Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| isolation of salmonella enterica serotype newport from a partly ruptured splenic abscess in a traveler returning from zanzibar. | salmonella enterica subsp. enterica serotype newport is a pathogen of growing importance because of its epidemic spread in dairy cattle and increasing rate of antimicrobial resistance. human infections, however, are rare. we report a case of a splenic abscess in a young traveler returning from east africa. | 2007 | 17634302 |
| complete nucleotide sequence of a small qnrs1-carrying plasmid from salmonella enterica subsp. enterica typhimurium dt193. | 2007 | 17652106 | |
| cytokine gene expression in chicken cecal tonsils following treatment with probiotics and salmonella infection. | probiotics are currently employed for control of pathogens and enhancement of immune response in chickens. in this study, we investigated the underlying immunological mechanisms of the action of probiotics against colonization of the chicken intestine by salmonella enterica subsp. enterica serovar typhimurium (salmonella serovar typhimurium). birds received probiotics by oral gavage on day 1 of age and, subsequently, received salmonella serovar typhimurium on day 2 of age. cecal tonsils were rem ... | 2008 | 17681719 |
| multiple-locus variable-number tandem repeat analysis of salmonella enterica subsp. enterica serovar typhimurium: comparison of isolates from pigs, poultry and cases of human gastroenteritis. | pulsed-field gel electrophoresis (pfge) and variable number tandem repeat (vntr) profiles of 195 epidemiologically unrelated salmonella typhimurium strains isolated in 1997-2004 from pigs were analysed and the results compared to establish the discriminatory ability of each method. in order to investigate the epidemiology of s. typhimurium from different populations, the vntr profiles from pigs were compared with those obtained from 190 s. typhimurium strains isolated from poultry and 186 strain ... | 2007 | 17714389 |
| oral sodium chlorate, topical disinfection, and younger weaning age reduce salmonella enterica shedding in pigs. | salmonella enterica subsp. enterica can cause swine illness or human foodborne disease. although nontoxic to mammalian cells, chlorate can be converted to cytotoxic chlorite by salmonellae. to test whether chlorate is effective at reducing salmonella shedding in weaned pigs exposed to shedding dams, a chlorate-nitrate-lactate (chlorate) oral dose was administered daily for 5 days following weaning, and this treatment was evaluated in combination with two weaning ages and a topical disinfectant. ... | 2007 | 17803134 |
| the role of roof rats ( rattus rattus) in the spread of salmonella enteritidis and s. infantis contamination in layer farms in eastern japan. | the prevalence of salmonella in four layer farms in eastern japan was investigated between 2004 and 2006 to determine the role of roof rats (rattus rattus) in the epizootology of salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis). persistent s. enteritidis and s. infantis contamination of the environment and pooled egg samples were detected in three out of four layer farms. a total of 113 (13.3%) and 158 (18.6%) out of 851 rats examined were positive for s. enteritidis and ... | 2008 | 17988423 |
| phenolic acids from plant foods can increase or decrease the mutation frequency to antibiotic resistance. | phenolic compounds are ubiquitous in plant foods, and they have been reported to have multiple biological effects. this study investigates the ability of derivatives of hydroxybenzoic and hydroxycinnamic acids to influence the development of ciprofloxacin resistance in the antibiotic-sensitive strain salmonella enterica subsp. enterica serovar typhimurium. we have found that cinnamic acid and its hydroxy derivatives increase the frequency of mutations leading to ciprofloxacin resistance in compa ... | 2007 | 17994694 |
| variable number tandem repeat typing of bacteria. | analysis of bacterial genomes revealed a high percentage of dna consisting of repeats, in which dna motifs existed in multiple copies. study of these dna motifs has resulted in the development of variable number tandem repeat (vntr) or multilocus variant-repeat analysis (mlva) assays, which have shown to be valuable bacterial typing methods, especially in relation to disease outbreaks. the vntr-based assay is based on direct pcr amplification of a specific locus, which is well defined. the range ... | 2007 | 18025706 |
| longitudinal study of a clonal, subclinical outbreak of salmonella enterica subsp. enterica serovar cerro in a u.s. dairy herd. | salmonellae are a major group of foodborne pathogens known to affect both humans and animals. dairy cattle are a known reservoir of these bacteria and human salmonella infections have been associated with the consumption of improperly processed or contaminated dairy products. many of the over 2500 known serotypes of salmonella are known to infect cattle, resulting in asymptomatic to fatal salmonellosis. this study describes the course of a salmonella outbreak and subsequent endemic infection on ... | 2007 | 18041954 |
| multiple-locus variable-number tandem-repeats analysis of listeria monocytogenes using multicolour capillary electrophoresis and comparison with pulsed-field gel electrophoresis typing. | the multiple-locus variable-number tandem-repeats analysis (mlva) method for genotyping has proven to be a fast and reliable typing tool in several bacterial species. mlva is in our laboratory the routine typing method for salmonella enterica subsp. enterica serovar typhimurium and escherichia coli o157. the gram-positive bacteria listeria monocytogenes, while not isolated as frequent as s. typhimurium and e. coli, causes severe illness with an overall mortality rate of 30%. thus, it is importan ... | 2008 | 18096258 |
| characterization of multiple-antimicrobial-resistant salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry in malaysia. | the aims of this communication were to study characterization of serogroups among salmonella isolates and the relationship of antimicrobial resistance to serogroups. multiple antimicrobial resistance (mar) was performed on 189 salmonella enterica isolates associated with 38 different serovars that were recovered from poultry and four types of indigenous vegetables. | 2008 | 18179445 |
| induction of the carrier state in pigeons infected with salmonella enterica subspecies enterica serovar typhimurium pt99 by treatment with florfenicol: a matter of pharmacokinetics. | paratyphoid caused by salmonella enterica subsp. enterica serovar typhimurium is the main bacterial disease in pigeons. the ability of salmonella serovar typhimurium to persist intracellularly inside pigeon macrophages results in the development of chronic carriers, which maintain the infection in the flock. in this study, the effect of drinking-water medication with florfenicol on salmonella infection in pigeons was examined. the pharmacokinetics of florfenicol in pigeons revealed a relatively ... | 2008 | 18180355 |
| toll-like receptor 5-deficient mice have dysregulated intestinal gene expression and nonspecific resistance to salmonella-induced typhoid-like disease. | the recognition of flagellin by toll-like receptor 5 (tlr5) is the dominant means by which model intestinal epithelia activate proinflammatory gene expression in response to salmonella enterica. the role of the flagellin-tlr5 interaction in vivo has been addressed primarily via studies that use flagellar mutants. such studies suggest that host recognition of flagellin promotes rapid neutrophil recruitment that protects the host from this pathogen. however, these works do not directly address the ... | 2008 | 18195036 |
| viability of zoonotic pathogens escherichia coli and salmonella in swine manure slurries with and without a urease inhibitor and thymol. | to determine the effects of urine, a urease inhibitor and/or an odour-reducing antimicrobial compound, on zoonotic pathogens in swine manure slurries. | 2008 | 18298450 |
| pyrosequencing analysis of the gyrb gene to differentiate bacteria responsible for diarrheal diseases. | pathogens causing acute diarrhea include a large variety of species from enterobacteriaceae and vibrionaceae. a method based on pyrosequencing was used here to differentiate bacteria commonly associated with diarrhea in china; the method is targeted to a partial amplicon of the gyrb gene, which encodes the b subunit of dna gyrase. twenty-eight specific polymorphic positions were identified from sequence alignment of a large sequence dataset and targeted using 17 sequencing primers. of 95 isolate ... | 2008 | 18324428 |
| hypoxia-independent activation of hif-1 by enterobacteriaceae and their siderophores. | hypoxia inducible factor-1 (hif-1) is the key transcriptional regulator during adaptation to hypoxia. recent studies provide evidence for hif-1 activation during bacterial infections. however, molecular details of how bacteria activate hif-1 remain unclear. here, we pursued the role of bacterial siderophores in hif-1 activation during infection with enterobacteriaceae. | 2008 | 18325389 |
| cloth-based hybridization array system for the identification of antibiotic resistance genes in salmonella. | a simple macroarray system based on the use of polyester cloth as the solid phase for dna hybridization has been developed for the identification and characterization of bacteria on the basis of the presence of various virulence and toxin genes. in this approach, a multiplex polymerase chain reaction (pcr) incorporating digoxigenin-dutp is used to simultaneously amplify different marker genes, with subsequent rapid detection of the amplicons by hybridization with an array of probes immobilized o ... | 2007 | 18363231 |
| ultrafiltration-based techniques for rapid and simultaneous concentration of multiple microbe classes from 100-l tap water samples. | this study focused on ultrafiltration as a technique for simultaneously concentrating and recovering viruses, bacteria and parasites in 100-l drinking water samples. a chemical dispersant, sodium polyphosphate, and tween 80 were used to increase microbial recovery efficiencies. secondary concentration was performed to reduce sample volumes to 3-5 ml for analysis using tissue culture, microscopy, and real-time pcr and rt-pcr. at seeding levels of 100-1000 (cfu, pfu, oocysts, or particles), a "hig ... | 2008 | 18395278 |
| real-time optical detection of methicillin-resistant staphylococcus aureus using lytic phage probes. | staphylococcus aureus (s. aureus)-specific bacteriophage was used as a probe for detection of methicillin-resistant s. aureus (mrsa) in aqueous solution using a novel optical method. biorecognition phage monolayers transferred to glass substrates using langmuir-blodgett (lb) technique were exposed individually to mrsa in solution at logarithmic concentrations ranging from 10(6) to 10(9)cfu/ml, and observed for real-time binding using a cytoviva optical light microscope system. results indicate t ... | 2008 | 18424125 |
| molecular fingerprinting of salmonella enterica subsp. enterica typhimurium and salmonella enterica subsp. enterica derby isolated from tropical seafood in south india. | salmonella enterica subsp. enterica typhimurium and salmonella enterica subsp. enterica derby strains isolated from different seafood were genotyped by pcr-ribotyping and eric-pcr assays. this study has ascertained the genetic relatedness among serovars prevalent in tropical seafood. pcr-ribotyping exhibited genetic variation in both salmonella serovars, and ribotype profile (ii) was most predominant, which was observed in 10/18 of salmonella enterica subsp. enterica typhimurium and 7/17 salmone ... | 2008 | 18480975 |
| multi-locus variable-number tandem repeat analysis for outbreak studies of salmonella enterica serotype enteritidis. | salmonella enterica subsp. enterica serotype enteritidis is known as an important and pathogenic clonal group which continues to cause worldwide sporadic cases and outbreaks in humans. here a new multiple-locus variable-number tandem repeat analysis (mlva) method is reported for highly-discriminative subtyping of salmonella enteritidis. emphasis was given on the most predominant phage types pt4 and pt8. the method comprises multiplex pcr specifically amplifying repeated sequences from nine diffe ... | 2008 | 18513386 |
| analysis of salmonella agona and salmonella weltevreden in malaysia by pcr fingerprinting and antibiotic resistance profiling. | forty-eight strains of salmonella enterica subsp. enterica serovar agona and 33 strains of salmonella enterica subsp. enterica serovar weltevreden were characterized by random amplified polymorphic dna (rapd) fingerprinting using 3 different arbitrary primer, enterobacterial repetitive intergenic consensus-polymerase chain reaction (eric-pcr) and antimicrobial susceptibility testing. by using rapd, 81 strains (44 strains of s. agona and 33 strains of s. weltevreden) can be clustered into 14 grou ... | 2008 | 18548329 |
| molecular analysis of multiresistant porcine salmonella enterica subsp. enterica serovar bredeney isolates from southern brazil: identification of resistance genes, integrons and a group ii intron. | the relationships of 83 porcine salmonella enterica subsp. enterica serovar bredeney isolates obtained at two slaughterhouses in southern brazil were analysed by xbai and blni macrorestriction analysis, plasmid profiling and determination of antimicrobial resistance patterns. twenty-nine xbai and 30 blni macrorestriction patterns were identified. the 72 plasmid-bearing isolates exhibited 20 different plasmid profiles. multiresistance was detected in 49 isolates (59%), of which 39 isolates showed ... | 2008 | 18571903 |
| persistence of salmonella senftenberg in poultry production environments and investigation of its resistance to desiccation. | most salmonella serovars, including salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg), are tolerant to desiccation and able to colonize and persist in feed mills. in addition, they may survive cleaning and disinfection procedures used on poultry farms. the present study was conducted to investigate the survival of s. senftenberg in broiler parent stock farms and broiler farms. the isolates from one of the parent stock farms investigated only differed by a single band in fl ... | 2008 | 18622860 |
| a retrospective case-control study of risk factors associated with salmonella enterica subsp. enterica serovar enteritidis infections on dutch broiler breeder farms. | the organized control of salmonella enterica subsp. enterica serovar enteritidis (se) in the dutch poultry sector aims to prevent vertical transmission by the top-down approach, but its effectiveness is now undermined by the increasing role of horizontal transmission, so that preventive hygiene on the poultry farm gains significant importance. in order to assess the actual level of preventive hygiene and to identify risk factors, an inquiry was held among a representative number of broiler breed ... | 1995 | 18645784 |
| subinhibitory concentrations of tetracycline affect virulence gene expression in a multi-resistant salmonella enterica subsp. enterica serovar typhimurium dt104. | treatment of salmonellosis with antibiotics is controversial and may prolong carriage and shedding. therefore, this study sought to investigate if exposure to antimicrobials influences the expression of factors involved in virulence and host colonization. the effect of subinhibitory tetracycline treatment (16 microg/ml, 30 min) on a multi-drug resistant salmonella typhimurium dt104 strain was investigated using a targeted microarray. real-time reverse transcriptase pcr was used to confirm and fu ... | 2008 | 18656407 |
| multiple-locus variable-number tandem repeat analysis for subtyping of salmonella enterica subsp. enterica serovar enteritidis. | salmonella enterica subsp. enterica serovar enteritidis is a major food-borne pathogen that caused most of salmonella infections worldwide. s. enteritidis phage type 4 (pt4) especially presents a real challenge for the classical typing methods. we developed a simple multiple-locus variable-number tandem repeat analysis (mlva) assay based on three hypervariable variable-number tandem repeat (vntr) loci for subtyping of salmonella enteritidis. testing an arbitrary chosen strain collection of 110 s ... | 2009 | 18706857 |
| the mechanism of ciprofloxacin resistance in dihydrogen peroxide-induced mutants of salmonella enterica subsp. enterica serovar typhimurium consists mainly in mutations in gyra gene and less in mutations affecting ciprofloxacin uptake. | the effect of h(2)o(2) on the induction of ciprofloxacin (cfl) resistant mutants of salmonella enterica subsp. enterica serovar typhimurium was evaluated and determinants of cfl resistance in the mutants were analyzed. factors associated with cfl resistance in h(2)o(2)-induced mutants included (i) mutations in gyra gene, predominantly (63 %) asp(87)-->asn and less (37 %) ser(83)-->phe substitutions, (ii) mutations in the regulatory genes of marrab or soxrs or in the individual structural genes o ... | 2008 | 18759123 |
| environmental sampling to predict fecal prevalence of salmonella in an intensively monitored dairy herd. | although dairy cattle are known reservoirs for salmonellae, cattle that are shedding this organism are often asymptomatic and difficult to identify. a dairy herd that was experiencing a sustained, subclinical outbreak of salmonella enterica subsp. enterica cerro was monitored for 2 years. fecal samples from the lactating cows were collected every 6 to 8 weeks and tested for the presence of salmonella. fecal prevalence of salmonella fluctuated throughout the observation period and ranged from 8 t ... | 2008 | 18939739 |
| comparative analysis of protein profiles of wild virulent (e156) and aroa-htra double deletion mutant vaccine strain (s30) of salmonella enterica subsp. enterica serovar abortusequi under in vivo and in vitro growth conditions. | in the present study, cell lysate and cell supernatant of the both strains i.e., virulent wild type (e156) and mutant (s30) vaccine strains of salmonella enterica subspecies enterica serovar abortusequi (s. abortusequi), grown under varied in vivo and in vitro conditions were subjected to sds page and western blotting (using rabbit hyperimmune serum). variation in growth conditions did not have any significant effect on expression of different proteins. sds page of e156 and s30 cell lysate (cl) ... | 2008 | 18949890 |
| rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry . | variations in the mass spectral profiles of multiple housekeeping proteins of 126 strains representing salmonella enterica subsp. enterica (subspecies i), s. enterica subsp. salamae (subspecies ii), s. enterica subsp. arizonae (subspecies iiia), s. enterica subsp. diarizonae (subspecies iiib), s. enterica subsp. houtenae (subspecies iv), and s. enterica subsp. indica (subspecies vi), and salmonella bongori were analyzed to obtain a phylogenetic classification of salmonellae based on whole-cell m ... | 2008 | 18952875 |
| development of liposome immunoassay for salmonella spp. using immunomagnetic separation and immunoliposome. | the ability to detect salmonella spp. is essential in the prevention of foodborne illness. this study examined a salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (ims/il) encapsulating sulforhodamine b (srb), a fluorescent dye. a quantitative assay was conducted by measuring the fluorescence intensity of srb that was produced from an immunomagnetic bead-salmonella spp.-immunoliposome complex. the results indicated detection limits of 2.7 ... | 2008 | 18955821 |
| poultry-associated salmonella enterica subsp. enterica serovar 4,12:d:- reveals high clonality and a distinct pathogenicity gene repertoire. | a european baseline survey during the years 2005 and 2006 has revealed that the monophasic salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in german broiler flocks. in denmark and the united kingdom, its serovar prevalences were 15.15% and 2.8%, respectively. although poultry is a major source of human salmonellosis, serovar 4,12:d:- is rarely isolated in humans (approximately 0.09% per year). molecular typing studies usi ... | 2009 | 19114530 |
| recovery of salmonella from bermudagrass exposed to simulated wastewater. | most confined swine (sus scrofa) feeding operations in the southeastern united states hold manure in lagoons and apply effluent on bermudagrass [cynodon dactylon (l.) pers.] as fertilizer. salmonella enterica subsp. enterica (ex kauffman and edwards) le minor and popoff, has been reported in mississippi lagoons, but levels and potential for contamination of bermudagrass were unknown. a laboratory method was developed to examine salmonella contamination of bermudagrass and levels of salmonella we ... | 2009 | 19141824 |
| importance of subunit vaccine antigen of major fli c antigenic site of salmonella enteritidis ii: a challenge trial. | salmonella enterica subsp. enterica serovar enteritidis (se) infection in chickens shows a mild pathogenicity except for young ages, compared with other animals, and laying hens sometimes produce se-contaminated eggs leading to public health concerns. to reduce the problem, se bacterin in poultry farms has been applied. we previously demonstrated that a subunit antigen, g.m. part polypeptide in se-fli c (sep 9), could be a candidate subunit antigen of se vaccine which may show less side effects ... | 2009 | 19186198 |
| distribution and genotypic characterization of salmonella serovars isolated from tropical seafood of cochin, india. | to determine the distribution of salmonella serovars in seafood and to examine the intraserovar genetic variations in salmonella enterica subsp. enterica serovar rissen and salmonella weltevreden by polymerase chain reaction (pcr)-ribotyping and enterobacterial repetitive intergenic consensus (eric)-pcr methods. | 2009 | 19200318 |
| risk factors for salmonella enterica subsp. enterica contamination in 519 french laying hen flocks at the end of the laying period. | a cross-sectional study was carried out to identify risk factors for salmonella spp. contamination in french laying hen flocks at the end of the laying period. five hundred and nineteen flocks were studied between october 2004 and september 2005. the salmonella status of the flocks was assessed from 5 faeces samples (pooled faeces samples from cage flocks and foot swabs from flocks kept on the floor, i.e. in a barn, outdoors and on organic farms) and 2 dust samples analysed using a classical bac ... | 2009 | 19237216 |
| emergence, distribution, and molecular and phenotypic characteristics of salmonella enterica serotype 4,5,12:i:-. | salmonella spp. represent one of the most common causes of bacterial foodborne illnesses around the world. the species salmonella enterica contains more than 2500 serotypes, and emergence of new human pathogenic salmonella strains and serotypes represents a major public health issue. salmonella enterica subsp. enterica serotype 4,5,12:i:- represents a monophasic variant of salmonella typhimurium, which has rarely been identified before the mid-1990 s. the prevalence of this serotype among human ... | 2009 | 19292687 |
| characterization of pathogenic and resistant genome repertoire reveals two clonal lines in salmonella enterica subsp. enterica serovar paratyphi b (+)-tartrate positive. | a total of 36 contemporary human, animal, and environmental (+)-tartrate-fermenting (dt+) salmonella enterica serovar paratyphi b isolates, formerly called salmonella serovar java, and five related monophasic s. enterica serovar 4,5,12:b:- isolates from belgium, germany, the netherlands, and the united kingdom were investigated for clonality and antimicrobial resistance profiles, as well as their virulence and resistance gene repertoire. two major clonal lines, which could be phenotypically diff ... | 2009 | 19292689 |
| salmonella enterica serovar enteritidis pathogenicity island 1 is not essential for but facilitates rapid systemic spread in chickens. | salmonella enterica subsp. enterica serovar enteritidis is a leading cause of human food-borne illness that is mainly associated with the consumption of contaminated poultry meat and eggs. to cause infection, s. enteritidis is known to use two type iii secretion systems, which are encoded on two salmonella pathogenicity islands, spi-1 and spi-2, the first of which is thought to play a major role in invasion and bacterial uptake. in order to study the role of spi-1 in the colonization of chicken, ... | 2009 | 19364835 |
| search for salmonella spp. in ostrich productive chain of brazilian southeast region. | we analyzed ostriches from an equipped farm located in the brazilian southeast region for the presence of salmonella spp. this bacterium was investigated in 80 samples of ostrich droppings, 90 eggs, 30 samples of feed and 30 samples of droppings from rodents. additionally, at slaughter-house this bacterium was investigated in droppings, caecal content, spleen, liver and carcasses from 90 slaughtered ostriches from the studied farm. also, blood serum of those animals were harvested and submitted ... | 2009 | 19390983 |
| effects of static magnetic field on cell growth, viability, and differential gene expression in salmonella. | in the present study, we investigated the effect of exposure to a static magnetic field (smf) on cell growth, viability, and gene expression of salmonella enterica subsp. enterica serovar hadar. our results indicated that smf exposure (200 mt, 13 hours) failed to alter cellular growth but induced a decrease of colony-forming units (cfu) between 3 and 6 hours followed by an increase from 6 to 9 hours. the analysis of the differential expression of rpoa, dnak, katn, and 16s rrna genes under smf ex ... | 2009 | 19422305 |
| analysis of antimicrobial resistance and plasmid profiles in salmonella serovars associated with tropical seafood of india. | a total of 256 salmonella strains consisting of 29 salmonella serovars isolated from seafood of cochin (india) were analyzed for resistance to antimicrobials commonly used in human and veterinary medicines as therapeutic agents. the 10 most predominant salmonella serovars in seafood were also characterized for presence of plasmids using the alkaline lysis method. antimicrobial susceptibility studies highlighted a comparatively high resistance in salmonella isolates to sulfamethizol and carbenici ... | 2009 | 19422307 |
| [characterisation of a phenotypic monophasic variant belonging to salmonella enterica subsp. enterica serovar typhimurium from wild birds and its possible transmission to cats and humans]. | in the last two years the national salmonella reference laboratory (nrl-salm) received an accumulating number of salmonellae with sero-formula 4,12:-:1,2 isolated from perished wild birds, particularly siskins. within these strains flagellar antigen of the first phase was phenotypically not detectable. by pcr a fragment could be amplified coding specifically for the h:i-flagellar antigen. consequently, this is a phenotypically monophasic variant of salmonella enterica subsp. enterica serovar typ ... | 2009 | 19517930 |
| dna microarray for molecular epidemiology of salmonella. | salmonellosis is a common infection estimated to affect 3 billion people and to cause 200,000 deaths every year. infections can appear as enteric fever, gastroenteritis, bacteremia, or extraintestinal focal infection. the course of the disease depends on a variety of factors, including infective dose, immune status of the host, and the genetic background of both the host and the pathogen. it has been recognized that certain salmonella types play a major role in the epidemiology of salmonella. he ... | 2009 | 19521880 |
| turtle-associated salmonella septicaemia and meningitis in a 2-month-old baby. | a severe case of reptile-associated salmonellosis which caused septicaemia and meningitis in a 2-month-old baby is reported. the infrequent serotype salmonella enterica subsp.(i) enterica serotype abony (4,5 : b : enx) was detected in the human sample as well as in the pet turtle's faeces. the importance of regulation and public awareness is highlighted. | 2009 | 19528160 |
| salmonella expressing a t-cell epitope from sendai virus are able to induce anti-infection immunity. | bacterial fimbriae can accept foreign peptides and display them on the cell surface. a highly efficient gene replacement method was used to generate peptide vaccines based on salmonella enterica subsp. enterica serovar typhimurium lt2. dna encoding an epitope from sendai virus, sv9 (sendai virus nucleoprotein peptide 324-332, fapgnypal), which is known to induce cytotoxic t lymphocytes, was incorporated into the gene encoding agfa (the major subunit protein of thin aggregative fimbriae of salmon ... | 2009 | 19528162 |
| reliable means of diagnosis and serovar determination of blood-borne salmonella strains: quick pcr amplification of unique genomic loci by novel primer sets. | typhoid fever is becoming an ever increasing threat in the developing countries. we have improved considerably upon the existing pcr-based diagnosis method by designing primers against a region that is unique to salmonella enterica subsp. enterica serovar typhi and salmonella enterica subsp. enterica serovar paratyphi a, corresponding to the sty0312 gene in s. typhi and its homolog spa2476 in s. paratyphi a. an additional set of primers amplify another region in s. typhi ct18 and s. typhi ty2 co ... | 2009 | 19535522 |
| virulotyping of salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in malaysia using multiplex-pcr. | the increased occurrence of salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. this study is aimed to detect the prevalence of twenty different virulence factors among salmonella enterica strains isolated from poultry and local indigenous vegetables in malaysia via an optimized, rapid and specific multiplex pcr assay. the assay encompasses a total of 19 salmonella pathogenicity islands genes and a quorum sensing gene (sdia) in three multiplex ... | 2009 | 19565351 |
| gamma radiation used as hygienization technique for foods does not induce viable but non-culturable state (vbnc) in salmonella enterica subsp. enterica serovar typhimurium. | gamma radiation has been widely used for hygienization of food products. whether gamma radiation stress induces vbnc state in salmonella is of great concern. therefore, the study was carried out to determine whether gamma radiation exposure induces vbnc state in salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium). the parameters tested were culturability on agar medium, transcriptional activity by rt-pcr, cytoplasmic membrane integrity, and direct viable count using live/dea ... | 2009 | 19641961 |
| o-antigen delays lipopolysaccharide recognition and impairs antibacterial host defense in murine intestinal epithelial cells. | although toll-like receptor (tlr) 4 signals from the cell surface of myeloid cells, it is restricted to an intracellular compartment and requires ligand internalization in intestinal epithelial cells (iecs). yet, the functional consequence of cell-type specific receptor localization and uptake-dependent lipopolysaccharide (lps) recognition is unknown. here, we demonstrate a strikingly delayed activation of iecs but not macrophages by wildtype salmonella enterica subsp. enterica sv. (s.) typhimur ... | 2009 | 19730692 |
| spi-7: salmonella's vi-encoding pathogenicity island. | salmonella pathogenicity island-7 (spi-7) is a large, mosaic, genetic island, found in several serovars of salmonella enterica subsp. enterica associated with systemic disease. as well as encoding genes which may aid its own transmission, it carries genes for potential virulence factors such as vi antigen, sope effector and type ivb pili. the stability of spi-7 is of interest with respect to typhoid fever and related vaccines. | 2008 | 19741287 |
| real-time fret pcr assay for salmonella enterica serotype detection in food. | salmonella enterica subsp. enterica serotypes are leading etiological agents of food-borne gastroenteritis. traditional identification is laborious and time intensive. faster molecular methods may allow early identification in contaminated food products. we developed a real-time, fluorescence resonance energy transfer hybridization probe polymerase chain reaction (pcr) assay for s. enterica serotypes on the basis of the exclusive presence of the apee gene in salmonella typhimurium. assay sensiti ... | 2009 | 19780376 |
| seroprevalence of antibodies against salmonella enterica subsp. enterica serovar gallinarum-pullorum in wild doves (zenaida asiatica and zenaida macroura) from the northeast of mexico. | a cross-sectional survey was conducted in order to determine the seroprevalence and to identify some factors associated with the presence of antibodies against salmonella enterica subsp. enterica serovar gallinarum-pullorum in white-winged (zenaida asiatica) and mourning doves (zenaida macroura) from hunting areas of northeast mexico. from september to october 2006, 201 serum samples were analyzed with the seroagglutination test. the overall seroprevalence of s. enterica subsp. enterica serovar ... | 2010 | 19800701 |
| infected abdominal aortic aneurysm caused by nontyphoid salmonella in an immunocompromised patient with rheumatoid arthritis. | nontyphoid salmonella strains are important pathogens commonly found worldwide, typically causing gastrointestinal illness. here, we report a case of a 66-yearold man with an abdominal aortic infected (or so-called mycotic) aneurysm caused by salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis). he had multiple risk factors for atherosclerosis: age over 60, a long history of smoking, an 8-year history of diabetes mellitus, and a 10-year history of rheumatoid arthritis treated ... | 2009 | 19856069 |
| the use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment. | in the present study, chromogenic (red) bacteria were used to simulate actual target bacteria during set-up and optimization of an isolation process of bacteria, designed for food samples. isolation of bacteria from food in the context of molecular biological detection of food pathogens is a multistep process. development of such a separation method requires continuous monitoring of the location of the presumable targets in the sample tubes. therefore, red-coloured pigmented bacteria were used a ... | 2010 | 19874486 |
| antimicrobial substances produced by coliform strains active against foodborne pathogens. | in the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in rio de janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. two strains (6.4%) exhibited inhibitory activity against the indicator strains, escherichia coli lmifrj and salmonella ente ... | 2010 | 19895262 |
| tracing lineage by phenotypic and genotypic markers in salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and salmonella typhimurium isolated in state of são paulo, brazil. | fifty-three salmonella 1,4,[5],12:i:- and 45 salmonella typhimurium strains were characterised using phage typing, plasmid profiles and pulsed-field gel electrophoresis (pfge) for comparison. the majority of the strains were subdivided into definitive type (dt) 41 (22.6%) and dt 193 (18%) and the 60-mda plasmid was detected in 94.3% and 84.4% of strains, respectively. genetic diversity was observed among all strains and 90% presented a > 70% similarity through pfge analysis. these results sugges ... | 2009 | 20027476 |
| virulotyping and antimicrobial resistance typing of salmonella enterica serovars relevant to human health in europe. | the combination of virulence gene and antimicrobial resistance gene typing using dna arrays is a recently developed genomics-based approach to bacterial molecular epidemiology. we have now applied this technology to 523 salmonella enterica subsp. enterica strains collected from various host sources and public health and veterinary institutes across nine european countries. the strain set included the five predominant salmonella serovars isolated in europe (enteritidis, typhimurium, infantis, vir ... | 2010 | 20039795 |
| prevalence and risk factors for salmonella enterica subsp. enterica contamination in french breeding and fattening turkey flocks at the end of the rearing period. | an epidemiological study was conducted to estimate the prevalence of salmonella spp. contamination in french commercial breeding and fattening turkey flocks at the end of the rearing period, as part of a european union-wide baseline study. two hundred and five breeding turkey flocks and three hundred and two fattening turkey flocks were included in the study, between october 2006 and september 2007. the salmonella status of flocks was assessed by collecting five environmental faeces samples, ana ... | 2010 | 20044159 |
| characterization of small cole1-like plasmids conferring kanamycin resistance in salmonella enterica subsp. enterica serovars typhimurium and newport. | multi-antibiotic resistant (mr) salmonella enterica serovars typhimurium and newport are an increasing concern in human and animal health. many strains are known to carry antibiotic resistance determinants on multiple plasmids, yet detailed information has been scarce. three plasmids conferring kanamycin (kan) resistance were isolated and nucleotide sequences were determined. two kan(r) plasmids from salmonella newport strains, psn11/00kan and psn02/01kan, were found to be identical and were 569 ... | 2010 | 20100512 |
| development and evaluation of a multiplex real-time polymerase chain reaction procedure to clinically type prevalent salmonella enterica serovars. | a multiplex real-time polymerase chain reaction procedure was developed to identify the most prevalent clinical isolates of salmonella enterica subsp. enterica. genes from the rfb, flic, fljb, and viab groups that encode the o, h, and vi antigens were used to design 15 primer pairs and taqman probes specific for the genes rfbj, wzx, flic, fljb, wcdb, the sdf-l sequence, and inva, which was used as an internal amplification control. the primers and probes were variously combined into six sets. th ... | 2010 | 20110454 |
| characterisation of pathogenic bacteria in a uasb-polishing pond system using molecular techniques. | molecular techniques have been commonly used to detect and quantify pathogenic bacteria in food, clinical and environmental samples, but in wastewater treatment plants few studies have been carried out. this work applied pcr with a specific set of primers to investigate pathogenic bacteria in a wastewater plant comprised of a uasb reactor followed by polishing ponds. in addition, in-situ hybridisation technique (fish) was used to estimate the abundance of escherichia coli in the system. accordin ... | 2010 | 20150719 |
| evaluation of pet-related management factors and the risk of salmonella spp. carriage in pet dogs from volunteer households in ontario (2005-2006). | the purpose of this study was to determine pet-related management factors that may be associated with the presence of salmonella spp. in feces of pet dogs from volunteer households. from october 2005 until may 2006, 138 dogs from 84 households in ontario were recruited to participate in a cross-sectional study. five consecutive daily fecal samples were collected from each dog and enrichment culture for salmonella spp. was performed. a higher than expected number of the dogs (23.2%; 32/138) had a ... | 2011 | 20163574 |
| autonomous growth of isolated single listeria monocytogenes and salmonella enterica serovar typhimurium cells in the absence of growth factors and intercellular contact. | the aim of this study was to observe growth of isolated single bacterial cells in the absence of growth factors and intercellular contact. in order to exclude stochastic uncertainties induced by dilution series, a new micromanipulation method was developed to ensure explicit results under visual control. this was performed with particular care for production of single prokaryotic cells and subsequent investigation of their autonomous growth. over 450 single isolated listeria monocytogenes and sa ... | 2010 | 20173058 |
| pulsed-field gel electrophoresis diversity of human and bovine clinical salmonella isolates. | pulsed-field gel electrophoresis (pfge) characterization of 335 temporally and spatially matched clinical, bovine, and human salmonella enterica subsp. enterica isolates revealed 167 xbai pfge patterns. these isolates were previously classified into 51 serotypes and 73 sequence types, as determined by multilocus sequence typing. discriminatory power of pfge (simpson's index, d = 0.991) was considerably higher than that of multilocus sequence typing (d = 0.920) or serotyping (d = 0.913). although ... | 2010 | 20180633 |
| [study on the epidemiological characteristics and molecular typing of salmonella enterica subsp. enterica serovar senftenberg in shanghai]. | to study the molecular epidemiological characteristics of salmonella enterica subsp. enterica serovar senftenberg (salmonella senftenberg) in shanghai, from 2006 to 2007. | 2009 | 20193231 |
| inactivation of salmonella enteritidis strains by combination of high hydrostatic pressure and nisin. | the effects of high hydrostatic pressure (hhp) and nisin treatment alone and in combination on cellular components and viability of two salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) strains were evaluated by differential scanning calorimetry (dsc) and plate counting in order to evaluate the relative resistance and optimize the treatment conditions. s. enteritidis fda and osu 799 strains were subjected to hhp (0.1-550 mpa for 10 min at 25 degrees c) alone and in combina ... | 2010 | 20226554 |
| effect of bovine lactoferrin in salmonella ser. typhimurium infection in mice. | lactoferrin (lf) has in vitro antimicrobial activity against gram-negative bacteria. salmonella enterica subsp. enterica serovar typhimurium causes systemic infection and acute diarrhea in humans, mainly in children younger than 2 years of age. the aim of the study was to determine the in vivo effect of bovine lf in salmonella ser. typhimurium infection in mice. 58 balb/c mice were employed. two hours before the infection with 300 microl of 10(7) cfu of salmonella ser. typhimurium, 29 mice recei ... | 2010 | 20306285 |
| dna microarray analysis of salmonella serotype typhimurium strains causing different symptoms of disease. | salmonella enterica subsp. enterica is one of the leading food-borne pathogens in the usa and european countries. outcome of human salmonella serotype typhimurium infections ranges from mild self-limiting diarrhoea to severe diarrhoea that requires hospitalization. increased knowledge of the mechanisms that are responsible for causing infection and especially the severity of infection is of high interest. | 2010 | 20356366 |
| a predominant multidrug-resistant salmonella enterica serovar saintpaul clonal line in german turkey and related food products. | recently, salmonella enterica subsp. enterica serovar saintpaul has increasingly been observed in several countries, including germany. however, the pathogenic potential and epidemiology of this serovar are not very well known. this study describes biological attributes of s. saintpaul isolates obtained from turkeys in germany based on characterization of their pheno- and genotypic properties. fifty-five s. saintpaul isolates from german turkeys and turkey-derived food products isolated from 200 ... | 2010 | 20363784 |
| proteins from latex of calotropis procera prevent septic shock due to lethal infection by salmonella enterica serovar typhimurium. | the latex of calotropis procera has been used in traditional medicine to treat different inflammatory diseases. the anti-inflammatory activity of latex proteins (lp) has been well documented using different inflammatory models. in this work the anti-inflammatory protein fraction was evaluated in a true inflammatory process by inducing a lethal experimental infection in the murine model caused by salmonella enterica subsp. enterica serovar typhimurium. | 2010 | 20371281 |
| investigation of an outbreak of salmonella enterica subsp. enterica serovar hadar food illness in the abruzzi region of italy. | a comparative study was made of 22 strains of salmonella hadar isolated from victims of an outbreak of food illness in the abruzzi region of italy in 2000 and 21 strains of the same serotype isolated from poultry meat and human stool samples in the abruzzi and molise regions between 2000 and 2001. the aim of the investigation was to provide an epidemiological interpretation of the food illness outbreak to establish the degree of similarity between the s. hadar strains isolated from victims of th ... | 2008 | 20405442 |
| serotypes and antimicrobial resistance profiles of salmonella isolates from pigs at slaughter in kenya. | salmonellosis is considered one of the most widespread food-borne zoonoses in industrialized as well as developing countries. the presence of salmonella in food animals at slaughter and the consequent cross-contamination of edible carcass tissues present a significant food safety hazard. | 2010 | 20440063 |
| pork contaminated with salmonella enterica serovar 4,[5],12:i:-, an emerging health risk for humans. | salmonella enterica subsp. enterica serovar 4,[5],12:i:- is a monophasic variant of s. enterica serovar typhimurium (antigenic formula 4,[5],12:i:1,2). worldwide, especially in several european countries and the united states, it has been reported among the 10 most frequently isolated serovars in pigs and humans. in the study reported here, 148 strains of the monophasic serovar isolated from pigs, pork, and humans in 2006 and 2007 in germany were characterized by various phenotypic and genotypic ... | 2010 | 20472721 |
| salmonella enterica serovar enteritidis tatb and tatc mutants are impaired in caco-2 cell invasion in vitro and show reduced systemic spread in chickens. | salmonella enterica subsp. enterica serovar enteritidis is a leading causative agent of gastroenteritis in humans. this pathogen also colonizes the intestinal tracts of poultry and can spread systemically in chickens. transfer to humans usually occurs through undercooked or improperly handled poultry meat or eggs. the bacterial twin-arginine transport (tat) pathway is responsible for the translocation of folded proteins across the cytoplasmic membrane. in order to study the role of the tat syste ... | 2010 | 20498258 |
| evaluation of antimicrobial activity of probiotic bacteria against salmonella enterica subsp. enterica serovar typhimurium 1344 in a common medium under different environmental conditions. | the importance of probiotics in human nutrition has been gaining recognition in recent years. these organisms have been shown to promote human health by enhancing immunological and digestive functions and fighting respiratory tract infections. we propose an improved in vitro model for the study of probiotic antimicrobial activity against enteropathogens, by attempting to re-create, in a common culture medium, environmental growth conditions comparable to those present in the small intestine. a p ... | 2010 | 20600855 |
| attachment and colonization by escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium, and staphylococcus aureus on stone fruit surfaces and survival through a simulated commercial export chain. | the ability of the foodborne pathogens escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium, and staphylococcus aureus to attach, colonize, and survive on stone fruit surfaces was investigated. fifty microliters of bacterial suspension was spot inoculated onto the sterile intact fructoplane of whole peaches and plums. minimum time required for initial adhesion and attachment was recorded for different surface contact times. surface colonizatio ... | 2010 | 20615337 |
| the prevalence of multidrug resistance is higher among bovine than human salmonella enterica serotype newport, typhimurium, and 4,5,12:i:- isolates in the united states but differs by serotype and geographic region. | salmonella represents an important zoonotic pathogen worldwide, but the transmission dynamics between humans and animals as well as within animal populations are incompletely understood. we characterized salmonella isolates from cattle and humans in two geographic regions of the united states, the pacific northwest and the northeast, using three common subtyping methods (pulsed-field gel electrophoresis [pfge], multilocus variable number of tandem repeat analysis [mlva], and multilocus sequence ... | 2010 | 20639364 |
| the o28 antigen gene clusters of salmonella enterica subsp. enterica serovar dakar and serovar pomona are different. | a 10 kb o-antigen gene cluster was sequenced from a salmonella enterica subsp. enterica dakar o28 reference strain and from two s. pomona serogroup o28 isolates. the two s. pomona o antigen gene clusters showed only moderate identity with the s. dakar o28 gene cluster, suggesting that the o antigen oligosaccharides may contain one or more sugars conferring the o28 epitope but may otherwise be different. these novel findings are absolutely critical for the correct interpretation of molecular sero ... | 2010 | 20652070 |
| association between phylogeny, virulence potential and serovars of salmonella enterica. | salmonella enterica subsp. enterica is one of the leading causes of zoonotic food-borne disease worldwide. the consequence of these infections is a serious impact on economics of the society in the form of lost productivity and expenses for medical care. the objective of this study was to analyze the difference in genomic content between selected serovars, especially the content of pathogenicity genes and this was done with a dna microarray. furthermore, we investigated the phylogenetic relation ... | 2010 | 20656064 |
| salmonella enterica subsp. enterica in cattle egret (bubulcus ibis) chicks from central texas: prevalence, serotypes, pathogenicity, and epizootic potential. | cattle egrets have a worldwide distribution, feed in proximity to cattle and other domestic animals, and often nest in large colonies in urban woodlots. over a 3-yr period, nestlings from five cattle egret colonies from central texas, usa, were surveyed for salmonellosis. prevalence of infection ranged from 29% to 95%. seventeen salmonella enterica subsp. enterica serotypes were isolated, of which the 4,5,12:i-monophasic serotype predominated in cultures of both the digestive tract and pooled sp ... | 2010 | 20688631 |
| optical genetic mapping defines regions of chromosomal variation in serovars of s. enterica subsp. enterica of concern for human and animal health. | summaryinfections involving salmonella enterica subsp. enterica serovars have serious animal and human health implications; causing gastroenteritis in humans and clinical symptoms, such as diarrhoea and abortion, in livestock. in this study an optical genetic mapping technique was used to screen 20 field isolate strains from four serovars implicated in disease outbreaks. the technique was able to distinguish between the serovars and the available sequenced strains and group them in agreement wit ... | 2010 | 20860874 |
| high-throughput universal probe salmonella serotyping (upss) by nanopcr. | salmonella enterica subsp. enterica serovar identification is of great importance with respect to outbreak monitoring and case verification. therefore rapid, sensitive and cost efficient detection of salmonella spp. is indispensable within microbiology labs. to amalgamate single tube isolate identification with salmonella typing, we developed the high-throughput universal probe salmonella serotyping (upss) technique based on nano liter pcr. in comparison to the classical approach, where o- and h ... | 2010 | 20869995 |
| the evolution and distribution of phage st160 within salmonella enterica serotype typhimurium. | summarysalmonellosis is an internationally important disease of mammals and birds. unique epidemics in new zealand in the recent past include two salmonella serovars: salmonella enterica subsp. enterica serovar typhimurium definitive type (dt) 160 (s. typhimurium dt160) and s. brandenburg. although not a major threat internationally, in new zealand s. typhimurium dt160 has been the most common serovar isolated from humans, and continues to cause significant losses in wildlife. we have identified ... | 2010 | 20950514 |
| rapid multiple-locus variable-number tandem-repeats analysis of shigella spp. using multicolour capillary electrophoresis. | the multiple-locus variable-number tandem-repeats analysis (mlva) method for genotyping has proven to be a fast and reliable typing tool in several bacterial species. mlva is in our laboratory the routine typing method for salmonella enterica subsp. enterica serovar typhimurium, escherichia coli (two assays), listeria monocytogenes and yersinia enterocolitica. the gram-negative invasive enteropathogenic bacterium shigella is the most common cause of bacillary dysentery (shigellosis) worldwide, a ... | 2010 | 20951171 |
| differential identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum based on polymorphic regions of glgc and spec genes. | salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum cause fowl typhoid and pullorum disease in avian species, respectively, and have been of considerable economic importance to the poultry industry in parts of the world. the definitive diagnosis of these diseases can be made only by isolation and identification of the causative agent. however, rapid identification of biovars gallinarum and pullorum is not easily feasible due to their common antigenic structure ... | 2010 | 21111918 |
| chronic and acute infection of the gall bladder by salmonella typhi: understanding the carrier state. | despite major treatment and prevention efforts, millions of new typhoid infections occur worldwide each year. for a subset of infected individuals, salmonella enterica subsp. enterica serovar typhi colonizes the gall bladder and remains there long after symptoms subside, serving as a reservoir for the further spread of the disease. in this progress article, we explore recent advances in our understanding of the mechanisms by which salmonella spp.--predominantly s. typhi--colonize and persist in ... | 2010 | 21113180 |
| a subtractively optimized dna microarray using non-sequenced genomic probes for the detection of food-borne pathogens. | in this study, we present the successful detection of food-borne pathogens using randomly selected non-sequenced genomic dna probes-based dna microarray chips. three food-borne pathogens, staphylococcus aureus, salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium), and bacillus cereus, were subjected for the preparation of the dna microarray probes. initially, about 50 dna probes selected randomly from non-sequenced genomic dna of each pathogen were prepared by using a set of ... | 2010 | 21116735 |
| structures of the sep22 dodecamer, a dps-like protein from salmonella enterica subsp. enterica serovar enteritidis. | the crystal structure of sep22, a dna-binding protein from starved cells from salmonella enterica subsp. enterica serovar enteritidis, has been determined in two forms: the native state at 1.25 å resolution and an iron-soaked form at 1.30 å resolution. the sep22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. along the threefold axes of the 23 symmetry, hydrophilic asp channels that consist of asp ... | 2010 | 21206015 |
| efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees c. | an amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ... | 2011 | 21217035 |
| tola mediates the differential detergent resistance pattern between the salmonella enterica subsp. enterica serovars typhi and typhimurium. | the tol-pal genes are essential for maintaining the outer membrane integrity and detergent resistance in various gram-negative bacteria, including salmonella. the role of tola has been well established for the bile resistance of salmonella enterica subsp. enterica serovar typhimurium. we compared the bile resistance pattern between the s. enterica serovars typhi and typhimurium and observed that typhi is more resistant to bile-mediated damage. a closer look revealed a significant difference in t ... | 2011 | 21252278 |
| validation of a seminested pcr approach for rapid detection of salmonella enterica subsp. enterica serovar gallinarum. | salmonella enterica subsp. enterica serovar gallinarum (s. gallinarum) is the causative agent of fowl typhoid, one of the major causes of mortality and morbidity on poultry farms. even though it has been substantially eradicated in many developed countries, the disease still remains endemic in central and south america, in africa and in the mediterranean countries of europe. this leads to the routine screening of flocks, mainly by cultivation and serological techniques, which are expensive, as w ... | 2011 | 21256889 |
| resistance to ampicillin, third-generation cephalosporins, ciprofloxacin, co-trimoxazole and azithromycin in clinical isolates of salmonella enterica subsp. enterica from germany: real problem or sporadic circumstance? | the objective of this study was to determine the in vitro activity of ampicillin, third-generation cephalosporins, ciprofloxacin, co-trimoxazole and azithromycin against salmonella enterica isolates. none of the isolates tested showed resistance to third-generation cephalosporins or azithromycin. the rates of resistance to ampicillin, co-trimoxazole and ciprofloxacin were 16.8%, 3.2% and 0.8%, respectively. moreover, 7.2% of the isolates showed reduced ciprofloxacin susceptibility, but none of t ... | 2011 | 21265586 |
| change in attachment of salmonella typhimurium, yersinia enterocolitica, and listeria monocytogenes to pork skin and muscle after hot water and lactic acid decontamination. | the attachment of salmonella enterica subsp. enterica serovar typhimurium, yersinia enterocolitica, and listeria monocytogenes to pig skin and muscle tissue decontaminated with 80 °c water or 55 °c, 1% lactic acid for 5 and 15s was investigated. attachment properties differed between skin and muscle surfaces. a significantly higher number of firmly attached bacteria was found on the decontaminated skin surface compared to the non-treated skin surface, both on hot water (p<0.0001) and on lactic a ... | 2010 | 21269717 |
| geographical and temporal dissemination of salmonellae isolated from domestic animal hosts in the culiacan valley, mexico. | the prevalence and diversity of salmonellae from domestic animal hosts were investigated in the culiacan valley, mexico. a total of 240 farm animal feces (cows, chicken, and sheep) were evaluated for salmonella spp. presence from july 2008 to june 2009. salmonella enterica subsp. enterica strains were isolated from 76 samples (31.7%), and 20 serotypes were identified being salmonella oranienburg (25%), salmonella give (14%), salmonella saintpaul (12%), and salmonella minnesota (11%) the most fre ... | 2011 | 21274525 |
| novel virulence gene and clustered regularly interspaced short palindromic repeat (crispr) multilocus sequence typing scheme for subtyping of the major serovars of salmonella enterica subsp. enterica. | salmonella enterica subsp. enterica is the leading cause of bacterial food-borne disease in the united states. molecular subtyping methods are powerful tools for tracking the farm-to-fork spread of food-borne pathogens during outbreaks. in order to develop a novel multilocus sequence typing (mlst) scheme for subtyping the major serovars of s. enterica subsp. enterica, the virulence genes ssel and fimh and clustered regularly interspaced short palindromic repeat (crispr) loci were sequenced from ... | 2011 | 21278266 |
| genome of a european fresh-vegetable food safety outbreak strain of salmonella enterica subsp. enterica serovar weltevreden. | the genome of salmonella enterica subsp. enterica serovar weltevreden strain 2007-60-3289-1 was sequenced. the genome sequence of this fresh-vegetable isolate from scandinavia will be useful for the elucidation of plant host factors in comparison to other serovars of s. enterica subsp. enterica. | 2011 | 21296964 |
| rapid identification of salmonella enterica serovars, typhimurium, choleraesuis, infantis, hadar, enteritidis, dublin and gallinarum, by multiplex pcr. | salmonella enterica subsp. enterica poses a threat to both human and animal health, with more than 2500 serovars having been reported to date. salmonella serovars are identified by slide and tube agglutination tests using o and h antigen-specific anti-sera, although this procedure is both labor intensive and time consuming. establishment of a method for rapid screening of the major salmonella serovars is therefore required. we have established multiplex polymerase chain reaction (m-pcr) assays f ... | 2011 | 21329737 |
| prevalence of salmonella spp. antibodies to toxoplasma gondii, and newcastle disease virus in feral pigeons (columba livia) in the city of jaboticabal, brazil. | the rock pigeon (columba livia) may serve as a reservoir for several pathogenic agents that can be transmitted to poultry, wildlife, domesticated pets, and/or humans via excreta, secretions, or dust from feathers. in addition, ingestion of infected pigeons by wild and domestic animals can also transmit these pathogenic agents. the health status of 126 free-living pigeons in an urban area was evaluated by microbiologic culture for salmonella and serologic testing for the presence of antibodies fo ... | 2010 | 21370639 |