Publications

concerted action of two subunits of the functional dimer of shewanella oneidensis mr-1 uridine phosphorylase derived from a comparison of the c212s mutant and the wild-type enzyme.	uridine phosphorylase (up; ec 2.4.2.3), a key enzyme in the pyrimidine-salvage pathway, catalyzes the reversible phosphorolysis of uridine to uracil and ribose 1-phosphate. the structure of the c212s mutant of uridine phosphorylase from the facultatively aerobic gram-negative γ-proteobacterium shewanella oneidensis mr-1 (soup) was determined at 1.68 å resolution. a comparison of the structures of the mutant and the wild-type enzyme showed that one dimer in the mutant hexamer differs from all oth ...	2016	26894668
cyma and exogenous flavins improve extracellular electron transfer and couple it to cell growth in mtr-expressing escherichia coli.	introducing extracellular electron transfer pathways into heterologous organisms offers the opportunity to explore fundamental biogeochemical processes and to biologically alter redox states of exogenous metals for various applications. while expression of the mtrcab electron nanoconduit from shewanella oneidensis mr-1 permits extracellular electron transfer in escherichia coli, the low electron flux and absence of growth in these cells limits their practicality for such applications. here we in ...	2016	27000939
shewanella oneidensis fabb: a β-ketoacyl-acp synthase that works with c16:1-acp.	it is established that escherichia coli β-ketoacyl-acp synthase (kas) i (encoded by ecfabb) is the primary, if not exclusive, factor for elongation of the cis-3-decenoyl-acp (c10:1-acp) but not effective with c16:1- or longer-chain-acps. to test the extent to which these features apply to kas i proteins in other species, in this study, we examined the physiological role of fabb in shewanella oneidensis, an excellent model for researching type ii fatty acid synthetic (fas) system and its regulati ...	2016	27014246
real-time manganese phase dynamics during biological and abiotic manganese oxide reduction.	manganese oxides are often highly reactive and easily reduced, both abiotically, by a variety of inorganic chemical species, and biologically during anaerobic respiration by microbes. to evaluate the reaction mechanisms of these different reduction routes and their potential lasting products, we measured the sequence progression of microbial manganese(iv) oxide reduction mediated by chemical species (sulfide and ferrous iron) and the common metal-reducing microbe shewanella oneidensis mr-1 under ...	2016	27018915
adhesion of shewanella oneidensis mr-1 to goethite: a two-dimensional correlation spectroscopic study.	bacterial adhesion to mineral surfaces is an important but underappreciated process. to decipher the molecular level process and mechanism, the adhesion of shewanella oneidensis mr-1 cells to goethite was investigated using flow-cell attenuated total reflectance (atr) fourier transform infrared (ftir) spectroscopy coupled with two-dimensional correlation spectroscopy (2d-cos) analysis. the ftir results indicate that bacterial phosphate-moieties play an important role in the formation of mono- an ...	2016	27029565
trace heavy metal ions promoted extracellular electron transfer and power generation by shewanella in microbial fuel cells.	although microbial fuel cells (mfcs) is considered as one of the most promising technology for renewable energy harvesting, low power output still accounts one of the bottlenecks and limits its further development. in this work, it is found that cu(2+) (0.1μgl(-1)-0.1mgl(-1)) or cd(2+) (0.1μgl(-1)-1mgl(-1)) significantly improve the electricity generation in mfcs. the maximum power output achieved with trace level of cu(2+) (∼6nm) or cd(2+) (∼5nm) is 1.3 times and 1.6 times higher than that of t ...	2016	27038263
reduced expression of cytochrome oxidases largely explains camp inhibition of aerobic growth in shewanella oneidensis.	inhibition of bacterial growth under aerobic conditions by elevated levels of cyclic adenosine 3',5'-monophosphate (camp), first revealed more than 50 years ago, was attributed to accumulation of toxic methylglyoxal (mg). here, we report a crp-dependent mechanism rather than mg accumulation that accounts for the phenotype in shewanella oneidensis, an emerging research model for the bacterial physiology. we show that a similar phenotype can be obtained by removing cpda, a camp phosphodiesterase t ...	2016	27076065
shewanella oneidensis mr-1-induced fe(iii) reduction facilitates roxarsone transformation.	although microbial activity and associated iron (oxy)hydroxides are known in general to affect the environmental dynamics of 4-hydroxy-3-nitrobenzenearsonic acid (roxarsone), the mechanistic understanding of the underlying biophysico-chemical processes remains unclear due to limited experimental information. we studied how shewanella oneidensis mr-1 -a widely distributed metal-reducing bacterium, in the presence of dissolved fe(iii), affects roxarsone transformations and biogeochemical cycling i ...	2016	27100323
activation of an otherwise silent xylose metabolic pathway in shewanella oneidensis.	shewanella oneidensis is unable to metabolize the sugar xylose as a carbon and energy source. in the present study, an otherwise silent xylose catabolic pathway was activated in s. oneidensis by following an adaptive evolution strategy. genome-wide scans indicated that the s. oneidensis genome encoded two proteins similar to the xylose oxido-reductase pathway enzymes xylose reductase (so_0900) and xylulokinase (so_4230), and purified so_0900 and so_4230 displayed xylose reductase and xylulokinas ...	2016	27107127
potent antimicrobial and antibiofilm activities of bacteriogenically synthesized gold-silver nanoparticles against pathogenic bacteria and their physiochemical characterizations.	the objective of this study was to develop a bimetallic nanoparticle with enhanced antibacterial activity that would improve the therapeutic efficacy against bacterial biofilms. bimetallic gold-silver nanoparticles were bacteriogenically synthesized using γ-proteobacterium, shewanella oneidensis mr-1. the antibacterial activities of gold-silver nanoparticles were assessed on the planktonic and biofilm phases of individual and mixed multi-cultures of pathogenic gram negative (escherichia coli and ...	2016	27117745
evolved plasmid-host interactions reduce plasmid interference cost.	antibiotic selection drives adaptation of antibiotic resistance plasmids to new bacterial hosts, but the molecular mechanisms are still poorly understood. we previously showed that a broad-host-range plasmid was poorly maintained in shewanella oneidensis, but rapidly adapted through mutations in the replication initiation gene trfa1. here we examined if these mutations reduced the fitness cost of trfa1, and whether this was due to changes in interaction with the host's dna helicase dnab. the str ...	2016	27121483
mechanisms of bacterial extracellular electron exchange.	the biochemical mechanisms by which microbes interact with extracellular soluble metal ions and insoluble redox-active minerals have been the focus of intense research over the last three decades. the process presents two challenges to the microorganism. firstly, electrons have to be transported at the cell surface, which in gram-negative bacteria presents an additional problem of electron transfer across the ~6nm of the outer membrane. secondly, the electrons must be transferred to or from the ...	2016	27134022
k-shell analysis reveals distinct functional parts in an electron transfer network and its implications for extracellular electron transfer.	shewanella oneidensis mr-1 is capable of extracellular electron transfer (eet) and hence has attracted considerable attention. the eet pathways mainly consist of c-type cytochromes, along with some other proteins involved in electron transfer processes. by whole genome study and protein interactions inquisition, we constructed a large-scale electron transfer network containing 2276 interactions among 454 electron transfer related proteins in s. oneidensis mr-1. using the k-shell decomposition me ...	2016	27148219
a decahaem cytochrome as an electron conduit in protein-enzyme redox processes.	the decahaem cytochrome mtrc from shewanella oneidensis mr-1 was employed as a protein electron conduit between a porous indium tin oxide electrode and redox enzymes. using a hydrogenase and a fumarate reductase, mtrc was shown as a suitable and efficient diode to shuttle electrons to and from the electrode with the mtrc redox activity regulating the direction of the enzymatic reactions.	2016	27193068
contribution of direct electron transfer mechanisms to overall electron transfer in microbial fuel cells utilising shewanella oneidensis as biocatalyst.	to investigate the contribution of direct electron transfer mechanisms to electricity production in microbial fuel cells by physically retaining shewanella oneidensis cells close to or away from the anode electrode.	2016	27193895
effects of incubation conditions on cr(vi) reduction by c-type cytochromes in intact shewanella oneidensis mr-1 cells.	it is widely recognized that the outer membrane c-type cytochromes (om c-cyts) of metal-reducing bacteria play a key role in microbial metal reduction processes. however, the in situ redox status of om c-cyts during microbial metal reduction processes remain poorly understood. in this study, diffuse-transmission uv/vis spectroscopy is used to investigate the in situ spectral reaction of cr(vi) reduction by c-cyts in intact shewanella oneidensis mr-1 cells under different incubation conditions. t ...	2016	27242759
modeling of sustainable base production by microbial electrolysis cell.	a predictive model for the microbial/electrochemical base formation from wastewater was established and compared to experimental conditions within a microbial electrolysis cell. a na2 so4 /k2 so4 anolyte showed that model prediction matched experimental results. using shewanella oneidensis mr-1, a strong base (ph≈13) was generated using applied voltages between 0.3 and 1.1 v. due to the use of bicarbonate, the ph value in the anolyte remained unchanged, which is required to maintain microbial ac ...	2016	27265318
bound flavin-cytochrome model of extracellular electron transfer in shewanella oneidensis: analysis by free energy molecular dynamics simulations.	flavins are known to enhance extracellular electron transfer (eet) in shewanella oneidensis mr-1 bacteria, which reduce electron acceptors through outer-membrane (om) cytochromes c. free-shuttle and bound-redox cofactor mechanisms were proposed to explain this enhancement, but recent electrochemical reports favor a flavin-bound model, proposing two one-electron reductions of flavin, namely, oxidized (ox) to semiquinone (sq) and semiquinone to hydroquinone (hq), at anodic and cathodic conditions, ...	2016	27266856
bacteria-affinity 3d macroporous graphene/mwcnts/fe3o4 foams for high-performance microbial fuel cells.	promoting the performance of microbial fuel cells (mfcs) relies heavily on the structure design and composition tailoring of electrode materials. in this work, three-dimensional (3d) macroporous graphene foams incorporated with intercalated spacer of multiwalled carbon nanotubes (mwcnts) and bacterial anchor of fe3o4 nanospheres (named as g/mwcnts/fe3o4 foams) were first synthesized and used as anodes for shewanella-inoculated microbial fuel cells (mfcs). thanks to the macroporous structure of 3 ...	2016	27266894
structures of kdnb and kdna from shewanella oneidensis: key enzymes in the formation of 8-amino-3,8-dideoxy-d-manno-octulosonic acid.	8-amino-3,8-dideoxy-d-manno-octulosonic acid (kdo8n) is a unique amino sugar that has thus far only been observed on the lipopolysaccharides of marine bacteria belonging to the genus shewanella. although its biological function is still unclear, it is thought that the sugar is important for the integrity of the bacterial cell outer membrane. a three-gene cluster required for the biosynthesis of kdo8n was first identified in shewanella oneidensis. here we describe the three-dimensional structures ...	2016	27275764
efficient respiration on tmao requires tord and tore auxiliary proteins in shewanella oneidensis.	respiration on trimethylamine oxide (tmao) allows bacterial survival under anoxia. in shewanella oneidensis, tor is the system involved in tmao respiration and it is encoded by the torecad operon. the tora and torc genes encode tora terminal reductase and the torc c-type cytochrome, respectively. sequence analysis suggests that tord is the putative specific chaperone of tora, whereas tore is of unknown function. the purpose of this study was to understand whether tord and tore are two accessory ...	2016	27288570
iron isotope fractionations reveal a finite bioavailable fe pool for structural fe(iii) reduction in nontronite.	we report on stable fe isotope fractionation during microbial and chemical reduction of structural fe(iii) in nontronite nau-1. (56)fe/(54)fe fractionation factors between aqueous fe(ii) and structural fe(iii) ranged from -1.2 to +0.8‰. microbial (shewanella oneidensis and geobacter sulfurreducens) and chemical (dithionite) reduction experiments revealed a two-stage process. stage 1 was characterized by rapid reduction of a finite fe(iii) pool along the edges of the clay particles, accompanied b ...	2016	27291525
atp and autophosphorylation driven conformational changes of hipa kinase revealed by ion mobility and crosslinking mass spectrometry.	toxin-antitoxin systems are genetic modules involved in a broad range of bacterial cellular processes including persistence, multidrug resistance and tolerance, biofilm formation, and pathogenesis. in type ii toxin-antitoxin systems, both the toxin and antitoxin are proteins. in the prototypic escherichia coli hipa-hipb module, the antitoxin hipb forms a complex with the protein kinase hipa and sequesters it in the nucleoid. hipa is then no longer able to phosphorylate glutamyl-trna-synthetase a ...	2016	27325463
a solvent-free microbial-activated air cathode battery paper platform made with pencil-traced graphite electrodes.	we present the fabrication of an ultra-low cost, disposable, solvent-free air cathode all-paper microbial fuel cell (mfc) that does not utilize any chemical treatments. the anode and cathode were fabricated by depositing graphite particles by drawing them on paper with a pencil (four strokes). hydrophobic parchment paper was used as a proton exchange membrane (pem) to allow only h(+) to pass. air cathode mfc technology, where o2 was used as an electron acceptor, was implemented on the paper plat ...	2016	27333815
production of manganese oxide nanoparticles by shewanella species.	several species of the bacterial genus shewanella are well-known dissimilatory reducers of manganese under anaerobic conditions. in fact, shewanella oneidensis is one of the most well studied of all metal-reducing bacteria. in the current study, a number of shewanella strains were tested for manganese-oxidizing capacity under aerobic conditions. all were able to oxidize mn(ii) and to produce solid dark brown manganese oxides. shewanella loihica strain pv-4 was the strongest oxidizer, producing o ...	2016	27342559
regulation of gene expression in shewanella oneidensis mr-1 during electron acceptor limitation and bacterial nanowire formation.	in limiting oxygen as an electron acceptor, the dissimilatory metal-reducing bacterium shewanella oneidensis mr-1 rapidly forms nanowires, extensions of its outer membrane containing the cytochromes mtrc and omca needed for extracellular electron transfer. rna sequencing (rna-seq) analysis was employed to determine differential gene expression over time from triplicate chemostat cultures that were limited for oxygen. we identified 465 genes with decreased expression and 677 genes with increased ...	2016	27342561
effect of oxygen on the per-cell extracellular electron transfer rate of shewanella oneidensis mr-1 explored in bioelectrochemical systems.	extracellular electron transfer (eet) is a mechanism that enables microbes to respire solid-phase electron acceptors. these eet reactions most often occur in the absence of oxygen, since oxygen can act as a competitive electron acceptor for many facultative microbes. however, for shewanella oneidensis mr-1, oxygen may increase biomass development, which could result in an overall increase in eet activity. here, we studied the effect of oxygen on s. oneidensis mr-1 eet rates using bioelectrochemi ...	2017	27399911
glycerol-fed microbial fuel cell with a co-culture of shewanella oneidensis mr-1 and klebsiella pneumonae j2b.	glycerol is an attractive feedstock for bioenergy and bioconversion processes but its use in microbial fuel cells (mfcs) for electrical energy recovery has not been investigated extensively. this study compared the glycerol uptake and electricity generation of a co-culture of shewanella oneidensis mr-1 and klebsiella pneumonia j2b in a mfc with that of a single species inoculated counterpart. glycerol was metabolized successfully in the co-culture mfc (mfc-j&m) with simultaneous electricity prod ...	2016	27412724
direct conversion of cellulose and hemicellulose to fermentable sugars by a microbially-driven fenton reaction.	the aim of this work was to develop a microbially-driven fenton reaction that fragments cellulose and hemicellulose, degrades cellodextrins and xylodextrins, and produces short-chain oligosaccharides and monomeric sugars in a single bioreactor. the lignocellulose degradation system operates at neutral ph and does not require addition of conventional lignocellulose-degrading enzymes, thus avoiding problems associated with enzyme accessibility and specificity. the ability to produce useful bioprod ...	2016	27469094
cold adaptation regulated by cryptic prophage excision in shewanella oneidensis.	among the environmental stresses experienced by bacteria, temperature shifts are one of the most important. in this study, we discovered a novel cold adaptation mechanism in shewanella oneidensis that occurs at the dna level and is regulated by cryptic prophage excision. previous studies on bacterial cold tolerance mainly focus on the structural change of cell membrane and changes at the rna and protein levels. whether or not genomic change can also contribute to this process has not been explor ...	2016	27482926
molecular underpinnings of nitrite effect on cyma-dependent respiration in shewanella oneidensis.	shewanella exhibit a remarkable versatility of respiration, with a diverse array of electron acceptors (eas). in environments where these bacteria thrive, multiple eas are usually present. however, we know little about strategies by which these eas and their interaction affect ecophysiology of shewanella. in this study, we demonstrate in the model strain, shewanella oneidensis mr-1, that nitrite, not through nitric oxide to which it may convert, inhibits respiration of fumarate, and probably man ...	2016	27493647
biomanufacture of nano-pd(0) by escherichia coli and electrochemical activity of bio-pd(0) made at the expense of h2 and formate as electron donors.	palladised cells of desulfovibrio desulfuricans and shewanella oneidensis have been reported as fuel cell electrocatalysts but growth at scale may be unattractive/costly; we have evaluated the potential of using e. coli, using h2/formate for pd-nanoparticle manufacture.	2016	27502834
functional identification of putrescine c- and n-hydroxylases.	the small polyamine putrescine (1,4-diaminobutane) is ubiquitously and abundantly found in all three domains of life. it is a precursor, through n-aminopropylation or n-aminobutylation, for biosynthesis of the longer polyamines spermidine, sym-homospermidine, spermine, and thermospermine and longer and branched chain polyamines. putrescine is also biochemically modified for purposes of metabolic regulation and catabolism, e.g. n-acetylation and n-glutamylation, and for incorporation into special ...	2016	27541336
simultaneous transformation of commingled trichloroethylene, tetrachloroethylene, and 1,4-dioxane by a microbially driven fenton reaction in batch liquid cultures.	improper disposal of 1,4-dioxane and the chlorinated organic solvents trichloroethylene (tce) and tetrachloroethylene (also known as perchloroethylene [pce]) has resulted in widespread contamination of soil and groundwater. in the present study, a previously designed microbially driven fenton reaction system was reconfigured to generate hydroxyl (ho˙) radicals for simultaneous transformation of source zone levels of single, binary, and ternary mixtures of tce, pce, and 1,4-dioxane. the reconfigu ...	2016	27542932
in-situ growth of graphene/polyaniline for synergistic improvement of extracellular electron transfer in bioelectrochemical systems.	graphene composite has been widely used in various bioelectrochemical systems (bes). however, it is suffered from tedious fabrication procedure and ambiguous mechanism for its effect on bes. here, a one-step and in-situ strategy for simultaneously graphene exfoliation and aniline polymerization was developed for fabrication of graphene/pani composite electrode (go/panios). this go/panios outperformed graphite paper (gp), gp with pani (gp/pani) and gp with electrochemical exfoliated graphene (goh ...	2017	27566391
evolution of cell size homeostasis and growth rate diversity during initial surface colonization of shewanella oneidensis.	cell size control and homeostasis are fundamental features of bacterial metabolism. recent work suggests that cells add a constant size between birth and division ("adder" model). however, it is not known how cell size homeostasis is influenced by the existence of heterogeneous microenvironments, such as those during biofilm formation. shewanella oneidensis mr-1 can use diverse energy sources on a range of surfaces via extracellular electron transport (eet), which can impact growth, metabolism, ...	2016	27571459
suppression of fabb mutation by fabf1 is mediated by transcription read-through in shewanella oneidensis.	as type ii fatty acid synthesis is essential for the growth of escherichia coli, its many components are regarded as potential targets for novel antibacterial drugs. among them, β-ketoacyl-acyl carrier protein (acp) synthase (kas) fabb is the exclusive factor for elongation of the cis-3-decenoyl-acp (cis-3-c10-acp). in our previous study, we presented evidence to suggest that this may not be the case in shewanella oneidensis, an emerging model gammaproteobacterium renowned for its respiratory ve ...	2016	27573012
heterogeneity of the electron exchange capacity of kitchen waste compost-derived humic acids based on fluorescence components.	composting is widely used for recycling of kitchen waste to improve soil properties, which is mainly attributed to the nutrient and structural functions of compost-derived humic acids (has). however, the redox properties of compost-derived has are not fully explored. here, a unique framework is employed to investigate the electron exchange capacity (eec) of has during kitchen waste composting. most components of compost-derived has hold eec, but nearly two-thirds of them are found to be easily d ...	2016	27580607
a synthetic microbial consortium of shewanella and bacillus for enhanced generation of bioelectricity.	in this study, a synthetic microbial consortium containing exoelectrogen shewanella oneidensis mr-1 and riboflavin-producing strain, bacillus subtilis rh33, was rationally designed and successfully constructed, enabling a stable, multiple cycles of microbial fuel cells (mfcs) operation for more than 500 h. the maximum power density of mfcs with this synthetic microbial consortium was 277.4 mw/m(2) , which was 4.9 times of that with mr-1 (56.9 mw/m(2) ) and 40.2 times of rh33 (6.9 mw/m(2) ), sepa ...	2017	27596754
an itraq characterisation of the role of tolc during electron transfer from shewanella oneidensis mr-1.	anodophilic bacteria have the ability to generate electricity in microbial fuel cells (mfcs) by extracellular electron transfer to the anode. we investigated the anode-specific responses of shewanella oneidensis mr-1, an exoelectroactive gammaproteobacterium, using for the first time itraq and 2d-lc ms/ms driven membrane proteomics to compare protein abundances in s. oneidensis when generating power in mfcs, and growing in a continuous culture. the regulated dataset produced was enriched in memb ...	2016	27599463
improving the molecular ion signal intensity for in situ liquid sims analysis.	in situ liquid secondary ion mass spectrometry (sims) enabled by system for analysis at the liquid vacuum interface (salvi) has proven to be a promising new tool to provide molecular information at solid-liquid and liquid-vacuum interfaces. however, the initial data showed that useful signals in positive ion spectra are too weak to be meaningful in most cases. in addition, it is difficult to obtain strong negative molecular ion signals when m/z>200. these two drawbacks have been the biggest obst ...	2016	27600576
anodic biofilms as the interphase for electroactive bacterial growth on carbon veil.	the structure and activity of electrochemically active biofilms (eabs) are usually investigated on flat electrodes. however, real world applications such as wastewater treatment and bioelectrosynthesis require tridimensional electrodes to increase surface area and facilitate eab attachment. the structure and activity of thick eabs grown on high surface area electrodes are difficult to characterize with electrochemical and microscopy methods. here, the authors adopt a stacked electrode configurat ...	2016	27609094
mutations targeting the plug-domain of the shewanella oneidensis proton-driven stator allow swimming at increased viscosity and under anaerobic conditions.	shewanella oneidensis mr-1 possesses two different stator units to drive flagellar rotation, the na+ -dependent pomab stator and the h+ -driven motab stator, the latter possibly acquired by lateral gene transfer. although either stator can independently drive swimming through liquid, motab-driven motors cannot support efficient motility in structured environments or swimming under anaerobic conditions. using δpomab cells we isolated spontaneous mutants able to move through soft agar. we show tha ...	2016	27611183
enrofloxacin transformation on shewanella oneidensis mr-1 reduced goethite during anaerobic-aerobic transition.	antibiotics pollution has become a critical environmental issue worldwide due to its high ecological risk. in this study, rapid degradation of enrofloxacin (enr) was observed on goethite in the presence of shewanella oneidensis mr-1 during the transition from anaerobic to aerobic conditions. the abiotic reactions also demonstrated that over 70% with initial concentration of 10 mg l-1 enr was aerobically removed within 5 min by goethite with adsorbed fe(ii), without especial irradiation and stron ...	2016	27635981
a re-evaluation of electron-transfer mechanisms in microbial electrochemistry: shewanella releases iron that mediates extracellular electron transfer.	exoelectrogenic bacteria can couple their metabolism to extracellular electron acceptors, including macroscopic electrodes, and this has applications in energy production, bioremediation and biosensing. optimisation of these technologies relies on a detailed molecular understanding of extracellular electron-transfer (eet) mechanisms, and shewanella oneidensis mr-1 (mr-1) has become a model organism for such fundamental studies. here, cyclic voltammetry was used to determine the relationship betw ...	2016	27668145
photoreduction of shewanella oneidensis extracellular cytochromes by organic chromophores and dye-sensitized tio2.	the transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar-assisted chemical synthesis. in shewanella oneidensis mr-1 (mr-1), trans-outer-membrane electron transfer is performed by the extracellular cytochromes mtrc and omca acting together with the outer-membrane-spanning porin⋅cytochrome complex (mtrab). here we demons ...	2016	27685371
oxygen tension and riboflavin gradients cooperatively regulate the migration of shewanella oneidensis mr-1 revealed by a hydrogel-based microfluidic device.	shewanella oneidensis is a model bacterial strain for studies of bioelectrochemical systems (bess). it has two extracellular electron transfer pathways: (1) shuttling electrons via an excreted mediator riboflavin; and (2) direct contact between the c-type cytochromes at the cell membrane and the electrode. despite the extensive use of s. oneidensis in bess such as microbial fuel cells and biosensors, many basic microbiology questions about s. oneidensis in the context of bes remain unanswered. h ...	2016	27703448
microbial synthesis of highly dispersed pdau alloy for enhanced electrocatalysis.	biosynthesis based on the reducing capacity of electrochemically active bacteria is frequently used in the reduction of metal ions into nanoparticles as an eco-friendly way to recycle metal resources. however, those bionanoparticles cannot be used directly as electrocatalysts because of the poor conductivity of cell substrates. this problem was solved by a hydrothermal reaction, which also contributes to the heteroatom doping and alloying between pd and au. with the protection of graphene, the a ...	2016	27704047
in situ molecular imaging of the biofilm and its matrix.	molecular mapping of live biofilms at submicrometer resolution presents a grand challenge. here, we present the first chemical mapping results of biofilm extracellular polymeric substance (eps) in biofilms using correlative imaging between super resolution fluorescence microscopy and liquid time-of-flight secondary ion mass spectrometry (tof-sims). shewanella oneidensis is used as a model organism. heavy metal chromate (cr2o72-) anions consisting of chromium cr(vi) was used as a model environmen ...	2016	27709903
comparative proteomics implicates a role for multiple secretion systems in electrode-respiring geobacter sulfurreducens biofilms.	geobacter sulfurreducens is a dissimilatory metal-reducing bacterium capable of forming thick electron-conducting biofilms on solid electrodes. here, we employ for the first time comparative proteomics to identify key physiological changes involved in g. sulfurreducens adaptation from fumarate-respiring planktonic cells to electron-conducting biofilms. increased levels of proteins involved in outer membrane biogenesis, cell motility and secretion are expressed in biofilms. of particular importan ...	2016	27718580
light-induced reduction of silver ions to silver nanoparticles in aquatic environments by microbial extracellular polymeric substances (eps).	microbial extracellular polymeric substances (eps) widely exist in natural environments and affect the migration and transformation of pollutants in aquatic environments. previous works report that eps have some reducing functional groups and can reduce heavy metals. however, because of the weak reducing capability of eps, the reduction of heavy metals by eps without cells is extremely slow, and its effect on heavy metals species is insignificant. in this work, the accelerated reduction of silve ...	2016	27728818
wiring bacterial electron flow for sensitive whole-cell amperometric detection of riboflavin.	a whole-cell bioelectrochemical biosensing system for amperometric detection of riboflavin was developed. a "bioelectrochemical wire" (bw) consisting of riboflavin and cytochrome c between shewanella oneidensis mr-1 and electrode was characterized. typically, a strong electrochemical response was observed when riboflavin (vb2) was added to reinforce this bw. impressively, the electrochemical response of riboflavin with this bw was over 200 times higher than that without bacteria. uniquely, this ...	2016	27750415
deletion of degq gene enhances outer membrane vesicle production of shewanella oneidensis cells.	shewanella oneidensis is a gram-negative facultative anaerobe that can use a wide variety of terminal electron acceptors for anaerobic respiration. in this study, s. oneidensis degq gene, encoding a putative periplasmic serine protease, was cloned and expressed. the activity of purified degq was inhibited by diisopropyl fluorophosphate, a typical serine protease-specific inhibitor, indicating that degq is a serine protease. in-frame deletion and subsequent complementation of the degq were carrie ...	2017	27796471
microbial mediated iron redox cycling in fe (hydr)oxides for nitrite removal.	nitrite, at an environmentally relevant concentration, was significantly reduced with iron (hydr)oxides mediated by shewanella oneidensis mr-1. the average nitrite removal rates of 1.28±0.08 and 0.65±0.02(mgl-1)h-1 were achieved with ferrihydrite and magnetite, respectively. the results showed that nitrite removal was able to undergo multiple redox cycles with iron (hydr)oxides mediated by shewanella oneidensis mr-1. during the bioreduction of the following cycles, biogenic fe(ii) was subsequent ...	2017	27806884
the general stress response σs is regulated by a partner switch in the gram-negative bacterium shewanella oneidensis.	here, we show that a partner-switching system of the aquatic proteobacterium shewanella oneidensis regulates post-translationally σ(s) (also called rpos), the general stress response sigma factor. genes so2118 and so2119 encode crsa and crsr, respectively. crsr is a three-domain protein comprising a receiver, a phosphatase, and a kinase/anti-sigma domains, and crsa is an anti-sigma antagonist. in vitro, crsr sequesters σ(s) and possesses kinase and phosphatase activities toward crsa. in turn, de ...	2016	27810894
modulation of the reactivity of multiheme cytochromes by site-directed mutagenesis: moving towards the optimization of microbial electrochemical technologies.	dissimilatory metal-reducing bacteria perform extracellular electron transfer, a metabolic trait that is at the core of a wide range of biotechnological applications. to better understand how these microorganisms transfer electrons from their metabolism to an extracellular electron acceptor, it is necessary to characterize in detail the key players in this process, the multiheme c-type cytochromes. shewanella oneidensis mr-1 is a model organism for studying extracellular electron transfer, where ...	2017	27817033
microbial fuel cells using cellulomonas spp. with cellulose as fuel.	cellulomonas fimi, cellulomonas biazotea, and cellulomonas flavigena are cellulose-degrading microorganisms chosen to compare the degradation of cellulose. c. fimi degraded 2.5 g/l of cellulose within 4 days, which was the highest quantity among the three microorganisms. the electric current generation by the microbial fuel cell (mfc) using the cellulose-containing medium with c. fimi was measured over 7 days. the medium in the mfc was sampled every 24 h to quantify the degradation of cellulose, ...	2017	27818074
boosting power density of microbial fuel cells with 3d nitrogen-doped graphene aerogel electrode.	a 3d nitrogen-doped graphene aerogel (n-ga) as an anode material for microbial fuel cells (mfcs) is reported. electron microscopy images reveal that the n-ga possesses hierarchical porous structure that allows efficient diffusion of both bacterial cells and electron mediators in the interior space of 3d electrode, and thus, the colonization of bacterial communities. electrochemical impedance spectroscopic measurements further show that nitrogen doping considerably reduces the charge transfer res ...	2016	27818911
a mechanistic study of tio2 nanoparticle toxicity on shewanella oneidensis mr-1 with uv-containing simulated solar irradiation: bacterial growth, riboflavin secretion, and gene expression.	toxicity of nanomaterials to ecological systems has recently emerged as an important field of research, and thus, many researchers are exploring the mechanisms of how nanoparticles impact organisms. herein, we probe the mechanisms of bacteria-nanoparticle interaction by investigating how tio2 nanoparticles impact a model organism, the metal-reducing bacterium shewanella oneidensis mr-1. in addition to examining the effect of tio2 exposure, the effect of synergistic simulated solar irradiation co ...	2017	27823777
sdr-elisa: ultrasensitive and high-throughput nucleic acid detection based on antibody-like dna nanostructure.	an ultrasensitive and high-throughput nucleic acid detection system, termed as strand displacement reaction-enzyme linked immunosorbent assay (sdr-elisa), has been developed on the basis of antibody-like dna nanostructures. three digoxigenin or biotin modified hairpin probes are utilized to construct antibody-like dna nanostructures that feature affinity toward streptavidin and anti-digoxigenin antibody via isothermal target-triggered sdr amplification. these antibody-like nanostructures have be ...	2017	27825888
rapid construction of a whole-genome transposon insertion collection for shewanella oneidensis by knockout sudoku.	whole-genome knockout collections are invaluable for connecting gene sequence to function, yet traditionally, their construction has required an extraordinary technical effort. here we report a method for the construction and purification of a curated whole-genome collection of single-gene transposon disruption mutants termed knockout sudoku. using simple combinatorial pooling, a highly oversampled collection of mutants is condensed into a next-generation sequencing library in a single day, a 30 ...	2016	27830751
bioreductive deposition of highly dispersed ag nanoparticles on carbon nanotubes with enhanced catalytic degradation for 4-nitrophenol assisted by shewanella oneidensis mr-1.	biogenetic nanomaterials research provides insights and valuable implications for the green synthesis of nanomaterials and auxiliary biodegradation behaviors. ag nanoparticles (ag nps) fabricated on multiwalled carbon nanotubes (mwnts) (ag/mwnts nanocomposites) are prepared in situ assisted by shewanella oneidensis mr-1 (s. oneidensis mr-1) that provide respiratory pathway to transmit electrons. the ag/mwnts nanocomposites are characterized by a scanning electron microscopy (sem), an energy disp ...	2017	27854056
napb in excess inhibits growth of shewanella oneidensis by dissipating electrons of the quinol pool.	shewanella, a group of ubiquitous bacteria renowned for respiratory versatility, thrive in environments where various electron acceptors (eas) of different chemical and physiological characteristics coexist. despite being extensively studied, we still know surprisingly little about strategies by which multiple eas and their interaction define ecophysiology of these bacteria. previously, we showed that nitrite inhibits growth of the genus representative shewanella oneidensis on fumarate and presu ...	2016	27857202
extracellular biosynthesis of copper sulfide nanoparticles by shewanella oneidensis mr-1 as a photothermal agent.	photothermal therapy (ptt) is a minimally invasive and effective cancer treatment method and has a great potential for innovating the conventional chemotherapy approaches. copper sulfide (cus) exhibits photostability, low cost, and high absorption in near infrared region, and is recognized as an ideal candidate for ptt. however, cus, as a photothermal agent, is usually synthesized with traditional chemical approaches, which require high temperature, additional stabilization and hydrophilic modif ...	2016	27866620
anaerobic reduction of 2,6-dinitrotoluene by shewanella oneidensis mr-1: roles of mtr respiratory pathway and nfnb.	dinitrotoluene (dnt) is a widely present pollutant in aquatic environments, and its biodegradation is an economically attractive way to effectively removal. in aquatic environments, the presence of electrochemically active bacteria (eab) could contribute to the anaerobic bioreduction of dnt. however, the mechanism behind such a biodegradation process at gene level remains to be further elucidated. in this work, the anaerobic reduction of 2,6-dinitrotoluene (2,6-dnt) by shewanella oneidensis mr-1 ...	2017	27869299
activity-based screening of metagenomic libraries for hydrogenase enzymes.	here we outline how to identify hydrogenase enzymes from metagenomic libraries through an activity-based screening approach. a metagenomic fosmid library is constructed in e. coli and the fosmids are transferred into a hydrogenase deletion mutant of shewanella oneidensis (δhyab) via triparental mating. if a fosmid exhibits hydrogen uptake activity, s. oneidensis' phenotype is restored and hydrogenase activity is indicated by a color change of the medium from yellow to colorless. this new method ...	2017	27900696
cation-limited kinetic model for microbial extracellular electron transport via an outer membrane cytochrome c complex.	outer-membrane c-type cytochrome (om c-cyt) complexes in several genera of iron-reducing bacteria, such as shewanella and geobacter, are capable of transporting electrons from the cell interior to extracellular solids as a terminal step of anaerobic respiration. the kinetics of this electron transport has implications for controlling the rate of microbial electron transport during bioenergy or biochemical production, iron corrosion, and natural mineral cycling. herein, we review the findings fro ...	2016	27924259
mechanism of fluorescence enhancement of biosynthesized xfe2o4-bifeo3 (x = cr, mn, co, or ni) membranes.	ferrites-bismuth ferrite is an intriguing option for medical diagnostic imaging device due to its magnetoelectric and enhanced near-infrared fluorescent properties. however, the embedded xfo nanoparticles are randomly located on the bfo membranes, making implementation in devices difficult. to overcome this, we present a facile bio-approach to produce xfe2o4-bifeo3 (xfo-bfo) (x = cr, mn, co, or ni) membranes using shewanella oneidensis mr-1. the perovskite bfo enhances the fluorescence intensity ...	2016	27928781
computing and applying atomic regulons to understand gene expression and regulation.	understanding gene function and regulation is essential for the interpretation, prediction, and ultimate design of cell responses to changes in the environment. an important step toward meeting the challenge of understanding gene function and regulation is the identification of sets of genes that are always co-expressed. these gene sets, atomic regulons (ars), represent fundamental units of function within a cell and could be used to associate genes of unknown function with cellular processes an ...	2016	27933038
redox properties of extracellular polymeric substances (eps) from electroactive bacteria.	although the capacity for electroactive bacteria to convert environmental metallic minerals and organic pollutants is well known, the role of the redox properties of microbial extracellular polymeric substances (eps) in this process is poorly understood. in this work, the redox properties of eps from two widely present electroactive bacterial strains (shewanella oneidensis and pseudomonas putida) were explored. electrochemical analysis demonstrates that the eps extracted from the two strains exh ...	2016	27991531
effective methods for extracting extracellular polymeric substances from shewanella oneidensis mr-1.	extracellular polymeric substances (eps) play crucial roles in bio-aggregate formation and survival of bacterial cells. to develop an effective but harmless method for eps extraction from shewanella oneidensis mr-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °c), and treatments with h2so4, ethylenediaminetetraacetic acid (edta) and naoh, were examined, respectively. results from scanning electron microscope and flow cytometric analyses indicate that mr-1 ...	2016	27997408
the detoxification and degradation of benzothiazole from the wastewater in microbial electrolysis cells.	in this study, the high-production-volume chemical benzothiazole (bth) from synthetic water was fully degraded into less toxic intermediates of simple organic acids using an up-flow internal circulation microbial electrolysis reactor (uicmer) under the hydraulic retention time (hrt) of 24 h. the bioelectrochemical system was operated at 25 ± 2 °c and continuous-flow mode. the bth loading rate varied during experiments from 20 g·m(-3)·day(-1) to 110 g·m(-3)·day(-1). bth and soluble cod (chemical ...	2016	27999421
microbial synthesis of bimetallic pdpt nanoparticles for catalytic reduction of 4-nitrophenol.	bimetallic nanoparticles are generally believed to have improved catalytic activity and stability due to geometric and electronic changes. in this work, biogenic-pd (bio-pd), biogenic-pt (bio-pt), and biogenic-pdpt (bio-pdpt) nanoparticles were synthesized by shewanella oneidensis mr-1 in the absence or presence of quinone. compared with direct microbial reduction process, the addition of anthraquinone-2,6-disulfonate (aqds) could promote the reduction efficiency of pd(ii) or/and pt(iv) and resu ...	2017	28004366
biological recovery of platinum complexes from diluted aqueous streams by axenic cultures.	the widespread use of platinum in high-tech and catalytic applications has led to the production of diverse pt loaded wastewaters. effective recovery strategies are needed for the treatment of low concentrated waste streams to prevent pollution and to stimulate recovery of this precious resource. the biological recovery of five common environmental pt-complexes was studied under acidic conditions; the chloro-complexes ptcl42- and ptcl62-, the amine-complex pt(nh3)4cl2 and the pharmaceutical comp ...	2017	28046131
acetoin production via unbalanced fermentation in shewanella oneidensis.	this study describes the realization of an anoxic acetoin production process using the proteobacterium shewanella oneidensis. fermentative processes are of high biotechnological relevance since they offer high productivity and a low percentage of substrate consumption for anabolic processes. nevertheless, the range of compounds that can be produced as sole end product of a fermentative process is limited, since the average oxidation state of substrate and products has to be identical in the abse ...	2017	28059435
transcriptome and metabolome responses of shewanella oneidensis mr-1 to methyl orange under microaerophilic and aerobic conditions.	shewanella oneidensis mr-1 degrades various azo dyes under microaerophilic and anaerobic conditions, but this process is inhibited under aerobic conditions. the mechanisms underlying azo dye biodegradation and inhibition remain unknown. therefore, we investigated metabolic and transcriptional changes in strain mr-1, which was cultured under different conditions, to elucidate these mechanisms. at the transcriptional level, genes involved in certain metabolic processes, particularly the tricarboxy ...	2017	28070664
quantification of free polyelectrolytes present in colloidal suspension, revealing a source of toxic responses for polyelectrolyte-wrapped gold nanoparticles.	polyelectrolyte (pe) wrapping of colloidal nanoparticles (nps) is a standard method to control np surface chemistry and charge. because excess polyelectrolytes are usually employed in the surface modification process, it is critical to evaluate different purification strategies to obtain a clean final product and thus avoid ambiguities in the source of effects on biological systems. in this work, 4 nm diameter gold nanoparticles (aunps) were wrapped with 15 kda poly(allylamine hydrochloride) (pa ...	2017	28078889
resilience, dynamics, and interactions within a model multispecies exoelectrogenic-biofilm community.	anode-associated multispecies exoelectrogenic biofilms are essential for the function of bioelectrochemical systems (bess). the individual activities of anode-associated organisms and physiological responses resulting from coculturing are often hard to assess due to the high microbial diversity in these systems. therefore, we developed a model multispecies biofilm comprising three exoelectrogenic proteobacteria, shewanella oneidensis, geobacter sulfurreducens, and geobacter metallireducens, with ...	2017	28087529
a near-infrared light responsive c-di-gmp module-based and logic gate in shewanella oneidensis.	a novel, biofilm-based and logic gate was constructed in shewanella oneidensis through a near-infrared (nir) light responsive c-di-gmp module. the logic gate was demonstrated in microbial fuel cells with isopropyl β-d-thiogalactoside (iptg) and nir light as the inputs and electrical signals as the output.	2017	28098272
cometabolic degradation of chloramphenicol via a meta-cleavage pathway in a microbial fuel cell and its microbial community.	the performance of a microbial fuel cell (mfc) in terms of degradation of chloramphenicol (cap) was investigated. approximately 84% of 50mg/l cap was degraded within 12h in the mfc. a significant interaction of ph, temperature, and initial cap concentration was found on removal of cap, and a maximum degradation rate of 96.53% could theoretically be achieved at 31.48°c, a ph of 7.12, and an initial cap concentration of 106.37mg/l. moreover, cap was further degraded through a ring-cleavage pathway ...	2017	28110226
comparative stability of major facilitator superfamily transport proteins.	membrane transporters are a vital class of proteins for which there is little available structural and thermodynamic information. the major facilitator superfamily (mfs) is a large group of transport proteins responsible for transporting a wide range of substrates in eukaryotes and prokaryotes. we have used far-uv circular dichroism (cd) to assess whether transporters from this superfamily have the same chemical and thermal stability. we have compared the stability of five different mfs transpor ...	2017	28116476
integrated microfluidic flow-through microbial fuel cells.	this paper reports on a miniaturized microbial fuel cell with a microfluidic flow-through configuration: a porous anolyte chamber is formed by filling a microfluidic chamber with three-dimensional graphene foam as anode, allowing nutritional medium to flow through the chamber to intimately interact with the colonized microbes on the scaffolds of the anode. no nutritional media flow over the anode. this allows sustaining high levels of nutrient utilization, minimizing consumption of nutritional s ...	2017	28120875
a class c radical s-adenosylmethionine methyltransferase synthesizes 8-methylmenaquinone.	the membranous quinone/quinol pool is essential to the majority of life forms and has been widely used as an important biomarker in microbial taxonomy. in the anaerobic world, the most important quinones are menaquinone (mk) and a methylated form of mk, designated methylmenaquinone (mmk), which is anticipated to serve specifically in low-potential electron transport chains involved in anaerobic respiration. however, it has remained unclear how mmk is generated. here, we show that a novel enzyme ...	2017	28164386
nitric oxide-induced conformational changes govern h-nox and histidine kinase interaction and regulation in shewanella oneidensis.	nitric oxide (no) is implicated in biofilm regulation in several bacterial families via heme-nitric oxide/oxygen binding (h-nox) protein signaling. shewanella oneidensis h-nox (so h-nox) is associated with a histidine kinase (so hnok) encoded on the same operon, and together they form a multicomponent signaling network whereby the no-bound state of so h-nox inhibits so hnok autophosphorylation activity, affecting the phosphorylation state of three response regulators. although the conformational ...	2017	28170222
investigation concerning the formation process of gold nanoparticles by shewanella oneidensis mr-1.	shewanella oneidensis mr-1 is a facultative anaerobic bacterium that is known to transfer electrons generated during metabolism to various metal ions and produce nanoparticles on the bacterial surface. in this study, we tracked the formation of gold nanoparticles (au nps) on the s. oneidensis cell surfaces and investigated the roles of membrane proteins and extracellular polysaccharides in this process by spectrometry, zeta potential analysis, and electron microscopy.	2017	28190828
loss of oxyr reduces efficacy of oxygen respiration in shewanella oneidensis.	in many bacteria, oxyr is the major regulator controlling cellular response to h2o2. a common phenotype resulting from oxyr loss is reduced growth rate, but the underlying mechanism is unknown. we demonstrated in shewanella oneidensis, an important research model for applied and environmental microbes, that the defect is primarily due to an electron shortage to major terminal oxidase cytochrome cbb3. the loss of oxyr leads to enhanced production of electron carriers that compete for electrons ag ...	2017	28195212
high performance reduction of h2o2 with an electron transport decaheme cytochrome on a porous ito electrode.	the decaheme cytochrome mtrc from shewanella oneidensis mr-1 immobilized on an ito electrode displays unprecedented h2o2 reduction activity. although mtrc showed lower peroxidase activity in solution compared to horseradish peroxidase, the ten heme cofactors enable excellent electronic communication and a superior activity on the electrode surface. a hierarchical ito electrode enabled optimal immobilization of mtrc and a high current density of 1 ma cm(-2) at 0.4 v vs she could be obtained at ph ...	2017	28221032
the role of riboflavin in decolourisation of congo red and bioelectricity production using shewanella oneidensis-mr1 under mfc and non-mfc conditions.	dissimilatory metal reducing bacteria can exchange electrons extracellularly and hold great promise for their use in simultaneous wastewater treatment and electricity production. this study investigated the role of riboflavin, an electron carrier, in the decolourisation of congo red in microbial fuel cells (mfcs) using shewanella oneidensis mr-1 as a model organism. the contribution of the membrane-bound protein mtrc to the decolourisation process was also investigated. within the range of ribof ...	2017	28229332
enhanced photovoltage response of hematite-x-ferrite interfaces (x = cr, mn, co, or ni).	high-fluorescent p-x-ferrites (xfe2o4; xfo; x = fe, cr, mn, co, or ni) embedded in n-hematite (fe2o3) surfaces were successfully fabricated via a facile bio-approach using shewanella oneidensis mr-1. the results revealed that the x ions with high/low work functions modify the unpaired spin fe2+-o2- orbitals in the xfe2o4 lattices to become localized paired spin orbitals at the bottom of conduction band, separating the photovoltage response signals (73.36~455.16/-72.63~-32.43 mev). these (fe2o3)- ...	2017	28235371
enhanced photocurrent production by the synergy of hematite nanowire-arrayed photoanode and bioengineered shewanella oneidensis mr-1.	coupling the light-harvesting capabilities of semiconductors with the catalytic power of bacteria is a promising way to increase the efficiency of bioelectrochemical systems. here, we reported the enhanced photocurrents produced by the synergy of hematite nanowire-arrayed photoanode and the bio-engineered shewanella oneidensis mr-1 in a solar-assisted microbial photoelectrochemical system (solar mps) under the visible light. to increase the supply of bioelectrons, the d-lactate transporter, so15 ...	2017	28285200
growth trade-offs accompany the emergence of glycolytic metabolism in shewanella oneidensis mr-1.	bacteria increase their metabolic capacity via the acquisition of genetic material or by the mutation of genes already present in the genome. here, we explore the mechanisms and trade-offs involved when shewanella oneidensis, a bacterium that typically consumes small organic and amino acids, rapidly evolves to expand its metabolic capacity to catabolize glucose after a short period of adaptation to a glucose-rich environment. using whole-genome sequencing and genetic approaches, we discovered th ...	2017	28289083
electrode-assisted acetoin production in a metabolically engineered escherichia coli strain.	this paper describes the metabolic engineering of escherichia coli for the anaerobic fermentation of glucose to acetoin. acetoin has well-established applications in industrial food production and was suggested to be a platform chemical for a bio-based economy. however, the biotechnological production is often hampered by the simultaneous formation of several end products in the absence of an electron acceptor. moreover, typical production strains are often potentially pathogenic. the goal of th ...	2017	28293295
electrogenic single-species biocomposites as anodes for microbial fuel cells.	integration of electrogenic microorganisms remains a challenge in biofuel cell technology. here, synthetic biocomposites ("artificial biofilms") are proposed. bacteria (shewanella oneidensis) are embedded in a hydrogel matrix (poly(vinyl alcohol)) via wet- and electrospinning, creating fibers and nonwoven gauzes. the bacteria remain viable and metabolically active. the performance is compared to s. oneidensis suspension cultures and "natural" biofilms. while lower than with the suspension cultur ...	2017	28337840
a genome-scale model of shewanella piezotolerans simulates mechanisms of metabolic diversity and energy conservation.	shewanella piezotolerans strain wp3 belongs to the group 1 branch of the shewanella genus and is a piezotolerant and psychrotolerant species isolated from the deep sea. in this study, a genome-scale model was constructed for wp3 using a combination of genome annotation, ortholog mapping, and physiological verification. the metabolic reconstruction contained 806 genes, 653 metabolites, and 922 reactions, including central metabolic functions that represented nonhomologous replacements between the ...	2017	28382331
dynamics in the dual fuel flagellar motor of shewanella oneidensis mr-1.	the stator is an eminent component of the flagellar motor and determines a number of the motor's properties, such as the rotation-energizing coupling ion (h+ or na+) or the torque that can be generated. the stator consists of several units located in the cytoplasmic membrane surrounding the flagellar drive shaft. studies on flagellar motors of several bacterial species have provided evidence that the number as well as the retention time of stators coupled to the motor is highly dynamic and depen ...	2017	28389963
enhancing extracellular electron transfer of shewanella oneidensis mr-1 through coupling improved flavin synthesis and metal-reducing conduit for pollutant degradation.	dissimilatory metal reducing bacteria (dmrb) are capable of extracellular electron transfer (eet) to insoluble metal oxides, which are used as external electron acceptors by dmrb for their anaerobic respiration. the eet process has important contribution to environmental remediation mineral cycling, and bioelectrochemical systems. however, the low eet efficiency remains to be one of the major bottlenecks for its practical applications for pollutant degradation. in this work, shewanella oneidensi ...	2017	28414427
electrode assisted production of chemicals using shewanella oneidensis.		2017	28425632
starch-fueled microbial fuel cells by two-step and parallel fermentation using shewanella oneidensis mr-1 and streptococcus bovis 148.	shewanella oneidensis mr-1 generates electricity from lactic acid, but cannot utilize starch. on the other hand, streptococcus bovis 148 metabolizes starch and produces lactic acid. therefore, two methods were trialed for starch-fueled microbial fuel cell (mfc) in this study. in electric generation by two-step fermentation (egt) method, starch was first converted to lactic acid by s. bovis 148. the s. bovis 148 were then removed by centrifugation, and the fermented broth was preserved for electr ...	2017	28434977
anaerobic respiration on self-doped conjugated polyelectrolytes: impact of chemical structure.	we probe anaerobic respiration of bacteria in the presence of conjugated polyelectrolytes (cpes). three different cpes were used to probe how structural variations impact biocurrent generation from shewanella oneidensis mr-1. for the self-doped anionic cpe only, absorption spectroscopy shows that the addition of s. oneidensis mr-1 leads to the disappearance of the polaron (radical cation) band at >900 nm and an increase in the band at 735 nm due to the neutral species, consistent with electron t ...	2017	28444923
hsp90 is essential under heat stress in the bacterium shewanella oneidensis.	the hsp90 chaperone is essential in eukaryotes and activates a large array of client proteins. in contrast, its role is still elusive in bacteria, and only a few hsp90 bacterial clients are known. here, we found that hsp90 is essential in the model bacterium shewanella oneidensis under heat stress. a genetic screen for hsp90 client proteins identified tils, an essential protein involved in trna maturation. overexpression of tils rescued the growth defect of the hsp90 deletion strain under heat s ...	2017	28445720