| development of cost-effective media to increase the economic potential for larger-scale bioproduction of natural food additives by lactobacillus rhamnosus , debaryomyces hansenii , and aspergillus niger. | yeast extract (ye) is the most common nitrogen source in a variety of bioprocesses in spite of the high cost. therefore, the use of ye in culture media is one of the major technical hurdles to be overcome for the development of low-cost fermentation routes, making the search for alternative-cheaper nitrogen sources particularly desired. the aim of the current study is to develop cost-effective media based on corn steep liquor (csl) and locally available vinasses in order to increase the economic ... | 2009 | 19821581 |
| development of a pcr assay for the strain-specific identification of probiotic strain lactobacillus paracasei impc2.1. | recent investigations clearly indicate that the probiotic bacterium lactobacillus paracasei impc2.1 can be incorporated into vegetables to obtain innovative probiotic foods whose marketing has been authorized by the italian ministry of health. in this study, strain impc2.1 was characterized at a molecular level in order to define its taxonomic position and to develop a pcr test for strain-specific identification. molecular methods, such as 16s rrna gene sequencing and multiplex pcr, have provide ... | 2009 | 19833402 |
| comparison of in vitro models to study bacterial adhesion to the intestinal epithelium. | to evaluate the adhesion ability of intestinal bacteria to different in vitro models of intestinal epithelia, and to estimate the suitability of these models and the type of interactions involved. | 2009 | 19843211 |
| lactobacillus gg improves recovery in infants with blood in the stools and presumptive allergic colitis compared with extensively hydrolyzed formula alone. | to determine the benefits of lactobacillus rhamnosus gg (lgg) in an extensively hydrolyzed casein formula (ehcf) in improving hematochezia and fecal calprotectin over ehcf alone. | 2010 | 19880141 |
| a bifidobacterium probiotic strain and its soluble factors alleviate chloride secretion by human intestinal epithelial cells. | previous studies indicate that certain probiotic bacterial strains or their soluble products can alleviate proinflammatory cytokine secretion by intestinal epithelial cells (iec), but their impact on epithelial chloride (cl(-)) secretion remains elusive. to further decipher the mechanisms of the cross-talk between bacteria/soluble factors and epithelial cells, we analyzed the capacity of the probiotic strain bifidobacterium breve c50 (bb c50), its conditioned medium, and other commensal gram (+) ... | 2010 | 19889806 |
| lactobacillus gg in the prevention of gastrointestinal and respiratory tract infections in children who attend day care centers: a randomized, double-blind, placebo-controlled trial. | the aim of our study was to investigate the role of lactobacillus gg (lgg) in the prevention of gastrointestinal and respiratory tract infections in children who attend day care centers. | 2010 | 19896252 |
| in vitro effects of food extracts on selected probiotic and pathogenic bacteria. | a panel of 148 extracts from 37 food products was prepared using organic and aqueous solvents and both neutral and acidic conditions. the panel of food products tested included fruits, vegetables, grains, herbs and spices, most of which are common in a normal european-style diet. the impact of these extracts on the growth of selected probiotic bacteria (lactobacillus reuteri, lactobacillus rhamnosus, bifidobacteria lactis) and pathogenic bacteria (escherichia coli 0157:h7 and escherichia coli lf ... | 2009 | 19919519 |
| effect of dietary probiotics on clownfish: a molecular approach to define how lactic acid bacteria modulate development in a marine fish. | we set out to determine whether probiotic addition would improve larval development in the false percula clownfish amphiprion ocellaris and to determine what molecular responses could be observed in the larvae following probiotic exposure. we supplied the probiotic bacterial strain lactobacillus rhamnosus imc 501 to clownfish larvae from the first day posthatch simultaneously by live prey and with addition to rearing water (group 2) and exclusively by live prey (group 3). we observed twofold hig ... | 2010 | 19923354 |
| can encapsulation lengthen the shelf-life of probiotic bacteria in dry products? | this study evaluated the use of microencapsulation to maintain probiotic lactobacillus rhamnosus gg (lgg) viability during exposure to detrimentally high levels of water activity. freeze-dried lgg was mixed into a whey protein solution and sprayed onto a core particle. a second batch was prepared by applying an extra layer of palm oil onto the lgg encapsulates. viability of the lgg preparations was measured over time in an accelerated shelf-life study at 37 degrees c which included exposure to d ... | 2010 | 19948367 |
| an in vitro study on bacterial growth interactions and intestinal epithelial cell adhesion characteristics of probiotic combinations. | the aims of this study were to examine long-term growth interactions of five probiotic strains (lactobacillus casei 01, lactobacillus plantarum ha8, lactobacillus rhamnosus gg, lactobacillus reuteri atcc 55730 and bifidobacterium lactis bb12) either alone or in combination with propionibacterium jensenii 702 in a co-culture system and to determine their adhesion ability to human colon adenocarcinoma cell line caco-2. growth patterns of probiotic lactobacillus strains were not considerably affect ... | 2010 | 19949794 |
| probiotics prevent death caused by citrobacter rodentium infection in neonatal mice. | intestinal bacterial infections are a major cause of morbidity and mortality, especially in neonates. therefore, the aims of this study were to characterize citrobacter rodentium infection in neonatal mice and determine the role played by specific probiotics in ameliorating disease severity. | 2010 | 19961304 |
| combined effects of probiotic fermentation and high-pressure extraction on the antioxidant, antimicrobial, and antimutagenic activities of deodeok (codonopsis lanceolata). | this study was designed to evaluate the combined effects of probiotic fermentation and high-pressure extraction (hpe) on the functional properties of codonopsis lanceolata. the ground c. lanceolata samples were anaerobically fermented with lactobacillus acidophilus adh, bifidobacterium longum b6, lactobacillus rhamnosus gg, or lactobacillus paracasei at 37 degrees c for 10 days and subjected to 500 mpa at 50 degrees c for 30 min. the extraction yields of c. lanceolata samples were noticeably inc ... | 2010 | 20000575 |
| live and lyophilized lactobacillus species elicit differential immunomodulatory effects on immune cells. | to study the mechanism of action of the lactobacilli, splenocytes were incubated with lactobacilli. we compared the ability of live and lyophilized lactobacillus casei, lactobacillus rhamnosus gg and lactobacillus delbrueckii ssp. bulgaricus to modulate the production of interleukin 12p40 (il-12p40), tumor necrosis factor alpha and il-10 by splenocytes from c57bl/6 and balb/c mice. blocking contact between lactobacilli and immune cells abrogated all cytokine production. toll-like receptor 2 (tlr ... | 2010 | 20002187 |
| a pain in the neck: probiotics for ulcerative colitis. | | 2009 | 20008769 |
| the use of lactobacillus rhamnosus in the therapy of bacterial vaginosis. evaluation of clinical efficacy in a population of 40 women treated for 24 months. | bacterial vaginosis (bv) is the most prevalent vaginal disorder in adult women worldwide. a number of clinical studies indicate that re-establishment of the physiological ecosystem by re-colonization of vaginal mucosa by lactobacilli may be an effective therapy for bv both after initial antimicrobial therapy or when given alone. a vaginal ph <4.5 is considered physiological and its measurement has been reported as an objective parameter, very sensitive to alterations of vaginal microflora and co ... | 2010 | 20012637 |
| lactobacillus rhamnosus gg induces tumor regression in mice bearing orthotopic bladder tumors. | the present gold standard for bladder cancer is mycobacterium bovis, bacillus calmette guerin (bcg) immunotherapy. but it has a non-responder rate of 30-50% and side effects are common. lactobacillus casei strain shirota has been reported to reduce the incidence of recurrence in bladder cancer patients and to cure tumor-bearing mice. our aim was to determine if lactobacillus rhamnosus gg (lgg) could be as efficacious as bcg in a murine model of bladder cancer. mb49 bladder cancer cells secreting ... | 2010 | 20015287 |
| effect of various rinsing protocols after use of amine fluoride/stannous fluoride toothpaste on the bacterial composition of dental plaque. | this clinical study evaluated the effect of different oral hygiene protocols on the bacterial composition of dental plaque. after a 2-week period of using fluoride-free toothpaste, 30 participants followed three 1-week experimental protocols, each followed by 2-week fluoride-free washout periods in a randomized crossover examiner-blind controlled trial. the 1-week experimental protocols comprised the use of amf/snf(2) toothpaste twice daily, after which participants either (1) rinsed with tap wa ... | 2009 | 20016176 |
| lactobacillus rhamnosus gg suppresses meningitic e. coli k1 penetration across human intestinal epithelial cells in vitro and protects neonatal rats against experimental hematogenous meningitis. | the purpose of this study was to examine prophylactic efficacy of probiotics in neonatal sepsis and meningitis caused by e. coli k1. the potential inhibitory effect of lactobacillus rhamnosus gg (lgg) on meningitic e. coli k1 infection was examined by using (i) in vitro inhibition assays with e44 (a csf isolate from a newborn baby with e. coli meningitis), and (ii) the neonatal rat model of e. coli sepsis and meningitis. the in vitro studies demonstrated that lgg blocked e44 adhesion, invasion, ... | 2009 | 20016677 |
| differential induction of apoptosis in human colonic carcinoma cells (caco-2) by atopobium, and commensal, probiotic and enteropathogenic bacteria: mediation by the mitochondrial pathway. | the induction of apoptosis in mammalian cells by bacteria is well reported. this process may assist infection by pathogens whereas for non-pathogens apoptosis induction within carcinoma cells protects against colon cancer. here, apoptosis induction by a major new gut bacterium, atopobium minutum, was compared with induction by commensal (escherichia coli k-12 strains), probiotic (lactobacillus rhamnosus, bifidobacterium latis) and pathogenic (e. coli: epec and vtec) gut bacteria within the colon ... | 2010 | 20036023 |
| efficacy of whey protein gel networks as potential viability-enhancing scaffolds for cell immobilization of lactobacillus rhamnosus gg. | this study investigated cell immobilization of lactobacillus rhamnosus gg in three separate protein products: native, denatured and hydrolysed whey protein isolate (wpi). treatments were assessed for their ability to enhance probiotic survival during storage, heat stress and ex vivo gastric incubation. spatial distribution of probiotic cells within immobilized treatments was evaluated by atomic force and confocal scanning laser microscopy, while cell viability was enumerated by plate count and f ... | 2010 | 20045713 |
| lactobacilli prevent hydroxy radical production and inhibit escherichia coli and enterococcus growth in system mimicking colon fermentation. | to explore the effect of lactobacillus on redox state of colon chyme. | 2010 | 20059670 |
| probiotic attributes of lactobacillus strains isolated from food and of human origin. | lactobacilli isolated from various sources were identified on the basis of 16s-23s rrna gene intergenic region amplification and subsequent sequencing of the smaller intergenic region. an in vitro analysis of probiotic properties including binding, ability to tolerate different concentrations of bile, survival in acidic buffer and antimicrobial activity of four different isolates and two standard strains (lactobacillus plantarum american type culture collection (atcc) 8014 and l. rhamnosus gg (l ... | 2010 | 20070917 |
| analytical recovery of folate and its degradation products in human serum stored at -25 degrees c for up to 29 years. | epidemiological studies on folate and chronic diseases often involve the use of frozen serum stored in biorepositories for decades. folate instability may attenuate associations between folate status and study endpoints. in this cross-sectional study, we retrieved serum samples stored at -25 degrees c for 0, 4, 6, 17, or 29 y in the janus biobank. samples were obtained from a total of 650 men aged 40-49 y at the time of blood collection and were evenly distributed according to storage time. fola ... | 2010 | 20071651 |
| lung abscess and pleuritis caused by lactobacillus rhamnosus in an immunocompetent patient. | a 79-year-old man consulted us because of left chest pain and fever ranging from 38.0 to 38.9 °c. a chest computed tomography scan showed a mass lesion (φ40 mm) in the left lingular segment, and inflammatory markers were elevated. he was admitted with a diagnosis of lung abscess, and panipenem/betamipron was administered at a dose of 2 g/day, after which the symptoms showed slight transient resolution. however, his body temperature increased again, to more than 39.0 °c, on the eighth day of hosp ... | 2010 | 20072798 |
| production of c9,t11- and t10,c12-conjugated linoleic acids in humans by lactobacillus rhamnosus pl60. | lactobacillus rhamnosus pl60 was tested for whether it can produce c9,t11- and t10,c12-conjugated linoleic acids (clas) in human. after consumption of l. rhamnosus pl60, l. rhamnosus was detected in feces 1 week after the start of intake. analysis by gas chromatography showed that concentrations of c9,t11- and t10,c12-clas in serum had increased and concentrations of serum leptin had significantly decreased. results showed that l. rhamnosus pl60 can survive in human intestines and produce clas i ... | 2009 | 20075628 |
| safety of probiotics in patients receiving nutritional support: a systematic review of case reports, randomized controlled trials, and nonrandomized trials. | probiotics are increasingly used in patients receiving nutritional support; however, some case reports and trials have questioned their safety in such patients. | 2010 | 20089732 |
| constipation is relieved more by rye bread than wheat bread or laxatives without increased adverse gastrointestinal effects. | rye bread and lactobacilli modify the colonic environment and have the potential to relieve constipation and could be a safe and convenient alternative to laxatives. the effects of rye bread and cultured buttermilk with lactobacillus rhamnosus gg (lgg) on bowel function and colon metabolism were investigated and compared with laxatives in 51 constipated adults. they were randomized to receive whole-grain rye bread (minimum 240 g/d), lgg (2 x 10(10) colony-forming units/d), whole-grain rye bread ... | 2010 | 20089780 |
| [probiotic bacteria in the human gastrointestinal tract as a factor stimulating the immune system]. | the role of bacterial microflora of the human digestive tract in supporting the protection of the organism against food-borne pathogens and antigens is described. the effect of bacterial microflora on the immune system of the mucous surface of the intestinal tract (galt) and its effect on secretory function of the immune system, particularly regarding antibody, mainly iga, secretion, are discussed. the modulating effect of commensals and probiotics on the innate immunity response and their direc ... | 2009 | 20097952 |
| probiotics: multifarious oral vaccine against infectious traumas. | microorganisms have been used for a long time in food and alcoholic fermentation. in the last few years they have undergone scientific scrutiny of their preventative and therapeutic aspects. this has led to the discovery of a new term, probiotics. lactic acid bacteria (lab) are microbial communities normally present in the intestine of most animals. they play an important role in humans and other animals, and act as immunomodulators. they are helpful in the treatment and prevention of disease as ... | 2010 | 20100178 |
| lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection. | this study aimed to examine the potential antiviral activity of lactic acid bacteria (lab) using animal and human intestinal and macrophage cell line models of non tumor origin. to this end, lab strains selected on the basis of previous in vitro trials were co-incubated with cell line monolayers, which were subsequently challenged with rotavirus (rv) and transmissible gastroenteritis virus (tgev). in order to elucidate the possible mechanism responsible for the antiviral activity, the induction ... | 2010 | 20106541 |
| effect of stress pretreatment on survival of probiotic bacteria in gastrointestinal tract simulator. | the effect of stress pretreatment on survival of probiotic lactobacillus acidophilus la-5, lactobacillus rhamnosus gg, and lactobacillus fermentum me-3 cultures was investigated in the single bioreactor gastrointestinal tract simulator (gits). the cultures were pregrown in ph-auxostat, subjected to temperature, acid, or bile stress treatment, fast frozen in liquid nitrogen (ln(2)), and tested for survival in gits. after ln(2) freezing the colony forming ability of l. rhamnosus gg and l. fermentu ... | 2010 | 20107984 |
| inhibitory effects of lactobacillus casei subsp. rhamnosus on salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using caco-2/peripheral blood mononuclear cells. | in this study, we investigated the anti-inflammatory and reinforcing barrier effects of lactobacillus casei subsp. rhamnosus (lcr35) on caco-2 intestinal epithelial cells already exposed to salmonella lps. using the transwell co-culture model, salmonella lps was apically added to polarized caco-2 cells co-cultured with peripheral blood mononuclear cells (pbmcs) in the basolateral compartment. lps-stimulated caco-2 cells were incubated with lcr35 for 1, 6, 24 or 48 h. apical inoculation of lcr35 ... | 2010 | 20110387 |
| mucosal adhesion properties of the probiotic lactobacillus rhamnosus gg spacba and spafed pilin subunits. | lactobacillus rhamnosus gg is a well-established gram-positive probiotic strain, whose health-benefiting properties are dependent in part on prolonged residence in the gastrointestinal tract and are likely dictated by adherence to the intestinal mucosa. previously, we identified two pilus gene clusters (spacba and spafed) in the genome of this probiotic bacterium, each of which contained the predicted genes for three pilin subunits and a single sortase. we also confirmed the presence of spacba p ... | 2010 | 20118368 |
| probiotics for maintaining remission of ulcerative colitis in adults. | to evaluate evidence for probiotic efficacy for maintaining remission of ulcerative colitis (uc) in adults. | 2010 | 20124461 |
| probiotic bacteria lactobacillus rhamnosus influences the blood profile in rainbow trout oncorhynchus mykiss (walbaum). | this paper reports the effect of feeding probiotic diets on blood profiles in rainbow trout. two experiments were performed: in the first, fish of average weight 75 g were offered either a commercial feed or the same incorporated with 10(9) cfu g(-1) of lactic acid bacteria lactobacillus rhamnosus for 30 days; in the second study performed for a similar duration, fish of average weight 126 g were offered formulated diets that either contained the same bacteria in heat-killed or freeze-dried form ... | 2010 | 20127411 |
| impact of maternal probiotic-supplemented dietary counselling on pregnancy outcome and prenatal and postnatal growth: a double-blind, placebo-controlled study. | the perinatal nutritional environment impacts upon the health and well-being of mother and child also in the long term. the aim of the present study was to determine the safety and efficacy of perinatal probiotic-supplemented dietary counselling by evaluating pregnancy outcome and fetal and infant growth during the 24 months' follow-up. altogether, 256 women were randomised at their first trimester of pregnancy into a control and a dietary intervention group. the intervention group received inte ... | 2010 | 20128938 |
| anti-inflammatory actions of probiotics through activating suppressor of cytokine signaling (socs) expression and signaling in helicobacter pylori infection: a novel mechanism. | in spite of the international agency for research on cancer's definition that helicobacter pylori is the definite carcinogen of gastric cancer, the simple eradication of the bug is not enough to prevent resultant gastric cancer, and increasing microbial resistance further limits the eradication application. therefore, probiotics, non-pathogenic microbial feed that can affect the host in a beneficial manner, could be an alternate way to enhance anti-inflammation against h. pylori. however, the me ... | 2010 | 20136974 |
| lactobacillus species in supragingival plaque in subjects with hyposalivation. | the aim of the study was to analyse the frequency of different lactobacillus species in relation to the ph-lowering potential of the plaque. | 2010 | 20149344 |
| probiotic pre-treatment reduces gliclazide permeation (ex vivo) in healthy rats but increases it in diabetic rats to the level seen in untreated healthy rats. | aim: to investigate the influence of probiotic pre-treatment on the permeation of the antidiabetic drug gliclazide in healthy and diabetic rats. methods: wistar rats (age 2-3 months, weight 350 +/- 50 g) were randomly allocated into one of 4 groups (n = 16 each group): healthy control, healthy probiotic, diabetic control, and diabetic probiotic. probiotics (75 mg/kg, equal quantities of lactobacillus acidophilus, bifidobacterium lactis, and lactobacillus rhamnosus) were administered twice a day ... | 2008 | 20157366 |
| high-level expression of heme-dependent catalase gene kata from lactobacillus sakei protects lactobacillus rhamnosus from oxidative stress. | lactic acid bacteria (lab) are generally sensitive to hydrogen peroxide (h(2)o(2)), lactobacillus sakei ysi8 is one of the very few lab strains able to degrade h(2)o(2) through the action of a heme-dependent catalase. lactobacillus rhamnosus strains are very important probiotic starter cultures in meat product fermentation, but they are deficient in catalase. in this study, the effect of heterologous expression of l. sakei catalase gene kata in l. rhamnosus on its oxidative stress resistance was ... | 2010 | 20195800 |
| incidence of necrotizing enterocolitis in very-low-birth-weight infants related to the use of lactobacillus gg. | one of the five level iii neonatal intensive care units (nicu) in finland has used prophylactic lactobacillus gg (lgg) for very-low-birth-weight (vlbw) infants since 1997. | 2010 | 20219023 |
| lactobacillus by-products inhibit the growth and virulence of uropathogenic escherichia coli. | uropathogenic strains of escherichia coli (upec) can colonize the vagina and cause infections within the entire urogenital tract, including those associated with urinary tract devices. lactobacilli typically dominate the vaginal microbiota in healthy women, and studies have shown that they can inhibit upec growth and vaginal colonization. however, little is known regarding the mechanisms behind these effects. using a luciferase-based reporter construct, gradients of lactic acid, hydrogen peroxid ... | 2009 | 20224146 |
| pecorino crotonese cheese: study of bacterial population and flavour compounds. | the diversity and dynamics of the dominant bacterial population during the manufacture and the ripening of two artisanal pecorino crotonese cheeses, provided by different farms, were investigated by the combination of culture-dependent and -independent approaches. three hundred and thirty-three strains were isolated from selective culture media, clustered using restriction fragment length polymorphism and were identified by 16s rrna gene sequencing. the results indicate a decrease in biodiversit ... | 2010 | 20227601 |
| the impact of perinatal probiotic intervention on the development of overweight and obesity: follow-up study from birth to 10 years. | the achievements in combating the increasing trend of overweight and obesity have thus far been inadequate. the recently discovered instrumental role of the gut microbiota in host metabolism may offer a novel target in the prevention and management of obesity. | 2010 | 20231842 |
| probiotics and immunosenescence: cheese as a carrier. | oral intake of specific probiotics has been reported to enhance the immunity of the elderly. earlier studies have used milk or yoghurt as a probiotic carrier. we chose a commercial probiotic cheese to evaluate its potential as a probiotic food. thirty-one healthy elderly volunteers (21 female, 10 male) aged from 72 to 103 (median 86) consumed a commercial probiotic cheese containing approximately 10(9) cfu day(-1) of lactobacillus rhamnosus hn001 and lactobacillus acidophilus ncfm. the 4-week pr ... | 2010 | 20236323 |
| [establishment and evaluation of the in vitro dynamic biofilm model]. | to establish a dynamic model which could simulate the ecologic environment of oral cavity and study the cariogenicity of oral biofilm, continuously observe the formation of oral biofilm. | 2010 | 20300696 |
| probiotic colonization of the adherent mucus layer of ht29mtxe12 cells attenuates campylobacter jejuni virulence properties. | the ht29mtxe12 (e12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. in this study, we have characterized the interaction of campylobacter jejuni with the e12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate c. jejuni virulence properties. c. jejuni 81-176 colonized and reproduced in e12 mucus. adhesion to and internalization of c. jejuni were enhanced in e12 cells harb ... | 2010 | 20308300 |
| comparative evaluation of automated ribotyping and rapd-pcr for typing of lactobacillus spp. occurring in dental caries. | a group of 67 lactobacillus spp. strains containing lactobacillus casei/paracasei, lactobacillus fermentum, lactobacillus gasseri, lactobacillus plantarum, lactobacillus rhamnosus and lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (svec et al., folia microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the riboprinter microbial characterization system and by randomly amplified polymorphic d ... | 2010 | 20361257 |
| differentiation of three lactobacillus rhamnosus strains (e/n, oxy and pen) by sds-page and two-dimensional electrophoresis of surface-associated proteins. | sds-page of extracted surface-associated proteins of lactobacillus rhamnosus strains e/n, oxy, and pen, was performed. the obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used rapd genotypic method. the differentiation by the sds-page method proved to be a useful tool for strain-specific identification, which was further confirmed by 2de analysis. therefore, it can be used as an alternative or complementary method for both con ... | 2010 | 20372027 |
| porcine small intestinal epithelial cell line (ipec-j2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics. | previous studies of epithelial immune responses to rotavirus infection have been conducted in transformed cell lines. in this study, we evaluated a non-transformed porcine jejunum epithelial cell line (ipec-j2) as an in-vitro model of rotavirus infection and probiotic treatment. cell-culture-adapted porcine rotavirus (prv) osu strain, or human rotavirus (hrv) wa strain, along with lactobacillus acidophilus (la) or lactobacillus rhamnosus gg (lgg) were used to inoculate ipec-j2 cells. la or lgg t ... | 2010 | 20373994 |
| lactic acid bacteria enhance autophagic ability of mononuclear phagocytes by increasing th1 autophagy-promoting cytokine (ifn-gamma) and nitric oxide (no) levels and reducing th2 autophagy-restraining cytokines (il-4 and il-13) in response to mycobacterium tuberculosis antigen. | control of the intracellular mycobacterium tuberculosis (mtb), mainly requires an appropriate ratio of th1/th2 cytokines to induce autophagy, a physiologically, and immunologically regulated process that has recently been highlighted as an innate defense mechanism against intracellular pathogens. current vaccines/adjuvants induce both protective th1 autophagy-promoting cytokines, such as ifn-gamma, and immunosuppressive th2 autophagy-restraining cytokines, such as il-4 and il-13. tb infection it ... | 2010 | 20381647 |
| gateways to clinical trials. | (-)-epigallocatechin gallate, abafungin, ace-031, adapalene/benzoyl peroxide, ae-37, aflibercept, ags-003, albiglutide, alemtuzumab, aliskiren fumarate, alt-801, an-2728, anacetrapib, api, aprepitant, arq-197, ascorbic acid, atazanavir sulfate, atn-224, avi-4658, azacitidine, azelnidipine; belinostat, bevacizumab, bi-2536, biphasic insulin aspart, bortezomib, bovine lactoferrin, bryostatin 1, budesonide/formoterol fumarate; cac10, canfosfamide hydrochloride, cediranib, clofarabine, cocaine conju ... | 2010 | 20383346 |
| m-rtlv agar, a novel selective medium to distinguish lactobacillus casei and lactobacillus paracasei from lactobacillus rhamnosus. | we developed a novel selective medium, modified-rhamnose-2,3,5-triphenyltetrazolium chloride-lbs-vancomycin agar (m-rtlv agar), that utilizes the fermentability of l-rhamnose to distinguish lactobacillus casei and lactobacillus paracasei from lactobacillus rhamnosus. whereas l. casei and l. paracasei formed red colonies on the m-rtlv agar, l. rhamnosus formed either pink-toned colonies or white colonies with a red spot. an intervention study was conducted to confirm the capability of m-rtlv agar ... | 2010 | 20385416 |
| lactobacillus rhamnosus gg influences polyamine metabolism in hgc-27 gastric cancer cell line: a strategy toward nutritional approach to chemoprevention of gastric cance. | chemoprevention by dietary constituents has recently emerged as a novel approach to control gastric cancer incidence. over the past years, functional foods and food supplements, especially probiotics, have received much attention as potential dietary cancer prevention agents. the precise mechanisms by which these lactic cultures exert their antitumorigenic activities are not fully elucidated, but there is some evidence of their influence on cell proliferation and growth. ornithine decarboxylase ... | 2010 | 20388096 |
| administration of a synbiotic to free-living elderly and evaluation of serum cytokines. a pilot study. | ten free-living elderly were administered with a synbiotic [fermented milk containing lactobacillus rhamnosus gorbach and goldin (lgg)] and oligofructose as a prebiotic for one month. serum cytokines were evaluated before (t(0)) and after (t(1)) synbiotic administration. at t(0), values of interleukin (il)-12, il-6, il-10, il-1beta and tumor necrosis factor (tnf)-alpha were lower than normal controls, with the exception of il-8, thus confirming previous results on the impairment of both innate a ... | 2010 | 20388097 |
| improvement of l: -lactic acid production under glucose feedback controlled culture by lactobacillus rhamnosus. | in this study, a glucose feedback controller had been designed. under the controller, the glucose concentration in l: -lactic acid culture could be automatically controlled at 15 +/- 1 g/l. furthermore, l: -lactic acid production by lactobacillus rhamnosus la-04-1 in fed-batch culture was significantly increased to 170 g/l l: -lactic acid using the controller compared with 130 g/l l: -lactic acid in pulse fed-batch and 135 g/l in l: -lactic acid constant feed rate fed-batch cultures. the results ... | 2010 | 20393886 |
| immunomodulatory activity of lactobacillus rhamnosus strains isolated from goat milk: impact on intestinal and respiratory infections. | the immune stimulation induced by lactobacillus rhamnosus crl1505 (lr05) and l. rhamnosus crl1506 (lr06) on the resistance to infection with an intestinal pathogen (salmonella typhimurium) and a respiratory pathogen (streptococcus pneumoniae) was studied in swiss-albine mice experimental models. the cytokine profiles that induced the innate and specific immune response in both infectious processes were investigated. both strains were able to improve resistance against the intestinal pathogen. on ... | 2010 | 20395002 |
| lactobacillus gg in the prevention of nosocomial gastrointestinal and respiratory tract infections. | the incidence of nosocomial infections, predominantly gastrointestinal and respiratory, in children in developed countries is high, ranging from 5% to 44%. there is no effective strategy for preventing these infections. the objective of our study was to investigate the role of lactobacillus gg (lgg) in preventing nosocomial gastrointestinal and respiratory tract infections at a pediatric hospital. | 2010 | 20403940 |
| role of probiotics in the prevention of the enteric colonization by candida in preterm newborns: incidence of late-onset sepsis and neurological outcome. | to evaluate the efficacy of probiotics in the prevention of gastrointestinal colonization by candida species, of late-onset sepsis and neurological outcome in preterm newborns. | 2011 | 20410904 |
| phenotypic and genetic heterogeneity of lactic acid bacteria isolated from "alheira", a traditional fermented sausage produced in portugal. | the aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (lab) isolated from "alheira", a fermented sausage produced in portugal. lab were identified to genus and species level by phenotypic characteristics, using genus or species-specific primers and sequencing of the gene encoding 16s rrna. two-hundred and eighty-three isolates were grouped into 14 species. lactobacillus plantarum was isolated from all sausages and enterococcusfaecalis from most of ... | 2009 | 20416703 |
| living on the edge: probiotic cell surface modification and its impact on microbe-host interactions. | | 2009 | 20420219 |
| probiotics and intestinal colonization by vancomycin-resistant enterococci in mice and humans. | we investigated the impact of probiotics on the intestinal carriage of vancomycin-resistant enterococci (vre). administration of lactobacillus rhamnosus lcr35 but not escherichia coli nissle reduced, although not significantly, the density of vre colonization in a murine model. no effect of lcr35 was observed in a double-blind placebo randomized study, involving nine patients. | 2010 | 20421444 |
| [possible interaction between probiotics and warfarin]. | | 2010 | 20432867 |
| oral administration of lactobacilli from human intestinal tract protects mice against influenza virus infection. | our study was conducted to evaluate the potent protective effects of oral administration of probiotic lactobacillus strains against influenza virus (flu) infection in a mouse model. | 2010 | 20438618 |
| intranasal administration of lactobacillus rhamnosus gg protects mice from h1n1 influenza virus infection by regulating respiratory immune responses. | to investigate whether intranasal lactobacillus administration protects host animals from influenza virus (ifv) infection by enhancing respiratory immune responses in a mouse model. | 2010 | 20438620 |
| tipsitis: incidence and outcome-a single centre experience. | infection of transjugular intrahepatic portosystemic stent shunt (tipss) called 'tipsitis' has been reported but appears unusual. we report here our experience of patients who were diagnosed to have tipsitis at our centre. | 2010 | 20440117 |
| lactocin 160, a bacteriocin produced by vaginal lactobacillus rhamnosus, targets cytoplasmic membranes of the vaginal pathogen, gardnerella vaginalis. | bacterial vaginosis (bv) is a commonly occurring vaginal infection that is associated with a variety of serious risks related to the reproductive health of women. conventional antibiotic treatment for this condition is frequently ineffective because the antibiotics tend to inhibit healthy vaginal microflora along with the pathogens. lactocin 160, a bacteriocin produced by healthy vaginal lactobacilli, is a promising alternative to antibiotics; this compound specifically inhibits the bv-associate ... | 2009 | 20445810 |
| probiotic yogurt consumption is associated with an increase of cd4 count among people living with hiv/aids. | to evaluate the long term effect of yogurt supplemented with lactobacillus rhamnosus fiti on the immune function (cd4 count) of people living with hiv/aids. | 2010 | 20463586 |
| oral lactoferrin for the prevention of sepsis and necrotizing enterocolitis in preterm infants. | lactoferrin, a normal component of human colostrum, milk, tears and saliva can enhance host defence and may be effective in the prevention of sepsis and necrotizing enterocolitis (nec) in preterm neonates. | 2010 | 20464748 |
| characterization of maba, a modulator of lactobacillus rhamnosus gg adhesion and biofilm formation. | abstract the probiotic lactobacillus rhamnosus gg, first isolated from healthy human gut microbiota, has been reported to adhere very well to components of the intestinal mucosa, thereby enabling transient colonization of the gastrointestinal tract (git). in a search for the genes responsible for the good adherence capacity of this strain, a genomic region encoding a protein with homology to putative adhesion proteins (lgg_01865) and its putative regulator (lgg_01866) was identified. the sequenc ... | 2010 | 20482623 |
| enhancing the biotransformation of isoflavones in soymilk supplemented with lactose using probiotic bacteria during extended fermentation. | soymilk (sm) lacks lactose; hence supplementation of sm with lactose is likely to enhance the growth of probiotic bacteria and biotransformation of isoflavone glycosides to isoflavone aglycones. in this study, 11 strains of probiotic bacteria including lactobacillus rhamnosus, l. salivarius, l. plantarum, l. acidophilus, l. paracasei, howaru l. rhamnosus, l. delbrueckii subsp. bulgaricus, bifidobacterium lactis type bi-07, b. longum, howaru b. bifidum, and b lactis type bi-04 were inoculated ind ... | 2010 | 20492303 |
| probiotics in the critically ill patient: a double blind, randomized, placebo-controlled trial. | probiotics have been shown to be able to restore a non-pathogenic digestive flora, to prevent digestive colonization by pathogenic bacteria, and to modulate immunity. the aim of this study was to assess the effects of prophylactic probiotic administration in patients ventilated for up to 2 days. | 2010 | 20502866 |
| probiotic prophylaxis of ventilator-associated pneumonia: a blinded, randomized, controlled trial. | enteral administration of probiotics may modify the gastrointestinal environment in a manner that preferentially favors the growth of minimally virulent species. it is unknown whether probiotic modification of the upper aerodigestive flora can reduce nosocomial infections. | 2010 | 20522788 |
| capsular and slime-polysaccharide production by lactobacillus rhamnosus jaas8 isolated from chinese sauerkraut: potential application in fermented milk products. | exopolysaccharides (epss) produced by lactic acid bacteria (lab) play an important role in the improvement of the physical properties of fermented dairy products. to find eps-producing lab strains with potential industrial applications, a lactobacillus rhamnosus strain, l. rhamnosus jaas8, that is capable of producing two forms of eps when grown in mrs broth or semi-defined medium with glucose as a carbon source was isolated and identified from chinese sauerkraut. the capsular-polysaccharide (cp ... | 2010 | 20541116 |
| probiotics in pregnant women to prevent allergic disease: a randomized, double-blind trial. | previous reports have suggested that certain probiotics given to mothers and children at risk of atopy halves the incidence of atopic dermatitis (ad) at 2 years of age. | 2010 | 20545688 |
| fecal bacteriotherapy for relapsing clostridium difficile infection in a child: a proposed treatment protocol. | clostridium difficile infection (cdi) is a potentially serious emerging infectious disease. the incidences of cdi in childhood and cdi cases complicated by relapses have increased by 50% or more in north america during the past 2 decades. we report here the case of a 2-year-old child with relapsing cdi caused by the epidemic strain bi/nap1/o27 that was refractory to saccharomyces boulardii and lactobacillus rhamnosus gg probiotics and to intensive therapy with traditional (metronidazole, vancomy ... | 2010 | 20547640 |
| cloning, expression, purification, and activity assay of proteins related to d-lactic acid formation in lactobacillus rhamnosus. | two proteins that might be responsible for d-lactic acid (d-la) formation were screened from the genome database of lactobacillus rhamnosus gg. the coding genes of the two proteins in l. rhamnosus casl, ldhd1 and ldhd2, were cloned and expressed in escherichia coli rosetta with an inducible expression vector petduet-1 (novagen, darmstadt, germany), respectively. the two purified proteins, ldhd-1 and ldhd-2, migrated as a single protein band separately, both corresponding to an apparent molecular ... | 2010 | 20549201 |
| immune-stimulating effects of lactic acid bacteria in vivo and in vitro. | the health-promoting effects of lactic acid bacteria (lab) are well recognised, making them a popular functional food ingredient. commercially available probiotic products are often promoted as capable of improving immune defences also in healthy subjects. however, while strain-specific differences exist in the effects of lab, conventional yoghurt bacteria have proved beneficial as well. for comparing the immunological effects of conventional and probiotic lab, young healthy women received eithe ... | 2010 | 20550748 |
| improvement of l(+)-lactic acid production from cassava wastewater by lactobacillus rhamnosus b 103. | l(+)-lactic acid is used in the pharmaceutical, textile and food industries as well as in the synthesis of biodegradable plastics. the aim of this study was to investigate the effects of different medium components added in cassava wastewater for the production of l(+)-lactic acid by lactobacillus rhamnosus b 103. | 2010 | 20564419 |
| long-term vaginal administration of lactobacillus rhamnosus as a complementary approach to management of bacterial vaginosis. | to investigate the advantages of long-term vaginal administration of lactobacillus rhamnosus after oral treatment with metronidazole to prevent the recurrence of bacterial vaginosis (bv). | 2010 | 20573348 |
| randomized controlled trial of probiotics for the prevention of spontaneous preterm delivery associated with intrauterine infection: study protocol. | abstract: background: spontaneous preterm deliveries that occur before the 34th week of gestation, and particularly before the 32nd week of gestation, have been strongly associated to intrauterine infection, ascending from vagina, and represent the largest portion of neonatal deaths and neurological problems. bacterial vaginosis, characterized by a diminished or absent flora of lactobacilli and increased colonization of several anaerobic or facultative microorganisms, increases two times the ris ... | 2010 | 20591191 |
| the effects of lactobacillus rhamnosus on the prevention of asthma in a murine model. | lactobacilli are probiotic bacteria that are effective in the management of allergic diseases or gastroenteritis. it is hypothesized that such probiotics have immunoregulatory properties and promote mucosal tolerance. our goal was to investigate whether lactobacillus casei rhamnosus lcr35 could inhibit airway inflammation in an ovalbumin (ova)-induced murine model of asthma. | 2010 | 20592920 |
| evaluation of antimicrobial activity of probiotic bacteria against salmonella enterica subsp. enterica serovar typhimurium 1344 in a common medium under different environmental conditions. | the importance of probiotics in human nutrition has been gaining recognition in recent years. these organisms have been shown to promote human health by enhancing immunological and digestive functions and fighting respiratory tract infections. we propose an improved in vitro model for the study of probiotic antimicrobial activity against enteropathogens, by attempting to re-create, in a common culture medium, environmental growth conditions comparable to those present in the small intestine. a p ... | 2010 | 20600855 |
| inhibition of shigella sonnei adherence to ht-29 cells by lactobacilli from chinese fermented food and preliminary characterization of s-layer protein involvement. | in this study, seven lactobacilli with a high degree of antagonistic activity against three pathogens and good adherence to ht-29 cells were selected. the ability of these seven lactobacilli to inhibit adhesion of shigella sonnei to intestinal mucosa was studied on cultured ht-29 cells. lactobacilli were added simultaneously with, before or after s. sonnei to test for their effectiveness in exclusion, competition and displacement assays, respectively. lactobacillus paracasei subp. paracasei m5-l ... | 2010 | 20600857 |
| efficacy of probiotic lactobacillus gg on allergic sensitization and asthma in infants at risk. | probiotics are perceived to exert beneficial effects in the prevention and treatment of allergic diseases. | 2010 | 20604800 |
| lactobacillus plantarum dsm 2648 is a potential probiotic that enhances intestinal barrier function. | the aim of this research was to identify bacterial isolates having the potential to improve intestinal barrier function. lactobacillus plantarum strains and human oral isolates were screened for their ability to enhance tight junction integrity as measured by the transepithelial electrical resistance (teer) assay. eight commercially used probiotics were compared to determine which had the greatest positive effect on teer, and the best-performing probiotic strain, lactobacillus rhamnosus hn001, w ... | 2010 | 20618863 |
| adhesive properties, extracellular protein production, and metabolism in the lactobacillus rhamnosus gg strain when grown in the presence of mucin. | this paper examines the probiotic bacterium lactobacillus rhamnosus gg, and how it reacts to the presence of mucin in its extracellular milieu. parameters studied included cell clustering, adhesion to mucin, extracellular protein production, and formation of final metabolites. l. rhamnosus gg was found to grow efficiently in the presence of glucose, n-acetylglucosamine, or mucin (partially purified or purified) as sole carbon sources. however, it was unable to grow using other mucin constituents ... | 2010 | 20622495 |
| efficient production of l-lactic acid from cassava powder by lactobacillus rhamnosus. | cassava is one of the most efficient and rich crops in terms of carbohydrate production, which is a tropical perennial plant that grows on poor or depleted soils. microbial conversion of such a renewable raw material to useful products is an important objective in industrial biotechnology. l-lactic acid was efficiently produced from cassava powder by a lactobacillus rhamnosus strain casl. the fermentation properties of cassava powder were compared with those of glucose and corn powder. the effic ... | 2010 | 20627717 |
| improving the stability of probiotic bacteria in model fruit juices using vitamins and antioxidants. | this study examined the survival of probiotic bacteria in a model fruit juice system. three different strains of probiotic bacteria were used in this study: howaru lactobacillus rhamnosus hn001, howaru bifidobacterium lactis hn001, and lactobacillus paracasei lpc 37. the probiotic bacteria were inoculated into model juice with various vitamins and antioxidants, namely white grape seed extract, green tea extract, vitamin b2, vitamin b3, vitamin b6, vitamin c, and vitamin e. the model juice withou ... | 2010 | 20629884 |
| screening and selection of stress resistant lactobacillus spp. isolated from the marine oyster (crassostrea gigas). | we attempted to isolate lactobacillus spp. from the marine oyster (crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. a total of 83 lactobacilli strains were isolated from oyster. they were all gram-positive, rod-shaped and catalase-negative. by performing a stress resistance assay, we selected eighteen isolates. based on 16s rrna gene sequencing, lactobacillus paracasei was the most prevalent species among the selected isolates. ... | 2010 | 20633689 |
| use of viability staining in combination with flow cytometry for rapid viability assessment of lactobacillus rhamnosus gg in complex protein matrices. | the aim of this study was to demonstrate that flow cytometry (facs) could potentially be employed for rapid viability assessment of probiotic bacteria immobilized or encapsulated in complex matrices. lactobacillus rhamnosus gg was immobilized within six different protein environments using whey protein isolate (wpi) and yoghurt matrices and encapsulated within protein micro-beads, all of which ranged in structural complexity. following a series of environmental-stress trials, survival of the str ... | 2010 | 20638420 |
| lactobacillus rhamnosus gg attenuates interferon-{gamma} and tumour necrosis factor-alpha-induced barrier dysfunction and pro-inflammatory signalling. | the intestinal epithelium forms a protective barrier against luminal contents and the external environment, mediated via intercellular tight junctions (tjs). the tj can be disrupted via cell signalling induced by either enteric pathogens or pro-inflammatory cytokines, thereby contributing to various intestinal disorders ranging from acute infectious diarrhoea to chronic inflammatory bowel diseases. probiotics, such as lactobacillus rhamnosus gg (lgg), are reported to confer beneficial effects on ... | 2010 | 20656777 |
| efficacy of vaginal probiotic capsules for recurrent bacterial vaginosis: a double-blind, randomized, placebo-controlled study. | we assessed the effectiveness of vaginal probiotic capsules for recurrent bacterial vaginosis (bv) prevention. | 2010 | 20659602 |
| lactobacillus rhamnosus cell lysate in the management of resistant childhood atopic eczema. | the number of children suffering from atopic eczema has increased over the past 30 years especially in children between the ages of 2 and 5 years. these is a significant group of eczematous children that are resistant to standard therapy. babies and children with eczema suffer pain, irritation and disfigurement from the dermatitis. in this study, we have followed 14 cases of pediatric patients (ages of 8 months to 64 months) with a history of resistant eczema for a period of at least six months. ... | 2010 | 20687891 |
| tolerance and safety of the potentially probiotic strain lactobacillus rhamnosus prsf-l477: a randomised, double-blind placebo-controlled trial in healthy volunteers. | in europe, the species lactobacillus rhamnosus is currently on the qualified presumption of safety list used by the european food safety authority (efsa) for internal safety assessment, but according to the efsa the species should remain a topic of surveillance. in the present study, the safety and tolerance of the potentially probiotic strain l. rhamnosus prsf-l477 was investigated in a placebo-controlled double-blind volunteer trial following fao/who guidelines. a total of thirty-four subjects ... | 2010 | 20691131 |
| heat-killed lactobacillus rhamnosus gg modulates urocortin and cytokine release in primary trophoblast cells. | a number of studies are showing that probiotic treatment induces an anti-inflammatory state. intrauterine infection can lead to preterm delivery by modulating immune function and efforts to prevent this condition are ongoing nowadays. lactobacillus rhamnosus gg (lgg) is a probiotic known to ameliorate inflammation by increasing local anti-inflammatory mediators in urinary and gastrointestinal tracts. the present study then analyzed the effect of heat-killed lgg over β-hcg, progesterone, interleu ... | 2010 | 20696472 |
| the dnak gene as a molecular marker for the classification and discrimination of the lactobacillus casei group. | it is hard to accurately identify specific species of the lactobacillus casei group using phenotypic techniques alone. some strains of this species group are considered to be probiotic and are widely applied in the food industry. in this study, we compared the use of two phylogenetic markers, the 16s rrna and dnak genes, for species discrimination of the members of the l. casei group using sequencing and rflp. the results showed that l. casei, lactobacillus paracasei, lactobacillus zeae and lact ... | 2011 | 20700765 |
| the plackett-burman design in optimization of media components for biomass production of lactobacillus rhamnosus oxy. | the central composite design was developed to search for an optimal medium for the growth of lactobacillus rhamnosus oxy. the effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins b, amino acids) was examined. the first-order model based on plackett-burman design showed that glucose, sodium pyruvate, meat extract and mineral salts significantly influenced the growth of the examined bacteria. the second-order ... | 2010 | 20724280 |
| impact of lipoteichoic acid modification on the performance of the probiotic lactobacillus rhamnosus gg in experimental colitis. | while some probiotic strains might have adjuvant effects in the therapy for inflammatory bowel diseases (ibd), these effects remain controversial and cannot be generalized. in this study, a dltd mutant of the model probiotic lactobacillus rhamnosus gg (lgg), having a drastic modification in its lipoteichoic acid (lta) molecules, was analysed for its effects in an experimental colitis model. dextran sulphate sodium (dss) was used to induce either moderate to severe or mild chronic colitis in mice ... | 2010 | 20731672 |
| evaluation of quantitative pcr measurement of bacterial colonization of epithelial cells. | microbial colonization is an important step in establishing pathogenic or probiotic relations to host cells and in biofilm formation on industrial or medical devices. the aim of this work was to verify the applicability of quantitative pcr (real-time pcr) to measure bacterial colonization of epithelial cells. salmonella enterica and caco-2 intestinal epithelial cell line was used as a model. to verify sensitivity of the assay a competition of the pathogen cells to probiotic microorganism was tes ... | 2010 | 20734752 |