Publications

identification of bacterial cells by chromosomal painting.	chromosomal painting is a technique for the microscopic localization of genetic material. it has been applied at the subcellular level to identify regions of eukaryotic chromosomes. here we describe the development of bacterial chromosomal painting (bcp), a related technology for the identification of bacterial cells. purified genomic dnas from six bacterial strains were labeled by nick translation with the fluorochrome fluor-x, cy3, or cy5. the average size of the labeled fragments was ca. 50 t ...	1997	9055426
evaluation and comparison of molecular techniques for epidemiological typing of salmonella enterica subsp. enterica serovar dublin.	a total of 28 unrelated isolates of the salmonella enterica subsp. enterica serovar dublin (s. dublin) collected during a 6-year period, as well as four samples of the s. dublin live vaccine strain bovisaloral and its prototype strain s. dublin 442/039, were investigated by different molecular typing methods for the following reasons: (i) to find the most discriminatory method for the epidemiological typing of isolates belonging to this salmonella serovar and (ii) to evaluate these methods for t ...	1996	8904430
molecular characterization of salmonella enterica subsp. enterica serovar choleraesuis field isolates and differentiation from homologous live vaccine strains suisaloral and sc-54.	four independent molecular methods were used to characterize the salmonella enterica subsp. enterica serovar choleraesuis live vaccine strains sc-54 and suisaloral and to differentiate them from s. choleraesuis field isolates. plasmid analysis revealed the presence of seven plasmid profiles. a virulence plasmid of 52-kbp was identified by hybridization with an spvb-spvc gene probe in each of the s. choleraesuis field isolates and in the suisaloral vaccine strain, but not in the sc-54 vaccine str ...	1996	8880500
phage types and ribotypes of salmonella enteritidis in southern italy.	differently from other european countries, southern italy was affected by a considerable increase in human infections due to salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) only after 1990. on the present investigation, two groups of s. enteritidis strains isolated during the low-incidence period 1980-1984 and the epidemic period 1990-1993, respectively, have been submitted to phage-typing and ribotyping in order to ascertain whether the epidemic increase was determined ...	1996	8861878
molecular typing of salmonella enterica subsp. enterica serovar enteritidis isolates.	a collection of 31 epidemiologically unrelated salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) isolates obtained during a 12-year period was characterised by different molecular typing methods. plasmid profile analysis, the detection of plasmid-encoded virulence genes and ribotyping allowed little or no further differentiation amongst these isolates. two different hybridisation patterns were observed by is200-typing of the s. enteritidis isolates. however, pulsed-field g ...	1996	8544212
[bacterial ascites caused by salmonella enterica subsp. enterica serotype indiana].		1993	8368710
identification of six open reading frames in the salmonella enterica subsp. enterica ser. typhi viab locus involved in vi antigen production.		1993	8248629
analysis of the antibiotic resistance detected in 2043 strains of salmonella enterica subsp. enterica isolated in stool cultures of spanish patients with acute diarrhoea (1986-1991).		1993	8125841
epidemic dissemination of salmonella enterica spp. enterica serovar bovismorbificans in southern italy in the years 1989-1991.	epidemic strains of salmonella enterica subsp. enterica serovar bovismorbificans isolated in southern italy during the years 1989-1991 were submitted to a molecular epidemiological study in comparison with isolates identified in the years 1980-1988 in the same geographic area. genomic dna fragments obtained by digestion with bgli or eco ri hybridized with escherichia coli rrna to produce three distinct, but highly related patterns. ribotype 1, which had never been identified before 1989, was fou ...	1994	7957796
[tetracycline-resistant plasmid of salmonella enteritidis isolated in kumamoto city].	we isolated salmonella choleraesuis subsp. choleraesuis serovar. enteritidis (s. enteritidis) from a mass of cases which broke out in september, 1991 in kumamoto city. the isolates were shown to hold plasmids on which a tetracycline (tc)-resistant gene was located. the plasmid, about 9 kb in size, was capable of expressing the gene in escherichia coli, unstable in s. enteritidis and escherichia coli. thus in order to investigate the creeping prevalence of the tc-resistant plasmid in kumamoto cit ...	1994	7829903
pulsed-field gel electrophoretic identification of salmonella enterica serovar typhimurium live vaccine strain zoosaloral h.	salmonella enterica subsp. enterica serovar typhimurium (salm. typhimurium) live vaccine strain zoosaloral h was characterized by pulsed-field gel electrophoresis (pfge). each of the two suitable restriction enzymes, xbai and spei, produced a unique restriction fragment pattern for this live vaccine strain which was not shared by field isolates of the same serovar. the characteristic fragment pattern proved to be stable during a 22 month observation period and was also not altered after animal p ...	1994	7765707
nucleotide sequence of iaga and iagb genes involved in invasion of hela cells by salmonella enterica subsp. enterica ser. typhi.		1995	7754225
use of ribotyping, is200 typing and plasmid analysis for the identification of salmonella enterica subsp. enterica serovar typhimurium vaccine strain zoosaloral h and its differentiation from wild type strains of the same serovar.	fifty salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) isolates obtained from one vaccinated and three non-vaccinated poultry flocks as well as the commercially available vaccine strain zoosaloral h and a s. typhimurium reference strain were characterized genotypically to differentiate between s. typhimurium live vaccine strain zoosaloral h and wild type strains of the same serovar. ribotyping revealed five different patterns one of which exclusively occurred in the vacci ...	1994	7727890
new extended-spectrum tem-type beta-lactamase from salmonella enterica subsp. enterica isolated in a nosocomial outbreak.	a new extended-spectrum beta-lactamase was detected in a lactose-positive salmonella enterica subsp. enterica strain that caused a nosocomial outbreak involving eight patients in a pediatric cardiology unit. this strain showed high levels of resistance to ceftazidime and aztreonam and relatively low levels of resistance to cefotaxime and ceftriaxone. resistance was associated with a conjugative plasmid of 59 kb, which encoded a new beta-lactamase with an isoelectric point of 5.9 that strongly hy ...	1995	7726515
[study of 2 food poisoning outbreaks in mora (toledo) with the same source of infection].	the occurrence of an outbreak needs an immediate investigation, because on it depends the necessary measures to control the spread of the epidemic, and it is also the way of knowing the causal factor. we present the results of the investigation of two food poisoning outbreaks (outbreak a and outbreak b) occurred in mora (toledo). both outbreaks were reported at the same time, the source of infection was the same and the total number of exposed people was 620.	1994	7618037
salt-induced filamentous growth of a salmonella strain isolated from blood.	a strain of salmonella choleraesuis subsp. choleraesuis serovar paratyphi-a isolated from the blood of a febrile patient grew into filaments on a nutrient agar containing various salts, such as nacl, kcl, mgcl2, nh4cl, (nh4)2so4, or (nh4)2hpo4, at concentrations of 50 to 400 mm. the filamentous cells were nonseptate and multinucleate, and they had colony-forming ability. this mutant strain, however, did not show filamentous growth in liquid media which contained the same salts. on nutrient agar ...	1986	3700602
virulence-associated plasmids of salmonella serotype typhimurium in experimental murine infection.	the growth pattern of salmonella enterica subsp. enterica serotype typhimurium (typhimurium) was studied in mice to examine the role of the 60-mdal virulence-associated plasmid in the pathogenesis of mouse typhoid. after repeated subcultures at 45 degrees c, isogenic variants harbouring the virulence-associated plasmid (strains c52, tm122 and tm332) or having lost this large plasmid (strains c53, tm123 and tm333) were obtained from three parental strains (strains c5, tm12 and tm33, respectively) ...	1986	3324943
[the aerobic intestinal flora of the microchiropteran bat chaerephon pumila in madagascar].	from stools of 88 malagasy insect eater bats chaerephon pumila, a large amount of bacterial strains were isolated. twenty different species were recognized, most of them belonged to enterobacteriaceae family. eight strains were identified as salmonella enterica subsp. enterica o48: -; 1.5, despite many atypical characters. strains of salmonella enteritidis, koserella trabulsii, kluyvera sp., odc negative serratia marcescens, atypical hafnia alvei... were also identified.	1988	3240566
occurrence of h-antigen z66 of r phase in cultures of salmonella serovar typhi originated from indonesia.	eighteen strains of salmonella choleraesuis subsp. choleraesuis serovar typhi (s. typhi) isolated from blood of patients in japan who had visited indonesia and returned before the onset of typhoid fever were found to possess the h-antigen z66 reported by guinée et al. (1981) but had the naturally occurring h-antigen d. they were not lysed by any of the phages of the vi phage typing system. after passage through semi-solid medium containing h-z66 antiserum, h-antigens of 11 of 18 cultures with h- ...	1988	3181315
experimental ovine salmonellosis (salmonella abortusovis): pathogenesis and vaccination.	salmonella enterica subsp. enterica ser. abortusovis, a sheep-adapted serotype, causes a contagious disease. abortion is the major symptom and the main source of contamination. research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. innate resistance partly controlled by the ity locus, increased resistance to reinfection and humoral and t-cell-mediated immunity were observations gained with a murine model. in ewes, abortion regularly occurs fo ...	1990	2101486
rrna probing of chromosomal dna of epidemic and sporadic isolates of salmonella enterica subsp. enterica serovar kottbus from northern and southern italy.	fifty-two strains of salmonella enterica subsp. enterica serovar kottbus, identified at the centres of enterobacteriaceae of northern and southern italy, were investigated by molecular genetic methods. thirteen isolates were recovered during two food-poisoning outbreaks that occurred in may 1987 in lombardy. the rdna gene restriction patterns, obtained by probing endonuclease cleaved chromosomal dna with photobiotin labeled escherichia coli rrna, revealed some heterogeneity among strains isolate ...	1990	2091942
abortion and serological reaction of ewes after conjunctival instillation of salmonella enterica subsp enterica ser abortusovis.	the aim of the experiment was to evaluate in pregnant ewes the clinical, bacteriological and serological effects of conjunctival instillation of s abortusovis. an individual dose of 1 x 10(10) viable s abortusovis were administered to 20 ewes 92 d after mating. among 18 pregnant ewes, 8 aborted 110 +/- 13 d after mating, 4 had stillborn lambs and 6 a clinically normal lambing (114 +/- 1.3 d). s abortusovis was isolated from placentas, swabs and foetuses of the 12 lambings. evolution of "h" agglu ...	1991	2042907
characterization of strains of salmonella enterica subsp. enterica serovar wien isolated in italy: an epidemiological evaluation.	in the years 1987-1988, 110 strains of wien serovar were isolated from a gastroenteritis outbreak in a neonatal unit in palermo (sicily). these strains showed different drug resistance patterns and plasmid profiles. analysis of endonuclease restriction fragments of chromosomal dna by hybridization with e. coli rrna has demonstrated that a single bacterial clone or its derivatives were responsible for the outbreak. furthermore, the study of 139 strains, isolated since 1970 from different geograph ...	1990	1982334
epidemiological evaluation by rrna-dna hybridization of strains of salmonella enterica subsp. enterica serovar abortusovis isolated in southern italy in the years 1981-1989.	salmonella enterica subsp. enterica serovar abortusovis is a major agent of abortion of the sheep and is firmly established, although at low prevalence, in sicily. this paper describes the application rdna gene restriction pattern fingerprinting to investigate relatedness among 7 serovar abortusovis strains isolated at the "istituto zooprofilattico sperimentale" of sicily and 29 isolates identified at the southern italy centre of enterobacteriaceae between 1981 and 1989. although abortusovis ser ...	1991	1727022
acid adaptation promotes survival of salmonella spp. in cheese.	salmonella typhimurium was adapted to acid by exposure to hydrochloric acid at ph 5.8 for one to two doublings. acid-adapted cells had increased resistance to inactivation by organic acids commonly present in cheese, including lactic, propionic, and acetic acids. recovery of cells during the treatment with organic acids was increased 1,000-fold by inclusion of 0.1% sodium pyruvate in the recovery medium. acid-adapted s. typhimurium cells survived better than nonadapted cells during a milk fermen ...	1992	1622286
plasmid profile analysis, phage typing, and antibiotic sensitivity of salmonella dublin from clinical isolates in the united states.	one hundred clinical isolates of salmonella choleraesuis subsp. choleraesuis serovar dublin (salmonella dublin) were examined for phage sensitivity, antibiotic resistance patterns, and plasmid content. computer analysis of the lysis patterns observed by using 27 typing phages divided the s. dublin isolates into 26 groups. one lytic pattern (designated pattern 16) contained 52% of the isolates examined whereas 16 isolates had unique patterns, and nine patterns had fewer than ten members. although ...	1992	1514237
[increase in the incidence of the typhimurium serotype of salmonella enterica (i) versus the classic enteritidis serotype].		1992	1489802
molecular typing of salmonella enterica subsp. enterica serovar wien by rrna gene restriction patterns.	analysis of digested dna from 40 salmonella enterica subsp. enterica serovar wien isolates revealed five different rrna gene restriction patterns for hindiii (h1 to h5) and five for psti (p1 to p5). the isolates had been selected on the basis of the year of isolation, the geographic origin and the antibiotic resistance pattern and were distinguished as follows: a) 13 pre-epidemic isolates from different french towns in 1958-69 and from senegal in 1968; b) 7 epidemic isolates from algiers in 1969 ...	1992	1488554
the role of 36 megadalton plasmid of salmonella enteritidis for the pathogenesis in mice.	the pathogenesis associated with the 36 megadalton (md) plasmid of salmonella choleraesuis subsp. choleraesuis serovar enteritidis (s. enteritidis) was assessed by using the plasmid-containing strain al1190, plasmid-cured strain al1192, and plasmid-reintroduced strain al1193. after oral inoculation of strain al1190 or al1192 to c57bl/6 mice, mesenteric lymph nodes, spleens, and livers were examined for the numbers of viable bacteria and for the histopathological changes. the numbers of bacteria ...	1992	1420563