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evaluation of antifungal activities of the essential oil and various extracts of nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains.plant extracts and plant-derived compounds are valuable sources as folk medicine for the treatment and prevention of a wide range of diseases including infectious diseases. in the present study, the antifungal activities of the essential oil and various extracts nigella sativa and its active principle, thymoquinone against trichophyton mentagrophytes, microsporum canis and microsporum gypseum as pathogenic dermatophyte strains have been evaluated. in addition, the cytotoxic effects of n. sativa ...201425442918
antifungal activity of pomegranate peel extract and isolated compound punicalagin against dermatophytes.dermatophyte species infect the epidermis and appendages, often with serious social and health-economic consequences. the hydroalcoholic extract of pomegranate fruit peel showed activity against the dermatophyte fungi trichophyton mentagrophytes, t. rubrum, microsporum canis and m. gypseum.201425260038
rapid detection for rabbit-derived dermatophytes using microsatellite-primed polymerase chain reaction.a method exhibiting high sensitivity, specificity and rapidity to detect pathogenic dermatophytes was developed using microsatellite-primed polymerase chain reaction (pcr) in combination with a clustering method. the dna fragments of trichophyton mentagrophyton, microsporum gypseum and microsporum canis were amplified by using the primer (gaca)4 to detect the dna polymorphism fingerprints. twenty-one clinical strains identified as t. mentagrophyton, m. gypseum or m. canis by morphological method ...201424296862
antifungal and cytotoxic activities of nannorrhops ritchiana roots extract.this atudy was designed to evaluate the antifungal and cytotoxic activities of the nannorrhops ritchiana (mazari palm) 80% methanol extract (nr-m) and its four crude extracts i.e., petroleum ether (nr-a), dichloromethane (nr-b), ethyl acetate (nr-c) and butanol (nr-d). the antifungal activity was determined by agar tube dilution method against nine fungal strains; aspergillus flavus, trichophyton longifusis, trichophyton mentagrophytes, aspergillus flavus and microsporum canis were susceptible t ...201425362807
metal-based carboxamide-derived compounds endowed with antibacterial and antifungal activity.a series of three bioactive thiourea (carboxamide) derivatives, n-(dipropylcarbamothioyl)-thiophene-2-carboxamide (l(1)), n-(dipropylcarbamothioyl)-5-methylthiophene-2-carboxamide (l(2)) and 5-bromo-n-(dipropylcarbamothioyl)furan-2-carboxamide (l(3)) and their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes (1)-(12) have been synthesized and characterized by their ir,(1)h-nmr spectroscopy, mass spectrometry and elemental analysis data. the crystal structure of one of the ligand, n-(dip ...201423914928
antibacterial, antifungal and antioxidant activities of some medicinal plants.the purpose of this study was to evaluate the antibacterial, antifungal and antioxidant activities of medicinal plants. the antibacterial activity of methanolic extracts of three medicinal plants (swertia chirata, terminalia bellerica and zanthoxylum armatum) were tested against gentamicin (standard drug) on eleven gram positive and seventeen gram negative bacteria by agar well method. it was revealed that seven-gram negative and six gram positive bacterial species were inhibited by these plant ...201426045377
acyclic sesquiterpenes released by candida albicans inhibit growth of dermatophytes.it is unresolved as to whether fungi that share a common skin habitat might in principal interact. in in vitro screening tests with candida albicans, trichophytum rubrum and other common dermatophytes, we found c. albicans releases volatile compounds that inhibit growth of the dermatophytes. by applying (enantioselective) gas chromatography combined with mass spectrometry we identified 8 compounds among which stereochemically pure (3r,6e)-2,3-dihydrofarnesol (r-dhf) and (2e,6e)-farnesol (f-ol) w ...201423902158
infectious diseases in large-scale cat hoarding investigations.animal hoarders accumulate animals in over-crowded conditions without adequate nutrition, sanitation, and veterinary care. as a result, animals rescued from hoarding frequently have a variety of medical conditions including respiratory infections, gastrointestinal disease, parasitism, malnutrition, and other evidence of neglect. the purpose of this study was to characterize the infectious diseases carried by clinically affected cats and to determine the prevalence of retroviral infections among ...201424934262
balticidins a-d, antifungal hassallidin-like lipopeptides from the baltic sea cyanobacterium anabaena cylindrica bio33.balticidins a-d (1-4), four new antifungal lipopeptides, were isolated from the laboratory-cultivated cyanobacterium anabaena cylindrica strain bio33 isolated from a water sample collected from the baltic sea, rügen island, germany. fractionation of the 50% aqueous meoh extract was performed by bioassay-guided silica gel column chromatography followed by spe and repeated reversed-phase hplc. the main fraction containing the compounds exhibited a strong and specific antifungal activity with inhib ...201424937366
pyrrolo[1,2-α][1,4]benzodiazepines show potent in vitro antifungal activity and significant in vivo efficacy in a microsporum canis dermatitis model in guinea pigs.pyrrolo[1,2-α][1,4]benzodiazepines (pbds) have been described as a novel class of antifungal compounds with activity against dermatophytes and aspergillus fumigatus. the initial structure-activity relationship showed that compounds with a chlorine substitution at position 7 have a higher activity compared with regioisomers or other substituents.201424535279
lawsonia inermis-mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel.lawsonia inermis mediated synthesis of silver nanoparticles (ag-nps) and its efficacy against candida albicans, microsporum canis, propioniabacterium acne and trichophyton mentagrophytes is reported. a two-step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. in addition, antimicrobial gel for m. canis and t. mentagrophytes was also formulated. ag-nps were synthesized by challenging the leaft ext ...201425082226
in vitro antifungal potentials of bioactive compound oleic acid, 3-(octadecyloxy) propyl ester isolated from lepidagathis cristata willd. (acanthaceae) inflorescence.to identify bioactive compound oleic acid, 3-(octadecyloxy) propyl ester from lepidagathis cristata willd. (l. cristata) and to assess antifungal potentials of the isolated compound.201425312119
antifungal, antileishmanial, and cytotoxicity activities of various extracts of berberis vulgaris (berberidaceae) and its active principle berberine.in this study, in vitro antidermatophytic activity against trichophyton mentagrophytes, trichophyton rubrum, microsporum canis, and microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (mic) using clsi broth macrodilution method (m38-a2). moreover, antileishmanial and cytotoxicity activity of b. vulgaris and berberine against promastigotes of leishmania major and leishmania tropica were evaluated by colorimetric mtt assay. the findings indicat ...201424977052
mycology - an update. part 1: dermatomycoses: causative agents, epidemiology and pathogenesis.dermatomycoses are caused most commonly by dermatophytes. the anthropophilic dermatophyte trichophyton rubrum is still the most frequent causative agent worldwide. keratinolytic enzymes, e.g. hydrolases and keratinases, are important virulence factors of t. rubrum. recently, the cysteine dioxygenase was found as new virulence factor. predisposing host factors play a similarly important role for the development of dermatophytosis of the skin and nails. chronic venous insufficiency, diabetes melli ...201424533779
[taxonomic study of clinic isolates of trichophyton in rosario, argentina].due to the pleomorphism and cultural variability displayed by species of the genus trichophyton, the identification methods based solely on morphological features are usually insufficient for their classification. the goal of the present work was to test a set of phenotypic methods in order to identify fungal isolates that belong to the aforementioned genus. these methods were based on a molecular taxonomic technique used as standard. clinical isolates (56) were used as samples along with 6 refe ...201424401778
characterization of a new sesquiterpene and antifungal activities of chemical constituents from dryopteris fragrans (l.) schott.one new sesquiterpene and six known compounds were isolated from dryopteris fragrans (l.) schot. they were identified as 3-o-β-d-glucopyranosylalbicanol- 11-o-β-d-glucopyranoside (1), dihydroconiferylalcohol (2), (e)-3-(4-hydroxyphenyl)acrylic acid (3), esculetin (4), 5,7-dihydroxy-2-hydroxymethylchromone (5), eriodictyol (6) and isoorientin (7) by uv, ms, 1d-nmr and 2d-nmr spectroscopy. the antifungal activities of the seven isolated compounds were screened. compounds 2, 3, 4 and 5 showed obvio ...201424451246
in vitro susceptibility of dermatomycoses agents to six antifungal drugs and evaluation by fractional inhibitory concentration index of combined effects of amorolfine and itraconazole in dermatophytes.to investigate the antifungal drug susceptibility of fungi responsible for dermatomycoses, minimum inhibition concentration (mic) tests were performed in 44 strains of dermatophytes, including trichophyton rubrum, trichophyton mentagrophytes, trichophyton verrucosum, trichophyton tonsurans, microsporum canis, microsporum gypseum and epidermophyton floccosum, with six antifungal drugs (amorolfine, terbinafine, butenafine, ketoconazole, itraconazole and bifonazole) by broth microdilution assay acc ...201424215461
therapeutic switching: from antidermatophytic essential oils to new leishmanicidal products.this study examined whether the antidermatophytic activity of essential oils (eos) can be used as an indicator for the discovery of active natural products against leishmania amazonensis. the aerial parts of seven plants were hydrodistilled. using broth microdilution techniques, the obtained eos were tested against three strains of dermatophytes (trichophyton mentagrophytes, microsporum gypseum and microsporum canis). to compare the eos antifungal and antiparasitic effects, the eos activities ag ...201525742270
use of restriction fragment length polymorphism to rapidly identify dermatophyte species related to dermatophytosis.dermatophytes are a group of keratinophilic fungi worldwide, which can infect the skin, hair and nails of humans and animals. this genus includes several species that present different features of dermatophytosis. although, laboratory diagnosis of dermatophytes is based on direct microscopy, biochemical tests and culture, these manners are expensive, time consuming and need skilled staff. therefore, molecular methods like pcr-rflp are the beneficial tools for identification, which are rapid and ...201526301058
solid lipid nanoparticles containing copaiba oil and allantoin: development and role of nanoencapsulation on the antifungal activity.the aim of this work was to develop solid lipid nanoparticles (sln) containing copaiba oil with and without allantoin (ncoa, nco, respectively) and to evaluate their antifungal activity. nanoparticle suspensions were prepared using a high homogenisation technique and characterised by dynamic light scattering, laser diffraction, nanoparticle tracking analysis, multiple light scattering analysis, high-pressure liquid chromatography, ph and rheology. the antifungal activities of the formulations we ...201525980176
myrtus communis l. as source of a bioactive and safe essential oil.in algeria, myrtus communis l. is distributed throughout the tell atlas and the coastal regions of algiers and constantine. the leaves are used in respiratory disorders, diarrhea and hemorrhoids. the aims of this work were to evaluate the antifungal and anti-inflammatory potential of well characterized essential oils (eo). since eo can be applied by inhalation, dermal application and oral administration, we used several mammalian cell lines to assess safe bioactive doses. the chemical compositio ...201525446467
dermatophytosis: a 16-year retrospective study in a metropolitan area in southern brazil.dermatophytoses are considered a public health problem. the objectives of this study were to determine the evolution of their prevalence in the metropolitan area of porto alegre, brazil, and to analyze the dermatophyte species distribution according to body site and demographic characteristics of the patients.201526322879
hemolytic activity of dermatophytes species isolated from clinical specimens.hemolytic activity was recently reported for several pathogenic fungal species, such as aspergillus, candida, trichophyton, penicillium and fusarium. based on a number of mechanistic and characterization studies, several fungal hemolysins have been proposed as virulence factors. hemolysins lyse red blood cells resulting in the release of iron, an important growth factor for microbes especially during infection. the requirement of iron in fungal growth is necessary for metabolic processes and as ...201525467819
antimicrobial and antioxidant activities and chemical characterization of essential oils of thymusvulgaris, rosmarinus officinalis, and origanum majorana from northeastern méxico.there have been no reports of antifungal activity and composition of extracts from thymus vulgaris, rosmarinus officinalis or origanum majorana from northeastern méxico. antifungal activity of these oils against trichophyton rubrum, trichophyton tonsurans, trichophyton mentagrophytes, microsporum gypseum, microsporum canis and epidermophyton floccosum was measured by diffusion assay. additionally, antibacterial and antioxidant activities were evaluated. antibacterial activity against staphylococ ...201525631514
in vitro antifungal activity of some traditional persian medicinal plants on pathogenic fungi.to investigate the antifungal activities of the extracts and sub-fractions of phlomis olivieri, verbascum speciosum, sambucus ebulus and erigeron hyrcanicus, four persian medicinal plants used in iranian folk medicine.201526129901
report: antibacterial and antifungal activities of leaf extract of achyranthes aspera (amaranthaceae) from pakistan.alcoholic extract and various fractions of achyranthes aspera leaves, traditionally used in pakistan for treatment of infectious diseases was screened for in vitro antibacterial and antifungal activity. the chloroform and butanol fractions were found to be the most active among the fractions, showing considerable antibacterial activity against shigella flexneri and escherichia coli. the highest activity was found in the ethylacetate fraction (17 mm zone of inhibition) against gram-negative (salm ...201526408900
molecular identification of fungal pathogens in nodular skin lesions of cats.in a retrospective study, we investigated 52 formalin-fixed, paraffin-embedded (ffpe) samples from cats with histologically confirmed cutaneous and subcutaneous mycoses to determine if the pathogens could be identified by molecular methods. aim of the study was to obtain a deep understanding of the spectrum of infectious agents, which, as we hypothesized, was not available by histopathology alone. detection of feline and fungal dna was achieved in 92.3% and 94.2% of the samples, respectively. mo ...201525550386
biologically active and thermally stable polymeric schiff base and its metal polychelates: their synthesis and spectral aspects.new metal polychelates of mn(ii), co(ii), ni(ii), cu(ii) and zn(ii) obtained by the interaction of metal acetates with polymeric schiff base containing formaldehyde and piperazine, have been investigated. structural and spectroscopic properties have been evaluated by elemental analysis, ft-ir and (1)h-nmr. geometry of the chelated polymers was confirmed by magnetic susceptibility measurements, uv-visible spectroscopy and electron spin resonance. the molecular weight of the polymer was determined ...201525955762
[2011 epidemiological survey of dermatomycoses in japan].an epidemiological survey of dermatomycoses and their causative fungus flora in japan for 2011 was conducted in accordance with methods and criteria of the past four surveys. the survey covered a total number of 36,052 outpatients who visited 12 dermatological clinics throughout japan. the results were as follows. 1)dermatophytosis was the most prevalent cutaneous fungal infection (2,980 cases) seen in these clinics, followed by candidiasis (378 cases) and then malassezia infections (152 cases). ...201526617109
inhibitory effect of silver nanoparticles mediated by atmospheric pressure air cold plasma jet against dermatophyte fungi.in an in vitro study with five clinical isolates of dermatophytes, the mic(50) and mic(100) values of silver nanoparticles (agnps) ranged from 5 to 16 and from 15 to 32 μg ml(- 1), respectively. the combined treatment of agnps with atmospheric pressure-air cold plasma (apacp) induced a drop in the mic(50) and mic100 values of agnps reaching 3-11 and 12-23 μg ml(- 1), respectively, according to the examined species. epidermophyton floccosum was the most sensitive fungus to agnps, while trichophyt ...201526296782
a multi-target approach toward the development of novel candidates for antidermatophytic activity: ultrastructural evidence on α-bisabolol-treated microsporum gypseum.multi-target strategies are directed toward targets that are unrelated (or distantly related) and can create opportunities to address different pathologies. the antidermatophytic activities of nine natural skin lighteners: α-bisabolol, kojic acid, β-arbutin, azelaic acid, hydroquinone, nicotinamide, glycine, glutathione and ascorbyl tetraisopalmitate, were evaluated, in comparison with the known antifungal drug fluconazole, on nine dermatophytes responsible for the most common dermatomycoses: mi ...201526132903
antidermatophytic activity of mikania micrantha kunth: an invasive weed.the incidence of dermatophytosis has risen dramatically in recent years. limited availability of side-effect free drugs has led to a search for new antidermatophytic agents.201526109783
anti-dermatophyte efficacy and environmental safety of some essential oils commercial and in vitro extracted pure and combined against four keratinophilic pathogenic fungi.establish new biocontrol practices with low persistence in the environment against dermatophyte causing mycosis.201525953414
dermatophyte and non dermatophyte fungi in riyadh city, saudi arabia.dermatophytes are a scientific label for a group of three genera (microsporum, epidermophyton and trichophyton) of fungus that causes skin disease in animals and humans. conventional methods for identification of these fungi are rapid and simple but are not accurate comparing to molecular methods.201526288566
design, synthesis and in vitro bactericidal/fungicidal screening of some vanadyl(iv)complexes with mono- and di-substituted ons donor triazoles.a new series of anti-bacterial and anti-fungal mono- and di-substituted triazoles (l(1))-(l(6)) have been synthesized and characterized on the basis of their physical, spectral and analytical data. the ligands (l(1))-(l(6)) on reaction with vanadyl(iv) sulphate led to the formation of vanadyl(iv) metal complexes (1)-(4). the structure of the complexes has been established on the basis of their physical, spectral and elemental analyses data. the synthesized ligands and their vanadyl(iv) complexes ...201525716124
antiproliferative effect and characterization of a novel antifungal peptide derived from human chromogranin a.cga-n46 is a novel antifungal peptide derived from the n-terminus of human chromogranin a, corresponding to the 31st to 76th amino acids. further research on its activities and characteristics may be helpful for the application of cga-n46 in medical or other situations. in the present study, the antifungal spectrum and physicochemical characteristics of cga-n46 were investigated using an antifungal assay, its antiproliferative effects on cancer and normal cells were assessed using mtt assay and ...201526668630
management of endemic microsporum canis dermatophytosis in an open admission shelter: a field study.endemic microsporum canis dermatophytosis was identified in a large, open admission, private, no-kill shelter that admitted >1200 cats per year. fungal culture (fc) screening revealed that 166/210 (79%) and 38/99 (38%) cats in the non-public and public area were culture positive, respectively. however, pending screening fc results, the 99 cats in the public area were treated with once-weekly lime sulfur rinses and monitored with once-weekly fc. cats in the non-public area were not treated. when ...201525074567
composition and antifungal activity of zhumeria majdae essential oil.essential oils extracted from different plants are extensively used in perfume, beverage, and food industries and are reported to exhibit antimicrobial activities against a variety of fungi. zhumeria majdae belonging to the lamiaceae family is a rare and endemic medicinal plant species in iran, with a strong and pleasant odor. the leaves of this plant have been used for many years as an antiseptic carminative agent for the treatment of stomachache (especially in infants) and dysmenorrhea.201528680999
[evaluation of the in vitro susceptibility pattern of clinical isolates of trichophyton mentagrophytes and trichophyton rubrum in santiago, chile].dermatophytes are a group of keratinophilic fungi able to produce dermatophytosis or tinea infections. in chile, trichophyton rubrum and trichophyton mentagrophytes are the ones most commonly isolated in adults, while microsporum canis is found among children. treatment of these infections is usually with topical or oral antifungals, such as griseofulvin or azole derivatives (clotrimazole, itraconazole, fluconazole), allylamines (terbinafine) or new drugs that are available.201524794211
assessment of immunogenicity and protective efficacy of microsporum canis secreted components coupled to monophosphoryl lipid-a adjuvant in a vaccine study using guinea pigs.microsporum canis is the most common dermatophyte in pets and is of zoonotic importance but currently there is no effective vaccine available to prevent dermatophytosis. the aim of this work was to assess the immunogenicity and protective efficacy of secreted components (sc) from m. canis adjuvanted with the monophosphoryl lipid-a (mpla), in a vaccine study using the guinea pig as an experimental model. animals were vaccinated with either the sc adjuvanted with the mpla, the mpla adjuvant alone ...201525532779
synthesis and fungistatic activity of aryl aldoxime derivatives.the antifungal activity of 13 arylaldoxime ester and ether derivatives was tested against 4 dermatophytes trichophyton mentagrophytes (tm), microsporum canis (mc); m. cookei, and m. gypseum.201525499806
newly described features resulting from high-magnification dermoscopy of tinea capitis.recent studies have reported "comma hairs" as a typical dermoscopic feature of tinea capitis observed at low magnification (×10). the aim of this study was to evaluate the dermoscopic aspects of tinea capitis at high magnification (×150) and its diagnostic role.201525471133
the anti-dermatophyte activity of allium hirtifolium boiss aqueous extract.in an attempt at demonstrating the efficacy of allium hirtifolium aqueous extract in control of skin fungal infections as traditional use, we evaluated the anti-dermatophyte activities of a. hirtifolium aqueous extract from bulbs and of ketoconazole against trichophyton rubrum, trichophyton mentagrophytes, microsporum canis, m. gypseum, trichophyton schoenleinii and trichophyton verrucosum var. album by food poisoning technique, disc diffusion and micro broth dilution assays. the anti-fungal act ...201525456419
phytochemical characterization, antimicrobial activity, and antioxidant potential of equisetum hyemale l. (equisetaceae) extracts.equisetum hyemale species is considered a medicinal plant used in the form of infusions to combat infectious or inflammation diseases and also diuretic effects, presenting several compounds related to these actions. in previous studies different species of equisetum showed several phenolic compounds. the objective of this study was, for the first time, based on phytochemistry analysis to evaluate the antioxidant and antimicrobial activity. the 70% ethanolic and methanolic extracts of e. hyemale ...201525587637
dermoscopic features in 12 cats with dermatophytosis and in 12 cats with self-induced alopecia due to other causes: an observational descriptive study.dermoscopy is a noninvasive technique allowing rapid magnified in vivo observation of the skin and structures that lie beneath the skin surface. various congenital and acquired hair shaft abnormalities may also be evaluated by dermoscopy. additionally, characteristic features of microsporum canis-induced tinea capitis and trichotillomania in humans have been reported.201525988302
tinea capitis caused by microsporum canis infection in a 75-year-old woman. 201525876151
digital gene expression analysis of microsporum canis exposed to berberine chloride.berberine, a natural isoquinoline alkaloid of many medicinal herbs, has an active function against a variety of microbial infections including microsporum canis (m. canis). however, the underlying mechanisms are poorly understood. to study the effect of berberine chloride on m. canis infection, a digital gene expression (dge) tag profiling was constructed and a transcriptome analysis of the m. canis cellular responses upon berberine treatment was performed. illumina/hisseq sequencing technique w ...201525874937
fingertip contamination after a brief touch of tinea capitis lesions caused by microsporum canis. 201524975367
[a recurrent case of adult favus successfully treated with terbinafine].favus or tinea capitis favosa, is a chronic inflammatory dermatophytosis of the scalp. the disease is particularly common in children aged 6 to 10 years, more often in boys, and it also occurs in adults. human-to-human transmission is therefore possible. anthropophilic trichophyton schöenleinii is responsible for over 95% of favus cases. in addition, there are rare cases of anthropophilic t.violaceum, zoophilic (t.verrucosum, t.quinckeanum, and microsporum canis) and geophilic m.gypseum species ...201526649420
tinea corporis, caused by microsporum canis - a case report from kosovo.tinea corporis (b35.6) caused by microsporum canis which is fungal species that causes numerous forms of disease. it is part of a group of fungi known as dermatophytes. though mostly well known for ringworm in pets, it is also known to infect humans. this fact makes this pathogen both anthrophilic and zoophilic in nature. microsporum canis is a communicable pathogen.201526622092
essential oil of algerian eucalyptus citriodora: chemical composition, antifungal activity.essential oil of eucalyptus citriodora is a natural product which has been attributed for various medicinal uses. in the present investigation, e. citriodora essential oil was used to evaluate its antifungal effect against medically important dermatophytes.201526597375
chemical composition and antifungal activity of essential oil from eucalyptus smithii against dermatophytes.in this study, we evaluated the chemical composition of a commercial sample of essential oil from eucalyptus smithii r.t. baker and its antifungal activity against microsporum canis atcc 32903, microsporum gypseum atcc 14683, trichophyton mentagrophytes atcc 9533, t. mentagrophytes atcc 11480, t. mentagrophytes atcc 11481, and trichophyton rubrum cct 5507.201526676500
in vitro and in vivo antidermatophytic activities of some iranian medicinal plants.in the last decades, the number of people suffering from dermatophytoses has seriously increased, which may be due to the development of resistant strains to a range of antifungal drugs. the present study was aimed to evaluate the antidermatophytic properties of eight extracts from the selected spices and herbs, which were ethno-medicinally used in iran against trichophyton mentagrophytes, trichophyton interdigitale, microsporum canis, and microsporum gypseum (10 strain of each). the in vitro an ...201526092105
in vitro evaluation of enzymatic and antifungal activities of soil-actinomycetes isolates and their molecular identification by pcr.human cutaneous infection caused by a homogeneous group of keratinophilic fungi called dermatophytes. these fungi are the most common infectious agents in humans that are free of any population and geographic area. microsporum canis is a cause of dermatophytosis (tinea) in recent years in iran and atypical strain has been isolated in iran. its cases occur sporadically due to m. canis transmission from puppies and cats to humans. since this pathogenic dermatophyte is eukaryotes, chemical treatmen ...201526060560
tinea faciei caused by trichophyton mentagrophytes in a 20-day-old neonate.although candidiasis in newborns is not uncommon, superficial dermatophyte infections of infants is quite rare. the causative agents of neonatal tinea reported in various case studies have been trichophyton rubrum, microsporum canis, microsporum gypseum, and trichophyton violaceum. to the best of our knowledge, no case report of neonatal tinea faciei caused by trichophyton mentagrophytes has been reported earlier.201526904450
epidemiological changes in tinea capitis over the sixty years of economic growth in china.tinea capitis is a fungal infection of the scalp occurring commonly in children. historical data indicate that clinical manifestations and the spectrum of etiologic agents vary greatly with geography, as well as socioeconomic affected populations.201526260745
griseofulvin and fluconazole reduce transmission of tinea capitis in schoolchildren.we evaluated the efficacy of griseofulvin and fluconazole in reducing the potential for person-to-person transmission of tinea capitis (tc) in children.201526215468
administration of oral itraconazole capsule with whole milk shows enhanced efficacy as supported by scanning electron microscopy in a child with tinea capitis due to microsporum canis.although diagnosis and treatment of tinea capitis in children are not difficult, treatment failures are still somewhat common. we report a case of pediatric tinea capitis cured using oral itraconazole administered with whole milk, after prior treatment failure when oral itraconazole was administered with water. this apparent enhanced efficacy in one individual was demonstrated using scanning electron microscopy.201526447934
[microsporum canis: current data on the prevalence of the zoophilic dermatophyte in central germany].microsporum (m.) canis, whose source of infection is mostly cats, is still considered as the most frequently occurring zoophilic dermatophyte in germany and europe. in distinct areas of germany, the zoophilic dermatophyte trichophyton (t.) anamorph of arthroderma (a.) benhamiae also presents a frequent and emerging causative agent of dermatophytoses.201526446665
antifungal activity of berberine hydrochloride and palmatine hydrochloride against microsporum canis -induced dermatitis in rabbits and underlying mechanism.phellodendron amurense, exhibits antifungal activity mainly by bioactive components including berberine hydrochloride and palmatine hydrochloride. this study was conducted to evaluate the antifungal effects of berberine hydrochloride, palmatine hydrochloride, and a mixture of both substances against microsporum canis in vivo and in vitro.201526054937
susceptibility of microsporum canis arthrospores to a mixture of chemically defined essential oils: a perspective for environmental decontamination.the zoophilic dermatophyte microsporum canis has cats as natural reservoir, but it is able to infect a wide range of hosts, including humans, where different clinical features of the so-called ringworm dermatophytosis have been described. human infections are increasingly been reported in mediterranean countries. a reliable control program against m. canis infection in cats should include an antifungal treatment of both the infected animals and their living environment. in this article, a herbal ...201525854840
updating the epidemiology of dermatophyte infections in palestine with special reference to concomitant dermatophytosis.to determine the epidemiology of dermatophytosis in palestinian patients, detect changes in the etiological agents during the last three decades, and to correlate between concomitant tinea pedis infections, and other cutaneous lesions.201525835157
kennel disinfectants for microsporum canis and trichophyton sp.the antifungal efficacy of commonly used kennel disinfectants for large surfaces was tested using naturally infective material from untreated animals (m. canis and trichophyton sp.) soaked and macerated but unfiltered leaving visible fluorescing hairs and/or scales in the test inoculum to create a robust challenge. disinfectants included sodium hypochlorite (1 : 32 and 1 : 100), enilconazole (1 : 100), accelerated hydrogen peroxide (1 : 16), potassium peroxymonosulfate (1% and 2%), and calcium h ...201525763290
incidence and etiological agents of genital dermatophytosis in males.to determine the incidence and etiological agents of dermatophytosis of male genitalia in sarajevo area, bosnia and herzegovina, during a 5-year period (2009-2013).201525669337
therapeutic switching: from antidermatophytic essential oils to new leishmanicidal products.this study examined whether the antidermatophytic activity of essential oils (eos) can be used as an indicator for the discovery of active natural products against leishmania amazonensis. the aerial parts of seven plants were hydrodistilled. using broth microdilution techniques, the obtained eos were tested against three strains of dermatophytes (trichophyton mentagrophytes, microsporum gypseum and microsporum canis). to compare the eos antifungal and antiparasitic effects, the eos activities ag ...201525714080
antifungal activity of cymbopogon nardus (l.) rendle (citronella) against microsporum canis from animals and home environment.dermatophytosis is a common zoonosis in urban centers. dogs and cats have played an important role as its disseminators. environmental decontamination is essential for the prevention of its propagation to humans and animals. however, sanitizers or disinfectants with antifungal activity, currently available, have high toxicity. the present study evaluated the in vitro effects of an extract of citronella (cymbopogon nardus) on 31 microsporum canis isolates from animals and home environments. susce ...201527049705
detection and characterization of zoonotic dermatophytes from dogs and cats in and around kolkata.the ringworms of pet dogs, cats, and stray animals (dogs, cats, and other animals) could be a potential source of zoonotic infections causing a serious public health problem in the busy city kolkata. the pet owners are more susceptible to get this infection from their pets, because of the close contact with them as dermatophytosis is very much prevalent in those pets. so, this study was aimed to check the prevalence of dermatophytosis in dogs, cats, and in pet owners.201527047202
in vitro activity of twenty commercially available, plant-derived essential oils against selected dermatophyte species.the in vitro activity of twenty chemically defined essential oils (eos) obtained from boswellia sacra, citrus bergamia, c. limon, c. medica, cinnamomum zeylanicum, eucalyptus globulus, foeniculum vulgare, helichrysum italicum, illicium verum, litsea cubeba, mentha spicata, myrtus communis, ocimum basilicum, origanum majorana, o. vulgare, pelargonium graveolens, rosmarinus officinalis, santalum album, satureja montana, and thymus serpyllum was assayed against clinical animal isolates of microspor ...201526434145
evaluation of two molecular techniques for rapid detection of the main dermatophytic agents of tinea capitis.tinea capitis is very common in western china, with the most widespread aetiological agent being trichophyton violaceum, while microsporum canis is prevalent in the remainder of china. conventional diagnostics and internal transcribed spacer (its) sequencing analyses have proven relatively limited due to the close phylogenetic relationship of anthropophilic dermatophytes. therefore, alternative molecular tools with sufficient specificity, reproducibility and sensitivity are necessary.201526342174
dermatoscopic findings as a complementary tool in the differential diagnosis of the etiological agent of tinea capitis.tinea capitis is a scalp infection caused by fungi. in brazil, the main causative agents are microsporum canis and the trichophyton tonsurans. etiological diagnosis is based on suggestive clinical findings and confirmation depends on the fungus growth in culture. however, it is not always possible to perform this test due to lack of availability. we reveal the dermoscopic findings that enable distinction between the main causative agents of tinea capitis, m. canis and t. tonsurans. the associati ...201526312662
a study on etiologic agents and clinical manifestations of dermatophytosis in yazd, iran.dermatophytosis is one of the most common infections of skin, hair, and nails, caused by a group of keratinophilic fungi known as dermatophytes. species identification of these fungi is of great significance from epidemiological and therapeutic points of view. the objective of the present study was to investigate dermatophytosis and its causative agents in patients, referring to the central mycology laboratory of yazd university of medical sciences, yazd, iran.201528681000
a survey of the etiological agents of scalp and nail dermatophytosis in yazd, iran in 2014-2015.tinea capitis and tinea unguium are regarded as global public health concerns. the purpose of the present study was to identify the etiological agents of tinea capitis and tinea unguium in patients, referring to the central laboratory of yazd university of medical sciences, yazd, iran.201528680997
antifungal and antioxidant activities of organic and aqueous extracts of annona squamosa linn. leaves.an increasing demand for natural additives has shifted the attention from synthetic to natural antioxidants and antifungal agents. this study was carried out to evaluate the antifungal and antioxidant activities of methanol, chloroform, and aqueous extracts of annona squamosa linn. leaves. the antifungal activities of all extracts of a. squamosa leaves against five different strains of fungi (alternaria alternata, candida albicans, fusarium solani, microsporum canis, and aspergillus niger) were ...201528911497
evaluation of antifungal effect of silver nanoparticles againstmicrosporum canis,trichophyton mentagrophytesandmicrosporum gypseum.dermatophytosis is the common cutaneous infections in humans and animals, which is caused by the keratinophylic fungus called dermatophytes. in recent years, drugs resistance in pathogenic fungi, including dermatophyte strains to the current antifungals have been increased.201528959308
the first case of onychomycosis in a koala (phascolarctos cinereus) due to atypical isolates of microsporum gypseum, a diagnostic challenge.superficial mycotic infections have been only poorly described in koalas and there are no reliable mycologically confirmed data regarding clinical isolation of dermatophytes in this animal. we report an 11-year-old female koala, kept in a zoo in tokyo, japan, and presenting with hyperkeratotic lesions and scaly plaques on forepaw claws and pads reminiscent of fungal infection.201628681020
proteolytic activity and cooperative hemolytic effect of dermatophytes with different species of bacteria.globally, dermatophytes are the most common filamentous group of fungi causing cutaneous mycoses. dermatophytes were shown to secrete a multitude of enzymes that play a role in their pathogenesis. there is limited data on co-hemolytic (camp-like) effect of different bacterial species on dermatophyte species. in this study, we sought to the evaluate exoenzyme activity and co-hemolytic effect of four bacteria on clinical dermatophytes isolated from patients in shiraz, iran.201628959790
the anti-dermatophyte activity of commiphora molmol.commiphora molmol engl (burseraceae) or myrrh has been traditionally used for the treatment of skin fungal infections.201626427766
epidemiology of superficial fungal infections in guangdong, southern china: a retrospective study from 2004 to 2014.superficial fungal infections are common worldwide; however, the distribution of pathogenic species varies among geographical areas and changes over time. this study aimed to determine the epidemiologic profile of superficial fungal infections during 2004-2014 in guangzhou, southern china. data regarding the superficial mycoses from outpatients and inpatients in our hospital were recorded and analyzed. from the 3367 patients that were enrolled in the study, 3385 samples were collected from skin, ...201626883512
determining the pathogenic potential of non-sporulating molds isolated from cutaneous specimens.although non-sporulating molds (nsm) are frequently isolated from patients and have been recognized as agents of pulmonary disease, their clinical significance in cutaneous specimens is relatively unknown. therefore, this study aimed to identify nsm and to determine the keratinolytic activity of isolates from cutaneous sites. nsm isolates from clinical specimens such as skin, nail, and body fluids were identified based on their ribosomal dna sequences. of 17 nsm isolates (7 ascomycota, 10 basidi ...201626847667
antifungal activity of cleome gynandra l. aerial parts for topical treatment of tinea capitis: an in vitro evaluation.cleome gynandra l. (capparaceae) is an edible weed used in uganda topically for its presumed antifungal activity against tinea capitis. the goal of this study was to determine if this plant possesses antifungal activity in vitro, since t. capitis is a pervasive infection among especially rural children.201627391957
evaluation of dermoscopy in the diagnosis of naturally occurring dermatophytosis in cats.a rapid, accurate screening test for dermatophytosis in cats is desirable in clinical and shelter medicine. in human dermatology, dermoscopy is used to identify dermatophyte-infected hairs by their characteristic comma hair appearance. similar "comma-like" hairs have been observed in infected cats.201627311364
in vitro evaluation of antifungal susceptibility and keratinase, elastase, lipase and dnase activities of different dermatophyte species isolated from clinical specimens in iran.the aims of this study were to evaluate the enzymatic activity of various dermatophyte species and their antifungal susceptibility profiles. a total of 60 dermatophyte isolates, including trichophyton mentagrophytes, trichophyton rubrum, microsporum canis and microsporum gypseum, were examined. fungal isolates were analysed for the production of keratinase, lipase, elastase and deoxyribonuclease (dnase). a broth microdilution method was performed on the basis of m38-a2 clinical and laboratory st ...201627291045
invasive microsporum canis causing rhinitis and stomatitis in a cat.microsporum canis is a pathogenic fungus that typically causes dermatophytosis in cats. this report describes a cat with a microsporum canis infection causing invasive fungal rhinitis that extended through the hard palate, resulting in adjacent stomatitis. treatment with itraconazole and terbinafine resolved the infection.201627061928
melokhanines a-j, bioactive monoterpenoid indole alkaloids with diverse skeletons from melodinus khasianus.the new melokhanines a-j (1-10) and 22 known (11-32) alkaloids were isolated from the twigs and leaves of melodinus khasianus. the new compounds and their absolute configurations were elucidated by extensive analysis of spectroscopic, x-ray diffraction, and computational data. melokhanine a (1), composed of a hydroxyindolinone linked to an octahydrofuro[2,3-b]pyridine moiety, is an unprecedented monoterpenoid indole alkaloid. melokhanines b-h (2-8) possess a new 6/5/5/6/6 pentacyclic indole alka ...201627584856
pcr and real-time pcr approaches to the identification of arthroderma otae species microsporum canis and microsporum audouinii/microsporum ferrugineum.the identification of species in the arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. microsporum canis is a zoophilic species, whereas microsporum audouinii and microsporum ferrugineum are anthropophilic species. in this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the a. otae complex methods: two pcr assays were designed based on differences ...201627306227
modeling dermatophytosis in reconstructed human epidermis: a new tool to study infection mechanisms and to test antifungal agents.dermatophytosis is a superficial fungal infection of keratinized structures that exhibits an increasing prevalence in humans and is thus requesting novel prophylactic strategies and therapies. however, precise mechanisms used by dermatophytes to adhere at the surface of the human epidermis and invade its stratum corneum are still incompletely identified, as well as the responses provided by the underlying living keratinocytes during the infection. we hereby report development of an in vitro mode ...201627760830
efficacy of topical therapy with newly developed terbinafine and econazole formulations in the treatment of dermatophytosis in cats.in the field of veterinary dermatology dermatophytosis is one of the most frequently occurring infectious diseases, therefore its treatment should be effective, convenient, safe and inexpensive. the aim of this study was to evaluate the efficacy of newly developed topical formulations in the treatment of cats with dermatophytosis. evaluation of clinical efficacy and safety of terbinafine and econazole formulations administered topically twice a day was performed in 40 cats. cats, suffering from ...201627760032
isolation and identification of microsporum canis from asian elephants (elephas maximus) in the chongqing zoo, china.skin diseases affect millions of people and animals worldwide, including asian elephants. this study sought to determine the pathogen of skin diseases that occurred in asian elephants in chongqing zoo, china. the isolated fungus was identified through its cultural characteristics, morphology, and polymerase chain reaction (pcr) amplification. the pcr amplification using common fungal primers (its1 and its4) determined that the pathogen was 99.7% homologous to microsporum canis. this is the first ...201627691957
tinea on a tattoo.in the last twenty years, the prevalence of individuals with tattoos in the general population has increased in europe (1) as well as in australia (2) and the united states of america (3). a series of complications such as acute inflammatory reactions, allergic contact dermatitis (4,5), photoinduced, lichenoid, and granulomatous reactions (6, 7), pseudolymphoma (8), pseudoepitheliomatous hyperplasia (9), skin infections (6), and skin cancers (10) may occur on tattoos. infectious complications on ...201627663926
detection of dermatophytes in healthy companion dogs and cats in eastern india.in recent times increasing occurrence of dermatophytosis, especially among the school children in eastern india was evidenced along with increased tendency of keeping companion animals such as dogs and cats. this study was undertaken to detect the occurrence of dermatophytes with antifungal susceptibility among the companion animals. a total of 1501 healthy companion animals comprising 1209 dogs and 292 cats belonged to individual owners in and around kolkata (west bengal, india) were examined f ...201627656224
[evolution of tinea capitis observed in mycology laboratory of institute pasteur of algeria from1995 to 2015].tinea capitis are common in algeria and are a frequent reason for consultation. this mycosis affects children and rarely adults. this is a retrospective study over a period of 20 years from 1995 to 2015 at the mycology laboratory of the pasteur institute of algeria.201627554867
diagnostic performance of molecular and conventional methods for identification of dermatophyte species from clinically infected arabian horses in egypt.rapid and accurate identification of dermatophytes is crucial for the effective control of disease outbreaks. current methods based on culture and microscopic characteristics may require weeks before positive identification is made.201627549079
[tinea in the genital area : a diagnostic and therapeutic challenge].pubogenital tinea or tinea genitalis represents a rare type of dermatophytosis which, however, is increasingly being diagnosed. the mons pubis is affected, but also the outer regions to the penis shaft and the labia together with the groins. pubogenital tinea is a more superficial erythrosquamous type, but strong inflammatory dermatomycoses of the genital area as tinea genitalis profunda ranging to kerion celsi are observed. a total of 30 patients (14-63 years of age, 11 men and 19 women) with p ...201627488308
prevalence of tinea capitis among school children in nok community of kaduna state, nigeria.in recent years, the prevalence of tinea capitis, an infection of the scalp by dermatophytes, has increased in children worldwide. this cross-sectional study was carried out to determine the prevalence and risk factor of tinea capitis among school children in nok community of kaduna state, nigeria. a total of 100 children were screened and 45% were diagnosed to have tinea capitis after fungal culture and microscopy. the prevalence of tinea capitis among girls was higher (51.4%) than that among b ...201627471603
antifungal effects of the biological agent pythium oligandrum observed in vitro.dermatophytosis, commonly known as ringworm, is a superficial fungal skin disease and zoonosis. pythium oligandrum is a micromycete with mycoparasitic properties that is used in agriculture to control fungal infections on plants. formulations containing p oligandrum were also developed for the treatment of dermatophytoses, but only several case studies have been published. in order to document the process in simplified conditions in vitro, we investigated the effectiveness of p oligandrum agains ...201627418287
[keratinophilic fungi in soils of parks of corrientes city, argentina].the soil is a natural reservoir of keratinophilic fungi, which are a small but important group of filamentous fungi, some of which typically develop on keratinized tissues of living animals. there are numerous species of saprophytic fungi with recognized keratinophilic abilities, and several studies have been undertaken in order to link their presence to possible human disease.201626112435
synthesis, biological evaluation and molecular docking of certain sulfones as potential nonazole antifungal agents.we reported herein the synthesis, antifungal activity, docking and in silico adme prediction studies of four novel series of sulfones 6a-f, 8a-c, 10a-f and 12a-c. all the newly synthesized sulfones were tested against four strains of candida (including fluconazole-resistant candida), two strains of aspergillus, two dermatophytic fungi (trichophytons mentagrophyte and microsporum canis) and syncephalastrum sp. with fluconazole as a reference drug. in general, compounds 8a and 10b showed selective ...201626805791
ailanthus altissima (miller) swingle fruit - new acyl β-sitosteryl glucoside and in vitro pharmacological evaluation.β-sitosterol-3-o-(6'-o-13"-octadecenoyl)-β-d-glucoside (1), a new acyl β-sitosteryl glucoside, along with three known compounds β-sitosterol-3-o-β-d-glucoside (2), β-sitosterol (3) and methyl gallate (4) have been isolated from the ethyl acetate soluble fraction of methanolic extract of ailanthus altissima fruits. their structures were elucidated through spectroscopic data including 2d nmr, esi-ms, methanolysis and oxidative cleavage of double bond. antibacterial, antifungal, cytotoxic, phytotox ...201626795253
an open-field study comparing an essential oil-based shampoo with miconazole/chlorhexidine for hair coat disinfection in cats with spontaneous microsporiasis.the goal of the present study was to compare the antifungal efficacy of an essential oil (eo) shampoo proven to be effective against microsporum canis with miconazole/chlorhexidine for topical hair coat disinfection in cats treated concurrently with oral itraconazole.201626783019
[tinea capitis in the military hospital avicenna (morocco): review of 8 years (2006-2013)].tinea capitis are due to fungal infection by dermatophytes. they are common in developing countries including morocco. the objective of this study intended to describe the epidemiology, clinical and mycological profile of tinea capitis in avicenna military hospital of marrakech.201626778722
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