| a probiotic strain of l. acidophilus reduces dmh-induced large intestinal tumors in male sprague-dawley rats. | probiotic bacteria strains were examined for their influence on 1,2-dimethylhydrazine (dmh)-induced intestinal tumors in 100 male sprague-dawley rats. lactobacillus acidophilus (delvo pro la-1), lactobacillus rhamnosus (gg), bifidobacterium animalis (cscc1941), and streptococcus thermophilus (dd145) strains were examined for their influence when added as freeze-dried bacteria to an experimental diet based on a high-fat semipurified (ain-93) rodent diet. four bacterial treatments were compared: l ... | 1999 | 10693169 |
| binding of aflatoxin b1 alters the adhesion properties of lactobacillus rhamnosus strain gg in a caco-2 model. | lactic acid bacteria have been previously reported to possess antimycotoxigenic activities both in vitro and in vivo. the objective of this study was to investigate the effect of aflatoxin b1 on adhesion capability of lactobacillus rhamnosus strain gg using a caco-2 adhesion model. removal of aflatoxin b1 by l. rhamnosus strain gg reduced the adhesion capability of this strain from 30% to 5%. it is therefore concluded that aflatoxins may influence the adhesion properties of probiotics able to se ... | 2000 | 10716575 |
| temporal temperature gradient gel electrophoresis (ttge) as a tool for identification of lactobacillus casei, lactobacillus paracasei, lactobacillus zeae and lactobacillus rhamnosus. | to develop a tool for rapid and inexpensive identification of the lactobacillus casei complex. | 2001 | 11298928 |
| identification of salivary lactobacillus rhamnosus species by dna profiling and a specific probe. | the lactobacillus genus has been shown to be associated with the dental carious process, but little is known about the species related to the decay, although lactobacillus rhamnosus is suspected to be the most implicated species. conventional identification methods based on biochemical criteria lead to ambiguous results, since the lactobacillus species found in saliva are phenotypically close. to clarify the role of this genus in the evolution of carious disease, this work aimed to find a rapid ... | 2001 | 11316369 |
| probiotic activities of lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties. | the interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. in this study, the probiotic activities of a human isolate of lactobacillus casei subsp. rhamnosus strain (lcr35) were investigated. using intestinal caco-2 cell line in an in vitro model, we demonstrated that this strain exhibited adhesive properties. the inhibitory effects of ... | 2001 | 11316370 |
| the production of mixed cultures containing strains of lactococcus lactis, leuconostoc cremoris and lactobacillus rhamnosus, on commercial starter media. | mixed starters containing lactococcus lactis, leuconostoc cremoris and lactobacillus rhamnosus strains were produced on commercial starter media (mb complete, thermolac, marlac), as well as on milk. with the exception of marlac, the starters were cultured under ph control. the effect of media and incubation temperature (22 or 32 degrees c) on population ratios, on specific acidifying activities (saa) of the cultures as well as on their ability to produce aroma compounds in milk was studied. the ... | 2000 | 11320415 |
| the effect of supplementation by different nitrogen sources on the production of lactic acid from date juice by lactobacillus casei subsp. rhamnosus. | production of lactic acid from date juice by fermentation has been studied using lactobacillus casei subsp. rhamnosus as the producer organism. the optimum substrate concentration, expressed in its glucose content, was 60 g l(-1). various nitrogen sources were compared with yeast extract in terms of their efficiency for lactic acid production. none of these nitrogen sources gave lactic acid concentrations as high as that obtained with yeast extract. as yeast extract supplementation was not econo ... | 2001 | 11333033 |
| differences in the gut bacterial flora of healthy and milk-hypersensitive adults, as measured by fluorescence in situ hybridization. | we enumerated the predominant gut genera from fecal samples of nine healthy and eight milk-hypersensitive adults both before and after 4 weeks lactobacillus rhamnosus gg (lgg) supplementation. the anaerobic intestinal microflora of milk-hypersensitive adults was found to resemble that of healthy adults. lgg-consumption resulted in a significant increase in the number of bifidobacteria in healthy but not in milk-hypersensitive subjects, as well as a general increase in bacterial numbers in all ot ... | 2001 | 11335141 |
| results of a double-blind, randomized study to evaluate the efficacy and safety of antibiophilus in patients with radiation-induced diarrhoea. | gastrointestinal toxicity is frequently observed during radiotherapy for malignancies in the abdomen and pelvis. this study was performed to determine the efficacy and tolerability of lactobacillus rhamnosus (antibiophilus) in comparison to placebo in a double-blind trial design. its aim was to determine any clinically relevant difference between antibiophilus and placebo in terms of efficacy in patients suffering from mild to moderate diarrhoea induced by radiation therapy. | 2001 | 11338068 |
| probiotic agents and infectious diseases: a modern perspective on a traditional therapy. | there is an increasing scientific and commercial interest in the use of beneficial microorganisms, or "probiotics," for the prevention and treatment of disease. the microorganisms most frequently used as probiotic agents are lactic-acid bacteria such as lactobacillus rhamnosus gg (lgg), which has been extensively studied in recent literature. multiple mechanisms of action have been postulated, including lactose digestion, production of antimicrobial agents, competition for space or nutrients, an ... | 2001 | 11340528 |
| systemic immunity-enhancing effects in healthy subjects following dietary consumption of the lactic acid bacterium lactobacillus rhamnosus hn001. | to determine the effects of the probiotic lactic acid bacterium, lactobacillus rhamnosus hn001, on natural cellular immunity when delivered orally in normal low-fat milk (lfm) or lactose-hydrolyzed low-fat milk (lfm-lh). | 2001 | 11349938 |
| suppression of t-cell activation by lactobacillus rhamnosus gg-degraded bovine casein. | earlier data indicate that lactobacillus rhomnosus gg atcc 53103 (l. gg), a commensal intestinal bacterial strain, promotes the degradation of proteins in the gut in vivo, and bovine casein hydrolysed with l. gg-derived proteases suppresses lymphocyte proliferation in vitro. the present study aimed to evaluate the effect of l. gg-degraded bovine casein on t-cell activation, i.e. il-2 mrna expression and protein kinase c (pkc) translocation. to this end, northern blot analyses for il-2 mrna expre ... | 2001 | 11360922 |
| characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish. | the present study aimed to investigate the potential probiotic properties of six lactic acid bacteria (lab) intended for human use, lactobacillus rhamnosus atcc 53103, lactobacillus casei shirota, lactobacillus bulgaricus, l. rhamnosus lc 705, bifidobacterium lactis bb12, and lactobacillus johnsonii la1, and one for animal use, enterococcus faecium tehobak, for use as a fish probiotic. the strains for human use were specifically chosen since they are known to be safe for human use, which is of m ... | 2001 | 11375147 |
| detection of lactobacillus, pediococcus, leuconostoc, and weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis. | denaturing gradient gel electrophoresis (dgge) of dna fragments generated by pcr with 16s ribosomal dna-targeted group-specific primers was used to detect lactic acid bacteria (lab) of the genera lactobacillus, pediococcus, leuconostoc, and weissella in human feces. analysis of fecal samples of four subjects revealed individual profiles of dna fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria s ... | 2001 | 11375166 |
| use of intravaginal microbicides to prevent acquisition of trichomonas vaginalis infection in lactobacillus-pretreated, estrogenized young mice. | d2a21, a novel peptide antibiotic has in vitro activity against a wide spectrum of sexually transmitted diseases (std). in this study we tested the hypothesis that intravaginal d2a21 would interfere with acquisition of trichomonas vaginalis infection in a modified mouse model. t. vaginalis infections of estrogenized young mice pretreated with lactobacillus vaginalis or lactobacillus rhamnosus were more frequent and persistent than those in mice pre-treated with lactobacillus gasseri or lactobaci ... | 2000 | 11421379 |
| effects of lactobacillus rhamnosus gg and lactobacillus reuteri r2lc on acetic acid-induced colitis in rats. | certain lactobacilli reduce the severity of experimental colitis. the aim of this study was to compare the effects of a human strain lactobacillus rhamnosus gg and a rat strain lactobacillus reuteri r2lc on acetic acid-induced colitis in rats. | 2001 | 11424322 |
| surface binding of aflatoxin b(1) by lactic acid bacteria. | specific lactic acid bacterial strains remove toxins from liquid media by physical binding. the stability of the aflatoxin b(1) complexes formed with 12 bacterial strains in both viable and nonviable (heat- or acid-treated) forms was assessed by repetitive aqueous extraction. by the fifth extraction, up to 71% of the total aflatoxin b(1) remained bound. nonviable bacteria retained the highest amount of aflatoxin b(1). lactobacillus rhamnosus strain gg (atcc 53103) and l. rhamnosus strain lc-705 ... | 2001 | 11425726 |
| probiotics: "living drugs". | the uses, mechanisms of action, and safety of probiotics are discussed. probiotics are live microorganisms or microbial mixtures administered to improve the patient's microbial balance, particularly the environment of the gastrointestinal tract and the vagina. the yeast saccharomyces boulardii and the bacterium lactobacillus rhamnosus, strain gg, have shown efficacy in clinical trials for the prevention of antimicrobial-associated diarrhea. other probiotics that have demonstrated at least some p ... | 2001 | 11449853 |
| good adhesion properties of probiotics: a potential risk for bacteremia? | the ability to adhere to human intestinal mucus was tested for lactic acid bacteria of clinical blood culture, human fecal and dairy origin. the blood culture isolates were found to adhere better than the dairy strains. of the lactobacillus rhamnosus strains (nine clinical, 10 fecal and three dairy), blood culture isolates adhered better than the fecal strains. although these results indicate a trend for blood culture isolates to bind to intestinal mucus in higher numbers than strains of dairy a ... | 2001 | 11476979 |
| lactobacillus casei subsp. rhamnosus sepsis in a patient with ulcerative colitis. | lactobacillus species are part of normal gastrointestinal flora and are an uncommon cause of bacteremia. lactobacillus casei subsp. rhamnosus was repeatedly isolated in the blood cultures of a 43-year-old woman with ulcerative colitis. lactobacillus spp. could be considered a potential opportunistic pathogen whenever there is disruption of the physiologic architecture of the intestinal mucosa, such as in abdominal surgery, endoscopic procedures, or enteric pathologies. clinicians and clinical mi ... | 2001 | 11500620 |
| viability and dose-response studies on the effects of the immunoenhancing lactic acid bacterium lactobacillus rhamnosus in mice. | previous studies have indicated that the lactic acid bacterium lactobacillus rhamnosus hn001 can enhance immune function in mice, following oral delivery. however, the influence of bacterial cell viability on immunoenhancement, and the optimum dose of hn001 required for this effect, have not been determined. in the present study, both live and heat-killed preparations of l. rhamnosus hn001 were shown to enhance the phagocytic activity of blood and peritoneal leucocytes in mice, at a dose of 109 ... | 2001 | 11502243 |
| streptococcus thermophilus in cheddar cheese--production and fate of galactose. | the behaviour of streptococcus thermophilus in combination with lactococcus lactis subsp. cremoris or subsp. lactis mesophilic starters in experimental cheddar cheese is reported. in a standard manufacturing procedure employing a 38 degrees c cook temperature, even very low levels (0.007%) of str. thermophilus combined with normal levels of the mesophilic starter (1.7%) resulted in increased rates of acid production, the formation of significant amounts of galactose (approximately 13 mmol/kg che ... | 2001 | 11504394 |
| dietary probiotic supplementation enhances natural killer cell activity in the elderly: an investigation of age-related immunological changes. | many elderly subjects are at increased risk of infectious and noninfectious diseases due to an age-related decline in lymphoid cell activity (immunosenescence). noninvasive means of enhancing cellular immunity are therefore desirable in the elderly. previous reports have suggested that dietary supplementation could represent an effective means of enhancing the activity of circulating natural killer (nk) cells in the elderly. in the present study, we have conducted a pre-post intervention trial t ... | 2001 | 11506196 |
| in vitro adherence properties of lactobacillus rhamnosus dr20 and bifidobacterium lactis dr10 strains and their antagonistic activity against an enterotoxigenic escherichia coli. | adhesion and colonisation properties of three probiotic strains namely, lactobacillus rhamnosus dr20, l. acidophilus hn017, and bifidobacterium lactis dr10, were determined in vitro using the differentiated human intestinal cell-lines including ht-29, caco-2, and ht29-mtx, and compared with properties of l. acidophilus la-1 and l. rhamnosus gg (two commercial probiotic strains). two independent methods were employed to quantitate the "adhesiveness" of each strain. in the first method, the bacter ... | 2001 | 11518430 |
| lactobacillus rhamnosus endocarditis complicating colonoscopy. | we report the first case of endocarditis caused by lactobacillus after an uneventful colonoscopy. the initial empiric treatment with the standard regimen of penicillin-aminoglycoside failed; subsequent treatment with a combination of antibiotics, selected according to the in vitro studies, was successful. | 2001 | 11545575 |
| induction of nitric oxide synthesis by probiotic lactobacillus rhamnosus gg in j774 macrophages and human t84 intestinal epithelial cells. | probiotic lactobacillus rhamnosus gg (lgg) has proved to be beneficial in the treatment of viral- and antibiotic-associated diarrhea but the mechanisms of action remain unknown. nitric oxide (no) is involved in the protective mechanisms in the gastrointestinal tract and may contribute to some of the beneficial effects of probiotics. the aim of the present study was to investigate if induction of no synthesis is involved in the cellular actions of lgg. | 2001 | 11580098 |
| an improved procedure for characterization of spatial and temporal evolution of immobilized cells in gel membranes. | an improved procedure that allows the simple and reproducible characterization of spatial and temporal distribution of immobilized biomass in gel membranes was developed. this procedure involves three main steps in the preparation of membrane samples, the use of a standard microtome to obtain membrane slices, and the measurement of cell concentration by spectrophotometry. the key improvement in this procedure is to prepare the membrane samples by clamping them between two glass plates and storin ... | 2001 | 11601616 |
| endocarditis by lactobacillus rhamnosus due to yogurt ingestion? | a young man who ate large quantities of probiotic yogurt developed endocarditis and septic arthritis caused by lactobacillus rhamnosus. the pathogenic isolate could not be distinguished from the yogurt microflora using methods routinely used in the clinical microbiology laboratory. only by using more appropriate methodology, including pcr, the pathogen could be distinguished from the yogurt isolate. | 2001 | 11669233 |
| phosphatidylinositol-specific phospholipase c activity in lactobacillus rhamnosus with capacity to translocate. | phosphatidylinositol-specific phospholipase c (pi-plc) activity was investigated in 25 different lactic acid bacteria (lab) strains belonging to the genera lactobacillus, weisella, and enterococcus. pi-plc activity was detected in 44% of the strains studied in culture medium without carbon source. from the pi-plc positive strains, lactobacillus rhamnosus atcc 7469 was selected for translocation studies. healthy mice were orally administered with a daily dose of 2.0 x 10(9) of viable l. rhamnosus ... | 2001 | 11682174 |
| aflatoxin b1 binding by dairy strains of lactic acid bacteria and bifidobacteria. | various food commodities including dairy products may be contaminated with aflatoxins, which, even in small quantities, have detrimental effects on human and animal health. several microorganisms have been reported to bind or degrade aflatoxins in foods and feeds. this study assessed the binding of aflatoxin b1 (afb1) from contaminated solution by 20 strains of lactic acid bacteria and bifidobacteria. the selected strains are used in the food industry and comprised 12 lactobacillus, five bifidob ... | 2001 | 11699445 |
| catheter-related bacteremia due to lactobacillus rhamnosus in a single-lung transplant recipient. | there is still a major debate about the pathogenicity of lactobacillus spp. and some reports emphasize that these microorganisms are never isolated from endovascular devices. in this report we present a case of catheter-related bacteremia due to l. rhamnosus in a patient who underwent a single-lung transplant. | 2001 | 11728051 |
| probiotic lactobacillus dose required to restore and maintain a normal vaginal flora. | forty-two healthy women were randomized to receive one of three encapsulated lactobacillus rhamnosus gr-1 plus lactobacillus fermentum rc-14 probiotic dosage regimens or l. rhamnosus gg by mouth each day for 28 days. however, the vaginal flora, assessed by nugent scoring, was only normal in 40% of the cases, and 14 patients had asymptomatic bacterial vaginosis. treatment with l. rhamnosus gr-1/l. fermentum rc-14 once and twice daily correlated with a healthy vaginal flora in up to 90% of patient ... | 2001 | 11750220 |
| new method for exopolysaccharide determination in culture broth using stirred ultrafiltration cells. | a new method to remove simple carbohydrates from culture broth prior to the quantification of exopolysaccharides (eps) was developed and validated for the eps-producing strain, lactobacillus rhamnosus rw-9595m. this method uses ultrafiltration (uf) in stirred cells followed by polysaccharide detection in the retentate by the phenol-sulfuric acid method. the uf method was compared with a conventional method based on ethanol extraction, dialysis, protein removal by trichloroacetic acid (tca) and f ... | 2001 | 11759693 |
| gradient diffusion antibiotic susceptibility testing of potentially probiotic lactobacilli. | minimum inhibitory contentrations (mics) of selected inhibitors of cell wall synthesis (benzylpenicillin, ampicillin, and vancomycin), protein synthesis (gentamicin, streptomycin, tetracycline, chloramphenicol, and erythromycin), and nucleic acid synthesis (co-trimoxazole, rifampicin, and metronidazole) were determined by gradient diffusion (e test; ab biodisk, solna, sweden) on deman, rogosa, sharpe (mrs) agar for lactobacillus strain gg and 11 closely related, rapidly growing, facultatively an ... | 2001 | 11770631 |
| cloning and expression of an oligopeptidase, pepo, with novel specificity from lactobacillus rhamnosus hn001 (dr20). | oligopeptidases of starter and nonstarter lactic acid bacteria contribute to the proteolytic events important in maturation and flavor development processes in cheese. this paper describes the molecular cloning, expression, and specificity of the oligopeptidase pepo from the probiotic nonstarter strain lactobacillus rhamnosus hn001 (dr20). the pepo gene encodes a protein of 70.9 kda, whose primary sequence includes the hexxh motif present in certain classes of metallo-oligopeptidases. the pepo g ... | 2002 | 11772634 |
| persistence of lactobacillus fermentum rc-14 and lactobacillus rhamnosus gr-1 but not l. rhamnosus gg in the human vagina as demonstrated by randomly amplified polymorphic dna. | lactobacillus rhamnosus gr-1 and l. fermentum rc-14 are well-characterized probiotic strains with efficacy in the prevention and treatment of urogenital infections in women. the aim of the present study was to apply a molecular biology-based methodology for the detection of these strains and l. rhamnosus gg (a commercially available intestinal probiotic) in the human vagina in order to assess probiotic persistence at this site. ten healthy women inserted vaginally a capsule containing either a c ... | 2002 | 11777835 |
| continuous lactic acid fermentation using a plastic composite support biofilm reactor. | an immobilized-cell biofilm reactor was used for the continuous production of lactic acid by lactobacillus casei subsp. rhamnosus (atcc 11443). at iowa state university, a unique plastic composite support (pcs) that stimulates biofilm formation has been developed. the optimized pcs blend for lactobacillus contains 50% (wt/wt) agricultural products [35% (wt/wt) ground soy hulls, 5% (wt/wt) soy flour, 5% (wt/wt) yeast extract, 5% (wt/wt) dried bovine albumin, and mineral salts] and 50% (wt/wt) pol ... | 2001 | 11778870 |
| efficient protoplast regeneration for some homofermentative lactobacilli and pediococci. | conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. replacing or increasing colloidal substances in a medium containing raffinose and mgcl(2) as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10-99%. a medium containing ge ... | 2001 | 11797042 |
| the predominant microflora of nursing caries lesions. | the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ... | 2001 | 11799279 |
| effect of long-term consumption of a probiotic bacterium, lactobacillus rhamnosus gg, in milk on dental caries and caries risk in children. | lactobacillus rhamnosus gg, atcc (lgg), has shown antagonism to many bacteria including mutans streptococci. this randomized, double-blind, placebo-controlled intervention study was designed to examine whether milk containing lgg has an effect on caries and the risk of caries in children when compared with normal milk. 594 children, 1-6 years old, from 18 municipal day-care centres were included. the children received the milk with meals from coded containers 5 days a week in the day-care centre ... | 2001 | 11799281 |
| chemopreventive effect of lactobacillus rhamnosus on growth of a subcutaneously implanted bladder cancer cell line in the mouse. | lactic acid bacteria are known to have beneficial effects on the host, such as preventing carcinogenesis. the present study was designed to evaluate the chemopreventive effects of lactobacillus rhamnosus strain gg (lgg) in suppressing bladder cancer formation in a murine subcutaneous model of bladder cancer involving the inoculation of mb49 cells in c57b / l6 mice. after tumor implantation, one group of mice (n = 8) was fed lgg immediately. the remaining mice that had tumors between 0.03 - 0.1 c ... | 2002 | 11802806 |
| selective in vitro binding of dietary mutagens, individually or in combination, by lactic acid bacteria. | specific strains of lactic acid bacteria possessing antimutagenic properties are suggested to remove mutagenic contaminants of foods through binding and an investigation of their substrate specificity is required. the ability of lactobacillus rhamnosus strains gg and lc-705 in viable and non-viable (heat- and acid-treated) forms to remove both dietary mutagens and other aromatic dietary substrates from solution was studied using hplc. overall, removal increased in the order: caffeine = vitamin b ... | 2002 | 11820495 |
| protection against translocating salmonella typhimurium infection in mice by feeding the immuno-enhancing probiotic lactobacillus rhamnosus strain hn001. | the probiotic lactic acid bacterium lactobacillus rhamnosus (strain hn001) is known to stimulate enhanced innate and acquired immune responses in mice. following oral delivery. here, the ability of hn001 to confer immune enhancement and protection against an oral challenge of salmonella typhimurium was investigated. hn001-fed and non-probiotic-fed control balb/c mice were challenged with either a single dose of s. typhimurium (atcc strain 1772), or with five repeated daily doses of the pathogen; ... | 2001 | 11827205 |
| stability and activity of specific antibodies against streptococcus mutans and streptococcus sobrinus in bovine milk fermented with lactobacillus rhamnosus strain gg or treated at ultra-high temperature. | passive local immunization against dental caries is a promising approach to its prevention, as clinical evidence of active oral or nasal immunization is still limited and controversial. by means of systemic immunization of pregnant cows with a multivalent vaccine, high titres of igg antibodies against human cariogenic bacteria, streptococcus mutans and streptococcus sobrinus, were produced in bovine colostrum. the purified immune product (ip) of this preparation has a number of anticariogenic pr ... | 2002 | 11860550 |
| quantification of exopolysaccharide, lactic acid, and lactose concentrations in culture broth by near-infrared spectroscopy. | near-infrared spectroscopy (nirs) was used for the simultaneous prediction of exopolysaccharide (eps; 0-3 g/l) and lactic acid (0-59 g/l) productions as well as lactose (0-68 g/l) concentration in supernatant samples from ph-controlled batch cultures of lactobacillus rhamnosus rw-9595m in supplemented whey permeate medium. to develop calibration equations, the correlation between the second derivative of 164 nirs transmittance spectra and concentration data obtained with reference methods was ca ... | 2002 | 11902911 |
| structure determination of the exopolysaccharide produced by lactobacillus rhamnosus strains rw-9595m and r. | exopolysaccharides (epss) were isolated and purified from lactobacillus rhamnosus strains rw-9595m, which has been shown to possess cytokine-stimulating activity, and r grown under various fermentation conditions (carbon source, incubation temperature and duration). identical (1)h nmr spectra were obtained in all cases. molecular masses were determined by gel permeation chromatography. the primary structure was elucidated using chemical and spectroscopic techniques. organic acid, monosaccharide ... | 2002 | 11903041 |
| no effect of oral treatment with an intestinal bacterial strain, lactobacillus rhamnosus (atcc 53103), on birch-pollen allergy: a placebo-controlled double-blind study. | oral probiotic bacteriotherapy with lactobacillus rhamnosus has given promising results in small children with food allergy. we studied the effects of similar therapy in teenagers and young adults, who were allergic to birch pollen and apple food and had intermittent symptoms of atopic allergy and/or mild asthma. | 2002 | 11906339 |
| lactobacillus rhamnosus septicaemia in a patient with a graft in the inferior vena cava. | a case of septicaemia that repeatedly showed growth of lactobacillus rhamnosus in blood cultures is reported. the patient improved after removal of a goretex patch in the inferior vena cava that was the focus of the infection. lactobacilli can, in certain settings, be the cause of clinically important infections. | 2002 | 11928847 |
| immune enhancement conferred by oral delivery of lactobacillus rhamnosus hn001 in different milk-based substrates. | the probiotic lactobacillus rhamnosus hn001 is known to enhance immunity in animal models, following oral delivery. in this study, we investigated the immunoenhancing effects of hn001 delivered to mice in different milk-based substrates, including: whole (full-fat) milk supplemented with hn001; fermented milk supplemented with hn001; or whole milk which had been part-fermented by hn001. direct oral feeding of mice with hn001 in whole milk was shown to enhance the phagocytic activity of blood and ... | 2001 | 11928957 |
| combined effects of temperature and medium composition on exopolysaccharide production by lactobacillus rhamnosus rw-9595m in a whey permeate based medium. | the effects of temperature (22-42 degrees c), whey permeate concentration (wp, 1.6-8.4%), and supplementation level with yeast nitrogen base (ynb, 0-2.0%) on exopolysaccharide (eps) production was studied during 20 ph-controlled (ph = 6.0) batch cultures with lactobacillus rhamnosus rw-9595m, using a central composite design (ccd). the eps production was measured using both the conventional method based on ethanol precipitation of eps and a new ultrafiltration (uf) method. eps production was not ... | 2002 | 11934282 |
| increased enterocyte production in gnotobiotic rats mono-associated with lactobacillus rhamnosus gg. | there is increasing scientific and commercial interest in using beneficial microorganisms (i.e., probiotics) to enhance intestinal health. of the numerous microbial strains examined, lactobacillus rhamnosus gg has been most extensively studied. daily intake of l. rhamnosus gg shortens the course of rotavirus infection by mechanisms that have not been fully elucidated. comparative studies with germfree and conventional rats have shown that the microbial status of an animal influences the intestin ... | 2002 | 12039764 |
| safety and efficacy of probiotic lactobacilli in promoting growth in post-weaning swiss mice. | lactobacillus rhamnosus ma27/6b and l. acidophilus ma27/6r are strains used in feed as probiotics. their safety profiles and growth-stimulating properties were investigated via in vivo studies on young swiss mice. after repeated administrations of different probiotic preparations in drinking water, safety parameters determined from liver, spleen and total weight remained unchanged. the growth-stimulating properties of viable or dead lactobacilli were studied after supplementation in drinking wat ... | 2002 | 12076035 |
| binding rather than metabolism may explain the interaction of two food-grade lactobacillus strains with zearalenone and its derivative (')alpha-earalenol. | the interaction between two fusarium mycotoxins, zearalenone (zen) and its derivative (')alpha-zearalenol ((')alpha-zol), with two food-grade strains of lactobacillus was investigated. the mycotoxins (2 microg ml(-1)) were incubated with either lactobacillus rhamnosus strain gg or l. rhamnosus strain lc705. a considerable proportion (38 to 46%) of both toxins was recovered from the bacterial pellet, and no degradation products of zen and (')alpha-zol were detected in the high-performance liquid ... | 2002 | 12089040 |
| structural studies of the exopolysaccharide produced by lactobacillus rhamnosus strain gg (atcc 53103). | the structure of the extracellular polysaccharide (eps) from lactobacillus rhamnosus strain gg has been investigated. in combination with component analysis, nmr spectroscopy shows that the polysaccharide is composed of hexasaccharide repeating units. sequential information was obtained by two-dimensional (1)h,(1)h-noesy, and (1)h,(13)c-hmbc nmr techniques. the structure of the repeating unit of the eps from lactobacillus rhamnosus strain gg was determined as: [carbohydrate structure: see text] | 2002 | 12099838 |
| removal of common fusarium toxins in vitro by strains of lactobacillus and propionibacterium. | this study was conducted to examine the ability of selected strains of lactobacillus and propionibacterium to remove common fusarium toxins, trichothecenes, from liquid media. the trichothecenes studied were deoxynivalenol (don), 3-acetyldeoxynivalenol (3-acdon), nivalenol (niv), fusarenon (fx), diacetoxyscirpenol (das), t-2 toxin (t-2) and ht-2 toxin (ht-2). the lactobacillus rhamnosus strain gg (lgg), lactobacillus rhamnosus strain lc-705 (lc-705) and propionibacterium freudenreichii ssp. sher ... | 2002 | 12113664 |
| antifungal activity of sodium acetate and lactobacillus rhamnosus. | the inhibition of molds by sodium acetate in deman rogosa sharpe (mrs) medium, along with the antifungal activity of lactobacillus rhamnosus vt1, was studied by the slope agar plate method. mrs agar prepared with and without sodium acetate was used as the agar substrate. a total of 42 strains of aspergillus, penicillium, fusarium, alternaria, cladosporium, and rhizopus were used to compare sensitivities to the inhibitory activity of sodium acetate and l. rhamnosus vt1. it was found that sodium a ... | 2002 | 12117257 |
| dietary intake of lactobacillus rhamnosus hnoo1 enhances production of both th1 and th2 cytokines in antigen-primed mice. | probiotic lactobacilli have been proposed as a potential oral bacteriotherapeutic means of modulating immune phenotype expression in vivo, via their ability to promote cytokine production. this study investigated the ability of a known interferon (ifn)gamma-promoting probiotic (lactobacillus rhamnosus hnooi) to modulate cytokine production in mice expressing an on-going th2-type immune response. balb/c mice were primed to ovalbumin in alum adjuvant to invoke antigen-specific th2 cytokine-secreti ... | 2002 | 12137199 |
| effect of probiotic lactobacillus strains in young children hospitalized with acute diarrhea. | oral bacteriotherapy promotes recovery from acute childhood diarrhea, but few strains have been shown to have therapeutic potentials. we examined the effect of two newly identified probiotic lactobacillus strains in acute childhood diarrhea. | 2002 | 12150178 |
| effect of probiotic lactobacillus strains on acute diarrhea in a cohort of nonhospitalized children attending day-care centers. | certain strains of lactobacilli have been shown to promote recovery from rotavirus enteritis in hospitalized children. few studies have examined the effect of probiotics in nonhospitalized children with mild diarrhea. | 2002 | 12150179 |
| yoghurt biotherapy: contraindicated in immunosuppressed patients? | a fatal case of lactobacillus rhamnosus septicaemia after prolonged oral vancomycin for recalcitrant clostridium difficile infection is reported. the patient was immunosuppressed with cyclophosphamide and steroids for sjögren's syndrome. the administration of lactobacillus spp as "biotherapy" may be hazardous in such circumstances. | 2002 | 12151695 |
| effect of probiotics on constipation, fecal azoreductase activity and fecal mucin content in the elderly. | constipation is a common problem in elderly subjects, probiotics have been suggested to improve intestinal motility and reduce fecal enzyme activity. | 2002 | 12169860 |
| immune protection mediated by the probiotic lactobacillus rhamnosus hn001 (dr20) against escherichia coli o157:h7 infection in mice. | this study investigated the protective effects of feeding the immunoenhancing probiotic lactobacillus rhamnosus hn001 against escherichia coli o157:h7 infection in murine (balb/c and c57bl/6 mice) challenge infection models. mice were fed milk-based diets supplemented with l. rhamnosus hn001 (3 x 10(8) cfu g(-1)) for 7 days prior to and following oral challenge with e. coli o157:h7. morbidity and feed intake were measured for 1 week following challenge; pathogen translocation to spleen, liver an ... | 2002 | 12208607 |
| competition for adhesion between probiotics and human gastrointestinal pathogens in the presence of carbohydrate. | the adhesion of lactobacillus rhamnosus gg to human enterocyte-like caco-2 cells was not inhibited by eight carbohydrates tested, namely n-acetyl-glucosamine, galactose, glucose, fructose, fucose, mannose, methyl-alpha-d-mannopyranoside and sucrose. the degree of hydrophobicity predicted the adhesion of l. rhamnosus gg to caco-2 cells. l. rhamnosus gg, however, was able to compete with escherichia coli and salmonella spp. of low hydrophobicity and high adhesin-receptor interaction for adhesion t ... | 2002 | 12215184 |
| the evaluation of mixtures of yeast and potato extracts in growth media for biomass production of lactic cultures. | the effectiveness of yeast extracts (ye) and potato extracts (pe) to promote growth of seven lactic cultures was evaluated by automated spectrophotometry (as). two aspects of the growth curve were analysed: (1) maximum biomass obtained (using odmax) and (2) highest specific growth rate mu(max)) eleven lots from the same pe-manufacturing process were examined for lot-to-lot variability. the odmax values of three of the seven strains were significantly affected by lot source, but mu(max) was not s ... | 2002 | 12224561 |
| signalling mechanisms involved in the induction of inducible nitric oxide synthase by lactobacillus rhamnosus gg, endotoxin, and lipoteichoic acid. | probiotic lactobacillus rhamnosus gg (lactobacillus gg) has been found beneficial in the treatment of viral and antibiotic-associated diarrhea. recently, it has also been shown to induce nitric oxide (no) production, and have some other immunostimulatory effects. the aim of the present study was to investigate the mechanisms involved in the induction of inducible nitric oxide synthase (inos) and no production by lactobacillus gg. | 2002 | 12238563 |
| probiotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells. | probiotic bacteria are microorganisms that benefit the host by preventing or ameliorating disease. however, little information is known regarding the scientific rationale for using probiotics as alternative medicine. the purpose of this paper is to investigate the mechanisms of probiotic beneficial effects on intestinal cell homeostasis. we now report that one such probiotic, lactobacillus rhamnosus gg (lgg), prevents cytokine-induced apoptosis in two different intestinal epithelial cell models. ... | 2002 | 12393915 |
| preliminary evaluation of lactobacillus rhamnosus strain gg, a potential probiotic in dogs. | lactobacillus rhamnosus strain gg (lgg) has been studied extensively as a probiotic in humans. however, the ability of an organism to survive passage through the intestinal tract and exert beneficial effects cannot be directly extrapolated between species. this study evaluated the ability of lgg to survive gastrointestinal transit in dogs and assessed whether oral administration of lgg is safe, in order to determine whether studies evaluating the efficacy of lgg in the treatment of canine diseas ... | 2002 | 12395758 |
| probiotics and crohn's disease. | antibiotics are often employed in the treatment of crohn's disease, with interesting results. however indiscriminate suppression of intestinal bacteria may be harmful and long-term use of antibiotics is burdened by side-effects and by the risk of developing bacterial resistance. manipulation of enteric flora with probiotic compounds would be a possible and appealing alternative. first aim of our study has been to investigate the efficacy of this probiotic in reducing the endoscopic recurrence ra ... | 2002 | 12408444 |
| probiotics in antibiotic-associated diarrhoea. | antibiotic-associated diarrhoea is a common event. in some cases, it could represent a life-threatening event. clostridium difficile colitis is a further distinct complication of antibiotic administration. treatment options for antibiotic-associated diarrhoea and clostridium difficile colitis include supplementation with several types of probiotics, as overviewed in this paper. three randomised, double-blind, controlled clinical trials show a therapeutic effect of saccharomyces boulardii in anti ... | 2002 | 12408447 |
| lactobacillus bacteremia during a rapid increase in probiotic use of lactobacillus rhamnosus gg in finland. | lactobacilli supposedly have low pathogenicity; they are seldom detected in blood culture. lactobacillus rhamnosus gg, which originates indigenously in the human intestine, became available for use as a probiotic in 1990 in finland. we evaluated the possible effects of the increased probiotic use of l. rhamnosus gg on the occurrence of bacteremia due to lactobacilli. lactobacilli were isolated in 0.02% of all blood cultures and 0.2% of all blood cultures with positive results in helsinki univers ... | 2002 | 12410474 |
| molecular diagnosis of endocarditis due to lactobacillus casei subsp. rhamnosus. | we report a case of endocarditis due to lactobacillus casei subsp. rhamnosus. the bacterium isolated from blood cultures and from valve tissue specimens was identified using both phenotypical analysis and dna sequence analysis, which revealed that the rod profiles of the pathogens recovered from blood cultures and valve tissue specimens were the same. | 2002 | 12410496 |
| antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics lactobacillus rhamnosus and bifidobacterium lactis on azoxymethane-induced colon carcinogenesis in rats. | prebiotics such as fructans, and probiotics such as lactobacilli or bifidobacteria, or a combination of prebiotics and probiotics (synbiotics) are thought to be protective against colon cancer. therefore, we studied whether the prebiotic inulin enriched with oligofructose (raftilose-synergy1, briefly, synergy1, 10% of the diet), probiotics [bifidobacterium lactis (bb12) and lactobacillus rhamnosus (lgg), each at 5x10(8) c.f.u./g diet] or synbiotics (a combination of the two) protect rats against ... | 2002 | 12419846 |
| short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. | cheese is known to contain compounds that reduce the risk of dental caries. the long-term consumption of milk containing lactobacillus rhamnosus gg, atcc 53103 (lgg), has been shown to reduce caries risk in children. the aim of the present study was to examine whether short-term consumption of cheese containing lgg and lactobacillus rhamnosus lc 705 would diminish caries-associated salivary microbial counts in young adults. altogether, 74 18-35 year-old subjects completed this double-blinded, ra ... | 2002 | 12446187 |
| interaction between probiotic lactic acid bacteria and canine enteric pathogens: a risk factor for intestinal enterococcus faecium colonization? | selected probiotic lactic acid bacteria (lab) have been shown to elicit positive health effects particularly in humans. competitive exclusion of pathogens is one of the most important beneficial health claims of probiotic bacteria. the effect of probiotic lab on competitive exclusion of pathogens has been demonstrated in humans, chicken and pigs. in this study we evaluated the ability of certain lab strains (lactobacillus rhamnosus gg, bifidobacterium lactis bb12, lactobacillus pentosus uk1a, l. ... | 2003 | 12488075 |
| heat and osmotic stress responses of probiotic lactobacillus rhamnosus hn001 (dr20) in relation to viability after drying. | the viability of lactic acid bacteria in frozen, freeze-dried, and air-dried forms is of significant commercial interest to both the dairy and food industries. in this study we observed that when prestressed with either heat (50 degrees c) or salt (0.6 m nacl), lactobacillus rhamnosus hn001 (also known as dr20) showed significant (p < 0.05) improvement in viability compared with the nonstressed control culture after storage at 30 degrees c in the dried form. to investigate the mechanisms underly ... | 2003 | 12571012 |
| identification and characterization of lactobacillus helveticus pepo2, an endopeptidase with post-proline specificity. | a post-proline endopeptidase (pepo2) was detected in cell extracts from a genomic library of lactobacillus helveticus cnrz32 by using the synthetic substrate n-acetyl-beta-casein-(f203-209)-rho-nitroanilide in a coupled reaction with aminopeptidase n. isolates with activity for this substrate contained plasmids with visually indistinguishable restriction profiles. nucleotide sequence analysis revealed a 1,947-bp open reading frame, designated pepo2, encoding a putative 71.4-kda protein. analysis ... | 2003 | 12571057 |
| lactobacillus gg prevents recurrence of colitis in hla-b27 transgenic rats after antibiotic treatment. | bacteroides vulgatus induces colitis in gnotobiotic hla-b27 transgenic (tg) rats while broad spectrum antibiotics prevent and treat colitis in specific pathogen free (spf) tg rats although disease recurs after treatment ends. lactobacilli treat human pouchitis and experimental colitis. we investigated if lactobacillus rhamnosus gg (l gg) can prevent colitis in tg rats monoassociated with b vulgatus and if l gg or lactobacillus plantarum 299v (lp 299v) can treat established colitis in spf tg rats ... | 2003 | 12584218 |
| effect of probiotic lactobacillus strains in children with atopic dermatitis. | recent studies suggest that oral bacteriotherapy with probiotics might be useful in the management of atopic dermatitis (ad). | 2003 | 12589361 |
| no effect of oral treatment with an intestinal bacterial strain, lactobacillus rhamnosus (atcc 53103), on birch-pollen allergy: a placebo-controlled double-blind study. | | 2002 | 12613481 |
| effect of lactobacillus rhamnosus gg on ileal pouch inflammation and microbial flora. | preliminary trials of probiotics in preventing recurrent chronic pouchitis have been encouraging. | 2003 | 12622759 |
| persistence of colonization of intestinal mucosa by a probiotic strain, lactobacillus casei subsp. rhamnosus lcr35, after oral consumption. | the colonization by the probiotic lactobacillus casei subsp. rhamnosus lcr35 of the gastrointestinal tracts of mice and humans was studied. the mice were orally given 10(9) cfu of lcr35 either once or three times at 24-h intervals. a 16s ribosomal nucleic probe used in hybridization assays detected lcr35 in the feces of mice for up to 3 days after the feeding, at a level of 10(8) to 10(9) cfu/g of feces. in the human assay, 12 healthy volunteers were enrolled in a randomized trial and ingested l ... | 2003 | 12624065 |
| oral use of lactobacillus rhamnosus gr-1 and l. fermentum rc-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. | urogenital infections afflict an estimated one billion people each year. the size of this problem and the increased prevalence of multi-drug resistant pathogens make it imperative that alternative remedies be found. a randomized, placebo-controlled trial of 64 healthy women given daily oral capsules of lactobacillus rhamnosus gr-1 and lactobacillus fermentum rc-14 for 60 days showed no adverse effects. microscopy analysis showed restoration from asymptomatic bacterial vaginosis microflora to a n ... | 2003 | 12628548 |
| growth and molecular characterization of dental plaque microcosms. | (i) to compare the effects of feeding protocols upon the composition and stability of dental plaque microcosms formed in constant-depth film fermenters (cdff). (ii) to evaluate the utility of denaturing gradient gel electrophoresis (dgge) and culture methodologies for the investigation of such models. | 2003 | 12631201 |
| kinetics of adsorption and desorption of aflatoxin b1 by viable and nonviable bacteria. | the reactions involved in the binding (adsorption) and release (desorption) of aflatoxin b1 (afb1) to and from the surface of bacteria were investigated. viable and heat-killed lactobacillus rhamnosus gg, l. rhamnosus lc-705, and propionibacterium freudenreichii subsp. shermanii js were incubated in phosphate-buffered saline containing variable concentrations (0.0017 to 13.3 microg/ml) of afb1. the relationship between the bacterial surface hydrophobicity and the afb1 adsorption affinity was als ... | 2003 | 12636295 |
| interactions of lactic acid bacteria with human intestinal epithelial cells: effects on cytokine production. | as a participant in the mucosal immune response, the intestinal epithelial cell must respond to a variety of stimuli, including lactic acid bacteria (lab) consumed in the diet. the objective of this study was to compare the abilities of several strains of lab to modulate cytokine secretion by human intestinal epithelial cell (iec) line ht-29. certain strains of lactobacillus rhamnosus, lactobacillus delbrueckii, and lactobacillus acidophilus suppressed the production of the chemokine rantes by s ... | 2003 | 12636302 |
| structural analysis of the lactobacillus rhamnosus strain kl37c exopolysaccharide. | the exopolysaccharide from the lactic acid bacterium lactobacillus rhamnosus strain kl37c isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. the polysaccharide material was purified by gel permeation chromatography on an tsk hw-50 column and characterised using chemical and enzymatic methods. on the basis of sugar and methylation analysis and 1h, 13c, 1d and 2d nmr ... | 2003 | 12644373 |
| lactobacillus rhamnosus gg decreases tnf-alpha production in lipopolysaccharide-activated murine macrophages by a contact-independent mechanism. | animal studies and human clinical trials have shown that lactobacillus can prevent or ameliorate inflammation in chronic colitis. however, molecular mechanisms for this effect have not been clearly elucidated. we hypothesize that lactobacilli are capable of downregulating pro-inflammatory cytokine responses induced by the enteric microbiota. we investigated whether lactobacilli diminish production of tumour necrosis factor alpha (tnf-alpha) by the murine macrophage line, raw 264.7 gamma (no-), a ... | 2003 | 12675685 |
| preliminary investigation of the probiotic potential of lactobacillus rhamnosus strain gg in horses: fecal recovery following oral administration and safety. | this study was designed to evaluate whether lactobacillus rhamnosus strain gg (lgg), an extensively studied probiotic organism in humans, can colonize the intestines of adult horses and foals. lactobacillus rhamnosus strain gg was administered to adult horses at doses of 1 x 10(9) cfu/50kg bodyweight (bw)/day (group 1, 7 horses), 1 x 10(10) colony forming units/ 50kg bw/day (group 2, 7 horses) and 5 x 10(10) colony forming units/50kg bw/day (group 3, 7 horses) for 5 d. foals received 2 x 10(10) ... | 2003 | 12715981 |
| three new insertion sequence elements isldl2, isldl3, and isldl4 in lactobacillus delbrueckii: isolation, molecular characterization, and potential use for strain identification. | a group of new insertion sequence (is) elements, isldl2, isldl3, and isldl4, from lactobacillus delbrueckii subsp. lactis atcc 15808 was isolated, characterized, and used for strain identification together with isldl1, recently characterized as an l. delbrueckii is element belonging to the isl3 family. isldl2 was 1367 bp in size and had a 24 bp ir and an 8 bp dr. the single orf of isldl2 encoded a protein of 392 aa similar to transposases of the is256 family. isldl3 had a single orf encoding a p ... | 2003 | 12749837 |
| probiotics and the urologist. | emerging from the stigma of once being referred to as "snake oil", excellent scientific and clinical evidence now exists to indicate that probiotics do indeed have a role to play in medicine. the proper definition of probiotics is important "live microorganisms which when administered in adequate amounts confer a health benefit on the host", for several reasons. it rules out so-called probiotics that have no clinically proven, peer-reviewed data, and it states the need to have viable bacteria pr ... | 2003 | 12773227 |
| specific identification of certain probiotic lactobacillus rhamnosus strains with pcr primers based on phage-related sequences. | pcr primers derived from lactobacillus rhamnosus phage lc-nu genome were used to screen the presence of phage-related sequences in lb. rhamnosus strains. several primer pairs derived from structural and replication gene regions of phage lc-nu amplified pcr products of expected sizes from bacterial strains revealing phage-related sequences in 10 of 11 lb. rhamnosus strains. strain-specific pcr primers for three probiotic lb. rhamnosus strains were derived from these phage-related sequences for id ... | 2003 | 12781941 |
| probiotics and prevention of atopic disease: 4-year follow-up of a randomised placebo-controlled trial. | perinatal administration of the probiotic lactobacillus rhamnosus strain gg (atcc 53103), reduces incidence of atopic eczema in at-risk children during the first 2 years of life (infancy). we have therefore assessed persistence of the potential to prevent atopic eczema at 4 years. atopic disease was diagnosed on the basis of a questionnaire and a clinical examination. 14 of 53 children receiving lactobacillus had developed atopic eczema, compared with 25 of 54 receiving placebo (relative risk 0. ... | 2003 | 12788576 |
| consensus-degenerate hybrid oligonucleotide primers for amplification of priming glycosyltransferase genes of the exopolysaccharide locus in strains of the lactobacillus casei group. | a primer design strategy named codehop (consensus-degenerate hybrid oligonucleotide primer) for amplification of distantly related sequences was used to detect the priming glycosyltransferase (gt) gene in strains of the lactobacillus casei group. each hybrid primer consisted of a short 3' degenerate core based on four highly conserved amino acids and a longer 5' consensus clamp region based on six sequences of the priming gt gene products from exopolysaccharide (eps)-producing bacteria. the hybr ... | 2003 | 12788729 |
| immunomodulatory consequences of oral administration of lactobacillus rhamnosus strain gg in healthy volunteers. | probiotic microorganisms, especially lactic acid bacteria, are effective in the treatment of infectious diarrhoeal diseases and experimental colitis. although the mechanisms by which these organisms exert their anti-inflammatory effects are largely unknown, immunomodulating effects are suggested. the objective of this study was to examine the effect of a 5-week oral administration of lactobacillus rhamnosus subspecies gg (lb. gg) on the cellular immune response to intestinal microorganisms in te ... | 2003 | 12800870 |
| lactobacillus strains isolated from danbo cheese as adjunct cultures in a cheese model system. | isolates of non-starter lactic acid bacteria (nslab) from six ripened danbo cheeses of different ages and of different brands were examined. special emphasis was on the genus lactobacillus with the aim of investigating their role in cheese maturation. thirty-three isolates were typed by the pcr-based method, randomly amplified polymorphic dna (rapd). ten rapd types were found and 70% of the isolates were of rapd types found in more than one cheese. the different rapd types were identified to spe ... | 2003 | 12810280 |
| disease-dependent adhesion of lactic acid bacteria to the human intestinal mucosa. | their adhesion to the intestinal mucosa is considered one of the main reasons for the beneficial health effects of specific lactic acid bacteria (lab). however, the influence of disease on the mucosal adhesion is largely unknown. adhesion of selected lab to resected colonic tissue and mucus was determined in patients with three major intestinal diseases (i.e., diverticulitis, rectal carcinoma, and inflammatory bowel disease) and compared to healthy control tissue. all strains were observed to ad ... | 2003 | 12853398 |
| resistance of lactobacillus casei in plastic-composite-support biofilm reactors during liquid membrane extraction and optimization of the lactic acid extraction system. | lactic acid fermentations were performed with plastic-composite-support (pcs) disks in solvent-saturated media with lactobacillus casei subsp. rhamnosus (atcc 11443). the pcs disks contained 50% (w/w) polypropylene, 35% (w/w) ground soybean hulls, 5% (w/w) yeast extract, 5% (w/w) soybean flour, and 5% (w/w) bovine albumin. bioassays were performed by growing l. casei in solvent-saturated media after soaking the pcs disks. eighteen different solvent and carrier combinations were evaluated. overal ... | 2003 | 12889015 |
| effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems. | oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (chx) gluconate-containing mouthwash (chxm) diluted to 0.06% (wt/vol) antimicrobial content. the microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge). chxm caused significant decrease ... | 2003 | 12902270 |
| selective enumeration of lactobacillus delbrueckii ssp. bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacteria, lactobacillus casei, lactobacillus rhamnosus, and propionibacteria. | nineteen bacteriological media were evaluated to assess their suitability to selectively enumerate lactobacillus delbrueckii ssp. bulgaricus, streptococcus thermophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacteria, and propionibacteria. bacteriological media evaluated included streptococcus thermophilus agar, ph modified mrs agar, mrs-vancomycine agar, mrs-bile agar, mrs-nacl agar, mrs-lithium chloride agar, mrs-nnlp (nalidixic acid, neomycin sulfate, ... | 2003 | 12906045 |
| the effect of lactobacillus rhamnosus on enterohemorrhagic escherichia coli infection of human intestinal cells in vitro. | there are many examples of probiotic effects of various lactic bacteria on enteropathogens. in this study, lactobacillus strains (l. rhamnosus, l. gasseri, l. casei and l. plantarum) were tested in an in vitro model of enterohemorrhagic escherichia coli (ehec) infection of a human colon epithelial cell line, c2bbe1. while the adhesion and colonization of ehec was not affected by any of the lactobacillus strains tested, the internalization of ehec into the cell line was markedly suppressed by l. ... | 2003 | 12906100 |