| subtractive screening for probiotic properties of lactobacillus species from the human gastrointestinal tract in the search for new probiotics. | in the search for new probiotics, 61 lactobacillus spp. isolates, belonging to 12 species and isolated as dominant lactic acid bacteria from the feces of healthy humans, were subjected to a subtractive system of in vitro analyses, which included desirable and undesirable traits. twenty-four isolates were able to grow in 2% bovine bile, of which 13 grew in acidified broth at ph 3.5 in acidified cysteine-containing mrs broth. intrinsic resistance to certain antimicrobial agents (cefoxitin, metroni ... | 2007 | 17995611 |
| single laboratory method performance evaluation for the analysis of total food folate by trienzyme extraction and microplate assay. | single laboratory method performance parameters, including the calibration curve, accuracy, recovery, precision, limit of detection (lod), and limit of quantification (loq), were evaluated for the analysis of total food folate by the trienzyme extraction and microplate assay with lactobacillus casei subsp. rhamnosus. standard reference material (srm) 1546 (meat homogenate), srm 2383 (baby food composite), srm 1846 (infant formula), certified reference material (crm) 121 (wholemeal flour), and cr ... | 2007 | 17995709 |
| survival in food systems of lactobacillus rhamnosus r011 microentrapped in whey protein gel particles. | the aim of this study was to investigate the effect of whey protein isolate (wpi) gel microentrapment on the viability of lactobacillus rhamnosus r011 during the production and storage of biscuits, frozen cranberry juice, and vegetable juice. viability of microentrapped (me) cells was compared to free cells freeze-dried in a milk-based protective solution as well as in a wpi-based solution (ungelled). during the production of biscuits and their storage for 2 wk at 23 degrees c, the highest stabi ... | 2007 | 17995889 |
| mechanisms of probiotic-host interaction with ibd as a case study: a role for exopolysaccharides? | | 2007 | 18018857 |
| enhancement of functional characteristics of mixed lactic culture producing nisin z and exopolysaccharides during continuous prefermentation of milk with immobilized cells. | antagonistic phenomena between strains often occur in mixed cultures containing a bacteriocinogenic strain. a nisin z producer (lactococcus lactis ssp. lactis biovar. diacetylactis ul719) and 2 nisin-sensitive strains for acidification (lactococcus lactis ssp. cremoris atcc19257) and exopolysaccharide (eps) production (lactobacillus rhamnosus rw-9595m) were immobilized separately in gel beads and used to continuously preferment milk at different temperatures, with ph controlled at 6.0 by fresh m ... | 2007 | 18024726 |
| combining strains of lactic acid bacteria may reduce their toxin and heavy metal removal efficiency from aqueous solution. | the primary objective of this study was to compare the removal of cadmium, lead, aflatoxin b1 and microcystin-lr from aqueous solution by lactobacillus rhamnosus gg, l. rhamnosus lc705, propionibacterium freudenreichii shermanii js and bifidobacterium breve bbi99/e8, separately and in combination. | 2008 | 18028332 |
| acid, bile, and heat tolerance of free and microencapsulated probiotic bacteria. | eight strains of probiotic bacteria, including lactobacillus rhamnosus, bifidobacterium longum, l. salivarius, l. plantarum, l. acidophilus, l. paracasei, b. lactis type bl-o4, and b. lactis type bi-07, were studied for their acid, bile, and heat tolerance. microencapsulation in alginate matrix was used to enhance survival of the bacteria in acid and bile as well as a brief exposure to heat. free probiotic organisms were used as a control. the acid tolerance of probiotic organisms was tested usi ... | 2007 | 18034741 |
| rapid identification of lactobacillus and bifidobacterium in probiotic products using multiplex pcr. | lactic acid bacteria (lab) are beneficial for the gastrointestinal tract and reinforce immunity in human health. recently, many functional products using the lactic acid bacteria have been developed. among these lab, lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium longum, and bifidobacterium bifidum are frequently used for probiotic products. in order to monitor these lab in commercial probiotic products, a multiplex pcr method was developed. we designed four species-specific ... | 2007 | 18050954 |
| direct selection and phage display of a gram-positive secretome. | surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. however, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. we have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins. | 2007 | 18078523 |
| [two cases of lactobacillus rhamnosus infection and pancreatitis]. | lactobacilli rhamnosus infections are rare and occur in patients with severe conditions causing immunosuppression and/or in those who have received intense antibiotic treatment such as with teicoplanin and vancomycin. we report two cases which occurred within a period of 48 hours, during regression of pancreatitis in two women aged 35 and 70 years old, hospitalized in the same department. several criteria pointed towards two independent infections but the rarity and chronology of the appearance ... | 2008 | 18096340 |
| intestinal permeability and effects of lactobacillus rhamnosus therapy in children with short bowel syndrome. | the present study was conducted to investigate the effects of lactobacillus rhamnosus (also known as lgg) on intestinal permeability (ip) in children with short bowel syndrome (sbs). | 2008 | 18162832 |
| clinical indications for probiotics: an overview. | probiotic bacteria are used to treat or prevent a broad range of human diseases, conditions, and syndromes. in addition, there are areas of medical use that have been proposed for future probiotic applications. randomized double-blind studies have provided evidence of probiotic effectiveness for the treatment and prevention of acute diarrhea and antibiotic-induced diarrhea, as well as for the prevention of cow milk-induced food allergy in infants and young children. research studies have also pr ... | 2008 | 18181732 |
| use of lactobacillus casei subspecies rhamnosus gg and gastrointestinal colonization by candida species in preterm neonates. | candida species increasingly cause morbidity and mortality in the premature infant in neonatal intensive care units, and the gut reservoir is the site from which dissemination most frequently starts in such patients. specific antifungal prophylaxis is the most suitable strategy with which to limit the severity and the frequent neurodevelopmental impairment in survivors that is associated with neonatal invasive fungal infections. recent interest has focused on the use of probiotics for the treatm ... | 2007 | 18185091 |
| microencapsulation of a recombinant aminopeptidase (pepn) from lactobacillus rhamnosus s93 in chitosan-coated alginate beads. | a recombinant aminopeptidase (90 kda) of lactobacillus rhamnosus s93 produced by e. coli was encapsulated in alginate or chitosan-treated alginate beads prepared by an extrusion method. this study investigated the effects of alginate, cacl2, chitosan concentrations, hardening time, ph and alginate/enzyme ratios on the encapsulation efficiency (ee) and the enzyme release (er). chitosan in the gelling solution significantly increased the ee from 30.2% (control) to 88.6% (coated). this polycationic ... | 2008 | 18188731 |
| probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model. | the transgenomic metabolic effects of exposure to either lactobacillus paracasei or lactobacillus rhamnosus probiotics have been measured and mapped in humanized extended genome mice (germ-free mice colonized with human baby flora). statistical analysis of the compartmental fluctuations in diverse metabolic compartments, including biofluids, tissue and cecal short-chain fatty acids (scfas) in relation to microbial population modulation generated a novel top-down systems biology view of the host ... | 2008 | 18197175 |
| meta-analysis of clinical trials of probiotics for prevention and treatment of pediatric atopic dermatitis. | prenatal and postnatal probiotic supplementation for prevention and treatment of pediatric atopic dermatitis (pad) has been studied in clinical trials, but results have been mixed and hindered by heterogeneity of study design. | 2008 | 18206506 |
| fecal microflora of greek healthy neonates. | this study aimed to explore, in our geographical region, the development of intestinal microflora and the colonization patterns of lactic acid bacteria and bifidobacteria during the first three months of life and to investigate the effect of the mode of delivery. fecal specimens from 82 healthy, full-term infants were collected prospectively 4, 30 and 90 days after delivery and subcultured on nonselective and selective media. identification of isolates was performed by microbiological and molecu ... | 2008 | 18207437 |
| expression of chemokine/cytokine genes and immune cell recruitment following the instillation of mycobacterium bovis, bacillus calmette-guérin or lactobacillus rhamnosus strain gg in the healthy murine bladder. | mycobacterium bovis, bacillus calmette-guérin (bcg) is the current gold standard for bladder cancer therapy. in this study a profile of the gene expression changes that occur after bcg instillation in the bladders of healthy mice was produced and compared to the type of immune cells recruited into the bladder. a similar comparison was made for lactobacillus rhamnosus strain gg (lgg) instillations in healthy mice to determine its potential in the immunotherapy of bladder cancer. mice were given s ... | 2008 | 18217952 |
| yogurt containing probiotic lactobacillus rhamnosus gr-1 and l. reuteri rc-14 helps resolve moderate diarrhea and increases cd4 count in hiv/aids patients. | hiv/aids is changing the human landscape in sub-saharan africa. relatively few patients receive antiretroviral therapy, and many suffer from debilitating diarrhea that affects their quality of life. given the track record of probiotics to alleviate diarrhea, conventional yogurt fermented with lactobacillus delbruekii var bulgaricus and streptococcus thermophilus was supplemented with probiotic lactobacillus rhamnosus gr-1 and l. reuteri rc-14. twenty-four hiv/aids adult female patients (18 to 44 ... | 2008 | 18223503 |
| lactobacillus rhamnosus strain gg prevents enterohemorrhagic escherichia coli o157:h7-induced changes in epithelial barrier function. | enterohemorrhagic escherichia coli (ehec) o157:h7 intimately attaches to intestinal epithelial monolayers and produces attaching and effacing (a/e) lesions. in addition, ehec infection causes disruptions of intercellular tight junctions, leading to clinical sequelae that include acute diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome. current therapy remains supportive since antibiotic therapy increases the risk of systemic complications. this study focused on the potential therap ... | 2008 | 18227169 |
| state transitions and physicochemical aspects of cryoprotection and stabilization in freeze-drying of lactobacillus rhamnosus gg (lgg). | the frozen and dehydrated state transitions of lactose and trehalose were determined and studied as factors affecting the stability of probiotic bacteria to understand physicochemical aspects of protection against freezing and dehydration of probiotic cultures. | 2008 | 18248378 |
| immunostimulatory oligodeoxynucleotide containing tttcgttt motif from lactobacillus rhamnosus gg dna potentially suppresses ova-specific ige production in mice. | in a previous study of the immunoregulatory properties of commensal bacterial dna, we identified the strong immunostimulatory oligodeoxynucleotide (iss-odn) id35 in the genomic dna of lactobacillus rhamnosus gg (lgg). the observed effects of id35 are because of the unique tttcgttt motif located at the 5' end of the odn, which is different from the previously identified iss motifs in humans and mice. in the present study, we used an ovalbumin (ova)-sensitized mouse model to show that id35 is a po ... | 2008 | 18248528 |
| probiotics for treatment of clostridium difficile-associated colitis in adults. | probiotics are live microorganisms consisting of non-pathogenic yeast and bacteria that are believed to restore the microbial balance of the gastrointestinal tract altered by infection with clostridium difficile (c. difficile). | 2008 | 18254055 |
| safety study of an antimicrobial peptide lactocin 160, produced by the vaginal lactobacillus rhamnosus. | to evaluate the safety of the antimicrobial peptide, lactocin 160. | 2007 | 18273406 |
| characterization of oral lactobacilli as potential probiotics for oral health. | intestinal lactobacilli have been successfully used as probiotics to treat gastrointestinal disorders, but only limited data are available for the probiotic properties of oral lactobacilli to combat oral diseases. we aimed to characterize oral lactobacilli for their potential probiotic properties according to the international guidelines for the evaluation of probiotics, and to select potential probiotic strains for oral health. | 2008 | 18279182 |
| effect of wild strains used as starter cultures and adjunct cultures on the volatile compounds of the pecorino siciliano cheese. | the effect of six wild strains on the volatile profile of the ps cheese was investigated and compared to that generated from industrial starters generally used to produce ps cheese. all cheeses were subjected to microbiological, physicochemical, and volatile compounds analyses. the dgge of the 16s rdna analysis was also applied. the volatile compounds generated during ripening were studied through the spme and the gc-ms methods. no difference was detected between the experimental and control che ... | 2008 | 18281116 |
| probiotics for the treatment or prevention of atopic dermatitis: a review of the evidence from randomized controlled trials. | probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. to synthesize the evidence for the effectiveness of probiotics in the treatment or prevention of atopic dermatitis (ad) in children, we reviewed the results of 13 relevant randomized (placebo)-controlled trials (rcts), 10 of which evaluated probiotics as treatment and 3 for prevention of ad. the main outcome measure in 9 rcts was the change in scorad (scoring atopic de ... | 2008 | 18284263 |
| genome shuffling enhanced l-lactic acid production by improving glucose tolerance of lactobacillus rhamnosus. | genome shuffling is a powerful strategy for rapid engineering of microbial strains for desirable industrial phenotypes. here we applied the genome shuffling to improve the glucose tolerance of lactobacillus rhamnosus atcc 11443 while simultaneously enhancing the l-lactic acid production. the starting population was generated by ultraviolet irradiation and nitrosoguanidine mutagenesis and then subjected for the recursive protoplast fusion. the positive colonies from library created by fusing the ... | 2008 | 18289712 |
| probiotics ameliorate the hydrogen peroxide-induced epithelial barrier disruption by a pkc- and map kinase-dependent mechanism. | probiotics promote intestinal epithelial integrity and reduce infection and diarrhea. we evaluated the effect of lactobacillus rhamnosus gg-produced soluble proteins (p40 and p75) on the hydrogen peroxide-induced disruption of tight junctions and barrier function in caco-2 cell monolayers. pretreatment of cell monolayers with p40 or p75 attenuated the hydrogen peroxide-induced decrease in transepithelial resistance and increase in inulin permeability in a time- and dose-dependent manner. p40 and ... | 2008 | 18292183 |
| probiotics: sorting the evidence from the myths. | probiotics consist of yeast or bacteria, especially lactic acid bacteria. they are available as capsules, powder, fermented milks or yoghurts. probiotics exhibit strain-specific differences in their resistance to acid and bile, ability to colonise the gastrointestinal tract, clinical efficacy, and benefits to the health of the host. there is level i evidence for the use of probiotics in treating acute infectious diarrhoea and preventing antibiotic-associated diarrhoea, with lactobacillus rhamnos ... | 2008 | 18312197 |
| taxonomic and strain-specific identification of the probiotic strain lactobacillus rhamnosus 35 within the lactobacillus casei group. | lactobacilli are lactic acid bacteria that are widespread in the environment, including the human diet and gastrointestinal tract. some lactobacillus strains are regarded as probiotics because they exhibit beneficial health effects on their host. in this study, the long-used probiotic strain lactobacillus rhamnosus 35 was characterized at a molecular level and compared with seven reference strains from the lactobacillus casei group. analysis of rrn operon sequences confirmed that l. rhamnosus 35 ... | 2008 | 18326671 |
| lactobacillus rhamnosus strain gg restores alkaline phosphatase activity in differentiating caco-2 cells dosed with the potent mycotoxin deoxynivalenol. | deoxynivalenol (don) contamination of cereal crops occurs frequently, and may cause acute exposure at high levels or chronic more moderate exposure. don has proven toxicity including restriction of enterocyte differentiation, which may play a part in don induced gastroenteritis. the probiotic bacteria lactobacillus rhamnosus strain gg (gg) can bind don, and therefore potentially restrict bioavailability of this toxin. binding efficacy is not significantly altered by heat treatment, and therefore ... | 2008 | 18343010 |
| "swiss cheese-like" brain due to lactobacillus rhamnosus. | | 2008 | 18347323 |
| characterization and site-directed mutagenesis of wzb, an o-phosphatase from lactobacillus rhamnosus. | reversible phosphorylation events within a polymerisation complex have been proposed to modulate capsular polysaccharide synthesis in streptococcus pneumoniae. similar phosphatase and kinase genes are present in the exopolysaccharide (eps) biosynthesis loci of numerous lactic acid bacteria genomes. | 2008 | 18387182 |
| diversity and dynamics of lactobacilli populations during ripening of rdo camembert cheese. | the diversity and dynamics of lactobacillus populations in traditional raw milk camembert cheese were monitored throughout the manufacturing process in 3 dairies. culture-dependent analysis was carried out on isolates grown on acidified de man - rogosa - sharpe agar and lactobacillus anaerobic de man rogosa sharpe agar supplemented with vancomycin and bromocresol green media. the isolates were identified by polymerase chain reaction - temperature gradient gel electrophoresis (pcr-tgge) and (or) ... | 2008 | 18388993 |
| [lactobacillus rhamnosus septicemia in a diabetic patient associated with probiotic use: a case report]. | probiotic agents are increasingly used as over the counter drugs for the treatment of a variety of inflammatory and infectious conditions. the rationale of their use seems to restore a friendly bacterial flora in the gut. complications of probiotic use, although rarely reported, can occur. | 2008 | 18390430 |
| probiotic intervention has strain-specific anti-inflammatory effects in healthy adults. | to evaluate the effects of three potentially anti-inflammatory probiotic bacteria from three different genera on immune variables in healthy adults in a clinical setting based on previous in vitro characterization of cytokine responses. | 2008 | 18395902 |
| clinical trial: effectiveness of lactobacillus rhamnosus (strains e/n, oxy and pen) in the prevention of antibiotic-associated diarrhoea in children. | convincing evidence that probiotic administration can lower the risk of antibiotic-associated diarrhoea is limited to certain micro-organisms. | 2008 | 18410562 |
| effect of lactobacillus rhamnosus gg on rbet v1 and rmal d1 specific iga in the saliva of patients with birch pollen allergy. | lactobacillus rhamnosus gg (lgg) has demonstrated promising results in the treatment and prevention of atopic eczema. | 2008 | 18450119 |
| the potential influence of fruit polyphenols on colonic microflora and human gut health. | the effect of common dietary polyphenols on growth of human gut bacteria and their adhesion to enterocytes was investigated. the influence on the growth of a probiotic (lactobacillus rhamnosus), a commensal (escherichia coli) and two pathogenic bacteria (staphylococcus aureus, salmonella typhimurium) was determined, together with effects on adhesion of pathogenic and probiotic bacteria to cultured caco-2 cells. all polyphenols, except rutin, were found to affect the viability of representative g ... | 2008 | 18456359 |
| effect of glucose in removal of microcystin-lr by viable commercial probiotic strains and strains isolated from dadih fermented milk. | the removal of the cyanobacterial peptide toxin microcystin-lr at 4 and 37 degrees c by six commercial probiotic strains and lactobacillus plantarum strains is-10506 and is-20506 isolated from dadih, indonesian traditional fermented milk, was assessed in this study. the aim was to evaluate the main factors influencing the viability and metabolic activity of the probiotic strains, as well as their capacity to remove microcystin-lr. both l. plantarum strains isolated from dadih, as well as bifidob ... | 2008 | 18459790 |
| probiotics, prebiotics, and synbiotics. | according to the german definition, probiotics are defined viable microorganisms, sufficient amounts of which reach the intestine in an active state and thus exert positive health effects. numerous probiotic microorganisms (e.g. lactobacillus rhamnosus gg, l. reuteri, bifidobacteria and certain strains of l. casei or the l. acidophilus-group) are used in probiotic food, particularly fermented milk products, or have been investigated--as well as escherichia coli strain nissle 1917, certain entero ... | 2008 | 18461293 |
| assessment of the safety, tolerance, and protective effect against diarrhea of infant formulas containing mixtures of probiotics or probiotics and prebiotics in a randomized controlled trial. | probiotics and prebiotics are considered to be beneficial to the gastrointestinal health of infants. | 2008 | 18469260 |
| oral probiotic and prevention of pseudomonas aeruginosa infections: a randomized, double-blind, placebo-controlled pilot study in intensive care unit patients. | preventing carriage of potentially pathogenic micro-organisms from the aerodigestive tract is an infection control strategy used to reduce the occurrence of ventilator-associated pneumonia in intensive care units. however, antibiotic use in selective decontamination protocols is controversial. the purpose of this study was to investigate the effect of oral administration of a probiotic, namely lactobacillus, on gastric and respiratory tract colonization/infection with pseudomonas aeruginosa stra ... | 2008 | 18489775 |
| effect of probiotic lactobacillus rhamnosus gg intervention on global serum lipidomic profiles in healthy adults. | to investigate the effect of three weeks' intervention with a probiotic lactobacillus rhamnosus gg (lgg) bacteria on global serum lipidomic profiles and evaluate whether the changes in inflammatory variables (crp, tnf-alpha and il-6) are reflected in the global lipidomic profiles of healthy adults. | 2008 | 18506924 |
| impact of luxs and suppressor mutations on the gastrointestinal transit of lactobacillus rhamnosus gg. | it is generally believed that probiotic bacteria need to survive gastrointestinal transit to exert a health-promoting effect. in this study, a genuine luxs mutant and a luxs mutant containing unknown suppressor mutations of the probiotic strain lactobacillus rhamnosus gg were compared to the wild type for survival and persistence in the murine gastrointestinal tract. the luxs enzyme, catalyzing the production of the autoinducer-2 signaling molecule, also forms an integral part of the activated m ... | 2008 | 18539797 |
| genome sequence and characteristics of lrm1, a prophage from industrial lactobacillus rhamnosus strain m1. | prophage lrm1 was induced with mitomycin c from an industrial lactobacillus rhamnosus starter culture, m1. electron microscopy of the lysate revealed relatively few intact bacteriophage particles among empty heads and disassociated tails. the defective siphoviridae phage had an isometric head of approximately 55 nm and noncontractile tail of about 275 nm with a small baseplate. in repeated attempts, the prophage could not be cured from l. rhamnosus m1, nor could a sensitive host be identified. s ... | 2008 | 18539811 |
| the probiotic lactobacillus gg may augment intestinal host defense by regulating apoptosis and promoting cytoprotective responses in the developing murine gut. | necrotizing enterocolitis (nec) remains a leading cause of morbidity and mortality in preterm infants. although its pathogenesis is poorly understood, inappropriate apoptosis of the mucosal epithelia has been implicated. recent clinical trials have shown that probiotics may reduce the incidence of nec, and probiotics have been shown to suppress intestinal epithelial apoptosis in cultured cells. however, little is known about their mechanism of action in the developing intestine in vivo. here, we ... | 2008 | 18552706 |
| the probiotic lactobacillus acidophilus stimulates chloride/hydroxyl exchange activity in human intestinal epithelial cells. | probiotics are viable nonpathogenic microorganisms that are considered to confer health benefits to the host. recent studies indicated that some lactobacillus species function as probiotics and have been used as alternative treatments for diarrhea, which occurs due to increased secretion, decreased absorption, or both. however, the direct effects of probiotics on intestinal electrolyte absorption are not known. therefore, we examined the effects of lactobacillus on luminal chloride/hydroxyl (cl( ... | 2008 | 18567760 |
| viability of lactobacillus rhamnosus r0011 in an apple-based fruit juice under simulated storage conditions at the consumer level. | lactobacillus rhamnosus r0011 was inoculated in an apple-pear-raspberry juice blend at 4.5 x 10(9) cfu/250 ml portion, and viability was followed during storage in conditions simulating consumer use. study parameters were age of the juice (2 or 4 wk), sampling size (one to three 250-ml portions), incubation temperature (between 2 and 7 degrees c), and atmosphere (aerobic compared with anaerobic). dependent variables were ph, dissolved oxygen (do) level, and viable count. the do level in the unop ... | 2008 | 18577004 |
| effects of multispecies probiotic combination on helicobacter pylori infection in vitro. | probiotic bacteria alleviate many gastrointestinal symptoms, but the current trend of combining bacteria for additional benefit may make their effects more complex. we characterize four probiotics and their combination in terms of pathogen adhesion, barrier function, cell death, and inflammatory response in helicobacter pylori-infected epithelial cells. h. pylori-infected caco-2 cells were pretreated with lactobacillus rhamnosus gg, lactobacillus rhamnosus lc705, propionibacterium freudenreichii ... | 2008 | 18579692 |
| single bioreactor gastrointestinal tract simulator for study of survival of probiotic bacteria. | the aim of the present study was to design an in vitro model system to evaluate the probiotic potential of food. a single bioreactor system-gastrointestinal tract simulator (gits) was chosen for process simulation on account of its considerable simplicity compared to multi-vessel systems used in previous studies. the bioreactor was evaluated by studying the viability of four known probiotic bacteria (lactobacillus acidophilus la-5, lactobacillus johnsonii ncc 533, lactobacillus casei strain shir ... | 2008 | 18581109 |
| probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro. | the use of probiotic bacteria is increasing worldwide and at least some of them can transiently colonize the oral cavity. several studies have shown that probiotic bacteria, which are often thought of in relation only to intestinal health, can also affect the oral ecology, but the mechanisms for this are largely unknown. the aim of this study was to investigate in vitro if the probiotic bacteria used in commercial products affect the protein composition of the salivary pellicle and the adherence ... | 2008 | 18582334 |
| [safety assessment of starters culture bacteria in fermented milk in china]. | in this study, we analysised the safety of 137 strains of starters culture bacteria in fermented milk between 2000 and 2006 in china. the study was designed to evaluate the acute oral toxicity of 137 strains to mice, and 31 of 137 strains for minimum inhibitory concentrations (mic) of 20 kinds of antibiotics by broth micro-dilution method. the result indicated that except a strain of lactobacillus rhamnosus, other probiotics were non-pathogenic and safe for human consumption. 31 strains of probi ... | 2008 | 18589606 |
| [analysis of different lactobacillus rhamnosus by random amplified polymorphic dna]. | we developed a molecular method to mark and differentiate lactobacillus rhamnosus strains and to analyze genetic diversity among isolates from different sources. | 2008 | 18590225 |
| long-term safety and impact on infection rates of postnatal probiotic and prebiotic (synbiotic) treatment: randomized, double-blind, placebo-controlled trial. | live probiotic bacteria and dietary prebiotic oligosaccharides (together termed synbiotics) increasingly are being used in infancy, but evidence of long-term safety is lacking. in a randomized, placebo-controlled, double-blind trial, we studied the safety and long-term effects of feeding synbiotics to newborn infants. | 2008 | 18595980 |
| carboxymethyl high amylose starch: chitosan self-stabilized matrix for probiotic colon delivery. | a new hydrophilic tablet dosage system based on an ionic self-stabilization of a carboxylated (carboxymethyl high amylose starch, cm-has) and an amino (chitosan) excipient was proposed for probiotic colon delivery. cm-has (protonated and compacted in acidic medium) ensures gastro-protection and chitosan (low soluble in intestinal media) prevents early release of lactobacillus rhamnosus bacteria. thus, in cm-has:chitosan monolithic tablets, increasing percentage and molecular weight (mw) of chito ... | 2008 | 18602991 |
| antibacterial action of selenium-enriched probiotics against pathogenic escherichia coli. | the purpose of this study was to evaluate the inhibitory activity of selenium-enriched probiotics against pathogenic escherichia coli (e. coli) in vitro and in vivo. escherichia coli was co-cultured in vitro with each probiotic strain individually, and a mixture of the four strains and its population was counted at various time points. we also collected a cell-free culture supernatant (cfcs) of each probiotic strain and the four-strain mix to examine their antibacterial activity, using the cylin ... | 2009 | 18612820 |
| live lactobacillus rhamnosus and streptococcus pyogenes differentially regulate toll-like receptor (tlr) gene expression in human primary macrophages. | macrophages are phagocytes that recognize bacteria and subsequently activate appropriate innate and adaptive immune responses. tlrs are essential in identifying conserved bacterial structures and in initiating and mediating innate immune responses. in this work, we have characterized tlr gene expression in human monocyte-derived macrophages in response to stimulation with two live gram-positive bacteria, a human commensal and probiotic lactobacillus rhamnosus gg (lgg), and an important human pat ... | 2008 | 18625909 |
| effect of specific colostral antibodies and selected lactobacilli on the adhesion of helicobacter pylori on ags cells and the helicobacter-induced il-8 production. | helicobacter pylori infection is the most common cause of gastritis, gastric ulcer and adenocarcinoma. it has proven difficult to cure because of its capability to develop strains resistant to antibiotics. the effect of three strains of lactic acid bacteria (lab) and bovine colostral preparations on the adhesion of h. pylori nctc 11637 on gastric adenocarcinoma (ags) cells and on the interleukin (il)-8 production was studied. before infection, h. pylori were pretreated with lactobacillus plantar ... | 2008 | 18627549 |
| supplementation with lactobacillus rhamnosus or bifidobacterium lactis probiotics in pregnancy increases cord blood interferon-gamma and breast milk transforming growth factor-beta and immunoglobin a detection. | this study explored the effects of maternal probiotic supplementation on immune markers in cord blood (cb) and breast milk. | 2008 | 18631345 |
| probiotic properties of lactic acid bacteria isolated from stool samples of longevous people in regions of hotan, xinjiang and bama, guangxi, china. | a total of 567 lactic acid bacteria (lab) strains were isolated from the stool samples of longevous people in regions of hotan, xinjiang and bama, guangxi, china. in order to reduce the number of strains for further examinations, 36 isolates were screened out for further examination whilst the other strains, which had lower probiotic properties, were not suitable for yogurt production due to the absence of growth in ph 3.5 mrs medium and no curding during fermentation, and so were excluded. the ... | 2008 | 18634896 |
| lactic acid fermentation in a recycle batch reactor using immobilized lactobacillus casei. | lactic acid production by recycle batch fermentation using immobilized cells of lactobacillus casei subsp. rhamnosus was studied. the culture medium was composed of whey treated with an endoprotease, and supplemented with 2.5 g/l of yeast extract and 0.18 mm mn(2+) ions. the fermentation set-up comprised of a column packed with polyethyleneimine-coated foam glass particles, pora-bact a, and connected with recirculation to a stirred tank reactor vessel for ph control. the immobilization of l. cas ... | 1997 | 18636595 |
| specific strains of probiotic bacteria are efficient in removal of several different cyanobacterial toxins from solution. | the ability of specific strains of probiotic bacteria to remove the pure cyanobacterial peptide toxins microcystin-lr, -rr, -lf, and a combination of microcystins from the cyanobacterial extracts microcystis pcc 7820 and nies 107, as well as the cyanobacterial cytotoxin cylindrospermopsin, from aqueous solutions was assessed. the probiotic bacterial strains studied were lactobacillus rhamnosus strains gg and lc-705, bifidobacterium lactis strains 420 and bb12 and bifidobacterium longum 46, all p ... | 2008 | 18639912 |
| microbiological, chemical, and sensory characteristics of swiss cheese manufactured with adjunct lactobacillus strains using a low cooking temperature. | the effect of nonstarter lactobacillus adjunct cultures on the microbial, chemical, and sensory characteristics of swiss cheese manufactured using the "kosher make procedure" was investigated. the kosher make procedure, which uses a lower cooking temperature than traditional swiss cheese making, is used by many american cheese manufacturers to allow for kosher-certified whey. cheeses were manufactured using a commercial starter culture combination and 1 of 3 non-starter lactobacillus strains pre ... | 2008 | 18650271 |
| the role of luminal factors in the recovery of gastric function and behavioral changes after chronic helicobacter pylori infection. | the role of chronic infections, such as helicobacter pylori (hp), to produce sustained changes in host physiology remains controversial. in this study, we investigate whether the antigenic or bacterial content of the gut, after hp eradication, influences the changes in gut function induced by chronic hp infection. mice were infected with hp for 4 mo and then treated with antibiotics or placebo for 2 wk. gastric emptying was measured using videofluoroscopy, feeding behavior using a 24-h feeding s ... | 2008 | 18653723 |
| lactobacillus rhamnosus l60, a potential probiotic isolated from the human vagina. | the vagina has been increasingly viewed as an "ecosystem" whose normal microflora help protect it from invading pathogens, including those that cause urinary tract infections and sexually transmitted diseases. we tested new strains of lactobacilli as potential probiotics for maintenance of urogenital tract health, as well as prevention and therapy of urogenital infections. a strain of lactobacilli isolated from the vagina of nonpregnant, healthy, premenopausal women was identified as lactobacill ... | 2008 | 18654035 |
| protective effects of lactobacillus reuteri and bifidobacterium infantis in murine models for colitis do not involve the vagus nerve. | the vagus nerve is an important pathway signaling immune activation of the gastrointestinal tract to the brain. probiotics are live organisms that may engage signaling pathways of the brain-gut axis to modulate inflammation. the protective effects of lactobacillus reuteri (lr) and bifidobacterium infantis (bi) during intestinal inflammation were studied after subdiaphragmatic vagotomy in acute dextran sulfate sodium (dss) colitis in balb/c mice and chronic colitis induced by transfer of cd4(+) c ... | 2008 | 18667710 |
| probiotic lactobacilli for urogenital health in women. | the microbiota of the vagina form mostly from ascension of microbes from the rectal area. the numbers and types of microbes fluctuates with hormone levels, sexual contact, douching, and diet, yet the basic composition is relatively simple, with lactobacilli dominant in healthy females. the depletion of these organisms in women susceptible to urinary and vaginal infections, raised the question of whether artificial supplementation of lactobacilli could lower infection rates. to date, a 2 strain c ... | 2008 | 18685506 |
| effect of acute mucosal exposure to lactobacillus rhamnosus gg on human colonic smooth muscle cells. | to define whether human colonic mucosa exposure to lactobacillus rhamnosus gg (lgg), american type culture collection (atcc) 53103, may influence intestinal muscle cell contractility. | 2008 | 18685510 |
| a new chance of preventing winter diseases by the administration of synbiotic formulations. | the efficacy of probiotics is currently well documented with regard to the improvement of gastrointestinal functions, whereas their potential role in the prevention of infectious respiratory diseases has not been sufficiently analyzed. purpose of the study and methods: a 3-stage prospective, randomized, double blind, placebo-controlled study was carried out with several synbiotic preparations containing 3 to 5 strains of lactobacillus plantarum, lactobacillus rhamnosus, and bifidobacterium lacti ... | 2008 | 18685511 |
| microbial content and antibiotic susceptibility of bacterial isolates from yoghurts. | a total of 83 dairy products form the greek market were examined for their bacterial populations and results were compared with the label information. the antibiotic susceptibility of the bacterial isolates was also examined. the status labeling of the yoghurts and the dairy desserts was found insufficient. the 89.8% of the cocci was found susceptible against all the 12 antibiotics examined. lactobacillus spp. strains exhibited some kind of resistance to one or more antibiotics. the isolates of ... | 2008 | 18686111 |
| randomized, double-blind, placebo-controlled study of oral lactobacilli to improve the vaginal flora of postmenopausal women. | the purpose of this randomized, double-blind, placebo-controlled study was to evaluate the influence of the orally administered probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 on the quality of the vaginal flora in postmenopausal women. | 2008 | 18701205 |
| bifidobacterium longum pl03, lactobacillus rhamnosus kl53a, and lactobacillus plantarum pl02 in the prevention of antibiotic-associated diarrhea in children: a randomized controlled pilot trial. | to determine the efficacy of a combination of bifidobacterium longum pl03, lactobacillus rhamnosus kl53a and lactobacillus plantarum pl02 for the prevention of antibiotic-associated diarrhea in children. | 2008 | 18701826 |
| spray bacteriotherapy decreases middle ear fluid in children with secretory otitis media. | secretory otitis media (som) is characterised by persistent fluid in the middle ear cavity, but the cause is unknown. we investigated the clinical, bacteriological and immunological effects of treatment with probiotic bacteria on som. | 2009 | 18713796 |
| lactobacillus rhamnosus gg and lactobacillus casei suppress escherichia coli-induced chemokine expression in intestinal epithelial cells. | recently, some strains of lactic acid bacteria (lab) have been reported to prevent the development of atopic dermatitis and to improve allergic symptoms, especially in young children. however, the mechanisms involved in these effects are not fully understood. intestinal microbiota play critical roles in the development of host immune development and are recognized and regulated by the host through intestinal epithelial cells (iecs). we thus hypothesized that lab influence the host immune system ... | 2009 | 18716403 |
| diversity of lactic acid bacteria in two flemish artisan raw milk gouda-type cheeses. | pcr-denaturing gradient gel electrophoresis (pcr-dgge) was used to study the diversity of lactic acid bacteria (lab) in two flemish artisan raw milk gouda-type cheeses. in parallel, conventional culturing was performed. isolates were identified using (gtg)(5)-pcr and sequence analysis of 16s rrna and phes genes. discriminant analysis revealed some differences in overall lab diversity between the two batches and between the two cheeses. within each batch, the diversity of 8- and 12-week-old chees ... | 2008 | 18721684 |
| adherence of clinically isolated lactobacilli to human cervical cells in competition with neisseria gonorrhoeae. | lactobacilli are normal inhabitants of our microbiota and are known to protect against pathogens. neisseria gonorrhoeae is a human specific pathogenic bacterium that colonises the urogenital tract where it causes gonorrhoea. in this study we analysed early interactions between lactobacilli and gonococci and investigated how they compete for adherence to human epithelial cervical cells. we show that lactobacilli adhere at various levels and that the number of adherent bacteria does not correlate ... | 2008 | 18761100 |
| a differential effect of 2 probiotics in the prevention of eczema and atopy: a double-blind, randomized, placebo-controlled trial. | the role of probiotics in prevention of allergic disease is still not clearly established, although early reports suggested lactobacillus gg halved the risk of eczema at 2 years. | 2008 | 18762327 |
| gateways to clinical trials. | 11d10, 9vpnc-mncc; adalimumab, adefovir dipivoxil, alefacept, aln-rsv01, ame-133, amg-317, aminolevulinic acid methyl ester, amlodipine besylate/atorvastatin calcium, anisodamine, anti-il-5 receptor antibody, apremilast, aripiprazole, atacicept, atazanavir sulfate, atrasentan; banoxantrone, bevacizumab, bibw-2992, binodenoson, bms-387032; cac10, caldaret hydrate, cd-np, ceftobiprole medocaril, celivarone fumarate, certolizumab pegol, cholesteryl hydrophobized polysaccharide-her2 protein complex, ... | 2008 | 18773127 |
| manipulation of intestinal microbial flora for therapeutic benefit in inflammatory bowel diseases: review of clinical trials of probiotics, pre-biotics and synbiotics. | pathogenesis of inflammatory bowel diseases(ulcerative colitis, crohn's disease and pouchitis) includes an abnormal immunological response to disturbed intestinal microflora. therapeutic strategies are designed to intervene in these abnormal host microbial communications. a novel approach in the last decade has been to use other bacteria or selective foods to induce beneficial bacteria to normalize inflammation. in this review we discuss rationale for such use and describe 46 clinical trials gle ... | 2008 | 18782075 |
| the growth and recovery of an exopolysaccharide-producing lactobacillus rhamnosus culture on growth media containing apple juice or molasses. | | 2008 | 18802323 |
| probiotics for the treatment or prevention of eczema. | | 2009 | 18804270 |
| efficacy of high-dose lactobacillus rhamnosus gg in controlling acute watery diarrhea in indian children: a randomized controlled trial. | to evaluate the effective dose of lactobacillus rhamnosus gg (lgg) as probiotic in acute watery diarrhea (awd) in indian children. | 2009 | 18813028 |
| effects of lactobacillus gg treatment during pregnancy on the development of fetal antigen-specific immune responses. | several clinical trials suggest that probiotics may have a role in the prevention of eczema. the optimal timing and mechanisms underlying this intervention are not clear. in particular it is not known whether such treatment works during pregnancy or whether postnatal exposure is important. | 2008 | 18823310 |
| the role of lactobacillus casei rhamnosus lcr35 in restoring the normal vaginal flora after antibiotic treatment of bacterial vaginosis. | to evaluate the efficacy of additional topical lactobacillus casei rhamnosus (lcr35) subsequent to antibiotic treatment of bacterial vaginosis (bv) to restore the normal vaginal flora. | 2008 | 18823487 |
| effects of copper supplement on growth and viability of strains used as starters and adjunct cultures for emmental cheese manufacture. | to determine the effects of supplemented copper (cu2+) on growth and viability of strains used as starters and adjunct cultures for emmental cheese manufacture. | 2008 | 18843790 |
| effectiveness of vaginal administration of lactobacillus rhamnosus following conventional metronidazole therapy: how to lower the rate of bacterial vaginosis recurrences. | eighty-four patients with bacterial vaginosis diagnosed according to amsel's criteria were randomized to receive either oral metronidazole 500 mg twice a day for seven days, or one vaginal tablet containing freeze-dried lactobacillus rhamnosus once a week at bedtime for two months starting one week after the last antibiotic administration. followup was performed at days 30, 90 and 180. chi-squared analysis showed a significant difference between the two treatment groups at day 90 (p = 0.05). saf ... | 2008 | 18843900 |
| [efficacy of lactobacillus rhamnosus gr-1 and of lactobacillus reuteri rc-14 in the treatment and prevention of vaginoses and bacterial vaginitis relapses]. | the aim of this study was to investigate the efficacy of the use of lactobacillus rhamnosus gr-1 and of lactobacillus reuteri rc-14 administrated orally in the treatment and prevention of vaginoses and bacterial vaginitis relapses. | 2008 | 18854803 |
| live and ultraviolet-inactivated lactobacillus rhamnosus gg decrease flagellin-induced interleukin-8 production in caco-2 cells. | probiotics are widely used in the treatment and prevention of gastrointestinal problems. however, in some immune-compromised populations, the administration of live microorganisms may not be appropriate. a potential alternative to live microorganisms is to inactivate them as long as the beneficial function is retained. we hypothesized that uv-inactivated lactobacillus rhamnosus gg (lgg) could downregulate interleukin-8 (il-8) production in intestinal epithelial cells stimulated by the pathogenic ... | 2008 | 18936229 |
| the influence of lactobacillus rhamnosus lc705 together with propionibacterium freudenreichii ssp. shermanii js on potentially carcinogenic bacterial activity in human colon. | the bacterial enzymes beta-glucosidase, beta-glucuronidase, and urease may contribute to the development of colon cancer by generating carcinogens. a reduction in the activity of these enzymes by certain lactic acid bacteria is considered to be beneficial. this study examined fecal beta-glucosidase, beta-glucuronidase, and urease activities during administration of lactobacillus rhamnosus lc705 (lc705) together with propionibacterium freudenreichii ssp shermanii js (pjs). thirty-eight healthy me ... | 2008 | 18945506 |
| effects of probiotic bacteria and their genomic dna on th1/th2-cytokine production by peripheral blood mononuclear cells (pbmcs) of healthy and allergic subjects. | among the factors potentially involved in the increased prevalence of allergic diseases, modification of the intestinal flora or lack of microbial exposure during childhood has been proposed. t(h)2-cytokines increase the production of ige and stimulate mast cells and eosinophils, whereas t(h)1-cytokines, such as ifn-gamma, may suppress ige synthesis and stimulate the expression of the secretory piece of iga. thus, a dysregulation in the expression of t(h)1- and t(h)2-cytokines may contribute to ... | 2008 | 18950596 |
| anticaries effect of compounds extracted from galla chinensis in a multispecies biofilm model. | galla chinensis is a leaf gall known to have some antibacterial effects. using an in vitro biofilm model of dental plaque, the present study aimed to evaluate the anticaries effects of galla chinensis and its chemical fractions. | 2008 | 18954351 |
| sugar fermentation in probiotic bacteria--an in vitro study. | food supplemented with probiotic bacteria is a rapidly growing sector of the market. the aim of the present study was to evaluate and compare the acid production of selected probiotic strains available in commercial products. | 2008 | 18954354 |
| lactobacillus rhamnosus lc705 together with propionibacterium freudenreichii ssp shermanii js administered in capsules is ineffective in lowering serum lipids. | the aim of this study was to examine the effect of the administration of lactobacillus rhamnosus strain lc705 and propionibacterium freudenreichii ssp shermanii strain js in capsules on serum cholesterol and triglyceride levels in mildly or moderately hypercholesterolemic men. | 2008 | 18978162 |
| lactic acid bacteria as novel adjuvant systems for prevention and treatment of atopic diseases. | allergic diseases have become the new epidemic in westernized countries. several factors including reduction of microbial contact early in life may have contributed to this dramatic development. as conventional immunotherapy has obvious limitations, there is a need for the development of new prophylactic and therapeutic treatment approaches. in this respect, lactic acid bacteria have received increasing attention as potential treatment tools against allergy. in this review, different treatment s ... | 2008 | 18978472 |
| specific probiotics in reducing the risk of acute infections in infancy--a randomised, double-blind, placebo-controlled study. | a randomised, double-blind, placebo-controlled study was conducted to determine whether probiotics might be effective in reducing the risk of infections in infancy. infants requiring formula before the age of 2 months were recruited from community well-baby clinics. infant formula supplemented with the probiotics lactobacillus rhamnosus gg and bifidobacterium lactis bb-12 or placebo was administered daily until the age of 12 months. incidence of early infections (before the age of 7 months) and ... | 2009 | 18986600 |
| effect of different prebiotics on the fermentation kinetics, probiotic survival and fatty acids profiles in nonfat symbiotic fermented milk. | the simultaneous effects of different binary co-cultures of lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus rhamnosus and bifidobacterium lactis with streptococcus thermophilus and of different prebiotics on the production of fermented milk were investigated in this paper. in particular, we determined and compared the kinetics of acidification of milk either as such or supplemented with 4% (w/w) maltodextrin, oligofructose and polydextrose, as well as the probiotic survival, c ... | 2009 | 19000641 |
| probiotics and dietary counselling contribute to glucose regulation during and after pregnancy: a randomised controlled trial. | balanced glucose metabolism ensures optimal fetal growth with long-term health implications conferred on both mother and child. we examined whether supplementation of probiotics with dietary counselling affects glucose metabolism in normoglycaemic pregnant women. at the first trimester of pregnancy 256 women were randomised to receive nutrition counselling to modify dietary intake according to current recommendations or as controls; the dietary intervention group was further randomised to receiv ... | 2009 | 19017418 |
| intra-individual diversity and similarity of salivary and faecal microbiota. | in the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (dgge)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individually in two different niches - the oral cavity and the colon - from ten adult volunteers consuming probiotics. the predominant bacterial microbiota, clostridium coccoides-eubacteri ... | 2008 | 19018030 |