| a maldi-tof ms procedure for clinical dermatophyte species identification in the routine laboratory. | the conventional identification of dermatophytes requires a long turnaround time and highly skilled mycologists. we have recently developed a tandardized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) assay to routinely identify molds of potential clinical significance. this study objective was to determine if this same assay could also be employed to identify clinical dermatophytes in the routine laboratory setting. the effects of the inclusion of cy ... | 2013 | 23611419 |
| genetic variability in microsporum canis isolated from cats, dogs and humans in brazil. | dermatophytosis caused by microsporum canis is a heterogeneous disease with variable clinical manifestations. m. canis is a zoophilic dermatophyte and the most frequent fungi isolated from dogs, cats and children in brazil. the aim of this study was to investigate the genetic variability of m. canis isolates from different animal species using two microsatellite markers, namely, mcgt(13) and mcgt(17), and to correlate the results with the clinical and epidemiological patient data in brazil. the ... | 2013 | 23551796 |
| development and validation of a microsatellite marker-based method for tracing infections by microsporum canis. | microsporum canis is a dermatophyte fungus harbored by cats and dogs and is frequently transmitted to humans. molecular tools able to discriminate fungal isolates at the strain level would prove extremely useful for confirming the route of infection, thus contributing to optimization of prophylaxis and hygienic regimens. | 2013 | 23415957 |
| microsatellite-primed pcr and random primer amplification polymorphic dna for the identification and epidemiology of dermatophytes. | this study demonstrates the capacity of the one-step polymerase chain reaction (pcr) fingerprinting method using the microsatellite primers (gaca)4 or (gtg)5 (msp-pcr) to identify six of the most frequent dermatophyte species causing cutaneous mycosis. pcr with (gaca)4 was a suitable method to recognise microsporum canis, microsporum gypseum, trichophyton rubrum and trichophyton interdigitale among 82 argentinian clinical isolates, producing the most simple and reproducible band profiles. in con ... | 2013 | 23412735 |
| formulation, characterization and evaluation of an optimized microemulsion formulation of griseofulvin for topical application. | the main objective of the study was to develop a microemulsion (me) formulation of griseofulvin for the treatment of dermatophytosis (indian patent application 208/del/2009). the oil phase was selected on the basis of drug solubility whereas the surfactant and cosurfactant were screened on the basis of their oil solubilizing capacity as well as their efficiency to form me from pseudo-ternary phase diagrams. the influence of surfactant and cosurfactant mass ratio (smix) on the me formation and it ... | 2013 | 23357739 |
| bioactive metabolites from guignardia sp., an endophytic fungus residing in undaria pinnatifida. | to isolate new and/or bioactive constituents from etoac extract of liquid culture of endophyte guignardia sp. from the leaves of undaria pinnatifida (harv.) sur. | 2012 | 23302536 |
| pcr test for microsporum canis identification. | microsporum canis, for which the natural hosts are cats and dogs, is the most prevalent zoophilic agent causing tinea capitis and tinea corporis in humans. we present here a diagnostic pcr test for m. canis, since its detection and species identification is relevant to the choice of treatment and to the understanding of a probable source of infection. an m. canis-specific pcr was evaluated using 130 clinical isolates of dermatophytes (including m. canis [n = 15] and 13 other species), 10 yeast o ... | 2013 | 23294424 |
| characterization and biological studies of bis- and tera-acetyl derivatives of hydrocarbon-bridged diamines-i. | a systematic study of the pharmaceutically important, double ended, chelating agents of the types ch(3)conh(ch(2))nnhcoch(3) and (ch(3)co)(2)n(ch(2))n n(coch(3))(2), where n= 2, 3, 4, 5 and 6, prepared by the bis- and tetra-acetylation of the corresponding diamino-polymethylenes, have been carried out. bis- and tertra-acetyl derivatives have been characterized by their elemental analysis and the ftir spectra, mass spectra and h-nmr spectra of these compounds have been reported to establish their ... | 2013 | 23261735 |
| a simple and rapid method to differentiate arthroderma benhamiae from microsporum canis. | arthroderma benhamiae is increasingly isolated in central europe. in culture, this dermatophyte is difficult to differentiate macroscopically from microsporum canis, which is microscopically also true for weak or non-sporulating strains. although there are valid molecular methods for differentiating between these two dermatophytes, in everyday practice it would be helpful for epidemiological and treatment considerations to have a simple and rapid method available for discrimination. | 2013 | 23194462 |
| disseminated dermatophytic pseudomycetoma caused by microsporum species. | dermatophyte infection is almost exclusively a superficial cutaneous mycosis usually confined to the stratum corneum of nails and hairs of normal hosts. deep cutaneous and subcutaneous infections due to dermatophytes are exceedingly rare and usually limited to immunosuppressed individuals. these infections remain chronic and persist in spite of treatment. | 2012 | 23171014 |
| feline polymorphonuclear neutrophils produce pro-inflammatory cytokines following exposure to microsporum canis. | the mechanisms involved in the establishment of the specific immune response against dermatophytes remain unknown. polymorphonuclear neutrophils (pmns) are recruited early during the infection process and participate in the elimination of dermatophytes. they could therefore be involved in the induction of the immune response during dermatophytoses by producing specific cytokines. the aim of this work was to assess the in vitro cytokine production by feline pmns exposed to living arthroconidia fr ... | 2013 | 23141297 |
| synthesis, antioxidant and antimicrobial activity of a new phloridzin derivative for dermo-cosmetic applications. | the phenolic compound phloridzin (phloretin 2′-o-glucoside, variously named phlorizin, phlorrhizin, phlorhizin or phlorizoside) is a prominent member of the chemical class of dihydrochalcones, which are phenylpropanoids. phloridzin is specifically found in apple and apple juice and known for its biological properties. in particular we were attracted by potential dermo-cosmetic applications. here we report the synthesis, stability studies and antimicrobial activity of compound f2, a new sem ... | 2012 | 23135632 |
| tinea corporis bullosa due to microsporum canis mimicking linear iga bullous dermatosis. | | 2012 | 23131347 |
| epidemiological trends in zoophilic and geophilic fungi in iran. | dermatophytes can be divided into geophilic (soil), zoophilic (animals) and anthropophilic (human) strains, depending on the source of the keratin. the predominant organisms vary within a given geographical region during different periods, which is influenced by a number of factors, such as population movements, socioeconomic circumstances and level of surveillance. thus, the incidence is very variable. | 2013 | 23095235 |
| in vitro activity of aminosterols against dermatophytes. | we recently reported that aminosterols are fungicidal due to their disrupting the outer membranes of yeasts and that they have a significant in vitro activity against various mould species. yet, their activity against dermatophytes had never been tested. this study's objective was to evaluate the in vitro activity of squalamine and a synthetic aminosterol derivative (asd) against various dermatophytes. susceptibility testing of squalamine, asd, terbinafine, and griseofulvin was performed, in tri ... | 2013 | 22998181 |
| comparative genome analysis of trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection. | the major cause of athlete's foot is trichophyton rubrum, a dermatophyte or fungal pathogen of human skin. to facilitate molecular analyses of the dermatophytes, we sequenced t. rubrum and four related species, trichophyton tonsurans, trichophyton equinum, microsporum canis, and microsporum gypseum. these species differ in host range, mating, and disease progression. the dermatophyte genomes are highly colinear yet contain gene family expansions not found in other human-associated fungi. dermato ... | 2012 | 22951933 |
| preparation and evaluation of antifungal efficacy of griseofulvin loaded deformable membrane vesicles in optimized guinea pig model of microsporum canis--dermatophytosis. | the present study is aimed at the encapsulation of griseofulvin in the deformable membrane vesicles (dmvs) for dermal delivery. presently, griseofulvin is available only in conventional oral dosage forms that suffer from the issues of poor and highly variable bioavailability, numerous systemic side effects and long duration of treatment. multi-lamellar drug-loaded dmvs of griseofulvin (indian patent application 208/del/2009) were prepared by thin-film hydration method and were optimized for type ... | 2012 | 22939964 |
| letter: tinea capitis in infants in their first 2 years of life: a 12-year study and a review of the literature. | tinea capitis (tc) is a dermatophyte infection that occurs mainly in childhood; but it is uncommon in infants. the aim of this study was to review the clinical and mycological profile of tc in infants. | 2012 | 22863638 |
| the microsporum canis genome is organized into five chromosomes based on evidence from electrophoretic karyotyping and chromosome end mapping. | the karyotype of microsporum canis was analyzed by contoured-clamped homogeneous electric field (chef) gel electrophoresis. four chromosomal bands that correspond to five chromosomes ranging from 3.0-6.2 mb were identified, adding the total genome size to approximately 24.9 mb. to confirm the number of chromosomes in m. canis, the number of telomeres was assessed by using a telomeric probe (ttaggg)(4) in southern blot analyses of digested genomic dna. treatment of m. canis dna with bal31 exonucl ... | 2013 | 22852750 |
| antifungal activity, toxicity and chemical composition of the essential oil of coriandrum sativum l. fruits. | the aims of this study were to test the antifungal activity, toxicity and chemical composition of essential oil from c. sativum l. fruits. the essential oil, obtained by hydro-distillation, was analyzed by gas chromatography/mass spectroscopy. linalool was the main constituent (58.22%). the oil was considered bioactive, showing an lc₅₀ value of 23 μg/ml in the artemia salina lethality test. the antifungal activity was evaluated against microsporum canis and candida spp. by the agar-well diffusio ... | 2012 | 22785271 |
| treatment outcomes for tinea capitis in a skin of color population. | tinea capitis periodically undergoes demographic shifts in causative dermatophyte and therapeutic response to oral anti-fungal therapy. | 2012 | 22777228 |
| subtilisin sub3 is involved in adherence of microsporum canis to human and animal epidermis. | the aim of this study was to assess the role of the secreted keratinolytic subtilisin-like protease sub3 in adherence of microsporum canis to epidermis from various susceptible species, in addition to cat for which this role was recently demonstrated. firstly, we showed by immunostaining that sub3 is not expressed in arthroconidia from an m. canis sub3 rna-silenced strain but is present on the surface of arthroconidia from a sub3 non-silenced parental strain. secondly, comparative adherence assa ... | 2012 | 22770520 |
| inflammatory tinea capitis: a 12-year study and a review of the literature. | inflammatory tinea capitis (tc) is a rare form of tc. the aim of this study was to review epidemiological, clinical and mycological profile of inflammatory tc. we present a retrospective study (1999-2010), enrolled all the cases of inflammatory tc observed at a referral hospital in the northern tunisia. one hundred and twenty-one patients with inflammatory tc, 83 male patients (68.6%) and 38 female patients (31.4%) were enrolled. the mean age was about 8 years. a majority of tc (71.9%) were in p ... | 2013 | 22757767 |
| preparation and characterization of liposomes containing essential oil of eucalyptus camaldulensis leaf. | the increased incidence of fungal resistance has necessitated the need to search for new antifungal agents. | 2012 | 24624167 |
| dermatophytosis and papular eosinophilic/mastocytic dermatitis (urticaria pigmentosa-like dermatitis) in three devon rex cats. | presenting signs: three devon rex cats were presented with multiple erythematous papules, occasionally associated with crusting and hyperpigmentation, with a linear distribution on the head, neck, chest and abdomen. one cat also had multifocal alopecia with hyperpigmentation on the dorsum. | 2012 | 22736683 |
| an improved molecular diagnostic assay for canine and feline dermatophytosis. | the few studies attempting to specifically characterize dermatophytes from hair samples of dogs and cats using pcr-based methodology relied on sequence-based analysis of selected genetic markers. the aim of the present investigation was to establish and evaluate a pcr-based approach employing genetic markers of nuclear dna for the specific detection of dermatophytes on such specimens. using 183 hair samples, we directly compared the test results of our one-step and nested-pcr assays with those b ... | 2013 | 22686247 |
| inhibition of the keratinolytic subtilisin protease sub3 from microsporum canis by its propeptide (prosub3) and evaluation of the capacity of prosub3 to inhibit fungal adherence to feline epidermis. | microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis, mainly in cats, dogs and humans. proteolytic enzymes have been postulated to be key factors involved in the invasion of the stratum corneum and keratinized epidermal structures. among these proteases, the secreted subtilisin protease sub3 was found to be required for adherence of m. canis arthroconidia to feline epidermis. this protease is synthetized as a preproenzyme consisting of a s ... | 2012 | 22633172 |
| extracellular production of silver nanoparticles by using three common species of dermatophytes: trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. | to develop a new green approach for biosynthesis of silver nanoparticles, myconanotechnology has been represented as a novel field of study in nanotechnology. in this study, we have reported the extracellular synthesis of highly stable silver nanoparticles using three species of dermatophytes: trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. | 2012 | 22562033 |
| preparation and cyclodextrin assisted dissolution rate enhancement of itraconazolium dinitrate salt. | the poor solubility of itraconazole (itr) results in its variable oral absorption and bioavailability and has also proven to be a major setback in developing an efficient oral delivery system. to improve its solubility and dissolution profile, itraconazolium dinitrate salt (itrdnt) was prepared and characterized using various spectral and thermal techniques. the morphology of the salt was studied using optical and scanning electron microscopy (sem). broth microdilution assay demonstrated antifun ... | 2013 | 22553909 |
| [cutaneous mycoses in japan originating from animals]. | human cases of dermatophytoses are occasionally transmitted from animals, and suffered from tinea corporis and sometimes kerion celsi. the most frequent causative agent of these diseases is microsporum canis. the other dermatophyte, arthroderma benhamiae is now prevailing in rabbits, rodents and hedgehogs that are popular household pets in japan. therefore, some human cases of a. benhamiae infection were reported and the transmission of this infection from rabbits and rodents was confirmed.crypt ... | 2012 | 22467127 |
| first case of subcutaneous dermatomycoses in a tunisian renal transplant patient. | dermatophytes are keratinophilic and usually infect the corneal layer of the epidermis and appendages on the occasion of immunosuppression, such as solid organ transplant, they can invade deeper tissues or cause an infection of the skin and subcutaneous disseminated. | 2012 | 22407643 |
| [frequency of dermatophytes in a sample of cats in the urban area of gran mendoza, argentina]. | the cat, considered the main reservoir of microsporum canis, lives in urban areas, and also plays an important role in the emergence of dermatomycoses. | 2012 | 22306766 |
| [epidemiology of tinea capitis in outpatients at the children's hospital in rabat (morocco)]. | the aims of this report on results of a retrospective study were to identify the incidence of this pathology among outpatients at the children's hospital in rabat, morocco, the species involved, their respective prevalence, and the influence of the age and sex of the patient. | 2011 | 24451494 |
| design, synthesis, and antifungal activity of new α-aminophosphonates. | α-aminophosphonates are bioisosteres of amino acids and have several pharmacological activities. these compounds have been synthesized by various routes from reaction between amine, aldehyde, and phosphite compounds. in order to synthesize α-aminophosphonates, catalytic effect of cucl2 was compared with fecl3. also all designed structures as well as griseofulvin were docked into the active site of microtubule (1jff), using autodock program. the results showed that the reactions were carried out ... | 2011 | 25954522 |
| alkylphenol activity against candida spp. and microsporum canis: a focus on the antifungal activity of thymol, eugenol and o-methyl derivatives. | in recent years there has been an increasing search for new antifungal compounds due to the side effects of conventional antifungal drugs and fungal resistance. the aims of this study were to test in vitro the activity of thymol, eugenol, estragole and anethole and some o-methyl-derivatives (methylthymol and methyleugenol) against candida spp. and microsporum canis. the broth microdilution method was used to determine the minimum inhibitory concentration (mic). the minimum fungicidal concentrati ... | 2011 | 25134762 |
| the use of ozonized oil in the treatment of dermatophitosis caused by microsporum canis in rabbits. | the ozone is effective against most microorganisms due to its high oxidant power. low concentrations and short-term contact are sufficient to inactivate bacteria, mold, yeast, parasites, seaweeds, protozoa and fungi. microsporum canis is an important agent of dermatophitosis in human and animal. the aim of the current study was to assess the efficacy of ozonized oil over microsporum canis in rabbits. eighteen male new zealand white rabbits, weight ranging from 2 to 3.2 kg were depilated in the c ... | 2011 | 24031632 |
| safety and efficacy of terbinafine in a pediatric iranian cohort of patients with tinea capitis. | tinea capitis is a common infection of the scalp and hair shaft caused by dermatophyte fungi that mainly affects prepubescent children. systemic therapy is required for treatment and to prevent spread. the aim of present study was to assess the effect of terbinafine for tinea capitis treatment in children. | 2009 | 23964167 |
| dermatophytosis caused by microsporum canis and microsporum gypseum in free-living bradypus variegatus (schiz, 1825) in the state of pernambuco, brazil. | three cases of dermatophytosis in free living brown-throated three-toed sloths (bradypus variegatus) in the zona da mata, north of pernambuco state, brazil, were studied. two animals presented areas of alopecia on the pelvic member and thorax and one animal on the pelvic member only. the three animals presented scabs. hair and scabs samples were submitted to microscopical examination after treatment with a 30 % koh and cultivated in mycosel agar. the direct examination indicated the presence of ... | 2008 | 24031255 |
| the development of the method for the determination of terbinafine in cat's plasma and hair. | clinical investigations of terbinafine indicate its high treatment activity against infections by several dermatophytes. its efficiency was tested also in the treatment of microsporosis in cats. the distribution of terbinafine in cat's plasma and hair is important for the identification of the drug efficiency. a fast and reliable reversed-phase high performance liquid chromatographic method with appropriate sample preparation has been developed.reliability, good reproducibility and low detection ... | 2000 | 28008526 |
| variation in microsporum canis and microsporum audouini. | | 1950 | 24538418 |
| composition and antifungal activity of zhumeria majdae essential oil. | essential oils extracted from different plants are extensively used in perfume, beverage, and food industries and are reported to exhibit antimicrobial activities against a variety of fungi. zhumeria majdae belonging to the lamiaceae family is a rare and endemic medicinal plant species in iran, with a strong and pleasant odor. the leaves of this plant have been used for many years as an antiseptic carminative agent for the treatment of stomachache (especially in infants) and dysmenorrhea. | 2015 | 28680999 |
| chemical composition, antibacterial and antifungal activities of crude dittrichia viscosa (l.) greuter leaf extracts. | the small amount of data regarding the antifungal activity of dittrichia viscosa (l.) greuter against dermatophytes, malassezia spp. and aspergillus spp., associated with the few comparative studies on the antimicrobial activity of methanolic, ethanolic, and butanolic extracts underpins the study herein presented. the total condensed tannin (tct), phenol (tpc), flavonoid (tfc), and caffeoylquinic acid (cqc) content of methanol, butanol, and ethanol (80% and 100%) extracts of d. viscosa were asse ... | 2017 | 28665341 |
| a study on etiologic agents and clinical manifestations of dermatophytosis in yazd, iran. | dermatophytosis is one of the most common infections of skin, hair, and nails, caused by a group of keratinophilic fungi known as dermatophytes. species identification of these fungi is of great significance from epidemiological and therapeutic points of view. the objective of the present study was to investigate dermatophytosis and its causative agents in patients, referring to the central mycology laboratory of yazd university of medical sciences, yazd, iran. | 2015 | 28681000 |
| tinea capitis by microsporum audouinii: case reports and review of published global literature 2000-2016. | tinea capitis caused by microsporum audouinii is reported herein from two brazilian schoolchildren, which are brothers. arthroconidia were evidenced on direct examination of scalp hair, and a fungus of the genus microsporum was isolated from cultures of each patient. the isolated fungi were classified as m. audouinii by visualization of species-specific structures, including: pectinate hyphae, chlamydospores, and fusiform macroconidia, sterile growth with characteristic brown pigment in rice gra ... | 2017 | 28736794 |
| [tinea capitis: main mycosis child. epidemiological study on 10years]. | despite the changes in their epidemiology, and the improving level of hygiene of the population, tinea capitis is still considered a public health problem in our country, and is the most common type of dermatophytosis in our country. the aim of our study was to evaluate the epidemiological, clinical and mycological features of tinea capitis in children encountered in the tunis region. a retrospective study concerned 1600 children aged 6 months to 15 years suspected to have tinea capitis was cond ... | 2017 | 28501468 |
| tinea capitis in children and trichoscopic criteria. | tinea capitis is the most common pediatric superficial dermatophyte infection. the causative species vary, as for instance, microsporum canis predominates in europe, while trichophyton tonsurans predominates in north america. tinea capitis does not respond well to topical therapy alone, thus oral therapy is requisite. the drug of choice is griseofulvin; however in some countries, it is no longer available. fungal culture should be requested in the persistent, scalp lesion and trichoscopy can put ... | 2017 | 28839385 |
| quantitative and structural analyses of the in vitro and ex vivo biofilm-forming ability of dermatophytes. | the aim of this study was to evaluate the in vitro and ex vivo biofilm-forming ability of dermatophytes on a nail fragment. | 2017 | 28708048 |
| the first case of onychomycosis in a koala (phascolarctos cinereus) due to atypical isolates of microsporum gypseum, a diagnostic challenge. | superficial mycotic infections have been only poorly described in koalas and there are no reliable mycologically confirmed data regarding clinical isolation of dermatophytes in this animal. we report an 11-year-old female koala, kept in a zoo in tokyo, japan, and presenting with hyperkeratotic lesions and scaly plaques on forepaw claws and pads reminiscent of fungal infection. | 2016 | 28681020 |
| molecular epidemiology of microsporum canis isolated in japan based on multilocus microsatellite typing fragment analysis. | intraspecies polymorphisms of microsporum canis strains isolated from human lesions and domesticated cats in japan were examined by multilocus microsatellite (ms) analysis. using nine ms markers, 70 strains were classified into 20 genotypes (genotypes a to t). of the nine ms markers, five were useful for genotyping, whereas the other four were not. the combination of ms2, ms4 and ms7 has the highest resolution power for inter-strain differentiation. genotype a, present in 15 strains (21.4%), was ... | 2017 | 28674311 |
| biofilm formation by microsporum canis. | | 2017 | 28642143 |
| epidemiological profile of tinea capitis in são paulo city. | tinea capitis is the most common fungal infection in children. the identification of the etiologic agent helps clinicians make their therapeutic choice. studies conducted in different countries show a changing pattern of the main etiological agents according to their regions. we performed a retrospective study in the tertiary public service in são paulo, analyzing the isolated etiological agents in patients with tinea capitis from march 2013 to may 2015. microsporum canis was the main agent (56. ... | 2017 | 28538903 |
| common dermatophytes and in vitro anti-fungal susceptibility testing in patients attending the dermatological clinic at the hospital for tropical medicine, bangkok. | dermatophytes comprising the genera trichophyton, microsporum, and epidermophyton are important causes of superficial mycoses. the number of infected patients and the distribution of species of these organisms in our hospital were unknown. we therefore aimed to investigate the clinical pattern of dermatophyte infections and to identify the species of these dermatophytes at the dermatological clinic of the hospital for tropical medicine, faculty of tropical medicine, mahidol university, bangkok i ... | 2017 | 28513812 |
| biosynthesis of zinc oxide nanoparticles by petals extract of rosa indica l., its formulation as nail paint and evaluation of antifungal activity against fungi causing onychomycosis. | aim: the authors report the biological synthesis of zinc oxide nanoparticles (zno-nps) from the petals extract of rosa indica l. (rose). its efficacy was evaluated against two dermatophytes: namely: trichophyton mentagrophytes and microsporum canis which cause onychomycosis. the activity of antibiotics against the tested dermatophytes was enhanced, when evaluated in combination with zno-nps. methods and results: the synthesised zno-nps were preliminary detected by using ultraviolet uv visible sp ... | 2017 | 28477005 |
| assessment of real-time pcr cycle threshold values in microsporum canis culture-positive and culture-negative cats in an animal shelter: a field study. | objectives real-time pcr provides quantitative information, recorded as the cycle threshold (ct) value, about the number of organisms detected in a diagnostic sample. the ct value correlates with the number of copies of the target organism in an inversely proportional and exponential relationship. the aim of the study was to determine whether ct values could be used to distinguish between culture-positive and culture-negative samples. methods this was a retrospective analysis of ct values from d ... | 2017 | 28425361 |
| a survey of the etiological agents of scalp and nail dermatophytosis in yazd, iran in 2014-2015. | tinea capitis and tinea unguium are regarded as global public health concerns. the purpose of the present study was to identify the etiological agents of tinea capitis and tinea unguium in patients, referring to the central laboratory of yazd university of medical sciences, yazd, iran. | 2015 | 28680997 |
| evaluation of zanthoxylum armatum roxb for in vitro biological activities. | zanthoxylum armatum fruits are used traditionally as a spice in various food preparations. the aim of this study was analysis of antimicrobial, cytotoxic, phytotoxic, insecticidal, and anti-leishmanial activity. the crude extract showed 86 ± 10% antifungal activity (agar tube dilution method) against trichophyton longifusis while n-hexane, chloroform, and aqueous-methanol fractions inhibited this pathogen by 90 ± 7, 85 ± 10 and 70 ± 9% respectively. the n-hexane and aqueous-methanol fraction als ... | 2017 | 29034201 |
| flavonoid composition and biological activities of ethanol extracts of caryocar coriaceum wittm., a native plant from caatinga biome. | caryocar coriaceum fruits, found in brazilian cerrado and caatinga, are commonly used as food and in folk medicine, as anti-inflammatory, bactericide, fungicide, leishmanicide, and nematicide. due to the biological potential of this plant, this study focuses on the evaluation of antifungal and antileishmanial activities, including anticholinesterase and antioxidant tests, correlating with total phenols and flavonoids content. peel extracts contain higher yield of phenols and flavonoids as analyz ... | 2017 | 29081821 |
| prp8 intein in dermatophytes: evolution and species identification. | dermatophytes are keratinophilic fungi belonging to the family arthrodermataceae. despite having a monophyletic origin, its systematics has always been complex and controversial. sequencing of nuclear ribosomal its and d1/d2 rdna has been proposed as an efficient tool for identifying species in this group of fungi, while multilocus analyses have been used for phylogenetic species recognition. however, the search for new markers, with sequence and size variation, which enable species identificati ... | 2017 | 29228309 |
| in vivoevaluation ofgalla chinensissolution in the topical treatment of dermatophytosis. | dermatophytosis is one of the main fungal diseases in humans and animals all over the world.galla chinensis, a traditional medicine, has various pharmacological effects. the goal of this study was to evaluate the treatment effect ofgalla chinensissolution (gcs) on dermatophytosis-infected dogs (microsporum canis,microsporum gypseum, andtrichophyton mentagrophytes, resp.). | 2017 | 29259645 |
| infection by microsporum canis in paediatric patients: a veterinary perspective. | microsporum canisis a dermatophyte fungus of which cats and dogs are recognized as the natural hosts.m. canisis also easily transmitted to humans, causing lesions to the glabrous skin (tinea corporis) and to the head (tinea capitis). the present study describes some cases of infection withm. canisin children from a veterinary perspective, highlighting some important features of this clinical entity (e.g., the necessity to identify the animal source of infection with appropriate diagnostic tests; ... | 2017 | 29056704 |
| an invertebrate infection model for evaluating anti-fungal agents against dermatophytosis. | animal models of pathogenic infection are needed to evaluate candidate compounds for the development of anti-infectious drugs. dermatophytes are pathogenic fungi that cause several infectious diseases. we established a silkworm dermatophyte infection model to evaluate anti-fungal drugs. injection of conidia of the dermatophyte arthroderma vanbreuseghemii into silkworms was lethal. a. vanbreuseghemii conidia germinated in liquid culture were more potent against silkworms than non-germinated conid ... | 2017 | 28947778 |
| evaluation of antifungal effect of silver nanoparticles againstmicrosporum canis,trichophyton mentagrophytesandmicrosporum gypseum. | dermatophytosis is the common cutaneous infections in humans and animals, which is caused by the keratinophylic fungus called dermatophytes. in recent years, drugs resistance in pathogenic fungi, including dermatophyte strains to the current antifungals have been increased. | 2015 | 28959308 |
| characterizing the clinical isolates of dermatophytes in hamadan city, central west of iran, using pcr-rlfp method. | dermatophytosis is one of the most common mycotic infections, which considered as a public health problem in the major of countries. this study evaluated the molecular epidemiology of dermatophytosis in patients referred to farshchian hospital in hamadan city with pcr-rflp method. | 2018 | 29439839 |
| activity of salvia dolomitica and salvia somalensis essential oils against bacteria, molds and yeasts. | essential oils (eos) fromsalvia dolomiticaandsalvia somalensis, widely employed in the cosmetic and perfume industry, were analyzed for composition and tested against bacterial and fungal pathogens isolated from clinical and environmental specimens. the analyses were carried out againststaphylococcus aureus,staphylococcus pseudointermedius,pseudomonas aeruginosa,escherichia coli,streptococcus canis,streptococcus pyogenes,klebsiella pneumoniae,proteus mirabilis,microsporum canis,microsporum gypse ... | 2018 | 29438274 |
| in vitro evaluation of new 2-phenoxy-benzo[g][1,2,4]triazolo[1,5-a]quinazoline derivatives as antimicrobial agents. | previously, seventeen 2-phenoxy-benzo[g][1,2,4]triazolo[1,5-a]quinazoline derivatives were prepared and characterized by physicochemical and spectral means. this study was conducted to evaluate their activities in vitro against five gram-negative and five gram-positive of clinically pathogenic bacterial strains and ten fungal strains. the antimicrobial activity was assessed, and the minimum inhibitory concentration values of the tested compounds were determined in μg ml-1, using the diffusion ag ... | 2018 | 29432912 |
| infant case of tinea faciei caused by microsporum canis. | | 2018 | 29411427 |
| correction to: resistance mechanism in a terbinafine-resistant strain of microsporum canis. | in the initial online publication, the name of author hock siew han was given incorrectly as han hock siew. the original article has been corrected. | 2018 | 29380187 |
| selection and validation of reference genes for qrt-pcr analysis of gene expression in microsporum canis growing under different adhesion-inducing conditions. | dermatophytes are the group of filamentous fungi infecting keratinized structures such as skin, hair, and nails. knowledge about genes and molecular mechanisms responsible for pathogenicity, as well as other biological properties of microsporum canis is still relatively poor. the qrt-pcr is a reliable technique for quantifying gene expression across various biological processes, and choosing a set of suitable reference genes to normalize the expression data is a crucial step of this technique. w ... | 2018 | 29352152 |
| resistance mechanism in a terbinafine-resistant strain of microsporum canis. | to clarify the terbinafine (trf) resistance mechanism in a trf-resistant strain of microsporum canis, the expression of the pleiotropic drug resistance (pdr1), multidrug resistance (mdr1), mdr2 and mdr4 genes were investigated by real-time quantitative pcr (rt-qpcr) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker fk506. the expression of the pdr1, mdr1, mdr2 and mdr4 genes was 2-4 times higher in the trf-resistant strain grown in t ... | 2018 | 29340910 |
| epidemiology of dermatophyte infections among school children in menoufia governorate, egypt. | most superficial mycotic infections of human skin are due to dermatophytes. children are frequently affected due to different predisposing factors, particularly overcrowding in classrooms. this study aimed to estimate the prevalence of dermatophytes infections and their related risk factors among school children in menoufia governorate, egypt. six public primary and preparatory schools were randomly selected and their pupils (n = 3464) were asked to complete a predesigned questionnaire covering ... | 2018 | 29314351 |
| epidemiological status of dermatophytosis in guilan, north of iran. | the epidemiological features of dermatophytoses have been characterized in many geographical locations of iran, but not in guilan, north of iran. this study was carried out to determine the distribution pattern of dermatophytoses and their relevant agents in guilan, north of iran, over a period of one year, from april 2010 to april 2011. | 2017 | 29302626 |
| in vitroantifungal activity of hexahydropyrimidine derivatives against the causative agents of dermatomycosis. | nitrogenated heterocyclic compounds are present in both natural and synthetic drugs, and hexahydropyrimidine derivatives may prove to be efficient in treating dermatomycosis causing fungi. this study evaluated the antifungal activity of four hexahydropyrimidine derivatives against the dermatomycosis causing fungi. these derivatives were synthesized, characterized, and assessed in terms of their activity againsttrichophyton mentagrophytes,microsporum canis,microsporum gypseum,trichophyton rubrum, ... | 2017 | 29226215 |
| antifungal activity of silver nanoparticles on fluconazole resistant dermatophytes identified by (gaca)4 and isolated from primary school children suffering from tinea capitis in ismailia - egypt. | fungal infections caused by dermatophytes recently became more common. available antifungal drugs are limited because of emergence of resistant strains due to prophylaxis with them, so there is an urgent need for novel antifungals. this study is aimed to detect the antifungal activity of silver nanoparticles (snps) on fluconazole resistant dermatophytes isolated from primary school children clinically suffering from tinea capitis and attending el-sheikh zaid dermatology center in ismailia. the s ... | 2017 | 29208175 |
| comparison of real-time pcr with fungal culture for the diagnosis of microsporum canis dermatophytosis in shelter cats: a field study. | objectives fungal culture requires at least 14 days for a final result, compared with 1-3 days for pcr. the study compared a commercial real-time dermatophyte pcr panel with fungal culture in cats in a shelter setting for: (1) diagnosis of microsporum canis infection; and (2) determination of mycological cure. methods this was a cross-sectional, observational study of cats with suspicious skin lesions or suspected exposure to dermatophytosis. hair samples were collected for fungal culture and pc ... | 2018 | 29172910 |
| genus- and species-level identification of dermatophyte fungi by surface-enhanced raman spectroscopy. | this paper demonstrates that surface-enhanced raman spectroscopy (sers) coupled with principal component analysis (pca) can serve as a fast and reliable technique for detection and identification of dermatophyte fungi at both genus and species level. dermatophyte infections are the most common mycotic diseases worldwide, affecting a quarter of the human population. currently, there is no optimal method for detection and identification of fungal diseases, as each has certain limitations. here, fo ... | 2018 | 29156315 |
| tinea folliculorum complicating tinea of the glabrous skin: an important yet neglected entity. | dermatophytes are capable of infecting the skin and its appendages such as nails and hairs producing a variety of clinical conditions. hair invasion by dermatophytes is a key feature of tinea capitis and tinea barbae but not of tinea of glabrous skin. in this project, we studied the clinico-mycological aspects of follicular involvement in patients with dermatophytosis of the glabrous skin. in total, 16 patients, eight males and eight females, were included in the study. all were adults except fo ... | 2017 | 29087496 |
| efficacy of itraconazole oral solution using an alternating-week pulse therapy regimen for treatment of cats with experimental microsporum canis infection. | objectives the objective of this study was to evaluate itraconazole 10 mg/ml oral solution for the treatment of microsporum canis infection using an alternating-week pulse therapy regimen in a controlled laboratory setting. methods eighty cats with experimentally induced infections were randomly assigned to treatment (itraconazole vs control [sterile water]), administered 5 mg/kg orally once daily for 1 week on alternate weeks for 5 weeks, followed by a 4 week follow-up period. topical therapeut ... | 2017 | 29025320 |
| proteolytic activity and cooperative hemolytic effect of dermatophytes with different species of bacteria. | globally, dermatophytes are the most common filamentous group of fungi causing cutaneous mycoses. dermatophytes were shown to secrete a multitude of enzymes that play a role in their pathogenesis. there is limited data on co-hemolytic (camp-like) effect of different bacterial species on dermatophyte species. in this study, we sought to the evaluate exoenzyme activity and co-hemolytic effect of four bacteria on clinical dermatophytes isolated from patients in shiraz, iran. | 2016 | 28959790 |
| evaluation of incubation time for microsporum canis dermatophyte cultures. | objectives the goal of this study was to determine how frequently microsporum canis was isolated after 1, 2 and 3 weeks of incubation on dermatophyte culture medium either from untreated cats or cats during treatment. methods this was an observational retrospective study. toothbrush fungal culture results were examined from two data pools: untreated cats with suspect skin lesions and weekly fungal cultures from cats being treated for dermatophytosis. results results from 13,772 fungal cultures w ... | 2017 | 28920535 |
| antimicrobial activity of essential oil and aqueous and ethanol extracts ofteucrium poliuml. subsp.gabesianum(l.h.) from tunisia. | the antimicrobial effects of essential oil, ethanol and aqueous extracts ofteucrium poliuml. were investigated against 13 microorganisms. extracts and essential oil were obtained from maceration, decoction and hydrodistillation respectively. samples were tested for their antimicrobial activity using the disk diffusion, the agar dilution and the agar incorporation method. essential oil was analysed using gc/ms, results showed that β-pinene (35.97%) and α-pinene (13.32%) were the main components. ... | 2017 | 28878510 |
| kerion celsi due to microsporum canis with a dermatophytid reaction. | microsporum (m.) canis is the most common fungus to cause tinea capitis in europe, especially in the mediterranean region and south and central europe. fungal scalp infections caused by m. canis tend to be non-inflammatory. recently, a growing number of cases of tinea capitis characterized by inflammatory infection caused by m. canis and m. gypseum have been registered. we present a case of highly inflammatory tinea capitis, also known as kerion celsi, caused by m. canis in a 6-year-old-patient. ... | 2017 | 28871931 |
| antifungal and antioxidant activities of organic and aqueous extracts of annona squamosa linn. leaves. | an increasing demand for natural additives has shifted the attention from synthetic to natural antioxidants and antifungal agents. this study was carried out to evaluate the antifungal and antioxidant activities of methanol, chloroform, and aqueous extracts of annona squamosa linn. leaves. the antifungal activities of all extracts of a. squamosa leaves against five different strains of fungi (alternaria alternata, candida albicans, fusarium solani, microsporum canis, and aspergillus niger) were ... | 2015 | 28911497 |