Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| genome-wide identification, characterization and classification of ionotropic glutamate receptor genes (iglurs) in the malaria vector anopheles sinensis (diptera: culicidae). | ionotropic glutamate receptors (iglurs) are conserved ligand-gated ion channel receptors, and ionotropic receptors (irs) were revealed as a new family of iglurs. their subdivision was unsettled, and their characteristics are little known. anopheles sinensis is a major malaria vector in eastern asia, and its genome was recently well sequenced and annotated. | 2018 | 29334982 |
| identification of carboxylesterase genes associated with pyrethroid resistance in the malaria vector anopheles sinensis (diptera: culicidae). | carboxylesterases (cces) are one of three large detoxification enzyme families. some cces are active on synthetic insecticides with ester structures. anopheles sinensis is an important malaria vector in eastern asia. this study identified and characterized the cce genes in the a. sinensis genome and determined cce genes associated with pyrethroid resistance using rna sequencing (rna-seq) and quantitative reverse transcription - polymerase chain reaction (qrt-pcr), in a. sinensis from anhui, chon ... | 2018 | 28731595 |
| asgdb: a specialised genomic resource for interpreting anopheles sinensis insecticide resistance. | anopheles sinensis is an important malaria vector in southeast asia. the widespread emergence of insecticide resistance in this mosquito species poses a serious threat to the efficacy of malaria control measures, particularly in china. recently, the whole-genome sequencing and de novo assembly of an. sinensis (china strain) has been finished. a series of insecticide-resistant studies in an. sinensis have also been reported. there is a growing need to integrate these valuable data to provide a co ... | 2018 | 29321052 |
| complete circularized genome sequences of four strains of elizabethkingia anophelis, including two novel strains isolated from wild-caught anopheles sinensis. | we provide complete circularized genome sequences of two mosquito-derived elizabethkingia anophelis strains with draft sequences currently in the public domain (r26 and ag1), and two novel e. anophelis strains derived from a different mosquito species, anopheles sinensis (ar4-6 and ar6-8). the genetic similarity of all four mosquito-derived strains is remarkable. | 2017 | 29167265 |
| experimental infections of mosquitoes with severe fever with thrombocytopenia syndrome virus. | severe fever with thrombocytopenia syndrome (sfts) is a newly identified emerging infectious disease, which is caused by a novel bunyavirus (termed sftsv) in asia. although mosquitoes have not been identified as the primary vectors, as revealed by epidemiological surveys, their role in transmitting this sftsv as a suspicious vector has not been validated. | 2017 | 28569189 |
| pigsties near dwellings as a potential risk factor for the prevalence of japanese encephalitis virus in adult in shanxi, china. | the increasing trend of adult cases of japanese encephalitis (je) in china, particularly in northern china, has become an important public health issue. we conducted an epidemiological investigation in the south of shanxi province to examine the relationships between mosquitoes, japanese encephalitis virus (jev), and adult je cases. | 2017 | 28592296 |
| biology, bionomics and molecular biology of anopheles sinensis wiedemann 1828 (diptera: culicidae), main malaria vector in china. | china has set a goal to eliminate all malaria in the country by 2020, but it is unclear if current understanding of malaria vectors and transmission is sufficient to achieve this objective. anopheles sinensis is the most widespread malaria vector specie in china, which is also responsible for vivax malaria outbreak in central china. we reviewed literature from 1954 to 2016 on an. sinensis with emphasis on biology, bionomics, and molecular biology. a total of 538 references were relevant and incl ... | 2017 | 28848504 |
| comparison of the tracheal systems of anopheles sinensis and aedes togoi larvae using synchrotron x-ray microscopic computed tomography (respiratory system of mosquito larvae using sr-μct). | mosquito-borne diseases, such as malaria, dengue fever, and zika virus, are serious global health issues. vector control may be an important strategy in reducing the mortality caused by these diseases. the respiratory system of mosquito larvae in the water has to inhale atmospheric oxygen as aquatic organisms. in this study, the three-dimensional (3d) structures of the dorsal longitudinal trunks (dlts) of the tracheal systems of anopheles sinensis and aedes togoi were compared using synchrotron ... | 2017 | 28497875 |
| comparative physical genome mapping of malaria vectors anopheles sinensis and anopheles gambiae. | anopheles sinensis is a dominant natural vector of plasmodium vivax in china, taiwan, japan, and korea. recent genome sequencing of an. sinensis provides important insights into the genomic basis of vectorial capacity. however, the lack of a physical genome map with chromosome assignment and orientation of sequencing scaffolds hinders comparative analyses with other genomes to infer evolutionary changes relevant to the vector capacity. | 2017 | 28583133 |
| genome-wide identification, characterization and evolution of cuticular protein genes in the malaria vector anopheles sinensis (diptera: culicidae). | thirteen cuticular protein (cp) families have been recognized in arthropods. in this study, 250 anopheles sinensis cp genes were identified and named based on genome and transcriptome sequences. they were classified into 10 families based on motifs and phylogenetic analyses. in 11 other insect species, nine had cp numbers > 150 while apis mellifera and tribolium castaneum had cp numbers less than 52. the cps of eight species occupied > 1.4% of the total genomic gene number, whereas in three spec ... | 2017 | 28544438 |
| singapore's anopheles sinensis form a is susceptible to plasmodium vivax isolates from the western thailand-myanmar border. | singapore has been certified malaria-free by the world health organization since november 1982. however, sporadic autochthonous malaria outbreaks do occur. in one of the most recent outbreaks of vivax malaria, an entomological investigation identified anopheles sinensis as the most probable vector. as metaphase karyotype studies divided an. sinensis into two forms, a and b, with different vector competence: the investigation of vector competence of an. sinensis found in singapore was thus pursue ... | 2017 | 29145859 |
| investigation on mosquito-borne viruses at lancang river and nu river watersheds in southwestern china. | during 2007 and 2010, an extensive entomological survey was performed to assess the distribution of mosquitoes and mosquito-borne arboviruses at lancang river and nu river watersheds in southwestern china. a total of 20,450 mosquitoes consisting 20 species was trapped and submitted 261 pools according to species and location. culex tritaeniorhynchus and anopheles sinensis were the most abundant species. eighty-seven isolates representing 11 virus species in 8 genera were obtained from 6 mosquito ... | 2017 | 29083983 |
| characterization of posa and posa-like virus genomes in fecal samples from humans, pigs, rats, and bats collected from a single location in vietnam. | porcine stool-associated rna virus (posavirus), and human stool-associated rna virus (husavirus) are viruses in the order picornavirales recently described in porcine and human fecal samples. the tentative group (posa and posa-like viruses: pplvs) also includes fish stool-associated rna virus (fisavirus) as well as members detected in insects (drosophila subobscura and anopheles sinensis) and parasites (ascaris suum). as part of an agnostic deep sequencing survey of animal and human viruses in v ... | 2017 | 28948041 |
| non-destructive analysis of the internal anatomical structures of mosquito specimens using optical coherence tomography. | the study of mosquitoes and analysis of their behavior are of crucial importance in the on-going efforts to control the alarming increase in mosquito-borne diseases. furthermore, a non-destructive and real-time imaging technique to study the anatomical features of mosquito specimens can greatly aid the study of mosquitoes. in this study, we demonstrate the three-dimensional imaging capabilities of optical coherence tomography (oct) for structural analysis of anopheles sinensis mosquitoes. the an ... | 2017 | 28817086 |
| extensive resistance of anopheles sinensis to insecticides in malaria-endemic areas of hainan province, china. | anopheles sinensis is one of the major malaria vectors and among the dominant species in hainan province, china. the resistance of an. sinensis to insecticides is an important threat to malaria control. however, few reports on insecticide resistance of an. sinensis were reported in this area. eight districts in hainan province were selected as the study areas. insecticide susceptibility bioassays were tested on wild-caught female mosquitoes of an. sinensis to 4% dichlorodiphenyltrichloroethane ( ... | 2017 | 28719327 |
| the antenna transcriptome changes in mosquito anopheles sinensis, pre- and post- blood meal. | antenna is the main chemosensory organ in mosquitoes. characterization of the transcriptional changes after blood meal, especially those related to chemoreception, may help to explain mosquito blood sucking behavior and to identify novel targets for mosquito control. anopheles sinensis is an asiatic mosquito species which transmits malaria and lymphatic filariasis. however, studies on chemosensory biology in female an. sinensis are quite lacking. here we report a transcriptome analysis of an. si ... | 2017 | 28715466 |
| preliminary characterization of putative structural cuticular proteins in the malaria vector anopheles sinensis. | the insect cuticle protects against environmental stresses such as insecticides, physical injury, dehydration and pathogenic microorganisms. structural cuticular proteins (cps) are the primary components of the cuticle, and are of interest for physiology-based pest management methods. anopheles sinensis cps are poorly characterised at present, and therefore we performed whole-genome sequence analysis and re-analysed available transcriptome data to identify potential insecticide resistance-associ ... | 2017 | 28646525 |
| anopheles vectors in mainland china while approaching malaria elimination. | china is approaching malaria elimination; however, well-documented information on malaria vectors is still missing, which could hinder the development of appropriate surveillance strategies and who certification. this review summarizes the nationwide distribution of malaria vectors, their bionomic characteristics, control measures, and related studies. after several years of effort, the area of distribution of the principal malaria vectors was reduced, in particular for anopheles lesteri (synony ... | 2017 | 28734898 |
| receptivity to malaria in the china-myanmar border in yingjiang county, yunnan province, china. | the re-establishment of malaria has become an important public health issue in and out of china, and receptivity to this disease is key to its re-emergence. yingjiang is one of the few counties with locally acquired malaria cases in the china-myanmar border in china. this study aimed to understand receptivity to malaria in yingjiang county, china, from june to october 2016. | 2017 | 29162093 |
| rdl mutations predict multiple insecticide resistance in anopheles sinensis in guangxi, china. | anopheles sinensis is a major vector of malaria in china. the gamma-aminobutyric acid (gaba)-gated chloride channel, encoded by the rdl (resistant to dieldrin) gene, is the important target for insecticides of widely varied structures. the use of various insecticides in agriculture and vector control has inevitably led to the development of insecticide resistance, which may reduce the control effectiveness. therefore, it is important to investigate the presence and distribution frequency of the ... | 2017 | 29183375 |
| three-dimensional structures of the tracheal systems of anopheles sinensis and aedes togoi pupae. | mosquitoes act as a vector for the transmission of disease. the world health organization has recommended strict control of mosquito larvae because of their "few, fixed, and findable" features. the respiratory system of mosquito larvae and pupae in the water has a weak point. as aquatic organisms, mosquito larvae and pupae inhale atmosphere oxygen. however, the mosquito pupae have a non-feeding stage, unlike the larvae. therefore, detailed study on the tracheal system of mosquito pupae is helpfu ... | 2017 | 28287649 |
| sybr green-based one step quantitative real-time polymerase chain reaction assay for the detection of zika virus in field-caught mosquitoes. | the monitoring of vectors is one of the key surveillance measures to assess the risk of arbovirus transmission and the success of control strategies in endemic regions. the recent re-emergence of zika virus (zikv) in the tropics, including singapore, emphasizes the need to develop cost-effective, rapid and accurate assays to monitor the virus spread by mosquitoes. as zikv infections largely remain asymptomatic, early detection of zikv in the field-caught mosquitoes enables timely implementation ... | 2017 | 28927458 |
| seasonal dynamics of anopheles species at three locations in the republic of korea. | weekly changes in adult anopheles species were monitored at camp humphreys (ch), ganghwa island (gh), and warrior base (wb), from may-october, 2009-2010 to explore the relationship between plasmodium vivax development and vector dynamics in the republic of korea (rok). adult females were trapped and dissected to determine parity for estimating longevity, mortality, and birthrate. a degree-day (dd) method was used to estimate the extrinsic incubation period (eip) of p. vivax and duration of the g ... | 2017 | 29125241 |
| zika virus isolated from mosquitoes: a field and laboratory investigation in china, 2016. | a field investigation of arboviruses was conducted in dejiang, guizhou province in the summer of 2016. a total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. the mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. the supernatant was incubated with mosquito tissue culture cells (c6/36) and mammalian cells (bhk-21) for virus isolation. the results indicated t ... | 2017 | 29058107 |
| sequencing and analysis of the complete mitochondrial genome in anopheles sinensis (diptera: culicidae). | anopheles sinensis (diptera: culicidae) is a primary vector of plasmodium vivax and brugia malayi in most regions of china. in addition, its phylogenetic relationship with the cryptic species of the hyrcanus group is complex and remains unresolved. mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, of which mitochondrial genome data of an. sinensis is not available. | 2017 | 28969698 |
| a reassessment of the artificial infection of three predominant mosquito species with plasmodium vivax in shandong province, china. | under certain ecological circumstances, pathogens are able to rapidly adapt to new vectors. the great capacity of plasmodium spp. to adapt to new anopheline mosquito vectors on different continents and the continuous ecological changes attributed to humans might promote their adaptation to culicine vectors, which are known to infect humans. based on our current knowledge, it is difficult to predict whether such adaptations will occur. this study was aimed to determine the infection susceptibilit ... | 2017 | 27681543 |
| identification of larvicidal constituents of the essential oil of echinops grijsii roots against the three species of mosquitoes. | the screening of chinese medicinal herbs for insecticidal principles showed that the essential oil of echinops grijsii hance roots possessed significant larvicidal activity against mosquitoes. the essential oil was extracted via hydrodistillation and its constituents were determined by gas chromatography-mass spectrometry (gc-ms) analysis. gc-ms analyses revealed the presence of 31 components, with 5-(3-buten-1-yn-1-yl)-2,2'-bithiophene (5-bbt, 27.63%), αterthienyl (α-t, 14.95%),1,8-cineole (5.5 ... | 2017 | 28134799 |
| genetic diversity and population structure of the primary malaria vector anopheles sinensis (diptera: culicidae) in china inferred by cox1 gene. | anopheles sinensis is a primary vector for plasmodium vivax malaria in most regions of china. a comprehensive understanding of genetic variation and structure of the mosquito would be of benefit to the vector control and in a further attempt to contribute to malaria elimination in china. however, there is only inadequate population genetic data pertaining to an. sinensis currently. | 2017 | 28183358 |
| scanning electron microscopy of antennal sensilla of the eight anopheles species of the hyrcanus group (diptera: culicidae) in thailand. | antennal sensilla were first investigated in the eight medically and veterinary important anopheles mosquito species (anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae (= anopheles lesteri), anopheles peditaeniatus, anopheles pursati, and anopheles sinensis) of the hyrcanus group in thailand, using scanning electron microscopy (sem). four types of sensilla, including sensilla chaetica (large and small), sensilla trichodea (sharp- and blunt-tipp ... | 2017 | 27752768 |
| suppression of laccase 2 severely impairs cuticle tanning and pathogen resistance during the pupal metamorphosis of anopheles sinensis (diptera: culicidae). | phenol oxidases (pos) catalyze the oxidation of dopa and dopamine to melanin, which is crucial for cuticle formation and innate immune maintenance in insects. although, laccase 2, a member of the po family, has been reported to be a requirement for melanin-mediated cuticle tanning in the development stages of some insects, whether it participates in cuticle construction and other physiological processes during the metamorphosis of mosquito pupae is unclear. | 2017 | 28376914 |
| erratum to: genetic diversity and population structure of the primary malaria vector anopheles sinensis (diptera: culicidae) in china inferred by cox1 gene. | 2017 | 28253892 | |
| discovery of flavivirus-derived endogenous viral elements in anopheles mosquito genomes supports the existence of anopheles-associated insect-specific flaviviruses. | the flavivirus genus encompasses several arboviruses of public health significance such as dengue, yellow fever, and zika viruses. it also includes insect-specific flaviviruses (isfs) that are only capable of infecting insect hosts. the vast majority of mosquito-infecting flaviviruses have been associated with mosquito species of the aedes and culex genera in the culicinae subfamily, which also includes most arbovirus vectors. mosquitoes of the anophelinae subfamily are not considered significan ... | 2017 | 28078104 |
| spatiotemporal epidemic characteristics and risk factor analysis of malaria in yunnan province, china. | malaria remains an important public health concern in china and is particularly serious in yunnan, a china's provincial region of high malaria burden with an incidence of 1.79/10(5) in 2012. this study aims to examine the epidemiologic profile and spatiotemporal aspects of epidemics of malaria, and to examine risk factors which may influence malaria epidemics in yunnan province. | 2017 | 28077125 |
| evaluation of isotope (32)p method to mark culex pipiens (diptera: culicidae) in a laboratory. | the aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope (32)p and determine the optimal concentration of it. | 2016 | 27308279 |
| the increasing importance of plasmodium ovale and plasmodium malariae in a malaria elimination setting: an observational study of imported cases in jiangsu province, china, 2011-2014. | following initiation of china's national malaria elimination action plan in 2010, indigenous malaria infections in jiangsu province decreased significantly. meanwhile imported plasmodium infections have increased substantially, particularly plasmodium ovale and plasmodium malariae. given the risk for malaria resurgence, there is an urgent need to understand the increase in imported p. ovale and p. malariae infections as china works to achieve national malaria elimination. | 2016 | 27604629 |
| key knowledge gaps for plasmodium vivax control and elimination. | there is inadequate understanding of the biology, pathology, transmission, and control of plasmodium vivax, the geographically most widespread cause of human malaria. during the last decades, study of this species was neglected, in part due to the erroneous belief that it is intrinsically benign. in addition, many technical challenges in culturing the parasite also hampered understanding its fundamental biology and molecular and cellular responses to chemotherapeutics. research on vivax malaria ... | 2016 | 27430544 |
| landscape genetic structure and evolutionary genetics of insecticide resistance gene mutations in anopheles sinensis. | anopheles sinensis is one of the most abundant vectors of malaria and other diseases in asia. vector control through the use of insecticides is the front line control method of vector-borne diseases. pyrethroids are the most commonly used insecticides due to their low toxicity to vertebrates and low repellency. however, the extensive use of insecticides has imposed strong selection pressure on mosquito populations for resistance. high levels of resistance to pyrethroid insecticides and various m ... | 2016 | 27108406 |
| spatial and space-time distribution of plasmodium vivax and plasmodium falciparum malaria in china, 2005-2014. | despite the declining burden of malaria in china, the disease remains a significant public health problem with periodic outbreaks and spatial variation across the country. a better understanding of the spatial and temporal characteristics of malaria is essential for consolidating the disease control and elimination programme. this study aims to understand the spatial and spatiotemporal distribution of plasmodium vivax and plasmodium falciparum malaria in china during 2005-2009. | 2016 | 27993171 |
| structural divergence of chromosomes between malaria vectors anopheles lesteri and anopheles sinensis. | anopheles lesteri and anopheles sinensis are two major malaria vectors in china and southeast asia. they are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. both species belong to the anopheles hyrcanus group, but the extent of genetic differences between them is not well understood. to provide an effective way to differentiate between species and to find useful marke ... | 2016 | 27887641 |
| peculiar liquid-feeding and pathogen transmission behavior of aedes togoi and comparison with anopheles sinensis. | female mosquitoes transmit various diseases as vectors during liquid-feeding. identifying the determinants of vector efficiency is a major scientific challenge in establishing strategies against these diseases. infection rate and transmission efficiency are interconnected with the mosquito-induced liquid-feeding flow as main indexes of vector efficiency. however, the relationship between liquid-feeding characteristics and pathogen remains poorly understood. the liquid-feeding behavior of aedes t ... | 2016 | 26839008 |
| twenty-five new viruses associated with the drosophilidae (diptera). | drosophila melanogaster is an important laboratory model for studies of antiviral immunity in invertebrates, and drosophila species provide a valuable system to study virus host range and host switching. here, we use metagenomic rna sequencing of about 1600 adult flies to discover 25 new rna viruses associated with six different drosophilid hosts in the wild. we also provide a comprehensive listing of viruses previously reported from the drosophilidae. the new viruses include iflaviruses, rhabdo ... | 2016 | 27375356 |
| first detection of candidatus rickettsia barbariae in the flea vermipsylla alakurt from north-western china. | vermipsylla is a genus of the family vermipsyllidae within the order siphonaptera of fleas. vermipsylla alakurt is mainly distributed in alpine pastoral areas of kazakhstan, mongolia, china and nepal, and infests sheep, yaks and horses, causing irritation, poor condition, anaemia and even death. however, to date, no rickettsial agents have been reported in v. alakurt. | 2016 | 27267467 |
| imbalanced hemolymph lipid levels affect feeding motivation in the two-spotted cricket, gryllus bimaculatus. | insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. adipokinetic hormone (akh) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. we recently demonstrated that rna interference (rnai)-mediated knockdown of the akh receptor (akhr) reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, gr ... | 2016 | 27144650 |
| epidemiological analysis of bovine ephemeral fever in 2012-2013 in the subtropical islands of japan. | bovine ephemeral fever (bef) is a febrile disease of cattle that is transmitted by arthropod vectors such as mosquitoes and culicoides biting midges. an outbreak of bef recently occurred in ishigaki island and surrounding islands that are located southwest of japan. in this study, an epidemiological analysis was conducted to understand the temporal and spatial characteristics of the outbreak. factors associated with the disease spread within ishigaki island were investigated by hierarchical baye ... | 2016 | 26956227 |
| sero-molecular epidemiology of japanese encephalitis in zhejiang, an eastern province of china. | sporadic japanese encephalitis (je) cases still have been reported in zhejiang province in recent years, and concerns about vaccine cross-protection and population-level immunity have been raised off and on within the public health sphere. genotype i (gi) has replaced giii as the dominant genotype in asian countries during the past few decades, which caused considerable concerns about the potential change of epidemiology characteristics and the vaccine effectiveness. the aim of this study was to ... | 2016 | 27560360 |
| genome sequencing and genetic characterization of culex flavirirus (cxfv) provides new information about its genotypes. | culex flavivirus (cxfv) is an insect-specific virus that is widely distributed and primarily infects mosquito species from the genus culex. its hosts include culex tritaeniorhynchus, culex quinquefasciatus, and anopheles sinensis mosquitoes. since its original identification, cxfv has been reported in several countries. despite the increasing number of reports on cxfv, little is known about its genomic characteristics. it is unclear whether the phylogenetic relationships between the strains are ... | 2016 | 27658621 |
| rickettsia felis, an emerging flea-borne rickettsiosis. | rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ... | 2016 | 27340613 |
| collagen cross-linking: insights on the evolution of metazoan extracellular matrix. | collagens constitute a large family of extracellular matrix (ecm) proteins that play a fundamental role in supporting the structure of various tissues in multicellular animals. the mechanical strength of fibrillar collagens is highly dependent on the formation of covalent cross-links between individual fibrils, a process initiated by the enzymatic action of members of the lysyl oxidase (lox) family. fibrillar collagens are present in a wide variety of animals, therefore often being associated wi ... | 2016 | 27876853 |
| challenges in and lessons learned during the implementation of the 1-3-7 malaria surveillance and response strategy in china: a qualitative study. | china has made great progress in malaria control over the last century and now aims to eliminate malaria by 2020. in 2012, the country launched its 1-3-7 surveillance and response strategy for malaria elimination. the strategy involves to case reporting within 1 day, case investigation within 3 days, and focus investigation and public health actions within 7 days. the aim of this study was to evaluate the challenges in and lessons learned during the implementation of the 1-3-7 strategy in china ... | 2016 | 27716398 |
| characteristics of imported malaria and species of plasmodium involved in shandong province, china (2012-2014). | malaria remains a serious public health problem in shandong province, china; therefore, it is important to explore the characteristics of the current malaria prevalence situation in the province. in this study, data of malaria cases reported in shandong during 2012-2014 were analyzed, and plasmodium species were confirmed by smear microscopy and nested-pcr. a total of 374 malaria cases were reported, 80.8% of which were reported from 6 prefectures. of all cases, p. falciparum was dominant (81.3% ... | 2016 | 27658591 |
| further evidence of increasing diversity of plasmodium vivax in the republic of korea in recent years. | vivax malaria was successfully eliminated from the republic of korea (rok) in the late 1970s but re-emerged in 1993. two decades later as the rok enters the final stages of malaria elimination, dedicated surveillance of the local p. vivax population is critical. we apply a population genetic approach to gauge p. vivax transmission dynamics in the rok between 2010 and 2012. | 2016 | 26990869 |
| bioactivities of a new pyrrolidine alkaloid from the root barks of orixa japonica. | a new pyrrolidine alkaloid named (z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one was isolated from the ethanol extract of the root barks of orixa japonica. the structure of the new alkaloid was elucidated on the basis of nmr and ms analysis. the compound exhibited larvicidal activity against the fourth instar larvae of aedes aegypti (lc50 = 232.09 μg/ml), anopheles sinensis (lc50 = 49.91 μg/ml), and culex pipiens pallens (lc50 = 161.10 μg/ml). the new alkaloid also poss ... | 2016 | 27918451 |
| assessing the impact of meteorological factors on malaria patients in demilitarized zones in republic of korea. | the trend of military patients becoming infected with vivax malaria reemerged in the republic of korea (rok) in 1993. the common explanation has been that infective anopheles mosquitoes from the democratic people's republic of korea have invaded republic of korea's demilitarized zone (dmz). the aim of this study was to verify the relationship between meteorological factors and the number of malaria patients in the military in this region. | 2016 | 26955803 |
| comparative evaluation of the efficiency of the bg-sentinel trap, cdc light trap and mosquito-oviposition trap for the surveillance of vector mosquitoes. | the surveillance of vector mosquitoes is important for the control of mosquito-borne diseases. to identify a suitable surveillance tool for the adult dengue vector aedes albopictus, the efficacy of the bg-sentinel trap, cdc light trap and mosquito-oviposition trap (mot) on the capture of vector mosquitoes were comparatively evaluated in this study. | 2016 | 27519419 |
| structure, spatial and temporal distribution of the culex pipiens complex in shanghai, china. | culex pipiens molestus was first reported in shanghai in 2010. the population structures and seasonal distributions of culex pipiens subspecies c. p. molestus, culex pipiens pallens, and culex pipiens quinquefasciatus are not well known. | 2016 | 27869687 |
| impact of interventions on malaria in internally displaced persons along the china-myanmar border: 2011-2014. | internally displaced persons (idp) represent vulnerable populations whose public health conditions merit special attention. in the china-myanmar border area, human movement and resettlements of idp can influence malaria transmission. comparison of disease incidence and vector densities between idp camps and surrounding local villages allows for better understanding of current epidemiology and to evaluate the effectiveness of interventions in the region. | 2016 | 27628040 |
| effects of microclimate condition changes due to land use and land cover changes on the survivorship of malaria vectors in china-myanmar border region. | in the past decade, developing countries have been experiencing rapid land use and land cover changes, including deforestation and cultivation of previously forested land. however, little is known about the impact of deforestation and land-use changes on the life history of malaria vectors and their effects on malaria transmission. this study examined the effects of deforestation and crop cultivation on the adult survivorship of major malaria mosquitoes, anopheles sinensis and an. minimus in the ... | 2016 | 27171475 |
| distribution of malaria vectors and incidence of vivax malaria at korean army installations near the demilitarized zone, republic of korea. | as a result of the reintroduction of malaria in the republic of korea (rok) in 1993 and the threat to military and civilian populations, the korea military national defense (mnd) increased emphasis on vector control in 2012 at rok army (roka) installations located near the dmz, while decreasing chemoprophylaxis, fearing potential drug resistance. mosquito surveillance demonstrated a need for continuous monitoring of disease patterns among roka soldiers and vector malaria infection rates to ensur ... | 2016 | 27150110 |
| salivary gland proteome during adult development and after blood feeding of female anopheles dissidens mosquitoes (diptera: culicidae). | understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. anopheles dissidens (formerly anopheles barbirostris species a1) is a potential vector of plasmodium vivax in thailand. in this study, analyses of the proteomic profiles of female an. dissidens salivary glands during adult development and after blood feeding were carried out us ... | 2016 | 27669021 |
| diversity and frequency of kdr mutations within anopheles sinensis populations from guangxi, china. | anopheles sinensis is a major vector of malaria in china and its control is under great threat as the development of insecticide resistance. voltage-gated sodium channel (vgsc) is the target of several classes of insecticides. genetic mutations of vgsc have been documented to confer knockdown resistance (kdr) to dichlorodiphenyltrichloroethane (ddt) and pyrethroids in mosquitoes. to control this vector efficiently, it is important to know the resistance-associated genetic mutations, their distri ... | 2016 | 27527509 |
| extensive genetic diversity of rickettsiales bacteria in multiple mosquito species. | rickettsiales are important zoonotic pathogens, causing severe disease in humans globally. although mosquitoes are an important vector for diverse pathogens, with the exception of members of the genus wolbachia little is known about their role in the transmission of rickettsiales. herein, rickettsiales were identified by pcr in five species of mosquitoes (anopheles sinensis, armigeres subalbatus, aedes albopictus, culex quinquefasciatus and cu. tritaeniorhynchus) collected from three chinese pro ... | 2016 | 27934910 |
| proteomic profile of the bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole. | benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against sclerotinia sclerotiorum, ditylenchus destructor and bradysia odoriphaga. however, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. here, we employed itraq-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of b. odoriphaga. in response to benzothiazole, 92 of ... | 2016 | 27883048 |
| tyrosine hydroxylase is crucial for maintaining pupal tanning and immunity in anopheles sinensis. | tyrosine hydroxylase (th), the initial enzyme in the melanin pathway, catalyzes tyrosine conversion into dopa. although expression and regulation of th have been shown to affect cuticle pigmentation in insects, no direct functional studies to date have focused on the specific physiological processes involving the enzyme during mosquito development. in the current study, silencing of asth during the time period of continuous high expression in anopheles sinensis pupae led to significant impairmen ... | 2016 | 27416870 |
| rickettsia in mosquitoes, yangzhou, china. | 2016 | 27729642 | |
| challenges with using names to link digital biodiversity information. | 2016 | 27346955 | |
| annotation of the asian citrus psyllid genome reveals a reduced innate immune system. | citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as huanglongbing. the citrus greening bacteria, candidatus liberibacter asiaticus (clas), is a persistent propagative pathogen transmitted by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). hemipterans characterized to date lack a number of insect immune genes, including those associated with the imd pathway targeting gram-negative bacteria. the d. citri draft ... | 2016 | 27965582 |
| viral metagenomics on blood-feeding arthropods as a tool for human disease surveillance. | surveillance and monitoring of viral pathogens circulating in humans and wildlife, together with the identification of emerging infectious diseases (eids), are critical for the prediction of future disease outbreaks and epidemics at an early stage. it is advisable to sample a broad range of vertebrates and invertebrates at different temporospatial levels on a regular basis to detect possible candidate viruses at their natural source. however, virus surveillance systems can be expensive, costly i ... | 2016 | 27775568 |
| comparative studies on the stenogamous and eurygamous behavior of eight anopheles species of the hyrcanus group (diptera: culicidae) in thailand. | establishment of laboratory colony is essential for mosquito-borne-disease research. mating behavior of stenogamous anopheles peditaeniatus and seven eurygamous species (anopheles argyropus, anopheles crawfordi, anopheles nigerrimus, anopheles nitidus, anopheles paraliae (=an. lesteri), anopheles pursati and anopheles sinensis), were investigated and compared in this study. the self-mating success of adult mosquitoes in different size cages at two density resting surface (drs) values, 3.6 and 7. ... | 2016 | 27023618 |
| genome-wide identification and characterization of odorant-binding protein (obp) genes in the malaria vector anopheles sinensis (diptera: culicidae). | anopheles sinensis is a major malaria vector. insect odorant-binding proteins (obps) may function in the reception of odorants in the olfactory system. the classification and characterization of the an. sinensis obp genes have not been systematically studied. in this study, 64 putative obp genes were identified at the whole-genome level of an. sinensis based on the comparison between obp conserved motifs, pbp_gobp, and phylogenetic analysis with an. gambiae obps. the characterization of an. sine ... | 2016 | 26970073 |
| sialotranscriptome sequencing and analysis of anopheles sinensis and comparison with psorophora albipes sialotranscriptome (diptera: culicidae). | most of adult female mosquitoes secrete saliva to facilitate blood sucking, digestion and nutrition, and mosquito-borne disease prevention. the knowledge of classification and characteristics of sialotranscriptome genes are still quite limited. anopheles sinensis is a major malaria vector in china and southeast asian countries. in this study, the an. sinensis sialotranscriptome was sequenced using illumina sequencing technique with a total of 10 907 unigenes to be obtained and annotated in biolo ... | 2016 | 27996203 |
| vector competence of anopheles kleini and anopheles sinensis (diptera: culicidae) from the republic of korea to vivax malaria-infected blood from patients from thailand. | in total, 1,300 each of anopheles kleini rueda and anopheles sinensis wiedemann sensu stricto (s.s.) females (colonized from the republic of korea) and anopheles dirus peyton & harrison (thai strain) were allowed to feed on blood from thai malaria patients naturally infected with plasmodium vivax the overall oocyst infection rates for an. dirus, an. kleini, and an. sinensis s.s. were 77.4, 46.1, and 45.9%, respectively. the mean number of oocysts was significantly higher for an. dirus (82.7) com ... | 2016 | 27493248 |
| a neural network prediction of environmental determinants of anopheles sinensis knockdown resistance mutation to pyrethroids in china. | selection pressure caused by long-term intensive use of insecticides is the key driving force in resistance development. additional parameters such as environmental conditions may affect both the mosquito response to insecticides and the selection of resistance mechanisms. in this context, we analyzed the environmental determinants of kdr prevalence in anopheles sinensis across china. we collected kdr frequency from 48 sites across central and southern china, together with key environmental fact ... | 2016 | 27860007 |
| larvicidal activity of magnolia denudata seed hydrodistillate constituents and related compounds and liquid formulations towards two susceptible and two wild mosquito species. | anopheles sinensis, aedes aegypti and aedes albopictus and culex pipiens pallens mosquitoes transmit malaria, dengue fever and west nile virus diseases respectively. this study was conducted to determine the toxicity of 17 constituents from magnolia denudata seed hydrodistillate (md-shd) and four experimental md-shd liquid formulations (10-50 mg l(-1) liquid) to third-instar larvae from insecticide-susceptible cx. p. pallens and ae. aegypti as well as wild ae. albopictus and an. sinensis. | 2016 | 26085316 |
| [genetic polymophism and evolution of srpn14 gene in anopheles sinensis (diptera : culicidae)]. | to identify and locate the serine protease inhibitor 14 (srpn14) gene of anopheles sinensis, and analyze its genetic polymorphism among populations as well as the selective pressure during evolution. | 2015 | 26672210 |
| isolation of japanese encephalitis virus and a novel insect-specific flavivirus from mosquitoes collected in a cowshed in japan. | cattle do not generally appear to develop severe viremia when infected with japanese encephalitis virus (jev), and they can be infected without showing clinical signs. however, two cattle in japan recently died from jev infection. in this study, we investigated the presence of different species of mosquitoes and flavivirus in a cowshed in the southwest region of japan. in this cowshed, the two most common species of mosquitoes collected were culex tritaeniorhynchus (including culex pseudovishnui ... | 2015 | 26085283 |
| the relationship between wing length, blood meal volume, and fecundity for seven colonies of anopheles species housed at the armed forces research institute of medical sciences, bangkok, thailand. | established colonies of anopheles campestris, anopheles cracens, anopheles dirus, anopheles kleini, anopheles minimus, anopheles sawadwongporni, and anopheles sinensis are maintained at the armed forces research institute of medical sciences (afrims). females were provided blood meals on human blood containing citrate as an anticoagulant using an artificial membrane feeder. the mean wing length, used as an estimate of body size, for each species was compared to blood-feeding duration (time), blo ... | 2015 | 26433074 |
| [analysis of population genetic structure of anopheles sinensis based on mitochondrial dna cytochrome oxidase subunit i gene fragment]. | to study the population genetic variation, genetic diversity and phylogenesis of anopheles sinensis in china. | 2015 | 25736119 |
| distribution and phylogenetic analysis of culex flavivirus in mosquitoes in china. | culex flavivirus (cxfv) is an insect-specific virus of the genus flavivirus. cxfv strains have been isolated from cx. pipiens, cx. quinquefasciatus, and other cx. species in asia, africa, north america, central america and south america. cxfv was isolated for the first time in china in 2006. as this is a novel flavivirus, we explored the distribution and genetic characteristics of culex flavivirus in china. a total of 46,649 mosquitoes were collected in seven provinces between 2004 and 2012 and ... | 2015 | 26118548 |
| population genetic structure of the malaria vector anopheles sinensis (diptera: culicidae) sensu stricto and evidence for possible introgression in the republic of korea. | anopheles sinensis wiedemann sensu stricto (s.s.) is a dominant mosquito and considered a secondary malaria vector in the republic of korea (rok). despite the potential significance for malaria control, population genetics studies have been conducted using only mitochondrial dna (mtdna), and studies of the genetics of hybridization have never been attempted. in this study, 346 specimens from 23 localities were subject to experiments. among them, 305 an. sinensis s.s. specimens from 20 localities ... | 2015 | 26336253 |
| unprecedented genomic diversity of rna viruses in arthropods reveals the ancestry of negative-sense rna viruses. | although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. through rna sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense rna viruses, a number of which are also present as endogenous genomic copies. with this greatly enriched diversity we revealed that arthropods contain ... | 2015 | 25633976 |
| mosquito rasputin interacts with chikungunya virus nsp3 and determines the infection rate in aedes albopictus. | chikungunya virus (chikv) is an arthritogenic alphavirus (family togaviridae), transmitted by aedes species mosquitoes. chikv re-emerged in 2004 with multiple outbreaks worldwide and recently reached the americas where it has infected over a million individuals in a rapidly expanding epidemic. while alphavirus replication is well understood in general, the specific function (s) of non-structural protein nsp3 remain elusive. chikv nsp3 modulates the mammalian stress response by preventing stress ... | 2015 | 26384002 |
| insect-specific flaviviruses: a systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization. | there has been a dramatic increase in the number of insect-specific flaviviruses (isfs) discovered in the last decade. historically, these viruses have generated limited interest due to their inability to infect vertebrate cells. this viewpoint has changed in recent years because some isfs have been shown to enhance or suppress the replication of medically important flaviviruses in co-infected mosquito cells. additionally, comparative studies between isfs and medically important flaviviruses can ... | 2015 | 25866904 |
| risky behaviors: effects of toxorhynchites splendens (diptera: culicidae) predator on the behavior of three mosquito species. | viable biocontrol agents for mosquito control are quite rare, therefore improving the efficacy of existing biological agents is an important study. we need to have a better understanding of the predation-risk behavioral responses toward prey. this research examined prey choices by toxorhynchites splendens by monitoring the behavioral responses of aedes aegypti, aedes albopictus, and anopheles sinensis larvae when exposed to the predator. the results show that tx. splendens prefers to consume ae. ... | 2015 | 26386041 |
| a genome-scale investigation of incongruence in culicidae mosquitoes. | comparison of individual gene trees in several recent phylogenomic studies from diverse lineages has revealed a surprising amount of topological conflict or incongruence, but we still know relatively little about its distribution across the tree of life. to further our understanding of incongruence, the factors that contribute to it and how it can be ameliorated, we examined its distribution in a clade of 20 culicidae mosquito species through the reconstruction and analysis of the phylogenetic h ... | 2015 | 26608059 |
| the cpcfc cuticular protein family: anatomical and cuticular locations in anopheles gambiae and distribution throughout pancrustacea. | arthropod cuticles have, in addition to chitin, many structural proteins belonging to diverse families. information is sparse about how these different cuticular proteins contribute to the cuticle. most cuticular proteins lack cysteine with the exception of two families (cpap1 and cpap3), recently described, and the one other that we now report on that has a motif of 16 amino acids first identified in a protein, bc-ncp1, from the cuticle of nymphs of the cockroach, blaberus craniifer (jensen et ... | 2015 | 26164413 |
| rotational symmetry of two pyrethroid receptor sites in the mosquito sodium channel. | voltage-gated sodium channels are the primary target of pyrethroid insecticides. although it is well known that specific mutations in insect sodium channels confer knockdown resistance (kdr) to pyrethroids, the atomic mechanisms of pyrethroid-sodium channel interactions are not clearly understood. previously, computer modeling and mutational analysis predicted two pyrethroid receptors, pyrethroid receptor site 1 (pyr1) (initial) and pyrethroid receptor site 2 (pyr2), located in the domain interf ... | 2015 | 25972447 |
| knockdown resistance (kdr) mutations in indian anopheles culicifacies populations. | anopheles culicifacies s.l. is one of the primary vectors of malaria in india responsible for the highest number of malaria cases. this vector is resistant to ddt in most parts of the country with indication of emerging resistance to pyrethroids. since knockdown resistance (kdr) is known to confer cross-resistance between ddt and pyrethroids owing to a common target site of action, knowledge of prevalence of knockdown resistance (kdr) alleles is important from insecticide resistance management p ... | 2015 | 26082160 |
| population dynamics and community structure of anopheles mosquitoes along the china-myanmar border. | understanding the ecology of malaria vectors such as species composition and population dynamics is essential for developing cost-effective strategies to control mosquito vector populations. | 2015 | 26338527 |
| insecticide resistance in areas under investigation by the international centers of excellence for malaria research: a challenge for malaria control and elimination. | scale-up of the main vector control interventions, residual insecticides sprayed on walls or structures and/or impregnated in bed nets, together with prompt diagnosis and effective treatment, have led to a global reduction in malaria transmission. however, resistance in vectors to almost all classes of insecticides, particularly to the synthetic pyrethroids, is posing a challenge to the recent trend of declining malaria. ten international centers of excellence for malaria research (icemr) locate ... | 2015 | 26259947 |
| entomological monitoring and evaluation: diverse transmission settings of icemr projects will require local and regional malaria elimination strategies. | the unprecedented global efforts for malaria elimination in the past decade have resulted in altered vectorial systems, vector behaviors, and bionomics. these changes combined with increasingly evident heterogeneities in malaria transmission require innovative vector control strategies in addition to the established practices of long-lasting insecticidal nets and indoor residual spraying. integrated vector management will require focal and tailored vector control to achieve malaria elimination. ... | 2015 | 26259942 |
| diversification of the genus anopheles and a neotropical clade from the late cretaceous. | the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ... | 2015 | 26244561 |
| a metagenomic survey of viral abundance and diversity in mosquitoes from hubei province. | mosquitoes as one of the most common but important vectors have the potential to transmit or acquire a lot of viruses through biting, however viral flora in mosquitoes and its impact on mosquito-borne disease transmission has not been well investigated and evaluated. in this study, the metagenomic techniquehas been successfully employed in analyzing the abundance and diversity of viral community in three mosquito samples from hubei, china. among 92,304 reads produced through a run with 454 gs fl ... | 2015 | 26030271 |
| genomic analysis of detoxification supergene families in the mosquito anopheles sinensis. | anopheles sinensis is an important malaria vector in china and other southeast asian countries, and the emergence of insecticide resistance in this mosquito poses a serious threat to the efficacy of malaria control programs. the recently published an. sinensis genome and transcriptome provide an opportunity to understand the molecular mechanisms of insecticide resistance. analysis of the an. sinensis genome revealed 174 detoxification genes, including 93 cytochrome p450s (p450s), 31 glutathione- ... | 2015 | 26588704 |
| risk assessment of malaria prevalence in ludian, yongshan, and jinggu counties, yunnan province, after 2014 earthquake disaster. | the objective of this study was to investigate malaria prevalence after the 2014 earthquakes in ludian, yongshan, and jinggu counties, yunnan province, china. we collected and analyzed epidemiological data and made a risk assessment of transmission probability. from january 2005 to july 2015, 87 malaria cases were reported in the three counties, most of which (81.6%) occurred between 2005 and 2009, with five cases reported in jinggu county between january 2014 and july 2015, of which one case wa ... | 2015 | 26711514 |
| distribution and frequency of g119s mutation in ace-1 gene within anopheles sinensis populations from guangxi, china. | malaria is one of the most serious vector-borne diseases in the world. vector control is an important measure for malaria prevention and elimination. however, this strategy is under threat as disease vectors are developing resistance to insecticides. therefore, it is important to monitor mechanisms responsible for insecticide resistance. in this study, the presence of g119s mutation in the acetyl cholinesterase-encoding gene (ace-1) was investigated in nine anopheles sinensis populations sampled ... | 2015 | 26608572 |
| the plasmodium vivax in china: decreased in local cases but increased imported cases from southeast asia and africa. | currently the local p. vivax was sharply decreased while the imported vivax malaria increased in china. despite southeast asia was still the main import source of vivax malaria, the trend of africa become serious, especially for west and central africa. herein we have clarified the trend of p. vivax in china from 2004-2012, and made some analysis for the differences of imported vivax back from different regions. there are significantly different of p. vivax between southeast asia and africa, als ... | 2015 | 25739365 |
| spatial-temporal variation and primary ecological drivers of anopheles sinensis human biting rates in malaria epidemic-prone regions of china. | robust malaria vector surveillance is essential for optimally selecting and targeting vector control measures. sixty-two vector surveillance sites were established between 2005 and 2008 by the national malaria surveillance program in china to measure anopheles sinensis human biting rates. using these data to determine the primary ecological drivers of malaria vector human biting rates in malaria epidemic-prone regions of china will allow better targeting of vector control resources in space and ... | 2015 | 25611483 |
| malaria in zhejiang province, china, from 2005 to 2014. | to summarize the changing epidemiological characteristics of malaria in zhejiang province, china, we collected data on malaria from the chinese notifiable disease reporting system (ndrs) and analyzed them. a total of 2,738 malaria cases were identified in zhejiang province from 2005 to 2014, of which 2,018 were male and 720 were female. notably, only 7% of malaria cases were indigenous and the other cases were all imported. the number of malaria cases increased from 2005 to 2007, peaked in 2007, ... | 2015 | 26078321 |
| a call for benchmarking transposable element annotation methods. | dna derived from transposable elements (tes) constitutes large parts of the genomes of complex eukaryotes, with major impacts not only on genomic research but also on how organisms evolve and function. although a variety of methods and tools have been developed to detect and annotate tes, there are as yet no standard benchmarks-that is, no standard way to measure or compare their accuracy. this lack of accuracy assessment calls into question conclusions from a wide range of research that depends ... | 2015 | 26244060 |
| development of insecticide resistance in malaria vector anopheles sinensis populations from shandong province in china. | anopheles sinensis is a major vector of malaria and among the dominant species in shandong province of china. insecticide resistance is an important threat to vector-borne disease control. however, there are only few reports about insecticide resistance of an. sinensis populations from shandong province. | 2015 | 25880316 |