Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| trends in aging and skin care: ayurvedic concepts. | the association between ayurveda, anti-aging and cosmeceuticals is gaining importance in the beauty, health and wellness sector. ayurvedic cosmeceuticals date back to the indus valley civilization. modern research trends mainly revolve around principles of anti-aging activity described in ayurveda: vayasthapana (age defying), varnya (brighten skin-glow), sandhaniya (cell regeneration), vranaropana (healing), tvachya (nurturing), shothahara (anti-inflammatory), tvachagnivardhani (strengthening sk ... | 2010 | 21836797 |
| two new triterpene glycosides from centella asiatica. | phytochemical investigation of the leaves of centella asiatica resulted in the isolation and characterization of eight triterpenes and/or saponins [which were characterized as 23- o-acetylmadecassoside (1), asiatic acid (2), madecassic acid (3), asiaticoside c (4), asiaticoside f (5), asiaticoside (6), madecassoside (7), and 23- o-acetylasiaticoside b (8)] together with sitosterol 3-o-beta-glucoside ( 9), stigmasterol 3- o- beta-glucoside (10), and querectin-3- o-beta-d-glucuronide (11). a new u ... | 2010 | 20143295 |
| contact dermatitis due to centella asiatica. | 2010 | 20136880 | |
| characterisation and immuno-stimulating activity of polysaccharides from thai medicinal plants. | water-soluble polysaccharides were isolated from the tubers of butea superba roxb. and pueraria candollei wall. ex benth. var. mirifica (shaw et suvat.) c. niyomdham, the leaves of centella asiatica (l.) urb, ocimum basilicum l., psidium guajava and andrographis paniculata (burn. f.) nees, the stems of cymbopogon citratus (stapf exg), and the fruits of psidium guajava and scaphium scaphigerum. the immunological impacts of the polysaccharides on t-lymphocyte proliferation in vitro was investigate ... | 2010 | 20812129 |
| carotenoid content and in vitro bioaccessibility of lutein in some leafy vegetables popular in sri lanka. | lutein is one of the major carotenoids in green leafy vegetables (glvs), which show a marked antioxidant activity. the study was carried out to identify and quantify the carotenoid content of selected sri lankan glvs. rp-hplc with photodiode array detection method was followed for the separation, identification and quantification of carotenoids. among the vegetables analyzed, ipomoea batatas leaves showed the highest beta-carotene content (743.9+/-35.0 microg/g dry weight (dw)) while syngonium a ... | 2010 | 20651462 |
| pharmacological review on centella asiatica: a potential herbal cure-all. | in recent times, focus on plant research has increased all over the world. centella asiatica is an important medicinal herb that is widely used in the orient and is becoming popular in the west. triterpenoid, saponins, the primary constituents of centella asiatica are manly believed to be responsible for its wide therapeutic actions. apart from wound healing, the herb is recommended for the treatment of various skin conditions such as leprosy, lupus, varicose ulcers, eczema, psoriasis, diarrhoea ... | 2010 | 21694984 |
| the efficacy and side effects of oral centella asiatica extract for wound healing promotion in diabetic wound patients. | to study clinical efficacy and side effects of the oral centella asiatica extract capsule in the diabetic wound healing. and to study the side effects of centella asiatica extract capsule. | 2010 | 21298840 |
| allergic contact dermatitis due to centella asiatica: a new case. | we report a new case of allergic contact dermatitis due to centella asiatica, a weak sensitizer. patch tests with centella asiatica at different vehicles and in different concentrations were carried out. regarding our results, we suggest as a routine procedure to start with 1% pet, and when the test remains negative 10% pet and 2% eth 70 degrees could be used. | 2010 | 8766746 |
| pentacyclic triterpenoids from the medicinal herb, centella asiatica (l.) urban. | centella asiatica accumulates large quantities of pentacyclic triterpenoid saponins, collectively known as centelloids. these terpenoids include asiaticoside, centelloside, madecassoside, brahmoside, brahminoside, thankuniside, sceffoleoside, centellose, asiatic-, brahmic-, centellic- and madecassic acids. the triterpene saponins are common secondary plant metabolites and are synthesized via the isoprenoid pathway to produce a hydrophobic triterpenoid structure (aglycone) containing a hydrophili ... | 2009 | 19924039 |
| an improved hplc-uv method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of centella asiatica (l.) urb (apiaceae). | the simultaneous quantification of madecassoside, asiaticoside, madecassic acid and asiatic acid in centella asiatica by hplc-uv is proposed. asiaticoside was used as reference for the quantification of heterosides and asiatic acid for aglycones. the evaluation of the extraction efficiency of the four molecules led to use soxhlet extraction for 8 h. the method was validated and was found to be accurate in the concentration range of 1.0-3.0 mg/ml for asiaticoside and 0.5-2.0 mg/ml for asiatic aci ... | 2009 | 19349219 |
| chemical constituents of centella asiatica. | studies on the chemical constituents of the aerial part of centella asiatica have led to the isolation of three new compounds, named centellin (1), asiaticin (2), and centellicin (3). their structures have been elucidated through spectral studies including 2d nmr experiments (hmqc, hmbc, (1)h-(1)h cosy, noesy and j resolved). | 2009 | 17613628 |
| anti-allergic, anti-pruritic, and anti-inflammatory activities of centella asiatica extracts. | this study investigated antipruritic and anti-inflammatory effect of centella asiatica extract in rats and anti-allergic in vitro using sheep (capra hircus) serum method and compound 48/80 induced mast cell degranulation method, compared with standard drug ketotifen fumarate. in rats, extract of centella asiatica administered orally was examined for anti-pruritic study and chlorpheniramine maleate was used as standard drug while carageenan paw induced inflammatory method was used for the antiinf ... | 2009 | 20606777 |
| madecassoside attenuates inflammatory response on collagen-induced arthritis in dba/1 mice. | madecassoside (ma), a triterpenoid product isolated from centella asiatica, has been described to exhibit antioxidant and anti-inflammatory activities. the present study was undertaken to determine whether madecassoside (ma) is efficacious against collagen-induced arthritis (cia) in mice and its possible mechanisms. dba/1j mice were immunized with bovine type ii collagen and treated with ma (3, 10 and 30 mg/kg d, i.g.) from days 21 to 42 after immunization. arthritis was evaluated by hind paw sw ... | 2009 | 19135346 |
| determination of asiatic acid in beagle dog plasma after oral administration of centella asiatica extract by precolumn derivatization rp-hplc. | a novel precolumn derivatization reversed-phase high-performance liquid chromatography (rp-hplc) method with uv-vis detection for the quantitative determination of total concentration of asiatic acid (aa) in beagle dog plasma is described. aa was extracted with n-hexane-dichloromethane-2-propanol (20:10:1, v/v/v) from plasma, which had been hydrolyzed by acid and derivatized with p-toluidine. chromatographic separation was achieved on a c(18) column using gradient elution in a water-methanol sys ... | 2009 | 19167274 |
| in vitro and in vivo modulation of cartilage degradation by a standardized centella asiatica fraction. | osteoarthritis (oa) is a degenerative joint disease in which focal cartilage destruction is one of the primary features. the present study aims to evaluate the effect of a centella asiatica fraction on in vitro and in vivo cartilage degradation. bovine cartilage explants and bovine chondrocytes cultured in alginate were stimulated with il-1 beta in the presence or absence of different concentrations (2, 5 and 10 microg/ml) of a standardized centella asiatica triterpenes (cat) fraction. the cat f ... | 2009 | 19307458 |
| modulation of lipid metabolism by centella asiatica in oxidative stress rats. | a study was carried out to investigate the effects of centella asiatica leaf on lipid metabolism of oxidative stress rats. the rats were fed 0.1% hydrogen peroxide (h(2)o(2)) with either 0.3% (w/w) c. asiatica extract, 5%c. asiatica powder (w/w), or 0.3% (w/w) alpha-tocopherol for 25 wk. results of the study showed that c. asiatica powder significantly (p < 0.05) lowered serum low-density lipoprotein compared to that of control rats (rats fed h(2)o(2) only). at the end of the study c. asiatica-f ... | 2009 | 19323754 |
| asiatic acid, a pentacyclic triterpene from centella asiatica, is neuroprotective in a mouse model of focal cerebral ischemia. | asiatic acid, a triterpenoid derivative from centella asiatica, has shown biological effects such as antioxidant, antiinflammatory, and protection against glutamate- or beta-amyloid-induced neurotoxicity. we investigated the neuroprotective effect of asiatic acid in a mouse model of permanent cerebral ischemia. various doses of asiatic acid (30, 75, or 165 mg/kg) were administered orally at 1 hr pre- and 3, 10, and 20 hr postischemia, and infarct volume and behavioral deficits were evaluated at ... | 2009 | 19382233 |
| a new herbal combination, etana, for enhancing erectile function: an efficacy and safety study in animals. | we present herein a new herbal combination called etana that is composed of five herbal extracts including panax quinquelotius (ginseng), eurycoma longifolia (tongkat ali), epimedium grandiflorum (horny goat weed), centella asiatica (gotu kola) and flower pollen extracts. most of the above-mentioned extracts have a long historical and traditional use for erectile dysfunction (ed). on the basis of the mechanism of action of each of the above, a combination is introduced to overcome several physio ... | 2009 | 19494825 |
| protection of dna and membranes from gamma-radiation induced damages by centella asiatica. | the objective of the present study was to examine the ability of centella asiatica extract to offer protection to dna and membranes against the deleterious effects of ionizing radiation exposure. | 2009 | 19589237 |
| asiatic acid induces colon cancer cell growth inhibition and apoptosis through mitochondrial death cascade. | cancer is one of the leading causes of death in the world. the triterpenoid compound asiatic acid derived from the tropical medicinal plant centella asiatica displays cytotoxic activity on fibroblast cells and several other kinds of cells. the present work studies asiatic acid-mediated growth inhibition of cancer cells and the underlying mechanism. asiatic acid markedly inhibited cancer cell proliferation. apoptosis of sw480 human colon cancer cells was induced by asiatic acid as shown by flow c ... | 2009 | 19652380 |
| characterization of a dammarenediol synthase in centella asiatica (l.) urban. | to elucidate the exact function of cabas in centella asiatica, which was previously reported as a putative beta-amyrin synthase [plant cell rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). after inducing the cabas gene with galactose, a peak consistent with the dammarenediol standard was detected in lc/apcims analyses and the accumulated product was confirmed as dammarenediol. cabas should therefore be renamed to c. asiatica d ... | 2009 | 19796960 |
| adulticidal and larvicidal efficacy of some medicinal plant extracts against tick, fluke and mosquitoes. | the adulticidal and larvicidal effect of indigenous plant extracts were investigated against the adult cattle tick haemaphysalis bispinosa neumann, 1897 (acarina: ixodidae), sheep fluke paramphistomum cervi zeder, 1790 (digenea: paramphistomatidae), fourth instar larvae of malaria vector, anopheles subpictus grassi and japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae). the aim of this study was to evaluate the toxic effect of leaf hexane, chloroform, ethyl acetate, ... | 2009 | 19819626 |
| antioxidant and cytotoxic activities of centella asiatica (l) urb. | in the present study, the phenolic (folin-dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (dpph) assay] and cytotoxic activities of an aqueous extract (ae) of centella asiatica leaves were investigated. the aqueous extract (50 g/l) was obtained by infusion followed by cold maceration for 24 h. the levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. the ae showed elevated dpph scavenging activity ... | 2009 | 19865514 |
| larvicidal and adulticidal activities of some medicinal plants against the malarial vector, anopheles stephensi (liston). | the present study was undertaken to test the efficacy of 11 commonly available medicinal plants and compare its efficacy in relation to larvicidal and mosquitocidal activities against larvae and adults of anopheles stephensi (liston). all the medicinal plants and the mixture were effective against larvae of a. stephensi as evidenced by low lethal concentration and lethal time. the lethality varied in adults and plant extracts of mixture; eucalyptus globulus, cymbopogan citratus, artemisia annua, ... | 2009 | 18787842 |
| an in vitro investigation of herbs traditionally used for kidney and urinary system disorders: potential therapeutic and toxic effects. | renal fibrosis is central to progression of most chronic renal pathologies. antioxidants that protect the tubular epithelium and anti-fibrotics that induce apoptosis of pro-fibrotic myofibroblasts without adversely affecting tubular epithelium may slow progression of renal fibrosis, while toxic substances may exacerbate renal scarring. we investigated 47 herbs for their in vitro toxic or antioxidant effects on normal renal mammalian fibroblasts (nrk49f) and tubular epithelial cells (nrk52e) to d ... | 2009 | 18808387 |
| enhancement of amygdaloid neuronal dendritic arborization by fresh leaf juice of centella asiatica (linn) during growth spurt period in rats. | centella asiatica (cea) is a creeping herb, growing in moist places in india and other asian countries. ayurvedic system of medicine, an alternate system of medicine in india, uses leaves of cea for memory enhancement. here, we have investigated the role of cea fresh leaf juice treatment during growth spurt period of rats on dendritic morphology of amygdaloid neurons, one of the regions concerned with learning and memory. the present study was conducted on neonatal rat pups. the rat pups (7-days ... | 2009 | 18955230 |
| studies on indian green leafy vegetables for their antioxidant activity. | to identify the potential of green leafy vegetables (glv) as antioxidants, methanolic extracts of amaranthus sp., centella asiatica, murraya koenigii and trigonella foenum graecum were studied for their antioxidant activity in different systems at multiple concentrations. total antioxidant activity assessed by phosphomolybdenum method, free radical scavenging activity by 1,1-diphenly-2-picryl hydrazyl (dpph), reducing power and ferrous ion chelating activity were determined. the glv were analyze ... | 2009 | 18985454 |
| neuroprotective effects of centella asiatica against intracerebroventricular colchicine-induced cognitive impairment and oxidative stress. | oxidative stress appears to be an early event involved in the pathogenesis of alzheimer's disease. the present study was designed to investigate the neuroprotective effects of centella asiatica against colchicine-induced memory impairment and oxidative damage in rats. colchicine (15 mug/5 mul) was administered intracerebroventricularly in the lateral ventricle of male wistar rats. morris water maze and plus-maze performance tests were used to assess memory performance tasks. various biochemical ... | 2009 | 20798885 |
| centella asiatica extract selectively decreases amyloid beta levels in hippocampus of alzheimer's disease animal model. | psapp mice expressing the 'swedish' amyloid precursor protein and the m146l presenilin 1 mutations are a well-characterized model for spontaneous amyloid beta plaque formation. centella asiatica has a long history of use in india as a memory enhancing drug in ayurvedic literature. the study investigated whether centella asiatica extract (cae) can alter the amyloid pathology in psapp mice by administering cae (2.5 or 5.0 g/kg/day) starting at 2 months of age prior to the onset of detectable amylo ... | 2009 | 19048607 |
| comparison of the effects of collagenase and extract of centella asiatica in an experimental model of wound healing: an immunohistochemical and histopathological study. | in this study, we compared the effects of collagenase and centella asiatica in the rat model. twenty-seven female rats were divided into three groups, and two full-thickness wounds were made for each animal. collagenase ointment was applied topically to group i and c. asiatica ointment to group ii rats. in group iii, no treatment was applied. on the third day of treatment, wounds on the left side of three animals of each group were excised. on the fifth and eighth day of the treatments, the same ... | 2008 | 19128262 |
| apoptosis induction of centella asiatica on human breast cancer cells. | the present study evaluated the ability of methanolic extract of centella asiatica (linn) urban (umbelliferae) to induce apoptosis in different cancer cell lines. mcf-7 cells emerged as the most sensitive cell line for in vitro growth inhibitory activity. c. asiatica extract induced apoptosis in mcf-7 cells as indicated by nuclear condensation, increased annexin staining, loss of mitochondrial membrane potential and induction of dna breaks identified by tunel reactivity. it is possible that the ... | 2008 | 20162036 |
| mosquito larvicidal activity of oleic and linoleic acids isolated from citrullus colocynthis (linn.) schrad. | in mosquito control programs, botanical origin may have the potential to be used successfully as larvicides. the larvicidal activity of crude acetone, hexane, ethyl acetate, methanol, and petroleum ether extracts of the leaf of centella asiatica linn., datura metal linn., mukia scabrella arn., toddalia asiatica (linn.) lam, extracts of whole plant of citrullus colocynthis (linn.) schrad, and sphaeranthus indicus linn. were assayed for their toxicity against the early fourth instar larvae of cule ... | 2008 | 18688644 |
| protective antioxidant effect of centella asiatica bioflavonoids on lead acetate induced neurotoxicity. | lead (pb) is a neurotoxic heavy metal and children in the developmental stage are particularly susceptible to toxic effects of lead exposure. the brain is the key organ involved in interpreting and responding to potential stressors. epidemiological investigations have established the relationship between chronic lead exposure and cognitive impairments in young children. excessive production of radical species plays an important role in neuronal pathology resulting from excitotoxic insults, there ... | 2008 | 19025005 |
| [molecular characters of centella asiatica found with rapd technology]. | to analyze the dna molecular characters of centella asiatica with rapd technology. | 2008 | 18973007 |
| prophylactic neuroprotective property of centella asiatica against 3-nitropropionic acid induced oxidative stress and mitochondrial dysfunctions in brain regions of prepubertal mice. | despite the increasing popularity of centella asiatica (a well known plant in ayurvedic medicine) globally, evidence demonstrating its protective efficacy against neurotoxicants in animal models is limited. 3-nitropropionic acid (3-npa), a fungal toxin is a well known neurotoxicant which induces selective striatal pathology similar to that seen in huntington's disease. the present study aimed to understand the neuroprotective efficacy of a standardized aqueous extract of c. asiatica (ca) against ... | 2008 | 18930762 |
| antigenotoxic role of centella asiatica l. extract against cyproterone acetate induced genotoxic damage in cultured human lymphocytes. | the majority of the indian population use traditional natural preparations derived from plant material for the treatment of various diseases, and for that reason it becomes necessary to assess the mutagenic potential or modulating action of plants extract when associated with other substances. the genotoxicity testing provides human a risk assessment. earlier in vitro and in vivo studies reveal that the plant extracts from various parts of the plant play a modulating role in xenobiotic effects. ... | 2008 | 17719740 |
| enhancement of hippocampal ca3 neuronal dendritic arborization by centella asiatica (linn) fresh leaf extract treatment in adult rats. | centella asiatica (cea) is a creeper, growing in moist places in india and other asian countries. leaves of cea are used for memory enhancement in the ayurvedic system of medicine, an alternate system of medicine in india. in the present study, we investigated the role of cea fresh leaf extract treatment on the dendritic morphology of hippocampal ca3 neurons, one of the regions concerned with learning and memory, in adult rats. | 2008 | 18218554 |
| inhibition of lps-induced no and pge2 production by asiatic acid via nf-kappa b inactivation in raw 264.7 macrophages: possible involvement of the ikk and mapk pathways. | in the present study, we investigated the effect of asiatic acid (the aglycon of asiaticoside) and asiaticoside isolated from the leaves of centella asiatica (umbelliferae) on lps-induced no and pge(2) production in raw 264.7 macrophage cells. asiatic acid more potently inhibited lps-induced no and pge(2) production than asiaticoside. consistent with these observations, the protein and mrna expression levels of inducible inos and cox-2 enzymes were inhibited by asiatic acid in a concentration-de ... | 2008 | 18279797 |
| madecassoside reduces ischemia-reperfusion injury on regional ischemia induced heart infarction in rat. | madecassoside (ma), one of the principle terpenoids in centella asiatica, has shown protect effect on isolated rat hearts and isolated cardiomyocytes against reperfusion injury in our previous studies. the aim of this study is to investigate if ma also protected against myocardial ischemia-reperfusion injury in vivo. the ischemia infarction model was established in rats. left ventricular function was monitored during the ischemia-reperfusion period by a multi-channel recorder. after the ischemia ... | 2008 | 18310910 |
| gotu kola (centella asiatica) extract enhances phosphorylation of cyclic amp response element binding protein in neuroblastoma cells expressing amyloid beta peptide. | alzheimer's disease (ad) is a progressive neurodegenerative disorder that shows cognitive deficits and memory impairment. extract from the leaves of gotu kola (centella asiatica) have been used as an alternative medicine for memory improvement in indian ayurvedic system of medicine for a long time. although several studies have revealed its effect in ameliorating the cognitive impairment in rat models of ad and stimulating property on neuronal dendrites of hippocampal region, the molecular mecha ... | 2008 | 18431001 |
| preparation and characterization of herbal creams for improvement of skin viscoelastic properties. | the aim of this study was to formulate and evaluate herbal cosmetic creams for their improvement of skin viscoelastic and hydration properties. the cosmetic cream formulations were designed by using ethanolic extracts of glycyrriza glabra, curcuma longa (roots), seeds of psorolea corlifolia, cassia tora, areca catechu, punica granatum, fruits of embelica officinale, leaves of centella asiatica, dried bark of cinnamon zeylanicum and fresh gel of aloe vera in varied concentrations (0.12-0.9%w/w) a ... | 2008 | 18452435 |
| centella asiatica water extract inhibits ipla2 and cpla2 activities in rat cerebellum. | centella asiatica (l.) urb an is distributed widely in south america and asia and is known as a therapeutic agent in folk medicine, capable of improving memory and treating several neurological disorders. asiaticoside is one of the compounds found in c. asiatica leaves that is suggested to be responsible for its pharmacological potential. phospholipase a(2) (pla(2)) is a group of enzymes that has abnormal activity in the central nervous system in some neuropsychiatric diseases. in this work, the ... | 2008 | 18455381 |
| madecassoside isolated from centella asiatica herbs facilitates burn wound healing in mice. | the current study was designed to investigate the effect of madecassoside, the major triterpene in centella asiatica, on burn wound healing and its possible mechanism of action. an oral administration of madecassoside (6, 12, 24 mg/kg) facilitated wound closure in a time-dependent manner and reached its peak effect, nearly completely wound closure, on day 20 in the group receiving the highest dose of 24 mg/kg of madecassoside. further histopathological analysis revealed that madecassoside allevi ... | 2008 | 18484522 |
| clinical, biometric and structural evaluation of the long-term effects of a topical treatment with ascorbic acid and madecassoside in photoaged human skin. | skin ageing is a complex process determined by the genetic endowment of individual and environmental factors, such as sun exposure. the effects of skin ageing are mostly encountered in the superficial dermis and in the epidermis. we have previously demonstrated in vivo the beneficial effect of a topically applied formula of 5% vitamin c in the treatment of skin ageing. another active compound, madecassoside extracted from centella asiatica, known to induce collagen expression and/or to modulate ... | 2008 | 18503551 |
| effect of centella asiatica leaf powder on oxidative markers in brain regions of prepubertal mice in vivo and its in vitro efficacy to ameliorate 3-npa-induced oxidative stress in mitochondria. | centella asiatica (ca) is a common medicinal plant used in the ayurvedic system of medicine to treat various ailments and as a memory enhancer. despite its extensive usage in children, data on its ability to modulate neuronal oxidative stress in prepubertal rodents are limited. hence in the present study we have addressed primarily two questions (i) whether dietary intake of ca leaf powder possess the propensity to modulate endogenous oxidative markers in mouse brain regions and (ii) the efficac ... | 2008 | 18539017 |
| plant-derived micronutrients suppress monocyte adhesion to cultured human aortic endothelial cell layer by modulating its extracellular matrix composition. | monocyte adhesion to endothelium plays an important role in atherosclerosis. we investigated the effects of micronutrients on monocyte-binding properties of extracellular matrix (ecm) produced by human aortic endothelial cells (aoec). confluent cultures of aoec were exposed to ascorbic acid, quercetin, gotu kola extract (10% asiatic acid), green tea extract (40% epigallocatechin gallate), or a mixture of these micronutrients for 48 hours. aoec-produced ecm was exposed by differential treatment. ... | 2008 | 18594473 |
| anti-rheumatoid arthritic effect of madecassoside on type ii collagen-induced arthritis in mice. | madecassoside is the highest amount of triterpene constituent in centella asiatica herbs, a frequently prescribed crude drug in southeastern asian and china for wound healing and scar management. the present study aimed to investigate the therapeutic potential and underlying mechanisms of madecassoside on collagen ii (cii)-induced arthritis (cia) in mice. madecassoside (10, 20 and 40mg/kg), orally administered from the day of the antigen challenge for twenty consecutive days, dose-dependently al ... | 2008 | 18652917 |
| endophytic fungi from leaves of centella asiatica: occurrence and potential interactions within leaves. | fungal endophytes were isolated from leaves of centella asiatica (apiaceae) collected at mangoro (middle eastern region of madagascar, 200 km from antananarivo). forty- five different taxa were recovered. the overall foliar colonization rate was 78%. the most common endophytes were the non-sporulating species 1 (isolation frequency if 19.2%) followed by colletotrichum sp.1 (if 13.2%), guignardia sp. (if 8.5%), glomerella sp. (if 7.7%), an unidentified ascomycete (if 7.2%), the non-sporulating sp ... | 2008 | 17610142 |
| neuronutrient impact of ayurvedic rasayana therapy in brain aging. | ayurveda is the oldest system of medicine in the world, its antiquity going back to the vedas. it adapts a unique holistic approach to the entire science of life, health and cure. the areas of special consideration in ayurveda are geriatrics, rejuvenation, nutrition, immunology, genetics and higher consciousness. the ayurvedic texts describe a set of rejuvenative measures to impart biological sustenance to the bodily tissues. these remedies are called rasayana which are claimed to act as micronu ... | 2008 | 18931935 |
| [preparation of asiaticoside and madecassoside from the extract of centella asiatica (l.) urb using preparative high performance liquid chromatography]. | a high performance liquid chromatographic (hplc) method was successfully used to separate asiaticoside and madecassoside from the extract of centella asiatica (l.) urb. the raw material containing 20% asiaticoside and 45% madecassoside was dissolved in methanol and used for the preparation of the pure compounds. the flow rate of the mobile phase, injection volume and detection wavelength were optimized. the optimum operation parameters were then selected as follows: a c18 column (50 mm x 200 mm, ... | 2008 | 18724677 |
| triterpenoid saponin content and the expression level of some related genes in calli of centella asiatica. | centella asiatica has been extensively studied but there has been no report to date that relates gene expression and centelloside production in non-differentiated tissues. we have determined the content of the four principal triterpenoid bioactive compounds of c. asiatica (asiaticoside, madecassoside, asiatic acid and madecassic acid) in calli grown in different media and checked the expression level of some of the genes in the centelloside biosynthetic pathway. the results when compared with da ... | 2008 | 18575812 |
| enrichment and purification of madecassoside and asiaticoside from centella asiatica extracts with macroporous resins. | in present study, the performance and separation characteristics of five macroporous resins for the enrichment and purification of asiaticoside and madecassoside from centella asiatica extracts have been evaluated. the adsorption and desorption properties of total triterpene saponins (80% purity) on macroporous resins including hpd100, hpd300, x-5, ab-8 and d101 have been compared. according to our results, hpd100 offered higher adsorption and desorption capacities and higher adsorption speed fo ... | 2008 | 18457845 |
| two new flavonoids from centella asiatica (linn.). | two new flavonoids named castilliferol 1 and castillicetin 2, as well as a known compound, isochlorogenic acid 3, were isolated from the whole plant of centella asiatica. isolates 1 and 2 exhibited good antioxidant activity using 2,2-diphenyl-1-picryl hydrazyl radical solution with ic(50) values of 23.10 and 13.30, respectively. the structures of these isolates were determined by analytical and spectral data, including 1-d and 2-d nmr spectra. | 2008 | 18404308 |
| hepatotoxic slimming aids and other herbal hepatotoxins. | perceptions of safety and/or cultural mores prompt individuals to seek herbal slimming aids in preference to conventional dietary, physical activity and medication-based protocols. in recent years, terpenoid-containing dietary supplements have been implicated in causing severe and sometimes fatal hepatotoxicity. teucrium polium (germander) was the first of these herbal products to be clearly linked to cases of acute liver failure. subsequently, similar hepatotoxicity has been observed with other ... | 2008 | 18318821 |
| positive modulation of cognition and mood in the healthy elderly volunteer following the administration of centella asiatica. | centella asiatica has a reputation to restore decline cognitive function in traditional medicine and in animal model. however, little evidence regarding the efficacy of centella asiatica from systematized trials is available. therefore, the present randomized, placebo-controlled, double-blind study investigated the effect of centella asiatica on cognitive function of healthy elderly volunteer. | 2008 | 18191355 |
| electrospun gelatin fiber mats containing a herbal-centella asiatica-extract and release characteristic of asiaticoside. | ultra-fine gelatin (type a, porcine skin, ∼180 bloom) fiber mats containing a methanolic crude extract of centella asiatica (l.) urban, a medicinal plant widely known for its traditional medical applications including its wound healing ability, were fabricated, for the first time, from the neat gelatin solution (22% w/v in 70 vol% acetic acid) containing the crude extract (mca) in various amounts (i.e. 5-30 wt% based on the weight of gelatin powder) by electrospinning. incorporation of mca in th ... | 2008 | 21730522 |
| simultaneous quantitation of three major triterpenoid glycosides in centella asiatica extracts by high performance liquid chromatography with evaporative light scattering detection. | a high-performance liquid chromatography method with evaporative light scattering detection was established for simultaneous determination of three major triterpenoid glycosides, i.e. asiaticoside, madecassoside and asiaticoside-b, in centella asiatica extracts. the optimal chromatographic conditions were achieved on a cosmosil 5c(18)-ms-ii column by constant elution with water (0.01% trifluoroacetic acid, v/v) and acetonitrile (1.0% methyl tert-butyl ether, 0.01% trifluoroacetic acid, v/v) (78: ... | 2008 | 17703474 |
| [separation and determination of madecassic acid in triterpenic genins of centella asiatica by high performance liquid chromatography using beta-cyclodextrin as mobile phase additive]. | centella asiatica (l. ) urban is a tropical medicinal plant with a long history of therapeutic uses. madecassic acid and terminolic acid, which are a pair of structural isomers, are two constituents of centella asiatica. a method using reversed-phase high performance liquid chromatography in which beta-cyclodextrin (beta-cd) was the additive in mobile phase has been developed for separation of the structural isomers and determination of madecassic acid. the two compounds can be isolated with hig ... | 2007 | 17679419 |
| [studies on chemical constituents in herb of centella asiatica]. | to study the chemical constituents from centella asiatica. | 2007 | 17802882 |
| a randomized, double-blind, vehicle-controlled, half-side comparison with a herbal ointment containing mahonia aquifolium, viola tricolor and centella asiatica for the treatment of mild-to-moderate atopic dermatitis. | only a few clinical trials have been published on the topical treatment of atopic dermatitis with herbal ointments. an ointment containing extracts from mahonia aquifolium, viola tricolor and centella asiatica has previously been studied in open uncontrolled trials with children. however, no data exist on adult patients in a randomized controlled trial. | 2007 | 18077922 |
| comparative analysis of active constituents in centella asiatica samples from madagascar: application for ex situ conservation and clonal propagation. | a comparative quantitative analysis of the active triterpenoids in centella asiatica samples collected in different locations in madagascar was carried out to evaluate the natural variability in triterpenoid content and to select elite samples for further ex situ germplasm conservation and clonal propagation. the highest asiaticoside content (6.42%) was measured in samples collected in mangoro region. in vitro propagation of c. asiatica was successfully achieved in hormone-free medium. although ... | 2007 | 17560738 |
| [determination of aisaticoside in centella asiatica extract by hplc-elsd]. | a hplc-elsd method was established for the simultaneous quantitative determination of asiaticoside in centella asiatica extract. | 2007 | 17552155 |
| chemical components of centella asiatica and their bioactivities. | 2007 | 17498500 | |
| safety and efficacy of a ginkgo biloba-containing dietary supplement on cognitive function, quality of life, and platelet function in healthy, cognitively intact older adults. | to determine if a ginkgo biloba-containing supplement improves cognitive function and quality of life, alters primary hemostasis, and is safe in healthy, cognitively intact older adults. | 2007 | 17324660 |
| enhanced production of asiaticoside from hairy root cultures of centella asiatica (l.) urban elicited by methyl jasmonate. | transformed root ("hairy root") cultures have been shown to be a good model for the study of many secondary metabolites. however, economically important compounds such as asiaticoside and madecassoside are produced in insignificant amounts in the root of centella asiatica (l.) urban. to overcome this problem, c. asiatica was transformed using agrobacterium rhizogenes strain r1000 that harbors pcambia1302 encoding the hygromycin phosphotransferase (hpt) and green fluorescence protein (mgfp5) gene ... | 2007 | 17632725 |
| screening of indian medicinal plants for acetylcholinesterase inhibitory activity. | the cholinergic hypothesis of alzheimer's disease (ad) has provided the rationale for the current pharmaco-therapy of this disease, in an attempt to reduce the cognitive decline caused by cholinergic deficits. nevertheless, the search for potent and long-acting acetylcholinesterase (ache) inhibitors that exert minimal side effects in ad patients is still ongoing. ache inhibitors are currently the only approved therapy for the treatment of alzheimer's disease; only a limited number of drugs are c ... | 2007 | 17639556 |
| a rapid rp-hptlc densitometry method for simultaneous determination of major flavonoids in important medicinal plants. | a simple, sensitive, selective, precise, and robust high-performance tlc (hptlc) method was developed and validated for determination of flavonoids in herbal extracts bauhinia variegata, bacopa monnieri, centella asiatica, ginkgo biloba, lonicera japonica, rosa bourboniana, rosa brunonii, and rosa damascena. the hptlc of flavonoids was performed on rp-18 f(254) tlc plates with dual run, water (5% formic acid)/methanol (70:30) and water (5% formic acid)/methanol (50:50) as mobile phases. densitom ... | 2007 | 17654615 |
| centella asiatica. | 2007 | 17397269 | |
| the anti-thrombotic active constituents from centella asiatica. | the in vitro effects of a methanol extract from the aerial parts of centella asiatica on shear-induced platelet activation and coagulation were assessed after oral administration to rats, by subjecting non-anticoagulated blood to haemostatometry. 3,5-di-o-caffeoyl quinic acid, 1,5-di-o-caffeoyl quinic acid, 3,4-di-o-caffeoyl quinic acid, 4,5-di-o-caffeoyl quinic acid, and chlorogenic acid, together with asiaticoside, kaempferol, quercetine, kaempferol-3-o-beta-d-glucoside and quercetin-3-o-beta- ... | 2007 | 17473438 |
| a bioactive polyacetylene compound isolated from centella asiatica. | a polyacetylene compound was isolated from the aerial parts of centella asiatica. the chemical structure of this new compound was identified as methyl 5-[(e)-9-hydroxy-1-(1-hydroxyhexyl)-2-methoxyundeca-3,10-diene-5,7-diynyloxy]pentanoate (cadiyenol). this compound induces apoptosis (63%) independent of cell cycle regimen in mouse lymphoma cells (p388d1) at 28 microm (ic (50) = 24 +/- 2 microm) in 24 hours. the compound also reduces nitric oxide production by 70 +/- 2% in lipopolysacharride-acti ... | 2007 | 17520525 |
| beneficial effects of centella asiatica aqueous extract against arsenic-induced oxidative stress and essential metal status in rats. | the efficacy of an aqueous extract of centella asiatica (100, 200 and 500 mg/kg for 5 consecutive days) was studied in the depletion of arsenic and in the recovery of a few altered biochemical variables in arsenic pre-exposed rats (20 ppm in drinking water for 5 weeks). exposure to arsenic significantly depleted delta-aminolevulinic acid dehydratase (alad) activity, reduced glutathione (gsh) level, superoxide dismutase (sod) and increased thiobarbituric acid reactive substance (tbars) activity i ... | 2007 | 17600859 |
| adriamycin induced myocardial failure in rats: protective role of centella asiatica. | generation of reactive oxygen species and mitochondrial dysfunction has been implicated in adriamycin induced cardiotoxicity. mitochondrial dysfunction is characterized by the accumulation of oxidized lipids, proteins and dna, leading to disorganization of mitochondrial structure and systolic failure. the present study was aimed to evaluate the efficacy of centella asiatica on the mitochondrial enzymes; mitochondrial antioxidant status in adriamycin induced myocardial injury. adriamycin (2.5 mg/ ... | 2007 | 16786185 |
| chromatographic behaviour of steroidal saponins studied by high-performance liquid chromatography-mass spectrometry. | the chromatographic behaviour of steroidal saponins found in anemarrhena asphodeloides, asparagus officinalis, convallaria majalis, digitalis purpurea and ruscus aculeatus was studied by hplc-ms using a c-18 reversed-phase column and aqueous acetonitrile or aqueous methanol mobile phase gradients, with or without the addition of 1% acetic acid. the behaviour was compared to that of triterpene saponins found in aesculus hippocastanum, centella asiatica, panax notoginseng and potentilla tormentill ... | 2007 | 17391684 |
| wound-healing activity of st. john's wort (hypericum perforatum l.) on chicken embryonic fibroblasts. | wound-healing effect of st. john's wort (hypericum perforatum l.) extract was evaluated by comparing with dexpanthenol and titrated extract of centella asiatica (teca) on cultured chicken embryonic fibroblasts. chicken embryonic fibroblasts from fertilized eggs were incubated with the plant extract, dexpanthenol and teca. using microscopical methods by staining cells, mitotic ability, morphologic changes and collagen production in the cultured fibroblasts were evaluated as parameters to approach ... | 2007 | 17156955 |
| effects of traditionally used anxiolytic botanicals on enzymes of the gamma-aminobutyric acid (gaba) system. | in canada, the use of botanical natural health products (nhps) for anxiety disorders is on the rise, and a critical evaluation of their safety and efficacy is required. the purpose of this study was to determine whether commercially available botanicals directly affect the primary brain enzymes responsible for gamma-aminobutyric acid (gaba) metabolism. anxiolytic plants may interact with either glutamic acid decarboxylase (gad) or gaba transaminase (gaba-t) and ultimately influence brain gaba le ... | 2007 | 18066140 |
| radioprotective potential of plants and herbs against the effects of ionizing radiation. | ionizing radiations produce deleterious effects in the living organisms and the rapid technological advancement has increased human exposure to ionizing radiations enormously. there is a need to protect humans against such effects of ionizing radiation. attempts to protect against the deleterious effects of ionizing radiations by pharmacological intervention were made as early as 1949 and efforts are continued to search radioprotectors, which may be of great help for human application. this revi ... | 2007 | 18188408 |
| treatment with centalla asiatica (linn) fresh leaf extract enhances learning ability and memory retention power in rats. | to investigate the role of centella asiatica (cea) fresh leaf extract treatment on the behavior, especially learning and memory, of adult rats. | 2007 | 21857576 |
| repellent activity of selected plant essential oils against the malarial fever mosquito anopheles stephensi. | in recent years, use of environment friendly and biodegradable natural insecticides of plant origin have received renewed attention as agents for vector control. in this study, essential oils extracted by steam distillation from leaves of five plant species centella asiatica l., ipomoea cairica l., momordica charantia l., psidium guajava l. and tridax procumbens l. were evaluated for their topical repellency effects against malarial vector anopheles stephensi in mosquito cages. all essential oil ... | 2007 | 18209711 |
| asiaticoside induces human collagen i synthesis through tgfbeta receptor i kinase (tbetari kinase)-independent smad signaling. | skin aging appears to be principally related to a decrease in the levels of type i collagen, the primary component of the skin dermis. asiaticoside, a saponin component isolated from centella asiatica, has been shown to induce type i collagen synthesis in human dermal fibroblast cells. however, the mechanism underlying asiaticoside-induced type i collagen synthesis, especially at a molecular level, remains only partially understood. in this study, we have attempted to characterize the action mec ... | 2006 | 16557473 |
| changes in brain biogenic amines and haem biosynthesis and their response to combined administration of succimers and centella asiatica in lead poisoned rats. | this study was designed to investigate the therapeutic potential of meso 2,3-dimercaptosuccinic acid (dmsa) and one of its monoesters, monoisoamyl dmsa (miadmsa), individually or when administered in combination with an extract of centella asiatica against experimental lead intoxication in rats. biochemical variables indicative of alterations in the central nervous system and haem biosynthesis were investigated to determine the toxicity in male wistar rats. thirty five rats were exposed to 0.2% ... | 2006 | 16597373 |
| effect of centella asiatica on arsenic induced oxidative stress and metal distribution in rats. | concomitant oral supplementation of centella asiatica (100, 200 or 300 mg kg(-1), orally once daily) during arsenic exposure (20 ppm in drinking water for 4 weeks) was investigated in rats for its protective value. the animals exposed to arsenic (iii) showed a significant inhibition of delta-aminolevulinic acid dehydratase (alad) activity, a marginal decrease in glutathione (gsh) and an increase in zinc protoporphyrin (zpp) level in blood. hepatic and renal glutathione (gsh) decreased, while oxi ... | 2006 | 16389662 |
| centella asiatica (l.) leaf extract treatment during the growth spurt period enhances hippocampal ca3 neuronal dendritic arborization in rats. | centella asiatica (cea) is a creeping plant growing in damp places in india and other asian countries. the leaves of cea are used for memory enhancement in the ayurvedic system of medicine, an alternative system of medicine in india. in this study, we have investigated the effect during the rat growth spurt period of cea fresh leaf extract treatment on the dendritic morphology of hippocampal ca3 neurons, one of the regions of the brain concerned with learning and memory. neonatal rat pups (7 day ... | 2006 | 16951719 |
| evaluation of anxiolytic properties of gotukola--(centella asiatica) extracts and asiaticoside in rat behavioral models. | the ayurvedic medicinal plant gotukola (centella asiatica) was evaluated for its anxiolytic properties. specifically, this study assessed the effects of: gotukola plant materials of different genotypic origin; hexane, ethyl acetate and methanol extracts of gotukola; and asiaticoside, a triterpenic compound isolated from gotukola. various paradigms were used to assess the anxiolytic activity, including the elevated plus maze (epm), open field, social interaction, locomotor activity, punished drin ... | 2006 | 16488124 |
| a review of the microcirculation in skin in patients with chronic venous insufficiency: the problem and the evidence available for therapeutic options. | impairment of the cutaneous microcirculation is a major predisposing factor in inflammation and ulceration in patients with chronic venous insufficiency (cvi). increase of capillary filtration rate predisposes to the formation of edema. local lymphedema is a complication of cvi, often underdiagnosed. this review is focused on cvi but excludes the complication of ulceration. treatment of microcirculatory dysfunction can be done by pharmacologic intervention or compression therapy or using a combi ... | 2006 | 16928673 |
| effect of centella asiatica l (umbelliferae) on normal and dexamethasone-suppressed wound healing in wistar albino rats. | centella asiatica is a reputed medicinal plant used in the treatment of various skin diseases in the indian system of medicine. the objective of the study presented in this article was to evaluate the wound-healing potential of the ethanolic extract of the plant in both normal and dexamethasone-suppressed wound healing. the study was done on wistar albino rats using incision, excision, and dead space wounds models. the extract of c asiatica significantly increased the wound breaking strength in ... | 2006 | 16928669 |
| [plant metabolites as nootropics and cognitives]. | nowadays several millions of people suffer from alzheimer's disease and other types of dementia. etiology of these diseases is not known very well. there occur different levels of neurotransmitters, the level of acetylcholine in the brain is decreased and pathological changes affect the brain tissue. organic and toxic damage of the brain, free radicals, and other changes participate in the development of these diseases. drugs as nootropics, cognitives, and neuroprotectives are commonly used to t ... | 2006 | 17128592 |
| the effect of methyl jasmonate on triterpene and sterol metabolisms of centella asiatica, ruscus aculeatus and galphimia glauca cultured plants. | considering that exogenously applied methyl jasmonate can enhance secondary metabolite production in a variety of plant species and that 2,3-oxidosqualene is a common precursor of triterpenes and sterols in plants, we have studied centella asiatica and galphimia glauca (both synthesizing triterpenoid secondary compounds) and ruscus aculeatus (which synthesizes steroidal secondary compounds) for their growth rate and content of free sterols and respective secondary compounds, after culturing with ... | 2006 | 16876832 |
| [efficiency of includes of bioactive substances in diet of patient with hepatic encephalopathy]. | we includes 66 patients with liver cirrhosis of child-pugh b class with hepatic encephalopathy of 0 to 2nd stages in randomized interventional study. 36 patients received standard treatment and 30 patients received standard treatment + bioactive substances in formula cognoblend in capsules (2 capsules twice a day) in course of 5 weeks. formula includes extracts of plants: bacopa monneria, gingko biloba, cat's claw, gotu kola, rosemary. in group combined treatment was significant improvement of c ... | 2006 | 16729760 |
| identification of triterpenoid compounds of centella asiatica by thin-layer chromatography and mass spectrometry. | the identification of the four principal triterpenoid components of centella asiatica has been achieved by tlc on silica gel plates and mass spectrometry, as a modification of the method described in the european pharmacopoeia (5th edn). a combination of ethyl acetate and methanol as the mobile phase was found to be successful in separating these compounds from the rest of the main components of the extract. the spots were detected with anisaldehyde solution. the separated compounds were confirm ... | 2006 | 16206136 |
| a novel triterpene from centella asiatica. | a novel triterpene, 2alpha,3beta,20,23-tetrahydroxyurs-28-oic acid (1), was isolated from the aerial part of centella asiatica. its structure was elucidated by spectroscopic methods, including 2d-nmr spectra. it displayed no activity against hela and a549 cell lines at concentrations of 10 and 30 microg/ml, respectively. | 2006 | 17971739 |
| [use of a titrated extract of centella asiatica in chronic hepatic disorders (author's transl)]. | the authors report their preliminary results of a study of the possible therapeutic activity of a titrated extract of centella asiatica in chronic hepatic disorders. early results are encouraging, especially from a histological point of view, where improvement in the criteria evaluated was noted in 5 of the 12 patients treated for the longest time. these results have to be assessed in a critical manner until confirmation is obtained by complementary studies. | 2006 | 230597 |
| phlebotonics for venous insufficiency. | chronic venous insufficiency (cvi) is a common condition caused by inadequate blood flow through the veins, usually in the lower limbs. it can result in considerable discomfort with symptoms such as pain, itchiness and tiredness in the legs. sufferers may also experience swelling and ulcers. phlebotonics are a class of drugs that are often used to treat cvi. | 2005 | 16034893 |
| adjunctive periodontal treatment with centella asiatica and punica granatum extracts in supportive periodontal therapy. | centella asiatica and punica granatum are medicinal plants that have been reported to promote tissue healing and modulate host responses. preliminary study revealed positive clinical effects of an innovative preparation from the two herbal extracts in the form of biodegradable chips as a subgingival adjunct to scaling and root planing. the purpose of this research was to evaluate further augmenting efficacy the combined herbal preparation may have among maintenance patients in comparison to stan ... | 2005 | 16022023 |
| [topical chemotherapy for the treatment of burns]. | a burn is a tissue lesion which provokes different alterations that vary from topical erythema to total destruction of the structures affected. a burn always produces an alteration in the skin, a lesion in the corneal strata is sufficient to cause the skin to lose its capacity to act as a barrier. the objective the treatment for a burn has is to produce epithelization as soon as possible in order to prevent infection and to reduce functional and esthetic aftereffects. infection is the main cause ... | 2005 | 15981974 |
| cloning of a cdna probably encoding oxidosqualene cyclase associated with asiaticoside biosynthesis from centella asiatica (l.) urban. | a homology-based pcr method was used to clone a cdna encoding oxidosqualene cyclase from centella asiatica, which produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. sequence analysis of one clone found sequences related to beta-amyrin synthase. an open reading frame in the full-length clone was named cabas (centella asiatica putative beta-amyrin synthase). on the basis of amino acid sequence, cabas appears to be an enzyme (beta-amyrin synthase) that synthesi ... | 2005 | 15834571 |
| hepatotoxicity associated with the ingestion of centella asiatica. | hepatotoxicity due to herbal remedies is being increasingly recognized. centella asiatica (centella asiatica linn urban) is commercialized for multiple conditions. its active principles are pentacyclic triterpenic saponosides (asiaticoside, madecassoside). | 2005 | 15801887 |
| isobolographically additive anticonvulsant activity between centella asiatica's ethyl acetate fraction and some antiepileptic drugs. | to investigate interaction between orally given centella asiatica's ethyl acetate fraction (eaca) and intraperitoneally administered antiepileptic drugs (aeds), namely, phenytoin, valproate and gabapentin. | 2005 | 16858951 |
| in vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. | medicinal plants have many traditional claims including the treatment of ailments of infectious origin. in the evaluation of traditional claims, scientific research is important. the objective of the study was to determine the presence of antibacterial activity in the crude extracts of some of the commonly used medicinal plants in malaysia, andrographis paniculata, vitex negundo, morinda citrifolia, piper sarmentosum, and centella asiatica. in this preliminary investigation, the leaves were used ... | 2005 | 16883283 |
| precision improvement for the analysis of flavonoids in selected thai plants by capillary zone electrophoresis. | a capillary zone electrophoresis (cze) method for the analyses of kaempferol in centella asiatica and rosa hybrids and rutin in chromolaena odorata was developed. the optimization was performed on analyses of flavonoids (e.g., rutin, kaempferol, quercetin, myricetin, and apigenin) and organic carboxylic acids (e.g., ethacrynic acid and xanthene-9-carboxylic acid) by investigation of the effects of types and amounts of organic modifiers, background electrolyte concentrations, temperature, and vol ... | 2005 | 15690438 |
| cloning and expression of a farnesyl diphosphate synthase in centella asiatica (l.) urban. | a cdna encoding farnesyl diphosphate synthase (fps; ec2.5.1.1/ec2.5.1.10) was isolated from centella asiacita (l.) urban, using degenerate primers based on two highly conserved domains. a full-length cdna clone was subsequently isolated by rapid amplification of cdna ends (race) pcr. the sequence of the cafps (c. asiatica farnesyl diphosphate synthase) cdna contains an open reading frame of 1029 nucleotides encoding 343 amino acids with a molecular mass of 39.6 kda. the deduced cafps amino acid ... | 2005 | 15879717 |