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[wood strawberry]. 1979259538
a new potexvirus associated with strawberry mild yellow edge disease.a physical map of cdna clones prepared from dsrna associated with the my-18 source of strawberry mild yellow edge (smye) was constructed and 854 nucleotides adjacent to the 3' poly(a) tail were sequenced. the larger open reading frame product of mr 25714 showed considerable amino acid homology to the coat protein cistrons of six potexviruses and two carlaviruses. a second product of mr 11216 encoded completely within the coat protein cistron, but in a different frame, has similarities to two pot ...19902191082
anti-ulcer activity of procyanidins preparation of water-soluble procyanidin-cimetidine complexes.the anti-ulcer properties of water-soluble procyanidins prepared by fermentation of tannins from fragaria vesca were studied. complexes of procyanidins and cimetidine were prepared. the procyanidins increased the water-solubility of the cimetidine and may prevent undesirable nitrosamine formation in the stomach as they block its cyanamide function.19892608691
procyanidins from the roots of fragaria vesca: characterization and pharmacological approach. 19883409389
effect of ecological conditions on structure of wild strawberry leaf. 19744280633
clustering of foodstuffs in food hypersensitivity. an inquiry study in pollen allergic patients.600 pollen allergic patients were questioned about hypersensitivity symptoms from various foodstuffs. the answers regarding one foodstuff were compared with those of other foodstuffs making 780 tables, from which the spearmen 's correlation coefficients were calculated. most of the combinations of foodstuffs showed statistically significant correlations. the highest value of the correlation coefficient was found for certain combinations: various nuts reciprocally as well as nuts combined with ap ...19846731203
[phytoperfusion of the bladder after adenomectomy].for continuous irrigation of the bladder after prostatic adenomectomy herb infusion (urtica dioica l. 12-15 g/l, hypericum perforatum l. 8-12 g/l. marticaria recutita l. 8-10 g/l, folia plantaginis majoris 7-10 g/l, herba millefolii 4-6 g/l, folia betula 3-5 g/l. artemisia vulgaris l. 1-2 g/l, folia fragaria vesca 3-4 g/l, water 11 maximum) was dripped through a thin suprapubic and urethral drainages. such phytoperfusion of the urinary bladder used in 22 patients reduced postoperative blood loss ...19958571476
infectious cdna clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the disease.a full-length in vivo infectious cdna clone of strawberry mild yellow edge-associated potexvirus (smyeav) was constructed and used to inoculate fragaria vesca 'alpine' seedlings and rubus rosifollus. both host plants could be infected using particle bombardment or agroinoculation, but not by mechanical inoculation. a method that used potted strawberry plants for particle bombardment resulted in high survival and infection rates. the plants developed systemic infection and virus particles were de ...19979292025
quarantine strawberry vein banding virus firstly detected in slovakia and serbia.strawberry vein banding virus (svbv) was detected by polymerase chain reaction (pcr) and dot-blot hybridisation in samples of cultivated strawberry plants originating from central slovakia and in samples of wild strawberry plants from south-eastern serbia in federal republic of yugoslavia (fry). this is the first finding of svbv in these countries as well as of svbv in wild strawberry plants in europe.19989770076
expression analysis of a ripening-specific, auxin-repressed endo-1, 4-beta-glucanase gene in strawberry.a cdna (cel1) encoding an endo-1,4-beta-glucanase (egase) was isolated from ripe fruit of strawberry (fragaria x ananassa). the deduced protein of 496 amino acids contains a presumptive signal sequence, a common feature of cell wall-localized egases, and one potential n-glycosylation site. southern- blot analysis of genomic dna from f. x ananassa, an octoploid species, and that from the diploid species fragaria vesca indicated that the cel1 gene is a member of a divergent multigene family. in fr ...19989847104
isolation and characterization of mrnas differentially expressed during ripening of wild strawberry (fragaria vesca l.) fruits.wild strawberry (fragaria vesca l.) is an attractive model system for studying ripening in non-climacteric fruit, because of its small diploid genome, its short reproductive cycle, and its capacity for transformation. we have isolated eight ripening-induced cdnas from this species after differential screening of a cdna library. the predicted polypeptides of seven of the clones exhibit similarity to database protein sequences, including acyl carrier protein, caffeoyl-coa 3-o-methyltransferase, se ...199910092188
isolation of tobacco necrosis virus from strawberry leaves in the czech republic.leaves of symptomless fragaria ananassa duch cv. cacanská raná were grafted onto fragaria vesca indicator clones. thirty-five of 72 grafted indicator plants developed leaf mottle symptoms. isometric virus-like particles were observed in purified preparations from symptomatic leaves of f. vesca. the latter were mechanically inoculated to herbaceous host plants. a virus was successfully purified from nicotiana occidentalis 37 b symptomatic plants by differential and sucrose density gradient centri ...199810358734
quantitative genetics of floral traits in a gynodioecious wild strawberry fragaria virginiana: implications for the independent evolution of female and hermaphrodite floral phenotypes.the independent evolution of floral phenotype is an important part of the process of gender specialization during the evolution of dioecy from hermaphroditism. however, we have little information on the genetic variation of floral traits in species with separate genders. gynodioecious species (co-occurrence of females and hermaphrodites) have a breeding system intermediate between hermaphroditism and complete separation of the sexes (dioecy) and thus can provide insight into the genetic architec ...199910651918
separation of tc-99 in soil and plant samples collected around the chernobyl reactor using a tc-selective chromatographic resin and determination of the nuclide by icp-mstechnetium (tc) is known to have high mobility in a soil-water system and also high bioavailability for plants, because the most stable form of tc in natural surface environment is thought to be tco4- which is highly soluble. the chemical form of tc, however, changes with environmental conditions. thus, it is necessary, for realistic assessment, to obtain transfer parameters, such as transfer factors, under natural conditions. however, it is difficult to obtain these parameters using global fall ...200010879840
characterization and complete nucleotide sequence of strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses.an isolate of strawberry mottle virus (smov) was transferred from fragaria vesca to nicotiana occidentalis and chenopodium quinoa by mechanical inoculation. electron micrographs of infected tissues showed the presence of isometric particles of approximately 28 nm in diameter. smov-associated tubular structures were also conspicuous, particularly in the plasmodesmata of c. quinoa. dsrna extraction of smov-infected n. occidentalis yielded two bands of 6.3 and 7.8 kbp which were cloned and sequence ...200211752720
occurrence of graft-transmissible virus diseases of the strawberry in the czech republic.strawberry virus diseases were monitored using a leaf graft bioassay in 17 cultivars of strawberry fragaria ananassa duchesne in the czech republic. fragaria vesca indicator clones revealed after grafting several symptoms of strawberry mottle, crinkle, vein banding, and mild yellow edge as well as of mixed virus infections. isometric virus-like particles (vlps) ranging from 21 to 50 nm in diameter and flexuous filamentous vlps (12 by 600-1400 nm) were observed by electron microscopy in negativel ...200111774893
catalytic properties of alcohol acyltransferase in different strawberry species and cultivars.the substrate specificity of alcohol acyltransferase (aat) enzymes from different strawberry varieties was studied. proteins with aat activity from fruits of fragaria x ananassa duch. cv. oso grande were purified to apparent homogeneity and used for kinetic studies with different straight-chain alcohols and acyl-coas. k(m) values obtained for oso grande enzyme with six different alcohols, using acetyl-coa as cosubstrate, decreased with increasing length of the alcohol chain. in similar experimen ...200212083878
root cooperation in a clonal plant: connected strawberries segregate roots.the ability to selectively avoid competition with members of the same clone should be highly advantageous but has not been demonstrated in plants. we found that physical connection between plants in a clone of the wild strawberry fragaria chiloensis induced them to segregate their roots, significantly increasing clonal performance. such increase in performance was not found when plants were grown in containers that artificially divided their rooting zones. there was no effect of connection in a ...200312647182
infectivity of recombinant strawberry vein banding virus dna.infectivity of the cloned dna genome of strawberry vein banding virus (svbv) was demonstrated by particle bombardment of 4-week-old strawberry (fragaria vesca l. var. uc-5) plants with gold particles coated with the putative full-length 7.9 kb viral dna. vein banding symptoms developed on 15 % of inoculated plants 6-7 weeks post-inoculation. an approximate 1.25-mer of the viral dna was cloned into the binary vector pcgn1547. particle bombardment of this construct into strawberry plants gave an i ...200312771404
multiplex rt-pcr detection of four aphid-borne strawberry viruses in fragaria spp. in combination with a plant mrna specific internal control.the principal aphid-borne viruses infecting strawberry (fragaria spp.) strawberry crinkle virus (scv), strawberry mild yellow edge virus (smyev), strawberry mottle virus (smov) and strawberry vein banding virus (svbv) can cause serious crop losses. in this paper, a multiplex reverse transcriptase polymerase chain reaction (rt-pcr) method is described for the simultaneous detection of all four viruses in combination with a plant mrna specific internal control which can be used as an indicator of ...200312880923
high variability and disomic segregation of microsatellites in the octoploid fragaria virginiana mill. (rosaceae).the objectives of the present study were to develop microsatellite markers for the wild strawberry, fragaria virginiana, to evaluate segregation patterns of microsatellite alleles in this octoploid species, and assess genetic variability at microsatellite loci in a wild population. a genomic library was screened for microsatellite repeats and several pcr primers were designed and tested. we also tested the use of heterologous primers and found that f. virginiana primers amplified products in cul ...200312908097
[genetic studies on diploid fragaria species. i. crossing between fragaria vesca and fragaria nilgerrensis, its influence on the morphology of the plant and the development of sex]. 195813648695
[antimicrobial properties of fragaria vesca and fragaria grandifolia]. 196213870933
[fragaria vesca as a cholagogue]. 196414255531
constraints on the evolution of males and sexual dimorphism: field estimates of genetic architecture of reproductive traits in three populations of gynodioecious fragaria virginiana.to understand how genetic constraints may limit the evolution of males and sexual dimorphism in a gynodioecious species, i conducted a quantitative genetic experiment in a gynodioecious wild strawberry, fragaria virginiana. i estimated and compared genetic parameters (narrow-sense heritabilities, between-trait and between-sex genetic correlations, as well as phenotypic and genetic variance-covariance matrices) in the two sex morphs from three populations grown in a common field garden. i measure ...200314575323
the defh9-iaam auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry.the defh9-iaam gene fusion which is expressed specifically in placenta/ovules and promotes auxin-synthesis confers parthenocarpic fruit development to eggplant, tomato and tobacco. transgenic defh9-iaam eggplants and tomatoes show increased fruit production due mainly to an improved fruit set. however, the weight of the fruits is also frequently increased.200415113427
development of microsatellite markers in fragaria, their use in genetic diversity analysis, and their potential for genetic linkage mapping.we have developed 21 new microsatellites in the model diploid perennial species fragaria vesca from an enriched genomic library developed using f. vesca 'ruegen'. the transferability of the primer pairs to other fragaria species was high; all 31 primer pairs produced amplicons in 3 accessions of the octoploid strawberry fragaria x ananassa, whereas 24 (77%) amplified a product in 7 other diploid fragaria species. we analysed the allelic variation among 15 f. vesca accessions using the 21 microsa ...200415190360
the development of issr-derived scar markers around the seasonal flowering locus (sfl) in fragaria vesca.fragaria vesca is a short-lived perennial with a seasonal-flowering habit. seasonality of flowering is widespread in the rosaceae and is also found in the majority of temperate polycarpic perennials. genetic analysis has shown that seasonal flowering is controlled by a single gene in f. vesca, the seasonal flowering locus ( sfl). here, we report progress towards the marker-assisted selection and positional cloning of sfl, in which three issr markers linked to sfl were converted to locus-specific ...200415292991
functional characterization of enzymes forming volatile esters from strawberry and banana.volatile esters are flavor components of the majority of fruits. the last step in their biosynthesis is catalyzed by alcohol acyltransferases (aats), which link alcohols to acyl moieties. full-length cdnas putatively encoding aats were isolated from fruit of wild strawberry (fragaria vesca) and banana (musa sapientum) and compared to the previously isolated saat gene from the cultivated strawberry (fragaria x ananassa). the potential role of these enzymes in fruit flavor formation was assessed. ...200415326278
gain and loss of fruit flavor compounds produced by wild and cultivated strawberry species.the blends of flavor compounds produced by fruits serve as biological perfumes used to attract living creatures, including humans. they include hundreds of metabolites and vary in their characteristic fruit flavor composition. the molecular mechanisms by which fruit flavor and aroma compounds are gained and lost during evolution and domestication are largely unknown. here, we report on processes that may have been responsible for the evolution of diversity in strawberry (fragaria spp) fruit flav ...200415522848
isolation and diversity analysis of resistance gene analogues (rgas) from cultivated and wild strawberries.degenerate oligonucleotide primers, designed based on conserved regions of nucleotide binding site (nbs) domains from previously cloned plant resistance genes, were used to isolate resistance gene analogues (rgas) from wild and cultivated strawberries. seven distinct families of rgas of the nbs-lrr type were identified from two related wild species, fragaria vesca and f. chiloensis, and six different fragaria x ananassa cultivars. with one exception (gav-3), the deduced amino acid sequences of s ...200415565466
plant genotype affects total antioxidant capacity and phenolic contents in fruit.we wanted to determine the total antioxidant capacity (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid equivalent antioxidant assay; teac) of different species and cultivars (strawberry and apple) and to characterize the teac among fruits of the same varieties (peach and apricot) but grafted on different rootstocks.200515723750
high-efficiency transformation of the diploid strawberry (fragaria vesca) for functional genomics.fragaria vesca l., a diploid (2n = 2x = 14) relative of the commercial octoploid strawberry, is an attractive model for functional genomics research in rosaceae. its small genome size, short reproductive cycle, and facile vegetative and seed propagation make f. vesca a promising candidate for forward and reverse genetics experiments. however, the lack of a high-efficiency transformation protocol required for systematic production of thousands of t-dna insertional mutant lines and high-throughput ...200616320068
controlled atmosphere storage of wild strawberry fruit (fragaria vesca l.).controlled atmosphere storage technology to extend the shelf life of "reina de los valles" wild strawberry fruit (fragaria vesca l.) was studied. fruits were stored at 3 degrees c for three weeks in different atmosphere compositions: 0.05% co2/21% o2 (air), 3% co2/18% o2, 6% co2/15% o2, 10% co2/11% o2, and 15% co2/6% o2. the effect of gas composition on soluble solids content, titrable acidity, ph, off-flavor, aroma volatiles, and consumer preference was monitored. the result showed that the 10% ...200616390182
biosynthesis of mono- and sesquiterpenes in strawberry fruits and foliage: 2h labeling studies.the biosynthesis of the monoterpene (s)-linalool and the sesquiterpene trans-(s)-nerolidol in fruits of fragaria x ananassa duch. cv. eros and florence and of the monoterpene (-)-alpha-pinene in fragaria vesca was investigated by in vivo feeding experiments with [5,5-2h2]mevalonic acid lactone (d2-mvl) and [5,5-2h2]-1-deoxy-d-xylulose (d2-dox). the feeding experiments indicate that (s)-linalool and trans-(s)-nerolidol in fragaria x ananassa duch. and (-)-alpha-pinene in f. vesca are exclusively ...200616478276
influences of elevated ozone and carbon dioxide in growth responses of lowland hay meadow mesocosms.we studied the effects of relatively low levels of o(3) (40-50 ppb) and co(2)-enrichment (+100 ppm) on a northern european lowland hay meadow during the summers 2002-2004 using open-top chambers (otcs) and ground-planted mesocosms. ozone reduced the aboveground biomass of the community (up to 40%), and four out of seven species (campanula rotundifolia, fragaria vesca, trifolium medium, vicia cracca) showed either significant growth reduction and/or visible injuries under elevated o(3). however, ...200616513235
induction of phenylalanine ammonia-lyase in strawberry leaf disks: action spectra and effects of wounding, sucrose, and light.the increase in phenylalanine ammonia-lyase (pal) activity in strawberry (fragaria vesca var. wsu-1232) leaf disks required wounding, sucrose, and light and was cycloheximide-sensitive. in injured leaves and in leaf disks, the highest pal activity was detected nearest the wounded tissues. without wounding, no increase in activity was observed when leaves were cultured in sucrose and light.the optimal concentration of sucrose for enzyme activity increase ranged from 0.15 m to 0.4 m. at the subopt ...197416658948
temperature and aging effects on leaf membranes of a cold hardy perennial, fragaria virginiana.the lipid composition of leaves of wild strawberry (fragaria virginiana duchesne) was analyzed throughout an annual growth cycle in the field. cellular hardiness to temperature stress was assessed concomitantly by a solute leakage technique. leaves were shown to be very sensitive to an applied temperature of -5 degrees c during the summer months but insensitive to a 35 degrees c treatment. this general pattern was also seen in young overwintering leaves but was reversed after a period of low-tem ...198116662118
effects of light and nutrients on leaf size, co(2) exchange, and anatomy in wild strawberry (fragaria virginiana).plants of a single genotype of wild strawberry, fragaria virginiana duchesne, were grown with or without fertilizer in high (406 microeinsteins per square meter per second) and low (80 microeinsteins per square meter per second) light. high-light leaves were thicker than low-light leaves and had greater development of the mesophyll. within a light level, high-nutrient leaves were thicker, but the proportions of leaf tissues did not change with nutrient level. maximum net co(2) exchange rate and ...198216662610
strawberry fruit protein with a novel indole-acyl modification.achenes and receptacle tissue of fragaria vesca, l. cultivar yellow wonder were shown to contain conjugated indole-3-acetic acid (iaa) that was not soluble in organic solvents and yielded iaa after strong alkaline hydrolysis, suggestive of iaa attached to plant proteins. this solvent insoluble conjugated iaa accounted for between 0.4 and 4 ng of iaa per gram fresh weight of tissue in both achenes and receptacles. to investigate this strawberry conjugate class further, a polyclonal antibody was p ...200616683161
the strawberry gene fagast affects plant growth through inhibition of cell elongation.the strawberry (fragaria x ananassa) fagast gene encodes a small protein with 12 cysteine residues conserved in the c-terminal region similar to a group of proteins identified in other species with diverse assigned functions such as cell division, elongation, or elongation arrest. this gene is expressed in the fruit receptacle, with two peaks during ripening at the white and the red-ripe stages, both coincident with an arrest in the growth pattern. expression is also high in the roots but confin ...200616804055
growth onset, senescence, and reproductive development of meadow species in mesocosms exposed to elevated o3 and co2.we studied the effects of elevated o3 (40-50 ppb) and co2 (+100 ppm) alone and in combination on the growth onset, relative chlorophyll meter values, and reproductive development of meadow species grown in ground-planted mesocosms using open-top chambers. the 3-year study was conducted in the summers of 2002-2004. elevated o3 decreased the early season coverage of plant communities and delayed the flowering of campanula rotundifolia and vicia cracca. the relative chlorophyll meter values of frag ...200716815612
correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from bulgarian herbs.the water phase antioxidant activity of extracts from 23 bulgarian medicinal plants was studied in relation to their polyphenol content in comparison with mate, black tea, honeybush and rooibos foreign species. antioxidant activity was measured by the abts (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) cation radical decolorization assay, and the total polyphenol content was assayed according to the folin-ciocalteu method. five bulgarian plant extracts exhibited higher antioxidant activ ...200616906640
small-scale heterogeneity in soil quality influences photosynthetic efficiency and habitat selection in a clonal plant.in clonal plants, internode connections allow translocation of photosynthates, water, nutrients and other substances among ramets. clonal plants form large systems that are likely to experience small-scale spatial heterogeneity. physiological and morphological responses of fragaria vesca to small-scale heterogeneity in soil quality were investigated, together with how such heterogeneity influences the placement of ramets. as a result of their own activities plants may modify the suitability of t ...200616987921
molecular interaction between methylobacterium extorquens and seedlings: growth promotion, methanol consumption, and localization of the methanol emission site.four methylobacterium extorquens strains were isolated from strawberry (fragaria x ananassa cv. elsanta) leaves, and one strain, called me4, was tested for its ability to promote the growth of various plant seedlings. seedling weight and shoot length of nicotiana tabacum, lycopersicon esculentum, sinapis alba, and fragaria vesca increased significantly in the presence of the pink-pigmented facultative methylotroph (ppfm), but the germination behaviour of seeds from six other plants was not affec ...200617043084
cytological studies on polyploids derived from tetraploid fragaria vesca and cultivated strawberries. 195117247349
active package for wild strawberry fruit (fragaria vesca l.).an antimicrobial active package has been developed to improve the safety and quality of wild strawberries, as well as extending their shelf life. the fruits were packed in equilibrium-modified atmosphere packaging (emap), and the effect on botrytis cinerea growth and on the quality parameters of the fruit by the addition of different amounts of 2-nonanone, an antifungal volatile compound naturally present in strawberries, was investigated during storage at 10 and 22 degrees c. the temperature of ...200717316018
characterization of polyphenol oxidase and peroxidase and influence on browning of cold stored strawberry fruit.polyphenol oxidase and peroxidase were extracted from two different varieties of strawberry fruit (fragaria x ananassa d, cv. 'elsanta' and fragaria vesca l, cv. 'madame moutot') and characterized using reliable spectrophotometric methods. in all cases, the enzymes followed michaelis-menten kinetics, showing different values of peroxidase kinetics parameters between the two cultivars: km = 50.68 +/- 2.42 mm ('elsanta') and 18.18 +/- 8.79 mm ('madame moutot') mm and vmax = 0.14 +/- 0.03 u/g ('els ...200717407312
wild vascular plants gathered for consumption in the polish countryside: a review.this paper is an ethnobotanical review of wild edible plants gathered for consumption from the end of the 18th century to the present day, within the present borders of poland.200717433114
first comprehensive contribution to medical ethnobotany of western pyrenees.an ethnobotanical and medical study was carried out in the navarre pyrenees, an area known both for its high biological diversity and its cultural significance. as well as the compilation of an ethnopharmacological catalogue, a quantitative ethnobotanical comparison has been carried out in relation to the outcomes from other studies about the pyrenees. a review of all drugs used in the area has also been carried out, through a study of the monographs published by the institutions and organizatio ...200717553138
traditional knowledge of wild edible plants used in the northwest of the iberian peninsula (spain and portugal): a comparative study.we compare traditional knowledge and use of wild edible plants in six rural regions of the northwest of the iberian peninsula as follows: campoo, picos de europa, piloña, sanabria and caurel in spain and parque natural de montesinho in portugal.200717555572
[an orientational examination of the effects of extracts from mixtures of herbal drugs on selected renal functions].the paper aimed to determine the effects of mixtures of selected medicinal plants on some physiological renal functions, i.e. excretion of urine and electrolytes and changes in the quantity of prostaglandins e2 (pge2) and kallikrein-kinins in rat blood plasma after water and salt load. the following medicinal plants were selected for the examination: downy birch (betula pubescens ehrh.), everlasting flower (helichrysum arenarium l. moench.), hawthorn (crataegus oxyacantha l.), woodland strawberr ...200717619305
effects of physical connection and genetic identity of neighbouring ramets on root-placement patterns in two clonal species.root-placement patterns were examined in the clonal species glechoma hederacea and fragaria vesca when grown with different types of neighbours. three different patterns were predicted as consequences of different types of interactions between roots: the avoidance pattern if root growth decreases in the presence of neighbouring roots; the intrusive pattern if root growth increases towards neighbouring roots; and the unresponsive pattern if root growth is unaffected by neighbouring roots. experim ...200717850258
partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (fragaria vesca).in addition to the role of the cell wall as a physical barrier against pathogens, some of its constituents, such as pectin-derived oligogalacturonides (oga), are essential components for elicitation of defence responses. to investigate how modifications of pectin alter defence responses, we expressed the fruit-specific fragaria x ananassa pectin methyl esterase fape1 in the wild strawberry fragaria vesca. pectin from transgenic ripe fruits differed from the wild-type with regard to the degree an ...200818088306
high diversity of arbuscular mycorrhizal fungi in a boreal herb-rich coniferous forest.* here, the diversity of arbuscular mycorrhizal (am) fungi was determined in a boreal herb-rich coniferous forest in relation to environmental variables. * root samples of five plant species (fragaria vesca, galeobdolon luteum, hepatica nobilis, oxalis acetosella and trifolium pratense) were analysed from stands differing in age and forest management intensity. * thirty-four glomeromycota taxa (small-subunit ribosomal rna gene (ssu rdna) sequence groups) were detected from 90 root samples (911 c ...200818537886
genetic diversity of pto-like serine/threonine kinase disease resistance genes in cultivated and wild strawberries.degenerate oligonucleotide primers, designed based on conserved regions of several serine-threonine kinases (stk) previously cloned in tomato and arabidopsis, were used to isolate stk candidates in wild and cultivated strawberries. seven distinct classes of stks were identified from three related wild species, i.e., fragaria vesca, fragaria chiloensis, and potentilla tucumanensis, and seven different fragaria x ananassa cultivars. alignment of the deduced amino acid sequences and the pto r prote ...200818618068
[contents of tannins and oxalic acid in the selected forest fruits depending on the harvest site].contents of anti-nutritional components (tannins and oxalic acid) were determined in samples of forest fruits: blueberry, raspberry and wild strawberry harvested in lublin region from areas considered as potentially not exposed to pollution (skierbieszów landscape park) and potentially polluted areas (cement factory rejowiec s.a.). study revealed that blueberry and raspberry fruits collected on potentially polluted area were characterized by higher tannins contents than those harvested on potent ...200818666621
sex ratio represents a unique context for selection on attractive traits: consequences for the evolution of sexual dimorphism.we explored the idea that sex ratio represents a unique context for selection on attractive traits by manipulating sex ratio and pollinator abundance in experimental populations of a gender-dimorphic wild strawberry fragaria virginiana. we found that increasing the frequency of functional males (the pollen-bearing morph) increased the frequency of pollen-collecting syrphid flies in the pollinator assemblage, decreased pollinator visitation to less preferred morph (females), and decreased the deg ...200118707294
genetic mapping of sex determination in a wild strawberry, fragaria virginiana, reveals earliest form of sex chromosome.the evolution of separate sexes (dioecy) from hermaphroditism is one of the major evolutionary transitions in plants, and this transition can be accompanied by the development of sex chromosomes. studies in species with intermediate sexual systems are providing unprecedented insight into the initial stages of sex chromosome evolution. here, we describe the genetic mechanism of sex determination in the octoploid, subdioecious wild strawberry, fragaria virginiana mill., based on a whole-genome sim ...200818797475
[content of selected metals in forest fruits depending on the harvest site].contents of selected metals (pb, cd, cu, zn, fe, mn) were determined in samples of forest fruits: blueberry, raspberry and wild strawberry harvested in lublin region from areas considered as potentially not exposed to pollution (skierbieszów landscape park) and potentially polluted areas (cement factory rejowiec s.a.). analyzed fruits originating from stands more exposed to pollution were characterized by higher lead (except from raspberry) as well as other metals contents than those from krasic ...200818807913
florivory increases selfing: an experimental study in the wild strawberry, fragaria virginiana.florivores are antagonists that damage flowers, and have direct negative effects on flowering and pollination of the attacked plants. while florivory has mainly been studied for its consequences on seed production or siring success, little is known about its impact on mating systems. damage to flowers can alter pollinator attraction to the plant and may therefore modify patterns of pollen transfer. however, the consequences of damage for mating systems can take two forms: a decrease in flower nu ...200919121112
cardiovascular effects in vitro of aqueous extract of wild strawberry (fragaria vesca, l.) leaves.in contrast to the strawberry fruits, strawberry leaves as a source of bioactive compounds with potentially beneficial biological effects have been largely overlooked. in this study we examined direct, dose-dependent effects of wild strawberry (fragaria vesca, l.) leaves aqueous extract, in two experimental models and animal species, the isolated guinea pig hearts and rat aortic rings. vasodilatory potential of the wild strawberry leaves extract was compared with vasodilatory activity of aqueous ...200919131227
assessment of anti-oxidant activity of plant extracts using microbial test systems.to evaluate the anti-oxidant properties of extracts from 20 medicinal herbs growing in western siberia using microbial test systems and different in vitro methods.200919187149
allopolyploidy in fragariinae (rosaceae): comparing four dna sequence regions, with comments on classification.potential events of allopolyploidy may be indicated by incongruences between separate phylogenies based on plastid and nuclear gene sequences. we sequenced two plastid regions and two nuclear ribosomal regions for 34 ingroup taxa in fragariinae (rosaceae), and six outgroup taxa. we found five well supported incongruences that might indicate allopolyploidy events. the incongruences involved aphanes arvensis, potentilla miyabei, potentilla cuneata, fragaria vesca/moschata, and the drymocallis clad ...200919268709
[agamospermy in outbred crosses of fragaria vesca l. (rosaceae)].the seed progeny obtained in european wood strawberry fragaria vesca l. (2n = 2x = 14) via outbred crosses proved to include matromorphic diploid plants, whose proportion varied from 10.0 to 21.0%. to allow their identification, the crosses were performed using recessive dihomozygotes (variety alexandria) as a maternal form and dominant dihomozygotes as a paternal form. generation of parthenogenetic plants in ourbred crosses with known genetic markers suggested pseudogamous agamospermy. the ques ...200919382689
search for a plant for phytoremediation--what can we learn from field and hydroponic studies?the main aim of the study was to evaluate the strategies for coping with arsenic toxicity developed by the mine species (calamagrostis arundinacea, fragaria vesca, stachys sylvatica, and epilobium parviflorum), and to compare results obtained from plants exposed to arsenic present in contaminated soil (2000-3500 mg/kg dw) and in hydroponic solution (2 microm and 12 microm arsenate). here we report basic differences in plant responses to arsenic depending on growth conditions (hydroponic/soil) wi ...200919733893
the development and characterisation of a bacterial artificial chromosome library for fragaria vesca.the cultivated strawberry fragaria xananassa is one of the most economically-important soft-fruit species. few structural genomic resources have been reported for fragaria and there exists an urgent need for the development of physical mapping resources for the genus. the first stage in the development of a physical map for fragaria is the construction and characterisation of a high molecular weight bacterial artificial chromosome (bac) library.200919772672
identification of flowering genes in strawberry, a perennial sd plant.we are studying the regulation of flowering in perennial plants by using diploid wild strawberry (fragaria vesca l.) as a model. wild strawberry is a facultative short-day plant with an obligatory short-day requirement at temperatures above 15 degrees c. at lower temperatures, however, flowering induction occurs irrespective of photoperiod. in addition to short-day genotypes, everbearing forms of wild strawberry are known. in 'baron solemacher' recessive alleles of an unknown repressor, seasonal ...200919785732
high variation in clonal vs. sexual reproduction in populations of the wild strawberry, fragaria virginiana (rosaceae).many plants reproduce both clonally and sexually, and the balance between the two modes of reproduction will vary among populations. clonal reproduction was characterized in three populations of the wild strawberry, fragaria virginiana, to determine the extent that reproductive mode varied locally between sites. the study sites were fragmented woodlands in cook county, illinois, usa.200919797422
an inbred line of the diploid strawberry fragaria vesca f. semperflorens for genomic and molecular genetic studies in the rosaceae.abstract:200919878589
[evaluation of anti-inflammatory activity of extracts from siberian plants].experimental investigations have shown that water-alcohol extracts from plants containing alkaloids (aconitum baikalense, aconitum septentrionale, delphinium elatum l., conium maculatum) and salicylic acid (filipendula ulmaria, salix viminalis, fragaria vesca, rubus idaeus) inhibited the development of main symptoms of inflammation, viz. exudation, pain, fever, to the same extent as non-steroidal anti-inflammatory agents. the substances studied in this work may be used to develop new efficient p ...200920017405
larval survival of fuller's rose weevil, naupactus cervinus, on common groundcover species in orchards of new zealand kiwifruit.fuller's rose weevil, naupactus cervinus (boheman) (curculionidae: entiminae), is an important quarantine pest of new zealand kiwifruit exported to asian markets. both adults and larvae are considered to be polyphagous. in this study, the survival of larval n. cervinus was estimated on common groundcover species of kiwifruit (actinidia spp.) in the bay of plenty, the main region in new zealand where kiwifruit is grown. the botanical composition of groundcover in commercial kiwifruit orchards, ch ...200820298112
snp discovery and genetic mapping of t-dna insertional mutants in fragaria vesca l.as part of a program to develop forward and reverse genetics platforms in the diploid strawberry [fragaria vesca l.; (2n = 2x = 14)] we have generated insertional mutant lines by t-dna mutagenesis using pcambia vectors. to characterize the t-dna insertion sites of a population of 108 unique single copy mutants, we utilized thermal asymmetric interlaced pcr (hitail-pcr) to amplify the flanking region surrounding either the left or right border of the t-dna. bioinformatics analysis of flanking seq ...201020349033
implementing reverse genetics in rosaceae: analysis of t-dna flanking sequences of insertional mutant lines in the diploid strawberry, fragaria vesca.reverse genetics is used for functional genomics research in model plants. to establish a model system for the systematic reverse genetics research in the rosaceae family, we analyzed genomic dna flanking the t-dna insertions in 191 transgenic plants of the diploid strawberry, fragaria vesca. one hundred and seventy-six t-dna flanking sequences were amplified from the right border (rb) and 37 from the left border (lb) by thermal asymmetric interlaced pcr. analysis of the t-dna nick positions rev ...201020444194
determination of lead, cadmium, and persistent organic pollutants in wild and orchard-farm-grown fruit in northeastern poland.the concentrations of the heavy metals lead (pb) and cadmium (cd) were determined in berries (blackberry, raspberry, bilberry, wild strawberry), and hazelnuts picked from plants in the wild as well as in fruit (blackberry, raspberry, blueberry) and hazelnuts picked from orchard-farmed plants in northeastern poland. the levels of seven congeners of polychlorinated biphenyls (pcb(7)), gamma isomer of hexachlorocyclohexane (gamma-hch), and sum of dichlorodiphenyl trichloroethane and its metabolites ...201020706949
generation and analysis of ests from strawberry (fragaria xananassa) fruits and evaluation of their utility in genetic and molecular studies.cultivated strawberry is a hybrid octoploid species (fragaria xananassa duchesne ex. rozier) whose fruit is highly appreciated due to its organoleptic properties and health benefits. despite recent studies on the control of its growth and ripening processes, information about the role played by different hormones on these processes remains elusive. further advancement of this knowledge is hampered by the limited sequence information on genes from this species, despite the abundant information av ...201020849591
comparative genetic mapping points to different sex chromosomes in sibling species of wild strawberry (fragaria).separate sexes have evolved repeatedly from hermaphroditic ancestors in flowering plants, and thus select taxa can provide unparalleled insight into the evolutionary dynamics of sex chromosomes that are thought to be shared by plants and animals alike. here we ask whether two octoploid sibling species of wild strawberry--one almost exclusively dioecious (males and females), fragaria chiloensis, and one subdioecious (males, females, and hermaphrodites), f. virginiana--share the same sex-determini ...201020923978
the genome of woodland strawberry (fragaria vesca).the woodland strawberry, fragaria vesca (2n = 2x = 14), is a versatile experimental plant system. this diminutive herbaceous perennial has a small genome (240 mb), is amenable to genetic transformation and shares substantial sequence identity with the cultivated strawberry (fragaria × ananassa) and other economically important rosaceous plants. here we report the draft f. vesca genome, which was sequenced to ×39 coverage using second-generation technology, assembled de novo and then anchored to ...201121186353
genome-wide snp identification by high-throughput sequencing and selective mapping allows sequence assembly positioning using a framework genetic linkage map.determining the position and order of contigs and scaffolds from a genome assembly within an organism's genome remains a technical challenge in a majority of sequencing projects. in order to exploit contemporary technologies for dna sequencing, we developed a strategy for whole genome single nucleotide polymorphism sequencing allowing the positioning of sequence contigs onto a linkage map using the bin mapping method.201021192788
demethylation of oligogalacturonides by fape1 in the fruits of the wild strawberry fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruit.ectopic expression of the strawberry (fragaria×ananassa) gene fape1 encoding pectin methyl esterase produced in the wild species fragaria vesca partially demethylated oligogalacturonides (ogas), which conferred partial resistance of ripe fruits to the fungus botrytis cinerea. analyses of metabolic and transcriptional changes in the receptacle of the transgenic fruits revealed channelling of metabolites to aspartate and aromatic amino acids as well as phenolics, flavanones, and sesquiterpenoids, ...201121273336
searching for gene flow from cultivated to wild strawberries in central europe.experimental crosses between the diploid woodland strawberry (fragaria vesca l.) and the octoploid garden strawberry (f. × ananassa duch.) can lead to the formation of viable hybrids. however, the extent of such hybrid formation under natural conditions is unknown, but is of fundamental interest and importance in the light of the potential future cultivation of transgenic strawberries. a hybrid survey was therefore conducted in the surroundings of ten farms in switzerland and southern germany, w ...201121307039
effect of fruit extract of fragaria vesca l. on experimentally induced inflammatory bowel disease in albino rats.ulcerative colitis and crohn's disease are chronic recurrent inflammatory bowel disease (ibd) of unknown origin. oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in ibd.201121455415
decaploidy in fragaria iturupensis (rosaceae).the strawberry genus, fragaria (rosaceae), has a base chromosome number of x = 7. cultivated strawberries (f. ×ananassa nothosubsp. ananassa) are octoploid (2n = 8x = 56) and first hybridized from f. chiloensis subsp. chiloensis forma chiloensis × f. virginiana subsp. virginiana. europe has no known native octoploid species, and only one asian octoploid species has been reported: f. iturupensis, from iturup island. our objective was to examine the chromosomes of f. iturupensis. ploidy levels of ...200921628226
an autotetraploid linkage map of rose (rosa hybrida) validated using the strawberry (fragaria vesca) genome sequence.polyploidy is a pivotal process in plant evolution as it increase gene redundancy and morphological intricacy but due to the complexity of polysomic inheritance we have only few genetic maps of autopolyploid organisms. a robust mapping framework is particularly important in polyploid crop species, rose included (2n = 4x = 28), where the objective is to study multiallelic interactions that control traits of value for plant breeding. from a cross between the garden, peach red and fragrant cultivar ...201121647382
are flower-visiting ants mutualists or antagonists? a study in a gynodioecious wild strawberry.ants are common flower visitors, but their effects on plant reproductive fitness have not often been assessed. flower-visiting ants were studied to determine whether they are antagonists or mutualists and whether they could influence floral or breeding system evolution in gynodioecious wild strawberry (fragaria virginiana). ant and flying pollinator (bees/flies) access to plants was manipulated, and visitation, fruit, and seed set were assessed. ants visited flowers of hermaphrodites more often ...200521652471
is relative pollen production or removal a good predictor of relative male fitness? an experimental exploration with a wild strawberry (fragaria virginiana, rosaceae).understanding plant reproduction requires knowledge of genetic contributions through pollen and seeds. since direct genetic assessments of fitness through pollen are often intractable, reproductive ecologists use components of male fitness such as pollen production and pollen removal as surrogates tot paternity. however, we know little of the strength of the relationship between these components and actual paternity. here, i report on a study undertaken to examine the relationship of pollen prod ...199821685001
pseudomonas fluorescens f113 mutant with enhanced competitive colonization ability and improved biocontrol activity against fungal root pathogens.motility is one of the most important traits for efficient rhizosphere colonization by pseudomonas fluorescens f113rif (f113). in this bacterium, motility is a polygenic trait that is repressed by at least three independent pathways, including the gac posttranscriptional system, the wsp chemotaxis-like pathway, and the sadb pathway. here we show that the kinb gene, which encodes a signal transduction protein that together with algb has been implicated in alginate production, participates in swim ...201121685161
metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway.wild strawberry (fragaria vesca) fruit contains several important phenylpropene aroma compounds such as eugenol, but cultivated varieties are mostly devoid of them. we have redirected the carbon flux in cultivated strawberry (fragaria×ananassa) fruit from anthocyanin pigment biosynthesis to the production of acetates of hydroxycinnamyl alcohols, which serve as the precursors of the phenylpropenes, by downregulating the strawberry chalcone synthase (chs) via rnai-mediated gene silencing and, alte ...201121689779
the tfl1 homologue ksn is a regulator of continuous flowering in rose and strawberry.flowering is a key event in plant life, and is finely tuned by environmental and endogenous signals to adapt to different environments. in horticulture, continuous flowering (cf) is a popular trait introduced in a wide range of cultivated varieties. it played an essential role in the tremendous success of modern roses and woodland strawberries in gardens. cf genotypes flower during all favourable seasons, whereas once-flowering (of) genotypes only flower in spring. here we show that in rose and ...201221895811
functional characterization of gynodioecy in fragaria vesca ssp. bracteata (rosaceae).gynodioecy is a phylogenetically widespread and important sexual system where females coexist with hermaphrodites. because dioecy can arise from gynodioecy, characterization of gynodioecy in close relatives of dioecious and sub-dioecious species can provide insight into this transition. thus, we sought to determine whether fragaria vesca ssp. bracteata, a close relative to f. chiloensis and f. virginiana, exhibits the functional and population genetic hallmarks of a gynodioecious species.201222052984
hepatoprotective activity of a phytotherapeutic formula on thioacetamide--induced liver fibrosis model.hepatic fibrosis is a widespread alteration in the liver that primarily consists of increased collagen deposition in the tissue. the aim of the present study was to evaluate the protective effects of poly-phytocompound eh-1501 containing small amounts of silymarin but also other potentially effective substances on thioacetamide (tta)-induced liver fibrosis and to elucidate the mechanisms underlying these protective effects in rats. forty rats were randomly divided into four groups. liver fibrosi ...201122069962
white-fruited duchesnea indica (rosaceae) is impaired in ans gene expression.duchesnea indica is a wild strawberry-like species that has red fruits. in a recent survey in the highlands of tucumán (argentina), a plant of d. indica with white fruits was discovered. the aim of this study was to investigate whether the white-fruited character was due to a phenotypic or genotypic change. the stability and heritability of the character and the expression of genes involved in anthocyanins synthesis were studied and compared with red-fruited genotypes. this study contributes to ...201122130272
flower and early fruit development in a diploid strawberry, fragaria vesca.the diploid woodland strawberry, fragaria vesca, is being recognized as a model for the more complex octoploid commercial strawberry, fragaria × ananassa. f. vesca exhibits a short seed to seed cycle, can be easily transformed by agrobacteria, and a draft genome sequence has been published. these features, together with its similar flower structure, potentially make f. vesca a good model for studying the flower development of other members of the rosaceae family, which contains many economically ...201222198460
computational identification of micrornas in strawberry expressed sequence tags and validation of their precise sequences by mir-race.micrornas (mirnas) are small, endogenously expressed, nonprotein-coding rnas that regulate gene expression at the post-transcriptional level in both animals and plants through repressing translation or inducing mrna degradation. a comprehensive strategy to identify new mirna homologs by mining the repository of available strawberry expressed sequence tags (ests) was developed. by adopting a range of filtering criteria, we identified 11 potential mirnas belonging to 5 mirna families from 47 890 f ...201222287696
strategies for transcriptome analysis in nonmodel plants.even with recent reductions in sequencing costs, most plants lack the genomic resources required for successful short-read transcriptome analyses as performed routinely in model species. several approaches for the analysis of short-read transcriptome data are reviewed for nonmodel species for which the genome of a close relative is used as the reference genome. two approaches using a data set from phytophthora-challenged rubus idaeus (red raspberry) are compared. over 70000000 86-nt illumina rea ...201222301897
chlorogenic acid, rutin and hyperoside content in fragaria vesca, f. viridis and f. moschata in lithuania.in lithuania, two species of the genus fragaria l. (rosaceae), f. vesca l. and f. viridis weston, occur naturally in the wild and two others, f. moschata weston and f. × ananassa duchesne ex rozier are found escaped from cultivation. the main objective of this study was to establish the variation pattern in the content of chlorogenic acid, rutin and hyperoside in leaves and fruits of the native lithuanian species. in this work, the chemical polymorphisms of different fragaria species were studie ...201322324400
clarifying the identity of the main ellagitannin in the fruit of the strawberry, fragaria vesca and fragaria ananassa duch.although the composition of strawberry fruit has been extensively studied, especially for the most abundant phenolic compounds, agrimoniin has never been univocally identified as one of the most abundant phenolic compounds in the fruit. in this study agrimoniin was isolated in the fruit of fragaria vesca and its structure characterized. furthermore, its presence was definitively established to be the main ellagitannin in both f. vesca and fragaria ananassa d. fruit. the presence of sanguiin h-6 ...201222339338
metabolite profiling reveals novel multi-level cold responses in the diploid model fragaria vesca (woodland strawberry).winter freezing damage is a crucial factor in overwintering crops such as the octoploid strawberry (fragaria × ananassa duch.) when grown in a perennial cultivation system. our study aimed at assessing metabolic processes and regulatory mechanisms in the close-related diploid model woodland strawberry (fragaria vescal.) during a 10-days cold acclimation experiment. based on gas chromatography/time-of-flight-mass spectrometry (gc/tof-ms) metabolite profiling of three f. vesca genotypes, clear dis ...201222370221
construction of black (rubus occidentalis) and red (r. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach.the genus rubus belongs to the rosaceae and is comprised of 600-800 species distributed world-wide. to date, genetic maps of the genus consist largely of non-transferable markers such as amplified fragment length polymorphisms. an f(1) population developed from a cross between an advanced breeding selection of rubus occidentalis (96395s1) and r. idaeus 'latham' was used to construct a new genetic map consisting of dna sequence-based markers. the genetic linkage maps presented here are constructe ...201222398438
physical mapping of a pollen modifier locus controlling self-incompatibility in apricot and synteny analysis within the rosaceae.s-locus products (s-rnase and f-box proteins) are essential for the gametophytic self-incompatibility (gsi) specific recognition in prunus. however, accumulated genetic evidence suggests that other s-locus unlinked factors are also required for gsi. for instance, gsi breakdown was associated with a pollen-part mutation unlinked to the s-locus in the apricot (prunus armeniaca l.) cv. 'canino'. fine-mapping of this mutated modifier gene (m-locus) and the synteny analysis of the m-locus within the ...201222481163
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