| direct detection of five common dermatophyte species in clinical samples using a rapid and sensitive 24-h pcr-elisa technique open to protocol transfer. | identification of dermatophytes is usually based on morphological characteristics determined by time-consuming microscopic and cultural examinations. an effective pcr-elisa method has been developed for rapid detection of dermatophyte species directly from clinical specimens within 24 h. isolated genomic dna of skin scrapings and nail samples from patients with suspected dermatophyte infections is amplified with species-specific digoxigenin-labelled primers targeting the topoisomerase ii gene. t ... | 2011 | 19793206 |
| antifungal activity of eugenia umbelliflora against dermatophytes. | antifungal activities of eugenia umbelliflora berg. (myrtaceae) were tested in vitro against a panel of standard and clinical isolates of human fungal pathogens (dermatophytes and opportunistic saprobes). methanol extracts of leaves and fruits of e. umbelliflora were separately prepared and partitioned, to yield dichloromethane (dcm), ethyl acetate (etoac) and aqueous fractions (aq). three compounds (1-3) were obtained from the dcm extract using chromatographic procedures. antifungal assays were ... | 2009 | 19831024 |
| majocchi's granuloma caused by microsporum canis in an immunocompetent patient. | | 2010 | 19832861 |
| prevalence of tinea capitis in southeastern austria between 1985 and 2008: up-to-date picture of the current situation. | tinea capitis is the most common dermatophyte infection in childhood, but may rarely occur in adults and the elderly. causative agents vary within different geographical areas as well as during decades. the aim of this study was to evaluate the prevalence and causative agents of tinea capitis in southeastern austria. retrospective analysis of 714 patients diagnosed with tinea capitis seen at the outpatient department of dermatology/medical university of graz during the time period 1985-2008 was ... | 2011 | 19863725 |
| [summarization of tinea capitis cases encountered at a clinic in the past 5 years]. | twelve cases of tinea capitis were encountered at a clinic in kumamoto prefecture between april 2004 and december 2008. patients were boys aged between 2 and 18 in 11 cases, and a woman aged 50 years in 1 case. the pathogen was microsporum canis in 6 cases, and trichophyton tonsurans in 6 cases. in the cases with m. canis , patients were boys aged 2 to 8 years in 5 cases, and the type of disease was non-inflammatory in 5 cases and inflammatory in 1 case. the patients with t. tonsurans were boys ... | 2009 | 19654449 |
| comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods. | the purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (mic) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. the percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, ... | 2009 | 19684970 |
| in vitro activity of tachyplesin iii alone and in combination with terbinafine against clinical isolates of dermatophytes. | aim of our study was to investigate the in vitro effects of tachyplesin iii (tp), a potent disulfide-linked peptide, in dermatophytes infections, with respect to or in combination with terbinafine (terb), against 20 clinical isolates of dermatophytes belonging to four species. a broth microdilution method following the clsi recommendations (m38-a) was used for testing drugs alone and in combination. terb mics were significantly lower than those observed for tp (p<0.001). testing for antifungal a ... | 2009 | 19591887 |
| comparison of the activities of four antifungal agents in an in vitro model of dermatophyte nail infection. | onychomycosis is a difficult condition to treat and cure rates are disappointing. moreover fungicidal action of antifungal agents in nccls assays and their rapid accumulation in nails in vivo are not compatible with the duration of treatment. | 2008 | 19882010 |
| evaluation of a pcr melting profile method for intraspecies differentiation of trichophyton rubrum and trichophyton interdigitale. | in order to identify the source of infections caused by dermatophytes, as well as the pathogen transmission pathway, there is a need to determine methods that allow detailed genetic differentiation of the strains within the dermatophyte genera. in this work, a pcr melting profile (pcr-mp) technique based on the ligation of adaptors and the difference in melting temperatures of dna restriction fragments was used for the first time for intraspecies genotyping of dermatophytes. clinical isolates an ... | 2010 | 19892858 |
| use of western blot to study microsporum canis antigenic proteins in canine dermatophytosis. | western blotting was used to describe the microsporum canis proteins with antigenic activity in dogs with dermatophytosis. electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. the proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 m. canis, 3 m. gypseum, 1 trichophyton mentagrophytes; group a), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no c ... | 2011 | 19912544 |
| the spectrum of fungal infections with a special reference to dermatophytoses in the capital area of kuwait during 2000-2005: a retrospective analysis. | fungal infections constitute a major health problem all over the world. signs and symptoms induced by various dermatophytic infections are difficult to distinguish clinically from each other. so, characterization by in vitro culture is required for appropriate diagnosis and treatment as well as to study the epidemiological characteristics in a region. | 2010 | 19921463 |
| organization and evolutionary trajectory of the mating type (mat) locus in dermatophyte and dimorphic fungal pathogens. | sexual reproduction in fungi is governed by a specialized genomic region, the mating type (mat) locus, whose gene identity, organization, and complexity are diverse. we identified the mat locus of five dermatophyte fungal pathogens (microsporum gypseum, microsporum canis, trichophyton equinum, trichophyton rubrum, and trichophyton tonsurans) and a dimorphic fungus, paracoccidioides brasiliensis, and performed phylogenetic analyses. the identified mat locus idiomorphs of m. gypseum control cell t ... | 2010 | 19880755 |
| tinea capitis in southeastern china: a 16-year survey. | this survey was a retrospective of a 16-year (1993-2008) study on the incidence, clinical features, and etiological agents of tinea capitis mainly representing the southeastern china. the diagnosis was confirmed by direct microscopic examination. eight hundred and sixty-six patients with tinea capitis, 381 males (44%) and 485 females (56%), were enrolled in this study. patients were between 20 days and 84 years old with an average of 10.5 years and the peak incidence was in the age group of 6-10 ... | 2010 | 19936963 |
| dynamics of dermatophytosis frequency in mexico: an analysis of 2084 cases. | we analysed 15,101 biological samples from patients presenting with superficial mycoses who attended outpatient services over a 10-year period. scale samples were processed for direct microscopic examination with 15% koh and cultured on sabouraud glucose agar plus chloramphenicol and cycloheximide. laboratory examination confirmed 4,709 cases of superficial mycosis (31.18%), of which 2,084 (44.26%) were dermatophytoses. the species most frequently encountered was trichophyton rubrum (71.2%), fol ... | 2010 | 19886762 |
| diagnostic pcr tests for microsporum audouinii, m. canis and trichophyton infections. | since traditional diagnosis of dermatophyte infections is slow, we present a rapid new pcr test for detection of trichophyton spp., microsporum canis and m. audouinii infections. the performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human dna control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected with m. canis; and two samples from un-infected control animals. dna ... | 2010 | 19886764 |
| a 5-year survey of dermatomycoses in southwest poland, years 2003-2007. | the study was performed to analyse the spectrum of dermatomycoses in southwest poland during the period 2003-2007. a total of 10,486 patients were investigated for fungal skin infections by means of native specimen and cultivating procedures. skin scrapings, plucked hairs and nail clippings were examined and identified by direct microscopy and culture. from 2468 patients, 2753 fungi were identified including dermatophytes, yeast and moulds. among the dermatophytes, the most common pathogen isola ... | 2011 | 19925570 |
| in vitro antimicrobial activity of four ficus carica latex fractions against resistant human pathogens (antimicrobial activity of ficus carica latex). | methanolic, hexanoïc, chloroformic and ethyl acetate extracts of ficus carica latex were investigated for their in vitro antimicrobial proprieties against five bacteria species and seven strains of fungi. the green fruit latex was collected from chott mariam souse, middle east coast of tunisia. the antimicrobial activity of the extracts was evaluated and based respectively on the inhibition zone using the disc-diffusion assay, minimal inhibition concentration (mic) for bacterial testing and the ... | 2010 | 20067867 |
| secreted subtilisin sub3 from microsporum canis is required for adherence to but not for invasion of the epidermis. | microsporum canis is a pathogenic dermatophyte that causes a superficial cutaneous mycosis, mainly in cats and humans. proteolytic enzymes, including subtilisins, have been postulated to be key factors involved in adherence and invasion of the stratum corneum and keratinized epidermal structures. | 2010 | 19995373 |
| identification of antibacterial and antifungal pharmacophore sites for potent bacteria and fungi inhibition: indolenyl sulfonamide derivatives. | synthesis of seven new indolenyl sulfonamides, have been prepared by the condensation reaction of indole-3-carboxaldehyde with different sulfonamides such as, sulphanilamide, sulfaguanidine, sulfathiazole, sulfamethoxazole, sulfisoxazole, sulfadiazine and sulfamethazine. these synthesized compounds have been used as potential ligands for complexation with some selective divalent transition metal ions (cobalt, copper, nickel & zinc). structure of the synthesized ligands has been deduced from thei ... | 2010 | 20005022 |
| synthesis, structure, and antifungal evaluation of some novel 1,2,4-triazolylmercaptoacetylthiosemicarbazide and 1,2,4-triazolylmercaptomethyl-1,3,4-thiadiazole analogs. | novel 1-[[4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-yl]mercaptoacetyl]-4-alkyl/aryl-3-thiosemicarbazides (5-12) were synthesized by the reaction of 4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-ylmercaptoacetylhydrazide (4) with substituted isothiocyanates. cyclodehydration of thiosemicarbazides with concentrated sulfuric acid yielded 2-[4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-yl]mercaptomethyl-5-alkyl/arylamino-1,3, 4-thiadiazoles (13-17). the new compounds were evaluated ... | 2010 | 20030516 |
| chemical constituents from the leaves of boehmeria rugulosa with antidiabetic and antimicrobial activities. | three new flavonoid glycosides, named chalcone-6'-hydroxy-2',3,4-trimethoxy-4'-o-beta-d-glucopyranoside (1), isoflavone-3',4',5,6-tetrahydroxy-7-o-[beta-d-glucopyranosyl-(1-->3)-alpha-l-rhamnopyranoside] (2), and isoflavone-3',4',5,6-tetrahydroxy-7-o-[beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->3)-alpha-l-rhamnopyranoside] (3), were isolated from the leaves of boehmeria rugulosa, together with five known compounds, beta-sitosterol, quercetin, 3,4-dimeth ... | 2009 | 20183275 |
| synthesis and antifungal activity of 1-aryl-3-phenethylamino-1-propanone hydrochlorides and 3-aroyl-4-aryl-1-phenethyl-4-piperidinols. | mono-mannich bases, 1-aryl-3-phenethylamino-1-propanone hydrochlorides, 1a, 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, and semi-cyclic mono-mannich bases, 3-aroyl-4-aryl-1-phenethyl-4-piperidinols, 1b, 2b, 3b, 4b, 5b, 6b, 7b, 8b, 9b, were synthesized by a non-classical mannich reaction. the aryl part was: c(6)h(5 )for 1a, 1b; 4-ch(3)c(6)h(4) for 2a, 2b; 4-ch(3)oc(6)h(4 )for 3a, 3b; 4-clc(6)h(4 )for 4a, 4b; 4-fc(6)h(4) for 5a, 5b; 4-brc(6)h(4) for 6a, 6b; 2,4-(cl)(2)c(6)h(3) for 7a, 7b; 4-no(2)c(6)h(4 )for ... | 2010 | 20232370 |
| steroidal glycosides from the underground parts of allium ursinum l. and their cytostatic and antimicrobial activity. | the aim of this study was the isolation and structural elucidation of steroidal glycosides from the underground parts of ramson allium ursinum l. the structures of the isolated compounds were established based upon chromatographic methods and 1d- and 2d-nmr, ms and ir analyses. the mixture of two steroidal saponins: (25r)-spirost-5-en-3b-ol tetrasaccharide and (25r)-spirost-5, 25(27)-dien-3b-ol tetrasaccharide, along with a 3-hydroxypregna-5,16-dien-20-one glycoside were identified. the results ... | 2006 | 20085228 |
| cutaneous mycoflora and cd4:cd8 ratio of cats infected with feline immunodeficiency virus. | this study was designed to compare cutaneous mycoflora isolation and cd4+:cd8+ ratio in feline immunodeficiency virus (fiv)-infected cats with that in fiv-uninfected cats. sixty cats were examined. twenty-five were fiv-infected cats and 35 were fiv-uninfected cats. all 60 cats were felv-negative. fungi were speciated and immunophenotyping of peripheral cd4+ and cd8+ t lymphocytes was performed. at least one fungal colony was isolated from 22/25 (88%) fiv-infected cats. among the fiv-uninfected c ... | 2010 | 20156698 |
| recovery of dermatophytes in pet grooming tools from veterinary clinics and pet grooming salons. | fomites such as brushes and clippers are known to be potential risk factors for the transmission of dermatophytes between pet animals. the aim of this study was to investigate this risk by examining those grooming implements for the presence of any viable dermatophytes. | 2010 | 20137008 |
| synthesis and antileishmanial and antimicrobial activities of some 2,3-disubstituted 3h-quinazolin-4-ones. | a series of 2,3-disubstituted 3h-quinazolin-4-ones was synthesized. antimicrobial activities of the synthesized compounds were investigated against gram (+ve) and gram (-ve) bacteria, including b. subtilis, s. aureus, s. flexneri, p. aeruginosa, and s. typhi, and six fungi, namely trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani, and candida glabrata using the broth microdilution method. compounds 9, 11, and 12 showed significant activities agains ... | 2010 | 20235747 |
| a zoonotic ringworm outbreak caused by a dysgonic strain of microsporum canis from stray cats. | cats are frequent carriers of microsporum canis and veterinary students are at high risk of exposure and acquisition of the organism a la infección. | 2010 | 20346301 |
| inflammatory tinea capitis: kerion, dermatophytic granuloma, and mycetoma. | inflammatory tinea capitis is the result of a hypersensitivity reaction to a dermatophytic infection. the usual forms are favus, kerion celsi, dermatophytic majocchi granuloma, and mycetoma. inflammatory tinea capitis can be caused by microsporum canis, trichophyton mentagrophytes, t tonsurans, t rubrum, and m gypseum. histopathologic findings include a spectrum from mild suppurative folliculitis to dense granulomatous infiltrates. in mycetoma, grains must be present. | 2010 | 20347653 |
| extraction, separation and isolation of volatiles from vitex agnus-castus l. (verbenaceae) wild species of sardinia, italy, by supercritical co2. | isolation of volatile concentrates from leaves, flowers and fruits of vitex agnus-castus l. have been obtained by supercritical extraction with carbon dioxide. the composition of the volatile concentrates has been analysed by gc/ms. in all plant organs, the extracts are composed chiefly of alpha-pinene, sabinene, 1,8-cineole, alpha-terpinyl acetate, (e)-caryophyllene, (e)-beta-farnesene, bicyclogermacrene, spathulenol and manool. the main difference observed was in the content of sclarene, which ... | 2010 | 20397107 |
| [dermatophytoses due to anthropophilic fungi in cadiz, spain, between 1997 and 2008]. | cutaneous fungal infections are a major public health problem. the distribution of the dermatophytoses varies between countries and geographical areas. the aim of this study was to determine the incidence, epidemiology, etiology, and clinical course of the dermatophytoses caused by anthropophilic fungi in cadiz, spain, over the past 12 years. | 2010 | 20398600 |
| majocchi granuloma caused by microsporum canis as tinea incognito. | | 2010 | 20406391 |
| favus of the scalp: an overview and update. | tinea capitis favosa, a chronic inflammatory dermatophyte infection of the scalp, affects over 90% of patients with anthropophilic trichophyton schoenleinii. t. violaceum, t. verrucosum, zoophilic t. mentagrophytes (referred to as 'var. quinckeanum'), microsporum canis, and geophilic m. gypseum have also been recovered from favic lesions. favus is typically a childhood disease, yet adult cases are not uncommon. interestingly, favus is less contagious than other dermatophytoses, although intrafam ... | 2010 | 20411336 |
| epidemiology and risk factors for dermatophytoses in rabbit farms. | while dermatophytoses of several animal species have been extensively investigated, information on their occurrence and epidemiology in rabbits is limited. we carried out a study from october 2006 to february 2007 of 23 rabbit farms in apulia and basilicata regions (southern italy) in order to investigate the occurrence and risk factors associated with dermatophytoses in breeding rabbits. dermatophytes were isolated from 86.9% (20/23) of the farms and from 51.8% (420/810) of the animals sampled. ... | 2010 | 20297949 |
| [tinea capitis: two years experience in a paediatric hospital of buenos aires, argentina]. | tinea capitis is an infection caused by dermatophytes of the genera microsporum and trichophyton, and constitutes a major health problem in argentina. the aim of the present study was to find out the incidence of those etiological agents and the therapeutic response in patients attending a high-complexity paediatric hospital within a two-year period. a total of 98 tinea capitis were diagnosed, 13 of which were celsus kerion. microsporum canis was isolated in 61.28%. the range of values for minim ... | 2010 | 20346290 |
| disease progression of dermatophytic pseudomycetoma in a persian cat. | dermatophytic pseudomycetoma is a subcutaneous fungal infection by microsporum canis. | 2010 | 20346294 |
| tinea capitis in adults in tunisia. | objective: to determine the pattern of infectious agents causing tinea capitis (tc) in adult patients in the center of tunisia. methods: from january 1990 to december 2005, we have retrospectively collected all cases of adult tc, confirmed by the mycological examination. results: sixty patients (18 male, 42 female) with a mean age of 34.5 years were diagnosed as having adult tc among a total number of 1137 cases of tc (5.27%). clinical features were polymorphic and diagnosis was made on mycologi ... | 2010 | 20534084 |
| a 30-year survey of paediatric tinea capitis in southern spain. | tinea capitis (tc) is the most common mycological infection in childhood. the epidemiology of tc varies depending on the geographical area and the time of study, and although it is an important public health problem in our area, only a few long-term studies have focused on children. | 2011 | 20553362 |
| [emergence of microsporum audouinii and trichophyton tonsurans as causative organisms of tinea capitis in the dominican republic]. | tinea capitis affects mainly children. the frequency and causative organism vary from region to region. | 2010 | 20487688 |
| fungalysin and dipeptidyl-peptidase gene transcription in microsporum canis strains isolated from symptomatic and asymptomatic cats. | microsporum canis is the main pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis in domestic carnivores. in cats, m. canis causes symptomatic or asymptomatic infection. recent conflicting data raise the question of whether the clinical status of the infected cat (symptomatic or asymptomatic) is directly correlated to the proteolytic activity of m. canis strains. here, the transcription of fungalysin and dipeptidyl-peptidase genes (dpp) of m. canis was compared ... | 2010 | 20488631 |
| in vivo antifungal effect of combretum and terminalia species extracts on cutaneous wound healing in immunosuppressed rats. | acetone leaf extracts of combretaceae species combretum imberbe wawra, combretum nelsonii duemmer, combretum albopunctatum suesseng, and terminalia sericea burch ex dc and a mixture of asiatic acid and arjunolic acid isolated from c. nelsonii were tested for antifungal activity against candida albicans, cryptococcus neoformans, microsporum canis, and sporothrix schenckii on wounds of immunocompromised wistar rats. the therapeutic agents were selected based on low mic values ranging 0.02-2.5 mg/m ... | 2010 | 20645734 |
| microsporum canis antifungal susceptibilities: concerns regarding their clinical predictability. | | 2010 | 20685087 |
| development of efficient tools for genetic manipulation of dermatophytes. | molecular biological approaches have recently begun to be applied to molecular genetics studies of dermatophytes. high-throughput gene analysis methodologies, such as est sequencing, differential cdna screening, and cdna-based microarray analysis have been used to obtain information on many dermatophyte genes and their expression profiles under different experimental conditions. in addition, whole genome sequencing projects are underway for several important dermatophytes, such as trichophyton r ... | 2010 | 20467196 |
| evaluation of the association of superficial dermatophytosis and athletic activities with special reference to its prevention and control. | this study was conducted to evaluate the association of superficial mycosis and athletic activities with special references to its prevention and control in tehran. participating in various kinds of sports can lead to direct and indirect exposures to and transmission of micro-organisms between athletes and also passive observers. | 2010 | 20636349 |
| use of itraconazole and either lime sulphur or malaseb concentrate rinse ® to treat shelter cats naturally infected with microsporum canis: an open field trial. | in an open non-randomized study, 90 cats with severe dermatophytosis were treated with 21 days of oral itraconazole at 10 mg/kg and one of three topical antifungal rinses applied twice weekly: lime sulphur (lso); reformulated lime sulphur with an odour-masking agent (lsr); or a 0.2% miconazole nitrate and 0.2% chlorhexidine gluconate rinse (mc). weekly examinations and fungal cultures were used to monitor the cats' response to therapy. if at day 42 of treatment cats were still strongly fungal cu ... | 2011 | 20604908 |
| dermatophytes in republic of macedonia. | (full text is available at http://www.manu.edu.mk/prilozi). a total of 1742 clinically suspected samples was examined for the incidence and distribution of dermatophytes in the republic of macedonia from june 2007 to jan. 2009. 600 dermatophytes were isolated and identified. in the studied period 9 different dermatophyte species were isolated, namely trichophyton rubrum (48.83%), microsporum canis (20%), trichophyton mentagrophytes var. interdigitale (16.83%), epidermophyton floccosum (4.17%), t ... | 2010 | 20693949 |
| chemical composition and biological assays of essential oils of calamintha nepeta (l.) savi subsp. nepeta (lamiaceae). | aerial parts of wild calamintha nepeta (l.) savi subsp. nepeta growing spontaneously on the mediterranean coast (sardinia island, italy) and on the atlantic coast (portugal) were used as a matrix for the supercritical extraction of volatile oil with co(2). the collected extracts were analysed by gc-fid and gc-ms methods and their compositions were compared with that of the essential oil isolated by hydrodistillation, but the differences were not relevant. a strong chemical variability was observ ... | 2010 | 20981614 |
| efficacy of eight commercial formulations of lime sulphur on in vitro growth inhibition of microsporum canis. | lime sulphur is a common topical treatment for dermatophytosis in animals. until recently, a single veterinary lime sulphur formulation was available. the purpose of this study was to compare the efficacy of eight lime sulphur products for in vitro growth inhibition of microsporum canis using the isolated infected spore model. infective m. canis spores were isolated from hairs collected from untreated cats. hairs were macerated in triton-x solution and isolated according to a previously publishe ... | 2010 | 20868396 |
| effects of temperature variations and light exposure on the time to growth of dermatophytes using six different fungal culture media inoculated with laboratory strains and samples obtained from infected cats. | in this study, 5/6 commercially available fungal culture media were comparable with respect to first growth, first colour change, and first sporulation when inoculated with three strains of microsporum canis, one strain of microsporum gypseum, and one strain of trichophyton species when incubated at either 25°c or 30°c. five of six plates showed 100% growth at both temperatures. five of six plates showed 100% growth when inoculated with naturally infective m canis hairs and spores. one commercia ... | 2010 | 21051255 |
| in vitro profiling of pramiconazole and in vivo evaluation in microsporum canis dermatitis and candida albicans vaginitis laboratory models. | the triazole antifungal pramiconazole (stiefel, a gsk company) was compared with itraconazole, miconazole, and terbinafine in vitro and in vivo. potent in vitro activities against candida spp. (50% inhibitory concentration [ic₅₀], 0.04 to 1.83 μm) and microsporum and trichophyton spp. (ic₅₀, 0.15 to 1.34 μm) were obtained but not, however, against other filamentous molds and zygomycetes. in the m. canis guinea pig model and c. albicans vulvovaginitis rat model, pramiconazole was superior to the ... | 2010 | 20805398 |
| the spectrum of dermatological disorders among primary school children in dar es salaam. | dermatologic disorders are common in many countries but the spectrum varies greatly. many studies have reported a significant burden of skin diseases in school children. the objective of this study was to determine the current spectrum of dermatological disorders in primary school children in dar es salaam city. | 2010 | 21162714 |
| detection of antimicrobial compounds by bioautography of different extracts of leaves of selected south african tree species. | the hexane, acetone, dichloromethane and methanol extracts of combretum vendae a.e. van wyk (combretaceae), commiphora harveyi (engl.) engl. (burseraceae), khaya anthotheca (welm.) c.dc (meliaceae), kirkia wilmsii engl. (kirkiaceae), loxostylis alata a. spreng. ex rchb. (anacardiaceae), ochna natalitia (meisn.) walp. (ochnaceae) and protorhus longifolia (bernh. ex c. krauss) engl. (anacardiaceae) were screened for their antimicrobial activity. the test organisms included bacteria (enterococcus f ... | 2009 | 21304615 |
| changes of dermatophytoses in southwestern greece: an 18-year survey. | the isolation and distribution rate of dermatophytes as causative agents of superficial mycoses of skin, hair, and nails during an 18-year period (1991-2008) at a university hospital are presented. a comparative analysis of epidemiological differences within the first (1991-1999) and the second 9-year period (2000-2008) was performed. skin scrapings, nail, and hair specimens were examined by a direct microscopic examination and culture. identification of dermatophyte species was based on macrosc ... | 2011 | 21331751 |
| clinico-epidemiological and mycological aspects of tinea incognito in iran: a 16-year study. | tinea incognito is a dermatophytic infection in which topical or systemic steroids have modified the clinical appearance of the mycosis and mimicking other skin diseases. | 2011 | 21441710 |
| geotrichum candidum as etiological agent of horse dermatomycosis. | fungi of the genus geotrichum are commonly found in the environment and, in some circumstances, they may cause diseases in humans and animals. although these fungi have been isolated from skin lesions of some animal species, their pathogenic role in horses remains uncertain. with the aim to investigate the role of geotrichum candidum as etiological agent of dermatomycoses, a retrospective study of 64 horses presenting skin lesions and suspected to have fungal infections was carried out. for each ... | 2010 | 20970928 |
| mycetoma of the scalp due to microsporum canis: hystopathologic, mycologic, and immunogenetic features in a 6-year-old girl. | dermatophytic mycetoma is an extremely rare subcutaneous mycosis. here, we report the case of a 6-year-old girl with clinical, histologic, and mycologic findings consistent with a mycetoma of the scalp caused by microsporum canis. to our knowledge, this is the first report showing the immunologic and immunogenetic features of a patient with a recalcitrant dermatophytic mycetoma. | 2011 | 21513850 |
| isolation of dermatophytes from dogs and cats with suspected dermatophytosis in western turkey. | the aim of this study was to determine the species of dermatophytes isolated from dogs and cats and their prevalence in the two big provinces of western turkey. a total of 362 animals (198 dogs and 164 cats) with skin lesions (alopecia and desquamation) were examined from march 2006 to february 2008. of the 362 samples examined, 52 (14.4%) were positive for fungal elements by direct microscopic examination, and 70 (19.3%) were culture positive for dermatophytes. the isolation rates of dermatophy ... | 2010 | 21126787 |
| cluster analysis of microsporum canis isolated from a patient with tinea corporis and an infected cat based on the dna sequences of nuclear ribosomal internal transcribed spacer 1. | | 2011 | 21615535 |
| composition of a volatile extract of eryngium duriaei subsp. juresianum (m. laínz) m. laínz, signalised by the antifungal activity. | the composition of a volatile extract of eryngium duriaei subsp. juresianum, signalised by the antifungal activity (mic values=0.16-0.32 μl ml(-1)) against several dermatophyte species (trichophyton mentagrophytes, t. rubrum, epidermophyton floccosum; t. verrucosum, t. mentagrophytes var interdigitale, microsporum canis and m. gypseum) was established following a combined methodology of gc, gc-ms and an exclusive (13)c nmr technique that does not require prior isolation of compounds. twenty-five ... | 2010 | 21036502 |
| neonatal dermatophytosis: report of a case and review of the literature. | tinea capitis is a common fungal infection in children but rare among neonates. we present a case of a 3-week-old infant with multiple scalp lesions for 1 week. a diagnosis of tinea capitis due to microsporum canis was made based on clinical morphology, wood's lamp, and biopsy with a positive pas stain. she was effectively treated with oral griseofulvin and ketoconazole shampoo. | 2011 | 21504448 |
| synthesis, characterization and biological studies of sulfonamide schiff's bases and some of their metal derivatives. | a new series of schiff base ligands derived from sulfonamide and their metal(ii) complexes [cobalt(ii), copper(ii), nickel(ii) and zinc(ii)] have been synthesized and characterized. the nature of bonding and structure of all the synthesized compounds has been explored by physical, analytical and spectral data of the ligands and their metal(ii) complexes. the authors suggest that all the prepared complexes possess an octahedral geometry. the ligands and metal(ii) complexes have been screened for ... | 2011 | 21534864 |
| mechanisms of skin adherence and invasion by dermatophytes. | dermatophytes are keratinophilic fungi that can be pathogenic for humans and animals by infecting the stratum corneum, nails, claws or hair. the first infection step consists of adherence of arthroconidia to the stratum corneum. the mechanisms and the kinetics of adherence have been investigated using different in vitro and ex vivo experimental models, most notably showing the role of a secreted serine protease from microsporum canis in fungal adherence to feline corneocytes. after germination o ... | 2011 | 21831104 |
| ganoderma lucidum: a source for novel bioactive lectin. | ganoderma lucidum is known for its high medicinal value, clinically used in treatment for various diseases. we have selected this mushroom for isolation of novel bioactive lectin. the isolation procedure comprised of ion-exchange chromatography on deae- cellulose and affinity chromatography on affi-gel blue gel. purified lectin was monomer with a molecular mass of 15 kda, determined by sds-page, gel filtration, maldi-tof. it showed hemagglutinating activity against both human and animal erythroc ... | 2011 | 21728991 |
| Zoonoses of procyonids and nondomestic felids. | There are several important zoonotic diseases which can be acquired from procyonids, and nondomestic felids. Baylisascaris procyonis, the raccoon roundworm, is a common parasite of raccoons and can cause visceral, ocular, or neural larval migrans in people. Neural larval migrans can cause severe signs in individuals. Dermatophytosis and enteric pathogens are the most important zoonotic agents found in nondomestic felids. Microsporum canis infections can be spread from nondomestic felids to owner ... | 2011 | 21872788 |
| strains differentiation of microsporum canis by rapd analysis using (gaca)4 and (aca)5 primers. | molecular analysis of dermatophytes (based on pcr fingerprinting) revealed high clonal differentiation between the genus and species. microsporum canis (zoophilic dermatophyte, belonging to genus microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. results of most molecular studies show that there is no clonal differentiation within m. canis as distinct from other specie ... | 2011 | 21905632 |
| molecular identification and phylogenesis of dermatophytes isolated from rabbit farms and rabbit farm workers. | little information is available on the molecular epidemiology of dermatophytoses in rabbit farms and farm workers. a total of 117 isolates belonging to the trichophyton mentagrophytes complex and 21 isolates of microsporum canis were collected from rabbits with or without skin lesions, air samples of farms known to harbour these pathogens, and from farm workers with skin lesions, and molecularly characterized. sequencing of amplicons from the t. mentagrophytes complex and m. canis isolates revea ... | 2011 | 21840652 |
| Difference in keratinase activity of dermatophytes at different environmental conditions is an attribute of adaptation to parasitism. | Dermatophytes are a group of morphologically and physiologically related moulds, which cause well-defined infection called dermatophytosis. The enzymatic ability of fungi to decompose keratin has long been interpreted as a key innovation in the evolution of animal dermatology. In the present study, keratinase activity profile among Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis and Microsporum gypseum isolated on keratin substrates such as human hair, human nail and chicken ... | 2011 | 22032519 |
| analysis of 25 cases of microsporum canis infection encountered at a dermatology clinic in kumamoto during a recent 3-year period. | twenty-five cases of dermatophytoses caused by microsporum canis were encountered during a 3-year period (january 2008-december 2010). their diagnosis was based on detection of fungal elements by direct microscopy and identification of m. canis by fungal culture. there were 17 women and 8 men ; they ranged in age from 4 to 85 years (mean, 34.6). the affected site was the head (n=5), face (n=8), neck (n=5), arm (n=8), leg (n=5), and trunk (n=5) ; exposed sites were those most often affected. a lo ... | 2011 | 21788725 |
| The dog mite, Demodex canis: prevalence, fungal co-infection, reactions to light, and hair follicle apoptosis. | Infection rate, reaction to light, and hair follicle apoptosis are examined in the dogmite, Demodex canis Leydig (Prostigmata: Demodicidae), in dogs from the northern area of Taiwan. An analysis of relevant samples revealed 7.2% (73/1013) prevalence of D. canis infection. Infection during the investigation peaked each winter, with an average prevalence of 12.5% (32/255). The infection rates significantly varied in accordance with month, sex, age, and breed (p < 0.05). Most of the lesions were di ... | 2011 | 21867442 |
| isolation of the volatile oil from satureja thymbra by supercritical carbon dioxide extraction: chemical composition and biological activity. | satureja thymbra l. is well known in italy by the popular name of "santoreggia sarda". it grows only in sardinia and nowadays it is restricted to the slope of the colle san michele in cagliari. the composition of the aromatic extracts obtained by supercritical co2 and by hydrodistillation and their antifungal activity is reported. the collected extracts were analyzed by gc-fid and gc-ms methods. no significant differences were observed in the composition of the volatile extracts depending on the ... | 2011 | 22164799 |
| microsporum canis tinea capitis in a centenarian patient. | | 2011 | 21860180 |
| Interkingdom Gene Transfer of a Hybrid NPS/PKS from Bacteria to Filamentous Ascomycota. | Nonribosomal peptides (NRPs) and polyketides (PKs) are ecologically important secondary metabolites produced by bacteria and fungi using multidomain enzymes called nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs), respectively. Previous phylogenetic analyses of fungal NRPSs and PKSs have suggested that a few of these genes were acquired by fungi via horizontal gene transfer (HGT) from bacteria, including a hybrid NPS/PKS found in Cochliobolus heterostrophus (Dothideomycet ... | 2011 | 22140558 |
| Chemical composition and biological activity of the volatile extracts of Achillea millefolium. | In this study, flowering aerial parts of wild Achillea millefolium growing on the Mediterranean coast (Sardinia Island, Italy) and on the Atlantic coast (Portugal- Serra de Montemuro) were used as a matrix for supercritical extraction of volatile oil with CO2 (SFE). The collected extracts were analyzed by GC-FID and GC-MS methods and their composition were compared with that of the essential oil isolated by hydrodistillation. A strong chemical variability in essential oils depending on the origi ... | 2011 | 22164800 |
| [scalp ringworm tinea capitis in tunisian infants.] | objective: the aim of the study was to specify the epidemiological, clinical, and mycological particularities of tinea capitis in infants. design: we retrospectively collected data from the files of 245 infants presenting with tinea capitis, followed in the hedi-chaker hospital dermatology department and in two mycology laboratories of the sfax hospital, between january 1995 and december 2006. we collected the epidemiological, clinical, and mycological data for each patient. results: we included ... | 2011 | 21764534 |
| preparation and evaluation of dermal delivery system of griseofulvin containing vitamin e-tpgs as penetration enhancer. | griseofulvin, an antifungal agent, is a bcs class ii drug slowly, erratically, and incompletely absorbed from the gastrointestinal tract in humans. the clinical failure of the conventional oral therapy of griseofulvin is most likely attributed to its poor solubility and appreciable inter- and intra-subject variation in bioavailability from different commercial products. moreover, the conventional oral therapy is associated with numerous adverse effects and interactions with other drugs. the purp ... | 2011 | 22130790 |
| tinea faciei due to microsporum canis in children: a survey of 46 cases in the district of cagliari (italy). | dermatophytoses are frequent in children, but involvement of the facial skin has peculiar aspects that should be considered a separate entity: tinea faciei. microsporum canis infection in tinea faciei has not been widely documented. to review cases of tinea faciei due to m. canis in children diagnosed at the dermatology clinic, university of cagliari. between 1990 and 2009, all children with dermatophyte infections of the facial skin were recruited for the study after parental consent. diagnosis ... | 2011 | 22011084 |
| [superficial mycoses in the city of valparaiso, chile: period 2007-2009]. | superficial mycoses are infections of the keratinous tissue caused by dermatophytes, yeasts and environmental fungi. | 2011 | 22051613 |
| analysis of the differentially expressed genes in microsporum canis in inducing smooth skin and scalp tissue conditions. | microsporum canis is a common zoophilic dermatophyte, which causes a range of infections. to explore the pathogenic mechanism of tinea capitis, we used the suppression subtractive hybridization (ssh) technique to investigate the differences in gene expression between different cultures of microsporum canis incubated on three different types of mineral media containing child glabrous skin, child scalp tissue and adult scalp tissue. using dot-blot hybridization and real-time pcr technique, we succ ... | 2011 | 21883399 |
| Antifungal activity and chemical composition of essential oils from Smyrnium olusatrum L. (Apiaceae) from Italy and Portugal. | The essential oils and supercritical CO(2) extracts of wild Smyrnium olusatrum L. growing in Sardinia (Italy) and in Portugal were investigated. For the study, oils were isolated from total plant aerial part (umbels containing seeds). The content of ß-phellandrene (67.3% vs. 42.7%) and a-pinene (31.9% vs. 1.2%), respectively, the main components of Portuguese and Italian essential oils, declined during the maturation stage of the umbels. Contrarily, some other important components, particularly ... | 2011 | 21902563 |
| Decreasing Prevalence of Microsporum canis Infection in Korea: Through Analysis of 944 Cases (1993-2009) and Review of Our Previous Data (1975-1992). | Since 1957, Microsporum (M.) canis has been one of the common causative agents of dermatophytosis in Korea. We analyzed 944 patients infected with M. canis who attended outpatient clinic over a 17-year period (1993-2009). M. canis infections were diagnosed by mycological examinations, including direct microscopic examinations with 15% KOH and cultures on potato dextrose agar complemented with 0.5% chloramphenicol. Mycological examinations confirmed 164,903 cases of dermatophytosis, 944 (0.6%) of ... | 2011 | 22187358 |
| design, synthesis and antifungal activity of some new imidazole and triazole derivatives. | triazole and imidazole are incorporated into the structures of many antifungal compounds. in this study a novel series of 1,2,4-triazole, imidazole, benzoimidazole, and benzotriazole derivatives was designed as inhibitors of cytochrome p450 14α-demethylase (14dm). these structures were docked into the active site of mt-cyp51, using autodock program. sixteen compounds with the best binding energy were synthesized. the chemical structures of the new compounds were confirmed by elemental and spectr ... | 2011 | 21984016 |
| multilocus differentiation of the related dermatophytes microsporum canis, microsporum ferrugineum and microsporum audouinii. | microsporum ferrugineum, an uncommon causative agent of dermatophytosis, has restricted endemicity. iranian strains suspected to be m. ferrugineum from two patients with tinea were analysed using the rdna internal transcribed spacer (its) region and the partial β-tubulin (bt2) and translation elongation factor 1-α (tef1) genes. strains were compared to reference strains to differentiate m. ferrugineum from its relatives microsporum canis and microsporum audouinii. inter-species differences for t ... | 2012 | 21903828 |
| [fungi, pets and their owners]. | some parasitic or pathogen fungi for pet skin are possibly transmitted to humans in whom they are responsible for superficial mycozoonoses. cats, dogs and some small rodents are commonly involved. the lesions correspond to glabrous skin dermatophytoses and to microsporic tinea of the scalp. these disorders represent the vast majority of the prevalent mycozoonoses in wallonia. microsporum canis and trichophyton mentagrophytes are the two fungi that are commonly involved. | 2011 | 22216732 |
| antifungal activity of aegle marmelos (l.) correa (rutaceae) leaf extract on dermatophytes. | to evaluate the in vitro antifungal activity of aegle marmelos leaf extracts and fractions on the clinical isolates of dermatophytic fungi like trichophyton mentagrophytes, trichophyton rubrum, microsporum canis, microsporum gypseum and epidermophyton floccosum. | 2011 | 23569781 |
| [normative research on bacteriostasis and relieving itching external therapeutic function of kochiae fructus]. | to normalize bacteriostasis and relieving itching external therapeutic function of kochiae fructus. | 2012 | 23705363 |
| [tinea capitis etiology in ibn sina hospital in rabat (morocco)]. | tinea capitis (tc) is a contagious infection that affects mainly children and teenagers. a retrospective study was realized at the mycology-parasitology department of the ibn sina hospital in rabat, morocco. the study includes 125 cases of tc. the mean age is 12.73 ± 11.61 year. the isolation of tc is dominated by two species trichophyton violaceum 76 (60.8%) and microsporum canis 27 (21.6%). trichophyton verrucosum was isolated only in male and all of rural origin. in adults over 18 years, the ... | 2012 | 23518085 |
| design, spectral characterization and biological studies of transition metal(ii) complexes with triazole schiff bases. | a new series of three biologically active triazole derived schiff base ligands l(1)-l(3) have been synthesized in equimolar reaction of 3-amino-1h-1,2,4-triazole with pyrrol-2-carboxaldehyde, 4-bromo-thiophene-2-carboxaldehyde, and 5-iodo-2-hydroxy benzaldehyde. the prepared schiff bases were used for further complex formation reaction with different metal elements like co(ii), ni(ii), cu(ii) and zn(ii) as chlorides by using a molar ratio of ligand:metal as 2:1. the structure and bonding nature ... | 2012 | 23277183 |
| antimicrobial compounds from alpinia conchigera. | the rhizome of alpinia conchigerahas been used as a condiment in the northern states of peninsular malaysia and occasionally in folk medicine in the east coast to treat fungal infections. in some states of peninsular malaysia, the rhizomes are consumed as a post-partum medicine and the young shoots are prepared into a vegetable dish. this study aimed to investigate the chemical constituents of the pseudostems and rhizomes of malaysian alpinia conchigera and to evaluate the antimicrobial activity ... | 2012 | 23266278 |
| origins and evolution of the het-s prion-forming protein: searching for other amyloid-forming solenoids. | the het-s prion-forming domain from the filamentous fungus podospora anserina is gaining considerable interest since it yielded the first well-defined atomic structure of a functional amyloid fibril. this structure has been identified as a left-handed beta solenoid with a triangular hydrophobic core. to delineate the origins of the het-s prion-forming protein and to discover other amyloid-forming proteins, we searched for all homologs of the het-s protein in a database of protein domains and fun ... | 2011 | 22096554 |
| Enzymatic activity of Microsporum canis and Trichophyton mentagrophytes from breeding rabbits with and without skin lesions. | Microsporum canis and Trichophyton mentagrophytes are zoophilic dermatophytes which can cause skin infections in animals and humans. The clinical expression of this infection strongly varies depending on host, fungal species as well as enzyme production. No comparative studies are available on the enzymatic activities of M. canis and T. mentagrophytes isolated from breeding rabbits. Thus, the aim of this work was to assess the capability of M. canis and T. mentagrophytes isolated from rabbits bo ... | 2012 | 22175244 |
| in vitro antimicrobial activities of animal-used quinoxaline 1,4-di-n-oxides against mycobacteria, mycoplasma and fungi. | the quinoxaline 1,4-di-n-oxides (qdnos) were known as potent antibacterial agents. for the purpose of evaluating the bioactivity of existing animal-used qdnos drugs against representative pathogenic microorganism, the representative drugs of quinoxalines including cyadox, mequindox, quinocetone and their metabolites were submitted to the in vitro evaluation for antituberculosis, antimycoplasma, antifungal and antiviral activities. | 2016 | 27600955 |
| [tinea capitis in the university hospital of constantine (algeria)]. | although benign, tinea capitis are a public health problem and a frequent complaint in children. in algeria, these disorders have long been known; their high frequency was related to unfavorable social conditions of people both in cities than in rural areas. our aim is the study of tinea capitis diagnosed in the laboratory of parasitology and mycology of the university hospital of constantine through a retrospective review of 15 consecutive years from 1997 to 2011. currently the clinical and bio ... | 2012 | 23518171 |
| in vitro and in vivo antifungal activity of some essential oils against feline isolates of microsporum canis. | the treatment of dermatophytoses due to microsporum canis is cumbersome and relapses can occur. volatile essential oils (eos) obtained from plants would seem to represent suitable tools to contrast mycoses both in human and animals. the anti-m. canis activity of some eos chemically characterized was evaluated both in vitro and in vivo. eleven feline isolates of m. canis were tested by microdilution against eos extracted from thymus serpillum, origanum vulgare, rosmarinus officinalis, illicium ve ... | 2012 | 23518021 |
| identification of novel secreted proteases during extracellular proteolysis by dermatophytes at acidic ph. | the dermatophytes are a group of closely related fungi which are responsible for the great majority of superficial mycoses in humans and animals. among various potential virulence factors, their secreted proteolytic activity attracts a lot of attention. most dermatophyte-secreted proteases which have so far been isolated in vitro are neutral or alkaline enzymes. however, inspection of the recently decoded dermatophyte genomes revealed many other hypothetical secreted proteases, in particular aci ... | 2011 | 21919205 |
| antibacterial, antifungal and antioxidant activities of some medicinal plants. | the purpose of this study was to evaluate the antibacterial, antifungal and antioxidant activities of medicinal plants. the antibacterial activity of methanolic extracts of three medicinal plants (swertia chirata, terminalia bellerica and zanthoxylum armatum) were tested against gentamicin (standard drug) on eleven gram positive and seventeen gram negative bacteria by agar well method. it was revealed that seven-gram negative and six gram positive bacterial species were inhibited by these plant ... | 2014 | 26045377 |
| metal-based ethanolamine-derived compounds: a note on their synthesis, characterization and bioactivity. | metal-based ethanolamines, (l(1))-(l(4)) coordinated with co(ii), cu(ii), ni(ii) and zn(ii) metals in 1:2 (metal:ligand) molar ratio to produce new compounds have been reported. these compounds were screened for their bactericidal/fungicidal activity against a number of bacterial (escherichia coli, shigella flexneri, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus and bacillus subtilis) and fungal strains (trichophyton longifusus, candida albicans, aspergillus flavus, microsporum ... | 2016 | 27557038 |
| antiproliferative effect and characterization of a novel antifungal peptide derived from human chromogranin a. | cga-n46 is a novel antifungal peptide derived from the n-terminus of human chromogranin a, corresponding to the 31st to 76th amino acids. further research on its activities and characteristics may be helpful for the application of cga-n46 in medical or other situations. in the present study, the antifungal spectrum and physicochemical characteristics of cga-n46 were investigated using an antifungal assay, its antiproliferative effects on cancer and normal cells were assessed using mtt assay and ... | 2015 | 26668630 |
| dermatophyte and non dermatophyte fungi in riyadh city, saudi arabia. | dermatophytes are a scientific label for a group of three genera (microsporum, epidermophyton and trichophyton) of fungus that causes skin disease in animals and humans. conventional methods for identification of these fungi are rapid and simple but are not accurate comparing to molecular methods. | 2015 | 26288566 |
| discovery and characterization of a group of fungal polycyclic polyketide prenyltransferases. | the prenyltransferase (ptase) gene vrtc was proposed to be involved in viridicatumtoxin (1) biosynthesis in penicillium aethiopicum. targeted gene deletion and reconstitution of recombinant vrtc activity in vitro established that vrtc is a geranyl transferase that catalyzes a regiospecific friedel-crafts alkylation of the naphthacenedione carboxamide intermediate 2 at carbon 6 with geranyl diphosphate. vrtc can function in the absence of divalent ions and can utilize similar naphthacenedione sub ... | 2012 | 22590971 |
| antimicrobial metal-based thiophene derived compounds. | a novel series of thiophene derived schiff bases and their transition metal- [co(ii), cu(ii), zn(ii), ni(ii)] based compounds are reported. the schiff bases act as tridentate ligands toward metal ions via azomethine-n, deprotonated-n of ammine substituents and s-atom of thienyl moiety. the synthesized ligands along with their metal complexes were screened for their in vitro antibacterial activity against six bacterial pathogens (escherichia coli, shigella flexneri, pseudomonas aeruginosa, salmon ... | 2017 | 27766891 |
| report: antibacterial and antifungal activities of leaf extract of achyranthes aspera (amaranthaceae) from pakistan. | alcoholic extract and various fractions of achyranthes aspera leaves, traditionally used in pakistan for treatment of infectious diseases was screened for in vitro antibacterial and antifungal activity. the chloroform and butanol fractions were found to be the most active among the fractions, showing considerable antibacterial activity against shigella flexneri and escherichia coli. the highest activity was found in the ethylacetate fraction (17 mm zone of inhibition) against gram-negative (salm ... | 2015 | 26408900 |