Publications

use of commercial plant species in a hydroponic system to treat domestic wastewaters.	the objectives in this work were to investigate a conceptual layout for an inexpensive and simple system that would treat primary municipal wastewater to discharge standards. a commercial hydroponic system was adapted for this study and the wastewater was used to irrigate wooly digitalis (digitalis lanata ehrh.) and foxglove (digitalis purpurea l.). these plants are medicinal and produce cardenolide compounds. influent and effluent samples were collected once a month for six months and analyzed ...	2013	15074822
[preparation of biologically transformed raw material from woolly foxglove digitalis lanata ehrh and isolation of digoxin from it].	a biotechnological approach is proposed for conservation of a terraneous part of woolly foxglove under anaerobic conditions with a subsequent air-sun drying of the biologically transformed raw material. during the conservation primary foxglove glycosides completely convert to secondary ones which do not transform further. a simple method is described for preparation from the transformed raw material of an enriched glycoside fraction with the yield of 3.6% and for isolation from this fraction of ...	2013	14593875
clinical utility of plasma digoxin measurements.	the results of plasma digoxin concentration (pdc) measurements in 260 patients treated with digitalis lanata preparations (digoxin, lanatoside c, betamethyl-digoxin) allowed the following observations: 1. owing to an important overlap between "toxic" and "nontoxic" pdc values, the method has a limited value in the diagnosis of digitalis toxicity. however, in patients with toxicity associated with pdc under 2 ng/ml, it allows identification digitalis sensitivity. 2. a dosage selection based on pd ...	2013	2749155
[stability and technology of ampule solutions of cardenolides of the wooly foxglove (digitalis lanata ehrh.)].		2014	862945
[spectrophotometric method for a quantitative determination of lanatosides a, b, c in the leaves of digitalis lanata].		2014	5093349
[biochemical study of digitalis (digitalis lanata ehrb.)].		2014	5721787
truncation of n-terminal regions of digitalis lanata progesterone 5β-reductase alters catalytic efficiency and substrate preference.	n-terminal truncated forms of progesterone 5β-reductase (p5βr) were synthesized taking a full-length cdna encoding for digitalis lanata p5βr with a hexa-histidine tag attached at the c-terminus (rdlp5βrc) as the starting point. four petite-c-his/dlp5βr constructs coding for p5βr derivatives truncated in the n-terminal region, termed rdlp5βrcn-10, rdlp5βrcn-20, rdlp5βrcn-30, and rdlp5βrcn-40 were obtained by site-directed mutagenesis. the cdnas coding for full-length rdlp5βrc, rdlp5βrcn-10 and rd ...	2014	24370479
progesterone 5β-reductase genes of the brassicaceae family as function-associated molecular markers.	this study aimed to define progesterone 5β-reductases (p5βr, ec 1.3.99.6, enone 1,4-reductases) as function-associated molecular markers at the plant family level. therefore cdnas were isolated from 25 brassicaceae species, including two species, erysimum crepidifolium and draba aizoides, known to produce cardiac glycosides. the sequences were used in a molecular phylogeny study. the cladogram created is congruent to the existing molecular analyses. recombinant his-tagged forms of the p5βr cdnas ...	2015	26108256
purification of δ(5)-3-ketosteroid isomerase from digitalis lanata.	the isomerization of 5-pregnene-3,20-dione into 4-pregnene-3,20-dione was investigated to shed further light on cardenolide biosynthesis and to characterize the enzymes involved in cardenolide formation. it was shown that the δ(5)-3-ketosteroid isomerase of digitalis lanata, which catalyzes this isomerization, is an individual enzyme and not, as previously thought, associated with δ(5)-3β-hydroxysteroid dehydrogenase. the enzyme was purified by fractionated ammonium sulfate precipitation, hydrop ...	2015	25468533
progesterone 5β-reductases/iridoid synthases (prise): gatekeeper role of highly conserved phenylalanines in substrate preference and trapping is supported by molecular dynamics simulations.	vein patterning 1 (vep1)-encoded progesterone 5β-reductases/iridoid synthases (prise) belong to the short-chain dehydrogenase/reductase superfamily of proteins. they are characterized by a set of highly conserved amino acids in the substrate-binding pocket. all prises are capable of reducing the activated c=c double bond of various enones enantioselectively and therefore have a potential as biocatalysts in bioorganic synthesis. here, recombinant forms of prises of arabidopsis thaliana and digita ...	2016	26457532
[steroid saponins with more than one sugar chain. vi. lanatigoside and lanagitoside, two bisdesmosidic 22-hydroxyfurostanol glycosides from the leaves of digitalis lanata ehrh].		2016	5077133
lanatoside c, a cardiac glycoside, acts through protein kinase cδ to cause apoptosis of human hepatocellular carcinoma cells.	recent studies have revealed that cardiac glycosides, such as digitalis and digoxin, have anticancer activity and may serve as lead compounds for the development of cancer treatments. the poor prognosis of hepatocellular carcinoma (hcc) patients reflects the development of resistance to current chemotherapeutic agents, highlighting the need for discovering new small-molecule therapeutics. here, we found that lanatoside c, an anti-arrhythmic agent extracted from digitalis lanata, inhibited the gr ...	2017	28387249