| enhancement of fumaric acid production by rhizopus oryzae using a two-stage dissolved oxygen control strategy. | batch fermentative production of fumaric acid by rhizopus oryzae me-f12 was investigated in a 7-l stirred tank fermentor under different dissolved oxygen (do) concentrations. high fumaric acid yield on glucose (0.56 g/g) was achieved under high do concentration (80%), but the glucose consumption rate and fumaric acid productivity were rather low (0.91 and 0.51 g/l/h). fumaric acid productivity was enhanced under low do concentration (30%), but the fuamric acid yield on glucose decreased to 0.52 ... | 2010 | 19936636 |
| [stability of whole cell biocatalyst for biodiesel production from renewable oils]. | lipase-mediated biodiesel production becomes increasingly important because of mild reaction conditions, pollution free during the process and easy product separation. compared with traditional immobilized lipase, whole cell biocatalyst is promising for biodiesel production because it is easy to prepare and has higher enzyme activity recovery. rhizopus oryzae ifo4697 can be used as the catalyst for biodiesel production. to further study the stability of the whole cell biocatalyst is extremely im ... | 2009 | 19938482 |
| significant changes in the transesterification activity of free and mesoporous-immobilized rhizopus oryzae lipase in ionic liquids. | we examined the activity of free rhizopus oryzae lipase (rol) and rol immobilized on mesoporous materials in transesterification reactions in various dialkylimidazolium-cation based ionic liquids. for free rol, the highest activity (0.39 u/mg protein) was obtained in [omim][pf(6)] followed by that (0.28 u/mg protein) in [bmim][pf(6)]. specific activities of rol immobilized on mesocellular foam (mcf) were only 0.47 and 0.43 u/mg protein in [omim][pf(6)] and [bmim][pf(6)], respectively. however, t ... | 2010 | 19948194 |
| sterols isolated from nuruk (rhizopus oryzae ksd-815) inhibit the migration of cancer cells. | an activity-guided fractionation method was used to isolate anticancer components from nuruk (rhizopus oryzae ksd-815:ksd-815). dried powder of ksd-815 was extracted with 80% methanol and partitioned successively using nhexane, ethyl acetate, n-butanol, and water. the n-hexane and n-butanol fractions showed a strong antimigratory effect on human cancer cells. both of these fractions were subjected to separation and purification procedures using silica gel, octadecyl silica gel, and sephadex lh-2 ... | 2009 | 19996683 |
| production of lactic acid from raw sweet potato powders by rhizopus oryzae immobilized in sodium alginate capsules. | rhizopus oryzae immobilized in calcium alginate was applied in lactic acid fermentation with unhydrolyzed raw sweet potato powders as the sole carbon source. the effects of sodium alginate concentration, calcium chloride concentration, and the immobilized bead diameter on lactic acid production were investigated. increase in sodium alginate concentration during the gelation process would harden the immobilized capsule, which led to a decrease in lactic acid production. the increase in calcium ch ... | 2010 | 20020223 |
| comparative pharmacodynamics of amphotericin b lipid complex and liposomal amphotericin b in a murine model of pulmonary mucormycosis. | we compared the kinetics of amphotericin b (amb) lung accumulation and fungal clearance by liposomal amphotericin b (l-amb) and amphotericin b lipid complex (ablc) in a neutropenic murine model of invasive pulmonary mucormycosis (ipm). immunosuppressed balb/c mice were inoculated with 1 x 10(6) rhizopus oryzae spores and administered l-amb or ablc at daily intravenous doses of 1, 5, or 10 mg/kg of body weight for 5 days starting 12 h after infection. at a dose of 10 mg/kg/day, both l-amb and abl ... | 2010 | 20038620 |
| optimization of lactic acid production by pellet-form rhizopus oryzae in 3-l airlift bioreactor using response surface methodology. | the influence of two key environmental factors, ph and oxygen transfer coefficient (k(l)a), was evaluated on the lactic acid production as the main answer and, on the size of cell pellets of the fungal strain rhizopus oryzae kps106, as second dependant answer by response surface methodology using a central composite design. the results of the analysis of variance and modeling demonstrated that ph and k(l)a had a significant effect on lactic acid production by this strain. however, no interaction ... | 2010 | 20091139 |
| cunninghamella bertholletiae exhibits increased resistance to human neutrophils with or without antifungal agents as compared to rhizopus spp. | among zygomycetes, cunninghamella bertholletiae occurs less frequently as the etiologic agent of human disease but causes more aggressive, refractory, and fatal infections despite antifungal therapy. little is known about the differential innate host response against cunninghamella and other zygomycetes in the presence of antifungal agents. we therefore studied the activity of human neutrophils (pmns) alone or in combination with caspofungin, posaconazole (psc), and voriconazole (vrc) against hy ... | 2010 | 20100138 |
| effects of tertiary treatment by fungi on organic compounds in a kraft pulp mill effluent. | pulp and paper mills generate a plethora of pollutants depending upon the type of pulping process. efforts to mitigate the environmental impact of such effluents have been made by developing more effective biological treatment systems in terms of biochemical oxygen demand, chemical oxygen demand, colour and lignin content. this study is the first that reports an evaluation of the effects of a tertiary treatment by fungi (pleurotus sajor caju, trametes versicolor and phanerochaete chrysosporium a ... | 2010 | 20101467 |
| in vitro interaction of posaconazole with calcineurin inhibitors and sirolimus against zygomycetes. | zygomycosis is an uncommon but devastating disease with few therapeutic options. calcineurin inhibitors and sirolimus (mtor inhibitor), commonly used in transplant patients as immunosuppressives, have antifungal activity. they are known to demonstrate synergy with triazoles against certain fungi, though limited data exist about their activity against zygomycetes. our aim was to study the in vitro interaction of posaconazole with calcineurin inhibitors and sirolimus against zygomycetes. | 2010 | 20130026 |
| utilization of protein extract from dairy manure as a nitrogen source by rhizopus oryzae nrrl-395 for l-lactic acid production. | six levels of crude protein (0.21, 0.42, 0.84, 1.68, 2.52, and 3.36g/l) and six levels of protein hydrolysates from dairy manure, defined by degree of hydrolysis (dh, 6.9%, 17.2%, 25.9%, 33.8%, 36.1%, and 36.7%), were investigated as the nitrogen source for production of l-lactic acid by rhizopus oryzae nrrl-395 with respect to the influence of nitrogen source on l-lactic acid yield and the correlation with biomass yield and mycelia morphology. increases in crude protein from 0.21 to 1.68g/l led ... | 2010 | 20133131 |
| modulation of enzyme activities of a lead-adapted strain of rhizopus arrhizus during bioaccumulation of lead. | activity of some enzymes of a pb-adapted strain of rhizopus arrhizus augmented significantly during bioaccumulation of pb from a chemically defined medium. the mycelium of a pb-adapted strain exposed to 1 microg/ml pb in a defined medium for 10 d at 28 +/- 2 degrees c exhibited, relative to a wild-type strain, increased activities of antioxidant enzymes, i.e. sod, cat and gpx and of enzymes like ap and ppo involved in defense against pathogens. another response is a significant increase in the c ... | 2010 | 20140717 |
| correlation between in vitro activity of posaconazole and in vivo efficacy against rhizopus oryzae infection in mice. | we have evaluated the in vitro activity of posaconazole (psc) against 50 clinical strains of rhizopus oryzae using a broth microdilution method, the neo-sensitabs tablet diffusion method, and minimal fungicidal concentration (mfc) determination. in general, psc showed low mics against this fungus, and the mics correlated with the inhibition zone diameters. most of the mfcs, however, were from 1 to 4 dilutions higher than their corresponding mics. we then investigated the efficacies of several di ... | 2010 | 20145077 |
| structural homologies, importance for catalysis and lipid binding of the n-terminal peptide of a fungal and a pancreatic lipase. | lipases share an overall alpha/beta hydrolase fold structure characteristic of serine hydrolases. nevertheless, each lipases group possesses its characteristic 3-d structure and catalytic properties. the purified n-terminal truncated forms of a pancreatic (from ostrich) and a fungal (from rhizopus oryzae, rol32) (sayari et al., 2005) lipases displayed much lower activities as compared to the native proteins. the aim of this study is to explain this common functional feature on a structural basis ... | 2010 | 20214648 |
| a novel honeycomb matrix for cell immobilization to enhance lactic acid production by rhizopus oryzae. | a new support matrix inspired by honeycomb was developed for cell immobilization to control fungal morphology and enhance mass transfer in bioreactor for lactic acid production by rhizopus oryzae. the immobilization matrix composed of asterisk-shaped fibrous matrices in a honeycomb configuration provided high surface areas for cell attachment and biofilm growth. more than 90% of inoculated spores were adsorbed onto the matrices within 6-8h and after 10h there was no suspended cell in the ferment ... | 2010 | 20219359 |
| [effects of cultivation conditions on the optical purity of l(+)-lactic acid]. | the effect of cultivation conditions on the optical purity of l(+)-lactic acid produced by rhizopus oryzae hzs6 was investigated. the isomeric composition of lactic acid was influenced by the supplementation of l(+)-lactic acid to fermentation medium. l(+)-isomer increased with the dosage, no d(-)-lactic acid was observed when the concentration of supplemented l(+)-lactic acid in matrix was > or = 1.5 g/l. however, the l(+)-lactic acid yield, biomass and glucose conversion rate decreased with th ... | 2009 | 20222467 |
| strategies for utilisation of food-processing wastes to produce lipases in solid-state cultures of rhizopus oryzae. | the aim of the present study was to investigate the feasibility of several food-processing wastes as support substrate for lipolytic enzymes production by the fungus rhizopus oryzae under solid-state conditions. different experiments were conducted to select the variables that allow obtaining high levels of lipolytic enzyme activity. in particular, the use of inert and non-inert solid materials and lipidic and surfactant compounds was evaluated. it was observed that the addition of triton x-100 ... | 2010 | 20224920 |
| two-stage utilization of corn straw by rhizopus oryzae for fumaric acid production. | due to the abundant, low price characteristic, lots of efforts have been put into producing bulk chemical from lignocellulose biomass, but the low utility of xylose, which is the second main component in lignocellulose, becomes a bottleneck for efficient lignocellulose utilization. this study investigated a novel two-stage corn straw utilization strategy for fumaric acid production by rhizopus oryzae. fungal growth was approached in hydrolysates from acid hydrolysis of corn straw, contained 30 g ... | 2010 | 20236819 |
| heterologous expression and efficient ethanol production of a rhizopus glucoamylase gene in saccharomyces cerevisiae. | glucoamylases are inverting exo-acting starch hydrolases releasing β-glucose from the non-reducing ends of starch and related substrates. due to the absence of glucoamylase in saccharomyces cerevisiae, it is not capable of utilizing starch directly as energy sources without enzymatic or chemical hydrolysis for its ethanol production. in this study, we heterologously expressed a previously isolated rhizopus arrhizus glucoamylase gene in s. cerevisiae host. the expressed glucoamylase enzyme was se ... | 2011 | 20238168 |
| dependence on the properties of organic solvent: study on acyl migration kinetics of partial glycerides. | during rhizopus oryzae-mediated methanolysis of triglycerides for biodiesel production, the amount of 1,2-dg and 2-mg as well as the ratio of 1,2-dg/1,3-dg and 2-mg/1-mg differed significantly in different reaction medium, which indicated that solvent might be a crucial factor that would influence the acyl migration rate, leading to varied biodiesel yield. in this paper, the influence of solvent and their properties on acyl migration kinetics of both 1,2-diglyceride and 2-monoglyceride were inve ... | 2010 | 20307973 |
| surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media. | the cell surface engineering system, in which functional proteins are genetically displayed on microbial cell surfaces, has recently become a powerful tool for applied biotechnology. here, we report on the surfactant modification of surface-displayed lipase to improve its performance for enzymatic synthesis reactions. the lipase activities of the surfactant-modified yeast displaying rhizopus oryzae lipase (rol) were evaluated in both aqueous and nonaqueous systems. despite the similar lipase act ... | 2010 | 20336291 |
| effect of salinity on metal-complex dye biosorption by rhizopus arrhizus. | in this study the biosorption of yellow rl, a metal-complex anionic dye, by dried rhizopus arrhizus, a filamentous fungus, was investigated as a function of initial solution ph, initial dye concentration and initial salt (sodium chloride) concentration. the fungus exhibited the maximal dye uptake at ph 2 in the absence and in the presence of salt. dye uptake increased with the dye concentration up to 1000 mg l(-1) and diminished considerably in the presence of increasing concentrations of salt u ... | 2010 | 20347210 |
| molecular detection and identification of zygomycetes species from paraffin-embedded tissues in a murine model of disseminated zygomycosis: a collaborative european society of clinical microbiology and infectious diseases (escmid) fungal infection study group (efisg) evaluation. | the present study was performed to assess the interlaboratory reproducibility of the molecular detection and identification of species of zygomycetes from formalin-fixed paraffin-embedded kidney and brain tissues obtained from experimentally infected mice. animals were infected with one of five species (rhizopus oryzae, rhizopus microsporus, lichtheimia corymbifera, rhizomucor pusillus, and mucor circinelloides). samples with 1, 10, or 30 slide cuts of the tissues were prepared from each paraffi ... | 2010 | 20375233 |
| microbial synthesis of multishaped gold nanostructures. | the development of methodologies for the synthesis of nanoparticles of well-defined size and shape is a challenging one and constitutes an important area of research in nanotechnology. this full paper describes the controlled synthesis of multishaped gold nanoparticles at room temperature utilizing a simple, green chemical method by the interaction of chloroauric acid (haucl4 x 3h20) and cell-free extract of the fungal strain rhizopus oryzae. the cell-free extract functions as a reducing, shape- ... | 2010 | 20376859 |
| use of posaconazole in the treatment of infective rhinocerebral mucormycosis. | a patients with undiagnosed type ii diabetes mellitus presented with infective rhinocerebral mucormycosis. investigation results: initial magnetic resonance imaging scans demonstrated an aggressive disease process involving the left orbit and paranasal sinuses. a repeated scan following treatment excluded intracranial spread or recurrence. pus from the paranasal sinuses grew rhizopus arrhizus on microbiological culture. | 2010 | 20388246 |
| lipase immobilized on the hydrophobic polytetrafluoroethene membrane with nonwoven fabric and its application in intensifying synthesis of butyl oleate. | the synthesis of butyl oleate was studied in this paper with immobilized lipase. five types of membrane were used as support to immobilize rhizopus arrhizus lipase by following a procedure combining filtration and protein cross-linking. results showed that hydrophobic polytetrafluoroethene membrane with nonwoven fabric (ho-ptfe-nf) was the favorite choice in terms of higher protein loading, activity, and specific activity of immobilized lipase. the factors including solvent polarity, lipase dosa ... | 2010 | 20446058 |
| synthesis, characterization and antimicrobial studies of 2-(4-methoxy-phenyl)-5-methyl-4-(2-arylsulfanyl-ethyl)-2,4-dihydro-[1,2,4] triazolo-3-ones and their corresponding sulfones. | in the present investigation, a series of novel 2-(4-methoxy-phenyl)-5-methyl-4-(2-arylsulfanyl-ethyl)-2,4-dihydro-[1,2,4] triazolo-3-ones and their corresponding sulfones were prepared with the objective of developing better antimicrobial agents. the chemical structures of the newly synthesized compounds were characterized by spectral (ir, (1)h nmr, (13)c nmr and lcms) methods. the newly synthesized compounds (4a-i) and (5a-e, 5h) were screened for their antimicrobial activity against bacillus ... | 2010 | 20457477 |
| rearranged diterpenoids from the biotransformation of ent-trachyloban-18-oic acid by rhizopus arrhizus. | in our search for inhibitors of the antiapoptotic protein bcl-xl, investigation of xylopia caudata afforded a new diterpenoid, ent-trachyloban-4beta-ol (2), and five known ent-trachylobane or ent-atisane compounds. only ent-trachyloban-18-oic acid (1) exhibited weak binding activity to bcl-xl. these compounds exhibited cytotoxicity against kb and hct-116 cell lines with ic(50) values between 10 and 30 microm. bioconversion of compound 1 by rhizopus arrhizus afforded new hydroxylated metabolites ... | 2010 | 20481544 |
| the endothelial cell receptor grp78 is required for mucormycosis pathogenesis in diabetic mice. | mucormycosis is a fungal infection of the sinuses, brain, or lungs that causes a mortality rate of at least 50% despite first-line therapy. because angioinvasion is a hallmark of mucormycosis infections, we sought to define the endothelial cell receptor(s) for fungi of the order mucorales (the fungi that cause mucormycosis). furthermore, since patients with elevated available serum iron, including those with diabetic ketoacidosis (dka), are uniquely susceptible to mucormycosis, we sought to defi ... | 2010 | 20484814 |
| enhanced biocontrol activity of trichoderma virens transformants constitutively coexpressing beta-1,3- and beta-1,6-glucanase genes. | summary: evidence for the role of chitinases, proteases and beta-1,3- and beta-1,6-glucanases in mycoparasitism by trichoderma species has been well documented. moreover, constitutive over-expression of genes encoding individual cell-wall-degrading enzymes (cwdes) has been shown to improve the potential of biological agents. in this study, we generated transformants of t. virens in which beta-1,3- and beta-1,6-glucanase genes, tvbgn2 and tvbgn3, respectively, were constitutively coexpressed in t ... | 2007 | 20507514 |
| rhizopus oryzae skin infection treated with posaconazole in a boy with chronic granulomatous disease. | | 2010 | 20508483 |
| comparative study of the efficacy of liposomal amphotericin b and amphotericin b deoxycholate against six species of zygomycetes in a murine lethal infection model. | the objective of this study was to investigate the efficacy of liposomal amphotericin b (l-amb) at a clinical dose (3 mg/kg) against six species (5 genera) of zygomycetes in a murine lethal infection model, and to compare findings with those for deoxycholate amphotericin b (d-amb). the correlation between in-vitro activity and in-vivo efficacy of l-amb was also investigated. cyclophosphamide-treated mice were inoculated intravenously with conidial suspensions. four hours or 1 day after inoculati ... | 2010 | 20535522 |
| the high affinity iron permease is a key virulence factor required for rhizopus oryzae pathogenesis. | rhizopus oryzae is the most common cause of mucormycosis, an angioinvasive fungal infection that causes more then 50% mortality rate despite first-line therapy. clinical and animal model data clearly demonstrate that the presence of elevated available serum iron predisposes the host to mucormycosis. the high affinity iron permease gene (ftr1) is required for r. oryzae iron transport in iron-depleted environments. here we demonstrate that ftr1 is required for full virulence of r. oryzae in mice. ... | 2010 | 20545847 |
| catalytic properties of two rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in pichia pastoris. | catalytic properties of two glucoamylases, amyc and amyd, without starch binding domains from rhizopus oryzae strain 99-880 are determined using heterologously expressed enzyme purified to homogeneity. amyc and amyd demonstrate ph optima of 5.5 and 6.0, respectively, nearly one unit higher than the rhizopus amya glucoamylase enzyme. optimal initial activities are at 60 and 50 °c for amyc and amyd, respectively. inactivation of both enzymes occurs at 50 °c following 30 min pre-incubation. the two ... | 2010 | 20549574 |
| efficient production of lactic acid from sucrose and corncob hydrolysate by a newly isolated rhizopus oryzae gy18. | the aim of this study is to investigate production of l-lactic acid from sucrose and corncob hydrolysate by the newly isolated r. oryzae gy18. r. oryzae gy18 was capable of utilizing sucrose as a sole source, producing 97.5 g l(-1) l-lactic acid from 120 g l(-1) sucrose. in addition, the strain was also efficiently able to utilize glucose and/or xylose to produce high yields of l-lactic acid. it was capable of producing up to 115 and 54.2 g l(-1) lactic acid with yields of up to 0.81 g g(-1) glu ... | 2010 | 20556475 |
| using of rhizopus arrhizus as a sensor modifying component for determination of pb(ii) in aqueous media by voltammetry. | for the sensitive determination of pb(ii) from aqueous solutions, a new voltammetric biosensor based on carbon paste electrode modified with rhizopus arrhizus was developed. the preconcentrated ions at open circuit were reduced by using differential pulse stripping voltammetry technique. the obtained current values were related to the concentration of pb(ii) in the solutions. the best results were achieved at ph 7 with 0.01 m tris-hcl buffer solution applying a preconcentration time of 12 min. t ... | 2010 | 20576569 |
| effect of different stresses on trehalose levels in rhizopus oryzae. | rhizopus oryzae accumulates and degrades trehalose in response to environmental stress conditions such as heat, osmotic stress, nitrogen starvation and ph. when heat stress was applied to r. oryzae, the trehalose content of the cells was increased from 0.9 to 4.8 mg/g dw and when the stress was relieved it decreased back to 1.1 mg/g dw. under osmotic stress and nitrogen starvation, trehalose content was increased by 3.5 and 3 fold, respectively. the decrease in external ph increased trehalose le ... | 2010 | 20586064 |
| chitosan production from hemicellulose hydrolysate of corn straw: impact of degradation products on rhizopus oryzae growth and chitosan fermentation. | to examine the potential use of hemicellulose hydrolysate (hh) for the production of chitosan by rhizopus oryzae and investigate the influence of contents in hh on mycelia growth and chitosan synthesis. | 2010 | 20636326 |
| in vitro and in vivo activities of posaconazole against zygomycetes with various degrees of susceptibility. | we analysed the in vitro and in vivo effects of posaconazole and amphotericin b against three clinical isolates of zygomycetes: lichtheimia corymbifera, f1; and rhizopus oryzae, f5 and f6. | 2010 | 20667887 |
| enzyme mediated silicon-oxygen bond formation; the use of rhizopus oryzae lipase, lysozyme and phytase under mild conditions. | the potential for expanding the variety of enzymic methods for siloxane bond formation is explored. three enzymes, rhizopus oryzae lipase (rol), lysozyme and phytase are reported to catalyse the condensation of the model compound, trimethylsilanol, formed in situ from trimethylethoxysilane, to produce hexamethyldisiloxane in aqueous media at 25 °c and ph 7. thermal denaturation and reactant inhibition experiments were conducted to better understand the catalytic role of these enzyme candidates. ... | 2010 | 20683529 |
| evaluation of tertiary treatment by fungi, enzymatic and photo-fenton oxidation on the removal of phenols from a kraft pulp mill effluent: a comparative study. | pulp and paper mills generate pollutants associated to their effluents depending upon the type of process, type of the wood materials, process technology applied, management practices, internal recirculation of the effluent for recovery, the amount of water used in the industrial process and type of secondary treatment. this study is the first that reports a simultaneous evaluation of the effects of tertiary treatments by fungi (rhizopus oryzae and pleurotus sajor caju), by enzyme (laccase) and ... | 2011 | 20683764 |
| lessons from a case of oromandibular mucormycosis treated with surgery and a combination of amphotericin b lipid formulation plus caspofungin. | a rare case of oromandibular rhizopus oryzae infection is described in a 55-year-old woman with acute myeloid leukaemia and decompensated diabetes mellitus. the infection developed during induction chemotherapy when the patient was neutropenic. she was treated with a combination of amphotericin b lipid formulation and caspofungin plus surgery. debridement surgery included excision of the lower lip, chin, floor of the mouth, a portion of the tongue, as well as mandibular resection at the level of ... | 2010 | 20689269 |
| improved oxygen transfer and increased l-lactic acid production by morphology control of rhizopus oryzae in a static bed bioreactor. | rhizopus oryzae was immobilized on a cotton matrix in a static bed bioreactor. compared with free cells in a stirred tank bioreactor, immobilized r. oryzae in this bioreactor gave higher lactic acid production but lower ethanol production. the highest lactic acid production rate (2.09 g/l h) with the final concentration of 37.83 g/l from 70 g/l glucose was achieved when operating the bioreactor at 700 rpm and 0.5 vvm air. to better understand the relationship between shear effects (agitation and ... | 2011 | 20703501 |
| multilocus microsatellite typing for rhizopus oryzae. | rhizopus oryzae is the most frequent causative agent of zygomycosis. although zygomycosis causes considerable morbidity and mortality in immunocompromised patients, the epidemiology of the disease is not well studied and no standard molecular typing method has been described for any of the causative agents. here we describe a multilocus microsatellite typing (mlmt) method for r. oryzae. r. oryzae genome sequences were downloaded from the fungal genome initiative database (broad institute). the i ... | 2010 | 20724504 |
| [a case of resected pulmonary mucormycosis that was suspected of mixed infection with aspergillus fumigatus, diagnosed by transbronchial lung biopsy]. | a 73-year-old man was admitted with high fever and right chest pain. chest x-ray showed a rapidly growing mass shadow in the right lower lung field. the patient had been in remission for malignant lymphoma and had developed interstitial pneumonia and diabetes mellitus following 1 year of corticosteroid therapy. his illness was diagnosed as invasive aspergillosis because of a high level of beta-d-glucan and cultured aspergillus fumigatus in the sputum. he was treated with a combination of micafun ... | 2010 | 20803983 |
| biodiesel production by a mixture of candida rugosa and rhizopus oryzae lipases using a supercritical carbon dioxide process. | in this study, various factors, such as temperature, pressure, agitation speed, water content, and the concentration and ratio of immobilized rol and crl were investigated for the efficient enzymatic production of biodiesel using a supercritical carbon dioxide process. furthermore, a stepwise reaction method for the maintenance of immobilized lipase activity was optimized. optimal conditions for biodiesel production were determined to be as follows: 130 bar pressure, 45 °c temperature, 250 rpm a ... | 2011 | 20813518 |
| production of l-lactic acid by rhizopus oryzae using semicontinuous fermentation in bioreactor. | semicontinuous fermentation using pellets of rhizopus oryzae has been recognized as a promising technology for l-lactic acid production. in this work, semicontinuous fermentation of r. oryzae as 3.819 for l-lactic acid production has been developed with high l-lactic acid yield and volumetric productivity. the effects of factors such as inoculations, caco₃ addition time, and temperature on l-lactic acid yield and r. oryzae morphology were researched in detail. the results showed that optimal fer ... | 2011 | 20824489 |
| [case of a diabetic man cured of rhinocerebral zygomycosis]. | zygomycosis (mucormycosis) is a rare, highly aggressive opportunistic fungal disease caused by saprophytic fungi, belonging to the division zygomycota, class zygomycetes. patients with immunodeficiency, neutropenia, iron overload, hematological malignancies, as well as diabetics with ketoacidosis are typically affected. case presentation: authors describe the case of an eighteen-year old man with poor compliance suffering from diabetes since the age of nine. he was admitted with ketoacidotic som ... | 2010 | 20840916 |
| acid phosphatase production by rhizopus delemar: a role played in the ni(ii) bioaccumulation process. | the microbial growth and activity of acid phosphatase enzyme during the growth of rhizopus delemar in the presence or absence of ni(ii) ions were investigated. an increase in initial ni(ii) ion concentration inhibited both growth rate of r. delemar and acid phosphatase activity. the maximum-intrinsic specific growth rate (μ(m)) and monod constant (k(s)) of microorganism in ni(ii)-free medium were found as 0.0649 h(-1) and 1.8928 g/l, respectively. the inhibition of ni(ii) ions on growth rate of ... | 2010 | 20846788 |
| discovery of a steroid 11α-hydroxylase from rhizopus oryzae and its biotechnological application. | to overcome the chemically laborious stereo- and regioselective hydroxylation steps in the pharmaceutical production of corticosteroids and progestogens, certain fungal species, e.g. rhizopus spp. and aspergillus spp., are employed to perform the 11α-hydroxylation of the steroid skeleton, thereby significantly simplifying steroid drug production. here we report for the first time the identification and expression of a fungal 11α-steroid hydroxylase, cyp509c12. the newly identified cytochrome p45 ... | 2010 | 20850485 |
| enantioselective production of benzoin from benzoin acetate via kinetic resolution and deracemization using rhizopus oryzae. | abstract: in this study, the production of enantiopure benzoin from rac-benzoin acetate was achieved by lipase catalyzed kinetic resolution combined with deracemization using rhizopus oryzae (cbs111718). the growth cells were pretreated with 20 khz and 30 khz ultrasound irradiation and mechanical homogenization. approximately 100% conversion and 96% enantiomeric excess of the product (s-benzoin) were obtained by applying 20 khz ultrasound irradiation at ph 6. the deracemization process involves ... | 2011 | 20858042 |
| basilar artery occlusion after multifactor coagulopathy including rhizopus oryzae infection in burns. | the authors report a case of a 29-year-old male patient with severe burn injuries (54% tbsa) and inhalation injury. he developed a candidemia and a cutaneous zygomycotic superinfection with rhizopus oryzae while he received burn intensive care. despite aggressive surgery, sepsis persisted, and therapy was limited by uncontrollable coagulopathy and catecholamine refractory shock after 15 days. autopsy revealed a thromboembolic occlusion of the basilar artery that resulted in liquefactive necrosis ... | 2010 | 20859211 |
| the mycobiota from seeds of shrunken-2 (sh2) sweet corn. | sixteen species of fungi were isolated from the pericarp, endosperm, scutellum, and embryo of low (<75% germination), moderate (75-89%), and high vigor (>89%) seedlots of supersweet sweet corn (sh2) hybrids over two years. most frequently isolated species werepenicillium oxalicum, rhizopus arrhizus (14.5%), andrhizopus spp (17.4%).fusarium moniliforme, a common inhabitant in field or dent corn, was isolated from only 2.4% of the samples. the low mean isolation values may be the result of poor co ... | 1995 | 20882448 |
| structure, function, and phylogeny of the mating locus in the rhizopus oryzae complex. | the rhizopus oryzae species complex is a group of zygomycete fungi that are common, cosmopolitan saprotrophs. some strains are used beneficially for production of asian fermented foods but they can also act as opportunistic human pathogens. although r. oryzae reportedly has a heterothallic (+/-) mating system, most strains have not been observed to undergo sexual reproduction and the genetic structure of its mating locus has not been characterized. here we report on the mating behavior and genet ... | 2010 | 21151560 |
| expression and characterization of fifteen rhizopus oryzae 99-880 polygalacturonase enzymes in pichia pastoris. | polygalacturonase (pg) enzymes hydrolyze the long polygalacturonic acid chains found in the smooth regions of pectin. interest in this enzyme class continues due to their ability to macerate tissues of economically important crops and their use in a number of industrial processes. rhizopus oryzae has a large pg gene family with 15 of 18 genes encoding unique active enzymes. the pg enzymes, 12 endo-pg and 3 exo-galacturonases, were expressed in pichia pastoris and purified enabling biochemical ch ... | 2010 | 21161225 |
| pyogenic liver abscess and peritonitis due to rhizopus oryzae in a child with papillon-lefevre syndrome. | papillon-lefevre syndrome (pls) is an autosomal recessive disease that is characterized by symmetric palmoplantar keratodermatitis and severe periodontal destruction. mutations in the cathepsin c gene (ctsc) have recently been detected in pls. immune dysregulation, due to a mutation in ctsc, increases the risk of pyogenic infections in pls patients. a child with pls is presented here with liver abscesses and peritonitis caused by rhizopus oryzae. his liver abscess and peritonitis were cured with ... | 2010 | 21165748 |
| a large scale enzyme screen in the search for new methods of silicon-oxygen bond formation. | biotransformations make use of biological systems to catalyze or promote specific chemical reactions. transformations that utilize enzymes as "greener" and milder catalysts compared to traditional reaction conditions are of particular interest. recently, organosilicon compounds have begun to be explored as non-natural enzymatic substrates for biotransformations. the aims of this study were to screen readily available (approximately eighty) enzymes for their ability to catalyze in vitro siloxane ... | 2010 | 21194627 |
| identification and partial characterization of rhizopus nigricans gβ proteins and their expression in the presence of progesterone. | the mammalian steroid hormone progesterone actuates a signalling pathway in the zygomycete rhizopus nigricans which includes heterotrimeric g proteins. to investigate the possibility that the gβ subunit of these proteins is involved in the signalling, a cdna library from r. nigricans exposed to progesterone was prepared and a sequence coding for a gβ subunit was searched for. using degenerate primers, two sequences, rngpb1 and rngpb2, were identified that exhibited a high degree of identity with ... | 2010 | 21195176 |
| expression and characterization of fumarase (fumr) from rhizopus oryzae. | fumarase catalyzes the reversible hydration of fumarate to l-malate in rhizopus oryzae. a recombinant pet22b-fumr harboring a fumarase gene (fumr) from r. oryzae was constructed for high level expression in e. coli bl21 (de3). the fumr activity was optimal at 30°c and ph 7.2. the enzyme was stable below 45°c and at ph 3.0-9.0. no effects of zn(2+), fe(2+), or edta were observed on enzyme activity. a slight inhibition of fumr activity was seen with mg(2+), while ca(2+) had a small stimulatory eff ... | 2010 | 21215954 |
| carbohydrate-active enzymes from the zygomycete fungus rhizopus oryzae: a highly specialized approach to carbohydrate degradation depicted at genome level. | rhizopus oryzae is a zygomycete filamentous fungus, well-known as a saprobe ubiquitous in soil and as a pathogenic/spoilage fungus, causing rhizopus rot and mucomycoses. | 2011 | 21241472 |
| gene cloning, heterologous expression, and characterization of a high maltose-producing α-amylase of rhizopus oryzae. | a putative α-amylase gene, designated as roamy, was cloned from rhizopus oryzae. the deduced amino acid sequence showed the highest (42.8%) similarity to the α-amylase from trichoderma viride. the roamy gene was successfully expressed in pichia pastoris gs115 under the induction of methanol. the molecular weight of the purified roamy determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis was approximately 48 kda. the optimal ph and temperature were 4-6 and 60 °c, respectively. t ... | 2011 | 21243443 |
| isolation, identification and optimization of a new extracellular lipase producing strain of rhizopus sp. | a lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. based on its1-5.8s-its4 region sequences of ribosomal rna, it was concluded that the isolate jk-1 belongs to genus rhizopus and clades with rhizopus oryzae. the present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (ec 3.1.1.3). culture conditions were optimized, and the highest lipase production was observed in basal mediu ... | 2011 | 21302143 |
| simultaneous detection and identification of aspergillus and mucorales species in tissues collected from patients with fungal rhinosinusitis. | rapid detection and differentiation of aspergillus and mucorales species in fungal rhinosinusitis diagnosis are desirable, since the clinical management and prognosis associated with the two taxa are fundamentally different. we describe an assay based on a combination of broad-range pcr amplification and reverse line blot hybridization (pcr/rlb) to detect and differentiate the pathogens causing fungal rhinosinusitis, which include five aspergillus species (a. fumigatus, a. flavus, a. niger, a. t ... | 2011 | 21325541 |
| mechanism of acetaldehyde-induced deactivation of microbial lipases. | microbial lipases represent the most important class of biocatalysts used for a wealth of applications in organic synthesis. an often applied reaction is the lipase-catalyzed transesterification of vinyl esters and alcohols resulting in the formation of acetaldehyde which is known to deactivate microbial lipases, presumably by structural changes caused by initial schiff-base formation at solvent accessible lysine residues. previous studies showed that several lipases were sensitive toward acetal ... | 2011 | 21342514 |
| production of fumaric acid by rhizopus oryzae: role of carbon-nitrogen ratio. | cytosolic fumarase, a key enzyme for the accumulation of fumaric acid in rhizopus oryzae, catalyzes the dehydration of l-malic acid to fumaric acid. the effects of carbon-nitrogen ratio on the acid production and activity of cytosolic fumarase were investigated. under nitrogen limitation stress, the cytosolic fumarase could keep high activity. with the urea concentration decreased from 2.0 to 0.1 g l?¹, the cytosolic fumarase activity increased by 300% and the production of fumaric acid increase ... | 2011 | 21416336 |
| in vivo regulation of alcohol dehydrogenase and lactate dehydrogenase in rhizopus oryzae to improve l-lactic acid fermentation. | rhizopus oryzae is becoming more important due to its ability to produce an optically pure l: -lactic acid. however, fermentation by rhizopus usually suffers from low yield because of production of ethanol as a byproduct. limiting ethanol production in living immobilized r. oryzae by inhibition of alcohol dehydrogenase (adh) was observed in shake flask fermentation. the effects of adh inhibitors added into the medium on the regulation of adh and lactate dehydrogenase (ldh) as well as the product ... | 2011 | 21416338 |
| transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing rhizopus oryzae cells as whole-cell biocatalyst. | the enzymatic process presents an advantage of producing specified phospholipids that rarely exist in nature. in this study, we investigated the regiospecific modification of phosphatidylcholine (pc) in the sn-1 position using immobilized rhizopus oryzae. in a reaction mixture containing egg yolk pc and exogenous lauric acid (la) in n-hexane, lipase-producing r. oryzae cells immobilized within biomass support particles (bsps) showed a much higher transesterification activity than lipase powders. ... | 2011 | 21468705 |
| pentachlorophenol sorption in nylon fiber and removal by immobilized rhizopus oryzae enhe. | this study describes pentachlophenol (pcp) sorption in nylon fiber in which rhizopus oryzae enhe was immobilized to remove the chemical compound. the experimental sorption data were analyzed using the langmuir, freundlich, and redlich-peterson isotherm models using non-linear error functions to fit the experimental data to the three models. results showed that the isotherm obtained from the data fitted the three models used. however, the g parameter from redlich-peterson model showed that the is ... | 2011 | 21514996 |
| efficacy of caspofungin in neutropenic and corticosteroid-immunosuppressed murine models of invasive pulmonary mucormycosis. | caspofungin (cfg) was tested in neutropenic and corticosteroid-immunosuppressed mice challenged with a lethal sinopulmonary inoculum of rhizopus oryzae. compared to untreated controls, cfg 1 mg/kg/day significantly improved survival (54% vs.19%, p=0.003) and reduced median r. oryzae fungal burden by 1.5 log(10) condial equivalent dna in neutropenic, but not corticosteroid-immunosuppressed animals. cfg 16 mg/kg/day was not significantly better than saline control for treatment of ipm in either ne ... | 2011 | 21537029 |
| lipase activity in insect oral secretions mediates defense responses in arabidopsis thaliana. | how plants perceive herbivory is not yet well understood. we investigated early responses of the model plant arabidopsis thaliana to attack from the generalist grasshopper herbivore, schistocerca gregaria (caelifera). when compared to wounding alone, schistocerca gregaria attack and the application of grasshopper oral secretions (gs) to puncture wounds elicited a rapid accumulation of various oxylipins, including 13-hydroperoxy octadecatrienoic acid, 12-oxo-phytodienoic acid (opda), jasmonic aci ... | 2011 | 21546453 |
| rhinocerebral zygomycosis in a diabetic patient. | rhinocerebral zygomycosis is the most frequent form of fungal infection caused by members of the zygomycetes class. a fatal case of rhinocerebral zygomycosis caused by rhizopus (oryzae) arrhizus with histopathological and mycological diagnosis is reported in a diabetic patient. | 2011 | 21552748 |
| successful treatment of rhino-orbital mucormycosis by a new combination therapy with liposomal amphotericin b and micafungin. | mucormycosis is a rapidly progressive fungal infection that usually occurs in patients with diabetes mellitus or in immunocompromised patients. sinus involvement is the most common clinical presentation and the rates of mortality increase with the orbital extension. the treatment of mucormycosis includes aggressive surgical debridement and systemic antifungal therapy. early diagnosis and prompt initiation of effective antifungal drugs are essential for successful outcome. however, the role of or ... | 2011 | 21592699 |
| improved expression of rhizopus oryzae α-amylase in the methylotrophic yeast pichia pastoris. | in our previous study, the α-amylase from rhizopus oryzae (roamy) was expressed in escherichia coli and saccharomyces cerevisiae but the obtained recombinant roamy (rroamy) yields were too low. the aim of the present research was to obtain high-level expressions of roamy in the methylotrophic yeast pichia pastoris. to this end, we constructed p. pastoris strains with the capability to express recombinant α-amylase under the control of constitutive glyceraldehyde-3-phosphate dehydrogenase (gap) a ... | 2011 | 21616151 |
| biofilm formation by zygomycetes: quantification, structure and matrix composition. | most of the studies on fungal biofilms have focused on candida in yeasts and aspergillus in mycelial fungi. biofilm formation by zygomycetes has not been reported yet. in this study, we evaluated the biofilm-forming capacity of rhizopus oryzae, lichtheimia corymbifera, rhizomucor pusillus and apophysomyces elegans. at appropriate seeding spore densities, rh. oryzae (105 cfu ml-1), l. corymbifera (104 cfu ml-1) and r. pusillus (104 cfu ml-1) produced highly intertwined, adherent structures on fla ... | 2011 | 21636650 |
| pulmonary zygomycosis: a clinicopathological study. | zygomycosis is an emerging infection worldwide. pulmonary zygomycosis (pz) is uncommon with only few reported series from india. | 2011 | 21654982 |
| [metabolism of rhizopus oryzae with xylose or glucose as carbon resource]. | as one of the most abandon and cheapest recyclable bio-resources, cellulosic biomass appear to be the promising new energy if the two major components, glucose and xylose, are efficiently used. | 2011 | 21796980 |
| in vitro interaction of caspofungin and immunosuppressives against agents of mucormycosis. | objectives mucormycosis is a rare but refractory mycosis with high mortality. few therapeutic options are available and novel strategies are needed. calcineurin inhibitors are known to have antifungal activity, including synergy with various antifungals. we examined the interaction of caspofungin with calcineurin inhibitors and sirolimus against glomeromycetes. methods twenty-six strains of glomeromycetes representing seven species (rhizopus arrhizus, rhizopus microsporus, mucor sp., rhizomucor ... | 2011 | 21795260 |
| voriconazole pre-exposure selects for breakthrough mucormycosis in a mixed model of aspergillus fumigatus-rhizopus oryzae pulmonary infection. | mucormycosis is an uncommon fungal infection that has been increasingly reported in severely immunocompromised patients receiving aspergillus-active antifungals. although clinical studies and pre-clinical animal models have suggested a unique predisposition for breakthrough mucormycoses in patients receiving voriconazole, no study has specifically evaluated the selection dynamics of various aspergillus -active antifungal classes in vivo. we utilized an aspergillus fumigatus:rhizopus oryzae (10:1 ... | 2011 | 21788730 |
| rna silencing of lactate dehydrogenase gene in rhizopus oryzae. | rhizopus oryzae is a filamentous fungus, belonging to the order mucorales. it can ferment a wide range of carbohydrates hydrolyzed from lignocellulosic materials and even cellobiose to produce ethanol. however, r. oryzae also produces lactic acid as a major metabolite, which reduces the yield of ethanol. in this study, we show that significant reduction of lactic acid production could be achieved by short (25nt) synthetic sirnas targeting the ldha gene. the average yield of lactic acid productio ... | 2011 | 21769297 |
| a case of rhinoorbital mucormycosis in a leukemic patient with a literature review from turkey. | mucormycosis (zygomycosis) is a rare, invasive, opportunistic fungal infection of the paranasal sinuses, caused by a fungus of the order mucorales. we report a case of rhinoorbital mucormycosis caused by rhizopus oryzae in an acute lymphoblastic leukemia patient and review the 79 mucormycosis cases reported in the last decade from turkey. in our case, the diagnosis was made with endoscopic appearance, computerized tomography of the paranasal sinuses, and culture of the surgical materials. follow ... | 2011 | 21761152 |
| immobilized rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: optimization by response surface methodology. | waxes are esters of long-chain fatty acids and long-chain alcohols. their principal natural sources are animals (sperm whale oil) and vegetables (jojoba) which are expensive and not easily available. wax esters synthesized by enzymatic transesterification, using palm stearin as raw material, can be considered as an alternative to natural ones. | 2011 | 21682865 |
| a statistical approach for optimization of simultaneous production of +¦-glucosidase and endoglucanase by rhizopus oryzae from solid-state fermentation of water hyacinth using central composite design. | the production cost of +¦-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (ssf). in the present study, the effect of different production conditions on the yield of +¦-glucosidase and endoglucanase by rhizopus oryzae mtcc 9642 from water hyacinth was investigated systematically using response surface methodology. a central composite experimental design ... | 2011 | 21687577 |
| changes in lipid, fatty acids and phospholipids composition of whole rice bran after solid-state fungal fermentation. | the aim of this study was to evaluate fermented rice bran phospholipids, lipids and fatty acid content in a fermentation solid system with rhizopus oryzae fungus. for this, aliquots were withdrawn every 24h over 120h. the content of phospholipids was determined by colorimetric method. esterified fatty acids were separated by gas chromatography, then identified and quantified. the total lipids from fermented rice bran (fb) decreased from 20.4% to 11.2% in the range between 0h and 120h of fermenta ... | 2011 | 21715163 |
| construction of the yeast whole-cell rhizopus oryzae lipase biocatalyst with high activity. | surface display is effectively utilized to construct a whole-cell biocatalyst. codon optimization has been proven to be effective in maximizing production of heterologous proteins in yeast. here, the cdna sequence of rhizopus oryzae lipase (rol) was optimized and synthesized according to the codon bias of saccharomyces cerevisiae, and based on the saccharomyces cerevisiae cell surface display system with +¦-agglutinin as an anchor, recombinant yeast displaying fully codon-optimized rol with high ... | 2011 | 21726061 |
| fungi isolated from olive ecosystems and screening of their potential biotechnological use. | this study investigated the fungi diversity of fresh olive (olea europaea l.) fruits, olive paste (crushed olives) and olive pomace (solid waste) and screened and quantified enzymatic activities with biotechnological applications. fungi were randomly isolated from olive cultivars from castilla la mancha region (spain). identification included comparison of their polymerase chain reaction (pcr) amplicons of the its1-5.8s-its2 ribosomal dna region, followed by nucleotide sequence analysis. fourtee ... | 2011 | 21689797 |
| immobilization and stability of a rhizopus oryzae lipase expressed in pichia pastoris: comparison between native and recombinant variants. | the stability of a soluble extract containing a recombinant lipase from rhizopus oryzae (cursive) lipase (rrol) produced by pichia pastoris (cursive), as well as that for the commercial extract containing the lipase produced by the native organism (nrol), was investigated. the results showed higher residual activity values of the commercial protein compared with the recombinant one. moreover, two different kinds of support, the polypropylene powder ep100 and eupergit®c, were tested to immobilize ... | 2011 | 21681981 |
| optimization of fumaric acid production by rhizopus delemar based on the morphology formation. | the effects of temperature, agitation rate and medium composition, including concentrations of glucose, soybean peptone, and inorganic ions, on pellet formation and pellet diameter of rhizopus delemar (rhizopus oryzae) nrrl1526 during pre-culture were studied. inorganic ions and soybean peptone had negative and positive effects on pellet formation, respectively. the initial glucose and soybean peptone concentrations directly affected pellet diameter. within a certain range, pellet diameter decre ... | 2011 | 21880482 |
| production of cyanophycin in rhizopus oryzae through the expression of a cyanophycin synthetase encoding gene. | cyanophycin or cyanophycin granule peptide is a protein that results from non-ribosomal protein synthesis in microorganisms such as cyanobacteria. the amino acids in cyanophycin can be used as a feedstock in the production of a wide range of chemicals such as acrylonitrile, polyacrylic acid, 1,4-butanediamine, and urea. in this study, an auxotrophic mutant (rhizopus oryzae m16) of the filamentous fungus r. oryzae 99-880 was selected to express cyanophycin synthetase encoding genes. these genes o ... | 2011 | 21972133 |
| Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide. | In present work, Rhizopus oryzae lipase immobilized on a film prepared using blend of hydroxylpropyl methyl cellulose (HPMC) and polyvinyl alcohol (PVA) was investigated for synthesis of citronellol esters with supercritical carbon dioxide (Sc-CO(2)) as a reaction medium. The transesterification reaction was optimized for various reaction parameters like effect of molar ratio, acyl donor, time, temperature, enzyme concentration, effect of pressure and co-solvent to achieve the maximum yield of d ... | 2011 | 21884733 |
| Expression and characterization of recombinant Rhizopus oryzae lipase for enzymatic biodiesel production. | The Rhizopus oryzae lipase containing prosequence was expressed in Pichia pastoris. Recombinant lipase subunit showed a molecular mass of 32 kDa. The maximum activity of recombinant lipase obtained from Mut(s) recombinant was 90 IU/ml. The enzyme was stable in broad ranges of temperatures and pH, with the optimal temperature at 35 °C and pH 7.0. The crude recombinant R. oryzae lipase can be directly used for the transesterification of plant oils at high-water content of 60-100% (w/w) based on oi ... | 2011 | 21852124 |
| a case of intestinal mucormycosis in a common marmoset (callithrix jacchus). | a 3-year-old female common marmoset was euthanatized because of wasting. grossly, a perforating lesion was present in the jejunum and hemorrhagic lesions in the cecum and colon. histopathologically, these gross lesions were a perforated jejunal ulcer and necrotizing colitis, respectively. necrotizing colitis was characterized by extensive mucosal necrosis along with numerous ribbon-shaped aseptate hyphae. these aseptate hyaline hyphae were positively stained with pas and gms, and reacted immunoh ... | 2011 | 22020147 |
| Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts and revolutionized the daily clinical laboratory routine. But it has not been established for routine mould identification. This study aimed to validate a standardized procedure for MALDI-TOF MS-based mould identification in clinical laboratory. | 2011 | 22194834 |
| stability of lipase immobilized on o-pentynyl dextran. | o-pentynyl dextran (pyd), an amphiphilic polysaccharide derivative with a degree of substitution (ds) of 0.43 was compared with ion exchange resins lewatit vp oc 1600, amberlite xad 761 and duolite a568 for immobilization of lipase from rhizopus arrhizus by adsorption method. the immobilized enzymes were employed for esterification of octanoic acid with geraniol in n-hexane as model reaction. pyd showed higher lipase adsorption and with 249 μmol min(-1 )g(-1) significant higher esterification ac ... | 2011 | 21932061 |
| entrapment of mycelial fragments in calcium alginate: a general technique for the use of immobilized filamentous fungi in biocatalysis. | transformation reactions on 3β,17β-dihydroxyandrost-5-ene using free fungal cells were compared with those carried out by macerated mycelia, immobilized in calcium alginate beads. six fungi were utilized in this study, namely rhizopus oryzae atcc 11145, mucor plumbeus atcc 4740, cunninghamella echinulata var. elegans atcc 8688a, aspergillus niger atcc 9142, phanerochaete chrysosporium atcc 24725 and whetzelinia sclerotiorum atcc 18687. the results show, for the first time, that encapsulated myce ... | 2012 | 22064215 |
| Alternative routes of acetyl-CoA synthesis identified by comparative genomic analysis: involvement in the lipid production of oleaginous yeast and fungi. | For a bio-based economy, microbial lipids offer a potential solution as alternative feedstocks in the oleochemical industry. The existing genome data for the promising strains, oleaginous yeasts and fungi, allowed us to investigate candidate orthologous sequences that participate in their oleaginicity. Comparative genome analysis of the non-oleaginous (Saccharomyces cerevisiae, Candida albicans and Ashbya gossypii) and oleaginous strains (Yarrowia lipolytica, Rhizopus oryzae, Aspergillus oryzae ... | 2012 | 22016567 |
| Production of phytase under solid-state fermentation using Rhizopus oryzae: novel strain improvement approach and studies on purification and characterization. | Present study introduces linseed oil cake as a novel substrate for phytase production by Rhizopus oryzae. Statistical approach was employed to optimize various medium components under solid state fermentation (SSF). An overall 8.41-fold increase in phytase production was achieved at the optimum concentrations (w/w, mannitol, 2.05%; ammonium sulfate, 2.84% and phosphate, 0.38%). Further enhancement by 59% was observed due to a novel strain improvement approach. Purified phytase (~34 kDa) showed o ... | 2011 | 21945206 |
| production of recombinant rhizopus oryzae lipase by the yeast yarrowia lipolytica results in increased enzymatic thermostability. | the gene encoding rhizopus oryzae lipase (rol) was expressed in the non-conventional yeast yarrowia lipolytica under the control of the strong inducible xpr2 gene promoter. the effects of three different preprosequence variants were examined: a preprosequence of the y. lipolytica alkaline extracellular protease (aep) encoded by xpr2, the native preprosequence of rol, and a hybrid variant of the presequence of aep and the prosequence of rol. lipase production was highest (7.6u/ml) with the hybrid ... | 2011 | 22155648 |
| Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media. | Water activity (a(w)) is a crucial parameter affecting enzymatic synthetic reactions in organic media. In this paper, we report on the a(w) dependence of surface-displayed lipases, genetically immobilized on yeast cells via fusion with cell wall proteins. When Saccharomyces cerevisiae displaying Rhizopus oryzae lipase was used for esterification in n-hexane, equilibrating the dried cells with water prior to the reaction markedly increased the reaction rate. An equilibration of the cells with var ... | 2011 | 22112946 |
| molecular and morphological identification of fungal species isolated from bealmijang meju. | bealmijang is a short-term aged paste made from meju, which is a brick of fermented soybeans and other ingredients. different types of bealmijang are available depending on the geographic region or ingredients used. however, no study has clarified the microbial diversity of these types. we identified 17 and 14 fungal species from black soybean meju (bsm) and buckwheat meju (bwm), respectively, on the basis of morphology, culture characteristics, and internal transcribed spacer and beta-tubulin g ... | 2011 | 22210613 |
| data partitions, bayesian analysis and phylogeny of the zygomycetous fungal family mortierellaceae, inferred from nuclear ribosomal dna sequences. | although the fungal order mortierellales constitutes one of the largest classical groups of zygomycota, its phylogeny is poorly understood and no modern taxonomic revision is currently available. in the present study, 90 type and reference strains were used to infer a comprehensive phylogeny of mortierellales from the sequence data of the complete its region and the lsu and ssu genes with a special attention to the monophyly of the genus mortierella. out of 15 alternative partitioning strategies ... | 2011 | 22102902 |