interactions between lactobacillus sakei and cnc (staphylococcus xylosus and kocuria varians) and their influence on proteolytic evaluate interactions between lactobacillus sakei and coagulase negative cocci (cnc) (staphylococcus xylosus and kocuria varians) and to investigate the influence of these interactions on their own proteolytic activity.201020875035
endophytic bacteria associated with growing shoot tips of banana (musa sp.) cv. grand naine and the affinity of endophytes to the host.a cultivation-based assessment of endophytic bacteria present in deep-seated shoot tips of banana suckers was made with a view to generate information on the associated organisms, potential endophytic contaminants in tissue-cultured bananas and to assess if the endophytes shared a beneficial relationship with the host. plating the tissue homogenate from the central core of suckers showed colony growth on nutrient agar from just 75% and 42% of the 12 stocks during may and november, respectively ( ...200919633807
inhibitory effect of oxalic acid on bacterial spoilage of raw chilled chicken.oxalic acid was evaluated as a treatment for reducing populations of naturally occurring microorganisms on raw chicken. raw chicken breasts were dipped in solutions of oxalic acid (0, 0.5, 1.0, 1.5, and 2.0%, wt/vol) for 10, 20, and 30 min, individually packed in oxygen-permeable polyethylene bags, and stored at 4 degrees c. total plate counts of aerobic bacteria and populations of pseudomonas spp. and enterobacteriaceae on breasts were determined before treatment and after storage for 1, 3, 7, ...200616924917
comparative bioremediation potential of four rhizospheric microbial species against lindane.four microbial species (kocuria rhizophila, microbacterium resistens, staphylococcus equorum and staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a lindane contaminated environment and acclimatized in lindane spiked media (5-100 μg ml⁻¹). the isolated species were inoculated with soil containing 5, 50 and 100 mg kg⁻¹ of lindane and incubated at room temperature. soil samples were collected periodically to evaluate the microbial ...201021044795
microbiological characteristics of "androlla", a spanish traditional pork sausage.counts of total aerobic mesophilic microflora, lactic acid bacteria, salt-tolerant microflora, enterobacteriaceae, enterococci, moulds and yeasts, and staphylococci, and some physico-chemical parameters (total solids, nacl and nitrate contents and ph and aw values) were determined in 20 units of "androlla", a traditional dry-fermented sausage made in the nw of spain. in general, high counts of all the investigated microbial groups were observed, with average values of 8.99 +/- 0.46 log cfu/g for ...200716943094
decolorization and detoxification of sulfonated azo dye methyl orange by kocuria rosea mtcc 1532.kocuria rosea (mtcc 1532) showed 100% decolorization of methyl orange (50 mg l(-1)) under static condition. the optimum ph and temperature for dye decolorization was 6.8 and 30 degrees c, respectively. the k. rosea (mtcc 1532) showed maximum decolorization of methyl orange when growth medium containing yeast extract as compared to other substrates. the culture exhibited significant ability to decolorize repeated additions of dye, with reduction in time up to 12 h at eighth dye aliquot addition. ...201019969416
volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus).chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ...200521132979
kocuria atrinae sp. nov., isolated from traditional korean fermented seafood.a novel actinobacterium, strain p30(t), was isolated from jeotgal, a traditional korean fermented seafood. cells were aerobic, gram-positive, non-motile and coccoid. optimal growth occurred at 30-37 degrees c, at ph 8-9 and in the presence of 0-2 % (w/v) nacl. based on 16s rrna gene sequence analysis, strain p30(t) was phylogenetically closely related to kocuria carniphila, kocuria gwangalliensis, kocuria rhizophila, kocuria marina, kocuria rosea and k. varians with levels of similarity of 98.6, ...201019661502
two cases of peritonitis caused by kocuria marina in patients undergoing continuous ambulatory peritoneal dialysis.kocuria spp. are members of the micrococcaceae family that are frequently found in the environment and on human skin. few human infections have been reported. we describe what appear to be the first two cases of kocuria marina peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.200919692561
water sensitivity, antimicrobial, and physicochemical analyses of edible films based on hpmc and/or chitosan.several properties of chitosan films associated or not with hydroxypropylmethylcellulose polymer (hpmc) and hpmc films incorporating or not nisin and/or milk fat were studied. nisin addition at a level of 250 microg ml-1 and likewise chitosan at 1% (w/v) concentration were efficient for total inhibiting aspergillus niger and kocuria rhizophila food deterioration microorganisms. hpmc and chitosan films were transparent, whereas nisin and/or fat incorporation induced a 2-fold lightness parameter i ...200717263462
antibiotic resistance in lactic acid bacteria and micrococcaceae/staphylococcaceae isolates from artisanal raw milk cheeses, and potential implications on cheese making.antibiotic susceptibility against 19 antimicrobial agents was evaluated in isolates of the genera lactococcus (46 isolates), leuconostoc (22), lactobacillus (19), staphylococcus (8), enterococcus (7), and microccoccus/kocuria (5) obtained from the predominant microflora of nonrecent and recent types of artisanal raw cow's milk cheeses. beta-lactams showed broad activity against all genera, although leuconostocs and lactobacilli were highly resistant to oxacillin (80% to 95.5%). resistance to ami ...200919723213
catalase and superoxide dismutase double staining zymogram technique for deinococcus and kocuria species exposed to multiple stresses.superoxide dismutase (sod) and catalase expression is associated with oxidative stress. existing techniques for the individual staining of sod and catalase have been described in the past. the objective of this study was to achieve a simple and rapid technique for the double staining of bacterial sod and catalase on the same polyacrylamide gel. sod detection was carried out using nitro-blue tetrazolium (nbt) dye reduction followed by ferricyanide precipitation for negative staining of the catala ...200919810043
rice seeds as sources of endophytic bacteria.endophytic bacteria are considered to originate from the external environment. to examine the hypothesis that rice (oryza sativa, cultivar kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of the various bacterial strains isolated, the closest relatives, identified by 16s rrna gene sequencing, were: bacillus firmus, b. f ...200921566368
kocuria sm1 controls vibriosis in rainbow trout (oncorhynchus mykiss, walbaum).to develop probiotics for the control of vibriosis caused by vibrio anguillarum and vibrio ordalii in finfish.201019929950
genome sequence of the fleming strain of micrococcus luteus, a simple free-living actinobacterium.micrococcus luteus (nctc2665, "fleming strain") has one of the smallest genomes of free-living actinobacteria sequenced to date, comprising a single circular chromosome of 2,501,097 bp (g+c content, 73%) predicted to encode 2,403 proteins. the genome shows extensive synteny with that of the closely related organism, kocuria rhizophila, from which it was taxonomically separated relatively recently. despite its small size, the genome harbors 73 insertion sequence (is) elements, almost all of which ...201019948807
genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere.the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ...201121686169
kocuria koreensis sp. nov., isolated from fermented seafood.a gram-positive, aerobic, non-motile and coccoid actinobacterium, designated p31(t), was isolated from a traditional, fermented seafood. the strain was catalase-positive and oxidase-negative. cells grew in the presence of 0-15.0 % (w/v) nacl, and at ph 5-10 and 15-37 degrees c. major cellular fatty acids were anteiso-c(15 : 0), anteiso-c(17 : 0) and iso-c(16 : 0). strain p31(t) contained mk-7 as the predominant menaquinone. the dna g+c content of the genomic dna of strain p31(t) was 65.2 mol%. a ...201019648328
characterization of micrococcaceae isolated from iberian dry-cured sausages.the populations of micrococcaceae in different types of iberian dry-cured sausages from central-west spain were characterized and their technological and antimicrobial properties determined in order to evaluate their suitability as starter cultures in dry-cured sausage manufacture. of a total of four hundred strains isolated from two manufacturers, one hundred and sixty-six were selected to evaluate nitrate reductase, proteolytic, lipolytic, and antimicrobial activities, and growth at different ...200722064035
antimicrobial efficiency of titanium dioxide-coated surfaces.development and evaluation of an antimicrobially active titanium dioxide coating.201019886892
genes involved in long-chain alkene biosynthesis in micrococcus luteus.aliphatic hydrocarbons are highly appealing targets for advanced cellulosic biofuels, as they are already predominant components of petroleum-based gasoline and diesel fuels. we have studied alkene biosynthesis in micrococcus luteus atcc 4698, a close relative of sarcina lutea (now kocuria rhizophila), which 4 decades ago was reported to biosynthesize iso- and anteiso-branched, long-chain alkenes. the underlying biochemistry and genetics of alkene biosynthesis were not elucidated in those studie ...201020038703
kocuria salsicia sp. nov., isolated from salt-fermented seafood.strain 104(t) was isolated from a traditional salt-fermented seafood in korea. it was a gram-positive, non-motile, coccus-shaped bacterium. it formed lemon-yellow, opaque colonies that were circular with entire margins. optimal growth occurred at 30-37 °c, ph 7-8 and in the presence of 0-2 % (w/v) nacl. phylogenetic analysis of 16s rrna gene sequences from strain 104(t) and reference species of the genus kocuria indicated that strain 104(t) formed an independent line. the g+c content of the chro ...201120228215
Two-phase olive mill waste composting: community dynamics and functional role of the resident microbiota.In this study, physico-chemical modifications and community dynamics and functional role of the resident microbiota during composting of humid husk from a two-phase extraction system (TPOMW) were investigated. High mineralization and humification of carbon, low loss of nitrogen and complete degradation of polyphenols led to the waste biotransformation into a high-quality compost. Viable cell counts and denaturing gradient gel electrophoresis (DGGE) profiling of the 16S rRNA genes showed that the ...201121996482
metabolism of nitrate in fermented meats: the characteristic feature of a specific group of fermented foods.within the universe of food fermentation processes the multi-purpose use of nitrate and/or nitrite is a unique characteristic of meat fermentations. these curing agents play a decisive role in obtaining the specific sensory properties, stability and hygienic safety of products such as fermented sausages, ham and, more recently, emulsion type of sausages. the use of nitrate is the traditional method in curing processes and requires its reduction to reactive nitrite. thus, nitrate reduction is the ...201222202868
study on the removal of indoor vocs using biotechnology.with the improvement of living standard and civil building innovation, large numbers of building materials and decoration are being used. the emission of gaseous organic pollutants such as formaldehyde, benzene, toluene and xylene from these materials may trigger immediate or persistent potential harm to human health. in this study, with the combination of bake-out exhaust, biological treatment was applied to deal with volatile organic compounds (vocs). four bacterial strains were isolated from ...201020598440
behaviour of the constitutive biota of two types of spanish dry-sausages ripened in a pilot-scale chamber.the behaviour of the constitutive biota in eighty four samples belonging to two different types of spanish dry-cured sausages during the ripening process in a pilot-scale chamber was investigated. samples were analyzed in three stages during production: fresh product, first drying stage and finished product. lactic acid bacteria (lab) and coagulase-negative cocci (cnc) were identified by the api system. in general, evolution of lab and cnc during the ripening process of spanish dry-cured sausage ...200622062067
analysis of biogenic amines in northern and southern european sausages and role of flora in amine production.the biogenic amine contents, microbial counts and flora producing amines were investigated in four types of fermented sausages. southern type european sausages (italian and belgian) showed higher tyramine and phenylethylamine values than northern type ones (norwegian and belgian). the spontaneous non-starter lactic acid bacteria could be responsible for the production of these amines in the italian products, and the cocci gram positive in the belgian south ones. the norwegian sausages showed the ...200222064002
diversity of microorganisms in the environment and dry fermented sausages of small traditional french processing units.naturally fermented sausages produced in nine traditional french processing units and their environmental surfaces were characterised by microbial and physico-chemical analyses. salmonella and staphylococcus aureus were not detected in the environment whereas listeria monocytogenes was detected in four samples. staphylococcus/kocuria, lactic acid bacteria (lab), enterobacteriaceae, pseudomonas, yeasts/moulds and enterococci contaminated the surfaces of two processing units, indicating insufficie ...200722064197
identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ...201222074685
isolation and technological properties of coagulase negative staphylococci from fermented sausages of southern italy.the aims of this study were to characterize the population of micrococcaceae in different types of fermented sausages of southern italy and to determine the technological properties of staphylococcus strains in order to evaluate the suitability of selected strains as starter cultures in the processing of dry fermented pork sausages. ninety-six strains were studied to evaluate nitrate reductase, proteolytic, lipolytic and antioxidant activities as well as growth ability at different temperatures, ...200422061128
comparative analysis of micrococcus luteus isolates from blood cultures of patients with pulmonary hypertension receiving epoprostenol continuous infusion.during the period 2002-2008, at the national cardiovascular center, osaka, 28 micrococcus luteus isolates and one kocuria spp. isolate were obtained from blood cultures of pulmonary hypertension (ph) patients who were receiving continuous infusion therapy with epoprostenol. pulsed-field gel electrophoresis patterns of the isolates were unrelated, suggesting that the infections had multiple origins. the preparation of epoprostenol solution by patients themselves was thought to be a risk factor.200920012737
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
black tongue associated with kocuria (micrococcus) kristinae bacteremia in a 4-month-old infant. 201121935583
isolation and characterization of bacteria resistant to metallic copper surfaces.metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including escherichia coli, staphylococcus aureus, and clostridium difficile, are inactivated within minutes or a few hours of exposure. in this study, we show that bacteria isolated from copper alloy coins ...201020048058
effects of bovine milk lactoperoxidase system on some bacteria.bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ...201020198919
catheter-related bacteraemia and infective endocarditis caused by kocuria species.we describe five patients with positive blood culture for kocuria species. three patients had catheter-related bacteraemia and one had infective endocarditis caused by kocuria kristinae, and one had a k. marina isolate, which was considered to be a contaminant. identification of the isolates was further confirmed by 16s rrna gene sequence analysis. in conclusion, kocuria species are an unusual cause of infection in immunocompromised patients. accurate identification with molecular methods is imp ...201120218989
Arsenic accumulating and transforming bacteria isolated from contaminated soil for potential use in bioremediation.Arsenic (As) is a metalloid and considered harmful due to its toxic and carcinogenic effects. Removal of arsenic is of great importance for human welfare. The main objective of this study was to isolate arsenic-resistant bacteria that are capable of removing arsenic from the environment. Soil samples were collected from an arsenic-affected area of West Bengal, India and 10 different bacterial strains were isolated. The minimum inhibitory concentration (MIC) values of the isolates varied widely i ...201122175878
development of protection in rainbow trout (oncorhynchus mykiss, walbaum) to vibrio anguillarum following use of the probiotic kocuria sm1.probiotic kocuria sm1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (oncorhynchus mykiss) fingerlings as dietary supplement at approximately 10(8) cells g(-1) for two weeks. the fish were challenged with vibrio anguillarum and innate immunity determined over the following 5 weeks. the probiotic-fed fish survived (mortalities = 10-28%; p < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of ko ...201020371293
degradation of pentachlorophenol by kocuria sp. cl2 isolated from secondary sludge of pulp and paper mill.a pentachlorophenol (pcp) degrading bacterium was isolated and characterized from sludge of pulp and paper mill. this isolate used pcp as its sole source of carbon and energy and was capable of degrading this compound, as indicated by stoichiometric release of chloride and biomass formation. based on morphology, biochemical tests, and 16s rrna gene sequence analysis this strain was identified as kocuria sp. cl2. high performance liquid chromatography (hplc) analysis revealed that this strain was ...201120532592
salt-dependent thermo-reversible α-amylase: cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, kocuria varians.a moderately halophilic bacterium, kocuria varians, was found to produce active α-amylase (k. varians α-amylase (kva)). we have observed at least six different forms of α-amylase secreted by this bacterium into the culture medium. characterization of these kva forms and cloning of the corresponding gene revealed that kva comprises pre-pro-precursor form of α-amylase catalytic domain followed by the tandem repeats, which show high similarity to each other and to the starch binding domain (sbd) of ...201020871989
drug sensitivity and clinical impact of members of the genus kocuria.organisms in the genus kocuria are gram-positive, coagulase-negative, coccoid actinobacteria belonging to the family micrococcaceae, suborder micrococcineae, order actinomycetales. sporadic reports in the literature have dealt with infections by kocuria species, mostly in compromised hosts with serious underlying conditions. nonetheless, the number of infectious processes caused by such bacteria may be higher than currently believed, given that misidentification by phenotypic assays has presumab ...201020884772
inhibitory effects of lactic acid and lauricidin on spoilage organisms of chicken breast during storage at chilled temperature.different concentrations of lauricidin (lu, containing 1% lactic acid) and lactic acid alone (la) were evaluated for their effectiveness in reducing naturally occurring microflora of raw chicken breasts. chicken breasts were dipped in 0 (control), 0.5, 1.0, 1.5, and 2.0% solutions of lu (w/v) or la (v/v) for 10, 20, and 30 min and stored at 4°c for 14 d. total plate counts (tpc) and populations of pseudomonas spp. and enterobacteriaceae were determined before and after dipping and after storing ...201020947197
central venous catheter-related bacteremia caused by kocuria kristinae: case report and review of the literature.kocuria species are unusual human pathogens isolated most commonly from immunocompromised hosts, such as transplant recipients and cancer patients undergoing chemotherapy, or from patients with chronic medical conditions. a case of catheter-related bacteremia with pulmonary septic emboli in a pregnant adult female without chronic medical conditions is described. a review of other reported kocuria infections is provided.201121864336
analysis of the cultivable bacterial community in jeotgal, a korean salted and fermented seafood, and identification of its dominant bacteria.jeotgal or jeot, a traditional korean salted and fermented food, is made by adding 20-30% (w/w) salt to various types of seafood. to develop a more complete overview of the bacterial community present in jeotgal, 610 pure colonies were isolated from myeolchi-jeot and saeu-jeot, the most commonly consumed varieties of jeotgal, which are made with anchovy (engraulis japonicas) and tiny shrimp (acetes japonicas), respectively. the bacterial isolates were identified by 16s rdna sequence analysis. a ...201021056781
subcellular components of probiotics kocuria sm1 and rhodococcus sm2 induce protective immunity in rainbow trout (oncorhynchus mykiss, walbaum) against vibrio anguillarum.the efficacy of cellular components of probiotics kocuria sm1 and rhodococcus sm2 to protect rainbow trout (oncorhynchus mykiss, walbaum) against vibriosis was assessed. groups of fish (average weight = 10-15 g) were immunized intraperitoneally (i.p.) with 0.1 ml of subcellular materials, i.e., 0.2 ± 0.05 mg protein per fish, comprising extracellular proteins (ecps), cell wall proteins (cwps) and whole cell proteins (wcps) of sm1 and sm2, respectively, or with 0.1 ml of phosphate-buffered saline ...201021078398
isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi.soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ...201121219415
description of a novel actinobacterium kocuria assamensis sp. nov., isolated from a water sample collected from the river brahmaputra, assam, india.a gram-positive, pale yellow pigmented actinobacterium, strain s9-65(t) was isolated from a water sample collected from the river brahmaputra, assam, india and subjected to a polyphasic taxonomic study. the physiological and biochemical properties, major fatty acids (anteiso-c15:0 and anteiso-c17:0), estimated dna g+c content (69.2 mol %) and 16s rrna gene sequence analysis showed that strain s9-65(t) belonged to the genus kocuria. strain s9-65(t) exhibited highest 16s rrna gene sequence similar ...201121234681
kocuria kristinae: an unusual cause of acute peritoneal dialysis-related infection. 201121282394
an unusual organism for capd-related peritonitis: kocuria kristinae. 201121282396
the microbiota of an unpolluted calcareous soil faces up chlorophenols: evidences of resistant strains with potential for highlight the effects of a variety of chlorophenols (cp) in relation to the response of an indigenous bacterial community, an agricultural mediterranean calcareous soil has been studied in microcosms incubated under controlled laboratory conditions. soil samples were artificially polluted with 2-monochlorophenol (mcp), 2,4,6-trichlorophenol (tcp) and pentachlorophenol (pcp), at concentrations ranging from 0.1 up to 5000 mg kg(-1). both activity and composition of the microbial community were ...201121295817
detection, selective isolation and characterisation of dactylosporangium strains from diverse environmental samples.a culture-independent, nested pcr procedure based on genus-specific oligonucleotide primers detected the presence of members of the genus dactylosporangium in 14 out of 21 diverse environmental samples. clones generated from the 14 positive environmental samples formed distinct phyletic lines in the dactylosporangial 16s rrna gene tree. presumptive dactylosporangiae were isolated from 7 of these samples using a medium designed to be selective for members of the genus dactylosporangium, namely st ...201121596505
kocuria sediminis sp. nov., isolated from a marine sediment sample.a gram-positive, pinkish-orange pigmented, coccoid strain, fcs-11(t) was isolated from a marine sediment sample taken from kochi fort area, kerala, india and subjected to polyphasic taxonomic study. the 16s rrna gene sequence of the strain was determined and the results of 16s rrna gene sequence analysis showed that the strain fcs-11(t) should be assigned to the genus kocuria. the chemotaxonomic data supported this taxonomic placement i.e. menaquinones mk-7(h(2)), mk-8(h(2)) and mk-9(h(2)); majo ...201122012251
draft genome sequence of kocuria rhizophila p7-4.we report the draft genome sequence of kocuria rhizophila p7-4, which was isolated from the intestine of siganus doliatus caught in the pacific ocean. the 2.83-mb genome sequence consists of 75 large contigs (>100 bp in size) and contains 2,462 predicted protein-coding genes.201121685281
halophilic characterization of starch-binding domain from kocuria varians α-amylase.the tandem starch-binding domains (kvsbd) located at carboxy-terminal region of halophilic α-amylase from moderate halophile, kocuria varians, were expressed in e. coli with amino-terminal hexa-his-tag and purified to homogeneity. the recombinant kvsbd showed binding activity to raw starch granules at low to high salt concentrations. the binding activity of kvsbd to starch was fully reversible after heat-treatment at 85°c. circular dichroism and thermal scanning experiments indicated that kvsbd ...201222020156
isolation of biosurfactant producers, optimization and properties of biosurfactant produced by acinetobacter sp. from petroleum contaminated soil.aims:  to screen and identify biosurfactant producers from petroleum contaminated soil; to use response surface methodology (rsm) for medium optimization to enhance biosurfactant production; to study the properties of the newly obtained biosurfactant toward ph, temperature and salinity. methods and results:  we successfully isolated three biosurfactant producers from petroleum contaminated soil and identified them through 16s rrna sequence analysis, which exhibit the highest similarities to acin ...201222268814
gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities.dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ...201222234511
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