| effect of soy isoflavones on growth of representative bacterial species from the human gut. | the present work aimed to assess the susceptibility of dominant and representative bacterial populations from the human gut to isoflavones and their metabolites. to do so, the minimum inhibitory concentration (mic) of isoflavone glycosides, isoflavone aglycones, and equol to 37 bacterial strains was determined by broth microdilution. additionally, for 10 representative strains, growth curves, growth rate (μ), and optical density (od600 nm) of the cultures at 24 h were also determined. mics of da ... | 2017 | 28698467 |
| early gut mycobiota and mother-offspring transfer. | the fungi in the gastrointestinal tract, the gut mycobiota, are now recognised as a significant part of the gut microbiota, and they may be important to human health. in contrast to the adult gut mycobiota, the establishment of the early gut mycobiota has never been described, and there is little knowledge about the fungal transfer from mother to offspring. | 2017 | 28837002 |
| administration of two probiotic strains during early childhood does not affect the endogenous gut microbiota composition despite probiotic proliferation. | probiotics are increasingly applied to prevent and treat a range of infectious, immune related and gastrointestinal diseases. despite this, the mechanisms behind the putative effects of probiotics are poorly understood. one of the suggested modes of probiotic action is modulation of the endogenous gut microbiota, however probiotic intervention studies in adults have failed to show significant effects on gut microbiota composition. the gut microbiota of young children is known to be unstable and ... | 2017 | 28818050 |
| the impact of storage conditions on the stability of lactobacillus rhamnosus gg and bifidobacterium animalis subsp. lactis bb12 in human milk. | human milk is the optimal source of complete nutrition for neonates and it also guides the development of infant gut microbiota. importantly, human milk can be supplemented with probiotics to complement the health benefits of breastfeeding. storage of human milk for limited periods of time is often unavoidable, but little is known about the effect of different storage conditions (temperature) on the viability of the added probiotics. therefore, in this study, we evaluated how different storage c ... | 2017 | 28753043 |
| probiotics and child care absence due to infections: a randomized controlled trial. | the risk of infections is higher in children attending child care compared with children cared for at home. this study examined the effect of a combination of probiotics on absence from child care because of respiratory and gastrointestinal infections in healthy infants aged 8 to 14 months at the time of enrollment in child care. | 2017 | 28674113 |
| efficacy and safety of probiotic-supplemented triple therapy for eradication of helicobacter pylori in children: a systematic review and network meta-analysis. | the aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with helicobacter pylori infection. | 2017 | 28681177 |
| the potential synergistic behaviour of inter- and intra-genus probiotic combinations in the pattern and rate of short chain fatty acids formation during fibre fermentation. | this study compared the rate of short chain fatty acid (scfa) production by different probiotic combinations of lactobacillus and bifidobacterium to determine any synergistic effects. six different fibre fractions were fermented with nine combinations of lactobacillus rhamnosus (lr), lactobacillus acidophilus (la), bifidobacterium longum (bl) and bifidobacterium breve (bb) for 0, 6, 24 and 48 h. scfas were quantified by gas chromatography. inter-genus combinations of bacteria produced more scfa, ... | 2017 | 28659066 |
| adamdec1, ednrb and ptgs1/cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains. | we have previously reported that administration of lactobacillus paracasei cncm i-4034, bifidobacterium breve cncm i-4035 and lactobacillus rhamnosus cncm i-4036 to obese zucker-lepr (fa/fa) rats attenuates liver steatosis and exerts anti-inflammatory effects. the goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese zucker-lepr (fa/fa) rats fed the probiotic strains using a dna microarray and postgenomic techniques. we also measured ... | 2017 | 28512356 |
| to add or not to add probiotics to infant formulae? an updated systematic review. | we updated evidence on the effects of the administration of probiotic-supplemented infant formulae (if) compared with unsupplemented if. five databases were searched up to september 2016 for randomised controlled trials. twenty publications were identified, including five new rcts. supplementation of if with bifidobacterium lactis bb12, either alone or with streptococcus thermophilus, had no effect on growth, respiratory illness, antibiotic use, stool frequency or consistency. however, there was ... | 2017 | 28856907 |
| effect of lactobacillus rhamnosus hn001 and bifidobacterium longum bb536 on the healthy gut microbiota composition at phyla and species level: a preliminary study. | to evaluate the ability of lactobacillus rhamnosus hn001 and bifidobacterium longum bb536 to colonize the intestinal environment of healthy subjects and modify the gut microbiota composition. | 2017 | 28487606 |
| effects of hot-water extracts from ganoderma lucidum residues and solid-state fermentation residues on prebiotic and immune-stimulatory activities in vitro and the powdered residues used as broiler feed additives in vivo. | large amounts of ganoderma lucidum (gl) commercial products are provided in the worldwide market such as powders, tea bags, or capsules as dietary supplements which contained triterpenoids and/or polysaccharides. therefore, it was estimated that several thousand tons of gl residues (glr) are produced and discarded. for recycling uses, the aim of this study was to evaluate the benefits of two hot-water extracts from glr (hwp_glr) and solid-state fermentation glr inoculated with gl mycelia (hwp_gl ... | 2015 | 28510826 |
| probiotic yogurt and acidified milk similarly reduce postprandial inflammation and both alter the gut microbiota of healthy, young men. | probiotic yogurt and milk supplemented with probiotics have been investigated for their role in 'low-grade' inflammation but evidence for their efficacy is inconclusive. this study explores the impact of probiotic yogurt on metabolic and inflammatory biomarkers, with a parallel study of gut microbiota dynamics. the randomised cross-over study was conducted in fourteen healthy, young men to test probiotic yogurt compared with milk acidified with 2 % d-(+)-glucono-δ-lactone during a 2-week interve ... | 2017 | 28558854 |
| probiotics and antibiotic-associated diarrhea in children: a review and new evidence on lactobacillus rhamnosus gg during and after antibiotic treatment. | antibiotic associated diarrhea (aad) is a common complication in childhood in the outpatient and inpatient settings. this review provides up to date information on the use of probiotics in the prevention and treatment of aad, including that from clostridium difficile, in children. the most recently systematic reviews and subsequently published randomized controlleds trials are considered. different single and multistrain probiotics are described; a specific recommendation for the use of lactobac ... | 2017 | 28827186 |
| the individual contribution of starter and non-starter lactic acid bacteria to the volatile organic compound composition of caciocavallo palermitano cheese. | the contribution of two starter (lactobacillus delbrueckii and streptococcus thermophilus) and nine non-starter (enterococcus casselliflavus, enterococcus faecalis, enterococcus durans, enterococcus gallinarum, lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, pediococcus acidilactici and pediococcus pentosaceus) species of lactic acid bacteria (lab) to the volatile organic compounds (vocs) of caciocavallo palermitano cheese was investigated. the strains used in this study w ... | 2017 | 28783535 |
| screening of oxalate degrading lactic acid bacteria of food origin. | a screening for oxalate degrading abilities was initially carried on within lactic acid bacteria cultures of different food origin. seventy-nine strains were drop-inoculated onto mrs agar plates containing calcium oxalate. by comparing colonies diameters, 31 strains were used to inoculate, in parallel, mrs and mrs modified by sodium oxalate addition. differences in the strains' growth were assessed by colony forming unit counts. for two strains, the growth in oxalate enriched medium was signific ... | 2017 | 28713789 |
| testing commercial biopreservative against spoilage microorganisms in map packed ricotta fresca cheese. | ricotta fresca cheese is susceptible to secondary contamination and is able to support the growth of pathogens or spoilage psychotrophic bacteria during storage. the aim of the present study was to evaluate which among three commercial biopreservatives was suitable to be used to control the growth of spoilage microorganisms in sheep's milk map ricotta fresca cheese. 144 ricotta fresca cheese samples were inoculated either with the bioprotective culture lyofast fpr 2 (including enterococcus faeci ... | 2017 | 28576375 |
| short communication: a study of lactobacillus isolates' adherence to and influence on membrane integrity of human caco-2 cells. | the selection criteria of ideal probiotic bacteria are complex and involve many factors. one key criterion is based on the ability of the probiotic bacteria to adhere to the epithelial lining of the gastrointestinal tract. the objective of this study was to evaluate and compare the adherence and influence on membrane integrity of 2 selected lactobacilli isolates-lactobacillus rhamnosus mi13 (dairy food origin) and l. plantarum rc2 (bovine rumen origin)-to caco-2 cells in the presence and absence ... | 2017 | 28822542 |
| e. coli o124 k72 alters the intestinal barrier and the tight junctions proteins of guinea pig intestine. | our research group previously isolated and identified a strain of pathogenic escherichia coli from clinical samples called e. coli o124 k72. the present study was aimed at determining the potential effects of e. coli o124 k72 on intestinal barrier functions and structural proteins integrity in guinea pig. guinea pigs were grouped into three groups; control (cg); e. coli o124 k72 (e. coli); and probiotics lactobacillus rhamnosus (lgg). initially, we create intestinal dysbiosis by giving all anima ... | 2017 | 28779708 |
| probiotic lactobacillus strains stimulate the inflammatory response and activate human macrophages. | lactobacilli have been shown to promote health functions. in this study, we analyzed the mechanism by which four different strains of probiotics affected innate immunity, such as regulation of ros, cytokines, phagocytosis, bactericidal activity, signaling by nf-κb pp65, and tlr2 activation. the production of ros was dependent on the concentration and species of lactobacillus. the results obtained from the tested strains (lactobacillus rhamnosus gg, l. rhamnosus klsd, l. helveticus imau70129, and ... | 2017 | 28758133 |
| arabinoxylans, inulin and lactobacillus reuteri 1063 repress the adherent-invasive escherichia coli from mucus in a mucosa-comprising gut model. | the microbiota that colonises the intestinal mucus may particularly affect human health given its proximity to the epithelium. for instance, the presence of the adherent-invasive escherichia coli (aiec) in this mucosal microbiota has been correlated with crohn's disease. using short-term screening assays and a novel long-term dynamic gut model, which comprises a simulated mucosal environment (m-shime), we investigated how (potential) pro- and prebiotics may repress colonisation of aiec from mucu ... | 2016 | 28721250 |
| crystallization and x-ray diffraction analysis of spae, a basal pilus protein from the gut-adapted lactobacillus rhamnosus gg. | spae is the predicted basal pilin subunit in the sortase-dependent spafed pilus from the gut-adapted and commensal lactobacillus rhamnosus gg. thus far, structural characterization of the cell-wall-anchoring basal pilins has remained difficult and has been limited to only a few examples from pathogenic genera and species. to gain a further structural understanding of the molecular mechanisms that are involved in the anchoring and assembly of sortase-dependent pili in less harmful bacteria, l. rh ... | 2017 | 28580919 |
| effects of intravaginal lactic acid bacteria on bovine endometrium: implications in uterine health. | infection and inflammation of the endometrium after calving compromise uterine health, contributing to decreased reproductive efficiency in dairy cows. twenty multiparous cows were distributed in two groups and treated intra-vaginally with a combination of lactic acid bacteria (lab) composed by lactobacillus rhamnosus, pedioccocus acidilactici, and lactobacillus reuteri, or with a sterile carrier (con) twice per week during 3 wk. at the slaughterhouse, vaginal and endometrial swabs were taken fo ... | 2017 | 28532798 |
| [lactobacillus rhamnosus gg improves symptoms and its mechnism in mice with ovalbumin-induced food allergy]. | objective to investigate the effect of lactobacillus rhamnosus gg (lgg) on peripheral blood il-4/ifn-γ in mice with ovalbumin-induced food allergy. methods the mice with food allergy were divided into 3 groups (n=10 in each group): food allergy model group, low-dose lgg group (1×10(8) cfu/ml, 200 μl/d) and high-dose lgg group (1×10(9) cfu/ml, 200 μl/d). the healthy mice (n=10) served as a control group. mice in the lgg groups were given lgg for consecutive 22 days after modeling. the control mic ... | 2017 | 28502295 |
| targeting virulence: salmochelin modification tunes the antibacterial activity spectrum of β-lactams for pathogen-selective killing of escherichia coli. | new antibiotics are required to treat bacterial infections and counteract the emergence of antibiotic resistance. pathogen-specific antibiotics have several advantages over broad-spectrum drugs, which include minimal perturbation to the commensal microbiota. we present a strategy for targeting antibiotics to bacterial pathogens that utilises the salmochelin-mediated iron uptake machinery of gram-negative escherichia coli. salmochelins are c-glucosylated derivatives of the siderophore enterobacti ... | 2015 | 28717471 |
| screening of lactic acid bacteria for anti-fusarium activity and optimization of incubation conditions. | anti-fusarium activities of lactic acid bacteria (lab) lactobacillus plantarum 299v, l. plantarum nrrl-4496, and lactobacillus rhamnosus vt1 were determined by a microdilution assay developed in this study against fusarium graminearum 08/rg/bf/51. a cell-free lactobacillus culture supernatant (cflcs) of l. rhamnosus vt1 had the highest anti-fusarium activity. response surface methodology was used to optimize the incubation conditions for production of cflcs. a box-behnken factorial design was us ... | 2017 | 28853625 |
| correction: jang, s.-e.; et al. lactobacillus rhamnosus hn001 and lactobacillus acidophilus la-14 attenuate gardnerella vaginalis-infected bacterial vaginosis in mice. nutrients 2017, 9, 531. | we would like to submit the following corrections to our recently published paper [1] due to the reported wrong name of probiotic mixture and the dose of some drugs. ...]. | 2017 | 28686189 |
| lactobacillus rhamnosus hn001 and lactobacillus acidophilus la-14 attenuate gardnerella vaginalis-infected bacterial vaginosis in mice. | oral administration of a probiotic mixture (pm; respecta(®)) consisting of lactobacillus rhamnosus hn001 (l1), lactobacillus acidophilus la-14 (l2), and lactoferrin rcxtm results in colonization of these probiotics in the vagina of healthy women. therefore, we examined whether vaginal colonization of the pm ingredients l1 and l2 could attenuate bacterial vaginosis (bv). bv was induced in mice via β-estradiol-3-benzoate-induced immunosuppression and intravaginal inoculation with gardnerella vagin ... | 2017 | 28545241 |
| immunomodulatory effects and anti-candida activity of lactobacilli in macrophages and in invertebrate model of galleria mellonella. | due to the growing number of multi-resistant candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. this study evaluated the immunomodulation and anti-candida activity of lactobacillus rhamnosus (lr), lactobacillus acidophilus and lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (raw 264.7) and galleria mellonella (gm). mouse macrophages were activated by different lactobacilli suspensions and challenged ... | 2017 | 28801270 |
| effect of lactobacillus on body weight and body fat in overweight subjects: a systematic review of randomized controlled clinical trials. | gut microbiota is important for maintaining body weight. modulation of gut microbiota by probiotics may result in weight loss and thus help in obesity treatment. the aim of this systematic review was to evaluate the effects of lactobacillus on weight loss and/or fat mass in overweight adults. a search was performed on the medline (pubmed) and scopus electronic databases using the search terms: 'probiotics', 'lactobacillus, 'obesity', 'body weight changes', 'weight loss', 'overweight', 'abdominal ... | 2017 | 28792488 |
| effect of eudragit s100 nanoparticles and alginate chitosan encapsulation on the viability of lactobacillus acidophilus and lactobacillus rhamnosus. | in this study, we examined a novel method of microencapsulation with calcium alginate-chitosan and eudragit s100 nanoparticles for the improving viability of probiotic bacteria, lactobacillus acidophilus and lactobacillus rhamnosus. extrusion technique was carried out in microencapsulation process. the viability of two probiotics in single coated beads (with only chitosan), double coated beads (with chitosan and eudragit nanoparticles), and as free cells (unencapsulated) were conducted in simula ... | 2017 | 28687035 |
| the role of probiotics in children with autism spectrum disorder: a prospective, open-label study. | there are limited data on the efficacy of probiotics in children with asd, therefore, this study aims to evaluate the efficacy and tolerability of probiotics in an egyptian cohort of children with asd. | 2017 | 28686541 |
| effects of 4 probiotic strains in coculture with traditional starters on the flavor profile of yogurt. | to study the influence of probiotics on the flavor profile of yogurt, 4 probiotics, including lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus casei, were cofermented with traditional starters. the changes of bacterial growth, acid contents and volatile compounds of yogurt were investigated during fermentation and refrigerated storage. the strains that exhibited a low growth rate in milk did not significantly affect the bacterial population dynamics, ... | 2017 | 28598540 |
| aggregation and adhesion activity of lactobacilli isolated from fermented products in vitro and in vivo: a potential probiotic strain. | approximately 25 strains of lactobacilli isolated from different dairy products and fermented vegetables were screened according to their possibility to show the high auto-aggregation and co-aggregation. the strains lactobacillus helveticus inra-2010-h11, lactobacillus rhamnosus ina-5.1, and lactobacillus acidophilus jm-2012 were determined to have the high auto-aggregation (approximately 73, 46, and 70.5% correspondingly). a high co-aggregation capacity (75.53%) for strains inra-2010-h11 and jm ... | 2017 | 28455689 |
| probiotics to prevent necrotising enterocolitis and nosocomial infection in very low birth weight preterm infants. | the aim of the study was to determine whether routine probiotic supplementation (rps) with lactobacillus rhamnosus gg (lgg) or lactobacillus acidophilus +lactobacillus bifidum is associated with reduced risk of necrotising enterocolitis (nec)≥stage ii in preterm neonates born at ≤32 weeks' gestation. we conducted a retrospective cohort study on the effect of probiotic supplementation in very low birth weight infants in our neonatal unit by comparing two periods: before and after supplementation. ... | 2017 | 28443531 |
| metabolic gene-targeted monitoring of non-starter lactic acid bacteria during cheese ripening. | long ripened cheeses, such as grana padano (gp), a protected designation of origin (pdo) italian cheese, harbor a viable microbiota mainly composed of non-starter lactic acid bacteria (nslab), which contribute to the final characteristics of cheese. the nslab species lactobacillus rhamnosus, lb. casei and lb. paracasei are frequently found in gp, and form a closely related taxonomic group (lb. casei group), making it difficult to distinguish the three species through 16s rrna sequencing. spxb, a ... | 2017 | 28735145 |
| prophylactic effects of secretion metabolites of dairy lactobacilli through downregulation of erbb-2 and erbb-3 genes on colon cancer cells. | colon cancer is one of the most prevalent cancers, and intestinal microbial community plays a pivotal role in colorectal tumor genesis. probiotics as live microorganisms may be able to exert an anticancer effect in colon cancer. the aim of this study was to isolate and identify lactobacillus spp. from traditional dairy products with probiotic properties and to investigate their anticancer effects through erbb-2 and erbb-3 gene expression in colon cancer cells. the isolated lactobacilli from yogu ... | 2017 | 28683007 |
| in silico prediction, in vitro antibacterial spectrum, and physicochemical properties of a putative bacteriocin produced by lactobacillus rhamnosus strain l156.4. | a bacteriocinogenic lactobacillus rhamnosus l156.4 strain isolated from the feces of nih mice was identified by 16s rrna gene sequencing and maldi-tof mass spectrometry. the entire genome was sequenced using illumina, annotated in the pgaap, and rast servers, and deposited. conserved genes associated with bacteriocin synthesis were predicted using bagel3, leading to the identification of an open reading frame (orf) that shows homology with the l. rhamnosus gg (atcc 53103) prebacteriocin gene. th ... | 2017 | 28579977 |
| study on the chemical and microbial composition and probiotic characteristics of dominant lactic acid bacteria in fermented poultry slaughterhouse waste. | this study aimed to evaluate the quality of fermented poultry slaughterhouse waste and isolate and identify the probiotic bacteria present in it. poultry slaughterhouse waste samples supplemented with 5% starter culture and 0.2% propionic acid received one of four experimental treatments, which consisted of further supplementation with: (1) 10% molasses (m10); (2) 15% molasses (m15); (3) 10% molasses with 200ppm butylated hydroxytoluene (mb10); and (4) 15% molasses with 200ppm butylated hydroxyt ... | 2017 | 28408278 |
| supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (sander lucioperca) reared in recirculating aquaculture system (ras): a pilot study. | this research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed otohime fish diet, or through artemia nauplii, on fish growth, microbiota balance and skeletal development. on the 12th day post hatching (dph) fish were divided into following treatment groups: two groups received the combination of otohime and nauplii enriched either with lactobacillus paracasei bghn14+lactobacillus rhamnosus bgt10 or with lactobacillus reuteri bggo6-55+lactobacil ... | 2017 | 28777955 |
| survival of potential probiotic lactobacilli used as adjunct cultures on pecorino siciliano cheese ripening and passage through the gastrointestinal tract of healthy volunteers. | in the present study, two lactobacilli strains, lactobacillus rhamnosus h25 and lactobacillus paracasei n24, used as adjunct cultures, were evaluated for their heat resistance both with and without prior heat adaptation and for their survival, at industrial scale, during the production and ripening of the pecorino siciliano cheese. in addition, the viability and persistence of the lactobacilli strains after passage through the gastrointestinal tract of healthy volunteers were evaluated by using ... | 2017 | 28458191 |
| dietary fibers and protective lactobacilli drive burrata cheese microbiome. | this study aimed at improving the functional attributes and shelf-life of burrata cheese by using protective lactobacilli (lactobacillus plantarum lpal and lactobacillus rhamnosus lrb), fructoligosaccharides and inulin. six burrata cheeses were made by using: i) the traditional protocol (control); ii) addition of 0.5% fructoligosaccharides and inulin (df cheese); iii) protective lactobacilli in milk alone (pl cheese); iv) protective lactobacilli in milk and governing liquid (2pl cheese); v) prot ... | 2017 | 28842539 |
| milk fermented by lactobacillus species from brazilian artisanal cheese protect germ-free-mice against salmonella typhimurium infection. | ingestion of milks fermented by lactobacillus strains showing probiotic properties is an important tool to maintain gastrointestinal health. in this study, lactobacillus rhamnosus d1 and lactobacillus plantarum b7, isolated from brazilian artisanal cheese, were used as starters for the functional fermented milks to assess their probiotic properties in a gnotobiotic animal model. male germ-free swiss mice received a single oral dose of milk fermented by each sample, and were challenged with salmo ... | 2017 | 28789560 |
| efficacy of lactobacillus reuteri dsm 17938 for the treatment of acute gastroenteritis in children: protocol of a randomized controlled trial. | acute gastroenteritis (age) is one of the most common diseases among children. oral rehydration therapy is the key treatment. however, despite proven efficacy, it remains underused. this is because oral rehydration solution neither reduces the frequency of bowel movements and fluid loss nor shortens the duration of illness. hence, there is interest in adjunctive treatments. according to the 2014 guidelines developed by the european society for paediatric gastroenterology, hepatology and nutritio ... | 2017 | 28835355 |
| aciduric strains of lactobacillus reuteri and lactobacillus rhamnosus, isolated from human feces, have strong adhesion and aggregation properties. | human feces were streaked onto mrs agar adjusted to ph 2.5, 3.0, and 6.4, respectively, and medium supplemented with 1.0% (w/v) bile salts. two aciduric strains, identified as lactobacillus reuteri hfi-ld5 and lactobacillus rhamnosus hfi-k2 (based on 16s rdna and reca sequences), were non-hemolytic and did not hydrolyze mucin. the surface of lactobacillus reuteri hfi-ld5 cells has a weak negative charge, whereas lactobacillus rhamnosus hfi-k2 has acidic and basic properties, and produces exopoly ... | 2017 | 28756502 |
| peptidoglycan from immunobiotic lactobacillus rhamnosus improves resistance of infant mice to respiratory syncytial viral infection and secondary pneumococcal pneumonia. | several research works have demonstrated that beneficial microbes with the capacity to modulate the mucosal immune system (immunobiotics) are an interesting alternative to improve the outcome of bacterial and viral respiratory infections. among the immunobiotic strains with the capacity to beneficially modulate respiratory immunity, lactobacillus rhamnosus crl1505 has outstanding properties. although we have significantly advanced in demonstrating the capacity of l. rhamnosus crl1505 to improve ... | 2017 | 28848552 |
| a comparative study of procedures for binding of aflatoxin m1 to lactobacillus rhamnosus gg. | several strains of lactic acid bacteria (lab), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. this study was carried out to investigate the effect of different concentrations of lactobacillus rhamnosus gg (atcc 53103) to bind aflatoxin m1 (afm1) in liquid media. afm1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifu ... | 2017 | 28843807 |
| study of the effects of spray-drying in whey-starch on the probiotic capacity of lactobacillus rhamnosus 64 in the gut of mice. | to evaluate the effects of spray-drying of lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of tnbs-induced colitis in mice. | 2017 | 28815811 |
| effect of fermented milk containing lactobacillus rhamnosus sd11 on oral microbiota of healthy volunteers: a randomized clinical trial. | the aims of this study were to evaluate whether short-term consumption of fermented milk containing lactobacillus rhamnosus sd11 affected levels of oral microbiota in vivo and whether l. rhamnosus sd11 could colonize in the human mouth. we also monitored for potential side effects of the probiotic. the applicability of using l. rhamnosus sd11 compared with lactobacillus bulgaricus as a starter culture for fermented milk was evaluated. after informed consent, 43 healthy young adults were recruite ... | 2017 | 28803012 |
| early probiotic supplementation for eczema and asthma prevention: a randomized controlled trial. | to determine if probiotic administration during the first 6 months of life decreases childhood asthma and eczema. | 2017 | 28784701 |
| interplay between lactobacillus rhamnosus gg and candida and the involvement of exopolysaccharides. | a number of clinical studies have shown protective effects of lactobacilli against candida species in the gastrointestinal tract, the urogenital tract and the oral cavity, while others did not show clear effects. evidence on the mode of action of lactobacilli against candida is also still lacking. in this study, the anti-candida activity of the model probiotic strain lactobacillus rhamnosus gg was explored in different assays to determine molecular interactions. we found that l. rhamnosus gg was ... | 2017 | 28772020 |
| oral administration of lactobacillus rhamnosus gg ameliorates salmonella infantis-induced inflammation in a pig model via activation of the il-22bp/il-22/stat3 pathway. | the high rate of salmonella enterica serovar infantis (s. infantis) infection poses significant risk for the development of non-typhoidal salmonella gastroenteritis. however, efficient strategies to prevent or treat the infection remain elusive. here, we explored the effect of the probiotic lactobacillus rhamnosus gg (lgg) administration in preventing s. infantis infection in a pig model. probiotic lgg (1.0 × 10(10) cfu/day) was orally administered to newly weaned piglets for 1 week before s. in ... | 2017 | 28770173 |
| systematic review: probiotics for functional constipation in children. | we updated our 2010 systematic review on the efficacy of probiotics in the treatment of constipation in children. the medline, embase, and cochrane library databases; clinical trial registries; and reference lists of included studies were searched to february 2017 for randomized controlled trials (rcts) performed in children, with no language restriction. the primary outcome measure was treatment success, as defined by the investigators. we included seven rcts with a total of 515 participants. i ... | 2017 | 28762070 |
| influence of commercial inactivated yeast derivatives on the survival of probiotic bacterium lactobacillus rhamnosus hn001 in an acidic environment. | this study evaluated the influence of three inactivated yeast derivatives (iyds) used in wine production, namely optired(®), optiwhite(®) and noblesse(®), on the viability of the probiotic strain lactobacillus rhamnosus hn001 in an acidic environment. addition of the iyds at 3 g/l significantly enhanced the survival of the probiotic bacteria by 2.75-4.05 log cycles after 10-h exposure in a ph 3.0 buffer. acid stress assay with iyd components obtained after centrifugation and filtration revealed ... | 2017 | 28747041 |
| novel molecular insights about lactobacillar sortase-dependent piliation. | one of the more conspicuous structural features that punctuate the outer cell surface of certain bacterial gram-positive genera and species is the sortase-dependent pilus. as these adhesive and variable-length protrusions jut outward from the cell, they provide a physically expedient and useful means for the initial contact between a bacterium and its ecological milieu. the sortase-dependent pilus displays an elongated macromolecular architecture consisting of two to three types of monomeric pro ... | 2017 | 28718795 |
| dietary lipid content reorganizes gut microbiota and probiotic l. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish. | in the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. diets containing three different lipid levels (high [hfd], medium [mfd], and low [lfd]) were administered with or without the supplementation of lactobacillus rhamnosus (p) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. dietary lipid content shifted the gut microbiome structure. the addition of l. rhamnosus in the diets, induced trans ... | 2017 | 28717234 |
| clinical and microbiological aspects of the use of lactobacillus rhamnosus pl1 strains in proctological patients with symptoms of chronic proctitis. | recurrent proctitis and the symptoms associated therewith pose significant clinical problem in proctological patients. the objective of this study was to assess the impact of the probiotic lactobacillus rhamnosus pl1 strain on the clinical presentation and composition of intestinal microbiota in patients with symptoms of proctitis in the course of hemorrhoidal disease and diverticulosis. material consisted of 24 patients in whom no complete clinical improvement could be obtained after the treatm ... | 2017 | 28703111 |
| correction: genotypic and phenotypic diversity of lactobacillus rhamnosus clinical isolates, their comparison with strain gg and their recognition by complement system. | [this corrects the article doi: 10.1371/journal.pone.0176739.]. | 2017 | 28700750 |
| purification and fermentation characteristics of exopolysaccharide from fomitopsis castaneus imaz. | short-chain fatty acids (scfas), which are the end products of carbohydrate fermentation in the gut, mainly contribute to energy metabolism in mammals. the amount of scfas produced during fermentation is an important parameter that characterizes the fermentation capacity of a system. this paper reports on the fermentation characteristics of exopolysaccharides (eps) isolated from fomitopsis castaneus imaz, a wood-rot fungal species. we isolated and purified the main eps fraction by freeze drying ... | 2017 | 28698079 |
| lactobacillus rhamnosus endocarditis after upper endoscopy. | lactobacillus rhamnosus is commonly found in gastrointestinal flora and used in probiotics but is a rare human pathogen. we report a case of l. rhamnosus endocarditis following upper endoscopy in a frequent consumer of yogurt containing the organism, who required aortic and mitral valve replacement for cure. | 2017 | 28695143 |
| complete genome sequence of lactobacillus rhamnosus bfe5264, isolated from maasai traditional fermented milk. | here, we report the complete genome of lactobacillus rhamnosus bfe5264, which was sequenced with the pacific biosciences rsii platform. the genome size is 3.01 mb and includes 3,077 annotated coding sequences, including genes associated with the promotion of intestinal epithelial homeostasis through specific signaling pathways. | 2017 | 28684572 |
| a casein hydrolysate based formulation attenuates obesity and associated non-alcoholic fatty liver disease and atherosclerosis in ldlr-/-.leiden mice. | obesity frequently associates with the development of non-alcoholic fatty liver disease (nafld) and atherosclerosis. chronic inflammation in white adipose tissue (wat) seems to be an important driver of these manifestations. | 2017 | 28678821 |
| effects of lactobacillus rhamnosus gg on the maturation and differentiation of dendritic cells in rotavirus-infected mice. | rotavirus-related diarrhoea is considered one of the most important diseases in field animal production. in addition to the classic vaccine strategy, a number of studies have utilised probiotics, such as lactobacillus rhamnosus gg (lgg), to prevent rotavirus-induced diarrhoea. although it has been partially revealed that toll-like receptors (tlrs) are involved in the lgg-mediated protection against rotavirus infection, the details of the underlying immunologic mechanisms remain largely unknown. ... | 2017 | 28670908 |
| pre-cultivation with selected prebiotics enhances the survival and the stress response of lactobacillus rhamnosus strains in simulated gastrointestinal transit. | in our study, we dwelled upon combinations of lactobacilli/prebiotics, considering four different strains belonging to the lactobacillus rhamnosus species, including lactobacillus rhamnosus gg (lgg), and different prebiotics often found in commercial synbiotic products, such as inulin, lactulose and polyols mannitol and sorbitol. in the first step of the research, the survival, the growth kinetic parameters and the protein expression of lb. rhamnosus strains cultivated in presence of the differe ... | 2017 | 28659890 |
| the inhibitory impacts of lactobacillus rhamnosus gg-derived extracellular vesicles on the growth of hepatic cancer cells. | bacterial extracellular vesicles (evs) have come forth into notice as possible important agent to mediate host-pathogen interactions. in this scientific research, the authors have tried to find out the effect of evs derived from lactobacillus rhamnosus gg (ldevs) on the apoptosis induction in hepg2 cell line. the evs were purified from the conditioned medium of lactobacillus rhamnosus gg using ultrafiltration and confirmed by transmission electron microscopy (tem). the hepg2 cells were treated w ... | 2017 | 28634130 |
| lactobacillus rhamnosusgg reduces hepatic fibrosis in a model of chronic liver disease in rats | the intestinal dysbiosis is common in chronic liver disease and can induce to inflammatory responses and mediate the collagen deposition in the liver. | 2017 | 28627210 |
| zearalenone adsorption capacity of lactic acid bacteria isolated from pigs. | the ability to adsorb zearalenone by five strain of lactic acid bacteria was evaluated: four strains of lactobacillus spp. isolated from pig rectal swabs and one commercial strain (lactobacillus rhamnosus). several factors affecting the adsorption capacity were evaluated in order to improve the adsorption of the mycotoxin by bacteria. the stability of the zearalenone-bacteria complex was analyzed. in every case, bacterial adsorption capacity was higher than 40.0%. the strain showing the highest ... | 2017 | 28623104 |
| inorganic salts and intracellular polyphosphate inclusions play a role in the thermotolerance of the immunobiotic lactobacillus rhamnosus crl 1505. | in this work, the thermotolerance of lactobacillus rhamnosus crl1505, an immunobiotic strain, was studied as a way to improve the tolerance of the strain to industrial processes involving heat stress. the strain displayed a high intrinsic thermotolerance (55°c, 20 min); however, after 5 min at 60°c in phosphate buffer a two log units decrease in cell viability was observed. different heat shock media were tested to improve the cell survival. best results were obtained in the mediumcontaining ino ... | 2017 | 28594955 |
| effect of lactobacillus rhamnosus gr-1 supernatant on cytokine and chemokine output from human amnion cells treated with lipoteichoic acid and lipopolysaccharide. | preterm birth occurs in 9% to 13% of all human pregnancies and accounts for 80% of all neonatal morbidities and mortalities. approximately 40% of all preterm births are idiopathic and about half are associated with infection and/or an activated inflammatory process. further to studies showing anti-inflammatory effects of supernatant from the probiotic lactobacillus rhamnosus gr-1 (gr-1), we tested its ability to modulate cytokine and chemokine production from amnion cells in response to stimulat ... | 2017 | 28587570 |
| probiotics for the prevention of nosocomial diarrhea in children. | this document provides recommendations developed by the espghan working group (wg) on probiotics and prebiotics on the role of probiotics in the prevention of nosocomial diarrhea in children based on a systematic review of previously completed systematic reviews and of subsequently published randomized controlled trials (rcts). the quality of evidence was assessed using the grading of recommendations assessment, development and evaluation (grade) guidelines. recommendations were given only if at ... | 2017 | 28574970 |
| lactobacillus rhamnosus atcc 7469 exopolysaccharides synergizes with low level ionizing radiation to modulate signaling molecular targets in colorectal carcinogenesis in rats. | combination therapy that targets cellular signaling pathway represents an alternative therapy for the treatment of colon cancer (crc). the present study was therefore aimed to investigate the probable interaction of lactobacillus rhamnosus atcc 7469 exopolysaccharides (eps) with low level ionizing γ radiation (γ-r) exposure against dimethylhydrazine (dmh)- induced colorectal carcinogenesis in rats. colon cancer was induced with 20mg dmh/kg bw. rats received daily by gastric gavage 100mg eps/kg b ... | 2017 | 28554134 |
| the role of microbiota and immunobiotics in granulopoiesis of immunocompromised hosts. | the number of granulocytes is maintained by a regulated balance between granulopoiesis in the bone marrow and clearance and destruction in peripheral tissues. granulopoiesis plays a fundamental role in the innate immune response. therefore, factors affecting the normal granulopoiesis lead to alterations in innate defenses and reduce the resistance against infections. in this study, we give a description on recent advances regarding the molecular and cellular events that regulate steady-state and ... | 2017 | 28533775 |
| a novel millet-based probiotic fermented food for the developing world. | probiotic yogurt, comprised of a fiti sachet containing lactobacillus rhamnosus gr-1 and streptococcus thermophilus c106, has been used in the developing world, notably africa, to alleviate malnutrition and disease. in sub-saharan african countries, fermentation of cereals such as millet, is culturally significant. the aim of this study was to investigate the fermentation capability of millet when one gram of the fiti sachet consortium was added. an increase of 1.8 and 1.4 log cfu/ml was observe ... | 2017 | 28531168 |
| enhancement of antioxidative and intestinal anti-inflammatory activities of glycated milk casein after fermentation with lactobacillus rhamnosus 4b15. | in this study, we investigated the glycoproteomics of glycated milk casein (gmc) and gmc fermented by lactobacillus rhamnosus 4b15 (fgmc) and determined their biological implications. there was a significant increase in the antioxidative and anti-inflammatory activities of gmc with galactose, which were higher than those of gmc with glucose (gmc-glc). furthermore, the fermentation of gmc by l. rhamnosus 4b15 synergistically enhanced the above activities compared to those of unfermented gmc. espe ... | 2017 | 28510450 |
| lactobacillus rhamnosus crl1505 nasal administration improves recovery of t-cell mediated immunity against pneumococcal infection in malnourished mice. | immunobiotic lactic acid bacteria have become an interesting alternative for the prevention of respiratory infections. previously, we demonstrated that the nasal administration of lactobacillus rhamnosus crl1505, during repletion of malnourished mice, resulted in diminished susceptibility to the challenge with the respiratory pathogen streptococcus pneumoniae. considering the known alterations induced by malnutrition on t lymphocytes and the importance of this cell population on the protection a ... | 2017 | 28504568 |
| genotypic and phenotypic diversity of lactobacillus rhamnosus clinical isolates, their comparison with strain gg and their recognition by complement system. | lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin. however, in some cases, lactobacillus spp. have been implicated in bacteremia. the aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of l. rhamnosus and to compare them to the well-studied l. rhamnosus probiotic strain gg. blood cultures from bacteremi ... | 2017 | 28493885 |
| fermentation of protopanaxadiol type ginsenosides (pd) with probiotic bifidobacterium lactis and lactobacillus rhamnosus. | ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities. in this study, major protopanaxadiol type ginsenosides (pd) rb1, rc, and rb2 were isolated from panax ginseng and were transformed using two probiotic strains namely bifidobacteri ... | 2017 | 28478490 |
| endocarditis due to lactobacillus rhamnosus in a patient with bicuspid aortic valve: potential role for the consumption of probiotics? | | 2017 | 28476455 |
| alginate based nanocomposite for microencapsulation of probiotic: effect of cellulose nanocrystal (cnc) and lecithin. | probiotic (lactobacillus rhamnosus atcc 9595) was encapsulated in alginate-cnc-lecithin microbeads to produce nutraceutical microcapsules. addition of cnc and lecithin in alginate microbeads (acl-1) improved the viability of l. rhamnosus during gastric passage and storage. the compression strength of the freeze-dried acl-1 microbeads improved 40% compared to alginate microbeads alone. swelling studies revealed that addition of cnc and lecithin in alginate microbeads decreased (around 47%) the ga ... | 2017 | 28457464 |
| a survey of probiotic use practices among patients at a tertiary medical centre. | probiotic use has skyrocketed in recent years. little is known, however, about patient knowledge and practices regarding probiotic use, especially in the context of antibiotic use. an invitation to complete a short, anonymous, electronic survey was sent by email to 965 patients at a tertiary medical centre in california who had agreed to be contacted for participation in research studies. questions were asked about both probiotic and antibiotic use in the prior three months. of 333 survey respon ... | 2017 | 28403649 |
| the mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria. | reconstituted skim milk (rsm) is a reputed protective carrier for improving the survival ratio of lactic acid bacteria (lab) after spray drying; however the underlying mechanisms of the prominent protection remains unclear. in this study, the inactivation histories of two lab strains during droplet drying with four carriers were experimentally determined, and the effects of droplet drying parameters on lab inactivation were investigated. for the first time, the possible contribution of each rsm ... | 2015 | 28455028 |