Publications

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development of colloidal gold-based immunochromatographic assay for rapid detection of mycoplasma suis in porcine plasma.a one-step immunochromatographic assay using gold nanoparticles coated with polyclonal antibody (pab) against mycoplasma suis (m. suis) was developed in this study for the detection of m. suis in porcine plasma. the colloidal gold was prepared by the reduction of gold salt with sodium citrate coupled with pab against m. suis. the pab was produced by immunizing the balb/c mice with recombinant msg1 (rmsg1) protein from m. suis expressed in escherichia coli. the optimal concentrations of the captu ...201424434494
occurrence and molecular characterization of bartonella spp. and hemoplasmas in neotropical primates from brazilian amazon.little is known about the prevalence and genetic diversity of bartonella spp. and hemoplasmas in nonhuman primates (nhp). the present study aimed to investigate the occurrence of and assess the phylogenetic position of bartonella spp. and hemoplasma species infecting neotropical nhp from brazilian amazon. from 2009 to 2013, a total of 98 blood samples from nhp belonging to the family cebidae were collected in the island of são luís, state of maranhão, of which 87 nhp were from wild animal screen ...201526577193
identification of mycoplasma suis msg1 interaction proteins on porcine erythrocytes.adhesion protein msg1 mediating adherence to porcine erythrocytes in mycoplasma suis (m. suis) invasion has been identified previously. in order to determine the host membrane proteins that interact with msg1, recombinant his-tagged msg1 (rmsg1) was used to screen for interacting proteins in the protein extracts of porcine erythrocyte membrane. potential rmsg1-interacting proteins were initially identified as band 3 and β-actin with molecular weight of 46 and 45 kda, respectively. immune fluores ...201525344885
high occurrence of mycoplasma suis infection in swine herds from non-technified farms in mossoró, state of rio grande do norte, northeastern brazil.mycoplasma suis, the etiological agent of swine hemoplasmosis, has been neglected in swine herds around the world. swine hemoplasmosis is frequently associated with hemolytic anemia, disgalacty, infertility and immunosuppression, and it results in significant economic losses. this study investigates the occurrence of m. suis in non-technified swine herds in the northeastern region of brazil using quantitative pcr (qpcr) based on the 16s rrna gene. between march and august 2013, blood samples fro ...201627982298
updating the proteome of the uncultivable hemotrophic mycoplasma suis in experimentally infected pigs.mycoplasma suis belongs to the hemotrophic mycoplasmas that are associated with acute and chronic anemia in a wide range of livestock and wild animals. the inability to culture m. suis in vitro has hindered its characterization at the molecular level. since the publication of m. suis genome sequences in 2011 only one proteome study has been published. aim of the presented study was to significantly extend the proteome coverage of m. suis strain ki_3806 during acute infection by applying three di ...201626678042
detection of mycoplasma haemocanis, mycoplasma haematoparvum, mycoplasma suis and other vector-borne pathogens in dogs from córdoba and santa fé, argentina.in argentina, only very few reports are available for canine tick-borne diseases where most are related to parasitic diseases. the objective of this survey was to investigate the prevalence of tick-borne pathogens in 70 dogs from santa fé and córdoba, argentina.201627978844
identification of a novel hemoplasma species from pigs in zhejiang province, china.hemoplasmas belong to mycoplasmataceae (mollicutes: mycoplasmatales) and are able to infect a broad range of mammalian species. we investigated prevalence of hemotropic mycoplasma species in pig farms in the region of zhejiang by a pcr scheme using universal primers targeting 16s rrna and rnase p rna gene (rnpb). representative positive samples from different farms were selected for sequencing of 16s rrna and the 219bp rnpb gene fragments for phylogenetic analysis. sequencing analysis of pcr pro ...201728381682
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