| functional analysis of luxs in the probiotic strain lactobacillus rhamnosus gg reveals a central metabolic role important for growth and biofilm formation. | quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. ai-2 is suggested to be a universal bacterial signaling molecule synthesized by the luxs enzyme, which forms an integral part of the activated methyl cycle. we have previously reported that the well-document ... | 2007 | 17098890 |
| the results of a randomized controlled trial to evaluate the effect of lactobacillus rhamnosus. | | 2006 | 17105543 |
| prospective, randomized controlled trial on lactobacillus rhamnosus in infants with moderate to severe atopic dermatitis. | a reduction of symptoms of atopic dermatitis (ad) in small infants by the administration of lactobacillus rhamnosus has been reported in a few studies. one study with older children and adolescents failed to show any effect. | 2006 | 17107398 |
| probiotic bacteria as potential detoxification tools: assessing their heavy metal binding isotherms. | dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. specific probiotic bacteria may have properties that enable them to bind toxins from food and water. we assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial screening step to identify strains for heavy metal decontamination in food and intestinal models. binding isotherms for cadmium and lead were characterized for lactobacillus rhamnosus lc ... | 2006 | 17110980 |
| effects of anti-helicobacter pylori treatment and probiotic supplementation on intestinal microbiota. | the aims of this study were (i) to evaluate the effect of recommended antimicrobial treatment of helicobacter pylori infection, consisting of clarithromycin, amoxicillin and lansoprazole, on intestinal microbiota and (ii) to determine the ability of a probiotic combination containing lactobacillus rhamnosus gg, l. rhamnosus lc705, propionibacterium freudenreichii ssp. shermanii js and bifidobacterium breve bb99 to prevent treatment-induced alterations in the intestinal microbiota. faecal samples ... | 2007 | 17141481 |
| effect of germination and thermal treatments on folates in rye. | effects of germination conditions and thermal processes on folate contents of rye were investigated. total folate contents were determined microbiologically with lactobacillus rhamnosus (atcc 7469) as the growth indicator organism, and individual folates were determined by high-performance liquid chromatography after affinity chromatographic purification. germination increased the folate content by 1.7-3.8-fold, depending on germination temperature, with a maximum content of 250 micro g/100 g dr ... | 2006 | 17147441 |
| lactobacillus fermentum br11, a potential new probiotic, alleviates symptoms of colitis induced by dextran sulfate sodium (dss) in rats. | current treatments for inflammatory bowel disease (ibd) are relatively ineffective. recently, probiotics have emerged as a potential treatment modality for numerous gastrointestinal disorders, including ibd. few probiotics, however, have undergone appropriate preclinical screening in vivo. the current study compared the effects of four candidate probiotics on development of dextran sulfate sodium (dss)-induced colitis in rats. sprague dawley rats were gavaged 1 ml of the potential probiotic (1 x ... | 2007 | 17150273 |
| [lactofem--using of vital lactic acid bacteria in the cases of vaginal microflora imbalance]. | the authors describe the microbiological content of the vaginal ecosystem in healthy, reproductive aged women, as well as women with different types of vaginitis, and the recovering possibilities that lactofem offers. it contains vital h2o2 - producing lactobacillus acidophilus and lactobacillus rhamnosus. | 2006 | 17168486 |
| growth of probiotic lactobacilli in the presence of oleic acid enhances subsequent survival in gastric juice. | the effect of inclusion of various c18 fatty acids with 0-2 double bonds in either cis or trans configuration on lactobacillus rhamnosus gg survival was analysed in simulated gastric juice at ph 2.5. the incorporation of tween 80 (1 g l-1) in the growth media enhanced subsequent survival of stationary-phase cultures up to 1000-fold following 90 min acid exposure compared with controls grown without tween 80. there was a significant (p<0.05) increase in bacterial content of oleic acid [c18:1 (9c) ... | 2007 | 17185558 |
| allergy in marathon runners and effect of lactobacillus gg supplementation on allergic inflammatory markers. | we studied the prevalence of asthma and allergy in non-elite marathon runners and investigated the effects of probiotic supplementation on allergic inflammatory markers. | 2007 | 17196811 |
| probiotics and prebiotic galacto-oligosaccharides in the prevention of allergic diseases: a randomized, double-blind, placebo-controlled trial. | the increase in allergic diseases is attributed to a relative lack of microbial stimulation of the infantile gut immune system. probiotics, live health-promoting microbes, might offer such stimulation. | 2007 | 17208601 |
| synergistic effects of lactobacillus rhamnosus zdy114 and bovine colostrums on the immunological function of mouse in vivo and in vitro. | the synergistic effects of lactobacillus rhamnosus and bovine colostrums on the immunity of mice in vivo and in vitro were investigated. eight- to ten-week-old mice were used for two series experiments; one part of mice were immunocompromised by intraperitoneal injections of cyclophosphamide. in series i, immunocompromised mice were continuously fed with diet a (l. rhamnosus zdy114 5 x 10(7) cfu/kg), b (bovine colostrums 0.5 g/kg), c (combination of diet a and b), and d (sterile saline) for 4 we ... | 2007 | 17225092 |
| a randomized double-blind placebo-controlled trial of lactobacillus gg for abdominal pain disorders in children. | functional abdominal pain disorders (fapd) are common in school-aged children; however, there is no reliable treatment. | 2007 | 17229242 |
| effect of lactobacillus challenge on gardnerella vaginalis biofilms. | bacterial vaginosis (bv) is the most common infectious condition in women. it is caused primarily by anaerobic bacteria which rapidly form biofilms recalcitrant to antibiotic treatment, elevate vaginal ph, induce inflammatory processes and displace indigenous lactobacilli from the vault. gardnerella vaginalis is commonly associated with these infections. microscopy analysis showed that within 72 h, viable g. vaginalis covered a surface area of 567 microm(2), reached a depth of 16 microm and a de ... | 2007 | 17234391 |
| probiotic effects on 5-fluorouracil-induced mucositis assessed by the sucrose breath test in rats. | the sucrose breath test (sbt) was employed to noninvasively assess the efficacy of probiotics in 5-fluorouracil (5-fu)-induced intestinal mucositis. dark agouti rats were allocated to 5 groups (n = 10): 5-fu + l. fermentum br 11, 5-fu + l. rhamnosus gg, 5-fu + b. lactis bb 12, 5-fu + skim milk (sm), and saline + sm. probiotics were administered by oral gavage for 10 days. mucositis was induced on day 7 by intraperitoneal injection of 5-fu (150 mg/kg) or vehicle (saline). rats were sacrificed 72 ... | 2007 | 17237997 |
| ingestion of heat-treated lactobacillus rhamnosus gg prevents development of atopic dermatitis in nc/nga mice. | continuous oral administration of live lactobacillus rhamnosus gg (l. gg) to pregnant subjects with atopic dermatitis and their children, suppressed the frequency of atopic dermatitis. the details of mechanisms and immune systems involved in this suppressive effect, however, remain speculative. | 2007 | 17250703 |
| soluble proteins produced by probiotic bacteria regulate intestinal epithelial cell survival and growth. | increased inflammatory cytokine levels and intestinal epithelial cell apoptosis leading to disruption of epithelial integrity are major pathologic factors in inflammatory bowel diseases. the probiotic bacterium lactobacillus rhamnosus gg (lgg) and factors recovered from lgg broth culture supernatant (lgg-s) prevent cytokine-induced apoptosis in human and mouse intestinal epithelial cells by regulating signaling pathways. here, we purify and characterize 2 secreted lgg proteins that regulate inte ... | 2007 | 17258729 |
| colonisation of the gastrointestinal tract by probiotic l. rhamnosus strains in acute diarrhoea in children. | lactobacillus rhamnosus (573l/1-3) strains are considered effective in the treatment of rotaviral diarrhoea in children. the colonisation of the gastrointestinal (g.i.) tract by the lactobacillus rhamnosus strains and the determining factors are discussed reporting data of a prospective, double-blinded, placebo-controlled randomized study in children between the 2nd month and 6th year of life with acute diarrhoea lasting not longer than 5 days. the examined strains were detected in 37/46 (80.43% ... | 2006 | 17259090 |
| removal of microcystin-lr by strains of metabolically active probiotic bacteria. | the ability of specific strains of probiotic bacteria to remove the cyanobacterial peptide toxin microcystin-lr from aqueous solutions was assessed. lactobacillus rhamnosus strains gg and lc-705, bifidobacterium longum 46, bifidobacterium lactis 420 and bifidobacterium lactis bb12 were shown to be the most effective in toxin removal among 11 tested strains. the highest removal percentage of microcystin-lr was 58.1%, observed with b. lactis bb12 (toxin concentration 100 microg l(-1), 10(10) cfu m ... | 2007 | 17263839 |
| adhesive and chemokine stimulatory properties of potentially probiotic lactobacillus strains. | five lactobacillus plantarum strains and two lactobacillus johnsonii strains, stemming either from african traditionally fermented milk products or children's feces, were investigated for probiotic properties in vitro. the relationship between the hydrophobic-hydrophilic cell surface and adhesion ability to ht29 intestinal epithelial cells was investigated, and results indicated that especially the l. johnsonii strains, which exhibited both hydrophobic and hydrophilic surface characteristics, ad ... | 2007 | 17265871 |
| dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients. | animal studies suggest that prebiotics and probiotics exert protective effects against tumor development in the colon, but human data supporting this suggestion are weak. | 2007 | 17284748 |
| probiotics during the first 7 years of life: a cumulative risk reduction of eczema in a randomized, placebo-controlled trial. | | 2007 | 17289135 |
| influence of macrophage activation on their capacity to bind bacterial antigens studied with atomic force microscopy. | in this work we studied interactions between bacterial antigens and receptors on the surface of macrophages using atomic force microscopy (afm). we used two bacterial cell wall components: lipopolysaccharide (lps) derived from gram-negative escherichia coli and exopolysaccharide (eps) derived from gram-positive lactobacillus rhamnosus. interactions between these bacterial antigens and immune cell receptors were studied in peritoneal macrophages derived from two strains of mice, cba and c3h/j, in ... | 2006 | 17292119 |
| growth of probiotic bacteria and bifidobacteria in a soy yogurt formulation. | soy beverage and cows' milk yogurts were produced with steptococcus thermophilus (atcc 4356) and lactobacillus delbrueckii subsp. bulgaricus (im 025). the drop in ph during fermentation was faster in the soy beverage than in cows' milk, but the final ph values were similar. yogurts were prepared with a yogurt starter in conjunction with either the probiotic bacteria lactobacillus johnsonii ncc533 (la-1), lactobacillus rhamnosus atcc 53103 (gg) or human derived bifidobacteria. the presence of the ... | 2007 | 17292991 |
| antiallergic effects of probiotics. | a considerable part of the western population suffers from some form of allergy, and the incidence is still rising with no sign of an end to this trend. reduced exposure to microbial allergens as a result of our hygienic lifestyle has been suggested as one of the possible causes. it has also been suggested that probiotics may provide safe alternative microbial stimulation needed for the developing immune system in infants. this idea is supported by the fact that allergic infants have been observ ... | 2007 | 17311977 |
| probiotics and prebiotics: effects on diarrhea. | probiotics have preventive as well as curative effects on several types of diarrhea of different etiologies. prevention and therapy (or alleviation) of diarrhea have been successfully investigated for numerous dietary probiotics to establish probiotic properties and to justify health claims (the medicinal use of probiotic food and the therapy of gastrointestinal diseases itself may not be advertised under current food laws). other probiotic microorganisms (e.g., lactobacillus rhamnosus gg, l. re ... | 2007 | 17311979 |
| synergistic antidigestion effect of lactobacillus rhamnosus and bovine colostrums in simulated gastrointestinal tract (in vitro). | probiotics and bovine colostrums had been proven to be beneficial for human health. lactobacillus rhamnosus zdy114 and anti-helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was investigated. either l. rhamnosus or purified igg from immune colostrums was very sensitive in simulated gastric environment and slightly sensitive in simulated intestinal tract. n ... | 2007 | 17318540 |
| genome-shuffling improved acid tolerance and l-lactic acid volumetric productivity in lactobacillus rhamnosus. | genome shuffling is an efficient approach for the rapid improvement of industrially important microbial phenotypes. here we improved the acid tolerance and volumetric productivity of an industrial strain lactobacillus rhamnosus atcc 11443 by genome shuffling. five strains with subtle improvements in ph tolerance and volumetric productivity were obtained from the populations generated by ultraviolet irradiation and nitrosoguanidine mutagenesis, and then they were subjected for recursive protoplas ... | 2007 | 17320995 |
| l-lactic acid production from apple pomace by sequential hydrolysis and fermentation. | the potential of apple pomace (a solid waste from cider and apple juice making factories) as a source of sugars and other compounds for fermentation was evaluated. the effect of the cellulase-to-solid ratio (csr) and the liquor-to-solid ratio (lsr) on the kinetics of glucose and total monosaccharide generation was studied. mathematical models suitable for reproducing and predicting the hydrolyzate composition were developed. when samples of apple pomace were subjected to enzymatic hydrolysis, th ... | 2008 | 17321133 |
| [study of susceptibility of oral bacteria biofilm to traditional chinese drug preventing caries]. | using mbec-assay to assay minimal biofilm eradication concentration (mbec) of galla chinensis and nidus vespae to oral bacterial biofilm. to set up traditional chinese medicine susceptibility pharmacodynamic empirical study methods of oral bacterial biofilm. | 2006 | 17334081 |
| consumption of prebiotic inulin enriched with oligofructose in combination with the probiotics lactobacillus rhamnosus and bifidobacterium lactis has minor effects on selected immune parameters in polypectomised and colon cancer patients. | probiotics (pro) modulate immunity in humans, while the effect of prebiotics (pre) and synbiotics (syn) on the human immune system are not well studied yet. the objective of this study was to investigate whether daily intake of a syn modulates immune functions. in a randomised double-blind, placebo-controlled trial, thirty-four colon cancer patients who had undergone 'curative resection' and forty polypectomised patients participated. subjects of the syn group daily received encapsulated bacteri ... | 2007 | 17349080 |
| treatment of acute otitis media with probiotics in otitis-prone children-a double-blind, placebo-controlled randomised study. | to examine whether probiotics would reduce the occurrence or duration of acute otitis media (aom), or the nasopharyngeal carriage of otitis pathogens in otitis-prone children. | 2007 | 17353072 |
| prevention of antibiotic-associated diarrhoea by a fermented probiotic milk drink. | to study the preventive effect of a milk drink fermented with multistrain probiotics on antibiotic associated diarrhoea (aad). | 2008 | 17356555 |
| lactobacillus rhamnosus gg exacerbates intestinal ulceration in a model of indomethacin-induced enteropathy. | lactobacillus rhamnosus gg (lgg) and bifidobacterium lactis bb12 (bb12) were assessed for their potential to prevent indomethacin-induced ulceration in the small intestine of sprague-dawley rats. rats were gavaged skim milk, lgg, or bb12 twice daily for 14 days. between days 7-14, rats were gavaged indomethacin (indo; 6 mg/kg). at sacrifice, small intestine was scored for ulceration and sampled for histologic, immunohistochemical, and myeloperoxidase (mpo) analyses. indo+lgg-treated rats exhibit ... | 2007 | 17357841 |
| perinatal maternal application of lactobacillus rhamnosus gg suppresses allergic airway inflammation in mouse offspring. | clinical studies indicate that maternal exposure to probiotic bacteria may protect from the development of allergic disease later in life. | 2007 | 17359385 |
| a key role of dendritic cells in probiotic functionality. | disruption of the intestinal homeostasis and tolerance towards the resident microbiota is a major mechanism involved in the development of inflammatory bowel disease. while some bacteria are inducers of disease, others, known as probiotics, are able to reduce inflammation. because dendritic cells (dcs) play a central role in regulating immune responses and in inducing tolerance, we investigated their role in the anti-inflammatory potential of probiotic lactic acid bacteria. | 2007 | 17375199 |
| probiotic lactobacillus rhamnosus gg enhances gastric ulcer healing in rats. | probiotics are widely used as functional foods which have been advocated for the maintenance of gastrointestinal microflora equilibrium and treatment of gastrointestinal disorders. however, studying the role of probiotics in peptic ulcer disease is limited. the aim of the present study is to investigate the effect of a probiotic strain lactobacillus rhamnosus gg on gastric ulcer and to elucidate the mechanisms involved. gastric kissing ulcers were induced in rats by acetic acid (60% v/v). l. rha ... | 2007 | 17395175 |
| determination of folate concentrations in diverse potato germplasm using a trienzyme extraction and a microbiological assay. | folate deficiency is a leading cause of birth defects and is implicated in several other diseases. we are interested in how much folate concentrations vary among potato germplasm. we determined total folate concentrations of potato tubers from 67 cultivars, advanced breeding lines, or wild species. folates were extracted by a tri-enzyme treatment and analyzed by using a lactobacillus rhamnosus microbiological assay. folate concentrations varied from 521 +/- 96 to 1373 +/- 230 ng/g dry weight and ... | 2007 | 17419642 |
| probiotic-induced suppression of allergic sensitization and airway inflammation is associated with an increase of t regulatory-dependent mechanisms in a murine model of asthma. | microbial intestinal colonization in early in life is regarded to play a major role for the maturation of the immune system. application of non-pathogenic probiotic bacteria during early infancy might protect from allergic disorders but underlying mechanisms have not been analysed so far. | 2007 | 17430345 |
| functional analysis of d-alanylation of lipoteichoic acid in the probiotic strain lactobacillus rhamnosus gg. | lipoteichoic acid (lta) is a macroamphiphile molecule which performs several functions in gram-positive bacteria, such as maintenance of cell wall homeostasis. d-alanylation of lta requires the proteins encoded by the dlt operon, and this process is directly related to the charge properties of this polymer strongly contributing to its function. the insertional inactivation of dltd of the probiotic strain lactobacillus rhamnosus gg (atcc 53103) resulted in the complete absence of d-alanyl esters ... | 2007 | 17434999 |
| lactobacillus rhamnosus strain gg reduces aflatoxin b1 transport, metabolism, and toxicity in caco-2 cells. | the probiotic lactobacillus rhamnosus gg is able to bind the potent hepatocarcinogen aflatoxin b1 (afb1) and thus potentially restrict its rapid absorption from the intestine. in this study we investigated the potential of gg to reduce afb1 availability in vitro in caco-2 cells adapted to express cytochrome p-450 (cyp) 3a4, such that both transport and toxicity could be assessed. caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. afb1 level ... | 2007 | 17449679 |
| conjugated linoleic acid conversion by dairy bacteria cultured in mrs broth and buffalo milk. | to evaluate strains of lactobacilli, bifidobacteria and streptococci for their ability to produce conjugated linoleic acid (cla) from free linoleic acid (la). | 2007 | 17451511 |
| protection offered by root-surface restorative materials against biofilm challenge. | the prevalence of root-surface caries is increasing. we hypothesized that some restorative materials are protective against cariogenic challenge on root surfaces. our goal was to study the effects of different restorative materials on root surfaces incubated with an oral biofilm generated in an artificial mouth. a biofilm of streptococcus mutans, streptococcus sobrinus, lactobacillus rhamnosus, and actinomyces naeslundii was co-cultured for 21 days on 24 glass-ionomer cement, resin-modified glas ... | 2007 | 17452563 |
| analysis of the presence of prtr proteinase gene in natural isolates of lactobacillus rhamnosus. | the region of the prtr gene coding for the active site of prtr proteinase was detected in natural isolates of lactobacilli, previously determined as lactobacillus rhamnosus. this region was present in all l. rhamnosus strains with proteolytic activity. the pcr primers used were constructed on the basis of the sequence of the catalytic domain of the prtr proteinase gene. these primers generated in colony-pcr procedure specific 611 1-bp product with dna from natural isolates of l. rhamnosus. no pc ... | 2006 | 17455789 |
| protective role of lactobacilli in shigella dysenteriae 1-induced diarrhea in rats. | studies on lactic acid bacteria exemplify their use against various enteropathogens in vitro. nevertheless, in vivo effects of lactobacillus during shigella infection have not been evaluated. the present study evaluated the effect of lactobacillus rhamnosus and lactobacillus acidophilus on neutrophil infiltration and lipid peroxidation during shigella dysenteriae 1-induced diarrhea in rats. | 2007 | 17483010 |
| probiotic treatment of vancomycin-resistant enterococci: a randomised controlled trial. | to determine whether eating lactobacillus rhamnosus gg (lgg) in the form of commercially available yoghurt improves clearance of vancomycin-resistant enterococci (vre). | 2007 | 17484706 |
| enhancement of gastric mucosal integrity by lactobacillus rhamnosus gg. | the gastric mucosa is frequently exposed to different exogenous and endogenous ulcerative agents. alcoholism is one of the risk factors for the development of mucosal damage in the stomach. this study aimed to assess if a probiotic strain lactobacillus rhamnosus gg (lgg) is capable of protecting the gastric mucosa from acute damage induced by intragastric administration of ethanol. pre-treatment of rats with lgg at 10(9) cfu/ml twice daily for three consecutive days markedly reduced ethanol-indu ... | 2007 | 17499310 |
| development of new probiotics by strain combinations: is it possible to improve the adhesion to intestinal mucus? | we evaluated the ability of commercial probiotic strains (lactobacillus rhamnosus gg, l. rhamnosus lc705, bifidobacterium breve 99, and propionibacterium freudenreichii ssp. shermanii js) to adhere alone or in different combinations to immobilized mucus. probiotic combinations were clearly able to enhance the adhesion of l. rhamnosus gg, l. rhamnosus lc705, and p. freudenreichii ssp. shermanii js. for l. rhamnosus gg and p. freudenreichii js, all the combinations significantly improved adhesion ... | 2007 | 17517710 |
| soluble cd14 and cd83 from human neonatal antigen-presenting cells are inducible by commensal bacteria and suppress allergen-induced human neonatal th2 differentiation. | cd14 is expressed on the cell surface of various antigen-presenting cells, and cd83 is a maturation marker for dendritic cells (dc). cd14 and cd83 are also present as soluble proteins, and both have immunoregulatory functions. we examined whether neonatal cord blood monocytes or dc released soluble cd14 (scd14) or scd83 when exposed to the commensal intestinal bacteria clostridium perfringens, staphylococcus aureus, lactobacillus rhamnosus, escherichia coli, and bacteroides fragilis. we found th ... | 2007 | 17526743 |
| mixed-species biofilm formation by lactic acid bacteria and rice wine yeasts. | we found that species combinations such as lactobacillus casei subsp. rhamnosus ifo3831 and saccharomyces cerevisiae kyokai-10 can form a mixed-species biofilm in coculture. moreover, the kyokai-10 yeast strain can form a biofilm in monoculture in the presence of conditioned medium (cm) from l. casei ifo3831. the active substance(s) in bacterial cm is heat sensitive and has a molecular mass of between 3 and 5 kda. in biofilms from cocultures or cm monocultures, yeast cells had a distinct morphol ... | 2007 | 17526778 |
| media and process parameters affecting the growth, strain ratios and specific acidifying activities of a mixed lactic starter containing aroma-producing and probiotic strains. | the effects of medium-composition and fermentation parameters on the properties of mixed mesophilic starters were studied. the starter was composed of lactococcus lactis ssp. lactis (l. lactis), lactococcus lactis ssp. cremoris (l. cremoris), lactobacillus rhamnosus (lact. rhamnosus) and leuconostoc mesenteroides ssp. cremoris (leuc. cremoris). | 2007 | 17584462 |
| lactobacillus casei rhamnosus lcr35 in children with chronic constipation. | the purpose of the present paper was to evaluate the efficacy of probiotics (lactobacillus casei rhamnosus, lcr35) for treating children with chronic constipation and to compare its effect with magnesium oxide (mgo) and placebo. | 2007 | 17587273 |
| anti-inflammatory effects of probiotic yogurt in inflammatory bowel disease patients. | our aim was to assess anti-inflammatory effects on the peripheral blood of subjects with inflammatory bowel disease (ibd) who consumed probiotic yogurt for 1 month. we studied 20 healthy controls and 20 subjects with ibd, 15 of whom had crohn's disease and five with ulcerative colitis. all the subjects consumed lactobacillus rhamnosus gr-1 and l. reuteri rc-14 supplemented yogurt for 30 days. the presence of putative regulatory t (t(reg)) cells (cd4(+) cd25(high)) and cytokines in t cells, monoc ... | 2007 | 17590176 |
| [effects of the active compounds of m. officinalis on cariogenic bacteria]. | to investigate the effects of the principal biologically active compounds of m. officinalis on the growth and acid generation of the main cariogenic bacteria. | 2007 | 17593830 |
| probiotics for clostridium difficile-associated diarrhea: focus on lactobacillus rhamnosus gg and saccharomyces boulardii. | to review the literature on the use of probiotics to treat or prevent recurrences of clostridium difficile-associated diarrhea (cdad) by replacing normal gastric flora. | 2007 | 17595306 |
| probiotics for the treatment of women with bacterial vaginosis. | this review considers whether probiotics are effective agents for the treatment and/or prevention of bacterial vaginosis (bv). there seems to be an association between the absence of, or low concentrations of, vaginal lactobacilli and the development of bv. many studies have suggested that the presence of h2o2-producing vaginal lactobacilli may protect against bv, although some studies do not support this hypothesis. in-vitro studies have suggested that certain specific strains of lactobacilli a ... | 2007 | 17633390 |
| effects of multispecies probiotic supplementation on intestinal microbiota in irritable bowel syndrome. | a multispecies probiotic has shown beneficial effects in irritable bowel syndrome. in addition, certain other probiotics have demonstrated advantageous effects, but the mechanisms behind this are poorly understood. | 2007 | 17635381 |
| acquired macrolide resistance in the human intestinal strain lactobacillus rhamnosus e41 associated with a transition mutation in 23s rrna genes. | restriction fragment length polymorphism and dna sequencing of polymerase chain reaction (pcr) products showed that a lactobacillus rhamnosus strain of human origin resistant to macrolides, from which no resistance determinants have been detected by specific pcr and microarray screening, contained a heterozygous a-->g transition mutation at position 2058 (escherichia coli numbering) of its 23s rrna genes. | 2007 | 17644348 |
| functional analysis of the fructooligosaccharide utilization operon in lactobacillus paracasei 1195. | the fosabcdxe operon encodes components of a putative fructose/mannose phosphoenolpyruvate-dependent phosphotransferase system and a beta-fructosidase precursor (fose) that are involved in the fructooligosaccharide (fos) utilization pathway of lactobacillus paracasei 1195. the presence of an n-terminal signal peptide sequence and an lpqag cell wall anchor motif in the c-terminal region of the deduced fose precursor amino acid sequence predicted that the enzyme is cell wall associated, indicating ... | 2007 | 17644636 |
| infectivity of lactobacillus rhamnosus and lactobacillus paracasei isolates in a rat model of experimental endocarditis. | the potential pathogenicity of selected (potentially) probiotic and clinical isolates of lactobacillus rhamnosus and lactobacillus paracasei was investigated in a rat model of experimental endocarditis. in addition, adhesion properties of the lactobacilli for fibrinogen, fibronectin, collagen and laminin, as well as the killing activity of the platelet-microbicidal proteins fibrinopeptide a (fp-a) and connective tissue activating peptide 3 (ctap-3), were assessed. the 90 % infective dose (id(90) ... | 2007 | 17644707 |
| preliminary characterization of wild lactic acid bacteria and their abilities to produce flavour compounds in ripened model cheese system. | the aim of this work was to preliminary characterize wild lactic acid bacteria (lab), previously isolated during artisanal pecorino siciliano (ps) cheese-making for technological and flavour formation abilities in a model cheese system. | 2007 | 17650203 |
| probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa. | the aim of this study was to investigate in vitro the protective effect of commercial probiotic strains (bifidobacterium lactis bb12 and lactobacillus rhamnosus lgg) alone and in combination on the adhesion of pathogenic strains as salmonella, clostridium, and escherichia coli to pig intestinal mucus obtained from different intestinal regions. in combination, probiotic strains enhanced each other's adhesion, mainly in large intestinal mucus. treatment of intestinal mucus with bb12 and lgg, alone ... | 2007 | 17657533 |
| lack of effect of lactobacillus gg in breast-fed infants with rectal bleeding: a pilot double-blind randomized controlled trial. | for breast-fed infants with rectal bleeding, maternal restriction of dietary protein such as cow's milk proteins is often recommended; however, poor response to dietary protein restriction is of concern. this pilot study was designed to assess the effectiveness of lactobacillus gg (lgg) in breast-fed infants with rectal bleeding. | 2007 | 17667723 |
| functionality of probiotics - potential for product development. | it is becoming increasingly accepted by consumers that live lactic acid bacteria do exert health benefits when eaten. in addition, it is also becoming recognised that not all probiotic bacteria are equal. it is now no longer just a question of providing sufficient numbers of viable bacteria in a product; industry must also provide proof of efficacy for each strain. in the early 1990s, fonterra embarked on a programme to develop proprietary probiotic strains, and as a result, commercialised two s ... | 2007 | 17684416 |
| elementary mode analysis to study the preculturing effect on the metabolic state of lactobacillus rhamnosus during growth on mixed substrates. | quantification of metabolism through elementary modes offers insights into the working of a metabolic network. we have determined the fluxes of elementary modes through linear optimization using the stoichiometry of the elementary modes as a constraint. we apply this methodology to obtain insights into the effect of preculturing on growth of lactobacillus rhamnosus on medium containing mixed substrates. l. rhamnosus, a microaerophilic organism, produces flavor compounds such as diacetyl and acet ... | 2007 | 17688437 |
| probiotics for treatment of acute diarrhoea in children: randomised clinical trial of five different preparations. | to compare the efficacy of five probiotic preparations recommended to parents in the treatment of acute diarrhoea in children. design randomised controlled clinical trial in collaboration with family paediatricians over 12 months. | 2007 | 17690340 |
| probiotic administration and the incidence of nosocomial infection in pediatric intensive care: a randomized placebo-controlled trial. | to evaluate the efficacy of probiotics in reducing the rates of nosocomial infection in pediatric intensive care. | 2007 | 17693918 |
| lactobacillus-mediated interference of mutans streptococci in caries-free vs. caries-active subjects. | in order to assess whether naturally occurring oral lactobacilli have probiotic properties, lactobacilli were isolated from saliva and plaque from children and adolescents, with or without caries lesions. the interference capacities of these lactobacilli were investigated against a panel of 13 clinical isolates and reference strains of streptococcus mutans and streptococcus sobrinus, as well as against the subject's autologous mutans streptococci, using the agar-overlay technique. lactobacillus- ... | 2007 | 17697171 |
| effect of lactobacillus rhamnosus gg in persistent diarrhea in indian children: a randomized controlled trial. | to evaluate the role of lactobacillus rhamnosus gg (lgg) as probiotic in persistent diarrhea (pd) in children of north bengal, india. | 2007 | 17700424 |
| the effect of early nutritional supplementation with a mixture of probiotic, prebiotic, fiber and micronutrients in infants with acute diarrhea in indonesia. | a randomized double blind clinical trial was conducted to assess the efficacy of a special infant formula containing lactobacillus rhamnosus lmg p-22799 (probiotic: 5 x 10(8) cfu/100ml), inulin (prebiotic: 0.15 g/100ml), dietary fiber (soy polysaccharides: 0.2 g/100ml) and increased amounts of zinc+iron (+0.4 and +0.6 mg/100ml, respectively) as active ingredients for the early dietary management of 58 indonesian well-nourished male infants aged 3-12 months suffering from acute diarrhea with mode ... | 2007 | 17704024 |
| multilocus sequence typing of lactobacillus casei reveals a clonal population structure with low levels of homologous recombination. | robust genotyping methods for lactobacillus casei are needed for strain tracking and collection management, as well as for population biology research. a collection of 52 strains initially labeled l. casei or lactobacillus paracasei was first subjected to rplb gene sequencing together with reference strains of lactobacillus zeae, lactobacillus rhamnosus, and other species. phylogenetic analysis showed that all 52 strains belonged to a single compact l. casei-l. paracasei sequence cluster, togeth ... | 2007 | 17704267 |
| the lactobacillus plantarum strain aca-dc287 isolated from a greek cheese demonstrates antagonistic activity in vitro and in vivo against salmonella enterica serovar typhimurium. | the purpose of this study was to investigate the antibacterial activity of the xynotyri cheese isolate lactobacillus plantarum aca-dc287 using a set of in vitro and in vivo assays. | 2007 | 17714399 |
| are probiotics detectable in human feces after oral uptake by healthy volunteers? | assessment of the presence of probiotic bacteria in feces after oral ingestion. | 2007 | 17721765 |
| enhancement of freezing-resistance of lactobacillus rhamnosus by the application of cell immobilization. | | 2007 | 17726303 |
| efficacy of lactobacillus rhamnosus gg in acute watery diarrhoea of indian children: a randomised controlled trial. | to evaluate the role of lactobacillus rhamnosus gg (lgg) as probiotic in acute watery diarrhoea (awd). | 2007 | 17803667 |
| [evaluation of the effect of lactobacillus rhamnosus probiotic culture added to yogurt over staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes and salmonella enteritidis populations]. | the effect of different types of probiotics present in yogurt over known populations of staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes and salmonella enteritidis was evaluated. the three types of yogurt used were: without added probiotics, with added probiotics (lactobacillus casei crl_431 and l. acidophilus crl_730 chr hansen) and another one with the same probiotics mentioned above and lactobacillus rhamnosus (lr-35) culture. about 10(9) cfu/ ml of each potentially pat ... | 2007 | 17824199 |
| impact of environmental and genetic factors on biofilm formation by the probiotic strain lactobacillus rhamnosus gg. | lactobacillus rhamnosus gg (atcc 53103) is one of the clinically best-studied probiotic organisms. moreover, l. rhamnosus gg displays very good in vitro adherence to epithelial cells and mucus. here, we report that l. rhamnosus gg is able to form biofilms on abiotic surfaces, in contrast to other strains of the lactobacillus casei group tested under the same conditions. microtiter plate biofilm assays indicated that in vitro biofilm formation by l. rhamnosus gg is strongly modulated by culture m ... | 2007 | 17827316 |
| dietary supplementation with lactic acid bacteria attenuates the development of atopic-dermatitis-like skin lesions in nc/nga mice in a strain-dependent manner. | dietary supplementation with lactic acid bacteria (lab) is a potential approach to the prevention and manipulation of allergic diseases such as atopic dermatitis (ad). however, the influence of different bacterial strains and their immunomodulating capacities is still largely unknown. | 2008 | 17848807 |
| il-10-inducing adjuvants enhance sublingual immunotherapy efficacy in a murine asthma model. | il-10-inducing adjuvants could enhance the efficacy of allergy vaccines in establishing allergen-specific tolerance. the aim of this study was to identify such adjuvants using in vitro cultures of human and murine cells and to evaluate them in a therapeutic murine model of sublingual immunotherapy (slit). | 2008 | 17848808 |
| probiotics in the critically ill: handle with care! | | 2007 | 17873787 |
| vaginal lactobacillus microbiota of healthy women in the late first trimester of pregnancy. | this study was undertaken to characterise the dominant species of lactobacillus colonising the vagina of healthy pregnant women, to examine some of their phenotypic and genotypic properties, and to gain a better understanding of the potential role of species, which might be associated with infection-free status. | 2007 | 17877778 |
| nonstarter lactobacillus strains as adjunct cultures for cheese making: in vitro characterization and performance in two model cheeses. | nonstarter lactic acid bacteria are the main uncontrolled factor in today's industrial cheese making and may be the cause of quality inconsistencies and defects in cheeses. in this context, adjunct cultures of selected lactobacilli from nonstarter lactic acid bacteria origin appear as the best alternative to indirectly control cheese biota. the objective of the present work was to study the technological properties of lactobacillus strains isolated from cheese by in vitro and in situ assays. mil ... | 2007 | 17881674 |
| lactobacillus supplementation for diarrhoea related to chemotherapy of colorectal cancer: a randomised study. | 5-fluorouracil (5-fu)-based chemotherapy is frequently associated with diarrhoea. we compared two 5-fu-based regimens and the effect of lactobacillus and fibre supplementation on treatment tolerability. patients diagnosed with colorectal cancer (n=150) were randomly allocated to receive monthly 5-fu and leucovorin bolus injections (the mayo regimen) or a bimonthly 5-fu bolus plus continuous infusion (the simplified de gramont regimen) for 24 weeks as postoperative adjuvant therapy. on the basis ... | 2007 | 17895895 |
| murine immunomodulation of il-10 and il-12 induced by new isolates from avian type 2 lactobacillus acidophilus. | several bacterial and immunogenic factors are involved in the host response to probiotic strains of lactobacillus. here, we report the isolation of new intestinal lactobacilli from chicken, with different immunomodulating properties on lymphoid cells from sjl and c57bl/6 mice. analysis of biochemical markers in the lactobacillus acidophilus cba4p, cba3p, and tpa3p isolates reveal that these bacterial isolates belong to the type 2 prototype, although they differ from each other. the effect of con ... | 2007 | 17898851 |
| effective prophylaxis against rotavirus diarrhea using a combination of lactobacillus rhamnosus gg and antibodies. | rotavirus is a worldwide cause of infectious infantile diarrhea that claims over 600,000 lives annually. recently, two new vaccine candidates have been developed but their efficacy in developing countries, still remains to be proven. oral delivery of specific immunoglobulins provides passive immunity and is a fast acting treatment for rotavirus diarrhea. probiotic bacteria have also gained considerable attention lately as treatment for rotavirus diarrhea. here we report an evaluation of the ther ... | 2007 | 17900343 |
| commensal bacteria modulate cullin-dependent signaling via generation of reactive oxygen species. | the resident prokaryotic microflora of the mammalian intestine influences diverse homeostatic functions of the gut, including regulation of cellular growth and immune responses; however, it is unknown how commensal prokaryotic organisms mechanistically influence eukaryotic signaling networks. we have shown that bacterial coculture with intestinal epithelial cells modulates ubiquitin-mediated degradation of important signaling intermediates, including beta-catenin and the nf-kappab inhibitor ikap ... | 2007 | 17914462 |
| randomized, placebo-controlled trial of lactobacillus rhamnosus gg as treatment of atopic dermatitis in infancy. | some studies have suggested that supplementation of food with lactobacilli may prevent or improve atopic dermatitis in children. this study was designed to investigate the therapeutic effect of lactobacillus rhamnosus gg (lgg) as a food supplement in infants suffering from atopic dermatitis. | 2007 | 17919141 |
| clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota. | irritable bowel syndrome is the most common diagnosis in gastroenterology. trials suggest certain probiotics to be beneficial. | 2008 | 17919270 |
| boza, a natural source of probiotic lactic acid bacteria. | to evaluate the probiotic properties of strains isolated from boza, a traditional beverage produced from cereals. | 2008 | 17922827 |
| effects of lactobacillus rhamnosus gg on the cell growth and polyamine metabolism in hgc-27 human gastric cancer cells. | previous in vivo studies have suggested that lactobacilli can exert anti-proliferative effects on the gastric epithelium. however, few data are available on their mechanisms of action. the aim of this study was to investigate the effects of increasing concentrations of lactobacillus rhamnosus strain gg (l. gg) homogenate on cell growth and proliferation [by 3-(4,5 di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, [(3)h]-thymidine incorporation and polyamine biosynthesis] and apoptosis proc ... | 2007 | 17927509 |
| lactobacillus rhamnosus as additive for maize and sorghum ensiling. | the effects of lactobacillus rhamnosus at195, a potential probiotic microorganism cultured in buffalo "scotta" whey, on chemical and microbiological composition in maize and sorghum ensiling were evaluated. both crops were harvested, chopped, and treated or not with the selected strain prior to ensiling in fiberglass vertical silos; 90 days after ensiling, silages were sensorially evaluated and sampled. different chemical components were evaluated both on fresh crops and silages: in particular, ... | 2007 | 17929890 |
| pediatric applications of inulin and oligofructose. | inulin-type fructans have been used in infants and children because of their prebiotic potential to modulate the intestinal flora and influence the innate and adaptive immune response favorably. a mixture of long chain inulin (5-60 monomers) in combination with galactooligosaccharides (gos) (2-7 monomers) has been added to infant formula in europe in a 10-90% ratio for over 5 y. clinical studies have demonstrated that these prebiotic formulas have significant effects on flora composition, improv ... | 2007 | 17951508 |
| characterization of dominant lactic acid bacteria isolated from são jorge cheese, using biochemical and ribotyping methods. | to identify, using phenotypic and genotypic methods, the dominant lactic acid bacteria (lab) present in são jorge cheese - one of the 11 portuguese cheeses currently bearing an appéllation d'origine protegée status. | 2007 | 17953594 |
| lactobacillus rhamnosus r11 consumed in a food supplement survived human digestive transit without modifying microbiota equilibrium as assessed by real-time polymerase chain reaction. | the aim of this study was to evaluate the survival of lactobacillus rhamnosus r11 and lactobacillus acidophilus r52 in the human digestive tract and their effects on the microbiota homeostasis. we designed an open human trial including 14 healthy volunteers. a 3-week exclusion period of fermented products was followed by a 12-day consumption period of 4 capsules daily containing 2 x 10(9)l. rhamnosus r11 and 1 x 10(8)l. acidophilus r52, and a 12-day wash-out period. the 2 strains and dominant ba ... | 2008 | 17957115 |
| effect of a milk formula containing probiotics on the fecal microbiota of asian infants at risk of atopic diseases. | the fecal microbiota of 37 infants with (n = 20) or without (n = 17) probiotic administration was evaluated on d 3, and at 1, 3, and 12 mo by fluorescence in situ hybridization-flow cytometry (fish-fc), pcr, and bacteriological culture methods. they represent consecutive subjects of an ongoing double-blind, placebo-controlled trial on a probiotic formula (lgg and bifidobacterium longum) administered during the first 6 mo of life. despite varying composition in each baby, there was a general bact ... | 2007 | 17957155 |
| the effect of probiotics on respiratory infections and gastrointestinal symptoms during training in marathon runners. | heavy exercise is associated with an increased risk of upper respiratory tract infections. strenuous exercise also causes gastrointestinal (gi) symptoms. in previous studies probiotics have reduced respiratory tract infections and gi symptoms in general populations including children, adults, and the elderly. these questions have not been studied in athletes before. the purpose of this study was to investigate the effect of probiotics on the number of healthy days, respiratory infections, and gi ... | 2007 | 17962710 |
| [administration of probiotic lactobacilli to children with gastrointestinal problems: there is still little evidence]. | probiotics are defined as 'live micro-organisms that confer a health benefit on the host if administered in adequate amounts'. there are only limited data in the literature regarding the efficacy and safety of lactobacilli for various gastrointestinal indications in children. lactobacillus rhamnosus gg (lgg) has no added value in the treatment of children with functional constipation. lactobacilli do seem to be effective in rotavirus diarrhoea. they seem safe and shorten the duration of the diar ... | 2007 | 17969569 |
| heat- and formalin-inactivated probiotic bacteria induce comparable cytokine patterns in intestinal epithelial cell-leucocyte cocultures. | the mode of inactivation of probiotic bacteria may profoundly affect their immune-modulatory properties to the point of reversal of effects in in vitro human intestinal epithelial-like cell cultures (caco-2). to further investigate the influence of inactivation treatment on cytokine production, three probiotic strains were evaluated-live, heat-inactivated, and formalin-inactivated strains-for their impact on interleukin (il) 6, il-8, and il-10 production in caco-2-leucocyte cocultures. the teste ... | 2007 | 17969629 |
| study on the protective role of selenium against cadmium toxicity in lactic acid bacteria: an advanced application of icp-ms. | in this work, lactobacillus casei rhamnosus were obtained from the commercial product of fermented milk and possible antagonistic effect of selenium (as sodium selenite) against cadmium toxicity was studied. the bacteria capability to incorporate se was demonstrated: after 1 week exposure to se(iv), its total concentration in the freeze-dried biomass was 405+/-28 microg/g (7.4+/-0.8 microg/g in control). in the presence of se(iv) and cd(ii), the bacterial growth and cell viability were improved ... | 2008 | 17977654 |
| evaluation of the response of lactobacillus rhamnosus vtt e-97800 to sucrose-induced osmotic stress. | environmental osmotic changes are one of the stresses live probiotics may encounter either in their natural habitats or as a result of usage in food formulations and processing. response to osmotic stress, induced by sucrose, of the probiotic strain lactobacillus rhamnosus vtt e-97800 (e800) was investigated. the fluorescence-based approach used, by combined staining with caboxyfluorescein (cfda) and propidium iodide (pi) could give insights on the osmotic-induced changes of microbial esterase a ... | 2008 | 17993393 |