminidases produced by various serotypes of pasteurella haemolytica. | neuraminidases produced by 16 strains of pasteurella haemolytica (serotypes 1 to 16) were characterized by molecular weight, antigenic identity, and substrate specificity. after growth in a chemically defined medium, stage i (lyophilized) culture supernatants were assayed for activity with n-acetylneuramin lactose, human alpha-1-acid glycoprotein, fetuin, colominic acid, and bovine submaxillary mucin. neuraminidase produced by p. haemolytica serotype a1 (ph a1) was purified by a combination of s ... | 1993 | 8406865 |
| three contiguous lipoprotein genes in pasteurella haemolytica a1 which are homologous to a lipoprotein gene in haemophilus influenzae type b. | an escherichia coli clone carrying the recombinant plasmid pph24 has been found to express highly immunoreactive antigens of pasteurella haemolytica a1. two or three antigens of approximately 30 kda were located to both the inner and outer membranes of the e. coli clone and in p. haemolytica a1. from the insert dna of 8.2 kbp on pph24, a fragment of 4.6 kbp was found to code for these antigens. nucleotide sequence analysis of the 4.6-kbp dna identified three genes (designated plpa, -b, and -c) a ... | 1993 | 8406866 |
| structural and serological specificities of pasteurella haemolytica lipopolysaccharides. | lipopolysaccharides (lpss) from 16 serotypes of pasteurella haemolytica were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and examined by silver staining and immunoblotting. silver staining of proteinase k-digested cell lysates revealed two rough lps serotypes (serotypes 2 and 8), which lacked demonstrable o-polysaccharide, while 14 others demonstrated a ladder pattern characteristic of smooth-type lps. purified lpss from several serotypes yielded o-polysaccharide in ad ... | 1993 | 8418039 |
| neuraminidase production by a pasteurella haemolytica a1 strain associated with bovine pneumonia. | the properties of an extracellular neuroaminidase produced by a pasteurella haemolytica a1 strain (isolated from a case of bovine pneumonia) during growth in a defined medium were examined in this investigation. this enzyme, isolated from concentrated culture supernatants of p. haemolytica a1, was active against n-acetylneuramin lactose, human alpha 1-acid glycoprotein, fetuin, and bovine submaxillary mucin. neuraminidase production paralleled bacterial growth in a defined medium and was maximal ... | 1993 | 8418046 |
| identification of pasteurella haemolytica a1 isolates from market-stressed feeder calves by use of enzyme and antimicrobial susceptibility profiles. | an epidemiologic study of pasteurella haemolytica serovar 1 (ph1) in market-stressed feeder calves from 7 farms in eastern tennessee was conducted. the nasal mucus of each calf was cultured sequentially at the farm of origin (day 0), at an auction market (day 133), and at a feedyard in texas (days 141, 148, 155, and 169). of the 103 calves tested, 77 were culture-positive, including 1 on day 0, 1 on day 133, 20 on day 141, 57 on day 148, 50 on day 155, and 14 on day 169. from the 143 ph1 isolate ... | 1993 | 8427478 |
| serum levels of tumor necrosis factor-alpha in calves experimentally infected with pasteurella haemolytica a1. | the purpose of this study was to document the levels of tumor necrosis factor-alpha (tnf) in serum of calves experimentally infected intratracheally with pasteurella haemolytica a1 and to determine if elevated tnf levels correlate with development of pneumonic pasteurellosis in the bovine. serum samples were collected at sequential time periods from 0 h to 72 h post inoculation with p. haemolytica. tnf levels in those sera were measured by a cytotoxicity assay utilizing the tnf-sensitive wehi 16 ... | 1993 | 8430501 |
| pasteurella haemolytica cytotoxin-dependent killing of neutrophils from bighorn and domestic sheep. | peripheral blood neutrophils from rocky mountain bighorn sheep (ovis canadensis canadensis) and domestic sheep were exposed to culture supernatants from pasteurella haemolytica isolates recovered from these two sheep species. six culture supernatants from bighorn sheep isolates and two from domestic sheep isolates were tested for cytotoxicity as determined by the release of lactate dehydrogenase. two of the bacterial culture supernatants from bighorn sheep were not cytotoxic, while the other fou ... | 1993 | 8445788 |
| classification, pathogenicity, and drug susceptibility of hemolytic gram-negative bacteria isolated from sick or dead chickens. | fifteen hemolytic gram-negative bacteria were isolated from the respiratory tracts of sick birds suffering from a long-lasting respiratory syndrome or from the bone marrow of dead birds distributed in the southern part of taiwan. these were classified as pseudomonas aeruginosa (10 isolates), pseudomonas fluorescens (2 isolates), pseudomonas stutzeri (1 isolate), pasteurella haemolytica (1 isolate), and proteus morganii (1 isolate). each isolate was inoculated intraperitoneally into one group of ... | 1993 | 8452510 |
| genomic fingerprinting of "haemophilus somnus" isolates by using a random-amplified polymorphic dna assay. | the random-amplified polymorphic dna (rapd) assay was used to generate dna fingerprints for 16 isolates of "haemophilus somnus," and one isolate each of "haemophilus agni," "histophilus ovis," "actinobacillus seminis," pasteurella haemolytica, and escherichia coli. the rapd assay differentiated among "h. somnus" isolates, which shared similarity coefficients of 0.46 to 1.00 on the basis of pairwise comparisons of rapd markers produced with nine random decamer primers. three virulent encephalitic ... | 1993 | 8458944 |
| further characterization of pasteurella haemolytica-like bacteria isolated from swine enteritis. | dna-dna hybridization studies were conducted on six pasteurella haemolytica-like (phl) organisms recovered from cases of swine enteritis. chromosomal-enriched fractions of phl organisms served as the source of dna for southern blots or as whole-chromosomal dna probes. under stringent hybridization conditions, chromosomal dna probes of a prototype phl (strain 6213a) organism distinguished other phl organisms from pasteurella haemolytica types a1 and t3, pasteurella multiocida types a:1 and a:3, e ... | 1993 | 8460470 |
| absence of circulating tnf alpha in experimental bovine pneumonic pasteurellosis. | | 1993 | 8470349 |
| a review of the toxicology of the antibiotic micotil 300. | micotil 300 is a new macrolide antibiotic for the treatment of bovine respiratory disease complex. as with other macrolides used in human and veterinary medicine, overdoses of micotil do not produce pathognomonic lesions. the toxicity dose response varies among laboratory animal and domestic livestock species. however, clinical evidence of micotil toxicity due to large doses is generally a manifestation of the positive chronotropic and negative inotropic cardiovascular effects. no adverse enviro ... | 1993 | 8470360 |
| morphological and functional disturbances in pulmonary vascular endothelium following exposure of sheep to an "avirulent" strain of pasteurella haemolytica. | sheep were vaccinated with a live attenuated strain of pasteurella haemolytica and killed 3 days later. segments of main intrapulmonic artery and vein were removed for biophysical and scanning electron microscopic studies. in the pulmonary artery, vaccination with pasteurella haemolytica caused an increase in the number of endothelial cell surface blebs and, in some cases, those blebs appeared to be splitting open, suggesting cell damage or irritation. there was a surprising lack of platelet adh ... | 1993 | 8473560 |
| molecular characterization of a leukotoxin gene from a pasteurella haemolytica-like organism, encoding a new member of the rtx toxin family. | a pasteurella haemolytica-like organism, a new species of bacterium isolated from piglets with diarrhea, secretes a leukotoxin into the culture media. western blot (immunoblot) analysis indicated that this leukotoxin cross-reacted with antileukotoxin antibody derived from cattle immunized with p. haemolytica. five overlapping recombinant bacteriophages carrying the gene for this 105-kda polypeptide were identified with a dna probe containing sequences from the p. haemolytica lktca genes from a p ... | 1993 | 8478098 |
| functional replacement of the hemolysin a transport signal by a different primary sequence. | secretion of the 107-kda hemolysin a (hlya) from escherichia coli is mediated by the membrane proteins hemolysin b and hemolysin d. hemolysin b is a member of the so-called atp binding cassette transporter superfamily, which includes the multidrug resistance p-glycoprotein, the cystic fibrosis cftr protein, and the major histocompatibility complex-associated transporter of antigenic peptides. recognition of hlya by the hemolysin b/d transporter is dependent on a signal sequence mapped to the c-t ... | 1993 | 8483936 |
| the role of induced virulence factors produced by pasteurella haemolytica in the pathogenesis of bovine pneumonic pasteurellosis: review and hypotheses. | in the pathogenesis of pneumonic pasteurellosis, there is an abrupt commensal to pathogen shift from a predominance of p. haemolytica serotype 2 (st2) to serotype 1 (st1) in the bovine upper respiratory tract (urt) microfloral population. this occurs following periods of stress associated with development of this disease. data are reviewed from recent publications supporting the contention that surface-expressed st1-specific factor(s) could be critical in mediating urt adhesion and colonization. ... | 1993 | 8485643 |
| molecular analysis of the actinobacillus pleuropneumoniae rtx toxin-iii gene cluster. | actinobacillus pleuropneumonia strains that secrete three different exotoxins (apxi, apxii, and apxiii) have been implicated in the etiology of porcine pleuropneumonia. to understand the role of these toxins in the pathogenesis of this disease, we have previously reported the cloning of the hemolysin gene (apxii) (chang et al., 1989a), which encodes a 110-kd polypeptide with hemolytic and cytotoxic activity. to clone the third toxin gene (apxiii), a new genomic library using a. pleuropneumoniae ... | 1993 | 8494611 |
| evaluation of the rapid nh system for identification of haemophilus somnus, pasteurella multocida, pasteurella haemolytica, and actinobacillus pleuropneumoniae isolated from cattle and pigs with respiratory disease. | haemophilus somnus, pasteurella haemolytica, pasteurella multocida, and actinobacillus pleuropneumoniae from cattle and pigs with respiratory disease were used to evaluate the rapid nh system (innovative diagnostics, atlanta, ga.). minor modifications of the rapid nh system to include animal source and growth requirements would permit the identification of all isolates tested. | 1993 | 8501242 |
| comparison of protection of experimentally challenged cattle vaccinated once or twice with a pasteurella haemolytica bacterial extract vaccine. | three groups of calves (15-18 per group) were injected twice at a 3-week interval with 2 doses of phosphate buffered saline (pbs, control group), 2 doses of presponse, a pasteurella haemolytica a1 bacterial extract vaccine (presponse-2 group) or 1 dose of pbs followed by a 2nd vaccination with 1 dose of presponse (presponse-1 group). three weeks after the 2nd vaccination, the calves were challenged intratracheally with p. haemolytica a1. calves were evaluated clinically for 3 days prior to chall ... | 1995 | 8521349 |
| serum antibody responses of cattle to iron-regulated outer membrane proteins of pasteurella haemolytica a1. | serum antibody responses to the 70, 77, and 100 kda iron-regulated outer membrane proteins (iromps) of pasteurella haemolytica a1 were studied in cattle vaccinated with outer membrane protein (omp) enriched outer membrane fraction, iromp-enriched outer membrane fraction or live p. haemolytica. vaccination with an iromp-enriched outer membrane fraction stimulated antibodies to the 70 kda iromp, whereas vaccination with live p. haemolytica stimulated antibodies to the 70 and 77 kda iromps. in a se ... | 1995 | 8533287 |
| definition of chemiluminescence and superoxide production responses of bovine neutrophils to selected soluble and particulate stimulants, and comparisons with the responses to pasteurella haemolytica. | we defined methods for use of luminol-dependent chemiluminescence (ldcl) and superoxide anion (o2-) production as parameters of the oxidative metabolism of neutrophils isolated from 1.5- to 5-week-old neonatal calves. we determined how variations in blood sample handling, agonist preparation, individual variability, and age of calves influenced the ldcl and o2- responses to certain agonists, and defined concentrations of soluble and particulate agonists that maximally stimulated the oxidative me ... | 1995 | 8533976 |
| enzyme release by bovine neutrophils. | release of enzymes from cytoplasmic granules has been postulated to have a major role in neutrophil-mediated tissue injury. secretion or release of primary granules, specific granules, and cytosolic enzymes by bovine neutrophils was examined by quantifying the release of beta-glucuronidase, b12-binding protein, and lactate dehydrogenase, respectively, in response to predetermined amounts of phorbol myristate acetate, calcium ionophore, and opsonized zymosan. these responses were compared with th ... | 1995 | 8533977 |
| the influence of supplemental chromium and vaccines on the acute phase response of newly arrived feeder calves. | the acute phase response as indicated by serum haptoglobin and total haemolytic complement activity (ch50) was measured in 72 cross-bred steer calves purchased at sales in ontario. during the 28 day (d) trial, 18 steers were randomly assigned to each of the following groups: 1) control; 2) vaccinated (infectious bovine rhinotracheitis, parainfluenza-3, bovine viral diarrhea, bovine respiratory synctial virus vaccine plus pasteurella haemolytica vaccine); 3) supplemental chelated cr (0.14 mg/kg); ... | 1995 | 8548694 |
| isolation of an ovine pulmonary surfactant-associated anionic peptide bactericidal for pasteurella haemolytica. | ovine pulmonary surfactant is bactericidal for pasteurella haemolytica when surfactant and bacteria mixtures are incubated with normal ovine serum. to isolate this component, surfactant (1 mg/ml) was centrifuged at 100,000 x gav, and the supernatant was fractionated by hplc. fractions were eluted with acetonitrile (10-100%)/0.1% trifluoracetic acid and tested for bactericidal activity. amino acid and sequence analysis of three bactericidal fractions showed that fraction 2 contained h-gdddddd-oh, ... | 1996 | 8552650 |
| [main infectious agents involved in the etiology of lung diseases of small ruminants in northern cameroon]. | between 1990 and 1992, 91 necropsies of small ruminants affected with pulmonary illness led to the isolation of the following strains of mycoplasma (m.): m. mycoides subsp. mycoides lc, m. ovipneumoniae, m. agalactiae, m. sp. type d2 and m. arginini. eleven pasteurella multocida strains (serotypes a1, a3, a5, a7 and d2) and 11 pasteurella haemolytica strains (serotypes 1, 2, 3, 6, 7, 8 and 9) were isolated. corynebacterium pseudotuberculosis, actinomyces pyogenes, staphylococcus sp., streptococc ... | 1995 | 8552842 |
| serotypes of pasteurella multocida and pasteurella haemolytica isolated from pneumonic lesions in cattle and sheep from méxico. | a total of 13,000 pairs of lungs were examined at mexico's city abbatoir, where 8,000 corresponded to sheep and 5,000 to cattle. from those, 224 pneumonic lesions were observed, obtaining 97 positive isolates, which yielded 112 strains of pasteurella sp. forty isolates were identified as p. haemolytica and 72 as p. multocida. one-hundred percent of p. haemolytica belonged to biotype a. serotypes were determined by indirect haemagglutination. p. multocida isolates were classified according to the ... | 1995 | 8552871 |
| bronchopneumonia in mice caused by pasteurella haemolytica a2 after predisposition by ovine bordetella parapertussis. | initial intranasal inoculation of four to eight-week-old swiss white mice with 7.5 x 10(6) colony forming units (cfu) of ovine b. parapertussis followed 30 min, three or five days, by intranasal inoculation with 1.4 x 10(5) cfu of pasteurella haemolytica a2 resulted in a more severe infection pattern than when either agent was administered alone. histopathological examination showed that inoculation with b. parapertussis alone caused a bronchopneumonia the severity of which was dependant upon th ... | 1995 | 8560736 |
| evaluation of the nebulisation of sodium ceftiofur in the treatment of experimental pasteurella haemolytica bronchopneumonia in calves. | severe acute bronchopneumonia was induced in 24 conventional friesian holstein calves by inoculating them intratracheally with pasteurella haemolytica type a1. twelve of the calves were treated intramuscularly with sodium ceftiofur and 12 were treated with an aerosol of sodium ceftiofur. the mortality rate in the group of calves treated with the aerosol was significantly lower, and their clinical and haematological parameters returned to normal significantly faster than in the calves treated int ... | 1995 | 8588104 |
| increased tumor necrosis factor-alpha and interleukin-1 beta expression in the lungs of calves with experimental pneumonic pasteurellosis. | we used a well characterized pneumonic pasteurellosis model in calves to determine whether increased proinflammatory cytokines, tumor necrosis factor-alpha (tnf-alpha) and interleukin-1 beta (il-1 beta) expression and secretion were associated with pneumonic lesions. bronchoalveolar lavage fluids, lavage cells consisting of alveolar macrophages and neutrophils with degenerative changes, and lung tissues were analyzed for the presence of tnf-alpha and il-1 beta approximately 48 h following endobr ... | 1995 | 8588337 |