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observations on the female reproductive cycles of captive asian yellow pond turtles (mauremys mutica) with radiography and ultrasonography.the annual reproductive cycle of 27 female mauremys mutica was observed by radiography and ultrasonography from april 2006 to august 2007. radiography was used to monitor clutch size and ultrasonography was used to monitor changes in the ovarian follicles. the follicles started to enlarge in september and became preovulatory in january. the mean maximum follicle diameter of ovulation was 18.30+/-1.44 mm, and ovulation occurred from march through august. eggs were laid between april and august. t ...201619688810
[effects of incubation temperature and substrate humidity on embryonic development of mauremys mutica].yellow pond turtle (mauremys mutica) eggs were incubated in vermiculite under nine combinations of temperature and humidity, i. e., 25 degrees c and -12 kpa, 29 degrees c and -12 kpa, 33 degrees c and -12 kpa, 25 degrees c and -150 kpa, 29 degrees c and -150 kpa, 33 degrees c and -150 kpa, 25 degrees c and -300 kpa, 29 degrees c and -300 kpa, and 33 degrees c and -300 kpa, aimed to study the effects of incubation temperature and its interaction with substrate humidity on the embryonic developmen ...201020387446
molecular characterization and expression analysis of vsig4 from the asian yellow pond turtle, mauremys mutica.v-set and immunoglobulin domain-containing protein 4 (vsig4), a member of the immunoglobulin superfamily, plays an important role in the immune system. this study isolated and characterized a cdna encoding vsig4 (mavsig4) from the asian yellow pond turtle (mauremys mutica). the mavsig4 cdna is 1840 bp long and contains an open reading frame of 1,182 bp that encodes a polypeptide of 372 amino acids. the genomic sequence of mavsig4 spans 7,682 bp, with six exons and five introns. the phylogenetic ...201222990954
the mitochondrial genomes of three lineages of asian yellow pond turtle, mauremys mutica.the complete mitochondrial genomes of three lineages (n, tw and s) of mauremys mutica are determined in this study. the total lengths of the mitogenomes were 16,758 bp for n, 16 500bp for tw, and 16 494bp for s. the nucleotide composition was 26.3-27% for t, 26.2-26.8% for c, and 33.8-33.9% for a. the genomes encoded 37 genes typically found in other vertebrates. three csbs were identified, and the csb1 were variable. a long tandem repeats of (ttattata) 30 were found in the control region of n m ...201626061338
genetic diversity and relationship of mauremys mutica and m. annamensis assessed by dna barcoding sequences.the mitochondrial dna cytochrome c oxidase subunit i gene (coi) has been used as an efficient barcoding tool for species identification of animals. in this study, the barcoding sequences were used to assess the genetic diversity and relationship of mauremy mutica and m. annamensis. four currently recognized groups of m. mutica were classified into two groups in this study, with 6% intergroup distances, the s group and the n group, consistent to the calling of "southern turtle" and "northern turt ...201626260182
multiple paternity in the cultured yellow pond turtles (mauremys mutica).as a result of hunting and habitat loss, wild populations of the yellow pond turtle, mauremys mutica, are decreasing. the international union for conservation of nature considers m. mutica to be an endangered species. all studied freshwater turtles have polyandrous mating with multiple paternity. to survey the mating strategies of m. mutica, 1year's genetic data of parents and all offspring in an artificially captive population were analyzed. two groups of multiplex pcr containing 16 microsatell ...201728619267
evolutionary conservation of transferrin genomic organization and expression characterization in seven freshwater turtles.serum transferrin (tf), encoding an iron-binding glycoprotein, has been revealed to play important roles in iron transportation and immune response, and it also has been demonstrated to be valuable for phylogenetic analysis in vertebrates. however, the evolutionary conservation, expression profiles and positive selection of transferrin genes among freshwater turtle species remain largely unclear. here, the genomic dna and coding sequences of transferrin genes were cloned and characterized in sev ...201830392910
molecular cloning of esr1, bmpr1b, and foxl2 and differential expressions depend on maternal age and size during breeding season in cultured asian yellow pond turtle (mauremys mutica).the reproductive capacity (egg size and egg number) of most of oviparous animals, including the asian yellow pond turtle (mauremys mutica), is constrained by the maternal age and body size, but the mechanism determining the maternal reproductive ability remains unclear. to disclose how maternal age and size affect reproductive ability of m. mutica, we first identified the full-length cdnas from estrogen receptor 1 (esr1), bone morphogenetic protein receptor 1b (bmpr1b), and forkhead box l2 (foxl ...201930922947
identification of snps and copy number variations in mitochondrial genes related to the reproductive capacity of the cultured asian yellow pond turtle (mauremys mutica).mitochondria function as an energy transfer organelle for cell metabolism with the energy being used for processes such as reproduction. to investigate whether mutations and copy number variations in mitochondrial genes are related to the reproductive capacity of the asian yellow pond turtle, mauremys mutica, there was exploration of the distribution frequency of 129 single nucleotide polymorphism loci of six mitochondrial genes, nd1, nd2, cox2, nd4, cytb andd-loop, of turtles from a relatively ...201931036307
intervention on immunodeficiency mice and structural identification of enzymatic peptides from mauremys mutica and cuora trifasciata.mauremys mutica (asian yellow pond turtle, ypt) and cuora trifasciata (chinese three-striped box turtle, tbt) are traditional chinese medicine. they possess many biological characteristics, such as immune-enhancement, anti-inflammatory, anti-cancer effects. they have been used as folk anti-cancer drugs in central and southern china for a long time. however, there was no reports of comparing the immune-enhancement effect of ypt and tbt, nor of identifying the structures of ypt peptides and tbt pe ...201931042594
comparative transcriptome analysis reveals the sexual dimorphic expression profiles of mrnas and non-coding rnas in the asian yellow pond turtle (meauremys mutica).noncoding rnas (ncrnas), including long ncrnas (lncrnas), circular rnas (circrnas) and micrornas (mirnas) have been extensively studied in biological processes such as disease development, cell proliferation but remained unclear in sex differentiation in organisms. in this study, the transcriptome profiles were comparatively analyzed between male and female gonads in mauremys mutica. a total of 8237 differentially expressed genes (degs), 9573 de lncrnas, 84 de circrnas and 665 de mirnas were ide ...202032389707
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