| temporal separation of steps in the biosynthesis of verruculogen. | a mixture of 2[3h] mevalonate and u[14c] proline, given to penicillium simplicissimum on days 2, 3, 4, 5 or 6 of stationary liquid culture, gave rise to radiolabelled verruculogen in which the ratio of 3h to 14c measured on day 8 increased from 2.0:1 to 9.5:1 over the period of administration of radiolabelled precursors. this suggests temporal separation of the formation of the diketopiperazine moiety and its subsequent prenylation. the putative precursor diketopiperazine, cyclo-l-tryptophanyl-l ... | 1987 | 3453096 |
| biosynthesis of radiolabeled verruculogen by penicillium simplicissimum. | in surface culture of penicillium simplicissimum, verruculogen was shown to be biosynthesized from the intact carbon skeletons of tryptophan and proline, isoprenoid derivatives of mevalonic acid, and a methyl group donated by methionine. selected radiolabeled precursors (1 mci) pulse-fed at the optimum stage of fermentation yielded verruculogen (specific activity, 5.89 x 10(2) microci mmol-1) labeled in the prolyl and isoprenyl regions of the molecule and suitable for metabolic studies. | 1982 | 7041819 |
| isolation of penicillic acid from penicillium simplicissimum. | | 1969 | 5793329 |
| kojic acid from penicillium simplicissimum (oud.) thom. | | 1968 | 5660405 |
| induction of defense responses in cucumber plants by using the cell-free filtrate of the plant growth-promoting fungus penicillium simplicissimum gp17-2. | penicillium simplicissimum gp17-2 is a plant growth-promoting fungus (pgpf) and an inducer of systemic defense responses. the mechanisms underlying the effect of gp17-2 on the reduction of cucumber leaf damage caused by the anthracnose pathogen colletotrichum orbiculare were investigated. cucumber leaves treated with the culture filtrate (cf) of gp17-2 exhibited a clear systemic resistance against subsequent infection with c. orbiculare. the number and size of lesions caused by the disease were ... | 2013 | 23985491 |
| enzymatic modification of schizophyllan. | an enzymatic method was developed for the progressive modification of the polysaccharide schizophyllan. fungal strains hypocrea nigricans nrrl 62555, penicillium crustosum nrrl 62558, and penicillium simplicissimum nrrl 62550 were previously identified as novel sources of β-endoglucanase with specificity towards schizophyllan. concentrated enzyme preparations from these strains showed specific activities of 1.7-4.3 u β-glucanase/mg protein. using dilutions of these enzymes in time course digesti ... | 2015 | 25335747 |
| secreted xylanase xyna mediates utilization of xylan as sole carbon source in candida utilis. | the fodder yeast candida utilis is able to use xylose mono- and oligomers as sources of carbon but not the abundant polymer xylan. c. utilis transformants producing the penicillium simplicissimum xylanase xyna were constructed using expression vectors encoding fusions of the saccharomyces cerevisiae mfα1 pre-pro secretion leader to xyna. the mfα1-xyna fusion was efficiently processed in transformants and xyna was secreted almost quantitatively into the culture medium. secreted xyna was enzymatic ... | 2015 | 26051669 |
| fungal leaching of titanium from rock. | penicillium simplicissimum solubilized up to 80% of the titanium in granitic rocks but less than 2% of the titanium in basaltic rocks. | 1971 | 16349916 |
| fungal attack on rock: solubilization and altered infrared spectra. | penicillium simplicissimum, isolated from weathering basalt, produced citric acid when grown in a glucose-mineral salts medium with basalt, granite, granodiorite, rhyolite, andesite, peridotite, dunite, or quartzite. after 7 days' growth as much as 31 percent of the silicon, 11 percent of the aluminum, 64 percent of the iron, and 59 percent of the magnesium in some of the rocks were solubilized, and a number of rocks showed altered infrared absorption in the silicon-oxygen vibration region. | 1970 | 17838175 |
| purification and characterization of an intracellular catalase-peroxidase from penicillium simplicissimum. | the first dimeric catalase-peroxidase of eucaryotic origin, an intracellular hydroperoxidase from penicillium simplicissimum which exhibited both catalase and peroxidase activities, has been isolated. the enzyme has an apparent molecular mass of about 170 kda and is composed of two identical subunits. the purified protein has a ph optimum for catalase activity at 6.4 and for peroxidase at 5.4. both activities are inhibited by cyanide and azide whereas 3-amino-1,2,4-triazole has no effect. 3,3'-d ... | 1996 | 8631329 |
| substrate specificity of flavin-dependent vanillyl-alcohol oxidase from penicillium simplicissimum. evidence for the production of 4-hydroxycinnamyl alcohols from 4-allylphenols. | the substrate specificity of the flavoprotein vanillyl-alcohol oxidase from penicillium simplicissimum was investigated. vanillyl-alcohol oxidase catalyzes besides the oxidation of 4-hydroxybenzyl alcohols, the oxidative deamination of 4-hydroxybenzylamines and the oxidative demethylation of 4-(methoxymethyl)phenols. during the conversion of vanillylamine to vanillin, a transient intermediate, most probably vanillylimine, is observed. vanillyl-alcohol oxidase weakly interacts with 4-hydroxypheny ... | 1995 | 8529652 |