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effects of an inducible aiia gene on disease resistance in eucalyptus urophylla × eucalyptus grandis.n-acyl-homoserine lactones (ahls) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. at threshold concentrations, ahls can activate the expression of pathogenic genes and induce diseases. therefore, reducing ahl concentrations is a key point of disease control in plants. ahl-lactonase, which is expressed by aiia, is widespread in bacillus sp and can hydrolyze ahls. in the present study, we cloned aiia from bacillus subtilis ...201626905275
dual rna-sequencing of eucalyptus nitens during phytophthora cinnamomi challenge reveals pathogen and host factors influencing compatibility.damage caused by phytophthora cinnamomi rands remains an important concern on forest tree species. the pathogen causes root and collar rot, stem cankers, and dieback of various economically important eucalyptus spp. in south africa, susceptible cold tolerant eucalyptus plantations have been affected by various phytophthora spp. with p. cinnamomi considered one of the most virulent. the molecular basis of this compatible interaction is poorly understood. in this study, susceptible eucalyptus nite ...201626973660
diversity and distribution of the endophytic bacterial community at different stages of eucalyptus growth.the relationships between plants and endophytic bacteria significantly contribute to plant health and yield. however, the microbial diversity in leaves of eucalyptus spp. is still poorly characterized. here, we investigated the endophytic diversity in leaves of hybrid eucalyptus grandis x e. urophylla (eucalyptus "urograndis") by using culture-independent and culture-dependent approaches, to better understand their ecology in leaves at different stages of eucalyptus development, including bacter ...201627010209
a novel epiphytic cyanobacterial species from the genus brasilonema causing damage to eucalyptus leaves(1).a cyanobacterial mat colonizing the leaves of eucalyptus grandis was determined to be responsible for serious damage affecting the growth and development of whole plants under the clonal hybrid nursery conditions. the dominant cyanobacterial species was isolated in bg-11 medium lacking a source of combined nitrogen and identified by cell morphology characters and molecular phylogenetic analysis (16s rrna gene and cpcba-igs sequences). the isolated strain represents a novel species of the genus b ...200827041729
in situ enzyme aided adsorption of soluble xylan biopolymers onto cellulosic material.the functional properties of cellulose fibers can be modified by adsorption of xylan biopolymers. the adsorption is improved when the degree of biopolymers substitution with arabinose and 4-o-methyl-glucuronic acid (meglca) side groups, is reduced. α-l-arabinofuranosidase (abfb) and α-d-glucuronidase (agua) enzymes were applied for side group removal, to increase adsorption of xylan from sugarcane (saccharum officinarum l) bagasse (bh), bamboo (bambusa balcooa) (bm), pinus patula (pp) and eucaly ...201627083357
transcriptional profiles of hybrid eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition.eucalyptus species constitutes the most widely planted hardwood trees in temperate and subtropical regions. in this study, we compared the transcript levels of genes involved in lignocellulose formation such as cellulose, hemicellulose and lignin biosynthesis in two selected 3-year old hybrid eucalyptus (eucalyptus urophylla × eucalyptus grandis) genotypes (am063 and am380) that have different lignin content. am063 and am380 had 20.2 and 35.5% of klason lignin content and 59.0 and 48.2%, α-cellu ...201627148283
antifungal activity of eucalyptus oil against rice blast fungi and the possible mechanism of gene expression pattern.eucalyptus oil possesses a wide spectrum of biological activity, including anti-microbial, fungicidal, herbicidal, acaricidal and nematicidal properties. we studied anti-fungal activities of the leaf oil extracted from eucalyptus. grandis × e. urophylla. eleven plant pathogenic fungi were tested based on the mycelium growth rates with negative control. the results showed that eucalyptus oil has broad-spectrum inhibitory effects toward these fungi. remarkable morphological and structural alterati ...201627187335
effects of autohydrolysis of eucalyptus urograndis and eucalyptus grandis on influence of chemical components and crystallinity index.samples of eucalyptus urograndis and eucalyptus grandis sawdust were autohydrolyzed in aqueous conditions to reach temperatures in the range 110-190°c and reaction times of 0-150min in a minireactor. in each minireactor were used a liquor:wood ratio (10:1 l:kg dry wood), in order to assess the effects of the autohydrolysis severity and the crystalline properties of cellulose. the content of extractives, lignin, holocellulose, cellulose, hemicelluloses and crystallinity index obtained from the so ...201627187566
intraspecific competition between ectomycorrhizal pisolithus microcarpus isolates impacts plant and fungal performance under elevated co2 and temperature.root systems are simultaneously colonized by multiple individuals of mycorrhizal fungi. intraspecific competitive interactions between fungal isolates are likely to affect both fungal and plant performance and be influenced by abiotic factors. here, we assessed the impact of intraspecific competition between three pisolithus microcarpus isolates on the establishment of, and benefit derived from, symbioses with eucalyptus grandis seedlings. we investigated the outcomes of competition under ambien ...201627222224
constancy, distribution, and frequency of lepidoptera defoliators of eucalyptus grandis and eucalyptus urophylla (myrtaceae) in four brazilian regions.the growth of the brazilian forest sector with monocultures favors the adaptation of arthropoda pests. the lepidoptera order includes major pests of eucalyptus spp. (myrtaceae). the aim of this work is to study the population constancy, distribution, and frequency of lepidoptera primary pests of eucalyptus spp. lepidoptera pests in eucalyptus spp. plantations were collected in três marias and guanhães (state of minas gerais), niquelândia (state of goiás), and monte dourado (state of pará), brazi ...201627255768
a snare-like superfamily protein sbslsp from the halophyte salicornia brachiata confers salt and drought tolerance by maintaining membrane stability, k(+)/na(+) ratio, and antioxidant machinery.about 1000 salt-responsive ests were identified from an extreme halophyte salicornia brachiata. among these, a novel salt-inducible gene sbslsp (salicornia brachiata snare-like superfamily protein), showed up-regulation upon salinity and dehydration stress. the presence of cis-regulatory motifs related to abiotic stress in the putative promoter region supports our finding that sbslsp gene is inducible by abiotic stress. the sbslsp protein showed a high sequence identity to hypothetical/uncharact ...201627313584
supercritical water gasification of eucalyptus grandis and related pyrolysis char: effect of feedstock composition.eucalyptus grandis (e. grandis) wood and char products derived from pyrolysis of e. grandis wood, were gasified in supercritical water at 450°c - with and without the use of a homogeneous (k2co3) and heterogeneous (ni/al2o3-sio2) catalyst. gas yields and gasification efficiencies were measured experimentally and compared to calculated thermodynamic equilibrium values, specifically considering the effects of the o/c ratio and volatile matter content of the feed material. thermodynamically, feed m ...201627343456
integrated analysis of gene expression from carbon metabolism, proteome and metabolome, reveals altered primary metabolism in eucalyptus grandis bark, in response to seasonal variation.seasonal variation is presumed to play an important role in the regulation of tree growth, especially for eucalyptus grandis, a fast-growing tree. this variation may induce changes in the whole tree at transcriptional, protein and metabolite levels. bark represents an important group of tissues that protect trees from desiccation and pathogen attack, and it has been identified as potential feedstock for lignocellulosic derived biofuels. despite the growing interest, little is known about the mol ...201627364638
seasonal variation of carbon metabolism in the cambial zone of eucalyptus grandis.eucalyptus species are the most widely hardwood planted in the world. it is one of the successful examples of commercial forestry plantation in brazil and other tropical and subtropical countries. the tree is valued for its rapid growth, adaptability and wood quality. wood formation is the result of cumulative annual activity of the vascular cambium. this cambial activity is generally related to the alternation of cold and warm, and/or dry and rainy seasons. efforts have focused on analysis of c ...201627446160
allometric models and aboveground biomass stocks of a west african sudan savannah watershed in benin.the estimation of forest biomass changes due to land-use change is of significant importance for estimates of the global carbon budget. the accuracy of biomass density maps depends on the availability of reliable allometric models used in combination with data derived from satellites images and forest inventory data. to reduce the uncertainty in estimates of carbon emissions resulting from deforestation and forest degradation, better information on allometric equations and the spatial distributi ...201627594897
genome scans for divergent selection in natural populations of the widespread hardwood species eucalyptus grandis (myrtaceae) using microsatellites.identification of loci or genes under natural selection is important for both understanding the genetic basis of local adaptation and practical applications, and genome scans provide a powerful means for such identification purposes. in this study, genome-wide simple sequence repeats markers (ssrs) were used to scan for molecular footprints of divergent selection in eucalyptus grandis, a hardwood species occurring widely in costal areas from 32° s to 16° s in australia. high population diversity ...201627748400
plant species differ in early seedling growth and tissue nutrient responses to arbuscular and ectomycorrhizal fungi.experiments with plant species that can host both arbuscular mycorrhizal fungi (amf) and ectomycorrhizal fungi (emf) are important to separating the roles of fungal type and plant species and understanding the influence of the types of symbioses on plant growth and nutrient acquisition. we examined the effects of mycorrhizal fungal type on the growth and tissue nutrient content of two tree species (eucalyptus grandis and quercus costaricensis) grown under four nutrient treatments (combinations o ...201727838856
special trends in cbf and dreb2 groups in eucalyptus gunnii vs eucalyptus grandis suggest that cbf are master players in the trade-off between growth and stress resistance.annotation of the eucalyptus grandis genome showed a large amplification of the dehydration-responsive element binding 1/c-repeat binding factor (dreb1/cbf) group without recent dreb2 gene duplication compared with other plant species. the present annotation of the cbf and dreb2 genes from a draft of the eucalyptus gunnii genome sequence reveals at least one additional cbf copy in the e. gunnii genome compared with e. grandis, suggesting that this group is still evolving, unlike the dreb2 group. ...201727861954
genome-wide analysis of the tpx2 family proteins in eucalyptus grandis.the xklp2 (tpx2) proteins belong to the microtubule-associated (map) family of proteins. all members of the family contain the conserved tpx2 motif, which can interact with microtubules, regulate microtubule dynamics or assist with different microtubule functions, for example, maintenance of cell morphology or regulation of cell growth and development. however, the role of members of the tpx family have not been studied in the model tree species eucalyptus to date. here, we report the identifica ...201627881090
the eucalyptus tonoplast intrinsic protein (tip) gene subfamily: genomic organization, structural features, and expression profiles.plant aquaporins are water channels implicated in various physiological processes, including growth, development and adaptation to stress. in this study, the tonoplast intrinsic protein (tip) gene subfamily of eucalyptus, an economically important woody species, was investigated and characterized. a genome-wide survey of the eucalyptus grandis genome revealed the presence of eleven putative tip genes (referred as egtip), which were individually assigned by phylogeny to each of the classical tip1 ...201627965702
localization and transcriptional responses of chrysoporthe austroafricana in eucalyptus grandis identify putative pathogenicity factors.chrysoporthe austroafricana is a fungal pathogen that causes the development of stem cankers on susceptible eucalyptus grandis trees. clones of e. grandis that are partially resistant and highly susceptible have been identified based on the extent of lesion formation on the stem upon inoculation with c. austroafricana. these interactions have been used as a model pathosystem to enhance our understanding of interactions between pathogenic fungi and woody hosts, which may be different to herbaceou ...201628008326
nosema ceranae winter control: study of the effectiveness of different fumagillin treatments and consequences on the strength of honey bee (hymenoptera: apidae) colonies.in uruguay, colonies of honey bees moving to eucalyptus grandis plantation in autumn habitually become infected with the microsporidian nosema ceranae , a parasite that attacks the digestive system of bees. beekeepers attributed to n. ceranae depopulation of the colonies that often occurs at the end of the blooming period, and many use the antibiotic fumagillin to reduce the level of infection. the aim of this study was to compare the effectiveness of four different fumagillin treatments and det ...201728025388
allelopathic effects of volatile organic compounds from eucalyptus grandis rhizosphere soil on eisenia fetida assessed using avoidance bioassays, enzyme activity, and comet assays.allelopathy has been identified as an underlying mechanism of detrimental environmental impacts within commercial plantations. eucalyptus spp. are known to generate huge amounts of volatile organic compounds (vocs) that can function as phytotoxins and thus inhibit other plants. in the present study, biochemical markers, including activities of acetylcholinesterase (ache) and oxidative stress enzymes, such as superoxide dismutase (sod) and glutathione s-transferase (gst), were assayed to assess c ...201728113065
insights into temperature modulation of the eucalyptus globulus and eucalyptus grandis antioxidant and lignification subproteomes.eucalyptus grandis and eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas, as e. grandis is mostly cultivated in tropical regions while e. globulus is preferred in temperate areas. as temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes in the antioxidant system and the lignification metabolism in plantlets grown at different temperatures. our strategy allowed t ...201728190676
fungal planet description sheets: 469-557.novel species of fungi described in this study include those from various countries as follows: australia: apiognomonia lasiopetali on lasiopetalum sp., blastacervulus eucalyptorum on eucalyptus adesmophloia, bullanockia australis (incl. bullanockia gen. nov.) on kingia australis, caliciopsis eucalypti on eucalyptus marginata, celerioriella petrophiles on petrophile teretifolia, coleophoma xanthosiae on xanthosia rotundifolia, coniothyrium hakeae on hakea sp., diatrypella banksiae on banksia for ...201628232766
genome-wide analysis of egeve_1, a transcriptionally active endogenous viral element associated to small rnas in eucalyptus genomes.endogenous viral elements (eves) are the result of heritable horizontal gene transfer from viruses to hosts. in the last years, several eve integration events were reported in plants by the exponential availability of sequenced genomes. eucalyptus grandis is a forest tree species with a sequenced genome that is poorly studied in terms of evolution and mobile genetic elements composition. here we report the characterization of e. grandis endogenous viral element 1 (egeve_1), a transcriptionally a ...201728235127
fuzzy logic applied to prospecting for areas for installation of wood panel industries.prospecting for suitable areas for forestry operations, where the objective is a reduction in production and transportation costs, as well as the maximization of profits and available resources, constitutes an optimization problem. however, fuzzy logic is an alternative method for solving this problem. in the context of prospecting for suitable areas for the installation of wood panel industries, we propose applying fuzzy logic analysis for simulating the planting of different species and eucaly ...201728237845
root morphogenic pathways in eucalyptus grandis are modified by the activity of protein arginine methyltransferases.methylation of proteins at arginine residues, catalysed by members of the protein arginine methyltransferase (prmt) family, is crucial for the regulation of gene transcription and for protein function in eukaryotic organisms. inhibition of the activity of prmts in annual model plants has demonstrated wide-ranging involvement of prmts in key plant developmental processes, however, prmts have not been characterised or studied in long-lived tree species.201728279165
transcript profiling of a novel plant meristem, the monocot cambium.while monocots lack the ability to produce a vascular cambium or woody growth, some monocot lineages evolved a novel lateral meristem, the monocot cambium, which supports secondary radial growth of stems. in contrast to the vascular cambium found in woody angiosperm and gymnosperm species, the monocot cambium produces secondary vascular bundles, which have an amphivasal organization of tracheids encircling a central strand of phloem. currently there is no information concerning the molecular gen ...201728304126
model simulations of spatial distributions and daily totals of photosynthesis in eucalyptus grandis canopies.a simulation model for radiation absorption and photosynthesis was used to test the hypothesis that observed nonuniform distributions of nitrogen concentrations in young eucalyptus grandis trees result in greater amounts of daily assimilation than in hypothetical trees with uniform n distributions. simulations were performed for trees aged 6, 9, 12 and 16 months which had been grown in plantations under a factorial combination of two levels of fertilization and irrigation. observed leaf n distri ...199128312618
spatial distributions of foliar nitrogen and phosphorus in crowns of eucalyptus grandis.eucalyptus grandis trees were grown in plantations with and without added fertiliser to examine the effects of plant nutrition on photosynthesis and growth. leaves were sampled from known locations within canopies of selected trees and leaf n and p concentrations were measured. contour maps of n and p distributions were then produced for crowns of trees aged between 6 and 16 months. gas exchange measurements on sample leaves were used to estimate parameters of a model of c3 photosynthesis as a f ...199128312619
identification of the eucalyptus grandis chitinase gene family and expression characterization under different biotic stress challenges.eucalyptus grandis (w. hill ex maiden) is an australian myrtaceae tree grown for timber in many parts of the world and for which the annotated genome sequence is available. known to be susceptible to a number of pests and diseases, e. grandis is a useful study organism for investigating defense responses in woody plants. chitinases are widespread in plants and cleave glycosidic bonds of chitin, the major structural component of fungal cell walls and arthropod exoskeletons. they are encoded by an ...201728338992
evidence for salicylic acid signalling and histological changes in the defence response of eucalyptus grandis to chrysoporthe austroafricana.eucalyptus species are cultivated for forestry and are of economic importance. the fungal stem canker pathogen chrysoporthe austroafricana causes disease of varying severity on e. grandis. the eucalyptus grandis-chrysoporthe austroafricana interaction has been established as a model system for studying eucalyptus antifungal defence. previous studies revealed that the phytohormone salicylic acid (sa) affects the levels of resistance in highly susceptible (zg14) and moderately resistant (tag5) clo ...201728349984
cellulose nanofibers from softwood, hardwood, and tunicate: preparation-structure-film performance interrelation.this work reveals the structural variations of cellulose nanofibers (cnf) prepared from different cellulose sources, including softwood (picea abies), hardwood (eucalyptus grandis × e. urophylla), and tunicate (ciona intestinalis), using different preparation processes and their correlations to the formation and performance of the films prepared from the cnf. here, the cnf are prepared from wood chemical pulps and tunicate isolated cellulose by an identical homogenization treatment subsequent to ...201728350431
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