Publications

preparation and characterization of chinese yam polysaccharide plga nanoparticles and their immunological activity.	this paper first provides that chinese yam polysaccharide (cyp) is encapsulated by plga using a double emulsion solvent evaporation method and aims to screen the optimal preparation of cyp-plga nanoparticles (cypp) using response surface methodology (rsm). the volume ratio of the internal water phase to the organic phase (w1:o), the volume ratio of the primary emulsion to the external water phase (pe:w2) and the concentration of poloxamer 188 (f68) are deemed key variables for the encapsulation ...	2016	27374200
antioxidant and antitumor activities of the extracts from chinese yam (dioscorea opposite thunb.) flesh and peel and the effective compounds.	the aims of this study are to investigate the antioxidant and antitumor activities of the water and ethanol extracts isolated from chinese yam (dioscorea opposite thunb.) flesh (cyf) and peel (cyp) and the effective compounds. it was found that all peel portions have a better effect on reactive oxygen (ros) scavenging assay than meat portions, especially for the water extract of chinese yam peel (cyp-w). its ic50 values for hydroxyl radical (oh•) scavenging assay (744.25 ± 3.46 μg/ml) and for 1, ...	2016	27122252
high-throughput determination of multi-mycotoxins in chinese yam and related products by ultra fast liquid chromatography coupled with tandem mass spectrometry after one-step extraction.	a simple, accurate and sensitive ultra fast liquid chromatography coupled with tandem mass spectrometry (uflc-ms/ms) method was developed for high-throughput determination of aflatoxins (afb1, afb2, afg1 and afg2), ochratoxin a (ota), fumonisins (fb1 and fb2) and zearalenone (zea) in chinese yam, yam flours and yam-derived products. mycotoxins were extracted from the samples with methanol-water-formic acid (79:20:1, v/v/v) and no further cleanup step before analysis. after optimization of some c ...	2016	27085799
identification and validation of reference genes for qrt-pcr studies of gene expression in dioscorea opposita.	quantitative real-time polymerase chain reaction (qrt-pcr) is one of the most common methods for gene expression studies. data normalization based on reference genes is essential for obtaining reliable results for qrt-pcr assays. this study evaluated potential reference genes of chinese yam (dioscorea opposita thunb.), which is an important tuber crop and medicinal plant in east asia. the expression of ten candidate reference genes across 20 samples from different organs and development stages w ...	2016	27314014
complete genome sequence of chinese yam necrotic mosaic virus from dioscorea opposita in the republic of korea.	the complete genome sequence of chinese yam necrotic mosaic virus (chynmv) consisting of 8,213 nucleotides containing one open reading frame was determined by the transcriptome data generated from discorea opposita this is the first report of the complete nucleotide sequence of chynmv from dioscorea opposita in the republic of korea.	2016	27492000
chemical components and emulsification properties of mucilage from dioscorea opposita thunb.	the properties of mucilage obtained from dioscorea opposita, generated during industrial manufacturing were investigated in this study. characteristics such as monosaccharide content, amino acid content, molecular weight, and structural features were measured, whereas morphology was observed using a scanning/transmission electron microscope. additionally, emulsification properties at different concentrations (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) and under acidic and basic ph (5.0 and 9.0) condition ...	2017	28317729
rheological properties of polysaccharides from dioscorea opposita thunb.	this study investigated the chemical components and rheological properties of polysaccharides from dioscorea opposita thunb. graded alcohol precipitation was used to extract dioscorea opposita polysaccharide samples (s1, s2, s3 and s4). the monosaccharides, amino acid content and molecular weight of each sample were measured and compared. the rheological properties of the polysaccharide samples at different concentrations, temperatures and ph values were studied. the rheological properties of s1 ...	2017	28274459
emulsification properties of polysaccharides from dioscorea opposita thunb.	this study investigated the emulsification properties of polysaccharides from dioscorea opposita thunb. graded alcohol precipitation was used to extract dioscorea opposita polysaccharides fractions (4 samples) in different ranges of molecular weight. sample 3 contained more glucose and protein (80.13% and 0.34%, respectively), and molecular weight was approximately 34,790da, distributing narrowly. the droplet sizes and stabilities of emulsions made of gum arabic (ga) and polysaccharide samples a ...	2017	27979295
oral administration with diosgenin enhances the induction of intestinal t helper 1-like regulatory t cells in a murine model of food allergy.	although the development of t helper (th)1-like regulatory t (treg) cells under th1 inflammatory conditions has been reported, the role of th1-like treg cells in th2 allergic responses remains mostly unclear. we previously demonstrated that diosgenin, the major sapogenin contained in the chinese yam, attenuated food allergy and augmented th1 and treg immune responses. in this study, we hypothesized that diosgenin may enhance the induction of th1-like treg cells in the gut of mice with food aller ...	2017	27886644
development of repaglinide microspheres using novel acetylated starches of bitter and chinese yams as polymers.	tropical starches from dioscorea dumetorum (bitter) and dioscorea oppositifolia (chinese) yams were acetylated with acetic anhydride in pyridine medium and utilized as polymers for the delivery of repaglinide in microsphere formulations in comparison to ethyl cellulose. acetylated starches of bitter and chinese yams with degrees of substitution of 2.56 and 2.70 respectively were obtained. acetylation was confirmed by ftir, (1)h nmr spectroscopy. a 3(2) factorial experimental design was performed ...	2017	27769931
effects of planting and processing modes on the degradation of dithianon and pyraclostrobin in chinese yam (dioscorea spp.).	the yam (dioscorea spp.) is widely cultivated in china. the degradation of dithianon and pyraclostrobin in yams with different planting and processing treatments was investigated in this article. an analytical method for two pesticides in yam and yam plant was developed, and recoveries were between 77% and 93%, with relative standard deviations from 0.8% to 7.4%, respectively. on the basis of this method, half-lives for plants grown on stakes versus plants grown without stakes were compared. the ...	2017	29131625
effect of seyoeum on obesity, insulin resistance, and nonalcoholic fatty liver disease of high-fat diet-fed c57bl/6 mice.	this study was performed to evaluate the effect of seyoeum (sye), a novel herbal meal replacement, on insulin resistance and nonalcoholic fatty liver disease (nafld) in obese mice fed with a high-fat diet (hfd).	2017	29085434
hepatic anti-inflammatory effect of hexane extracts of dioscorea batatas decne: possible suppression of toll-like receptor 4-mediated signaling.	the hepatic anti-inflammatory potential of hexane extracts of dioscorea batatas decne edible part (edh-1e) and bark (edh-2b) were investigated in western-type diet-fed apolipoprotein e null [apoe (-/-)] mice and hepg2 cells. edh-1e and edh-2b suppressed the increased levels of tumor necrosis factor alpha (tnf-α), interleukin (il)-6, transforming growth factor beta 1 (tgf-β1), vascular cell adhesion protein 1 (vcam-1), and monocyte chemoattractant protein-1 (mcp-1), and reduced infiltration of mo ...	2017	28538192
the complete genome sequence of a novel virus, bellflower veinal mottle virus, suggests the existence of a new genus within the family potyviridae.	a new virus was isolated from a bellflower (campanula takesimana) plant showing veinal mottle symptoms, and its complete genome sequence was determined. the viral genome consists of a positive-sense single-stranded rna of 8,259 ribonucleotides. electron microscopic observation revealed that the viral genome is packaged as a filamentous particle with an average length of approximately 760 nm. blast searches of protein databases showed that the encoded polyprotein has a maximum amino acid sequence ...	2017	28434099
diosgenin-rich yam extract enhances cognitive function: a placebo-controlled, randomized, double-blind, crossover study of healthy adults.	diosgenin, a yam-derived compound, was found to facilitate the repair of axonal atrophy and synaptic degeneration and improve memory dysfunction in a transgenic mouse model of alzheimer's disease (ad). it was also found to enhance neuronal excitation and memory function even in normal mice. we hypothesized that diosgenin, either isolated or in an extract, may represent a new category of cognitive enhancers with essential activities that morphologically and functionally reinforce neuronal network ...	2017	29064406
new bioactive compounds from aquatic endophyte chaetomium globosum.	two new oxidation products-related aureonitol and cytochalasan were isolated from chaetomium globosum fermented in chinese yam (dioscorea opposita) and determined as 10,11-dihydroxyl- aureonitol (1) and yamchaetoglobosin a (2). compound 2 indicated significant inhibitory effect on nitric oxide production in lps-activated macrophages, anti-acetylcholinesterase activity with the inhibition ratios of 92.5, 38.2% at 50 μm, and cytotoxicity to hl-60, a-549, smmc-7721, mcf-7 and sw480 with the range o ...	2017	28927295
hypoglycemic effect of chinese yam (dioscorea opposita rhizoma) polysaccharide in different structure and molecular weight.	three new chinese yam polysaccharides (namely hsy, huaishanyao in chinese) were isolated using the methods of boiled water extraction and stepwise ethanolic precipitation, combined with the tangential flow ultrafiltration membrane system. their molecular weights were determined by high performance gel permeation chromatography. three type yam polysaccharides in different molecular weight were isolated: hsy-i (>50 kda), hsy-ii (10 to 50 kda), hsy-iii (<10 kda). the monosaccharide and glycosidic b ...	2017	28906544
immunomodulatory activity of the glycoprotein isolated from the chinese yam (dioscorea opposita thunb).	the yam (dioscorea opposita thunb) is a well-known edible food and widely used as the traditional chinese medicine. the present investigation was designed to evaluate the immunomodulatory activity of glycoprotein (dot) from yam and explore its possible molecular mechanisms. results showed that the dot could improve the cell immunity, humoral immunity and phagocytic system function of the normal mice. the dot could also increase the production of tnf-α, interleukin-6 and nitric oxide and enhance ...	2017	28840617
exploring the immunopotentiation of chinese yam polysaccharide poly(lactic-co-glycolic acid) nanoparticles in an ovalbumin vaccine formulation in vivo.	biocompatible and biodegradable poly(lactic-co-glycolic acid) (plga) has been approved by the us food and drug administration and has frequently been used to develop potential vaccine delivery systems. the immunoregulation and immunopotentiation of chinese yam polysaccharide (cyp) have been widely demonstrated. in the current study, cell uptake mechanisms in dendritic cells (dcs) were monitored in vitro using confocal laser scanning microscopy, transmission electron microscopy, and flow cytometr ...	2017	28776443
estrogenic effects of the extracts from the chinese yam (dioscorea opposite thunb.) and its effective compounds in vitro and in vivo.	the aim of this study was to explore the estrogenic effects of the extracts from chinese yam and its effective compounds.	2018	29360751
functional properties of chinese yam (dioscorea opposita thunb. cv. baiyu) soluble protein.	in this study, yam soluble protein (ysp) was extracted from chinese yam (dioscorea opposita thunb. cv. baiyu) and the functional properties were investigated under the influence of ph and ionic strength. as results, ysp was highly soluble and had better emulsifying activity over a wide range of ph. the solubility of ysp decreased in 0.5 and 1.0 m nacl solution. an increment in nacl concentration reduced the emulsion activity index and emulsion stability index of ysp. the oil absorption capacity ...	2018	29358831
characterisation of the mucilage polysaccharides from dioscorea opposita thunb. with enzymatic hydrolysis.	the mucilage polysaccharides from dioscorea opposita (domp) were extracted and treated with a single/dual enzymatic hydrolysis. the characterisation and viscosity were subsequently investigated in this study. domp obtained 62.52% mannose and 23.45% glucose. after single protease and trichloroacetic acid (tca) treatments, the mannose content was significantly reduced to 3.96%, and glucose increased from 23.45% to 45.10%. dual enzymatic hydrolysis also decreased the mannose and glucose contents to ...	2018	29287353