| insights into the gene expression profile of uncultivable hemotrophic mycoplasma suis during acute infection, obtained using proteome analysis. | hemotrophic mycoplasmas, bacteria without cell walls whose niche is the erythrocytes of their hosts, have never been cultivated in vitro. therefore, knowledge of their pathogenesis is fundamental. mycoplasma suis infects pigs, causing either acute fatal hemolytic anemia or chronic low-grade anemia, growth retardation, and immune suppression. recently, the complete genomes of two hemotrophic mycoplasma species, m. suis and m. haemofelis, were sequenced, offering new strategies for the analysis of ... | 2012 | 22267506 |
| updating the proteome of the uncultivable hemotrophic mycoplasma suis in experimentally infected pigs. | mycoplasma suis belongs to the hemotrophic mycoplasmas that are associated with acute and chronic anemia in a wide range of livestock and wild animals. the inability to culture m. suis in vitro has hindered its characterization at the molecular level. since the publication of m. suis genome sequences in 2011 only one proteome study has been published. aim of the presented study was to significantly extend the proteome coverage of m. suis strain ki_3806 during acute infection by applying three di ... | 2016 | 26678042 |
| identification of a novel hemoplasma species from pigs in zhejiang province, china. | hemoplasmas belong to mycoplasmataceae (mollicutes: mycoplasmatales) and are able to infect a broad range of mammalian species. we investigated prevalence of hemotropic mycoplasma species in pig farms in the region of zhejiang by a pcr scheme using universal primers targeting 16s rrna and rnase p rna gene (rnpb). representative positive samples from different farms were selected for sequencing of 16s rrna and the 219bp rnpb gene fragments for phylogenetic analysis. sequencing analysis of pcr pro ... | 2017 | 28381682 |
| [porcine eperythrozoonosis: from eperythrozoon suis to mycoplasma suis]. | mycoplasma suis (formerly known as eperythrozoon suis ) is the most prevalent agent causing haemolytic anaemia in swine. the disease is also known as porcine eperythrozoonosis. m.suis is a small, pleomorphic bacteria parasitizing porcine erythrocytes. to date, no in vitro cultivation system for m.suis has been established and, therefore, our knowledge about the characteristics of m.suis and the pathogenesis of porcine eperythrozoonosis is rather limited. m.suis can cause acute disease, ... | 2011 | 22138829 |
| use of msg1 protein in a novel blocking elisa for the detection of mycoplasma suis infection. | a blocking elisa based on the monoclonal antibody (mab) 1a7 was developed to detect antibodies against mycoplasma suis. the mab was produced by immunising balb/c mice with recombinant msg1 protein (rmsg1) from m. suis expressed in e. coli. following identification by western blotting, the mab was purified and labelled with horseradish peroxidase. the parameters of the elisa were optimised, and the cut-off value determined as 36.35% by analysing the percentage inhibition of m. suis negative serum ... | 2012 | 22285443 |
| genome sequence of mycoplasma parvum (formerly eperythrozoon parvum), a diminutive hemoplasma of the pig. | we report the complete genome sequence of mycoplasma parvum strain indiana. its circular chromosome is 564,395 bp, which is smaller than that of mycoplasma genitalium, which was previously considered the smallest member of the mollicutes. comparative analyses of the genomes of m. parvum and mycoplasma suis will provide novel insights into the molecular basis of their virulence. | 2013 | 24285648 |
| occurrence and molecular characterization of bartonella spp. and hemoplasmas in neotropical primates from brazilian amazon. | little is known about the prevalence and genetic diversity of bartonella spp. and hemoplasmas in nonhuman primates (nhp). the present study aimed to investigate the occurrence of and assess the phylogenetic position of bartonella spp. and hemoplasma species infecting neotropical nhp from brazilian amazon. from 2009 to 2013, a total of 98 blood samples from nhp belonging to the family cebidae were collected in the island of são luís, state of maranhão, of which 87 nhp were from wild animal screen ... | 2015 | 26577193 |