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two new species in the pichia guilliermondii clade: pichia caribbica sp. nov., the ascosporic state of candida fermentati, and candida carpophila comb. nov.pichia caribbica sp. nov. (type strain dbvpg 4519, nrrl y-27274, cbs 9966) is described as the ascosporic state of candida fermentati, and candida guilliermondii var. carpophila (type strain dbvpg 7739, nrrl y-17905, cbs 5256) is elevated to species status as candida carpophila comb. nov. these new taxa, which are indistinguishable on the basis of conventional taxonomic criteria, differ from one another and from pichia guilliermondii by low dna base sequence relatedness, different electrophoreti ...200515691751
bioconversion of ferulic acid to 4-vinylguaiacol and 4-ethylguaiacol and of 4-vinylguaiacol to 4-ethylguaiacol by halotolerant yeasts belonging to the genus candida.in order to examine the genesis of the characteristic flavors of soy sauce and miso, seven novel halotolerant yeast strains of two types, which showed convertibility of ferulic acid (fa) to 4-vinylguaiacol (4-vg) and to 4-ethylguaiacol (4-eg), were isolated from miso-koji and miso pastes. two of these strains were identified as candida guilliermondii (anamorph of pichia guilliermondii), and candida fermentati (anamorph of pichia caribbica), based on sequence analyses of a partial 26s ribosomal r ...200717420598
first isolation of microorganisms from the gut diverticulum of aedes aegypti (diptera: culicidae): new perspectives for an insect-bacteria association.we show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. the gut diverticulum from newly emerged and non-fed aedes aegypti was dissected under aseptic conditions, homogenized and plated on bhi medium. microbial isolates were identified by sequencing of 16s rdna for bacteria and 28s rdna for yeast. a direct dna extraction from ae. aegypti gut diverticulum was also performed. the bacterial isolates were: bacillus sp., ba ...200718209929
isolation and characterization of xylitol-producing yeasts from the gut of colleopteran insects.a total of 35 yeasts were isolated from the gut of beetles collected from hyderabad city, india. twenty of these yeasts utilized xylose as a sole carbon source but only 12 of these converted xylose to xylitol. the ability to convert xylose to xylitol varied among the isolates and ranged from 0.12 to 0.58 g/g xylose. based on the phenotypic characteristics and phylogenetic analysis of the d1/d2 domain sequence of 26s rrna gene, these isolates were identified as members of pichia, candida, issatch ...200717710483
yeast species involved in artisanal cachaça fermentation in three stills with different technological levels in pernambuco, brazil.both the taste and aroma of cachaça, an alcoholic beverage produced by yeast fermentation of sugar cane, are influenced by yeast metabolites and volatiles. the knowledge of yeast population dynamics during the fermentation process will help to establish the basis for quality control of alcoholic beverage. in the present work, the population dynamics of three fermentation processes, with differing levels of technological sophistication, were studied. saccharomyces cerevisiae was found to be the d ...200919465241
yeast diversity of ghanaian cocoa bean heap fermentations.the fermentation of the theobroma cacao beans, involving yeasts, lactic acid bacteria, and acetic acid bacteria, has a major influence on the quality of the resulting cocoa. an assessment of the microbial community of cocoa bean heap fermentations in ghana resulted in 91 yeast isolates. these were grouped by pcr-fingerprinting with the primer m13. representative isolates were identified using the d1/d2 region of the large subunit rrna gene, internal transcribed spacer sequences and partial actin ...200919473277
yeast biodiversity from oleic ecosystems: study of their biotechnological properties.the aim of this study was to know the yeast biodiversity from fresh olive (olea europaea l.) fruits, olive paste (crush olives) and olive pomace (solid waste) from arbequina and cornicabra varieties. yeasts were isolated from fruits randomly harvested at various olive groves in the region of castilla la mancha (spain). olive paste and pomace, a byproduct of the processing of this raw material, were also collected in sterile flasks from different oil mills. molecular identification methodology us ...201020417397
efficient chemical and enzymatic saccharification of the lignocellulosic residue from agave tequilana bagasse to produce ethanol by pichia caribbica.bagasse of agave tequilana (bat) is the residual lignocellulosic waste that remains from tequila production. in this study we characterized the chemical composition of bat, which was further saccharified and fermented to produce ethanol. bat was constituted by cellulose (42%), hemicellulose (20%), lignin (15%), and other (23%). saccharification of bat was carried out at 147°c with 2% sulfuric acid for 15 min, yielding 25.8 g/l of fermentable sugars, corresponding to 36.1% of saccharificable mate ...201021072557
identification of yeasts during alcoholic fermentation of tchapalo, a traditional sorghum beer from côte d'ivoire.this study investigated the diversity and dynamics of yeasts involved in alcoholic fermentation of a traditional sorghum beer from côte d'ivoire, tchapalo. a total of 240 yeast strains were isolated from fermenting sorghum wort inoculated with dry yeast from two geographic regions of côte d'ivoire (abidjan and bondoukou). initial molecular identification to the species level was carried out using rflp of pcr-amplified internal transcribed spacers of rdna (its1-5.8s-its2). ten different profiles ...201121318423
assessment of the yeast species composition of cocoa bean fermentations in different cocoa-producing regions using denaturing gradient gel electrophoresis.the yeast species composition of 12 cocoa bean fermentations carried out in brazil, ecuador, ivory coast and malaysia was investigated culture-independently. denaturing gradient gel electrophoresis of 26s rrna gene fragments, obtained through polymerase chain reaction with universal eukaryotic primers, was carried out with two different commercial apparatus (the dcode and cbs systems). in general, this molecular method allowed a rapid monitoring of the yeast species prevailing during fermentatio ...201122093683
enhancement of biocontrol efficacy of pichia carribbica to postharvest diseases of strawberries by addition of trehalose to the growth medium.the effects of trehalose on the antagonistic activity of pichia caribbica against rhizopus decay and gray mold decay of strawberries and the possible mechanisms involved were investigated. the proteomic analysis and comparison of p. carribbica in response to trehalose was analyzed based on two-dimensional gel electrophoresis. the antagonistic activity of p. carribbica harvested from the culture media of nydb amended with trehalose at 0.5% was improved greatly compared with that without trehalose ...201222489189
evaluation of a potential starter culture for enhance quality of coffee fermentation.the coffee fermentation is characterized by the presence of different microorganisms belonging to the groups of bacteria, fungi and yeast. the objectives of this work were to select pectinolytic microorganisms isolated from coffee fermentations and evaluate their performance on coffee pulp culture medium. the yeasts and bacteria isolates were evaluated for their activity of polygalacturonase (pg), pectin lyase (pl) and pectin methylesterase (pme) and metabolites production. among 127 yeasts isol ...201323054699
effect of trehalose on the biocontrol efficacy of pichia caribbica against post-harvest grey mould and blue mould decay of apples.the influence of adding trehalose to the culture medium on the efficacy of pichia caribbica (jsu-1) in controlling post-harvest grey mould and blue mould decay of apples and the possible mechanisms involved were investigated.201323325746
identification and antifungal susceptibility of a large collection of yeast strains isolated in tunisian hospitals.in this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was used as a rapid method to identify yeasts isolated from patients in tunisian hospitals. when identification could not be exstablished with this procedure, sequencing of the internal transcribed spacer with 5.8s ribosomal dna (rdna) (its1-5.8s-its2) and d1/d2 domain of large-subunit (lsu rdna) were employed as a molecular approach for species differentiation. candida albicans was the dom ...201323768242
crystalline xylitol production by a novel yeast, pichia caribbica (hq222812), and its application for quorum sensing inhibition in gram-negative marker strain chromobacterium violaceum cv026.xylitol, a sugar alcohol, is fast gaining ground over other artificial sugar substitutes owing to its advantageous properties. xylitol is a safer alternative for diabetics because of insulin-independent metabolism. it has beneficial properties suitable to form an important part of odontological formulations. conventional commercial production of xylitol involves harsh chemical method operating at high temperature and pressure. thus, microbial production of xylitol is preferred over chemical meth ...201323338824
biocontrol of postharvest rhizopus decay of peaches with pichia caribbica.a new yeast antagonist, pichia caribbica, isolated in our laboratory from the soil collected from unsprayed orchards, was evaluated for its biocontrol capability against rhizopus stolonifer on peaches and the possible mechanisms involved. the decay incidence and lesion diameter of rhizopus decay of peaches treated by p. caribbica were significantly reduced compared with the control fruits, and the higher the concentration of p. caribbica, the better the efficacy of the biocontrol. rapid coloniza ...201323536217
efficacy of pichia caribbica in controlling blue mold rot and patulin degradation in apples.the efficacy of pichia caribbica in controlling postharvest blue mold and natural decay development of apples and degrading the patulin produced by penicillium expansum was investigated. the decay incidence of the blue mold of apples treated by p. caribbica was significantly reduced compared with the control samples, and the higher the concentration of p. caribbica, the better the efficacy of the biocontrol. p. caribbica significantly controlled the natural decay development of apples following ...201323416552
the effects of co-culturing non-saccharomyces yeasts with s. cerevisiae on the sugar cane spirit (cachaça) fermentation process.twenty non-saccharomyces strains were previously tested in pure culture for their ability to grow in 12 % ethanol, their β-glucosidase activity, flocculation, glycerol, ethanol and acetic acid production, fermentation kinetics and their production of volatile compounds. of these 20 strains, three strains, namely, pichia anomala ufla caf70, p. anomala ufla caf119 and pichia caribbica ufla caf733, were evaluated in co-culture with saccharomyces cerevisiae ufla ca11. of the mixed inocula, the mixtu ...201322911390
interactions between a sap beetle, sabal palm, scale insect, filamentous fungi and yeast, with discovery of potential antifungal compounds.the multi-trophic relationship between insects, yeast, and filamentous fungi is reported on sabal palm (sabal palmetto (walter) lodd. ex schult. & schult. f.). gut content analyses and observations of adult and larval feeding of the sap beetle brachypeplus glaber leconte indicate that niche partitioning of fungal food substrata occurs between adults and larvae. this is the first report of specific mycophagous niche partitioning among beetle life stages based on gut content analyses. fungi isolat ...201424586669
ascorbic acid enhances oxidative stress tolerance and biological control efficacy of pichia caribbica against postharvest blue mold decay of apples.the effect of ascorbic acid (vc) on improving oxidative stress tolerance of pichia caribbica and biocontrol efficacy against blue mold caused by penicillium expansum on apples was investigated. p. caribbica showed susceptibility to the oxidative stress in vitro test, and 250 μg/ml vc treatment improved its oxidative stress tolerance. the higher viability exhibited by vc-treated yeast was associated with a lower intracellular ros level. the activities of antioxidant enzymes of p. caribbica were i ...201425029482
reliable differentiation of meyerozyma guilliermondii from meyerozyma caribbica by internal transcribed spacer restriction fingerprinting.meyerozyma guilliermondii (anamorph candida guilliermondii) and meyerozyma caribbica (anamorph candida fermentati) are closely related species of the genetically heterogenous m. guilliermondii complex. conventional phenotypic methods frequently misidentify the species within this complex and also with other species of the saccharomycotina ctg clade. even the long-established sequencing of large subunit (lsu) rrna gene remains ambiguous. we also faced similar problem during identification of yeas ...201424575831
diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid.yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ...201424442819
isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process.during wet processing of coffee, the ripe cherries are pulped, then fermented and dried. this study reports an experimental approach for target identification and selection of indigenous coffee yeasts and their potential use as starter cultures during the fermentation step of wet processing. a total of 144 yeast isolates originating from spontaneously fermenting coffee beans were identified by molecular approaches and screened for their capacity to grow under coffee-associated stress conditions. ...201425087206
isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source.few yeasts have shown the potential to efficiently utilize hemicellulosic hydrolyzate as the carbon source. in this study, microorganisms isolated from the manaus region in amazonas, brazil, were characterized based on their utilization of the pentoses, xylose, and arabinose. the yeasts that showed a potential to assimilate these sugars were selected for the better utilization of lignocellulosic biomass. two hundred and thirty seven colonies of unicellular microorganisms grown on hemicellulosic ...201526436401
genetic diversity of medically important and emerging candida species causing invasive infection.genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis.201525887032
microbiological diversity associated with the spontaneous wet method of coffee fermentation.the evaluation of the microbiota present during coffee wet fermentation was done in two distinct regions of minas gerais, brazil: one farm in the south of minas gerais (lavras=l) and another farm in the savannah region (monte carmelo=mc). the yeast population ranged from 2.48 to 4.92 log cfu/g and from 2 to 4.81 log cfu/g, the mesophilic bacteria population ranged from 3.83 to 8.47 log cfu/g and from 5.37 to 7.36 log cfu/g, and the lab population ranged from 2.57 to 5.66 log cfu/g and from 3.40 ...201526119187
characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol.this study attempted to identify oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glycerol. two-step screening of 387 yeast strains revealed 23 oleaginous strains that accumulated quantities of lipid higher than 20 % of their biomass when cultivated in glycerol. these strains were identified to be four ascomycetous yeast species i.e. candida silvae, kodamaea ohmeri, meyerozyma caribbica, and pichia manshurica, and five basidiomyce ...201526615742
complete genome sequence of halophilic yeast meyerozyma caribbica mg20w isolated from rhizosphere soil.meyerozyma caribbica mg20w was originally isolated from rhizosphere soil on reclaimed land in the republic of korea. we describe herein the 10.64-mbp-long genome sequence of m. caribbica mg20w, which exhibits high salt resistance.201525792051
development of an affordable typing method for meyerozyma guilliermondii using microsatellite markers.despite previously published methods, there is still a lack of rapid and affordable methods for genotyping the meyerozyma guilliermondii yeast species. the development of microsatellite markers is a useful genotyping method in several yeast species. using the tandem repeat finder software, a total of 19 microsatellite motifs (di-, tri-, and tetra- repetition) were found in silico in seven of the nine scaffolds published so far. primer pairs were designed for all of them, although only four were ...201626476570
identification of yeasts isolated from raffia wine (raphia hookeri) produced in côte d'ivoire and genotyping of saccharomyces cerevisiae strains by pcr inter-delta.raffia wine is a traditional alcoholic beverage produced in several african countries where it plays a significant role in traditional customs and population diet. alcoholic fermentation of this beverage is ensured by a complex natural yeast flora which plays a decisive role in the quality of the final product. this present study aims to evaluate the distribution and the diversity of the yeast strains isolated in raffia wine from four sampling areas (abengourou, alépé, grand-lahou and adzopé) in ...201627339306
the possible mechanisms involved in degradation of patulin by pichia caribbica.in this work, we examined the mechanisms involved in the degradation of patulin by pichia caribbica. our results indicate that cell-free filtrate of p. caribbica reduced patutlin content. the heat-killed cells could not degrade patulin. however, the live cells significantly reduced the concentration of the patulin. in furtherance to this, it was observed that patulin was not detected in the broken yeast cells and cell wall. the addition of cycloheximide to the p. caribbica cells decreased the ca ...201627735830
bacaba beverage produced by umutina brazilian amerindians: microbiological and chemical characterization.bacaba chicha is a beverage prepared by the indigenous umutina people from the bacaba fruit (oenocarpus bacaba), a purple berry that is rich in fat and carbohydrates, as well as a source of phenolic compounds. in this study, samples of bacaba chicha beverage were collected, and the microbial community was assessed using culture-dependent and -independent techniques. the nutritional composition and metabolite profiles were analyzed, and species belonging to lactic acid bacteria (lab) and yeasts w ...201726691483
bioprospection of cold-adapted yeasts with biotechnological potential from antarctica.the aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de mayo island, antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. a total of 105 yeast isolates were obtained from different samples and 66 were identified. they belonged to 12 basidiomycetous and four ascomycetous genera. most of the isolates were ascribed to the genera cryptococcus, mrakia, cystobasidium, rh ...201728272809
purification and characterization of xylitol dehydrogenase with l-arabitol dehydrogenase activity from the newly isolated pentose-fermenting yeast meyerozyma caribbica 5xy2.meyerozyma caribbica strain 5xy2, which was isolated from an alcohol fermentation starter in thailand, was found to catabolize l-arabinose as well as d-glucose and d-xylose. the highest production amounts of ethanol from d-glucose, xylitol from d-xylose, and l-arabitol from l-arabinose were 0.45 g/g d-glucose, 0.60 g/g d-xylose, and 0.61 g/g l-arabinose with 21.7 g/l ethanol, 20.2 g/l xylitol, and 30.3 g/l l-arabitol, respectively. the enzyme with l-arabitol dehydrogenase (lad) activity was puri ...201727506274
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