Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| serum antibody response of ewes and their lambs to pasteurella haemolytica. | vaccination of pregnant ewes with a pasteurella haemolytica adjuvanted vaccine which contained serotypes a1, a2 and a6 antigens caused significant increases in serum antibody titres to a1 and a6 measured by the indirect haemagglutination test (iha). the response to vaccination with the serotype a2 antigen contained in this vaccine cannot be measured by the iha test. there were also increased antibody titres in the colostrum from the vaccinated ewes and in the serum of their lambs after sucking c ... | 1980 | 7445357 |
| microorganisms associated with abscesses of sheep and goats in the south of iran. | a total of 86 abscesses (45 and 41) abscesses of sheep and goats, respectively) were examined for their causal agents; 44 of these abscesses were located subcutaneously, and the remaining 42 were in lungs, livers, intestines, and udders. a total of 23 different types of microorganisms were isolated from 78 abscesses; bacteria were not detected in the remaining eight abscesses. microorganisms isolated were: species of the genera corynebacterium, staphylococcus, and streptococcus and pasteurella, ... | 1980 | 6996545 |
| study of bovine pulmonary response to pasteurella haemolytica: specificity of immunoglobulins isolated from the bovine lung. | a procedure is described for the isolation and purification of the immunoglobulins (ig)a and igg from pulmonary lavage fluid from calves. these ig were isolated from calves experimentally exposed to pasteurella haemolytica via aerosolization and from nonexposed calves. the specificity of these fractions toward p haemolytica was examined, using an indirect fluorescent antibody test and agglutination reactions. specific antibody activity was detected in the iga and igg fractions from calves expose ... | 1980 | 7001963 |
| evidence of cytocidal effects of pasteurella haemolytica on bovine peripheral blood mononuclear leukocytes. | pasteurella haemolytica had a pronounced cytocidal effect on bovine mononuclear leukocytes. mononuclear leukocytes separated from venous blood on ficoll-diatrizoate and incubated with either p haemolytica or escherichia coli showed a significantly greater percentage cell death than did cells incubated with staphylococcus epidermidis or controls. neither heat-killed nor x-irradiated p haemolytica caused a significant percentage cell death. the cytotoxic potential of p haemolytica was dose related ... | 1980 | 7013577 |
| response of calves to lung challenge exposure with pasteurella haemolytica after parenteral or pulmonary immunization. | in 3 experiments, calves were vaccinated by intrabronchial or subcutaneous injection of formalinized pasteurella haemolytica. immunization was evaluated by assessing the clinical and pathologic responses to pulmonary challenge exposure with live homologous bacteria. serum and bronchoalveolar washings were titrated for antibody by indirect (antiglobulin) bacterial agglutination. response to challenge exposure was determined by increased rectal temperature and faster respiratory rate. in all exper ... | 1980 | 7212406 |
| the effect of vaccination of lambs with live parainfluenza virus type 3 on pneumonia produced by parainfluenza virus type 3 and pasteurella haemolytica. | 1980 | 6258113 | |
| study of bovine pulmonary response to pasteurella haemolytica: pulmonary macrophage response. | a resistance state was produced in the bovine lung by exposing calves to an aerosolized mist of pasteurella haemolytica. verification of the resistant state was determined by an increase in clearance of p haemolytica from lungs of exposed calves vs lungs from nonexposed calves over 24 hours. bovine pulmonary macrophages isolated from lung washings of calves exposed to p haemolytica and from lungs of nonexposed calves failed to phagocytize p haemolytica in vitro. whole serum, pulmonary immunoglob ... | 1980 | 7436096 |
| influence of bronchoalveolar washing supernatants and stimulated lymphocyte supernatants on uptake of pasteurella haemolytica by cultured bovine alveolar macrophages. | bovine alveolar macrophages cultured for 12 hours with normal bovine bronchoalveolar washing supernatants (bws) showed impaired phagocytosis of pasteurella haemolytica when compared with phagocytosis in control cultures. such impairment was not observed when another gram-negative bacillus, yersinia enterocolitica, was used. the bws from calves inoculated subcutaneously or intrabronchially with p haemolytica inhibited uptake of p haemolytica when bws were incubated with the bacteria prior to addi ... | 1980 | 7369621 |
| the possible role of respiratory syncytial virus and pasteurella spp in calf respiratory disease. | in a natural outbreak of respiratory disease during 1976, 31 of 43 calves showed moderately severe clinical signs which included pyrexia (maximum 42 degrees c) and tachypnoea. during the outbreak infection by respiratory syncytial virus (rsv) was demonstrated by isolation from nasopharyngeal swabs or by serology. pasteurella haemolytica or p multocida were isolated from the blood of four and five calves respectively. in the month before disease 65 per cent of the calves showed significant antibo ... | 1980 | 7210428 |
| demonstration of an r plasmid in a strain of pasteurella haemolytica isolated from feedlot cattle. | a strain of pasteurella haemolytica isolated from the lungs of feedlot cattle with pneumonia was shown to be resistant to ampicillin, tetracycline, and streptomycin. two small, nontransmissible plasmids were shown to be responsible, for tetracycline resistance, a 5.7 x 10(6) dalton plasmid, and for streptomycin resistance, a 5.5 x 10(6) dalton plasmid. ampicillin resistance passed by transformation to escherichia coli could not be shown conclusively to be on a plasmid. ampicillin resistance was ... | 1980 | 6989297 |
| pasteurella haemolytica infections in sheep. | summary pasteurella haemolytica causes two distinct disease syndromes in sheep. p. haemolytica biotype a causes septicaemia in young lambs and pneumonia in all ages of sheep. biotype t produces an acute systemic disease affecting principally the upper alimentary tract and lungs in young adult sheep. the bacteriology, epidemiology and clinical and necropsy findings of the two diseases are described and the current situation regarding their experimental reproduction and immunology is reviewed. | 1980 | 22039979 |
| identification of pasteurella multocida and pasteurella haemolytica by api 20e, minitek, and oxi/ferm systems. | fifty serotyped isolates each of pasteurella multocida and pasteurella haemolytica were tested on the api 20e strip (analytab products, plainview, n.y.), the oxi/ferm tube (roche diagnostics, nutley, n.j.), and the minitek system (bbl microbiology systems, cockeysville, md.). none of the rapid test systems reliable identified these organisms. with the api system, discrepancies between expected and actual results for the oxidase test and nitrate test frequently resulted in misidentification or no ... | 1981 | 7016894 |
| immunity to parainfluenza type 3 virus and pasteurella haemolytica in sheep. | 1981 | 6277166 | |
| abortion due to pasteurella haemolytica in a cow. | 1981 | 7248884 | |
| neuraminidase activity of pasteurella haemolytica isolates. | neuraminidase activities of pasteurella haemolytica cell suspensions were measured. type isolates of the 12 established serotypes and bovine and ovine field isolates were included in the study. neuraminidase activities ranged from 0 to 0.87 u per mg (dry weight) of p. haemolytica. activity levels among the isolates studied were serotype associated. | 1981 | 7251161 |
| failure of a subunit bovine herpesvirus 1 vaccine to protect against experimental respiratory disease in calves. | in two experiments in which 31 calves were used, a bovine herpesvirus 1 subunit vaccine previously shown to elicit a strong immunological response in adult cattle failed to do so in younger animals and failed to protect against pneumonia caused by sequential exposure to virulent bovine herpesvirus 1 and pasteurella haemolytica aerosols. one of the experimental groups had been previously inoculated with a live commercial vaccine but even this failed to elicit a strong immunological response. thes ... | 1981 | 7272846 |
| [immunoprophylactic methods of prevention of pneumopathies in young cattle]. | the bivalent pasteurella vaccine used to immunise young cattle is an anaculture adsorbed to aluminium hydroxide and consisting of pasteurella multocida and pasteurella haemolytica serotypes which could be isolated; most often from pneumonically changed lungs and nasal mucus. it was administered together with trivalent antiviral vaccine and reduced by 50 per cent the loss rate due to mortality and emergency slaughtering. good protection was obtained from applying the first vaccination dose on the ... | 1981 | 7283646 |
| [identification of pasteurella serotypes by indirect hemagglutination]. | the indirect haemagglutination test was used for serological type identification of pasteurella strains. the pasteurella multocida strains were tested by parallel application of antigen production methods (common method plus hyaluronidase treatment). thirty-nine (72.2 per cent) of 54 pasteurella multocida strains, which had been obtained from cattle and swine, were serologically tested by the common technique of antigen production, their types being identified, while 42 (77.7 per cent) were test ... | 1981 | 7283648 |
| cytotoxic effects of pasteurella haemolytica on bovine neutrophils. | the interaction of logarithmic- and stationary-phase organisms of pasteurella haemolytica with bovine neutrophils was evaluated by an opsonocytophagic assay. only 5% to 8% of the logarithmic-phase p haemolytica 12296 organisms opsonized with normal bovine serum or antiserum were phagocytized. results from cytotoxicity assays, using the 51cr-release technique and the trypan blue exclusion test, indicated that the logarithmic-phase organisms liberated a soluble material that was cytotoxic to neutr ... | 1981 | 7294474 |
| pasteurella haemolytica in the bovine reproductive tract. | 1981 | 7343078 | |
| pathology of experimental infections with pasteurella haemolytica biotype t strain 4 in sheep. | 1981 | 7345108 | |
| interaction of pasteurella haemolytica with bovine neutrophils: identification and partial characterization of a cytotoxin. | timed cultures of pasteurella haemolytica 12296 strain in rpmi 1640 medium (with l-glutamine, ph 7.4) were used to determine the correlation between cytotoxin production and the age of the culture. cytotoxic activity was measured by a 51cr-release assay and trypan blue exclusion test with bovine neutrophils as target cells. results demonstrated that optimal cytotoxin production occurred during the logarithmic phase (peaked at 6 hours) and decreased during the stationary phase of bacterial growth ... | 1981 | 7039430 |
| purification and biological characterizationof endotoxin fractions from pasteruella haemolytica. | a sequential extraction procedure was used to provide 3 endotoxin fractions from pasteurella haemolytica with distinct biological and solubility properties. after acetone dessication, extraction with phenol, chloroform, and petroleum ether (2:5:8) provided a fraction designated rough lipopolysaccharide (lps). subsequent extraction of the cells with 45% phenol at 68 c yielded a fraction designated smooth lps, which was further divided into smooth precipitate and smooth supernatant, based on sedim ... | 1981 | 7041705 |
| inhibition of the blastogenic response of peripheral blood mononuclear cells to mitogen and antigens by bovine pulmonary macrophages and their culture supernatants. | bovine pulmonary macrophages were shown to inhibit blastogenic response of peripheral blood mononuclear cells to high and low level stimulation with phytohaemagglutinin p. the blastogenic response of cells from calves sensitised to pasteurella haemolytica or bcg, when stimulated with the corresponding antigen, was also suppressed by the addition of autologous pulmonary macrophages. twenty-four-hour-old macrophage culture medium was likewise inhibitory to the blastogenic response. determination o ... | 1981 | 7043684 |
| ovine systemic pasteurellosis caused by pasteurella haemolytica biotype t. | a detailed study was made of lambs aged 5--7 months naturally infected with pasteurella haemolytica biotype t. in addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. large numbers of p. haemolytica were present in the tonsil, oesophageal lesions, lung, liver and spleen, but few or none in other tissues. the evidence indicated that the disease was not a true septicaemia. it is ... | 1981 | 7463470 |
| [bacteriological diagnosis of haemophilus pleuropneumonia in swine]. | haemophilus pleuropneumonia (matthews and pattison, 1961; shope, 1964) should now be accepted as the valid name of the pathogen of hemophilus pleuropneumoniae of swine, according to studies and the description by kilian and co-workers (1978), while the name of haemophilus parahaemolyticus should be considered a synonym. on top of haemophilus pleuropneumoniae, haemophilus parasuis and haemophilus spec. "minor group" are also important to pneumonia, serositis, and arthritis (glässer's disease) in ... | 1981 | 7197147 |
| experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: immunofluorescent and electron microscopic studies. | colostrum-deprived lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. multiple tissues were examined by immunofluorescence to localize viral and bacterial antigens, and lungs were examined by electron microscopy for cytopathologic changes. using immunofluorescence, viral antigen was detected only in the respiratory tract, mainly in the bronchial and bronchiolar epithelium and in the alveolar wall. lesser amounts of vir ... | 1982 | 7046531 |
| cytotoxin of pasteurella haemolytica acting on bovine leukocytes. | the toxicity of pasteurella haemolytica culture supernatant for bovine and porcine cells was evaluated by 51cr release. sterile bacterial culture supernatant was toxic for bovine pulmonary lavage cells, peripheral blood lymphocytes and neutrophils, and cultured peripheral blood mononuclear cells, resulting in marked 51cr release. only slight release was induced from cultured madin-darby bovine kidney cells, porcine pulmonary lavage cells, peripheral blood mononuclear cells, lymphocytes, or neutr ... | 1982 | 7054131 |
| distribution of pasteurella haemolytica and pasteurella multocida in the bovine lung following vaccination and challenge exposure as an indicator of lung resistance. | experimental calves were vaccinated with virulent strains of pasteurella haemolytica or pasteurella multocida or with phosphate-buffered saline solution either by an aerosol method or by subcutaneous injection. calves were subsequently challenge exposed by intrapulmonic inoculation of the homologous virulent pasteurella species. sections obtained from the resulting pulmonic lesion were stained, using a fluorescent antibody technique, to determine relative number, location, and integrity of the c ... | 1982 | 7073058 |
| isolation and characterization of some previously unreported taxa from poultry with phenotypical characters related to actinobacillus-an pasteurella species. | cultural, morphologic, and biochemical characteristics of previously unreported taxa isolated from poultry and tentatively assigned to genus actinobacillus brumpt 1910 were compared to those of actinobacillus lignieresii, a equuli, a. seminis, a. suis, avian haemolytic actinobacillus sp., a.salpingitidis, avian pasteurella haemolytica-like strains, p haemolytica biovar t, p, ureae. p. multocida, p. pneumotropica, p. gallinarum and p. anatipestifer. evidence as obtained to indicate that taxon 1-- ... | 1982 | 7080825 |
| a new serotype (biotype t) of pasteurella haemolytica. | 1982 | 7089377 | |
| experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: pathologic studies. | nineteen 4-week-old, colostrum-deprived lambs were transtracheally inoculated with respiratory syncytial virus (rsv), pasteurella haemolytica biotype a serotype 1, or rsv and p haemolytica. pneumonic lesions were more frequent, more extensive, and more severe in lambs inoculated with rsv and p haemolytica than in lambs inoculated with either agent alone. lesions were seen in 2 of 4 lambs inoculated with p haemolytica alone, in 3 of 4 lambs inoculated with rsv alone, and in 11 of 11 lambs inocula ... | 1982 | 7091824 |
| experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: clinical and microbiologic studies. | four-week-old lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. when given in combination, rsv administration preceded p haemolytica by 3 or 5 days. lambs inoculated with p haemolytica or rsv developed a mild respiratory tract disease accompanied by a transient pyrexia in a few lambs. by 24 hours after inoculation of bacteria, all lambs inoculated with rsv and p haemolytica were listless, reluctant to move, and exhibi ... | 1982 | 7091825 |
| cytotoxic effect of pasteurella haemolytica on bovine polymorphonuclear leukocytes and impaired production of chemotactic factors by pasteurella haemolytica-infected alveolar macrophages. | pasteurella haemolytica exerted a cytotoxic effect on bovine polymorphonuclear neutrophil leukocytes. this effect was less than that seen with cultured alveolar macrophages or peripheral blood monocytes. when alveolar macrophages were cultured with pasteurella haemolytica, macrophages produced less chemotactic factors for polymorphonuclear leukocytes than did noninfected controls. this effect was reversible, in that removal of the bacteria permitted remaining macrophages to elaborate more chemot ... | 1982 | 7091828 |
| prevalence of pasteurella haemolytica serotypes isolated from ovine pasteurellosis in britain. | 1982 | 7112871 | |
| the pulmonary clearance of pasteurella haemolytica in calves infected with bovine virus diarrhea or mycoplasma bovis. | based on current literature which commonly associates bovine virus diarrhea virus and mycoplasma bovis with "pneumonic pasteurellosis," an investigation was conducted into the effect of these two pathogens on the capacity of bovine lung to clear inhaled pasteurella haemolytica. there was no significant effect (p less than 0.05) of either bovine virus diarrhea virus or m. bovis on the mean clearance rate of p. haemolytica, nor did the time interval of three, five or seven days between the first i ... | 1982 | 7127194 |
| a mouse model for estimation of pasteurella haemolytica deposition in calf lungs following aerosol exposure. | a method used to calculate the number of pasteurella haemolytica reaching the lungs of calves during an aerosol exposure is described. this method is based on a linear relationship of bacterial deposition in lungs of mice and calves when exposed to the same bacterial aerosol. | 1982 | 7127195 |
| serovars and biovars of pasteurella haemolytica isolated from calves. | 1982 | 7132018 | |
| middle ear infection in feedlot lambs. | of 133 feedlot lambs that died and were necropsied, 67 had normal lungs and 66 had pneumonic lungs. the middle ears of all lambs were opened by a specified technique. of the 67, 8 (12%) had otitis media, and of the 66, 26 (39%) had otitis media. the difference in prevalence in the 2 groups was highly significant (p less than 0.005). in acute stages, the mucosae of tympanic cavities and tympanic membranes were inflamed and the cavities contained fluid, whereas in later stages lumens were partiall ... | 1982 | 7141977 |
| bovine peripheral blood polymorphonuclear neutrophil chemotactic response to pasteurella haemolytica or zymosan-activated serum. | the chemotactic influence of pasteurella haemolytica and of products of its growth was evaluated in vitro, using bovine peripheral blood polymorphonuclear neutrophils (pmn) in an underagarose migration assay system. pasteurella haemolytica was cultured, quantitated, killed, and lysed by freeze-thawing. the pmn directional migration toward p haemolytica lysate was not significantly (p less than 0.9) different from spontaneous random pmn migration. also, there was no chemotactic effect of viable 6 ... | 1982 | 7149395 |
| serotypes of pasteurella haemolytica in sheep in the midwestern united states. | nasal secretions were collected from healthy lambs from flocks in iowa and the surrounding states for 2 consecutive years. the 1st year, 322 lambs from 61 flocks yielded 202 pasteurella haemolytica isolates, representing 9 established serotypes and 30 untypeable isolates. the 2nd year, 249 lambs from 49 flocks yielded 73 isolates, representing 9 established serotypes and 7 untypeable isolates. more isolates belonged to serotype 2 than to any other serotype. serotypes 3, 4, and 10 (members of bio ... | 1982 | 7181204 |
| a comparison of immunoferritin, immuno-enzyme and gold-labelled protein a methods for the localization of capsular antigen on frozen thin sections of the bacterium, pasteurella haemolytica. | the staphylococcal protein a-gold method was found to be superior to the enzyme-or ferritin-linked antibody techniques for locating capsular antigens on cryosections of pasteurella haemolytica, and its sensitivity was similar to the enzyme-linked antibody method. the sensitivity of conventionally fixed and embedded material and cryosections of heavily fixed, lightly fixed and unfixed material were shown to be similar under routine laboratory conditions. | 1982 | 6752106 |
| antibody titers to pasteurella haemolytica a1 in ontario beef cattle. | indirect bacterial agglutination titers to pasteurella haemolytica a1 were determined in serum, thoracic, pericardial, or peritoneal fluid from cattle necropsied as part of the bruce county beef project in 1979-80 and 1980-81. antibody titers were also assayed in serum from 84 calves on entry to feedlots in the fall of 1979. titers on entry were low compared to antibody levels at necropsy. cattle which died with pneumonia, in particular those dying of fibrinous pneumonia (shipping fever), had lo ... | 1982 | 6756619 |
| purification and partial characterization of a macrophage cytotoxin from pasteurella haemolytica. | a protein from pasteurella haemolytica that was highly immunogenic and toxic toward bovine alveolar macrophages was partially purified. when isolated from culture supernatants of p haemolytica serotype 1 or serotype 6, the protein reacted on ouchterlony immunodiffusion tests with antisera from 12 serotypes of p haemolytica, but did not cross-react with antisera to serotypes of p multocida. this indicated that the protein may be specific for p haemolytica. bacteria were grown in dialysis culture ... | 1982 | 6807147 |
| the pulmonary clearance of pasteurella haemolytica in calves given corynebacterium parvum and infected with parainfluenza-3 virus. | four control calves were aerosolized with parainfluenza-3 and one week later with pasteurella haemolytica. three calves were given corynebacterium parvum at a dose of 15 mg/m2 body surface area, infected with parainfluenza-3 virus one week later, and aerosolized with p. haemolytica two weeks after c. parvum injection. all calves were killed four hours after p. haemolytica exposure and the bacterial retention in the lung was determined. parainfluenza-3 viral infection did not exert any suppressiv ... | 1982 | 6280824 |
| a review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. | unanswered questions on the etiology and prevention of shipping fever pneumonia have allowed this disease to remain one of the most costly to the north american cattle industry. research in this area has indirected that while pasteurella haemolytica and, to a lesser extent, p. multocida are involved in most cases, they seem to require additional factors to help initiate the disease process. bovine herpes virus 1 has been shown experimentally to be one such factor. this review examines in some de ... | 1982 | 6290011 |
| aerosol vaccination of calves with pasteurella haemolytica against experimental respiratory disease. | three experiments were conducted on calves in which the efficacy of vaccination with live pasteurella haemolytica in aerosol was tested by challenge with sequential aerosol exposure to bovine herpesvirus 1 and p. haemolytica. neither single nor multiple aerosol vaccinations protected against the experimental disease. macroscopically recognizable rhinitis, tonsillitis, tracheitis and pneumonia occurred in both controls and vaccinates. in one experiment as many as three aerosol vaccinations with l ... | 1982 | 6290014 |
| respiratory disease in calves produced with aerosols of parainfluenza-3 virus and pasteurella haemolytica. | in four experiments, 22 calves were exposed to aerosols of parainfluenza-3 virus, followed by pasteurella haemolytica at intervals of three to 13 days. the purpose of each experiment was to study viral-bacterial interactions in the respiratory tracts. two experiments, in which the viral aerosols were diluted by the addition of air, produced sporadic temperature elevations while two experiments with undiluted viral aerosols produced consistent temperature elevations. diluted viral aerosols produc ... | 1982 | 6290015 |
| experimental infection of lambs with an adenovirus followed by pasteurella haemolytica. | 1982 | 6294963 | |
| bovine herpesvirus-1 vaccination against experimental bovine herpesvirus-1 and pasteurella haemolytica respiratory tract infection: onset of protection. | the onset of protection offered by intranasal vaccination with attenuated bovine herpesvirus-1 (bhv-1) was studied in 18 calves given a virulent bhv-1 aerosol challenge inoculum and an aerosol challenge exposure to pasteurella haemolytica. calves challenge exposed with virus 3, 7, 11, 15, or 19 days after vaccination and challenge exposed 4 days later with pasteurella haemolytica did not develop viral-bacterial pneumonia, whereas 2 of 3 control calves died of fibrinous bronchopneumonia 40 and 60 ... | 1982 | 6183996 |
| demonstration of age-dependent capsular material on pasteurella haemolytica serotype 1. | extracellular capsular material was demonstrated on early log-phase cells of pasteurella haemolytica serotype 1 by the fluorescent-antibody and several capsular staining techniques. the presence of this material was shown to be age dependent. wide capsules were demonstrable on cells from 2- to 12-h cultures, whereas cells from 16- to 22-h cultures had very little cell-associated capsular material. the maneval technique most clearly demonstrated the presence of capsules on cells from young (6-h) ... | 1982 | 6186688 |
| the effect of mycoplasma ovipneumoniae and pasteurella haemolytica on specific pathogen-free lambs. | 1982 | 6211466 | |
| effect of oxytetracycline therapy on experimentally induced pneumonic pasteurellosis in lambs. | two groups of 10, specific pathogen free lambs were injected with a long acting oxytetracycline preparation at a dose rate of 20 mg/kg either 24 hours before or 24 hours after exposure to an aerosol of pasteurella haemolytica. when compared with the response of similarly infected but untreated lambs, the effect of pretreatment was to postpone the appearance of clinical signs of pneumonia for four days and the deaths of five lambs for five to six days post infection, by which time seven untreated ... | 1982 | 6214889 |
| extraction of capsular material from pasteurella haemolytica. | water and phosphate-buffered saline solution suspensions of early log-arithmic phase cells of pasteurella haemolytica were incubated for 1 hour at various temperatures to remove capsular material with a minimum of cell lysis or death. criteria used to determine capsular removal included change in agglutinability of the organism and disappearance of an antigenic component by the fluorescent antibody test and the agar-gel diffusion technique. the capsular material could be removed in a saline solu ... | 1982 | 6817676 |
| oat cells in the pathology of ovine pneumonia-pleurisy. | light microscope observations on oat cells in the ovine pneumonia-pleurisy complex are presented. this study is based on the experimental production of the disease by viruses and pasteurella haemolytica. oat cells appeared only in necrotic lesions associated with large numbers of p. haemolytica in the pneumonic lung. it is suggested that oat cells originate from blood monocytes, which transform into the oat shape when developing in the necrotic, hypoxic environment created by p. haemolytica. the ... | 1982 | 16030837 |
| antimicrobial use in feedlot calves: its association with culture rates and antimicrobial susceptibility. | data were collected on anti-microbial usage and health problems, in beef feedlot calves. although the association between them was not significant, many feedlot owners did not use recommended dosages or duration of treatment and this may have led to a reduced recovery rate.injectable tetracyclines were the most frequent antimicrobial for primary treatment of sick calves; whereas chloramphenicol was selected most frequently when the primary treatment appeared to be ineffective. treatment with ant ... | 1983 | 6831306 |
| the prevalence and microbiology of pneumonia in a flock of lambs. | a flock of new zealand romney lambs on a property in hawkes bay was examined from august 1978 to june 1979. in november, the lambs in the flock were allocated to groups as follows: 600 lambs from which groups were selected for slaughter by commercial criteria each month (selected groups); 700 lambs which were subdivided into 8 groups (random groups), one of which was sent for slaughter each month; and 25 lambs to monitor serological responses to viral infections (surveillance group). at slaughte ... | 1983 | 16030927 |
| evaluation of a selective medium for isolation of haemophilus pleuropneumoniae. | crystal violet, lincomycin, spectinomycin and bacitracin were evaluated as selective agents in media for isolation of haemophilus pleuropneumoniae. no single antimicrobial agent or combination of two or more inhibited all non-haemophilus strains (escherichia coli, pasteurella haemolytica, pasteurella multocida, streptococcus faecalis, streptococcus equisimilis and staphylococcus aureus) without marked suppression of 16 h. pleuropneumoniae strains. a medium containing 1 micrograms/ml of crystal v ... | 1983 | 6230143 |
| antigenic relationships between the serotypes of pasteurella haemolytica demonstrable by enzyme-linked immunosorbent assay (elisa). | mice and rabbits were immunised with sodium salicylate extracts (sse) prepared from each of 12 serotypes of pasteurella haemolytica, and the antisera to each were used in cross-indirect haemagglutination (iha) tests and cross-enzyme-linked immunosorbent assays (elisa) to study antigenic relationships between the serotypes. an indirect micro-elisa demonstrated common antigenic relationships which were not apparent by iha. antisera from both species revealed considerable shared antigenicity betwee ... | 1983 | 6191428 |
| specificity of the enzyme-linked immunosorbent assay (elisa) for antibodies in the sera of specific pathogen-free lambs vaccinated with pasteurella haemolytica antigens. | pooled serum from specific pathogen-free (spf) lambs vaccinated with sodium salicylate extracted (sse) antigens of pasteurella haemolytica serotype a1 was shown to contain antibody to other a serotype sse antigens when tested by the enzyme-linked immunosorbent assay (elisa). specific antibody to serotype a1 sse antigens was demonstrated by absorption of the serum pool with heterologous serotype sse antigens. the type-specific antigens of serotypes a1 and a9 were prepared by phenol--water extract ... | 1983 | 6191429 |
| hemoglobin enhancement of experimental infection of mice with pasteurella haemolytica. | a 2% hemoglobin preparation injected into mice along with pasteurella haemolytica culture was found to be as good an enhancer of virulence as a 7% swine gastric mucin preparation. the hemoglobin preparation was easier to prepare and was less toxic than mucin for mice when injected intraperitoneally. | 1983 | 6194721 |
| susceptibility of pasteurella haemolytica to the bactericidal effects of serum, nasal secretions and bronchoalveolar washings from cattle. | several isolates of logarithmic-phase organisms of pasteurella haemolytica were shown to be sensitive to an antibody and complement-mediated killing mechanism in adult bovine serum. data suggested that the classical complement pathway was important in the induction of bactericidal activity of serum. sera from calves after colostrum feedings (post-colostral sera) killed only 30% of the bacteria in spite of the presence of high levels of antibodies against p. haemolytica. addition of post-colostra ... | 1983 | 6419445 |
| synergism between mycoplasma bovis and pasteurella haemolytica in calf pneumonia. | 1983 | 6612963 | |
| antibiotic resistant pasteurella haemolytica. | 1983 | 6612978 | |
| experimental immunisation of lambs against pneumonic pasteurellosis. | methods of immunising lambs against pneumonic pasteurellosis, caused by several serotypes of pasteurella haemolytica, were assessed in specific pathogen free lambs. lambs were vaccinated intramuscularly with sodium salicylate extract (sse) of p haemolytica, either alone or in combination with heat-killed organisms (hko). sse of p haemolytica type a1 protected vaccinated lambs against pneumonia resulting from challenge with the homologous serotype. sse of type a2 also provided some protection but ... | 1983 | 6622850 |
| isolation of pasteurella haemolytica and correlation with serum antibody response in clinically normal beef calves. | bacteria from the nasal cavity and trachea were cultured, and serum antibody titers determined for pasteurella haemolytica serotype 1 in 164 beef calves obtained from a closed herd on range pasture. at the first sampling, p. haemolytica serotype 1 was cultured from 16.4% of the calves. antibody titers were determined by a quantitative fluorimetric method and the mean titer was 9.5 +/- 5.8. fifty-seven randomly selected calves were used to study the correlation of serum antibody response and posi ... | 1983 | 6659314 |
| pasteurella haemolytica cytotoxin neutralizing activity in sera from ontario beef cattle. | a random sample of sera obtained from cattle necropsied as part of the bruce county beef project in 1980-81 was assayed for the ability to neutralize the cytotoxin of pasteurella haemolytica a1. cattle dying of fibrinous pneumonia had significantly lower neutralizing activity in serum than cattle which died for reasons other than pneumonia. activity in pneumonic cattle was also lower than the mean of twelve samples randomly chosen from sera of cattle bled on entry to feedlots in the fall of 1979 ... | 1983 | 6667433 |
| isolation of satratoxins from the bedding straw of a sheep flock with fatal stachybotryotoxicosis. | during a period of several weeks, more than 100 sheep died at a hungarian farm. the animals exhibited fleece loosing, and hemorrhaging was the most important autopsy finding. pasteurella haemolytica was cultured from various organs. the bedding straw was abundantly covered with stachybotrys atra, and removal of the straw stopped the disease. methanol extraction of the bedding straw followed by solvent partitioning, column chromatography, preparative thin-layer chromatography, and high-pressure l ... | 1983 | 6683481 |
| effect of viral dose on experimental pneumonia caused by aerosol exposure of calves to bovine herpesvirus 1 and pasteurella haemolytica. | the effect of various aerosol doses of bovine herpesvirus 1, followed four days later by aerosol exposure to a constant level of pasteurella haemolytica, was studied in 16 crossbred hereford range calves. a collision nebulizer was used to generate aerosols from virus suspensions with concentrations of 10(8.2) (high), 10(5.2) (moderate) or 10(2.2) (low) tcid50/ml. the bacterial suspension contained 10(7) colony forming units/ml. control calves exposed only to p. haemolytica developed no pulmonary ... | 1983 | 6299485 |
| lack of virus-specific bacterial adherence to bovine embryonic lung cells infected with bovine parainfluenza virus type 3. | infection of bovine embryonic lung cells with bovine parainfluenza virus type 3 did not induce in vitro, virus-specific, hemadsorption-related adherence of corynebacterium pyogenes, haemophilus somnus, staphylococcus aureus, streptococcus zooepidemicus, pasteurella haemolytica, listeria monocytogenes, escherichia coli, pasteurella multocida, brucella sp., or salmonella typhimurium. | 1983 | 6302133 |
| histological changes in the respiratory tract of calves exposed to aerosols of bovine herpesvirus 1 and pasteurella haemolytica. | histopathological changes in the respiratory tract of 19 calves exposed sequentially to aerosols of bovine herpesvirus 1 (infectious bovine rhinotracheitis virus) and pasteurella haemolytica, either 4 or 5 days apart, are described. tissues were examined 1.5 to 14 days following the bacterial aerosol. the salient features were hyperaemia of pharyngeal tonsils and lamina propria of air passages and alveoli; alveolitis associated with oedema and fibrin in lumen and septa; pneumocytes, mononuclear ... | 1983 | 6302139 |
| susceptibility of bovid herpesvirus 1 to antiviral drugs: in vitro versus in vivo efficacy of (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the relative efficacies of a variety of antiviral drugs against bovid herpesvirus 1 was investigated. (e)-5-(2-bromovinyl)-2'-deoxyuridine and trifluorothymidine were found to be inhibitory at doses of 0.01 micrograms/ml in in vitro yield reduction and plaque reduction assays. in contrast, acylovir was inactive even at concentrations as high as 1,000 micrograms/ml. other drugs, including phosphonoformic acid, 9-beta-d-arabinofuranosyladenine, 5-iodo-2-deoxyuridine, and 1-beta-d-arabinofuranosylc ... | 1983 | 6307134 |
| the effect of dose, route and virulence of bovine herpesvirus 1 vaccine on experimental respiratory disease in cattle. | three experiments were conducted with calves in which, following intramuscular or intranasal vaccination with virulent or attenuated bovine herpesvirus 1, calves were protected against bovine herpesvirus 1 -- pasteurella haemolytica challenge. calves receiving low doses of vaccine had lower levels of antibody and greater evidence of virus replication upon challenge than those receiving higher doses. in contrast 11/13 unvaccinated controls had fibrino-purulent pneumonia following challenge. the i ... | 1983 | 6309347 |
| nonspecific suppressive effect of bovine herpesvirus type 1 on bovine leukocyte functions. | the effect of bovine herpesvirus type 1 on the specific and nonspecific immune response of calves was examined. animals with or without prior aerosol exposure to pasteurella haemolytica serotype a1 were aerosol challenged with 10(8) pfu of virus. blood and serum samples were taken before and after virus challenge for determining cell-mediated, humoral, and neutrophil responses. a significant depression of the blastogenic responses to phytohemagglutinin, p. haemolytica, and pasteurella multocida ... | 1983 | 6311742 |
| challenge exposure of cattle vaccinated with a chemically altered strain of pasteurella haemolytica. | calves vaccinated with a chemically altered strain of pasteurella haemolytica and their nonvaccinated controls were challenge exposed intranasally with the cooper strain of infectious bovine rhinotracheitis virus. five days later, the calves were challenge exposed intratracheally with the p haemolytica type a1. calves that had been vaccinated with large, medium, or small doses of the chemically altered vaccinal strain of p haemolytica had various degrees of resistance to the experimental challen ... | 1983 | 6314851 |
| prevention of experimental bovine pneumonic pasteurellosis with an extract of pasteurella haemolytica. | a total of 36 calves were used in three experiments to test the efficacy of a potassium thiocyanate extract of pasteurella haemolytica in protecting against experimental pneumonia. in each of experiments a and b, 12 calves were divided into three equal groups. the first group was vaccinated with an aerosol of a potassium thiocyanate extract twice, two weeks apart; the second group was vaccinated subcutaneously once only with the same extract. the third group of calves in both experiments remaine ... | 1983 | 6315195 |
| viral-bacterial pneumonia in calves: duration of the interaction between bovine herpesvirus 1 and pasteurella haemolytica. | sixteen six to eight month old beef calves were exposed individually to a five minute aerosol of bovine herpesvirus 1, isolate 108. aerosol exposure to pasteurella haemolytica (biotype a, serotype 1) was administered individually for five minutes at either four, ten, 20 or 30 days after the virus. fibrinous pneumonia and pleuritis occurred in all four groups but were most extensive and severe in those exposed to the virus and bacterium four days apart (the positive controls). fibrinous pneumonia ... | 1983 | 6315196 |
| exposure of calves to aerosols of parainfluenza-3 virus and pasteurella haemolytica. | the present study was undertaken to investigate whether sequential exposure to aerosols of parainfluenza-3 virus followed by pasteurella haemolytica, or p. haemolytica followed by parainfluenza-3 virus, could lead to the production of pulmonary lesions in conventionally-raised calves. twenty male calves with low serum antibody titres to both organisms were placed in five equal groups. synergism of parainfluenza-3 virus and p. haemolytica was not demonstrated in any of the sequentially infected g ... | 1983 | 6320999 |
| a quantitative fluorometric assay for the measurement of antibody to pasteurella haemolytica in cattle. | a rapid, simple fluorometric method is described for measuring antibody to pasteurella haemolytica in sera of cattle. various antigen preparations were compared for the test including live, formalin-killed and phenol-killed p. haemolytica. a preparation composed of formalin-killed organisms from a 22 hour culture gave consistent results and was used in the studies. the test was reproduciable with percent coefficients of variation for fluorescent signal unit values on ten or more replicate sample ... | 1983 | 6339016 |
| pasteurella haemolytica pneumonia in weaned housed single suckled calves. | 1983 | 6344410 | |
| proteolysis of sialoglycoprotein by pasteurella haemolytica cytotoxic culture supernatant. | proteolytic enzyme activity releasing sialo glycopeptides from 3h-labeled human erythrocyte ghosts was detected in cytotoxic (leukotoxic) culture supernatants from 9 of 12 pasteurella haemolytica serotypes. microcrystalline cellulose thin-layer chromatograms of radioactive water-soluble products showed the following two radioactive peaks: a high-mobility minor peak (rf, 0.54 to 0.74), identified as sialic acid, and a low-mobility major peak (rf, 0.18 to 0.21), partially characterized as a trichl ... | 1983 | 6352504 |
| use of a fluorometric immunoassay to determine antibody response to pasteurella haemolytica in vaccinated and nonvaccinated feedlot cattle. | a retrospective study of the antibody response to pasteurella haemolytica was conducted by using sera from 368 feedlot cattle divided among five experiments. in three experiments, live vaccines or a bacterin were administered to some of the cattle and others were left as nonvaccinated controls. in two experiments, cattle were not vaccinated. clinical signs of disease with subsequent recovery developed in 48.0% of the cattle, and 10.3% of the cattle died. vaccination had no apparent effect on mor ... | 1983 | 6355165 |
| the usefulness of the api 20 e classification system in the identification of actinobacillus actinomycetem comitans, actinobacillus seminis and pasteurella haemolytica. | the prepuces of lambs aged 6--8 months and semen of 2 adult rams were found to be infected with gram negative, non-motile, non-haemolytic, pleomorphic bacilli. these organisms were compared with those of known strains of actinobacillus actinomycetem comitans. actinobacillus seminis and pasteurella haemolytica, using the api 20 e classification system. applying the principles of numerical taxonomy, the majority of suspected strains of a. seminis could be classified as a. actinomycetem comitans an ... | 1983 | 6355954 |
| solid-phase enzyme immunoassay of bovine antibody responses following immunization against and natural infection with pasteurella haemolytica. | a solid-phase enzyme immunoassay (eia) was developed in order to monitor bovine antibody responses following immunization against and natural infection with pasteurella haemolytica serotype a:1. non-ionic surfactants, used in many antibody eias to reduce non-specific immunoglobulin binding, had to be avoided because they inhibited specific binding of bovine antibodies to p. haemolytica antigens. calves were immunized with a kscn extract of p. haemolytica. subcutaneously immunized animals develop ... | 1983 | 6362177 |
| effect of bacterial dose on pneumonia induced by aerosol exposure of calves to bovine herpesvirus-1 and pasteurella haemolytica. | sixteen crossbred hereford range calves were used to study the effects of pasteurella haemolytica aerosols of various concentrations administered 4 days after they were exposed to a uniform aerosol of bovine herpesvirus-1 (bhv-1). all calves were given bhv-1 on day 0. on day 4, groups of 4 calves each were exposed to aerosols of p haemolytica generated from suspensions with the following concentrations: high (10(8)/ml)--group 1, moderate (10(5)/ml)--group 2, low (10(2)/ml)--group 3, or none (con ... | 1983 | 6830012 |
| difficulty in protecting lambs against pasteurella haemolytica biotype a with antiserum. | 1983 | 6836892 | |
| cross-reactivity of haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay. | the specificity and sensitivity of agglutination, complement fixation, and enzyme-linked immunosorbent assay (elisa) procedures in the detection of antibodies to haemophilus somnus was investigated. h. somnus rabbit immune sera were found to agglutinate pasteurella multocida, staphylococcus aureus, and haemophilus agni and, in some instances, also pasteurella haemolytica, salmonella dublin, streptococcus agalactiae, and corynebacterium pyogenes. in complement fixation tests with saline extracts ... | 1983 | 6841584 |
| the effect of copper deficiency on the resistance of mice to infection with pasteurella haemolytica. | mice adjudged copper deficient on the basis of significantly decreased blood and tissue copper content and superoxide dismutase activity, but generally showing no clinical signs of deficiency, were infected intraperitoneally with pasteurella haemolytica. in 3 separate experiments the ld50 for deficient animals was significantly depressed below that of copper-sufficient controls. furthermore, in mice surviving challenge at doses near to the ld50, spleen weights were significantly increased and bo ... | 1983 | 6841690 |
| cytotoxic effect of pasteurella haemolytica on ovine bronchoalveolar macrophages in vitro. | live pasteurella haemolytica a1 was shown to have a cytotoxic effect on suspensions of sheep bronchoalveolar macrophages. cytotoxic activity was also demonstrable in bacteria-free supernatants from suspensions containing p. haemolytica. heat-killed and ultra-violet killed organisms of p. haemolytica and live staphylococcus aureus were not toxic to sheep bam. these results suggest that a bacterial cell-free cytotoxin is produced by metabolically active p. haemolytica. guinea-pig peritoneal macrop ... | 1983 | 6845634 |
| pasteurella haemolytica cytotoxin: production by recognized serotypes and neutralization by type-specific rabbit antisera. | a sterile culture supernatant from each of the 12 recognized serotypes of pasteurella haemolytica was toxic to bovine alveolar macrophages when assayed by 51cr release. types appeared to differ in their ability to liberate cytotoxin, although this may have reflected strain variation rather than serotype-related differences. toxicity was partially neutralized by type-specific rabbit antisera, with neutralization of the homologous toxin being more effective than that of the heterologous serotype. | 1983 | 6869971 |
| prevalence of pasteurella haemolytica in transported calves. | in 2 different years, calves from individual farms were sampled by nasal swab for pasteurella haemolytica at the farm, at an auction barn, and at a feedyard after they had been transported 1,600 km. serum antibody titers were determined and p haemolytica isolates were serotyped. the frequencies of p haemolytica isolations were low at the farm, greater at the auction barn, and markedly high at the feedyard. serotype 2 was the predominant isolate at the farm, and serotype 1 was the predominant iso ... | 1983 | 6870030 |
| rapid indirect hemagglutination test for serotyping pasteurella haemolytica. | a modified indirect hemagglutination test which enabled pasteurella haemolytica to be serotyped in 3 h is described. there was 100% correlation with the conventional test for both the 15 prototype strains and the 50 field isolates. it was found that nonviable cultures could also be serotyped. | 1983 | 6885990 |
| anticytotoxin activity of bovine sera and body fluids against pasteurella haemolytica a1 cytotoxin. | toxin neutralizing activity of bovine sera and body fluids against pasteurella haemolytica type a1 cytotoxin was evaluated by 51cr release assay using bovine peripheral blood mononuclear leukocytes as the target cells. sera collected from precolostral calves did not exert anticytotoxin activity at 10(-1) or higher dilutions, whereas randomly selected complement fixing antibody-negative sera neutralized on average over 90% of cytotoxin activity at the 10(-1) dilution and less than 50% of the toxi ... | 1984 | 6372970 |
| a study for the differentiation of actinobacillus seminis, a. actinomycetem-comitans, histophilus ovis and pasteurella haemolytica. | by using well-defined techniques under optimum conditions it is possible adequately to define the biochemical characteristics of typical a. seminis strains. a. seminis can be distinguished from histophilus ovis on the latter's distinctive colony morphology, but it cannot be distinguished from actinobacillus actinomycetem-comitans. these organisms, however, can be differentiated from pasteurella haemolytica on serological grounds and the latter's greater pathogenicity for mice. it is appreciated, ... | 1984 | 6379546 |
| interaction of bovine respiratory syncytial virus and pasteurella haemolytica in the ovine lung. | the potential synergistic effect of bovine respiratory syncytial virus (rsv) and pasteurella haemolytica in the production of pneumonia after aerosol/intranasal infection of conventionally reared lambs was evaluated. a mild clinical response was observed in lambs given virus and/or bacteria. gross pulmonary lesions were seen in 3 of 6 lambs given rsv and then p haemolytica 3 or 6 days later, respectively (groups d and e), and in 1 lamb of 5 given virus and bacteria simultaneously (group g). gros ... | 1984 | 6383152 |
| experimental production of bovine pneumonic pasteurellosis. | pneumonic pasteurellosis has been reproduced in conventional, weaned, friesian-cross calves using a strain of pasteurella haemolytica biotype a, serotype 1 (p haemolytica a1) isolated from a pathologically confirmed incident of bovine pneumonic pasteurellosis. the major clinical findings were pyrexia, hyperpnoea, tachypnoea, nasal discharge and reduced appetite. fibrinous pneumonia was present in the lungs of animals at necropsy on days 2 and 3 after initial infection while by days 9 and 10 afte ... | 1984 | 6390589 |
| bovine pneumonic pasteurellosis: model for pasteurella haemolytica- and pasteurella multocida-induced pneumonia in cattle. | pneumonic lesions in calves were induced with pasteurella haemolytica or p multocida. the inoculum, consisting of a suspension of either organism, was administered by transthoracic intrapulmonic injection to 23 calves. three died of septicemia; the 20 remaining, killed 96 hours after inoculation, had an expanding unifocal pneumonia qualitatively comparable with that of acute pneumonic pasteurellosis (shipping fever). the concentration of bacteria that consistently produced a lesion was a 5-ml vo ... | 1984 | 6395732 |
| bovine pneumonic pasteurellosis: effect of vaccination with live pasteurella species. | experimental bovine pneumonic pasteurellosis was induced in beef calves by a transthoracic challenge exposure with pasteurella haemolytica serotype 1 or p multocida type 3. challenge exposure lesions were quantified by a lesion scoring system based on size and extension of lesions with larger scores assigned to the more severe lesions. calves inoculated with live pasteurella sp by aerosol or parenteral routes developed high serum antibody titers to the homologous organism, as determined by a qua ... | 1984 | 6395733 |
| bovine pneumonic pasteurellosis: effect of culture age of pasteurella haemolytica used as a live vaccine. | five experiments were conducted that compared aerosol immunization of calves with live pasteurella haemolytica from logarithmic (6 hour) or stationary (20 to 22 hour) phase cultures. calves were challenge exposed by transthoracic injection with p haemolytica. in 4 experiments, calves inoculated with 6-hour cultures had slightly lower mean lesion scores (indicating greater resistance to challenge exposure) than those inoculated with 20- to 22-hour cultures. high antibody titers, as detected by a ... | 1984 | 6395734 |
| effect of prior natural exposure to pasteurella haemolytica on resistance to experimental bovine pneumonic pasteurellosis. | the effect of prior natural exposure to pasteurella haemolytica, as determined serologically, was studied with respect to resistance to experimental pneumonic pasteurellosis in 20 calves from 3 experiments. resistance to challenge exposure was measured using a lesion-scoring system. as measured by a quantitative fluorometric immunoassay, naturally acquired serum antibody titers to the organisms were 0 to 228. there was a significant correlation (p less than 0.05) between high naturally acquired ... | 1984 | 6395739 |