| a highly specific microrna-mediated mechanism silences ltr retrotransposons of strawberry. | small rnas are involved in a plethora of functions in plant genomes. in general, transcriptional gene silencing is mediated by 24-nucleotide sirnas and is required for maintaining transposable elements in a silenced state. however, micrornas are not commonly associated with transposon silencing. in this study, we performed small rna transcriptome and degradome analyses of the rosaceae model plant fragaria vesca (the woodland strawberry) at the genome-wide level, and identified mirna families and ... | 2016 | 26611654 |
| genetic dissection of the (poly)phenol profile of diploid strawberry (fragaria vesca) fruits using a nil collection. | over the last few years, diploid strawberry (fragaria vesca) has been recognized as a model species for applied research of cultivated strawberry (fragaria × ananassa) that is one of the most economically important crops. berries, particularly strawberries, are known for their high antioxidant capacity due to a high concentration of (poly) phenolic compounds. studies have already characterized the phenolic composition of fruits from sets of cultivated strawberries but the quantification of pheno ... | 2016 | 26566833 |
| the content of secondary metabolites and antioxidant activity of wild strawberry fruit (fragaria vesca l.). | chemical analyses carried out in 2011-2013 aimed at evaluating the contents of flavonoids, free phenolic acids, tannins, anthocyanins, and antioxidant activity (%) by means of dpph radical neutralization ability in fresh and air-dried fruits of three wild strawberry cultivars. examinations revealed differences in contents of biologically active substances determined in raw versus dried material depending on the cultivar. mean concentrations of flavonoids and tannins were highest in raw fruits of ... | 2015 | 26539306 |
| the effect of fragaria vesca extract on smear layer removal: a scanning electron microscopic evaluation. | successful endodontic treatment depends on elimination of the microorganisms through chemomechanical debridement. the aim of this in vitro study was to evaluate the effectiveness of fragaria vesca (wild strawberry) extract (fve) on the removal of smear layer (sl). | 2015 | 26526069 |
| comparative analysis of polygalacturonase in the fruit of strawberry cultivars. | the role of polygalacturonase (pg) in the development, ripening, and softening of fruit from two strawberry cultivars with different flesh firmness and softening characteristics was compared. changes in pg activity and gene expression during development, ripening and softening were measured. the pg genes from each cultivar were cloned and analyzed, and were classified with other pg genes using phylogenetic analysis. in toyonoka fruit, pg activity increased gradually, reaching a peak during the p ... | 2015 | 26505428 |
| live substrate positively affects root growth and stolon direction in the woodland strawberry, fragaria vesca. | studies of clonal plant foraging generally focus on growth responses to patch quality once rooted. here we explore the possibility of true plant foraging; the ability to detect and respond to patch resource status prior to rooting. two greenhouse experiments were conducted to investigate the morphological changes that occur when individual daughter ramets of fragaria vesca (woodland strawberry) were exposed to air above live (non-sterilized) or dead (sterilized) substrates. contact between daugh ... | 2015 | 26483826 |
| multilocus sex determination revealed in two populations of gynodioecious wild strawberry, fragaria vesca subsp. bracteata. | gynodioecy, the coexistence of females and hermaphrodites, occurs in 20% of angiosperm families and often enables transitions between hermaphroditism and dioecy. clarifying mechanisms of sex determination in gynodioecious species can thus illuminate sexual system evolution. genetic determination of gynodioecy, however, can be complex and is not fully characterized in any wild species. we used targeted sequence capture to genetically map a novel nuclear contributor to male sterility in a self-pol ... | 2015 | 26483011 |
| global identification and analysis of long non-coding rnas in diploid strawberry fragaria vesca during flower and fruit development. | long non-coding rnas (lncrnas) are a new class of regulatory molecules with roles in diverse biological processes. while much effort has been invested in the analysis of lncrnas from established plant models arabidopsis, maize, and rice, almost nothing is known about lncrnas from fruit crops, including those in the rosaceae family. | 2015 | 26481460 |
| identification, isolation, and expression analysis of heat shock transcription factors in the diploid woodland strawberry fragaria vesca. | heat shock transcription factors (hsfs) are known to play dominant roles in plant responses to heat, as well as other abiotic or biotic stress stimuli. while the strawberry is an economically important fruit plant, little is known about the hsf family in the strawberry. to explore the functions of strawberry hsfs in abiotic and biotic stress responses, this study identified 17 hsf genes (fvhsfs) in a wild diploid woodland strawberry (fragaria vesca, 2n = 2x = 14) and isolated 14 of these genes. ... | 2015 | 26442049 |
| acylphloroglucinol biosynthesis in strawberry fruit. | phenolics have health-promoting properties and are a major group of metabolites in fruit crops. through reverse genetic analysis of the functions of four ripening-related genes in the octoploid strawberry (fragaria × ananassa), we discovered four acylphloroglucinol (apg)-glucosides as native fragaria spp. fruit metabolites whose levels were differently regulated in the transgenic fruits. the biosynthesis of the apg aglycones was investigated by examination of the enzymatic properties of three re ... | 2015 | 26169681 |
| novel and recently evolved microrna clusters regulate expansive f-box gene networks through phased small interfering rnas in wild diploid strawberry. | the wild strawberry (fragaria vesca) has recently emerged as an excellent model for cultivated strawberry (fragaria × ananassa) as well as other rosaceae fruit crops due to its short seed-to-fruit cycle, diploidy, and sequenced genome. deep sequencing and parallel analysis of rna ends were used to identify f. vesca micrornas (mirnas) and their target genes, respectively. thirty-eight novel and 31 known mirnas were identified. many known mirnas targeted not only conserved mrna targets but also de ... | 2015 | 26143249 |
| high resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of rosa. | rosaceae is a family containing many economically important fruit and ornamental species. although fluorescence in situ hybridization (fish)-based physical mapping of plant genomes is a valuable tool for map-based cloning, comparative genomics and evolutionary studies, no studies using high resolution physical mapping have been performed in this family. previously we proved that physical mapping of single-copy genes as small as 1.1 kb is possible on mitotic metaphase chromosomes of rosa wichuran ... | 2015 | 26134672 |
| convergent evolution at the gametophytic self-incompatibility system in malus and prunus. | s-rnase-based gametophytic self-incompatibility (gsi) has evolved once before the split of the asteridae and rosidae. this conclusion is based on the phylogenetic history of the s-rnase that determines pistil specificity. in rosaceae, molecular characterizations of prunus species, and species from the tribe pyreae (i.e., malus, pyrus, sorbus) revealed different numbers of genes determining s-pollen specificity. in prunus only one pistil and pollen gene determine gsi, while in pyreae there is one ... | 2015 | 25993016 |
| using rna-seq to assemble a rose transcriptome with more than 13,000 full-length expressed genes and to develop the wagrhsnp 68k axiom snp array for rose (rosa l.). | in order to develop a versatile and large snp array for rose, we set out to mine ests from diverse sets of rose germplasm. for this rna-seq libraries containing about 700 million reads were generated from tetraploid cut and garden roses using illumina paired-end sequencing, and from diploid rosa multiflora using 454 sequencing. separate de novo assemblies were performed in order to identify single nucleotide polymorphisms (snps) within and between rose varieties. snps among tetraploid roses were ... | 2015 | 25954285 |
| development and preliminary evaluation of a 90 k axiom® snp array for the allo-octoploid cultivated strawberry fragaria × ananassa. | a high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry fragaria × ananassa. short-read sequences from one diploid and 19 octoploid accessions were aligned to the diploid fragaria vesca 'hawaii 4' reference genome to identify single nucleotide polymorphisms (snps) and indels for incorporation into a 90 k affymetrix® axiom® array. we report the development and preliminary evaluation of this array. | 2015 | 25886969 |
| a near-isogenic line (nil) collection in diploid strawberry and its use in the genetic analysis of morphologic, phenotypic and nutritional characters. | first near-isogenic line collection in diploid strawberry, a tool for morphologic, phenotypic and nutritional qtl analysis. diploid strawberry (fragaria vesca), with a small genome, has a high degree of synteny with the octoploid cultivated strawberry (f. × ananassa), so can be used as a simplified model for genetic analysis of the octoploid species. agronomically interesting traits are usually inherited quantitatively and they need to be studied in large segregating progenies well characterized ... | 2015 | 25841354 |
| sublethal effects of imidacloprid on interactions in a tritrophic system of non-target species. | imidacloprid is one of the most used insecticides worldwide, but is highly toxic to non-target arthropods. effects of sublethal imidacloprid intoxication can potentially propagate in food webs, yet little is known about the impact on non-target populations and communities. we investigated short-term sublethal toxicity of imidacloprid in a tritrophic model system of wild strawberry fragaria vesca, wood cricket nemobius sylvestris and nursery web spider pisaura mirabilis. strawberries were treated ... | 2015 | 25840341 |
| species-specific duplications driving the recent expansion of nbs-lrr genes in five rosaceae species. | disease resistance (r) genes from different rosaceae species have been identified by map-based cloning for resistance breeding. however, there are few reports describing the pattern of r-gene evolution in rosaceae species because several rosaceae genome sequences have only recently become available. | 2015 | 25759136 |
| strawberry homologue of terminal flower1 integrates photoperiod and temperature signals to inhibit flowering. | photoperiod and temperature are major environmental signals affecting flowering in plants. although molecular pathways mediating these signals have been well characterized in the annual model plant arabidopsis, much less information is known in perennials. many perennials including the woodland strawberry (fragaria vesca l.) are induced to flower in response to decreasing photoperiod and temperature in autumn and they flower following spring. we showed earlier that, in contrast with arabidopsis, ... | 2015 | 25720985 |
| environmental responses of the ft/tfl1 gene family and their involvement in flower induction in fragaria × ananassa. | flowering time control is important for fruit production in fragaria × ananassa. the flowering inhibition pathway has been extensively elucidated in the woodland strawberry, fragaria vesca, whereas the factors involved in its promotion remain unclear. in this study, we investigated the environmental responses of f. × ananassa ft and tfl1-like genes, which are considered key floral promoters and repressors in many plants, respectively. a putative floral promoter, faft3, was up-regulated in the sh ... | 2015 | 25666540 |
| re-annotation of the woodland strawberry (fragaria vesca) genome. | fragaria vesca is a low-growing, small-fruited diploid strawberry species commonly called woodland strawberry. it is native to temperate regions of eurasia and north america and while it produces edible fruits, it is most highly useful as an experimental perennial plant system that can serve as a model for the agriculturally important rosaceae family. a draft of the f. vesca genome sequence was published in 2011 [nat genet 43:223,2011]. the first generation annotation (version 1.1) were develope ... | 2015 | 25623424 |
| rna-seq analysis and transcriptome assembly for blackberry (rubus sp. var. lochness) fruit. | there is an increasing interest in berries, especially blackberries in the diet, because of recent reports of their health benefits due to their high content of flavonoids. a broad range of genomic tools are available for other rosaceae species but these tools are still lacking in the rubus genus, thus limiting gene discovery and the breeding of improved varieties. | 2015 | 25608670 |
| genome-wide identification and comparative analysis of the heat shock transcription factor family in chinese white pear (pyrus bretschneideri) and five other rosaceae species. | heat shock transcription factors (hsfs), which act as important transcriptional regulatory proteins in eukaryotes, play a central role in controlling the expression of heat-responsive genes. at present, the genomes of chinese white pear ('dangshansuli') and five other rosaceae fruit crops have been fully sequenced. however, information about the hsfs gene family in these rosaceae species is limited, and the evolutionary history of the hsfs gene family also remains unresolved. | 2015 | 25604453 |
| effects of high co₂ levels on fermentation, peroxidation, and cellular water stress in fragaria vesca stored at low temperature in conditions of unlimited o₂. | to better understand the tolerance of strawberries (fragaria vesca l.) to high co2 in storage atmospheres, fermentation and cellular damage were investigated. fruits were stored for 3 and 6 days at 0 °c in the presence of different co2 levels (0, 20, or 40%) with 20% o2. changes in pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) gene expression and in fermentative metabolites, as well as in bound water and malondialdehyde (mda) concentrations, were analyzed. in strawberries stored w ... | 2015 | 25568930 |
| engineering the anthocyanin regulatory complex of strawberry (fragaria vesca). | the woodland strawberry, fragaria vesca is a model fruit for a number of rosaceous crops. we have engineered altered concentrations of anthocyanin in f. vesca, to determine the impact on plant growth and fruit quality. anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the r2r3 myb activator, myb10. in contrast, a potential bhlh partner for myb10 (bhlh33) did not affect the anthocyanin pathway when knocked down using rnai constructs. metaboli ... | 2014 | 25477896 |
| dihydroflavonol 4-reductase genes encode enzymes with contrasting substrate specificity and show divergent gene expression profiles in fragaria species. | during fruit ripening, strawberries show distinct changes in the flavonoid classes that accumulate, switching from the formation of flavan 3-ols and flavonols in unripe fruits to the accumulation of anthocyanins in the ripe fruits. in the common garden strawberry (fragaria×ananassa) this is accompanied by a distinct switch in the pattern of hydroxylation demonstrated by the almost exclusive accumulation of pelargonidin based pigments. in fragaria vesca the proportion of anthocyanins showing one ... | 2014 | 25393679 |
| bioactivity of fragaria vesca leaves through inflammation, proteasome and autophagy modulation. | fragaria vesca leaves have been used in folk medicine for the treatment of several diseases, namely gastrointestinal, cardiovascular and urinary disorders, which could be related with the potential anti-inflammatory properties of the extract. this work aims to disclose the bioactivity and the underlying action mechanism of an extract from fragaria vesca leaves in order to support its traditional uses. | 2014 | 25305515 |
| efficient screening of long terminal repeat retrotransposons that show high insertion polymorphism via high-throughput sequencing of the primer binding site. | retrotransposons have been used frequently for the development of molecular markers by using their insertion polymorphisms among cultivars, because multiple copies of these elements are dispersed throughout the genome and inserted copies are inherited genetically. although a large number of long terminal repeat (ltr) retrotransposon families exist in the higher eukaryotic genomes, the identification of families that show high insertion polymorphism has been challenging. here, we performed an eff ... | 2014 | 25072847 |
| expression of a functional jasmonic acid carboxyl methyltransferase is negatively correlated with strawberry fruit development. | the volatile metabolite methyl jasmonate (meja) plays an important role in intra- and interplant communication and is involved in diverse biological processes. in this study, we report the cloning and functional characterization of a s-adenosyl-l-methionine:jasmonic acid carboxyl methyltransferase (jmt) from fragaria vesca and fragaria×ananassa. biochemical assays and comprehensive transcript analyses showed that jmt has been erroneously annotated as gene fusion with a carboxyl methyltransferase ... | 2014 | 25046752 |
| effect of calcium on strawberry fruit flavonoid pathway gene expression and anthocyanin accumulation. | two diploid woodland strawberry (fragaria vesca) inbred lines, ruegen f7-4 (red fruit-bearing) and yw5af7 (yellow fruit-bearing) were used to study the regulation of anthocyanin biosynthesis in fruit. ruegen f7-4 fruit had similar total phenolics and anthocyanin contents to commercial octoploid (f. × ananassa) cultivar seascape, while yw5af7 exhibited relatively low total phenolics content and no anthocyanin accumulation. foliar spray of cacl2 boosted fruit total phenolics content, especially an ... | 2014 | 25036468 |
| light quality regulates flowering in fvft1/fvtfl1 dependent manner in the woodland strawberry fragaria vesca. | control of flowering in the perennial model, the woodland strawberry (fragaria vesca l.), involves distinct molecular mechanisms that result in contrasting photoperiodic flowering responses and growth cycles in different accessions. the f. vesca homolog of terminal flower1 (fvtfl1) functions as a key floral repressor that causes short-day (sd) requirement of flowering and seasonal flowering habit in the sd strawberry. in contrast, perpetual flowering f. vesca accessions lacking functional fvtfl1 ... | 2014 | 24966865 |
| comparative analysis of secondary metabolites contents in fragaria vesca l. fruits. | fragaria vesca (wild strawberry) belongs to the rosaceae family. besides the leaves (fragariae folium) and roots (fragariae radix), the aromatic fruits (fragariae fructus) of wild strawberry are also herbal materials used in medicine. the aim of this study was to compare the value of phytochemical and antioxidant activity of wild strawberry fruits (fragaria vesca l.). the fruits were analyzed regarding their secondary metabolites contents (flavonoids, sum of phenolic acids, tannins, anthocyanins ... | 2014 | 24959786 |
| characterization of the lipoxygenase (lox) gene family in the chinese white pear (pyrus bretschneideri) and comparison with other members of the rosaceae. | lipoxygenases (loxs), a type of non-haem iron-containing dioxygenase, are ubiquitous enzymes in plants and participate in the formation of fruit aroma which is a very important aspect of fruit quality. amongst the various aroma volatiles, saturated and unsaturated alcohols and aldehydes provide the characteristic aroma of the fruit. these compounds are formed from unsaturated fatty acids through oxidation, pyrolysis and reduction steps. this biosynthetic pathway involves at least four enzymes, i ... | 2014 | 24906560 |
| floral transcriptomes in woodland strawberry uncover developing receptacle and anther gene networks. | flowers are reproductive organs and precursors to fruits and seeds. while the basic tenets of the abce model of flower development are conserved in angiosperms, different flowering plants exhibit different and sometimes unique characteristics. a distinct feature of strawberry (fragaria spp.) flowers is the development of several hundreds of individual apocarpous (unfused) carpels. these individual carpels are arranged in a spiral pattern on the subtending stem tip, the receptacle. therefore, the ... | 2014 | 24828307 |
| physiological integration modifies δ15n in the clonal plant fragaria vesca, suggesting preferential transport of nitrogen to water-stressed offspring. | one of the most striking attributes of clonal plants is their capacity for physiological integration, which enables movement of essential resources between connected ramets. this study investigated the capacity of physiological integration to buffer differences in resource availability experienced by ramets of the clonal wild strawberry plant, fragaria vesca. specifically, a study was made of the responses of connected and severed offspring ramets growing in environments with different water ava ... | 2014 | 24769538 |
| transposon based activation tagging in diploid strawberry and monoploid derivatives of potato. | diploid strawberry and potato transformed with a transposon tagging construct exhibited either global (strawberry) or local transposition (potato). an activation tagged, compact-sized strawberry mutant overexpressed the gene adjacent to ds. as major fruit and vegetable crops, respectively, strawberry and potato are among the first horticultural crops with draft genome sequences. to study gene function, we examined transposon-tagged mutant strategies in model populations for both species, fragari ... | 2014 | 24728112 |
| somatic embryogenesis, tetraploidy, and variant leaf morphology in transgenic diploid strawberry (fragaria vesca subspecies vesca 'hawaii 4'). | the diploid (2n = 2x = 14) strawberry model plant fragaria vesca ssp. vesca 'hawaii 4' was employed for functional analysis of expressed dna sequences initially identified as being unique to fragaria and of unknown or poorly understood function. 'hawaii 4' is prominent in strawberry research due to its ease of agrobacterium-mediated transformation and regenerability, and its status as the source of the first complete strawberry genomic sequence. our studies of a set of transformants have documen ... | 2014 | 24418064 |
| auxin biosynthesis by the yucca6 flavin monooxygenase gene in woodland strawberry. | auxin has been regarded as the main signal molecule coordinating the growth and ripening of fruits in strawberry, the reference genomic system for rosaceae. the mechanisms regulating auxin biosynthesis in strawberry are largely elusive. recently, we demonstrated that two yucca genes are involved in flower and fruit development in cultivated strawberry. here, we show that the woodland strawberry (fragaria vesca l.) genome harbors nine loci for yucca genes and eight of them encode functional prote ... | 2014 | 24373096 |
| molecular genetics and genomics of the rosoideae: state of the art and future perspectives. | the rosoideae is a subfamily of the rosaceae that contains a number of species of economic importance, including the soft fruit species strawberry (fragaria ×ananassa), red (rubus idaeus) and black (rubus occidentalis) raspberries, blackberries (rubus spp.) and one of the most economically important cut flower genera, the roses (rosa spp.). molecular genetics and genomics resources for the rosoideae have developed rapidly over the past two decades, beginning with the development and application ... | 2014 | 26504527 |
| sgr: an online genomic resource for the woodland strawberry. | fragaria vesca, a diploid strawberry species commonly known as the alpine or woodland strawberry, is a versatile experimental plant system and an emerging model for the rosaceae family. an ancestral f. vesca genome contributed to the genome of the octoploid dessert strawberry (f. ×ananassa), and the extant genome exhibits synteny with other commercially important members of the rosaceae family such as apple and peach. to provide a molecular description of floral organ and fruit development at th ... | 2013 | 24364888 |
| computational identification of micrornas in the strawberry (fragaria x ananassa) genome sequence and validation of their precise sequences by mir-race. | in plants, micrornas (mirnas) play significant roles in post-transcriptional gene regulation and have been found to control many genes involved in different biological and metabolic processes. extensive studies were carried out to discover mirnas and analyze their functions in model plant species, such as in arabidopsis and rice that have been reported. in this research, we used bioinformatics to predict micrornas in an important strawberry rootstock cultivar to discover and validate precise seq ... | 2014 | 24333854 |
| profiling polyphenols of two diploid strawberry (fragaria vesca) inbred lines using uhplc-hrms(n.). | phenolic compounds in the fruits of two diploid strawberries (fragaria vesca f. semperflorens) inbred lines-ruegen f7-4 (a red-fruited genotype) and yw5af7 (a yellow-fruited genotype) were characterised using ultra-high-performance liquid chromatography coupled with tandem high-resolution mass spectrometry (uhplc-hrms(n)). the changes of anthocyanin composition during fruit development and between ruegen f7-4 and yw5af7 were studied. about 67 phenolic compounds, including taxifolin 3-o-arabinosi ... | 2014 | 24176345 |
| ethylene is involved in strawberry fruit ripening in an organ-specific manner. | the fruit of the strawberry fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. however, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. mining of the fragaria vesca genome allowed for the identification of the two main ethylene ... | 2013 | 24098047 |
| expression of fragaria vesca pip aquaporins in response to drought stress: pip down-regulation correlates with the decline in substrate moisture content. | pip aquaporin responses to drought stress can vary considerably depending on the isoform, tissue, species or level of stress; however, a general down-regulation of these genes is thought to help reduce water loss and prevent backflow of water to the drying soil. it has been suggested therefore, that it may be necessary for the plant to limit aquaporin production during drought stress, but it is unknown whether aquaporin down-regulation is gradual or triggered by a particular intensity of the str ... | 2013 | 24086403 |
| evolution of ellagitannin content and profile during fruit ripening in fragaria spp. | ellagitannins and ellagic acid conjugates are polyphenols present in the human diet, in particular strawberries (fragaria spp.). the first aim of this study was isolation and structural characterization of casuarictin and 3-o-methyl ellagic acid 3'-o-α-rhamnopyranoside, which were found to be abundant in fragaria spp., along with agrimoniin. the second aim was accurate profiling and quantification of 26 ellagitannins and ellagic acid conjugates in six fragaria x ananassa cultivars and two fragar ... | 2013 | 23992396 |
| eugenol production in achenes and receptacles of strawberry fruits is catalyzed by synthases exhibiting distinct kinetics. | eugenol is a volatile that serves as an attractant for pollinators of flowers, acts as a defense compound in various plant tissues, and contributes to the aroma of fruits. its production in a cultivated species such as strawberry (fragaria × ananassa), therefore, is important for the viability and quality of the fruit. we have identified and functionally characterized three strawberry complementary dnas (cdnas) that encode proteins with high identity to eugenol synthases from several plant speci ... | 2013 | 23983228 |
| survey of simple sequence repeats in woodland strawberry (fragaria vesca). | the use of simple sequence repeats (ssrs), or microsatellites, as genetic markers has become popular due to their abundance and variation in length among individuals. in this study, we investigated linkage groups (lgs) in the woodland strawberry (fragaria vesca) and demonstrated variation in the abundances, densities, and relative densities of mononucleotide, dinucleotide, and trinucleotide repeats. mononucleotide, dinucleotide, and trinucleotide repeats were more common than longer repeats in a ... | 2013 | 23979890 |
| genome-scale transcriptomic insights into early-stage fruit development in woodland strawberry fragaria vesca. | fragaria vesca, a diploid woodland strawberry with a small and sequenced genome, is an excellent model for studying fruit development. the strawberry fruit is unique in that the edible flesh is actually enlarged receptacle tissue. the true fruit are the numerous dry achenes dotting the receptacle's surface. auxin produced from the achene is essential for the receptacle fruit set, a paradigm for studying crosstalk between hormone signaling and development. to investigate the molecular mechanism u ... | 2013 | 23898027 |
| deep sequencing discovery of novel and conserved micrornas in wild type and a white-flesh mutant strawberry. | micrornas (mirnas) are small non-coding rnas that regulate gene expression by base pairing to mrna target sequences, and play crucial roles in plant development and stress responses. the knowledge on post-transcriptional regulation by mirnas in strawberry is rather limited so far. in order to understand the role of mirna in the molecular control during strawberry fruit development, small rna libraries were constructed from fruits at the turning stage of strawberry cultivar 'sachinoka' and its wh ... | 2013 | 23807373 |
| targeted sequence capture provides insight into genome structure and genetics of male sterility in a gynodioecious diploid strawberry, fragaria vesca ssp. bracteata (rosaceae). | gynodioecy is a sexual system wherein females coexist with hermaphrodites. it is of interest not only because male-sterile plants are advantageous in plant breeding but also because it can be a crucial step in the evolutionary transition to entirely separate sexes (dioecy) from a hermaphroditic ancestor. the gynodioecious diploid wild strawberry, fragaria vesca ssp. bracteata (rosaceae), is a member of a clade with both dioecious and cultivated species, making it an ideal model in which to study ... | 2013 | 23749450 |
| maternal sex effects and inbreeding depression under varied environmental conditions in gynodioecious fragaria vesca subsp. bracteata. | gynodioecy (coexistence of females and hermaphrodites) is a sexual system that occurs in numerous flowering plant lineages. thus, understanding the features that affect its maintenance has wide importance. models predict that females must have a seed fitness advantage over hermaphrodites, and this may be achieved via seed quality or quantity. females in a population of fragaria vesca subsp. bracteata, a long-lived gynodioecious perennial, do not demonstrate a seed quantity advantage, so this stu ... | 2013 | 23723257 |
| differential expression of flavonoid 3'-hydroxylase during fruit development establishes the different b-ring hydroxylation patterns of flavonoids in fragaria × ananassa and fragaria vesca. | flavonoid 3'-hydroxylase (f3'h) was studied for the first time in different fragaria species. the cdna clones isolated from unripe and ripe fruits of fragaria x ananassa (garden strawberry) and fragaria vesca (wild strawberry) showed high similarity (99% at the amino acid level) to the publically available f. vesca genome sequence and no significant differences could be identified between species and developmental stages of the fruits. in contrast, the genomic f3'h clones showed differences in t ... | 2013 | 23623754 |
| genome-wide characterization and linkage mapping of simple sequence repeats in mei (prunus mume sieb. et zucc.). | because of its popularity as an ornamental plant in east asia, mei (prunus mume sieb. et zucc.) has received increasing attention in genetic and genomic research with the recent shotgun sequencing of its genome. here, we performed the genome-wide characterization of simple sequence repeats (ssrs) in the mei genome and detected a total of 188,149 ssrs occurring at a frequency of 794 ssr/mb. mononucleotide repeats were the most common type of ssr in genomic regions, followed by di- and tetranucleo ... | 2013 | 23555708 |
| pfru, a single dominant locus regulates the balance between sexual and asexual plant reproduction in cultivated strawberry. | strawberry (fragaria sp.) stands as an interesting model for studying flowering behaviour and its relationship with asexual plant reproduction in polycarpic perennial plants. strawberry produces both inflorescences and stolons (also called runners), which are lateral stems growing at the soil surface and producing new clone plants. in this study, the flowering and runnering behaviour of two cultivated octoploid strawberry (fragaria × ananassa duch., 2n = 8× = 56) genotypes, a seasonal flowering ... | 2013 | 23554259 |
| construction of an integrated high density simple sequence repeat linkage map in cultivated strawberry (fragaria × ananassa) and its applicability. | the cultivated strawberry (fragaria × ananassa) is an octoploid (2n = 8x = 56) of the rosaceae family whose genomic architecture is still controversial. several recent studies support the aaa'a'bbb'b' model, but its complexity has hindered genetic and genomic analysis of this important crop. to overcome this difficulty and to assist genome-wide analysis of f. × ananassa, we constructed an integrated linkage map by organizing a total of 4474 of simple sequence repeat (ssr) markers collected from ... | 2013 | 23248204 |
| effects of extraction condition on structural features and anticoagulant activity of f. vesca l. conjugates. | from the air-dried wild strawberry (fragaria vesca l., family rosaceae) leaves five water-soluble glycoconjugates fv i-v by different extraction conditions have been isolated. effects of extraction steps/agents on chemical composition and anticoagulant activity of fv i-v were examined. dark brown f. vesca conjugates fv i-v were recovered in 4.5-8.4% yields, based on dry herb. isolates were composed of carbohydrate, phenolic and protein components. fv i-v displayed on hplc broad molecule-mass dis ... | 2013 | 23218362 |
| transcriptome database resource and gene expression atlas for the rose. | for centuries roses have been selected based on a number of traits. little information exists on the genetic and molecular basis that contributes to these traits, mainly because information on expressed genes for this economically important ornamental plant is scarce. | 2012 | 23164410 |
| dehydrin, alcohol dehydrogenase, and central metabolite levels are associated with cold tolerance in diploid strawberry (fragaria spp.). | the use of artificial freezing tests, identification of biomarkers linked to or directly involved in the low-temperature tolerance processes, could prove useful in applied strawberry breeding. this study was conducted to identify genotypes of diploid strawberry that differ in their tolerance to low-temperature stress and to investigate whether a set of candidate proteins and metabolites correlate with the level of tolerance. 17 fragaria vesca, 2 f. nilgerrensis, 2 f. nubicola, and 1 f. pentaphyl ... | 2013 | 23014928 |
| liquid chromatographic/electrospray ionization tandem mass spectrometric study of polyphenolic composition of four cultivars of fragaria vesca l. berries and their comparative evaluation. | high-performance liquid chromatography coupled with ion spray mass spectrometry in the tandem mode with both negative and positive ionization was used for investigating a variety of polyphenolic compounds in four genotypes of fragaria vesca berries. about 60 phenolic compounds belonging to the compound classes of phenolic acids, ellagitannins, ellagic acid derivatives, flavonols, monomeric and oligomeric flavanols, dihydrochalcones and anthocyanins were reported, providing for the first time a q ... | 2012 | 22972789 |
| evolution of the rdr1 tnl-cluster in roses and other rosaceous species. | the resistance of plants to pathogens relies on two lines of defense: a basal defense response and a pathogen-specific system, in which resistance (r) genes induce defense reactions after detection of pathogen-associated molecular patterns (pamps). in the specific system, a so-called arms race has developed in which the emergence of new races of a pathogen leads to the diversification of plant resistance genes to counteract the pathogens' effect. the mechanism of resistance gene diversification ... | 2012 | 22905676 |
| transposon tagging in diploid strawberry. | fragaria vesca was transformed with a transposon tagging construct harbouring amino terminally deleted maize transposase and egfp (ac element), nptii, camv 35s promoter (p35s) driving transposase and mannopine synthase promoter (pmas) driving egfp (ds element). of 180 primary transgenics, 48 were potential launch pads, 72 were multiple insertions or chimaeras, and 60 exhibited somatic transposition. t₁ progeny of 32 putative launch pads were screened by multiplex pcr for transposition. evidence ... | 2012 | 22845757 |
| increasing catechin and procyanindin accumulation in high-co2-treated fragaria vesca strawberries. | this paper deals with the impact of low temperature and high co2 levels on flavonols, proanthocyanidins, and anthocyanins, synthesized via branched pathways from common precursors, in strawberries (fragaria vesca l.). flavonoids were identified with q-tof equipment and quantified by hplc-quadrupole. proanthocyanins b1 and b3 accumulated in co2-treated strawberries, whereas in untreated (air) fruit, flavonoid production was redirected toward anthocyanin accumulation with a sharp decrease in catec ... | 2012 | 22783976 |
| molecular regulation of seed and fruit set. | seed and fruit set are established during and soon after fertilization and determine seed and fruit number, their final size and, hence, yield potential. these processes are highly sensitive to biotic and abiotic stresses, which often lead to seed and fruit abortion. here, we review the regulation of assimilate partitioning, including the potential roles of recently identified sucrose efflux transporters in seed and fruit set and examine the similarities of sucrose import and hydrolysis for both ... | 2012 | 22776090 |
| physical mapping of a pollen modifier locus controlling self-incompatibility in apricot and synteny analysis within the rosaceae. | s-locus products (s-rnase and f-box proteins) are essential for the gametophytic self-incompatibility (gsi) specific recognition in prunus. however, accumulated genetic evidence suggests that other s-locus unlinked factors are also required for gsi. for instance, gsi breakdown was associated with a pollen-part mutation unlinked to the s-locus in the apricot (prunus armeniaca l.) cv. 'canino'. fine-mapping of this mutated modifier gene (m-locus) and the synteny analysis of the m-locus within the ... | 2012 | 22481163 |
| construction of black (rubus occidentalis) and red (r. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach. | the genus rubus belongs to the rosaceae and is comprised of 600-800 species distributed world-wide. to date, genetic maps of the genus consist largely of non-transferable markers such as amplified fragment length polymorphisms. an f(1) population developed from a cross between an advanced breeding selection of rubus occidentalis (96395s1) and r. idaeus 'latham' was used to construct a new genetic map consisting of dna sequence-based markers. the genetic linkage maps presented here are constructe ... | 2012 | 22398438 |
| metabolite profiling reveals novel multi-level cold responses in the diploid model fragaria vesca (woodland strawberry). | winter freezing damage is a crucial factor in overwintering crops such as the octoploid strawberry (fragaria × ananassa duch.) when grown in a perennial cultivation system. our study aimed at assessing metabolic processes and regulatory mechanisms in the close-related diploid model woodland strawberry (fragaria vescal.) during a 10-days cold acclimation experiment. based on gas chromatography/time-of-flight-mass spectrometry (gc/tof-ms) metabolite profiling of three f. vesca genotypes, clear dis ... | 2012 | 22370221 |
| clarifying the identity of the main ellagitannin in the fruit of the strawberry, fragaria vesca and fragaria ananassa duch. | although the composition of strawberry fruit has been extensively studied, especially for the most abundant phenolic compounds, agrimoniin has never been univocally identified as one of the most abundant phenolic compounds in the fruit. in this study agrimoniin was isolated in the fruit of fragaria vesca and its structure characterized. furthermore, its presence was definitively established to be the main ellagitannin in both f. vesca and fragaria ananassa d. fruit. the presence of sanguiin h-6 ... | 2012 | 22339338 |
| chlorogenic acid, rutin and hyperoside content in fragaria vesca, f. viridis and f. moschata in lithuania. | in lithuania, two species of the genus fragaria l. (rosaceae), f. vesca l. and f. viridis weston, occur naturally in the wild and two others, f. moschata weston and f. × ananassa duchesne ex rozier are found escaped from cultivation. the main objective of this study was to establish the variation pattern in the content of chlorogenic acid, rutin and hyperoside in leaves and fruits of the native lithuanian species. in this work, the chemical polymorphisms of different fragaria species were studie ... | 2013 | 22324400 |
| strategies for transcriptome analysis in nonmodel plants. | even with recent reductions in sequencing costs, most plants lack the genomic resources required for successful short-read transcriptome analyses as performed routinely in model species. several approaches for the analysis of short-read transcriptome data are reviewed for nonmodel species for which the genome of a close relative is used as the reference genome. two approaches using a data set from phytophthora-challenged rubus idaeus (red raspberry) are compared. over 70000000 86-nt illumina rea ... | 2012 | 22301897 |
| computational identification of micrornas in strawberry expressed sequence tags and validation of their precise sequences by mir-race. | micrornas (mirnas) are small, endogenously expressed, nonprotein-coding rnas that regulate gene expression at the post-transcriptional level in both animals and plants through repressing translation or inducing mrna degradation. a comprehensive strategy to identify new mirna homologs by mining the repository of available strawberry expressed sequence tags (ests) was developed. by adopting a range of filtering criteria, we identified 11 potential mirnas belonging to 5 mirna families from 47 890 f ... | 2012 | 22287696 |
| flower and early fruit development in a diploid strawberry, fragaria vesca. | the diploid woodland strawberry, fragaria vesca, is being recognized as a model for the more complex octoploid commercial strawberry, fragaria × ananassa. f. vesca exhibits a short seed to seed cycle, can be easily transformed by agrobacteria, and a draft genome sequence has been published. these features, together with its similar flower structure, potentially make f. vesca a good model for studying the flower development of other members of the rosaceae family, which contains many economically ... | 2012 | 22198460 |
| functional characterization of gynodioecy in fragaria vesca ssp. bracteata (rosaceae). | gynodioecy is a phylogenetically widespread and important sexual system where females coexist with hermaphrodites. because dioecy can arise from gynodioecy, characterization of gynodioecy in close relatives of dioecious and sub-dioecious species can provide insight into this transition. thus, we sought to determine whether fragaria vesca ssp. bracteata, a close relative to f. chiloensis and f. virginiana, exhibits the functional and population genetic hallmarks of a gynodioecious species. | 2012 | 22052984 |
| the tfl1 homologue ksn is a regulator of continuous flowering in rose and strawberry. | flowering is a key event in plant life, and is finely tuned by environmental and endogenous signals to adapt to different environments. in horticulture, continuous flowering (cf) is a popular trait introduced in a wide range of cultivated varieties. it played an essential role in the tremendous success of modern roses and woodland strawberries in gardens. cf genotypes flower during all favourable seasons, whereas once-flowering (of) genotypes only flower in spring. here we show that in rose and ... | 2012 | 21895811 |
| metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway. | wild strawberry (fragaria vesca) fruit contains several important phenylpropene aroma compounds such as eugenol, but cultivated varieties are mostly devoid of them. we have redirected the carbon flux in cultivated strawberry (fragaria×ananassa) fruit from anthocyanin pigment biosynthesis to the production of acetates of hydroxycinnamyl alcohols, which serve as the precursors of the phenylpropenes, by downregulating the strawberry chalcone synthase (chs) via rnai-mediated gene silencing and, alte ... | 2011 | 21689779 |
| an autotetraploid linkage map of rose (rosa hybrida) validated using the strawberry (fragaria vesca) genome sequence. | polyploidy is a pivotal process in plant evolution as it increase gene redundancy and morphological intricacy but due to the complexity of polysomic inheritance we have only few genetic maps of autopolyploid organisms. a robust mapping framework is particularly important in polyploid crop species, rose included (2n = 4x = 28), where the objective is to study multiallelic interactions that control traits of value for plant breeding. from a cross between the garden, peach red and fragrant cultivar ... | 2011 | 21647382 |
| searching for gene flow from cultivated to wild strawberries in central europe. | experimental crosses between the diploid woodland strawberry (fragaria vesca l.) and the octoploid garden strawberry (f. × ananassa duch.) can lead to the formation of viable hybrids. however, the extent of such hybrid formation under natural conditions is unknown, but is of fundamental interest and importance in the light of the potential future cultivation of transgenic strawberries. a hybrid survey was therefore conducted in the surroundings of ten farms in switzerland and southern germany, w ... | 2011 | 21307039 |
| the genome of woodland strawberry (fragaria vesca). | the woodland strawberry, fragaria vesca (2n = 2x = 14), is a versatile experimental plant system. this diminutive herbaceous perennial has a small genome (240 mb), is amenable to genetic transformation and shares substantial sequence identity with the cultivated strawberry (fragaria × ananassa) and other economically important rosaceous plants. here we report the draft f. vesca genome, which was sequenced to ×39 coverage using second-generation technology, assembled de novo and then anchored to ... | 2011 | 21186353 |
| generation and analysis of ests from strawberry (fragaria xananassa) fruits and evaluation of their utility in genetic and molecular studies. | cultivated strawberry is a hybrid octoploid species (fragaria xananassa duchesne ex. rozier) whose fruit is highly appreciated due to its organoleptic properties and health benefits. despite recent studies on the control of its growth and ripening processes, information about the role played by different hormones on these processes remains elusive. further advancement of this knowledge is hampered by the limited sequence information on genes from this species, despite the abundant information av ... | 2010 | 20849591 |
| snp discovery and genetic mapping of t-dna insertional mutants in fragaria vesca l. | as part of a program to develop forward and reverse genetics platforms in the diploid strawberry [fragaria vesca l.; (2n = 2x = 14)] we have generated insertional mutant lines by t-dna mutagenesis using pcambia vectors. to characterize the t-dna insertion sites of a population of 108 unique single copy mutants, we utilized thermal asymmetric interlaced pcr (hitail-pcr) to amplify the flanking region surrounding either the left or right border of the t-dna. bioinformatics analysis of flanking seq ... | 2010 | 20349033 |
| identification of flowering genes in strawberry, a perennial sd plant. | we are studying the regulation of flowering in perennial plants by using diploid wild strawberry (fragaria vesca l.) as a model. wild strawberry is a facultative short-day plant with an obligatory short-day requirement at temperatures above 15 degrees c. at lower temperatures, however, flowering induction occurs irrespective of photoperiod. in addition to short-day genotypes, everbearing forms of wild strawberry are known. in 'baron solemacher' recessive alleles of an unknown repressor, seasonal ... | 2009 | 19785732 |
| the development and characterisation of a bacterial artificial chromosome library for fragaria vesca. | the cultivated strawberry fragaria xananassa is one of the most economically-important soft-fruit species. few structural genomic resources have been reported for fragaria and there exists an urgent need for the development of physical mapping resources for the genus. the first stage in the development of a physical map for fragaria is the construction and characterisation of a high molecular weight bacterial artificial chromosome (bac) library. | 2009 | 19772672 |
| search for a plant for phytoremediation--what can we learn from field and hydroponic studies? | the main aim of the study was to evaluate the strategies for coping with arsenic toxicity developed by the mine species (calamagrostis arundinacea, fragaria vesca, stachys sylvatica, and epilobium parviflorum), and to compare results obtained from plants exposed to arsenic present in contaminated soil (2000-3500 mg/kg dw) and in hydroponic solution (2 microm and 12 microm arsenate). here we report basic differences in plant responses to arsenic depending on growth conditions (hydroponic/soil) wi ... | 2009 | 19733893 |
| [agamospermy in outbred crosses of fragaria vesca l. (rosaceae)]. | the seed progeny obtained in european wood strawberry fragaria vesca l. (2n = 2x = 14) via outbred crosses proved to include matromorphic diploid plants, whose proportion varied from 10.0 to 21.0%. to allow their identification, the crosses were performed using recessive dihomozygotes (variety alexandria) as a maternal form and dominant dihomozygotes as a paternal form. generation of parthenogenetic plants in ourbred crosses with known genetic markers suggested pseudogamous agamospermy. the ques ... | 2009 | 19382689 |
| allopolyploidy in fragariinae (rosaceae): comparing four dna sequence regions, with comments on classification. | potential events of allopolyploidy may be indicated by incongruences between separate phylogenies based on plastid and nuclear gene sequences. we sequenced two plastid regions and two nuclear ribosomal regions for 34 ingroup taxa in fragariinae (rosaceae), and six outgroup taxa. we found five well supported incongruences that might indicate allopolyploidy events. the incongruences involved aphanes arvensis, potentilla miyabei, potentilla cuneata, fragaria vesca/moschata, and the drymocallis clad ... | 2009 | 19268709 |
| high diversity of arbuscular mycorrhizal fungi in a boreal herb-rich coniferous forest. | * here, the diversity of arbuscular mycorrhizal (am) fungi was determined in a boreal herb-rich coniferous forest in relation to environmental variables. * root samples of five plant species (fragaria vesca, galeobdolon luteum, hepatica nobilis, oxalis acetosella and trifolium pratense) were analysed from stands differing in age and forest management intensity. * thirty-four glomeromycota taxa (small-subunit ribosomal rna gene (ssu rdna) sequence groups) were detected from 90 root samples (911 c ... | 2008 | 18537886 |
| effects of physical connection and genetic identity of neighbouring ramets on root-placement patterns in two clonal species. | root-placement patterns were examined in the clonal species glechoma hederacea and fragaria vesca when grown with different types of neighbours. three different patterns were predicted as consequences of different types of interactions between roots: the avoidance pattern if root growth decreases in the presence of neighbouring roots; the intrusive pattern if root growth increases towards neighbouring roots; and the unresponsive pattern if root growth is unaffected by neighbouring roots. experim ... | 2007 | 17850258 |
| first comprehensive contribution to medical ethnobotany of western pyrenees. | an ethnobotanical and medical study was carried out in the navarre pyrenees, an area known both for its high biological diversity and its cultural significance. as well as the compilation of an ethnopharmacological catalogue, a quantitative ethnobotanical comparison has been carried out in relation to the outcomes from other studies about the pyrenees. a review of all drugs used in the area has also been carried out, through a study of the monographs published by the institutions and organizatio ... | 2007 | 17553138 |
| characterization of polyphenol oxidase and peroxidase and influence on browning of cold stored strawberry fruit. | polyphenol oxidase and peroxidase were extracted from two different varieties of strawberry fruit (fragaria x ananassa d, cv. 'elsanta' and fragaria vesca l, cv. 'madame moutot') and characterized using reliable spectrophotometric methods. in all cases, the enzymes followed michaelis-menten kinetics, showing different values of peroxidase kinetics parameters between the two cultivars: km = 50.68 +/- 2.42 mm ('elsanta') and 18.18 +/- 8.79 mm ('madame moutot') mm and vmax = 0.14 +/- 0.03 u/g ('els ... | 2007 | 17407312 |
| small-scale heterogeneity in soil quality influences photosynthetic efficiency and habitat selection in a clonal plant. | in clonal plants, internode connections allow translocation of photosynthates, water, nutrients and other substances among ramets. clonal plants form large systems that are likely to experience small-scale spatial heterogeneity. physiological and morphological responses of fragaria vesca to small-scale heterogeneity in soil quality were investigated, together with how such heterogeneity influences the placement of ramets. as a result of their own activities plants may modify the suitability of t ... | 2006 | 16987921 |
| growth onset, senescence, and reproductive development of meadow species in mesocosms exposed to elevated o3 and co2. | we studied the effects of elevated o3 (40-50 ppb) and co2 (+100 ppm) alone and in combination on the growth onset, relative chlorophyll meter values, and reproductive development of meadow species grown in ground-planted mesocosms using open-top chambers. the 3-year study was conducted in the summers of 2002-2004. elevated o3 decreased the early season coverage of plant communities and delayed the flowering of campanula rotundifolia and vicia cracca. the relative chlorophyll meter values of frag ... | 2007 | 16815612 |
| strawberry fruit protein with a novel indole-acyl modification. | achenes and receptacle tissue of fragaria vesca, l. cultivar yellow wonder were shown to contain conjugated indole-3-acetic acid (iaa) that was not soluble in organic solvents and yielded iaa after strong alkaline hydrolysis, suggestive of iaa attached to plant proteins. this solvent insoluble conjugated iaa accounted for between 0.4 and 4 ng of iaa per gram fresh weight of tissue in both achenes and receptacles. to investigate this strawberry conjugate class further, a polyclonal antibody was p ... | 2006 | 16683161 |
| influences of elevated ozone and carbon dioxide in growth responses of lowland hay meadow mesocosms. | we studied the effects of relatively low levels of o(3) (40-50 ppb) and co(2)-enrichment (+100 ppm) on a northern european lowland hay meadow during the summers 2002-2004 using open-top chambers (otcs) and ground-planted mesocosms. ozone reduced the aboveground biomass of the community (up to 40%), and four out of seven species (campanula rotundifolia, fragaria vesca, trifolium medium, vicia cracca) showed either significant growth reduction and/or visible injuries under elevated o(3). however, ... | 2006 | 16513235 |
| biosynthesis of mono- and sesquiterpenes in strawberry fruits and foliage: 2h labeling studies. | the biosynthesis of the monoterpene (s)-linalool and the sesquiterpene trans-(s)-nerolidol in fruits of fragaria x ananassa duch. cv. eros and florence and of the monoterpene (-)-alpha-pinene in fragaria vesca was investigated by in vivo feeding experiments with [5,5-2h2]mevalonic acid lactone (d2-mvl) and [5,5-2h2]-1-deoxy-d-xylulose (d2-dox). the feeding experiments indicate that (s)-linalool and trans-(s)-nerolidol in fragaria x ananassa duch. and (-)-alpha-pinene in f. vesca are exclusively ... | 2006 | 16478276 |
| the development of issr-derived scar markers around the seasonal flowering locus (sfl) in fragaria vesca. | fragaria vesca is a short-lived perennial with a seasonal-flowering habit. seasonality of flowering is widespread in the rosaceae and is also found in the majority of temperate polycarpic perennials. genetic analysis has shown that seasonal flowering is controlled by a single gene in f. vesca, the seasonal flowering locus ( sfl). here, we report progress towards the marker-assisted selection and positional cloning of sfl, in which three issr markers linked to sfl were converted to locus-specific ... | 2004 | 15292991 |
| development of microsatellite markers in fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping. | we have developed 21 new microsatellites in the model diploid perennial species fragaria vesca from an enriched genomic library developed using f. vesca 'ruegen'. the transferability of the primer pairs to other fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawberry fragaria x ananassa, whereas 24 (77%) amplified a product in 7 other diploid fragaria species. we analysed the allelic variation among 15 f. vesca accessions using the 21 microsa ... | 2004 | 15190360 |
| catalytic properties of alcohol acyltransferase in different strawberry species and cultivars. | the substrate specificity of alcohol acyltransferase (aat) enzymes from different strawberry varieties was studied. proteins with aat activity from fruits of fragaria x ananassa duch. cv. oso grande were purified to apparent homogeneity and used for kinetic studies with different straight-chain alcohols and acyl-coas. k(m) values obtained for oso grande enzyme with six different alcohols, using acetyl-coa as cosubstrate, decreased with increasing length of the alcohol chain. in similar experimen ... | 2002 | 12083878 |
| occurrence of graft-transmissible virus diseases of the strawberry in the czech republic. | strawberry virus diseases were monitored using a leaf graft bioassay in 17 cultivars of strawberry fragaria ananassa duchesne in the czech republic. fragaria vesca indicator clones revealed after grafting several symptoms of strawberry mottle, crinkle, vein banding, and mild yellow edge as well as of mixed virus infections. isometric virus-like particles (vlps) ranging from 21 to 50 nm in diameter and flexuous filamentous vlps (12 by 600-1400 nm) were observed by electron microscopy in negativel ... | 2001 | 11774893 |
| separation of tc-99 in soil and plant samples collected around the chernobyl reactor using a tc-selective chromatographic resin and determination of the nuclide by icp-ms | technetium (tc) is known to have high mobility in a soil-water system and also high bioavailability for plants, because the most stable form of tc in natural surface environment is thought to be tco4- which is highly soluble. the chemical form of tc, however, changes with environmental conditions. thus, it is necessary, for realistic assessment, to obtain transfer parameters, such as transfer factors, under natural conditions. however, it is difficult to obtain these parameters using global fall ... | 2000 | 10879840 |
| isolation and characterization of mrnas differentially expressed during ripening of wild strawberry (fragaria vesca l.) fruits. | wild strawberry (fragaria vesca l.) is an attractive model system for studying ripening in non-climacteric fruit, because of its small diploid genome, its short reproductive cycle, and its capacity for transformation. we have isolated eight ripening-induced cdnas from this species after differential screening of a cdna library. the predicted polypeptides of seven of the clones exhibit similarity to database protein sequences, including acyl carrier protein, caffeoyl-coa 3-o-methyltransferase, se ... | 1999 | 10092188 |
| expression analysis of a ripening-specific, auxin-repressed endo-1, 4-beta-glucanase gene in strawberry. | a cdna (cel1) encoding an endo-1,4-beta-glucanase (egase) was isolated from ripe fruit of strawberry (fragaria x ananassa). the deduced protein of 496 amino acids contains a presumptive signal sequence, a common feature of cell wall-localized egases, and one potential n-glycosylation site. southern- blot analysis of genomic dna from f. x ananassa, an octoploid species, and that from the diploid species fragaria vesca indicated that the cel1 gene is a member of a divergent multigene family. in fr ... | 1998 | 9847104 |