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identification of dehydrin-like proteins responsive to chilling in floral buds of blueberry (vaccinium, section cyanococcus).the level of three major polypeptides of 65, 60, and 14 kd increased in response to chilling unit accumulation in floral buds of a woody perennial, blueberry (vaccinium, section cynaococcus). the level of the polypeptides increased most dramatically within 300 h of chilling and decreased to the prechilling level with the initiation of budbreak. cold-hardiness levels were assessed for dormant buds of vaccinium corymbosum and vaccinium ashei after different chilling treatments until the resumption ...19948016270
antioxidant capacity, vitamin c, phenolics, and anthocyanins after fresh storage of small fruits.fresh strawberries (fragaria x ananassa duch.), raspberries (rubus idaeus michx.), highbush blueberries (vaccinium corymbosum l.), and lowbush blueberries (vaccinium angustifolium aiton) were stored at 0, 10, 20, and 30 degrees c for up to 8 days to determine the effects of storage temperature on whole fruit antioxidant capacity (as measured by the oxygen radical absorbing capacity assay, cao et al., clin. chem. 1995, 41, 1738-1744) and total phenolic, anthocyanin, and ascorbate content. the fou ...199910552863
effect of pollen load size and source (self, outcross) on seed and fruit production in highbush blueberry cv. 'bluecrop' (vaccinium corymbosum; ericaceae).reproductive fitness of a plant is ultimately determined by both number and quality of seed offspring. this is determined by sexual selection of pollen microspores and ovules during pollination and fertilization. these processes may include pollen competition and seed abortion, which reduce the number of microspores and ovules available for final seed production. thus, even an excess of pollen microspores to ovules does not result in fertile seeds equal to ovule number. we investigated pollen re ...200011080108
oxygen radical absorbance capacity (orac) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry.antioxidant capacity, as measured by oxygen radical absorbance capacity (orac), and total phenolic and total anthocyanin contents were evaluated in fruit tissues of 87 highbush blueberry (vacciniumcorymbosum l.) and species-introgressed highbush blueberry cultivars. orac and phenolic levels were evaluated in leaf tissues of the same materials. average values for orac, phenolics, and anthocyanins in fruit were 15.9 orac units, 1.79 mg/g (gallic acid equivalents), and 0.95 mg/g (cyanidin-3-glucosi ...200111368580
evaluation of various deployment strategies of imidacloprid-treated spheres in highbush blueberries for control of rhagoletis mendax (diptera: tephritidae).biodegradable, ammonium-baited spheres treated with the neonicotinoid insecticide provado (imidacloprid) at 2% (ai) were evaluated for controlling blueberry maggot flies, rhagoletis mendax curran. three strategies for sphere deployment in highbush blueberries, vaccinium corymbosum l., were compared with untreated control plots in 1999 and once again compared against control plots and organophosphate insecticide sprays in 2000. the patterns of sphere deployment were as follows: (1) perimeter depl ...200111561850
interspecific variation in anthocyanins, phenolics, and antioxidant capacity among genotypes of highbush and lowbush blueberries (vaccinium section cyanococcus spp.).recent interest in the possible protective effects of dietary antioxidant compounds against human degenerative disease has prompted investigation of foods such as blueberries (vaccinium sp.), which have a high antioxidant capacity. fruit obtained from genotypes of highbush blueberries (vaccinium corymbosum l.) and lowbush blueberries (vaccinium angustifolium aiton) were analyzed for their antioxidant capacity, their content of anthocyanins, and total phenolic compounds, to evaluate the intraspec ...200111600018
cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries.anthocyanins from tart cherries, prunus cerasus l. (rosaceae) cv. balaton and montmorency; sweet cherries, prunus avium l. (rosaceae); bilberries, vaccinum myrtillus l. (ericaceae); blackberries, rubus sp. (rosaceae); blueberries var. jersey, vaccinium corymbosum l. (ericaceae); cranberries var. early black, vaccinium macrocarpon ait. (ericaceae); elderberries, sambucus canadensis (caprifoliaceae); raspberries, rubus idaeus (rosaceae); and strawberries var. honeoye, fragaria x ananassa duch. (ro ...200111695879
native vaccinium spp. and gaylussacia spp. infested by rhagoletis mendax (diptera: tephritidae) in the great lakes region: a potential source of inoculum for infestation of cultivated blueberries.in this study, we addressed the question of whether or not native stands of blueberry (vaccinium spp.) and/or huckleberry (gaylussacia spp.) support populations of blueberry maggot, rhagoletis mendax curran, in the great lakes region. infestation of commercial blueberries by the blueberry maggot, r. mendax, is a serious problem in many areas where blueberries are grown. in the past 10-20 yr, commercial bighbush blueberry, vaccinium corymbosum l., production has expanded into places such as south ...200111777039
anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes.fruits from 107 genotypes of vaccinium l., rubus l., and ribes l., were analyzed for total anthocyanins (acy), total phenolics (tph), and antioxidant capacities as determined by oxygen radical absorbing capacity (orac) and ferric reducing antioxidant power (frap). fruit size was highly correlated (r = 0.84) with acy within vaccinium corymbosum l., but was not correlated to acy across eight other vaccinium species, or within 27 blackberry hybrids. certain vaccinium and ribes fruits with pigmented ...200211804523
oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries.the antioxidant activity of phenolics in fruits of blueberry (vaccinium corymbosum cv. sierra), cranberry (vaccinium macrocarpon cv. ben lear), wild chokeberry (aronia melanocarpa), and lingonberry (vaccinium vitis-idaea cv. amberland) was determined in this study. the phenolic constituents and contents among the different berries varied considerably. anthocyanins were found to be the main components in all these berries. chlorogenic acid in blueberry, quercetin glycosides in cranberry and lingo ...200312517117
anthocyanin and proanthocyanidin content in selected white and red wines. oxygen radical absorbance capacity comparison with nontraditional wines obtained from highbush blueberry.antioxidant capacity, as measured by oxygen radical absorbance capacity (orac(pe)), total phenolic, total and individual anthocyanins, and proanthocyanidin fraction contents were evaluated in red and white wines from grapes. a comparison in terms of antioxidant capacity is made with nontraditional wines made from highbush blueberry. blueberries are among fruits that are best recognized for their potential health benefits. in red wines, total oligomeric proanthocyanidin content, including catechi ...200312903941
resveratrol in raw and baked blueberries and bilberries.resveratrol in the fruits of bilberry (vaccinium myrtillus l.), the lowbush "wild" blueberry (vaccinium angustifolium aiton), the rabbiteye blueberry (vaccinium ashei reade), and the highbush blueberry (vaccinium corymbosum l.) were measured using a new assay based on high-performance liquid chromatography-tandem mass spectrometry (lc-ms/ms). the lc-ms/ms assay provided lower limits of detection than previous methods for resveratrol measurement, 90 fmol of trans-resveratrol injected on-column, a ...200313129286
effect of high-oxygen atmospheres on blueberry phenolics, anthocyanins, and antioxidant capacity.the influence of high oxygen concentrations on total phenolic, total anthocyanin, individual phenolic compounds, and antioxidant capacity (measured as oxygen radical absorbance capacity, orac) in highbush blueberry fruit (vaccinium corymbosum l. cv. duke) was investigated. freshly harvested blueberries were placed in jars ventilated continuously with air or with 40, 60, 80, or 100% o(2) at 5 degrees c for up to 35 days. samples were taken initially and at 7-day intervals during storage. whereas ...200314611188
resveratrol, pterostilbene, and piceatannol in vaccinium berries.a study was conducted to determine the presence of resveratrol, pterostilbene, and piceatannol in vaccinium berries. samples representing selections and cultivars of 10 species from mississippi, north carolina, oregon, and canada were analyzed by gas chromatography/mass spectrometry. resveratrol was found in vaccinium angustifolium (lowbush blueberry), vaccinium arboretum (sparkleberry), vaccinium ashei (rabbiteye blueberry), vaccinium corymbosum (highbush blueberry), vaccinium elliottii (elliot ...200415264904
agrobacterium tumefaciens-mediated transformation of blueberry (vaccinium corymbosum l.).transient expression studies using blueberry leaf explants and monitored by beta-glucuronidase (gus) assays indicated agrobacterium tumefaciens strain eha105 was more effective than lba4404 or gv3101; and the use of acetosyringone (as) at 100 microm for inoculation and 6 days co-cultivation was optimum compared to 2, 4, 8, 10 or 12 days. subsequently, explants of the cultivars aurora, bluecrop, brigitta, and legacy were inoculated with strain eha105 containing the binary vector pbisn1 with the n ...200415300402
the change of total anthocyanins in blueberries and their antioxidant effect after drying and freezing.this study examined the effects of freezing, storage, and cabinet drying on the anthocyanin content and antioxidant activity of blueberries (vaccinium corymbosum l). fresh samples were stored for two weeks at $\5^\circ$ c while frozen samples were kept for up to three months at $-20^\circ$ c. there were two drying treatments, one including osmotic pretreatment followed by cabinet drying and the other involving only cabinet drying. total anthocyanins found in fresh blueberries were $7.2 \pm 0.5$ ...200415577185
phenology and infestation patterns of plum curculio (coleoptera: curculionidae) on four highbush blueberry cultivars.the plum curculio, conotrachelus nenuphar (herbst), is a well known pest in apple and peach orchards, but it also is capable of having an economic impact in highbush blueberries. host phenology and plum curculio oviposition patterns were determined on four highbush blueberry cultivars differing in fruit maturation period. numbers of oviposition scars were higher on early- ('weymouth') and mid-season ('duke' and 'bluecrop') blueberries than on late-season 'elliott' in 2001, 2002, and 2003. in 200 ...200415666742
isoprene derivatives from the leaves and callus cultures of vaccinium corymbosum var. bluecrop.the phytochemical analysis of vaccinium corymbosum var bluecrop leaves and callus biomass revealed ursolic acid, oleanolic acid, alpha-amyrin and beta-amyrin in both plant materials. beta-sitosterol was determined only in callus biomass. the structure of isolated compounds was elucidated by tlc co-chromatography with standards and with spectroscopic methods (1h nmr, 13c nmr, ei-ms).201216022493
in vitro antileukaemic activity of extracts from berry plant leaves against sensitive and multidrug resistant hl60 cells.the aim of the present study was to determine in vitro antileukaemic activity of extracts obtained from selected berry plant leaves (fragaria x ananassa duch. cv elsanta, raspberry rubus ideus l. cv polana and blueberry vaccinium corymbosum l. cv bluecrop) against promyelocytic hl60 cell line and its multidrug resistant sublines exhibiting two different mdr phenotypes: hl60/vinc (overexpressing p-glycoprotein) and hl60/dox (overexpressing mrp1 protein). it was found that the blueberry extract wa ...200616039042
evaluation of vaccinium spp. for illinoia pepperi (hemiptera: aphididae) performance and phenolic content.host acceptance and population parameters of the aphid illinoia pepperi (macgillivray) (hemiptera: aphididae) were measured on highbush blueberry, vaccinium corymbosum l. 'elliott', and the wild species vaccinium boreale hall and aalders, vaccinium tenellum aiton, vaccinium pallidum aiton, vaccinium hirsutum buckley, vaccinium myrsinites lamarck, and vaccinium darrowi camp. after 24 h of exposure, significantly fewer aphids remained in contact with v. boreale and v. hirsutum compared with v. cor ...200616937706
major differences observed in transcript profiles of blueberry during cold acclimation under field and cold room conditions.our laboratory has been working toward increasing our understanding of the genetic control of cold hardiness in blueberry (vaccinium section cyanococcus) to ultimately use this information to develop more cold hardy cultivars for the industry. here, we report using cdna microarrays to monitor changes in gene expression at multiple times during cold acclimation under field and cold room conditions. microarrays contained over 2,500 cdna inserts, approximately half of which had been picked and sing ...200716953429
protective activities of vaccinium antioxidants with potential relevance to mitochondrial dysfunction and neurotoxicity.both the neurotransmitter dopamine (da) and a neurotoxic metabolite, 6-hydroxy da, can be oxidized to generate hydrogen peroxide and other reactive species (ros). ros promote oxidative stress and have been implicated in dopaminergic neurodegeneration, e.g., parkinson's disease (pd). there is also evidence for a relation between catecholamine-mediated oxidative damage in dopaminergic neurons and the effects of these neurotransmitters on the redox state of cytochrome c (cytc). in neurons and other ...200716956663
the influence of auxins on the biosynthesis of isoprene derivatives in callus cultures of vaccinium corymbosum var. bluecrop.callus cultures of vaccinium corymbosum var. bluecrop were optimized for their isoprene derivatives production by supplementing schenk-hildebrandt (sh) medium with constant concentration of kinetin (2.32 microm) and two different amounts of selected auxins. every auxin, except for iba, used in 10-time higher concentration (2,4d, naa, iaa, noa) stimulated biosynthesis of beta-sitosterol and inhibited triterpene synthesis. quantitative analysis of isoprene derivatives in callus biomass collected o ...201316989318
blueberry (vaccinium corymbosum l.).recent advances in plant biotechnology have led to a reliable and reproductive method for genetic transformation of blueberry. these efforts built on previous attempts at transient and stable transformation of blueberry that demonstrated the potential of agrobacterium tumefaciens-mediated transformation, and as well, the difficulties of selecting and regenerating transgenic plants. as a prerequisite for successful stable transformation, efficient regeneration systems were required despite many r ...200617033069
intraspecific variation in aphid resistance and constitutive phenolics exhibited by the wild blueberry vaccinium darrowi.illinoia pepperi (macgillivray) infests cultivated highbush blueberries, vaccinium corymbosum l., in the northeastern united states. allopatric resistance to i. pepperi was examined in vaccinium darrowi camp, which evolved in the absence of i. pepperi in the southeastern u.s. v. corymbosum cv. "elliott", was used as a susceptible control. between population variability in i. pepperi resistance was assessed by measuring length of the prereproductive period, fecundity, and survivorship on 14 v. da ...200717333374
anti-inflammatory and antinociceptive properties of blueberry extract (vaccinium corymbosum).blueberries are among the edible fruits that are recognized best for their potential health benefits. the crude extract from vaccinium corymbosum was assessed in anti-inflammatory and antinociceptive models. the crude hydroalcoholic extract was administered orally at doses of 100, 200 or 300 mg kg (-1) for all the assays. in the carrageenan test, the crude extract reduced rat paw oedema by 9.8, 28.5 and 65.9%, respectively. for the histamine assay, the reductions of oedema were 70.1, 71.7 and 81 ...200717430644
seasonal variation of temperature response of respiration in invasive berberis thunbergii (japanese barberry) and two co-occurring native understory shrubs in a northeastern us deciduous forest.in the understory of a closed forest, plant growth is limited by light availability, and early leafing is proposed to be an important mechanism of plant invasion by providing a spring c "subsidy" when high light is available. however, studies on respiration, another important process determining plant net c gain, are rare in understory invasive plants. in this study, leaf properties and the temperature response of leaf respiration were compared between invasive berberis thunbergii, an early leaf ...200717609983
leaf phenology and seasonal variation of photosynthesis of invasive berberis thunbergii (japanese barberry) and two co-occurring native understory shrubs in a northeastern united states deciduous forest.early leafing and extended leaf longevity can be important mechanisms for the invasion of the forest understory. we compared the leaf phenology and photosynthetic characteristics of berberis thunbergii, an early leafing invasive shrub, and two co-occurring native species, evergreen kalmia latifolia and late leafing vaccinium corymbosum, throughout the 2004 growing season. berberis thunbergii leafed out 1 month earlier than v. corymbosum and approximately 2 weeks prior to the overstory trees. the ...200717647021
lethal and sublethal activities of imidacloprid contribute to control of adult japanese beetle in blueberries.field-based bioassays and residue profile analysis were used to determine the relative importance of lethal and sublethal effects of imidacloprid on adult japanese beetle, popillia japonica newman, in blueberries, vaccinium corymbosum l. field-based bioassays assessed adult mortality and knockdown, and fruit and leaf injury from japanese beetles exposed to 4-h and 7-d field-aged residues of imidacloprid, and the conventional insecticides azinphosmethyl and esfenvalerate. azinphosmethyl and imida ...200717972637
berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease.an overwhelming body of research has now firmly established that the dietary intake of berry fruits has a positive and profound impact on human health, performance, and disease. berry fruits, which are commercially cultivated and commonly consumed in fresh and processed forms in north america, include blackberry ( rubus spp.), black raspberry ( rubus occidentalis), blueberry ( vaccinium corymbosum), cranberry (i.e., the american cranberry, vaccinium macrocarpon, distinct from the european cranbe ...200818211023
identification of anthocyanins in the liver, eye, and brain of blueberry-fed pigs.dietary intervention with anthocyanins may confer benefits in brain function, including vision. research to date indicates that animals have only a limited capacity to absorb anthocyanins, compared to other types of flavonoids. pigs, which are a suitable model for human digestive absorption, were used to examine the deposition of anthocyanins in tissues including the liver, eye, and brain tissue. pigs were fed diets supplemented with 0, 1, 2, or 4% w/w blueberries ( vaccinium corymbosum l. 'jers ...200818211026
steam-blanched highbush blueberry (vaccinium corymbosum l.) juice: phenolic profile and antioxidant capacity in relation to cultivar selection.high-quality standards in blueberry juice can be obtained only taking into account fruit compositional variability and its preservation along the processing chain. in this work, five highbush blueberry cultivars from the same environmental growing conditions were individually processed into juice after an initial blanching step and the influence was studied of the cultivar on juice phenolic content, distribution and relative antioxidant activity, measured as scavenging capacity on the artificial ...200818370394
global warming and flowering times in thoreau's concord: a community perspective.as a result of climate change, many plants are now flowering measurably earlier than they did in the past. however, some species' flowering times have changed much more than others. data at the community level can clarify the variation in flowering responses to climate change. in order to determine how north american species' flowering times respond to climate, we analyzed a series of previously unstudied records of the dates of first flowering for over 500 plant taxa in concord, massachusetts, ...200818409423
primary and secondary parasitoids (hymenoptera) of aphids (hemiptera: aphididae) on blueberry and other vaccinium in the pacific northwest.blueberry scorch virus, a commercially important carlavirus in highbush blueberry, vaccinium corymbosum l., is vectored by aphids (hemiptera: aphididae). we surveyed the aphids, primary parasitoids (hymenoptera: aphelinidae, braconidae), and associated secondary parasitoids (hymenoptera: charipidae, megaspilidae, pteromalidae) on highbush blueberry and other vaccinium in the pacific northwest from 1995 to 2006, with samples concentrated in 2005 and 2006, to lay the groundwork for augmentative bi ...200818419919
increasing antioxidant activity and reducing decay of blueberries by essential oils.several naturally occurring essential oils including carvacrol, anethole, cinnamaldehyde, cinnamic acid, perillaldehyde, linalool, and p-cymene were evaluated for their effectiveness in reducing decay and increasing antioxidant levels and activities in 'duke' blueberries ( vaccinium corymbosum). carvacrol, anethole, and perillaldehyde showed the capability to promote total anthocyanins and total phenolics and enhance antioxidant activity in fruit tissues expressed as oxygen radical absorbance ca ...200818442248
fruit quality, antioxidant capacity, and flavonoid content of organically and conventionally grown blueberries.the effect of cultivation practices on fruit quality and antioxidant capacity in highbush blueberries var. bluecrop (vaccinium corymbosum l.) was evaluated from random samples of commercial late harvest fields in new jersey. results from this study showed that blueberry fruit grown from organic culture yielded significantly higher sugars (fructose and glucose), malic acid, total phenolics, total anthocyanins, and antioxidant activity (orac) than fruit from the conventional culture. in organicall ...200818590274
interactions between chill-hours and degree-days affect carpogenic germination in monilinia vaccinii-corymbosi.abstract the relationship of cumulative chill-hours (hours with a mean temperature <7.2 degrees c) and heating degree-days (base 7.2 degrees c) to carpogenic germination of pseudosclerotia of monilinia vaccinii-corymbosi, which causes mummy berry disease of blueberry, was investigated. in two laboratory experiments, pseudosclerotia collected from rabbiteye blueberry in georgia were conditioned at 5 to 6 degrees c for 26 to 1,378 h prior to placement in conditions favorable for germination and ap ...200118944281
transmission, field spread, cultivar response, and impact on yield in highbush blueberry infected with blueberry scorch virus.scorch disease caused by blueberry scorch virus (blsv) spreads rapidly and radially from foci of infection. healthy potted blueberry plants became infected when placed next to diseased field bushes from early may through mid-august. the aphid fimbriaphis fimbriata, collected from infected field bushes, transmitted blsv to healthy blueberry plants in controlled tests and was regarded as the most important means by which bushes in commercial fields became infected. the rate of spread in the symp-t ...200018944552
transmission and the role of honeybees in field spread of blueberry shock ilarvirus, a pollen-borne virus of highbush blueberry.abstract the rapid and random spread of blueberry shock ilarvirus (blshv) in commercial plantings suggested that insects played a role in transmission. transmission from infected field plants to trap plants only occurred during bloom, indicating that flowers were the avenue for infection and flower-visiting insects including pollinators were involved. trap plants readily became infected during bloom when a honeybee hive was included in cages with diseased field plants and trap plants. there was ...199918944785
antioxidant activity of blueberry fruit is impaired by association with milk.the antioxidant properties of dietary phenolics are believed to be reduced in vivo because of their affinity for proteins. in this study we assessed the bioavailability of phenolics and the in vivo plasma antioxidant capacity after the consumption of blueberries (vaccinium corymbosum l.) with and without milk. in a crossover design, 11 healthy human volunteers consumed either (a) 200 g of blueberries plus 200 ml of water or (b) 200 g of blueberries plus 200 ml of whole milk. venous samples were ...200919135520
herbivore-induced volatiles in the perennial shrub, vaccinium corymbosum, and their role in inter-branch signaling.herbivore feeding activates plant defenses at the site of damage as well as systemically. systemic defenses can be induced internally by signals transported via phloem or xylem, or externally transmitted by volatiles emitted from the damaged tissues. we investigated the role of herbivore-induced plant volatiles (hipvs) in activating a defense response between branches in blueberry plants. blueberries are perennial shrubs that grow by initiating adventitious shoots from a basal crown, which produ ...200919159981
patterns of nocturnal rehydration in root tissues of vaccinium corymbosum l. under severe drought conditions.although roots in dry soil layers are commonly rehydrated by internal hydraulic redistribution during the nocturnal period, patterns of tissue rehydration are poorly understood. rates of nocturnal rehydration were examined in roots of different orders in vaccinium corymbosum l. 'bluecrop' (northern highbush blueberry) grown in a split-pot system with one set of roots in relatively moist soil and the other set of roots in dry soil. vaccinium is noted for a highly branched and extremely fine root ...200919188275
phytoparasitic nematodes associated with three types of blueberries in arkansas.research and commercial blueberry plantings were sampled in october 1991 to determine the population densities and species of phytoparasitic nematodes associated with rabbiteye (vaccinium ashei), southern highbush (vaccinium sp.), and highbusb (v. corymbosum) blueberry cultivars and the sod middles between the blueberry rows. in the research planting at clarksville, arkansas, samples from the highbush cv. bluecrop, the southern highbush cv. cooper and gulf coast, and the sod middles had similar ...199419279961
description of the blueberry root-knot nematode, meloidogyne carolinensis n. sp.meloidogyne carolinensis n. sp. is described from cultivated highbush blueberry (cultivars derived from hybrids of vaccinium corymbosum l. and v. lamarckii camp) in north carolina. the perineal pattern of the female has a large cuticular ridge that surrounds the perivulval area, and the excretory pore is near the level of the base of the stylet. the stylet is 15.9 mum long and the knobs gradually merge with the shaft. the head shape and stylet morphology of the male are quite variable. the typic ...198219295713
abundance of icerya purchasi (hemiptera: margarodidae) and its parasitoid cryptochaetum iceryae (diptera: cryptochaetidae) in argentina blueberry crops.icerya purchasi maskell is a polyphagous scale pest of ornamentals and several other crops. it has two natural specialist enemies that maintain its populations at low levels: rodolia cardinalis (mulsant) and cryptochaetum iceryae (williston). i. purchasi was recently observed in blueberry crops (vaccinium corymbosum l.) in argentina. seven blueberry fields located in different agricultural landscapes were sampled from july 2006 to september 2008. i. purchasi was present only in the citrus areas ...200919389286
general method for extraction of blueberry anthocyanins and identification using high performance liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry.a systematic approach for optimizing the extraction and identification of anthocyanins from blueberries was explored using hplc-uv and hplc-esi-it-tof-ms. sample homogenization effects, extraction solvent selection, type of acid, and amount used in extraction solvent were investigated. a mixture of methanol:water:trifluoroacetic acid (70:30:1, v/v/v) was found to be the best solvent system for blueberry anthocyanin extraction. differences in total anthocyanin content due to commercial blueberry ...200919414178
monitoring and temperature-based prediction of the whitemarked tussock moth (lepidoptera: lymantriidae) in blueberry.larvae of the whitemarked tussock moth, orgyia leucostigma (j.e. smith) (lepidoptera: lymantriidae), defoliate and contaminate blueberries, vaccinium corymbosum l., in eastern north america, but infestations are often not detected until economic damage has been caused. to improve monitoring techniques and understand the phenology of the whitemarked tussock moth in blueberry, we compared four trap types and determined temperature-based phenology of this pest over two growing seasons. large delta ...200919449644
optimization of pheromone deployment for effective mating disruption of oriental beetle (coleoptera: scarabaeidae) in commercial blueberries.cost is a potential limiting factor in the adoption of mating disruption to control oriental beetle, anomala orientalis (waterhouse) (coleoptera: scarabaeidae). a 3-yr study was conducted in 1-ha blueberry, vaccinium corymbosum l., plots to test the possibility of lowering cost by reducing the number of point-source dispensers and pheromone [(z)-7-tetradecen-2-one] concentrations, while maintaining mating disruption. trap shutdown, as measured by the disruption index (di), caged females, and sen ...200919449647
genetic diversity of armillaria spp. infecting highbush blueberry in northern italy (trentino region).armillaria spp. are the causal agents of root rots of several woody plants, including highbush blueberry. since 2003, highbush blueberry plants infected by armillaria spp. have been found in valsugana valley, trentino region, northern italy. our aim was to identify the armillaria spp. involved in these infections, as well as possible sources of inoculum in blueberry fields. samples of armillaria spp. were collected from diseased blueberry plants in 13 infected blueberry fields, from bark spread ...200919453223
volatiles released from vaccinium corymbosum were attractive to aegorhinus superciliosus (coleoptera: curculionidae) in an olfactometric bioassay.the objective of this study was to evaluate the role of host volatiles in the relationship between a blueberry plant vaccinium corymbosum l. and the raspberry weevil aegorhinus superciliosus (guérin) (coleoptera: curculionidae), the principal pest of blueberry in the south of chile. volatiles from the aerial part of different phenological stages of the host were collected on porapak q and analyzed by coupled gas chromatography-mass spectrometry (gc-ms). several chemical groups were identified in ...200919508787
antioxidant capacity and polyphenolic content of blueberry (vaccinium corymbosum l.) leaf infusions.antioxidant capacity and polyphenolic content of leaf infusions prepared from six highbush blueberry cultivars (vaccinium corymbosum l.), one wild lowbush blueberry cultivar (vaccinium myrtillus l.), and one commercially available mix of genotypes were determined. in order to simulate household tea preparation conditions, infusions were prepared in water heated to 95 degrees c. the dynamics of extraction of polyphenolic antioxidants were monitored over the course of 30 minutes. extraction effici ...200919627210
comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l.) fruits.simultaneous comparison of bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l) fruits for their anthocyanin composition, antimicrobial and antioxidant activity is reported. the aim of this study was to investigate and to compare anthocyanin composition, antimicrobial and antioxidant activity in bilberry and blueberry fruits and their skins. the investigations revealed that the highest amount of total anthocyanins was observed in fruits skins of blueberry cultivars. the resul ...200919702172
effects of ozone treatment on botrytis cinerea and sclerotinia sclerotiorum in relation to horticultural product quality.botrytis cinerea and sclerotinia sclerotiorum are fungal pathogens that cause the decay of many fruits and vegetables. ozone may be used as an antimicrobial agent to control the decay. the effect of gaseous ozone on spore viability of b. cinerea and mycelial growth of b. cinerea and s. sclerotiorum were investigated. spore viability of b. cinerea was reduced by over 99.5% (p < 0.01) and height of the aerial mycelium was reduced from 4.7 mm in the control to less than 1 mm after exposure to 450 o ...200919723209
degradation of anthocyanins and anthocyanidins in blueberry jams/stuffed fish.this study examined the effects of cooking on the degradation of anthocyanins and anthocyanidins of blueberries (vaccinium corymbosum l.) from cultivar bluecrop. fruits were used to prepare jams with different degrees brix and stuffed fish. a systematic evaluation of the degradation of anthocyanins and anthocyanidins of blueberries was performed; for that purpose an hplc/dad method was used to determine anthocyanin profile and anthocyanidin contents in fresh and cooked blueberries and in jams. t ...200919845348
host status of blueberry to invasive tephritid fruit flies in hawaii.forced infestation studies were conducted to determine whether northern or southern highbush blueberries, vaccinium corymbosum l., are hosts for the invasive tephritid fruit flies in hawaii. fruit were exposed to gravid female flies of bactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit fly), or bactrocera cucurbitae coquillet (melon fly) in screen cages outdoors for 6 h and then held on sand in the laboratory for 2 wk for pupal and adult emergenc ...200919886450
preparative separation of chlorogenic acid by centrifugal partition chromatography from highbush blueberry leaves (vaccinium corymbosum l.).blueberries (genus vaccinium) have gained worldwide focus because of the high anthocyanin content of their fruits. in contrast, the leaves of blueberry have not attracted any attention, even though they contain large quantities of chlorogenic acid, a strong antioxidant compound.201320310076
seasonal abundance, life history, and parasitism of caloptilia porphyretica (lepidoptera: gracillariidae), a leafminer of highbush blueberry.the leafminer caloptilia porphyretica braun (lepidoptera: gracillariidae), has become a frequent pest in commercial highbush blueberries, vaccinium corymbosum l., in new jersey, but little is known about its seasonal abundance, life history, and parasitism rates. monitoring programs were conducted from 2003 to 2006 on seven blueberry farms to determine the relative abundance of this leafminer by using pheromone-baited traps and by sampling vegetative and flower/fruit clusters and new shoot growt ...201020429440
bog bilberry (vaccinium uliginosum l.) extract reduces cultured hep-g2, caco-2, and 3t3-l1 cell viability, affects cell cycle progression, and has variable effects on membrane permeability.bog bilberry (vaccinium uliginosum l.) is a blue-pigmented edible berry related to bilberry (vaccinium myrtillus l.) and the common blueberry (vaccinium corymbosum). the objective of this study was to investigate the effect of a bog bilberry anthocyanin extract (bbae) on cell growth, membrane permeability, and cell cycle of 2 malignant cancer cell lines, caco-2 and hep-g2, and a nonmalignant murine 3t3-l1 cell line. bbae contained 3 identified anthocyanins. the most abundant anthocyanin was cyan ...201020492295
weather during bloom affects pollination and yield of highbush blueberry.weather plays an important role in spring-blooming fruit crops due to the combined effects on bee activity, flower opening, pollen germination, and fertilization. to determine the effects of weather on highbush blueberry, vaccinium corymbosum l., productivity, we monitored bee activity and compared fruit set, weight, and seed number in a field stocked with honey bees, apis mellifera l., and common eastern bumble bees, bombus impatiens (cresson). flowers were subjected to one of five treatments d ...201020568598
protective effect of enzymatic hydrolysates from highbush blueberry (vaccinium corymbosum l.) against hydrogen peroxide-induced oxidative damage in chinese hamster lung fibroblast cell line.blueberry was enzymatically hydrolyzed using selected commercial food grade carbohydrases (amg, celluclast, termamyl, ultraflo and viscozyme) and proteases (alcalase, flavourzyme, kojizyme, neutrase and protamex) to obtain water soluble compounds, and their protective effect was investigated against h(2)o(2)-induced damage in chinese hamster lung fibroblast cell line (v79-4) via various published methods. both amg and alcalase hydrolysates showed higher total phenolic content as well as higher c ...201020607062
cooked blueberries: anthocyanin and anthocyanidin degradation and their radical-scavenging activity.this study examined anthocyanin and anthocyanidin composition and radical-scavenging activity of three cultivars of blueberries (vaccinium corymbosum l., cv. bluecrop, bluetravel, and ozarkblue) before and after cooking. a total of 13 anthocyanins were separated and monitored in methanolic extracts of raw fruits by high-performance liquid chromatography/diode array detector (hplc/dad). principal component analysis using the anthocyanin profile as variables revealed differences according to culti ...201020662539
activity of broad-spectrum and reduced-risk insecticides on various life stages of cranberry fruitworm (lepidoptera: pyralidae) in highbush blueberry.laboratory and semifield bioassays were conducted to determine the life-stage activity of insecticides for controlling cranberry fruitworm, acrobasis vaccinii riley (lepidoptera: pyralidae), a key lepidopteran pest of highbush blueberry, vaccinium corymbosum l. the organophosphates azinphosmethyl and phosmet, the pyrethroid esfenvalerate, and the carbamate methomyl were lethal to all life stages. the neonicotinoids thiacloprid and acetamiprid demonstrated strong larvicidal and ovicidal activity ...201021061972
morphological and molecular characterization of phomopsis vaccinii and additional isolates of phomopsis from blueberry and cranberry in the eastern united states.forty isolates of phomopsis were obtained from twigs and berries of highbush blueberry, vaccinium corymbosum, and cranberry, vaccinium macrocarpon, isolated primarily from plants grown in the eastern united states. they were characterized using conidiomatal morphology, conidial dimensions, colony appearance and growth rate, and sequences of its rdna. based on morphological and molecular similarities, most isolates grouped together with an authentic culture of phomopsis vaccinii shear. this taxon ...200221156520
identification of aluminum-regulated genes by cdna-aflp analysis of roots in two contrasting genotypes of highbush blueberry (vaccinium corymbosum l.).to investigate the molecular mechanisms of al(3+)-stress in blueberry, a cdna-amplified fragment length polymorphism (cdna-aflp) analysis was employed to identify al-regulated genes in roots of contrasting genotypes of highbush blueberry (brigitta, al(3+)-resistant and bluegold, al(3+)-sensitive). plants grown in hydroponic culture were treated with 0 and 100 μm al(3+) and collected at different times over 48 h. seventy transcript-derived fragments (tdfs) were identified as being al(3+) responsi ...201121225377
[effects of exogenous nitric oxide on highbush blueberry psii photochemical activity and antioxidant system under high temperature stress].taking the test tube 'duke' highbush blueberry (vaccinium corymbosum) seedlings having been transplanted to the field for 6 months as test materials, this paper studied the effects of exogenous nitric oxide (no) on their growth, ps ii photochemical activity, and antioxidant system under high temperature stress. applying 0.2, 0.5, and 1.0 mmol x l(-1) of exogenous sodium nitroprusside (snp) could alleviate the decrease of maximum photochemical efficiency (fv/fm), actual photochemical efficiency u ...201021328939
edible neotropical blueberries: antioxidant and compositional fingerprint analysis.edible blueberry species are well recognized for their potential health benefits. ericaceae fruits including the north american highbush blueberry (vaccinium corymbosum l.) and five less common edible blueberry relatives from the new world tropics, anthopterus wardii ball, cavendishia grandifolia hoerold, macleania coccoloboides a. c. smith, sphyrospermum buxifolium poeppig & endlicher, and sphyrospermum cordifolium benth, were investigated for their antioxidant properties and phenolic profiles. ...201121391608
separation, characterization and quantification of phenolic compounds in blueberries and red and black currants by hplc-dad-esi-msn.the phenolic profile of four blueberry varieties (vaccinium corymbosum l., cv. toro, legacy, duke and bluecrop) and two varieties (rosenthal and rovada) of red currants (ribes rubrum l.) and black currants (ribes nigrum l.) cultivated in macedonia have been analyzed using hplc coupled to diode-array detection and tandem mass spectrometry with electrospray ionization. a complex profile of anthocyanins, flavonols, flavan-3-ols and hydroxycinnamic acid derivatives has been assayed in acetone-acetic ...201121401099
identification and field evaluation of attractants for the cranberry weevil, anthonomus musculus say.studies were conducted to develop an attractant for the cranberry weevil, anthonomus musculus, a pest of blueberry and cranberry flower buds and flowers in the northeastern united states. in previous studies, we showed that cinnamyl alcohol, the most abundant blueberry floral volatile, and the green leaf volatiles (z)-3-hexenyl acetate and hexyl acetate, emitted from both flowers and flower buds, elicit strong antennal responses from a. musculus. here, we found that cinnamyl alcohol did not incr ...201121445566
variation in highbush blueberry floral volatile profiles as a function of pollination status, cultivar, time of day and flower part: implications for flower visitation by bees.studies of the effects of pollination on floral scent and bee visitation remain rare, particularly in agricultural crops. to fill this gap, the hypothesis that bee visitation to flowers decreases after pollination through reduced floral volatile emissions in highbush blueberries, vaccinium corymbosum, was tested. other sources of variation in floral emissions and the role of floral volatiles in bee attraction were also examined.201121498566
susceptibility of low-chill blueberry cultivars to mediterranean fruit fly, oriental fruit fly, and melon fly (diptera: tephritidae).no-choice tests were conducted to determine whether fruit of southern highbush blueberry, vaccinium corymbosum l., hybrids are hosts for three invasive tephritid fruit flies in hawaii. fruit of various blueberry cultivars was exposed to gravid female flies of bactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit fly), or bactrocera cucurbitae coquillet (melon fly) in screen cages outdoors for 6 h and then held on sand in the laboratory for 2 wk for ...201121510205
classification of impacted blueberries during storage using an electronic nose.an electronic nose (en) was used to determine the effect of repeated impacts on changes in volatile characteristics of fresh blueberries during storage. hand-harvested 'misty' blueberries (vaccinium corymbosum) were treated either as (1) undropped (control) fruits, (2) fruits poured six times from a picking bucket 200 mm into a plastic field lug or (3) fruits dropped as above ten times onto a steel surface. fruits from all treatments were stored in vented polystyrene clamshell containers at 2 °c ...201121520446
phenolics in slovenian bilberries ( vaccinium myrtillus l.) and blueberries ( vaccinium corymbosum l.).phenolics from bilberries ( vaccinium myrtillus l.) sampled from seven different locations and highbush blueberries ( vaccinium corymbosum l.) from one location in slovenia were analyzed. in samples of both species 15 anthocyanins were identified by lc-ms/ms. their contents were expressed as cyanidin 3-glucoside equivalents (c3ge); bilberries contained 1210.3 ± 111.5 mg c3ge/100 g fw and blueberries 212.4 ± 14.1 mg c3ge/100 g fw. glycosides of delphinidin and cyanidin were predominant (488.5 vs ...201121574578
biochemical and molecular changes in response to aluminium-stress in highbush blueberry (vaccinium corymbosum l.).aluminium (al) stress is an important factor limiting crop yields in acid soils. despite this, very little is known about the mechanisms of resistance to this stress in woody plants. to understand the mechanisms of al-toxicity and response in blueberries, we compared the impact of al-stress in al-resistant and al-sensitive genotypes using vaccinium corymbosum l. (ericaceae) as a plant model. we investigated the effect of al-stress on the physiological performance, oxidative metabolism and expres ...201121596576
root anatomy, morphology, and longevity among root orders in vaccinium corymbosum (ericaceae).understanding root processes at the whole-plant or ecosystem scales requires an accounting of the range of functions within a root system. studying root traits based on their branching order can be a powerful approach to understanding this complex system. the current study examined the highly branched root system of the ericoid plant, vaccinium corymbosum l. (highbush blueberry) by classifying its root orders with a modified version of the morphometric approach similar to that used in hydrology ...200821628158
volatile composition of four southern highbush blueberry cultivars and effect of growing location and harvest date.the volatile composition of four southern highbush blueberry cultivars ('primadonna', 'jewel', 'snowchaser', and 'fl02-40') grown in two locations (gainesville and haines city, fl) and harvested multiple times was investigated. a total of 42 volatiles were identified, including 8 esters, 12 terpenoids, 11 aldehydes, 7 alcohols, and 4 ketones. twelve of these volatiles are reported for the first time in highbush blueberries, with 10 being positively identified: (z)-3-hexenal, (e,e)-2,4-hexadienal ...201121721574
an efficient rna isolation procedure and identification of reference genes for normalization of gene expression in blueberry.application of transcriptomics approaches can greatly enhance our understanding of blueberry physiology. the success of transcriptomics approaches is dependent on the extraction of high-quality rna which is complicated by the abundance of polyphenolics and polysaccharides in blueberry. additionally, transcriptomics requires the accurate quantification of transcript abundance. quantitative real-time polymerase chain reaction (qrt-pcr) is a robust method to determine transcript abundance. normaliz ...201121761237
cultivar evaluation and effect of fermentation on antioxidant capacity and in vitro inhibition of α-amylase and α-glucosidase by highbush blueberry (vaccinium corombosum).the berry fruits of highbush blueberry (vaccinium corymbosum) contain bioactive compounds with potential health benefits. the objective was to evaluate blueberries grown in southern illinois as well as the effect of fermentation, at two different temperatures, on chemical and physical parameters. fruits from fifteen blueberry cultivars were analyzed. fruit diameter ranged from 12.8 mm to 18.7 mm, ph from 2.6 to 3.7, reducing sugars from 6.4% to 15.2%, total sugars from 13.9% to 21.6%, total poly ...201121761898
complete genome sequences of blueberry red ringspot virus (caulimoviridae) isolates from the czech republic and slovenia.genomic dna of blueberry red ringspot virus (genus soymovirus, family caulimoviridae) from highbush blueberry plants growing for years in the czech republic and slovenia was sequenced. the circular dsdna genomes consist of 8,303 and 8,299 nt, respectively, and contain eight open reading frames (orfs) longer than 100 amino acids. the european isolates are 90% to 99% identical in aa sequences of distinct proteins. in contrast to the american new jersey isolate, in-frame initiation codons have been ...201121796400
Blueberry intake alters skeletal muscle and adipose tissue peroxisome proliferator-activated receptor activity and reduces insulin resistance in obese rats.Abstract Metabolic syndrome can precede the development of type 2 diabetes and cardiovascular disease and includes phenotypes such as obesity, systemic inflammation, insulin resistance, and hyperlipidemia. A recent epidemiological study indicated that blueberry intake reduced cardiovascular mortality in humans, but the possible genetic mechanisms of this effect are unknown. Blueberries are a rich source of anthocyanins, and anthocyanins can alter the activity of peroxisome proliferator-activat ...201121861718
gene expression and metabolite profiling of developing highbush blueberry fruit indicates transcriptional regulation of flavonoid metabolism and activation of abscisic acid metabolism.highbush blueberry (vaccinium corymbosum) fruits contain substantial quantities of flavonoids, which are implicated in a wide range of health benefits. although the flavonoid constituents of ripe blueberries are known, the molecular genetics underlying their biosynthesis, localization, and changes that occur during development have not been investigated. two expressed sequence tag libraries from ripening blueberry fruit were constructed as a resource for gene identification and quantitative real ...201222086422
procyanidin, anthocyanin, and chlorogenic acid contents of highbush and lowbush blueberries.the health benefits of blueberry consumption on the vascular system and brain are mediated in part by their flavonoid content. in light of this, six cultivated highbush blueberry varieties ( vaccinium corymbosum l.) and one lowbush or wild blueberry ( vaccinium angustifolium l.) were analyzed for their anthocyanin, flavanol oligomer, and chlorogenic acid contents. the highbush varieties bluecrop, o'neal, bluejay, and brigitta had significantly greater levels of anthocyanidins compared to the oth ...201222175691
Ptcorp gene induced by cold stress was identified by proteomic analysis in leaves of Poncirus trifoliata (L.) Raf.A proteomic approach was employed to investigate the cold stress-responsive proteins in trifoliate orange (Poncirus trifoliata (L.) Raf.), which is a well-known cold tolerant citrus relative and widely used as rootstock in China. Two-year-old potted seedlings were exposed to freezing temperature (-6°C) for 50 min (nonlethal) and 80 min (lethal), and the total proteins were isolated from leaves of the treated plants. Nine differentially accumulated proteins over 2-fold changes in abundance were i ...201122205537
influence of native flowering plant strips on natural enemies and herbivores in adjacent blueberry fields.conservation plantings of native wildflowers were established adjacent to highbush blueberry (vaccinium corymbosum l.) fields to test the hypothesis that provision of resources for natural enemies increases their abundance in adjacent crop fields without increasing the abundance of pest insects. for two growing seasons, natural enemies and herbivorous insects were sampled in fields with flowering borders and in control fields where growers maintained standard mown grass perimeters. insects were ...201122251649
examining the spatial distribution of flower thrips in southern highbush blueberries by utilizing geostatistical methods.flower thrips (frankliniella spp.) are one of the key pests of southern highbush blueberries (vaccinium corymbosum l. x v. darrowii camp), a high-value crop in florida. thrips' feeding and oviposition injury to flowers can result in fruit scarring that renders the fruit unmarketable. flower thrips often form areas of high population, termed "hot spots", in blueberry plantings. the objective of this study was to model thrips spatial distribution patterns with geostatistical techniques. semivariog ...201122251691
high performance liquid chromatography analysis of anthocyanins in bilberries (vaccinium myrtillus l.), blueberries (vaccinium corymbosum l.), and corresponding juices.in the present study the anthocyanin content of commercially available bilberry juices and fresh fruits were quantified by using 15 authentic anthocyanin standards via high performance liquid chromatography with an ultra-violet detector (hplc-uv/vis). delphinidin-3-o-glucopyranoside, delphinidin-3-o-galactopyranoside, and cyanidin-3-o-arabinopyranoside were the major anthocyanins found in juices, nectar, and fresh bilberries. in contrast, fresh blueberries had higher concentrations of malvidin-3 ...201222394068
generation and analysis of blueberry transcriptome sequences from leaves, developing fruit, and flower buds from cold acclimation through deacclimation.there has been increased consumption of blueberries in recent years fueled in part because of their many recognized health benefits. blueberry fruit is very high in anthocyanins, which have been linked to improved night vision, prevention of macular degeneration, anti-cancer activity, and reduced risk of heart disease. very few genomic resources have been available for blueberry, however. further development of genomic resources like expressed sequence tags (ests), molecular markers, and genetic ...201222471859
emergence of blueberry maggot flies (diptera: tephritidae) from mulches and soil at various depths.control of blueberry maggot, rhagoletis mendax curran, typically is achieved with insecticides targeting adult flies before females oviposit in ripening fruit. management strategies targeting other life stages have received less attention. we tested effects of compost or pine needle mulches on emergence of blueberry maggot flies under laboratory and field conditions. few flies emerged from pupae that were buried under 20 cm of pine needles in all experiments, but burial in 20 cm of compost did n ...201222507011
effects of southern highbush blueberry cultivar and treatment threshold on flower thrips populations.in florida, southern highbush (shb) blueberries (vaccinium corymbosum l. x vaccinium darrowi camp) are grown for a highly profitable early season fresh market. flower thrips are the key pest of shb blueberries, and frankliniella bispinosa (morgan) is the most common species found. flower thrips injure blueberry flowers by feeding and ovipositing in all developing tissues. these injuries can lead to scarring of developing fruit. the objectives of this study were to determine the relationship betw ...201222606818
comparative effects of nitrogen fertigation and granular fertilizer application on growth and availability of soil nitrogen during establishment of highbush blueberry.a 2-year study was done to compare the effects of nitrogen (n) fertigation and granular fertilizer application on growth and availability of soil n during establishment of highbush blueberry (vaccinium corymbosum l. "bluecrop"). treatments included four methods of n application (weekly fertigation, split fertigation, and two non-fertigated controls) and four levels of n fertilizer (0, 50, 100, and 150 kg·ha(-1) n). fertigation treatments were irrigated by drip and injected with a liquid urea sol ...201122639596
distribution and phenology of dasineura oxycoccana (diptera: cecidomyiidae) in michigan blueberries.the blueberry gall midge, dasineura oxycoccana johnson, is a serious pest of rabbiteye blueberries in florida, georgia, and mississippi, and a potential pest of southern and northern highbush blueberries. its damage has been observed with increasing frequency in highbush blueberry plantings in the great lakes region, including in wisconsin and in michigan. unlike in rabbiteye blueberry plantings, where blueberry gall midge primarily damages flowering buds, it is found to damage only the vegetati ...201222732602
an improved method for rna isolation from plants using commercial extraction kits.isolation of rna from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. this study presents a simple and efficient modification of rna extraction from different ...201222826824
blanching improves anthocyanin absorption from highbush blueberry ( vaccinium corymbosum l.) purée in healthy human volunteers: a pilot study.blueberries ( vaccinium corymbosum l.) are rich sources of phenolics and anthocyanins (acns). we investigated the absorption of acns after consumption of one portion (300 g) of minimally processed blueberry purée (p) obtained from blanched (bl) or unblanched (nb) berries. a repeated-measures, crossover design study was conducted on healthy human volunteers. blood was drawn between baseline and 24 h after bl-p or nb-p consumption, while urine were collected from the day before the experiment up t ...201222906096
phenology of dasineura oxycoccana (diptera: cecidomyiidae) on cranberry and blueberry indicates potential for gene flow.dasineura oxycoccana (johnson) (diptera: cecidomyiidae) is a pest of cranberry, vaccinium macrocarpon (aiton) (ericales: ericaceae), and highbush blueberry, vaccinium corymbosum (l.) (ericales: ericaceae), in north america. in british columbia, canada, d. oxycoccana was first found on highbush blueberry in 1991 and then on cranberry seven years later. because many cranberry and highbush blueberry farms are adjacent to one another, we hypothesized that d. oxycoccana was moving from highbush blueb ...201222928299
antioxidant and α-glucosidase inhibitory phenolics isolated from highbush blueberry flowers.blueberries have been extensively researched, but there are limited studies on other parts of the plant. here we report the first phytochemical examination of highbush blueberry (vaccinium corymbosum) flowers, which yielded 21 phenolics. the compounds were identified from extensive nmr and mass spectral analyses and included five caffeic acid (1-5), three coumaric acid (6-8), and two cinnamyl alcohol (9-10) derivatives, eight flavonol glycosides (11-18), and three phenylpropanoid-substituted cat ...201222953942
ground and rove beetles (coleoptera: carabidae and staphylinidae) are affected by mulches and weeds in highbush blueberries.biological control of insects by predators may be indirectly influenced by management practices that change the invertebrate community in agroecosystems. in this study we examined effects that mulching and weeding have on predatory beetles (carabidae and staphylinidae) and their potential prey in a highbush blueberry field. we compared beetle communities in unweeded control plots to those that were weeded and/or received a single application of compost or pine needle mulch. compost mulch and wee ...201223068165
intraspecies differences in cold hardiness, carbohydrate content and β-amylase gene expression of vaccinium corymbosum during cold acclimation and deacclimation.changes in cold hardiness, carbohydrate content and β-amylase gene expression were monitored in the shoots of the highbush blueberry (vaccinium corymbosum l.) cultivars 'sharpblue' and 'jersey' during cold acclimation (ca) and deacclimation (da). the seasonal patterns were similar in both cultivars, but the levels of cold hardiness determined by electrolyte leakage analysis were significantly different; 'jersey' was hardier than 'sharpblue'. cold hardiness was closely related to total soluble su ...201223135736
rainfastness of insecticides used to control japanese beetle in blueberries.field-based bioassays were used to determine the relative impact of rainfall on the relative toxicity of four insecticides, phosmet, carbaryl, zeta-cypermethrin, or imidacloprid, from different chemical classes on adult japanese beetles, popillia japonica newman, in highbush blueberries, vaccinium corymbosum l. bioassays were set up 24 h after spraying occurred and japanese beetle condition was scored as alive, knockdown or immobile 1, 24, and 48 h after bioassay setup. all insecticides were sig ...201223156165
micropropagation of vaccinium sp. by in vitro axillary shoot proliferation.the vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (vaccinium × corymbosum l.), cranberry (vaccinium macrocarpon ait.), and lingonberry (vaccinium vitis-idaea l.). in some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. the demand for nursery plants could be fulfilled only by micropropagation. in principle cultivars are propagated in vitro through similar t ...201323179690
populations of the gall midge dasineura oxycoccana on cranberry and blueberry produce and respond to different sex pheromones.we identified and field-tested the sex pheromones of dasineura oxycoccana (johnson) (diptera: cecidomyiidae) midges collected from cranberry, vaccinium macrocarpon aiton, and from highbush blueberry, vaccinium corymbosum l., commonly named cranberry tipworm (ctw) and blueberry gall midge (bgm), respectively. coupled gas chromatographic-electroantennographic detection (gc-ead) analyses of pheromone gland extract from the ovipositor of calling ctw females revealed one component (<10 pg per oviposi ...201323297107
relationship between polyphenol content and anti-influenza viral effects of berries.berries are known to have many kinds of biological activities. we focused on their antiviral effect, which has not yet been well evaluated.201323355221
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