Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| a whole-genome single nucleotide polymorphism-based approach to trace and identify outbreaks linked to a common salmonella enterica subsp. enterica serovar montevideo pulsed-field gel electrophoresis type. | in this study, we report a whole-genome single nucleotide polymorphism (snp)-based evolutionary approach to study the epidemiology of a multistate outbreak of salmonella enterica subsp. enterica serovar montevideo. this outbreak included 272 cases that occurred in 44 states between july 2009 and april 2010. a case-control study linked the consumption of salami made with contaminated black and red pepper to the outbreak. we sequenced, on the solid system, 47 isolates with xbai pfge pattern jixx01 ... | 2011 | 22003026 |
| tn1548-associated arma is co-located with qnrb2, aac(6')-ib-cr and blactx-m-3 on an incfii plasmid in a salmonella enterica subsp. enterica serovar paratyphi b strain isolated from chickens in china. | 2012 | 21965429 | |
| specific detection of campylobacter jejuni using the bacteriophage nctc 12673 receptor binding protein as a probe. | campylobacter jejuni is found in the intestines of poultry, cattle, swine, wild birds and pet animals and is the major cause of foodborne gastroenteritis in developed countries. we report the use of the receptor binding protein (rbp) of campylobacter bacteriophage nctc 12673 for the specific capture of campylobacter jejuni bacteria using rbp-derivatized capturing surfaces. the gp48 rbp was expressed as a glutathione s-transferase-gp48 (gst-gp48) fusion protein and immobilized onto surface plasmo ... | 2011 | 21955997 |
| pulsed-field gel electrophoresis supports the presence of host-adapted salmonella enterica subsp. enterica serovar typhimurium strains in the british garden bird population. | salmonellosis is a frequently diagnosed infectious disease of passerine birds in garden habitats within great britain with potential implications for human and domestic animal health. postmortem examinations were performed on 1,477 garden bird carcasses of circa 50 species from england and wales, 1999 to 2007 inclusive. salmonellosis was confirmed in 263 adult birds of 10 passerine species in this 11-year longitudinal study. a subset of 124 fully biotyped salmonella enterica subsp. enterica sero ... | 2011 | 21948838 |
| Phenotypic and molecular characterization of human salmonella enterica serovar 4,[5],12:i:- isolates in Slovakia. | Forty-three epidemiologically unrelated emerging Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strains isolated during the period 2009-2010 in Slovakia were characterized by phenotypic and genotypic methods. Thirty-one isolates (72.1%) expressed resistance to ampicillin, streptomycin, sulfizoxazole, and tetracycline [R-type ASSuT]. The majority of the strains belonged to both definitive phage types DT193 (30.2%) and U311 (27.9%). Other phage types identified were U302 (6.9%), DT18 (4. ... | 2011 | 21909783 |
| Prevalence of Salmonella enterica and the hygienic indicator Escherichia coli in raw meat at markets in Ouagadougou, Burkina Faso. | This study investigated the hygienic status and prevalence of Salmonella and Escherichia coli in retail meat sold at open markets in Ouagadougou, Burkina Faso. A total of 150 samples of beef meat (n = 45), beef intestine (n = 45), mutton (n = 30), and chicken (n = 30) were collected from four local markets for investigation. The prevalence of Salmonella enterica subsp. enterica was 9.3%, and six serotypes, all previously unreported in Burkina Faso, were identified: Derby, Tilene, Hato, Bredeney, ... | 2011 | 21902926 |
| comparison of two possible routes of pathogen contamination of spinach leaves in a hydroponic cultivation system. | the route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. three major bacterial pathogens, escherichia coli o157:h7, salmonella, and listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10⁶ and 10³ cfu/ml. in parallel, the pathogens were inoculated onto the growing leaf surface by pipetting, to give concentrations of 10⁶ and 10³ cfu per leaf ... | 2011 | 21902924 |
| Identification of environmental reservoirs of nontyphoidal salmonellosis: aptamer-assisted bioconcentration and subsequent detection of salmonella typhimurium by quantitative polymerase chain reaction. | In this study, identification of environmental reservoirs of Salmonella enterica subsp. enterica serovar Typhimurium (abbreviated as Salmonella Typhimurium) in sediments, water, and aquatic flora collected from the Ganges River (Ganges riverine material) was carried out by adopting a two-step strategy. Step 1 comprised a selective serovar-specific capture of Salmonella Typhimurium from potential reservoirs. Step 2 involved culture-free detection of selectively captured Salmonella Typhi ... | 2011 | 21875107 |
| Genome sequencing reveals diversification of virulence factor content and possible host adaptation in distinct subpopulations of Salmonella enterica. | Divergence of bacterial populations into distinct subpopulations is often the result of ecological isolation. While some studies have suggested the existence of Salmonella enterica subsp. enterica subclades, evidence for these subdivisions has been ambiguous. Here we used a comparative genomics approach to define the population structure of Salmonella enterica subsp. enterica, and identify clade-specific genes that may be the result of ecological specialization. | 2011 | 21859443 |
| methodologies for salmonella enterica subsp. enterica subtyping: gold standards and alternatives. | for more than 80 years, subtyping of salmonella enterica has been routinely performed by serotyping, a method in which surface antigens are identified based on agglutination reactions with specific antibodies. the serotyping scheme, which is continuously updated as new serovars are discovered, has generated over time a data set of the utmost significance, allowing long-term epidemiological surveillance of salmonella in the food chain and in public health control. conceptually, serotyping provide ... | 2011 | 21856826 |
| effects of two simulated oxytetracycline dosing regimens on horizontal transfer of antimicrobial resistance plasmids in an in vitro pharmacodynamic model. | to evaluate the impact of oxytetracycline exposure on horizontal transfer of an antimicrobial resistance plasmid. | 2011 | 21728847 |
| a multiplex real-time pcr assay targeting virulence and resistance genes in salmonella enterica serotype typhimurium. | abstract: | 2011 | 21707966 |
| multi-locus sequence typing of salmonella enterica subsp. enterica serovar enteritidis strains in japan between 1973 and 2004. | salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. this study determined s. enteritidis lineages before and after this pandemic event in japan using multilocus sequence typing (mlst). thirty s. enteritidis strains were collected in japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovi ... | 2011 | 21672260 |
| prevalence and counts of salmonella spp. in minimally processed vegetables in são paulo, brazil. | minimally processed vegetables (mpv) may be important vehicles of salmonella spp. and cause disease. this study aimed at detecting and enumerating salmonella spp. in mpv marketed in the city of são paulo, brazil. a total of 512 samples of mpv packages collected in retail stores were tested for salmonella spp. and total coliforms and escherichia coli as indication of the hygienic status. salmonella spp. was detected in four samples, two using the detection method and two using the counting method ... | 2011 | 21645825 |
| subtyping salmonella serovar enteritidis isolates from different sources using sequence typing based on virulence genes and crisprs. | salmonella enterica subsp. enterica serovar enteritidis is a major cause of foodborne salmonellosis in the united states. two major food vehicles for ser. enteritidis are contaminated eggs and chicken meat. improved subtyping methods are needed to accurately track specific strains of ser. enteritidis related to human salmonellosis throughout the chicken and egg food system. a sequence typing scheme based on virulence genes (fimh and ssel) and crisprs (clustered regularly interspaced short palind ... | 2011 | 21571881 |
| application of multiple locus variable number of tandem repeat analysis (mlva), phage typing and antimicrobial susceptibility testing to subtype salmonella enterica serovar typhimurium isolated from pig farms, pork slaughterhouses and meat producing plants in ireland. | salmonella enterica subsp. enterica serovar typhimurium is a common zoonotic pathogen encountered in irish pigs and the pork industry and its characterisation using highly discriminatory typing methods is necessary for epidemiological studies, outbreak investigation and control. multiple locus variable number of tandem repeat analysis (mlva), phage typing and antimicrobial susceptibility testing were applied to characterise 301 s. typhimurium isolates of porcine origin isolated from farms, slaug ... | 2011 | 21569956 |
| onset of diarrhea and pyrexia and time to detection of salmonella enterica subsp enterica in feces in experimental studies of cattle, horses, goats, and sheep after infection per os. | to determine time to first detection of salmonella organisms in feces of animals after experimental infection po and times to onset of diarrhea and pyrexia to evaluate a common method for identifying nosocomial infections on the basis of time of admission and onset of clinical signs (ie, the 3-day criterion). | 2011 | 21568780 |
| dry-resistance of salmonella enterica subsp. enterica serovar enteritidis is regulated by both sep22, a novel pathogenicity-related factor of salmonella, and nutrients. | environmental isolates of salmonella enterica serover enteritidis (s. enteritidis) clones were grown to the logarithmic phase, washed and re-suspended in saline or luria-bertani (lb) medium, and then 10-µl aliquots of the suspensions were dried overnight at room temperature. the dried bacteria were mixed with 1 ml of ice-cold pbs, suspended and examined for colony-forming activity. all of the pathogenic clones with high levels of sep22, identical to salmonella dps, maintained good viability if s ... | 2009 | 21566364 |
| inappropriate use of d-values for determining biocidal activity of various antimicrobials. | the objective of this study was to investigate the application of established d-value calculations to survival curves for various bacteria using the following antimicrobials: acidified sodium chlorite, triclosan, octanoic acid, and sodium hydroxide. d-values can be calculated in 3 ways, a linear regression, an endpoint calculation, or an average of multiple endpoint calculations. the assumption made in calculating a d-value is that the rate of kill follows 1st-order kinetics under specified trea ... | 2011 | 21535698 |
| rapid screening of epidemiologically important salmonella enterica subsp. enterica serovars using whole-cell maldi-tof mass spectrometry. | currently, 2,610 different salmonella serovars are described according to the white-kauffmann-le minor scheme. they are routinely differentiated by serotyping, which is based on the antigenic variability at lipopolysaccharide moieties (o antigens), flagellar proteins (h1 and h2 antigens), and capsular polysaccharides (vi antigens). the aim of this study was to evaluate the potential of maldi-tof mass spectrometry for rapid screening and identification of epidemiologically important salmonella en ... | 2011 | 21515723 |
| national outbreak of salmonella enteritidis phage type 14b in england, september to december 2009: case-control study. | we conducted an unmatched retrospective case–control study to investigate an upsurge of non-travel-related sporadic cases of infection with salmonella enterica subsp. enterica serotype enteritidis phage type 14b with antimicrobial resistance to nalidixic acid and partial resistance to ciprofloxacin (s. enteritidis pt 14b nxcp(l)) that was reported in england from 1 september to 31 december 2009. we analysed data from 63 cases and 108 controls to determine whether cases had the same sources of in ... | 2011 | 21507321 |
| equivalence of quality control strains of microorganisms used in the compendial microbiological tests: are national culture collection strains identical? | the pharmacopoeias list a number of microorganisms to be used in the compendial microbiological tests for confirming the growth-promoting, indicative, and inhibitory properties of the media and demonstrating the suitability of the test for a specific test article. major national culture collections are specified as the sources for these test strains based on their history of deposition and maintenance and use in the compendial tests. using these microorganisms, it has long been assumed that thes ... | 2010 | 21502014 |
| ultra-fast and sensitive detection of non-typhoidal salmonella using microwave-accelerated metal-enhanced fluorescence ("mamef"). | certain serovars of salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. a rapid, sensitive diagnostic test is needed to allow prompt initiation of therapy in individual patients and for measuring disease burden at the population level. an innovative and promising new rapid diagnostic technique is microwave-accelerated metal-enhanced fluorescence (mamef). we have adapted ... | 2011 | 21494634 |
| russian tortoises (agrionemys horsfieldi) as a potential reservoir for salmonella spp. | a total of 80 russian tortoises brought in poland were examined for presence of salmonella. salmonella was detected in 15 out of all the animals tested (18.75%). of the total of 56 strains, 30 (53.57%) belonged to salmonella enterica subsp. enterica (i) and 26 to salmonella enterica subsp. salamae (ii). the predominant serotype within subspecies i was s. newport, which is one of the most serotypes causing salmonellosis in humans and warm-blooded animals. in vitro determination of the susceptibil ... | 2011 | 21486674 |
| culling decisions of dairy farmers during a 3-year salmonella control study. | salmonella enterica subsp. enterica-serotypes lead to periodically increased morbidity and mortality in cattle herds. the bacteria can also lead to serious infections in humans. consequently, denmark has started a surveillance and control programme in 2002. the programme focuses on salmonella dublin which is the most prevalent and most persistent serotype in the danish cattle population. a field study in 10 dairy herds with persistent salmonella infections was carried out over three years to gai ... | 2011 | 21481960 |
| monoclonal antibodies to lipopolysaccharide antigens of salmonella enterica serotype typhimurium dt104. | salmonella enterica subsp. enterica serotype typhimurium is one of the major causative agents of human gastroenteritis. here we raised a panel of 45 monoclonal antibodies (mabs) against ser. typhimurium dt104 by immunizing mice with formalin-killed bacteria and demonstrated that all the mabs recognized the bacterial lipopolysaccharide (lps) antigen. these mabs were specific for group o:4 salmonella with very little or no cross-reactivity with other closely related bacteria and were able to bind ... | 2011 | 21466285 |
| antimicrobial resistance, virulence-associated genes, and pulsed-field gel electrophoresis profiles of salmonella enterica subsp. enterica serovar typhimurium isolated from piglets with diarrhea in korea. | salmonella enterica subsp. enterica serovar typhimurium was isolated from diarrheic piglets in 2 periods, 2000-2001 (n = 25) and 2005-2006 (n = 17). to compare the characteristics of the isolates collected during the 2 periods, all isolates were tested for antimicrobial resistance, the presence of virulence genes, and pulsed-field gel electrophoresis (pfge) patterns. all 42 isolates were resistant to at least 1 of the 20 antimicrobials tested, and 39 (93%) were resistant to 2 or more antimicrobi ... | 2011 | 21461195 |
| [investigation of potential risk factors for salmonella enterica subsp enterica serotype napoli: a nested case-control study in lombardia region]. | enter-net surveillance system has detected the re-emergence of salmonella enterica subsp enterica serotype napoli (s. napoli) since 2002. preliminary data show that food vehicle could not be the only one implicated in the transmission of this serotype. a nested case-control study has been conducted using data form a prospective cohort: for each salmonellosis notification in lombardia from may 2004 to december 2005 an ad hoc epidemiological investigation has been performed. cases have been define ... | 2010 | 21425643 |
| substructure within salmonella enterica subsp. enterica isolates from australian wildlife. | multilocus sequence typing of 56 salmonella enterica subsp. enterica strains isolated from australian wildlife hosts was performed. the results of population assignment algorithms revealed that the 56 strains could be subdivided into two distinct clades. strains belonging to the two clades were further distinguished phenotypically, genotypically, and with respect to host distribution. | 2011 | 21378038 |
| prevalence of salmonella spp. antibodies to toxoplasma gondii, and newcastle disease virus in feral pigeons (columba livia) in the city of jaboticabal, brazil. | the rock pigeon (columba livia) may serve as a reservoir for several pathogenic agents that can be transmitted to poultry, wildlife, domesticated pets, and/or humans via excreta, secretions, or dust from feathers. in addition, ingestion of infected pigeons by wild and domestic animals can also transmit these pathogenic agents. the health status of 126 free-living pigeons in an urban area was evaluated by microbiologic culture for salmonella and serologic testing for the presence of antibodies fo ... | 2010 | 21370639 |
| rapid identification of salmonella enterica serovars, typhimurium, choleraesuis, infantis, hadar, enteritidis, dublin and gallinarum, by multiplex pcr. | salmonella enterica subsp. enterica poses a threat to both human and animal health, with more than 2500 serovars having been reported to date. salmonella serovars are identified by slide and tube agglutination tests using o and h antigen-specific anti-sera, although this procedure is both labor intensive and time consuming. establishment of a method for rapid screening of the major salmonella serovars is therefore required. we have established multiplex polymerase chain reaction (m-pcr) assays f ... | 2011 | 21329737 |
| genome of a european fresh-vegetable food safety outbreak strain of salmonella enterica subsp. enterica serovar weltevreden. | the genome of salmonella enterica subsp. enterica serovar weltevreden strain 2007-60-3289-1 was sequenced. the genome sequence of this fresh-vegetable isolate from scandinavia will be useful for the elucidation of plant host factors in comparison to other serovars of s. enterica subsp. enterica. | 2011 | 21296964 |
| novel virulence gene and clustered regularly interspaced short palindromic repeat (crispr) multilocus sequence typing scheme for subtyping of the major serovars of salmonella enterica subsp. enterica. | salmonella enterica subsp. enterica is the leading cause of bacterial food-borne disease in the united states. molecular subtyping methods are powerful tools for tracking the farm-to-fork spread of food-borne pathogens during outbreaks. in order to develop a novel multilocus sequence typing (mlst) scheme for subtyping the major serovars of s. enterica subsp. enterica, the virulence genes ssel and fimh and clustered regularly interspaced short palindromic repeat (crispr) loci were sequenced from ... | 2011 | 21278266 |
| geographical and temporal dissemination of salmonellae isolated from domestic animal hosts in the culiacan valley, mexico. | the prevalence and diversity of salmonellae from domestic animal hosts were investigated in the culiacan valley, mexico. a total of 240 farm animal feces (cows, chicken, and sheep) were evaluated for salmonella spp. presence from july 2008 to june 2009. salmonella enterica subsp. enterica strains were isolated from 76 samples (31.7%), and 20 serotypes were identified being salmonella oranienburg (25%), salmonella give (14%), salmonella saintpaul (12%), and salmonella minnesota (11%) the most fre ... | 2011 | 21274525 |
| change in attachment of salmonella typhimurium, yersinia enterocolitica, and listeria monocytogenes to pork skin and muscle after hot water and lactic acid decontamination. | the attachment of salmonella enterica subsp. enterica serovar typhimurium, yersinia enterocolitica, and listeria monocytogenes to pig skin and muscle tissue decontaminated with 80 °c water or 55 °c, 1% lactic acid for 5 and 15s was investigated. attachment properties differed between skin and muscle surfaces. a significantly higher number of firmly attached bacteria was found on the decontaminated skin surface compared to the non-treated skin surface, both on hot water (p<0.0001) and on lactic a ... | 2010 | 21269717 |
| resistance to ampicillin, third-generation cephalosporins, ciprofloxacin, co-trimoxazole and azithromycin in clinical isolates of salmonella enterica subsp. enterica from germany: real problem or sporadic circumstance? | the objective of this study was to determine the in vitro activity of ampicillin, third-generation cephalosporins, ciprofloxacin, co-trimoxazole and azithromycin against salmonella enterica isolates. none of the isolates tested showed resistance to third-generation cephalosporins or azithromycin. the rates of resistance to ampicillin, co-trimoxazole and ciprofloxacin were 16.8%, 3.2% and 0.8%, respectively. moreover, 7.2% of the isolates showed reduced ciprofloxacin susceptibility, but none of t ... | 2011 | 21265586 |
| validation of a seminested pcr approach for rapid detection of salmonella enterica subsp. enterica serovar gallinarum. | salmonella enterica subsp. enterica serovar gallinarum (s. gallinarum) is the causative agent of fowl typhoid, one of the major causes of mortality and morbidity on poultry farms. even though it has been substantially eradicated in many developed countries, the disease still remains endemic in central and south america, in africa and in the mediterranean countries of europe. this leads to the routine screening of flocks, mainly by cultivation and serological techniques, which are expensive, as w ... | 2011 | 21256889 |
| tola mediates the differential detergent resistance pattern between the salmonella enterica subsp. enterica serovars typhi and typhimurium. | the tol-pal genes are essential for maintaining the outer membrane integrity and detergent resistance in various gram-negative bacteria, including salmonella. the role of tola has been well established for the bile resistance of salmonella enterica subsp. enterica serovar typhimurium. we compared the bile resistance pattern between the s. enterica serovars typhi and typhimurium and observed that typhi is more resistant to bile-mediated damage. a closer look revealed a significant difference in t ... | 2011 | 21252278 |
| efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees c. | an amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ... | 2011 | 21217035 |
| structures of the sep22 dodecamer, a dps-like protein from salmonella enterica subsp. enterica serovar enteritidis. | the crystal structure of sep22, a dna-binding protein from starved cells from salmonella enterica subsp. enterica serovar enteritidis, has been determined in two forms: the native state at 1.25 å resolution and an iron-soaked form at 1.30 å resolution. the sep22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. along the threefold axes of the 23 symmetry, hydrophilic asp channels that consist of asp ... | 2010 | 21206015 |
| a subtractively optimized dna microarray using non-sequenced genomic probes for the detection of food-borne pathogens. | in this study, we present the successful detection of food-borne pathogens using randomly selected non-sequenced genomic dna probes-based dna microarray chips. three food-borne pathogens, staphylococcus aureus, salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium), and bacillus cereus, were subjected for the preparation of the dna microarray probes. initially, about 50 dna probes selected randomly from non-sequenced genomic dna of each pathogen were prepared by using a set of ... | 2010 | 21116735 |
| chronic and acute infection of the gall bladder by salmonella typhi: understanding the carrier state. | despite major treatment and prevention efforts, millions of new typhoid infections occur worldwide each year. for a subset of infected individuals, salmonella enterica subsp. enterica serovar typhi colonizes the gall bladder and remains there long after symptoms subside, serving as a reservoir for the further spread of the disease. in this progress article, we explore recent advances in our understanding of the mechanisms by which salmonella spp.--predominantly s. typhi--colonize and persist in ... | 2010 | 21113180 |
| differential identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum based on polymorphic regions of glgc and spec genes. | salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum cause fowl typhoid and pullorum disease in avian species, respectively, and have been of considerable economic importance to the poultry industry in parts of the world. the definitive diagnosis of these diseases can be made only by isolation and identification of the causative agent. however, rapid identification of biovars gallinarum and pullorum is not easily feasible due to their common antigenic structure ... | 2010 | 21111918 |
| rapid multiple-locus variable-number tandem-repeats analysis of shigella spp. using multicolour capillary electrophoresis. | the multiple-locus variable-number tandem-repeats analysis (mlva) method for genotyping has proven to be a fast and reliable typing tool in several bacterial species. mlva is in our laboratory the routine typing method for salmonella enterica subsp. enterica serovar typhimurium, escherichia coli (two assays), listeria monocytogenes and yersinia enterocolitica. the gram-negative invasive enteropathogenic bacterium shigella is the most common cause of bacillary dysentery (shigellosis) worldwide, a ... | 2010 | 20951171 |
| the evolution and distribution of phage st160 within salmonella enterica serotype typhimurium. | summarysalmonellosis is an internationally important disease of mammals and birds. unique epidemics in new zealand in the recent past include two salmonella serovars: salmonella enterica subsp. enterica serovar typhimurium definitive type (dt) 160 (s. typhimurium dt160) and s. brandenburg. although not a major threat internationally, in new zealand s. typhimurium dt160 has been the most common serovar isolated from humans, and continues to cause significant losses in wildlife. we have identified ... | 2010 | 20950514 |
| high-throughput universal probe salmonella serotyping (upss) by nanopcr. | salmonella enterica subsp. enterica serovar identification is of great importance with respect to outbreak monitoring and case verification. therefore rapid, sensitive and cost efficient detection of salmonella spp. is indispensable within microbiology labs. to amalgamate single tube isolate identification with salmonella typing, we developed the high-throughput universal probe salmonella serotyping (upss) technique based on nano liter pcr. in comparison to the classical approach, where o- and h ... | 2010 | 20869995 |
| optical genetic mapping defines regions of chromosomal variation in serovars of s. enterica subsp. enterica of concern for human and animal health. | summaryinfections involving salmonella enterica subsp. enterica serovars have serious animal and human health implications; causing gastroenteritis in humans and clinical symptoms, such as diarrhoea and abortion, in livestock. in this study an optical genetic mapping technique was used to screen 20 field isolate strains from four serovars implicated in disease outbreaks. the technique was able to distinguish between the serovars and the available sequenced strains and group them in agreement wit ... | 2010 | 20860874 |
| salmonella enterica subsp. enterica in cattle egret (bubulcus ibis) chicks from central texas: prevalence, serotypes, pathogenicity, and epizootic potential. | cattle egrets have a worldwide distribution, feed in proximity to cattle and other domestic animals, and often nest in large colonies in urban woodlots. over a 3-yr period, nestlings from five cattle egret colonies from central texas, usa, were surveyed for salmonellosis. prevalence of infection ranged from 29% to 95%. seventeen salmonella enterica subsp. enterica serotypes were isolated, of which the 4,5,12:i-monophasic serotype predominated in cultures of both the digestive tract and pooled sp ... | 2010 | 20688631 |
| association between phylogeny, virulence potential and serovars of salmonella enterica. | salmonella enterica subsp. enterica is one of the leading causes of zoonotic food-borne disease worldwide. the consequence of these infections is a serious impact on economics of the society in the form of lost productivity and expenses for medical care. the objective of this study was to analyze the difference in genomic content between selected serovars, especially the content of pathogenicity genes and this was done with a dna microarray. furthermore, we investigated the phylogenetic relation ... | 2010 | 20656064 |
| the o28 antigen gene clusters of salmonella enterica subsp. enterica serovar dakar and serovar pomona are different. | a 10 kb o-antigen gene cluster was sequenced from a salmonella enterica subsp. enterica dakar o28 reference strain and from two s. pomona serogroup o28 isolates. the two s. pomona o antigen gene clusters showed only moderate identity with the s. dakar o28 gene cluster, suggesting that the o antigen oligosaccharides may contain one or more sugars conferring the o28 epitope but may otherwise be different. these novel findings are absolutely critical for the correct interpretation of molecular sero ... | 2010 | 20652070 |
| the prevalence of multidrug resistance is higher among bovine than human salmonella enterica serotype newport, typhimurium, and 4,5,12:i:- isolates in the united states but differs by serotype and geographic region. | salmonella represents an important zoonotic pathogen worldwide, but the transmission dynamics between humans and animals as well as within animal populations are incompletely understood. we characterized salmonella isolates from cattle and humans in two geographic regions of the united states, the pacific northwest and the northeast, using three common subtyping methods (pulsed-field gel electrophoresis [pfge], multilocus variable number of tandem repeat analysis [mlva], and multilocus sequence ... | 2010 | 20639364 |
| attachment and colonization by escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium, and staphylococcus aureus on stone fruit surfaces and survival through a simulated commercial export chain. | the ability of the foodborne pathogens escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium, and staphylococcus aureus to attach, colonize, and survive on stone fruit surfaces was investigated. fifty microliters of bacterial suspension was spot inoculated onto the sterile intact fructoplane of whole peaches and plums. minimum time required for initial adhesion and attachment was recorded for different surface contact times. surface colonizatio ... | 2010 | 20615337 |
| evaluation of antimicrobial activity of probiotic bacteria against salmonella enterica subsp. enterica serovar typhimurium 1344 in a common medium under different environmental conditions. | the importance of probiotics in human nutrition has been gaining recognition in recent years. these organisms have been shown to promote human health by enhancing immunological and digestive functions and fighting respiratory tract infections. we propose an improved in vitro model for the study of probiotic antimicrobial activity against enteropathogens, by attempting to re-create, in a common culture medium, environmental growth conditions comparable to those present in the small intestine. a p ... | 2010 | 20600855 |
| salmonella enterica serovar enteritidis tatb and tatc mutants are impaired in caco-2 cell invasion in vitro and show reduced systemic spread in chickens. | salmonella enterica subsp. enterica serovar enteritidis is a leading causative agent of gastroenteritis in humans. this pathogen also colonizes the intestinal tracts of poultry and can spread systemically in chickens. transfer to humans usually occurs through undercooked or improperly handled poultry meat or eggs. the bacterial twin-arginine transport (tat) pathway is responsible for the translocation of folded proteins across the cytoplasmic membrane. in order to study the role of the tat syste ... | 2010 | 20498258 |
| pork contaminated with salmonella enterica serovar 4,[5],12:i:-, an emerging health risk for humans. | salmonella enterica subsp. enterica serovar 4,[5],12:i:- is a monophasic variant of s. enterica serovar typhimurium (antigenic formula 4,[5],12:i:1,2). worldwide, especially in several european countries and the united states, it has been reported among the 10 most frequently isolated serovars in pigs and humans. in the study reported here, 148 strains of the monophasic serovar isolated from pigs, pork, and humans in 2006 and 2007 in germany were characterized by various phenotypic and genotypic ... | 2010 | 20472721 |
| serotypes and antimicrobial resistance profiles of salmonella isolates from pigs at slaughter in kenya. | salmonellosis is considered one of the most widespread food-borne zoonoses in industrialized as well as developing countries. the presence of salmonella in food animals at slaughter and the consequent cross-contamination of edible carcass tissues present a significant food safety hazard. | 2010 | 20440063 |
| investigation of an outbreak of salmonella enterica subsp. enterica serovar hadar food illness in the abruzzi region of italy. | a comparative study was made of 22 strains of salmonella hadar isolated from victims of an outbreak of food illness in the abruzzi region of italy in 2000 and 21 strains of the same serotype isolated from poultry meat and human stool samples in the abruzzi and molise regions between 2000 and 2001. the aim of the investigation was to provide an epidemiological interpretation of the food illness outbreak to establish the degree of similarity between the s. hadar strains isolated from victims of th ... | 2008 | 20405442 |
| proteins from latex of calotropis procera prevent septic shock due to lethal infection by salmonella enterica serovar typhimurium. | the latex of calotropis procera has been used in traditional medicine to treat different inflammatory diseases. the anti-inflammatory activity of latex proteins (lp) has been well documented using different inflammatory models. in this work the anti-inflammatory protein fraction was evaluated in a true inflammatory process by inducing a lethal experimental infection in the murine model caused by salmonella enterica subsp. enterica serovar typhimurium. | 2010 | 20371281 |
| a predominant multidrug-resistant salmonella enterica serovar saintpaul clonal line in german turkey and related food products. | recently, salmonella enterica subsp. enterica serovar saintpaul has increasingly been observed in several countries, including germany. however, the pathogenic potential and epidemiology of this serovar are not very well known. this study describes biological attributes of s. saintpaul isolates obtained from turkeys in germany based on characterization of their pheno- and genotypic properties. fifty-five s. saintpaul isolates from german turkeys and turkey-derived food products isolated from 200 ... | 2010 | 20363784 |
| dna microarray analysis of salmonella serotype typhimurium strains causing different symptoms of disease. | salmonella enterica subsp. enterica is one of the leading food-borne pathogens in the usa and european countries. outcome of human salmonella serotype typhimurium infections ranges from mild self-limiting diarrhoea to severe diarrhoea that requires hospitalization. increased knowledge of the mechanisms that are responsible for causing infection and especially the severity of infection is of high interest. | 2010 | 20356366 |
| effect of bovine lactoferrin in salmonella ser. typhimurium infection in mice. | lactoferrin (lf) has in vitro antimicrobial activity against gram-negative bacteria. salmonella enterica subsp. enterica serovar typhimurium causes systemic infection and acute diarrhea in humans, mainly in children younger than 2 years of age. the aim of the study was to determine the in vivo effect of bovine lf in salmonella ser. typhimurium infection in mice. 58 balb/c mice were employed. two hours before the infection with 300 microl of 10(7) cfu of salmonella ser. typhimurium, 29 mice recei ... | 2010 | 20306285 |
| inactivation of salmonella enteritidis strains by combination of high hydrostatic pressure and nisin. | the effects of high hydrostatic pressure (hhp) and nisin treatment alone and in combination on cellular components and viability of two salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) strains were evaluated by differential scanning calorimetry (dsc) and plate counting in order to evaluate the relative resistance and optimize the treatment conditions. s. enteritidis fda and osu 799 strains were subjected to hhp (0.1-550 mpa for 10 min at 25 degrees c) alone and in combina ... | 2010 | 20226554 |
| [study on the epidemiological characteristics and molecular typing of salmonella enterica subsp. enterica serovar senftenberg in shanghai]. | to study the molecular epidemiological characteristics of salmonella enterica subsp. enterica serovar senftenberg (salmonella senftenberg) in shanghai, from 2006 to 2007. | 2009 | 20193231 |
| pulsed-field gel electrophoresis diversity of human and bovine clinical salmonella isolates. | pulsed-field gel electrophoresis (pfge) characterization of 335 temporally and spatially matched clinical, bovine, and human salmonella enterica subsp. enterica isolates revealed 167 xbai pfge patterns. these isolates were previously classified into 51 serotypes and 73 sequence types, as determined by multilocus sequence typing. discriminatory power of pfge (simpson's index, d = 0.991) was considerably higher than that of multilocus sequence typing (d = 0.920) or serotyping (d = 0.913). although ... | 2010 | 20180633 |
| autonomous growth of isolated single listeria monocytogenes and salmonella enterica serovar typhimurium cells in the absence of growth factors and intercellular contact. | the aim of this study was to observe growth of isolated single bacterial cells in the absence of growth factors and intercellular contact. in order to exclude stochastic uncertainties induced by dilution series, a new micromanipulation method was developed to ensure explicit results under visual control. this was performed with particular care for production of single prokaryotic cells and subsequent investigation of their autonomous growth. over 450 single isolated listeria monocytogenes and sa ... | 2010 | 20173058 |
| evaluation of pet-related management factors and the risk of salmonella spp. carriage in pet dogs from volunteer households in ontario (2005-2006). | the purpose of this study was to determine pet-related management factors that may be associated with the presence of salmonella spp. in feces of pet dogs from volunteer households. from october 2005 until may 2006, 138 dogs from 84 households in ontario were recruited to participate in a cross-sectional study. five consecutive daily fecal samples were collected from each dog and enrichment culture for salmonella spp. was performed. a higher than expected number of the dogs (23.2%; 32/138) had a ... | 2011 | 20163574 |
| characterisation of pathogenic bacteria in a uasb-polishing pond system using molecular techniques. | molecular techniques have been commonly used to detect and quantify pathogenic bacteria in food, clinical and environmental samples, but in wastewater treatment plants few studies have been carried out. this work applied pcr with a specific set of primers to investigate pathogenic bacteria in a wastewater plant comprised of a uasb reactor followed by polishing ponds. in addition, in-situ hybridisation technique (fish) was used to estimate the abundance of escherichia coli in the system. accordin ... | 2010 | 20150719 |
| development and evaluation of a multiplex real-time polymerase chain reaction procedure to clinically type prevalent salmonella enterica serovars. | a multiplex real-time polymerase chain reaction procedure was developed to identify the most prevalent clinical isolates of salmonella enterica subsp. enterica. genes from the rfb, flic, fljb, and viab groups that encode the o, h, and vi antigens were used to design 15 primer pairs and taqman probes specific for the genes rfbj, wzx, flic, fljb, wcdb, the sdf-l sequence, and inva, which was used as an internal amplification control. the primers and probes were variously combined into six sets. th ... | 2010 | 20110454 |
| characterization of small cole1-like plasmids conferring kanamycin resistance in salmonella enterica subsp. enterica serovars typhimurium and newport. | multi-antibiotic resistant (mr) salmonella enterica serovars typhimurium and newport are an increasing concern in human and animal health. many strains are known to carry antibiotic resistance determinants on multiple plasmids, yet detailed information has been scarce. three plasmids conferring kanamycin (kan) resistance were isolated and nucleotide sequences were determined. two kan(r) plasmids from salmonella newport strains, psn11/00kan and psn02/01kan, were found to be identical and were 569 ... | 2010 | 20100512 |
| prevalence and risk factors for salmonella enterica subsp. enterica contamination in french breeding and fattening turkey flocks at the end of the rearing period. | an epidemiological study was conducted to estimate the prevalence of salmonella spp. contamination in french commercial breeding and fattening turkey flocks at the end of the rearing period, as part of a european union-wide baseline study. two hundred and five breeding turkey flocks and three hundred and two fattening turkey flocks were included in the study, between october 2006 and september 2007. the salmonella status of flocks was assessed by collecting five environmental faeces samples, ana ... | 2010 | 20044159 |
| virulotyping and antimicrobial resistance typing of salmonella enterica serovars relevant to human health in europe. | the combination of virulence gene and antimicrobial resistance gene typing using dna arrays is a recently developed genomics-based approach to bacterial molecular epidemiology. we have now applied this technology to 523 salmonella enterica subsp. enterica strains collected from various host sources and public health and veterinary institutes across nine european countries. the strain set included the five predominant salmonella serovars isolated in europe (enteritidis, typhimurium, infantis, vir ... | 2010 | 20039795 |
| tracing lineage by phenotypic and genotypic markers in salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and salmonella typhimurium isolated in state of são paulo, brazil. | fifty-three salmonella 1,4,[5],12:i:- and 45 salmonella typhimurium strains were characterised using phage typing, plasmid profiles and pulsed-field gel electrophoresis (pfge) for comparison. the majority of the strains were subdivided into definitive type (dt) 41 (22.6%) and dt 193 (18%) and the 60-mda plasmid was detected in 94.3% and 84.4% of strains, respectively. genetic diversity was observed among all strains and 90% presented a > 70% similarity through pfge analysis. these results sugges ... | 2009 | 20027476 |
| antimicrobial substances produced by coliform strains active against foodborne pathogens. | in the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in rio de janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. two strains (6.4%) exhibited inhibitory activity against the indicator strains, escherichia coli lmifrj and salmonella ente ... | 2010 | 19895262 |
| the use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment. | in the present study, chromogenic (red) bacteria were used to simulate actual target bacteria during set-up and optimization of an isolation process of bacteria, designed for food samples. isolation of bacteria from food in the context of molecular biological detection of food pathogens is a multistep process. development of such a separation method requires continuous monitoring of the location of the presumable targets in the sample tubes. therefore, red-coloured pigmented bacteria were used a ... | 2010 | 19874486 |
| infected abdominal aortic aneurysm caused by nontyphoid salmonella in an immunocompromised patient with rheumatoid arthritis. | nontyphoid salmonella strains are important pathogens commonly found worldwide, typically causing gastrointestinal illness. here, we report a case of a 66-yearold man with an abdominal aortic infected (or so-called mycotic) aneurysm caused by salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis). he had multiple risk factors for atherosclerosis: age over 60, a long history of smoking, an 8-year history of diabetes mellitus, and a 10-year history of rheumatoid arthritis treated ... | 2009 | 19856069 |
| seroprevalence of antibodies against salmonella enterica subsp. enterica serovar gallinarum-pullorum in wild doves (zenaida asiatica and zenaida macroura) from the northeast of mexico. | a cross-sectional survey was conducted in order to determine the seroprevalence and to identify some factors associated with the presence of antibodies against salmonella enterica subsp. enterica serovar gallinarum-pullorum in white-winged (zenaida asiatica) and mourning doves (zenaida macroura) from hunting areas of northeast mexico. from september to october 2006, 201 serum samples were analyzed with the seroagglutination test. the overall seroprevalence of s. enterica subsp. enterica serovar ... | 2010 | 19800701 |
| real-time fret pcr assay for salmonella enterica serotype detection in food. | salmonella enterica subsp. enterica serotypes are leading etiological agents of food-borne gastroenteritis. traditional identification is laborious and time intensive. faster molecular methods may allow early identification in contaminated food products. we developed a real-time, fluorescence resonance energy transfer hybridization probe polymerase chain reaction (pcr) assay for s. enterica serotypes on the basis of the exclusive presence of the apee gene in salmonella typhimurium. assay sensiti ... | 2009 | 19780376 |
| spi-7: salmonella's vi-encoding pathogenicity island. | salmonella pathogenicity island-7 (spi-7) is a large, mosaic, genetic island, found in several serovars of salmonella enterica subsp. enterica associated with systemic disease. as well as encoding genes which may aid its own transmission, it carries genes for potential virulence factors such as vi antigen, sope effector and type ivb pili. the stability of spi-7 is of interest with respect to typhoid fever and related vaccines. | 2008 | 19741287 |
| o-antigen delays lipopolysaccharide recognition and impairs antibacterial host defense in murine intestinal epithelial cells. | although toll-like receptor (tlr) 4 signals from the cell surface of myeloid cells, it is restricted to an intracellular compartment and requires ligand internalization in intestinal epithelial cells (iecs). yet, the functional consequence of cell-type specific receptor localization and uptake-dependent lipopolysaccharide (lps) recognition is unknown. here, we demonstrate a strikingly delayed activation of iecs but not macrophages by wildtype salmonella enterica subsp. enterica sv. (s.) typhimur ... | 2009 | 19730692 |
| gamma radiation used as hygienization technique for foods does not induce viable but non-culturable state (vbnc) in salmonella enterica subsp. enterica serovar typhimurium. | gamma radiation has been widely used for hygienization of food products. whether gamma radiation stress induces vbnc state in salmonella is of great concern. therefore, the study was carried out to determine whether gamma radiation exposure induces vbnc state in salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium). the parameters tested were culturability on agar medium, transcriptional activity by rt-pcr, cytoplasmic membrane integrity, and direct viable count using live/dea ... | 2009 | 19641961 |
| virulotyping of salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in malaysia using multiplex-pcr. | the increased occurrence of salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. this study is aimed to detect the prevalence of twenty different virulence factors among salmonella enterica strains isolated from poultry and local indigenous vegetables in malaysia via an optimized, rapid and specific multiplex pcr assay. the assay encompasses a total of 19 salmonella pathogenicity islands genes and a quorum sensing gene (sdia) in three multiplex ... | 2009 | 19565351 |
| reliable means of diagnosis and serovar determination of blood-borne salmonella strains: quick pcr amplification of unique genomic loci by novel primer sets. | typhoid fever is becoming an ever increasing threat in the developing countries. we have improved considerably upon the existing pcr-based diagnosis method by designing primers against a region that is unique to salmonella enterica subsp. enterica serovar typhi and salmonella enterica subsp. enterica serovar paratyphi a, corresponding to the sty0312 gene in s. typhi and its homolog spa2476 in s. paratyphi a. an additional set of primers amplify another region in s. typhi ct18 and s. typhi ty2 co ... | 2009 | 19535522 |
| salmonella expressing a t-cell epitope from sendai virus are able to induce anti-infection immunity. | bacterial fimbriae can accept foreign peptides and display them on the cell surface. a highly efficient gene replacement method was used to generate peptide vaccines based on salmonella enterica subsp. enterica serovar typhimurium lt2. dna encoding an epitope from sendai virus, sv9 (sendai virus nucleoprotein peptide 324-332, fapgnypal), which is known to induce cytotoxic t lymphocytes, was incorporated into the gene encoding agfa (the major subunit protein of thin aggregative fimbriae of salmon ... | 2009 | 19528162 |
| turtle-associated salmonella septicaemia and meningitis in a 2-month-old baby. | a severe case of reptile-associated salmonellosis which caused septicaemia and meningitis in a 2-month-old baby is reported. the infrequent serotype salmonella enterica subsp.(i) enterica serotype abony (4,5 : b : enx) was detected in the human sample as well as in the pet turtle's faeces. the importance of regulation and public awareness is highlighted. | 2009 | 19528160 |
| dna microarray for molecular epidemiology of salmonella. | salmonellosis is a common infection estimated to affect 3 billion people and to cause 200,000 deaths every year. infections can appear as enteric fever, gastroenteritis, bacteremia, or extraintestinal focal infection. the course of the disease depends on a variety of factors, including infective dose, immune status of the host, and the genetic background of both the host and the pathogen. it has been recognized that certain salmonella types play a major role in the epidemiology of salmonella. he ... | 2009 | 19521880 |
| [characterisation of a phenotypic monophasic variant belonging to salmonella enterica subsp. enterica serovar typhimurium from wild birds and its possible transmission to cats and humans]. | in the last two years the national salmonella reference laboratory (nrl-salm) received an accumulating number of salmonellae with sero-formula 4,12:-:1,2 isolated from perished wild birds, particularly siskins. within these strains flagellar antigen of the first phase was phenotypically not detectable. by pcr a fragment could be amplified coding specifically for the h:i-flagellar antigen. consequently, this is a phenotypically monophasic variant of salmonella enterica subsp. enterica serovar typ ... | 2009 | 19517930 |
| analysis of antimicrobial resistance and plasmid profiles in salmonella serovars associated with tropical seafood of india. | a total of 256 salmonella strains consisting of 29 salmonella serovars isolated from seafood of cochin (india) were analyzed for resistance to antimicrobials commonly used in human and veterinary medicines as therapeutic agents. the 10 most predominant salmonella serovars in seafood were also characterized for presence of plasmids using the alkaline lysis method. antimicrobial susceptibility studies highlighted a comparatively high resistance in salmonella isolates to sulfamethizol and carbenici ... | 2009 | 19422307 |
| effects of static magnetic field on cell growth, viability, and differential gene expression in salmonella. | in the present study, we investigated the effect of exposure to a static magnetic field (smf) on cell growth, viability, and gene expression of salmonella enterica subsp. enterica serovar hadar. our results indicated that smf exposure (200 mt, 13 hours) failed to alter cellular growth but induced a decrease of colony-forming units (cfu) between 3 and 6 hours followed by an increase from 6 to 9 hours. the analysis of the differential expression of rpoa, dnak, katn, and 16s rrna genes under smf ex ... | 2009 | 19422305 |
| search for salmonella spp. in ostrich productive chain of brazilian southeast region. | we analyzed ostriches from an equipped farm located in the brazilian southeast region for the presence of salmonella spp. this bacterium was investigated in 80 samples of ostrich droppings, 90 eggs, 30 samples of feed and 30 samples of droppings from rodents. additionally, at slaughter-house this bacterium was investigated in droppings, caecal content, spleen, liver and carcasses from 90 slaughtered ostriches from the studied farm. also, blood serum of those animals were harvested and submitted ... | 2009 | 19390983 |
| salmonella enterica serovar enteritidis pathogenicity island 1 is not essential for but facilitates rapid systemic spread in chickens. | salmonella enterica subsp. enterica serovar enteritidis is a leading cause of human food-borne illness that is mainly associated with the consumption of contaminated poultry meat and eggs. to cause infection, s. enteritidis is known to use two type iii secretion systems, which are encoded on two salmonella pathogenicity islands, spi-1 and spi-2, the first of which is thought to play a major role in invasion and bacterial uptake. in order to study the role of spi-1 in the colonization of chicken, ... | 2009 | 19364835 |
| characterization of pathogenic and resistant genome repertoire reveals two clonal lines in salmonella enterica subsp. enterica serovar paratyphi b (+)-tartrate positive. | a total of 36 contemporary human, animal, and environmental (+)-tartrate-fermenting (dt+) salmonella enterica serovar paratyphi b isolates, formerly called salmonella serovar java, and five related monophasic s. enterica serovar 4,5,12:b:- isolates from belgium, germany, the netherlands, and the united kingdom were investigated for clonality and antimicrobial resistance profiles, as well as their virulence and resistance gene repertoire. two major clonal lines, which could be phenotypically diff ... | 2009 | 19292689 |
| emergence, distribution, and molecular and phenotypic characteristics of salmonella enterica serotype 4,5,12:i:-. | salmonella spp. represent one of the most common causes of bacterial foodborne illnesses around the world. the species salmonella enterica contains more than 2500 serotypes, and emergence of new human pathogenic salmonella strains and serotypes represents a major public health issue. salmonella enterica subsp. enterica serotype 4,5,12:i:- represents a monophasic variant of salmonella typhimurium, which has rarely been identified before the mid-1990 s. the prevalence of this serotype among human ... | 2009 | 19292687 |
| risk factors for salmonella enterica subsp. enterica contamination in 519 french laying hen flocks at the end of the laying period. | a cross-sectional study was carried out to identify risk factors for salmonella spp. contamination in french laying hen flocks at the end of the laying period. five hundred and nineteen flocks were studied between october 2004 and september 2005. the salmonella status of the flocks was assessed from 5 faeces samples (pooled faeces samples from cage flocks and foot swabs from flocks kept on the floor, i.e. in a barn, outdoors and on organic farms) and 2 dust samples analysed using a classical bac ... | 2009 | 19237216 |
| distribution and genotypic characterization of salmonella serovars isolated from tropical seafood of cochin, india. | to determine the distribution of salmonella serovars in seafood and to examine the intraserovar genetic variations in salmonella enterica subsp. enterica serovar rissen and salmonella weltevreden by polymerase chain reaction (pcr)-ribotyping and enterobacterial repetitive intergenic consensus (eric)-pcr methods. | 2009 | 19200318 |
| importance of subunit vaccine antigen of major fli c antigenic site of salmonella enteritidis ii: a challenge trial. | salmonella enterica subsp. enterica serovar enteritidis (se) infection in chickens shows a mild pathogenicity except for young ages, compared with other animals, and laying hens sometimes produce se-contaminated eggs leading to public health concerns. to reduce the problem, se bacterin in poultry farms has been applied. we previously demonstrated that a subunit antigen, g.m. part polypeptide in se-fli c (sep 9), could be a candidate subunit antigen of se vaccine which may show less side effects ... | 2009 | 19186198 |
| recovery of salmonella from bermudagrass exposed to simulated wastewater. | most confined swine (sus scrofa) feeding operations in the southeastern united states hold manure in lagoons and apply effluent on bermudagrass [cynodon dactylon (l.) pers.] as fertilizer. salmonella enterica subsp. enterica (ex kauffman and edwards) le minor and popoff, has been reported in mississippi lagoons, but levels and potential for contamination of bermudagrass were unknown. a laboratory method was developed to examine salmonella contamination of bermudagrass and levels of salmonella we ... | 2009 | 19141824 |
| poultry-associated salmonella enterica subsp. enterica serovar 4,12:d:- reveals high clonality and a distinct pathogenicity gene repertoire. | a european baseline survey during the years 2005 and 2006 has revealed that the monophasic salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in german broiler flocks. in denmark and the united kingdom, its serovar prevalences were 15.15% and 2.8%, respectively. although poultry is a major source of human salmonellosis, serovar 4,12:d:- is rarely isolated in humans (approximately 0.09% per year). molecular typing studies usi ... | 2009 | 19114530 |
| development of liposome immunoassay for salmonella spp. using immunomagnetic separation and immunoliposome. | the ability to detect salmonella spp. is essential in the prevention of foodborne illness. this study examined a salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (ims/il) encapsulating sulforhodamine b (srb), a fluorescent dye. a quantitative assay was conducted by measuring the fluorescence intensity of srb that was produced from an immunomagnetic bead-salmonella spp.-immunoliposome complex. the results indicated detection limits of 2.7 ... | 2008 | 18955821 |
| rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry . | variations in the mass spectral profiles of multiple housekeeping proteins of 126 strains representing salmonella enterica subsp. enterica (subspecies i), s. enterica subsp. salamae (subspecies ii), s. enterica subsp. arizonae (subspecies iiia), s. enterica subsp. diarizonae (subspecies iiib), s. enterica subsp. houtenae (subspecies iv), and s. enterica subsp. indica (subspecies vi), and salmonella bongori were analyzed to obtain a phylogenetic classification of salmonellae based on whole-cell m ... | 2008 | 18952875 |
| comparative analysis of protein profiles of wild virulent (e156) and aroa-htra double deletion mutant vaccine strain (s30) of salmonella enterica subsp. enterica serovar abortusequi under in vivo and in vitro growth conditions. | in the present study, cell lysate and cell supernatant of the both strains i.e., virulent wild type (e156) and mutant (s30) vaccine strains of salmonella enterica subspecies enterica serovar abortusequi (s. abortusequi), grown under varied in vivo and in vitro conditions were subjected to sds page and western blotting (using rabbit hyperimmune serum). variation in growth conditions did not have any significant effect on expression of different proteins. sds page of e156 and s30 cell lysate (cl) ... | 2008 | 18949890 |