| removal of paralytic shellfish toxins by probiotic lactic acid bacteria. | paralytic shellfish toxins (psts) are non-protein neurotoxins produced by saltwater dinoflagellates and freshwater cyanobacteria. the ability of lactobacillus rhamnosus strains gg and lc-705 (in viable and non-viable forms) to remove psts (saxitoxin (stx), neosaxitoxin (neostx), gonyautoxins 2 and 3 (gtx2/3), c-toxins 1 and 2 (c1/2)) from neutral and acidic solution (ph 7.3 and 2) was examined using hplc. binding decreased in the order of stx ~ neostx > c2 > gtx3 > gtx2 > c1. removal of stx and ... | 2014 | 25046082 |
| a comparative pan-genome perspective of niche-adaptable cell-surface protein phenotypes in lactobacillus rhamnosus. | lactobacillus rhamnosus is a ubiquitously adaptable gram-positive bacterium and as a typical commensal can be recovered from various microbe-accessible bodily orifices and cavities. then again, other isolates are food-borne, with some of these having been long associated with naturally fermented cheeses and yogurts. additionally, because of perceived health benefits to humans and animals, numerous l. rhamnosus strains have been selected for use as so-called probiotics and are often taken in the ... | 2014 | 25032833 |
| lactobacillus rhamnosus gg inhibits the toxic effects of staphylococcus aureus on epidermal keratinocytes. | few studies have evaluated the potential benefits of the topical application of probiotic bacteria or material derived from them. we have investigated whether a probiotic bacterium, lactobacillus rhamnosus gg, can inhibit staphylococcus aureus infection of human primary keratinocytes in culture. when primary human keratinocytes were exposed to s. aureus, only 25% of the keratinocytes remained viable following 24 h of incubation. however, in the presence of 10(8) cfu/ml of live l. rhamnosus gg, t ... | 2014 | 25015889 |
| assessment of synergistic combination potential of probiotic and bacteriophage against antibiotic-resistant staphylococcus aureus exposed to simulated intestinal conditions. | this study was designed to evaluate the combined effect of probiotic lactobacillus rhamnosus and bacteriophage sa11 on the control of antibiotic-sensitive staphylococcus aureus (assa) and antibiotic-resistant s. aureus (arsa) under the simulated intestinal conditions. the survivability of assa and arsa were determined in the simulated phosphate-buffered saline (pbs)-, trypticase soy broth (tsb)-, and milk-based gastric juices adjusted to ph 2.0, 3.0, and 5.0 at 37 °c for 30 min. the inhibitory e ... | 2014 | 25015717 |
| the potential probiotic lactobacillus rhamnosus ctc1679 survives the passage through the gastrointestinal tract and its use as starter culture results in safe nutritionally enhanced fermented sausages. | the human-derived potential probiotic strain lactobacillus rhamnosus ctc1679 was used as a starter culture in reduced fat and sodium low-acid fermented sausages (fuets) to assess its ability to survive through the gastrointestinal tract (git) in a human intervention study consisting of 5 healthy volunteers who consumed 25 g fuet a day for 21 days. faecal samples were analysed during and after consumption. l. rhamnosus ctc1679 produced a transient colonisation of the human git and persisted durin ... | 2014 | 24998181 |
| the influence of shear on the metabolite yield of lactobacillus rhamnosus biofilms. | a tubular recycle bioreactor was employed to ensure homogeneous shear conditions on the biofilm surface. superficial liquid velocities of 0.19 ms(-1), 0.37 ms(-1), 0.55 ms(-1) and 3.65 ms(-1) were used. the highest velocity resulted in negligible cell attachment (chemostat) while the ratio of attached-to-total cell mass escalated as the superficial velocity decreased. the lactic acid yield on glucose increased from 0.75 g g(-1) to 0.90 g g(-1) with declining shear while the corresponding acetoin ... | 2014 | 24994037 |
| effects of lactobacillus rhamnosus gg on hepatic and serum lipid profiles in zebrafish exposed to ethanol. | zebrafish is a powerful tool in pharmacological research and useful to identify new therapies. probiotics can offer therapeutic options in alcoholic liver disease. this study was done in two independent experiments: first, we confirmed the intestinal colonization of probiotic lactobacillus rhamnosus gg (lgg) after ethanol exposure. second, four groups were performed: control (c), probiotic (p), ethanol (e), and probiotic+ethanol (p+e). liver histology, hepatocytes morphometry, hepatic and serum ... | 2014 | 24987799 |
| probiotic properties of pediococcus strains isolated from jeotgals, salted and fermented korean sea-food. | three pediococcus pentosaceus strains were isolated from jeotgals, salted and fermented korean sea-foods, and their probiotic potentials were examined. after 2 h exposure to ph 3.0, p. pentosaceus f66 survived with the survival ratio of 32.6% followed by p. pentosaceus d56 (17.2%) and p. pentosaceus a24 (7.5%). p. pentosaceus f66 also survived better (26.6%) than p. pentosaceus a24 (13.7%) and p. pentosaceus d56 (5.8%) after 2 h exposure to 0.3% bile salts. three strains grew slowly on mrs broth ... | 2014 | 24979684 |
| inhibitory effect of lactobacillus salivarius on streptococcus mutans biofilm formation. | dental caries arises from an imbalance of metabolic activities in dental biofilms developed primarily by streptococcus mutans. this study was conducted to isolate potential oral probiotics with antagonistic activities against s. mutans biofilm formation from lactobacillus salivarius, frequently found in human saliva. we analysed 64 l. salivarius strains and found that two, k35 and k43, significantly inhibited s. mutans biofilm formation with inhibitory activities more pronounced than those of la ... | 2015 | 24961744 |
| epithelial adhesion mediated by pilin spac is required for lactobacillus rhamnosus gg-induced cellular responses. | lactobacillus rhamnosus gg is a widely used probiotic, and the strain's salutary effects on the intestine have been extensively documented. we previously reported that strain gg can modulate inflammatory signaling, as well as epithelial migration and proliferation, by activating nadph oxidase 1-catalyzed generation of reactive oxygen species (ros). however, how strain gg induces these responses is unknown. here, we report that strain gg's probiotic benefits are dependent on the bacterial-epithel ... | 2014 | 24928883 |
| a comparative study of the effect of probiotics on cariogenic biofilm model for preventing dental caries. | dental caries is induced by oral biofilm containing streptococcus mutans. probiotic bacteria were mainly studied for effect on the gastrointestinal tract and have been known to promote human health. however, the information of probiotics for oral health has been lack yet. in this study, we investigated influence of various probiotics on oral bacteria or cariogenic biofilm and evaluated candidate probiotics for dental caries among them. the antimicrobial activity of the spent culture medium of pr ... | 2014 | 24919536 |
| probiotic-associated aspiration pneumonia due to lactobacillus rhamnosus. | lactobacilli are low-virulence, commensal organisms of the gastrointestinal and genitourinary tracts and are commonly used as "probiotic supplements." herein, we describe an episode of respiratory syncytial virus (rsv) bronchiolitis with bacterial superinfection secondary to administration of lactobacillus rhamnosus in an 11-month-old female with trisomy 21. | 2014 | 24899028 |
| activation of epidermal growth factor receptor mediates mucin production stimulated by p40, a lactobacillus rhamnosus gg-derived protein. | the mucus layer coating the gastrointestinal tract serves as the first line of intestinal defense against infection and injury. probiotics promote mucin production by goblet cells in the intestine. p40, a lactobacillus rhamnosus gg-derived soluble protein, has been shown to transactivate the egf receptor (egfr) in intestinal epithelial cells, which is required for inhibition of apoptosis and preservation of barrier function in the colon, thereby ameliorating intestinal injury and colitis. becaus ... | 2014 | 24895124 |
| minireview: gut microbiota: the neglected endocrine organ. | the concept that the gut microbiota serves as a virtual endocrine organ arises from a number of important observations. evidence for a direct role arises from its metabolic capacity to produce and regulate multiple compounds that reach the circulation and act to influence the function of distal organs and systems. for example, metabolism of carbohydrates results in the production of short-chain fatty acids, such as butyrate and propionate, which provide an important source of nutrients as well a ... | 2014 | 24892638 |
| tolerability of a probiotic in subjects with a history of methicillin-resistant staphylococcus aureus colonisation. | methicillin-resistant staphylococcus aureus (mrsa) is a pathogen of major public health importance. colonisation precedes infection; thus reducing mrsa carriage may be of benefit for reducing infection. probiotics represent a novel approach to reducing mrsa carriage. we undertook a pilot feasibility randomised controlled trial of the tolerability and acceptability of probiotics for reducing nasal and intestinal carriage of mrsa. in addition, subjects were screened for vancomycin-resistant entero ... | 2014 | 24889890 |
| modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic lactobacillus rhamnosus crl1505: role of toll-like receptor 2. | the present study evaluated the effect of nasally given lactobacillus rhamnosus crl1505 on the immunocoagulative response during pneumococcal infection in immunocompetent mice. in addition, we aimed to gain insight into the mechanism involved in the immunomodulatory effect of the l. rhamnosus crl1505 strain by evaluating the role of tlr2. results showed that nasally given l. rhamnosus crl1505 effectively regulates inflammation and hemostatic alterations during the pneumococcal infection. immunob ... | 2014 | 24888715 |
| immunobiotic lactobacillus rhamnosus strains differentially modulate antiviral immune response in porcine intestinal epithelial and antigen presenting cells. | previous findings suggested that lactobacillus rhamnosus crl1505 is able to increase resistance of children to intestinal viral infections. however, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized. | 2014 | 24886142 |
| impact of emergency department probiotic treatment of pediatric gastroenteritis: study protocol for the progut (probiotic regimen for outpatient gastroenteritis utility of treatment) randomized controlled trial. | the burden of acute gastroenteritis on children and their families continues to be enormous. probiotics, defined as viable microbial preparations that have a beneficial effect on the health of the host, represent a rapidly expanding field. although clinical trials in children with gastroenteritis have been performed, most have significant flaws, and guidelines do not consistently endorse their use. | 2014 | 24885220 |
| biochemical characterization of the major n-acetylmuramidase from lactobacillus buchneri. | bacterial cell wall hydrolases are essential for peptidoglycan remodelling in regard to bacterial cell growth and division. in this study, peptidoglycan hydrolases (pghs) of different lactobacillus buchneri strains were investigated. first, the genome sequence of l. buchneri cd034 and l. buchneri nrrl b-30929 was analysed in silico for the presence of pghs. of 23 putative pghs with different predicted hydrolytic specificities, the glycosyl hydrolase family 25 domain-containing homologues lbgh25b ... | 2014 | 24858286 |
| viability of the lactobacillus rhamnosus hn001 probiotic strain in swiss- and dutch-type cheese and cheese-like products. | the objective of this study was to determine the viability of the probiotic lactobacillus rhamnosus hn001 in swiss-type and dutch-type cheese and cheese-like products (milk fat is substituted by stearin fraction of palm fat) during manufacture, ripening, and storage. the use of the probiotic l. rhamnosus hn001 in dutch-type cheese and cheese-like products significantly (p = 0.1) changed their chemical composition (protein and fat content) and an insignificant increase (approximately 1.6% in chee ... | 2014 | 24784351 |
| effects of lactobacillus rhamnosus on allergic march model by suppressing th2, th17, and tslp responses via cd4(+)cd25(+)foxp3(+) tregs. | allergic march (am) is characterized by the progression of clinical signs of atopic dermatitis (ad) to allergic asthma or rhinitis, but its pathogenesis is not completely understood. we developed mouse model of am with three 1-week exposures (separated by 2-week interval) to an ova or saline (control) followed by ova challenge. the development of am was confirmed by phenotypes of ad and allergic asthma. increases in il-4, il-17, and thymic stromal lymphopoietin (tslp) responses were associated w ... | 2014 | 24769377 |
| stability of lactobacillus rhamnosus gg in prebiotic edible films. | the concept of prebiotic edible films as effective vehicles for encapsulating probiotic living cells is presented. four soluble fibres (inulin, polydextrose, glucose-oligosaccharides and wheat dextrin) were selected as prebiotic co-components of gelatine based matrices plasticised with glycerol and used for the immobilisation of lactobacillus rhamnosus gg. the addition of prebiotics was associated with a more compact and uniform film structure, with no detectable interspaces or micropores; probi ... | 2014 | 24767059 |
| functional identification of conserved residues involved in lactobacillus rhamnosus strain gg sortase specificity and pilus biogenesis. | in gram-positive bacteria, sortase-dependent pili mediate the adhesion of bacteria to host epithelial cells and play a pivotal role in colonization, host signaling, and biofilm formation. lactobacillus rhamnosus strain gg, a well known probiotic bacterium, also displays on its cell surface mucus-binding pilus structures, along with other lpxtg surface proteins, which are processed by sortases upon specific recognition of a highly conserved lpxtg motif. bioinformatic analysis of all predicted lpx ... | 2014 | 24753244 |
| lactobacillus rhamnosus hn001 decreases the severity of necrotizing enterocolitis in neonatal mice and preterm piglets: evidence in mice for a role of tlr9. | necrotizing enterocolitis (nec) is the leading cause of death from gastrointestinal disease in premature infants and develops partly from an exaggerated intestinal epithelial immune response to indigenous microbes. there has been interest in administering probiotic bacteria to reduce nec severity, yet concerns exist regarding infection risk. mechanisms of probiotic activity in nec are unknown although activation of the microbial dna receptor toll-like receptor-9 (tlr9) has been postulated. we no ... | 2014 | 24742987 |
| orthogonal array deciphering mrs medium requirements for isolated lactobacillus rhamnosus zy with cell properties characterization. | lactobacillus rhamnosus is a well-known lactic acid bacterium (lab), but a new zy strain was isolated for the first time from commercial probiotic powder recently. although many studies have focused on developing cost-effective media for the production of lab, the de man, rogosa and sharpe (mrs) medium is still the most common medium for bioprocesses. the aim of the current study is to decipher the composition of mrs based on a statistical approach, which will allow a higher biomass of lactobaci ... | 2014 | 24721122 |
| the gut-brain axis rewired: adding a functional vagal nicotinic "sensory synapse". | it is generally accepted that intestinal sensory vagal fibers are primary afferent, responding nonsynaptically to luminal stimuli. the gut also contains intrinsic primary afferent neurons (ipans) that respond to luminal stimuli. a psychoactive lactobacillus rhamnosus (jb-1) that affects brain function excites both vagal fibers and ipans. we wondered whether, contrary to its primary afferent designation, the sensory vagus response to jb-1 might depend on ipan to vagal fiber synaptic transmission. ... | 2014 | 24719355 |
| bovine lactoferrin supplementation for prevention of necrotizing enterocolitis in very-low-birth-weight neonates: a randomized clinical trial. | nec is a common and severe complication in premature neonates, particularly those with very-low-birth-weight (vlbw, <1500 g at birth). probiotics including lactobacillus rhamnosus gg (lgg) proved effective in preventing nec in preterm infants in several rcts. | 2014 | 24709463 |
| partial characterization and immunostimulatory activity of exopolysaccharides from lactobacillus rhamnosus kf5. | lactobacillus rhamnosus kf5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (eps) in skim milk. two eps fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography. fraction s1, with a low average molecular weight of 1.36 × 10(4)da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio ... | 2014 | 24702917 |
| dietary supplementation with lactobacilli improves emergency granulopoiesis in protein-malnourished mice and enhances respiratory innate immune response. | this work studied the effect of protein malnutrition on the hemato-immune response to the respiratory challenge with streptococcus pneumoniae and evaluated whether the dietary recovery with a probiotic strain has a beneficial effect in that response. three important conclusions can be inferred from the results presented in this work: a) protein-malnutrition significantly impairs the emergency myelopoiesis induced by the generation of the innate immune response against pneumococcal infection; b) ... | 2014 | 24691464 |
| probiotic attributes of autochthonous lactobacillus rhamnosus strains of human origin. | the study was aimed at evaluating the probiotic potential of indigenous autochthonous lactobacillus rhamnosus strains isolated from infant feces and vaginal mucosa of healthy female. the survival of the selected strains and the two reference strains (l. rhamnosus gg and l. casei actimel) was 67-81 % at ph 2 and 70-80 % after passage through the simulated gastrointestinal fluid. these strains are able to grow in the presence of 4 % bile salt, 10 % nacl, and 0.6 % phenol. the cell surface of l. rh ... | 2014 | 24682879 |
| comparative genome analysis of lactobacillus rhamnosus clinical isolates from initial stages of dental pulp infection: identification of a new exopolysaccharide cluster. | the human oral microbiome has a major role in oral diseases including dental caries. our studies on progression of caries infection through dentin and more recently, the invasion of vital dental pulp, detected lactobacillus rhamnosus in the initial stages of infection of vital pulp tissue. in this study employing current high-throughput next generation sequencing technology we sought to obtain insight into genomic traits of tissue invasive l. rhamnosus, to recognise biomarkers that could provide ... | 2014 | 24632842 |
| development of a locally sustainable functional food based on mutandabota, a traditional food in southern africa. | a probiotic dairy product was developed on the basis of a traditional dish called mutandabota to enable resource-poor populations in southern africa to benefit from a functional food. mutandabota is widely consumed in rural southern africa, making it an ideal food matrix to carry probiotics. first, a process to produce probiotic mutandabota was designed. raw cow milk was boiled and subsequently cooled to ambient temperature (25°c). next, dry pulp from the fruit of the baobab tree (adansonia digi ... | 2014 | 24630646 |
| probiotic administration attenuates myocardial hypertrophy and heart failure after myocardial infarction in the rat. | probiotics are extensively used to promote gastrointestinal health, and emerging evidence suggests that their beneficial properties can extend beyond the local environment of the gut. here, we determined whether oral probiotic administration can alter the progression of postinfarction heart failure. | 2014 | 24625365 |
| probiotics, feeding tolerance, and growth: a comparison between hiv-exposed and unexposed very low birth weight infants. | the aim of this study was to compare the effect of administration of probiotics on feeding tolerance and growth outcomes of hiv-exposed (but uninfected) versus hiv non-exposed preterm infants. the null hypothesis of this study states that there will be no difference in the feeding tolerance and growth outcomes for both probiotic-exposed and unexposed premature very low birth weight infants. | 2014 | 24613436 |
| altered transcription of murine genes induced in the small bowel by administration of probiotic strain lactobacillus rhamnosus hn001. | lactobacillus rhamnosus hn001 is a probiotic strain reported to increase resistance to epithelium-adherent and -invasive intestinal pathogens in experimental animals. to increase understanding of the relationship between strain hn001 and the bowel, transcription of selected genes in the mucosa of the murine small bowel was measured. mice previously naive to lactobacilli (lactobacillus-free mice) were examined after daily exposure to hn001 in drinking water. comparisons were made to results from ... | 2014 | 24584241 |
| insight into the draft genome sequence of human isolate lactobacillus rhamnosus lr231, a bacterium with probiotic potential. | lactobacillus rhamnosus strain lr231 was isolated from the feces of healthy human subjects. it is observed to be a potential probiotic strain, having a broad spectrum of antimicrobial activity against a wide range of human pathogens and food pathogens. here, we provide the 2.59-mb draft genome sequence of l. rhamnosus lr231. | 2014 | 24578273 |
| the influence of probiotics on zebrafish danio rerio innate immunity and hepatic stress. | in this study, the effects of probiotic administration on zebrafish danio rerio intestinal innate immunity and hepatic stress were evaluated. zebrafish adults were treated for 10 days with the probiotic lactobacillus rhamnosus imc 501(®). to assess the effects at the molecular level, the mrna levels of genes involved in the innate immune system, stress response, oxidative stress, and apoptosis were quantified by real-time polymerase chain reaction. an increase of biomarkers related to innate imm ... | 2014 | 24564619 |
| effects of lactobacillus rhamnosus la68 on the immune system of c57bl/6 mice upon oral administration. | probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. in order to examine the immunological aspects of probiotic consumption, lactobacillus rhamnosus la68 was orally administrated using gavage to healthy c57bl/6 mice. after one month splenocytes were isolated, and analysed by flow cytometry. the magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (ifn-γ, il-6, il-10 and il-17) was ... | 2014 | 24559976 |
| draft genome sequence of lactobacillus rhamnosus 2166. | in this report, we present a draft sequence of the genome of lactobacillus rhamnosus strain 2166, a potential novel probiotic. genome annotation and read mapping onto a reference genome of l. rhamnosus strain gg allowed for the identification of the differences and similarities in the genomic contents and gene arrangements of these strains. | 2014 | 24558254 |
| controlled release of lactobacillus rhamnosus biofilm probiotics from alginate-locust bean gum microcapsules. | chitosan-coated alginate microcapsules containing high-density biofilm lactobacillus rhamnosus have been previously shown to exhibit higher freeze drying- and thermal-tolerance than their planktonic counterparts. however, their cell release profile remains poor due to the capsules' susceptibility to the gastric environment. herein the effects of adding locust bean (lb) and xanthan (xt) gums to alginate (agn) capsules on the stress tolerance and cell release profiles in simulated gastrointestinal ... | 2014 | 24528770 |
| spray-dried chestnut extract containing lactobacillus rhamnosus cells as novel ingredient for a probiotic chestnut mousse. | consumers' demand for innovative probiotic products has recently increased. in previous studies, chestnuts were evaluated as substrate for the growth of lactobacilli and chestnut extract was found to enhance acid tolerance of probiotic strains. the main objectives of this study were to evaluate the suitability of chestnut extract as carrier for spray drying of two probiotic lactobacillus rhamnosus strains and to develop a probiotic food chestnut based. | 2014 | 24517262 |
| protection of l. rhamnosus by spray-drying using two prebiotics colloids to enhance the viability. | protection of probiotics by substances considered as prebiotics can be an alternative to increase their viability in the large intestine. the objective of this study was to use two wall materials (native rice starch and inulin) without bonding agent to protect lactobacillus rhamnosus during spray-drying and determine the viability of the microorganism under two storage conditions. for spray-drying conditions tested in this work the product yield with native rice starch (nrs) ranged between 65% a ... | 2014 | 24507301 |
| transcriptomic clues to understand the growth of lactobacillus rhamnosus in cheese. | lactobacillus rhamnosus is a non-starter lactic acid bacterium that plays a significant role during cheese ripening, leading to the formation of flavor. in long-ripened cheeses it persists throughout the whole time of ripening due to its capacity to adapt to changing environmental conditions. the versatile adaptability of l. rhamnosus to different ecosystems has been associated with the capacity to use non-conventional energy sources, regulating different metabolic pathways. however, the molecul ... | 2014 | 24506811 |
| immune response and intestinal permeability in children with acute gastroenteritis treated with lactobacillus rhamnosus gg: a randomized, double-blind, placebo-controlled trial. | probiotics have a possible role in the treatment of pediatric acute gastroenteritis. we report the effect of the probiotic lactobacillus rhamnosus gg (lgg) on intestinal function, immune response, and clinical outcomes in indian children with cryptosporidial or rotavirus diarrhea. | 2014 | 24501384 |
| live and heat-killed lactobacillus rhamnosus gg upregulate gene expression of pro-inflammatory cytokines in 5-fluorouracil-pretreated caco-2 cells. | this study investigates whether post-chemotherapeutic use of live and heat-killed lactobacillus rhamnosus gg can modulate the expression of three pro-inflammatory cytokines in 5-fluorouracil (5-fu)-induced intestinal mucositis in vitro. | 2014 | 24500789 |
| probiotic lactobacillus rhamnosus gr-1 supernatant prevents lipopolysaccharide-induced preterm birth and reduces inflammation in pregnant cd-1 mice. | the objective of this study was to determine the effect of probiotic lactobacillus rhamnosus gr-1 supernatant (gr-1 sn) on lipopolysaccharide-induced preterm birth (ptb) and outputs of cytokines, chemokines, and progesterone in pregnant cd-1 mice. | 2014 | 24486224 |
| lactobacillus gg restoration of the gliadin induced epithelial barrier disruption: the role of cellular polyamines. | celiac disease is characterized by enhanced intestinal paracellular permeability due to alterations of function and expression of tight junction (tj) proteins including zo-1, claudin-1 and occludin. polyamines are pivotal in the control of intestinal barrier function and are also involved in the regulation of intercellular junction proteins. different probiotic strains may inhibit gliadin-induced toxic effects and the lactobacillus rhamnosus gg (l.gg) is effective in the prevention and treatment ... | 2014 | 24483336 |
| cariogenic effects of probiotic lactobacillus rhamnosus gg in a dental biofilm model. | probiotic bacteria have been suggested to inhibit streptococcus mutans (sm) and thus prevent dental caries. however, supporting evidence is weak and probiotic species might be cariogenic themselves. thus, we compared and combined the probiotic lactobacillus rhamnosus gg (lgg) with sm and analysed the resulting mineral loss (δz) in dental tissues. we simulated three biofilm compositions (sm, lgg, sm × lgg), two lesion sites (smooth enamel, dentin cavity) and two nutrition supply frequencies (twic ... | 2014 | 24480927 |
| food matrices and cell conditions influence survival of lactobacillus rhamnosus gg under heat stresses and during storage. | the present study evaluated impact of moisture content and cell conditions on survival of probiotic strain, lactobacillus rhamnosus gg, under lethal heat stresses and during long-term storage using freeze-dried cells and oils as matrices. viable cell counts of freeze-dried l. rhamnosus gg cells suspended in oils had only 1-log-reduction after 5min at 80°c and approximately 3-log-reduction after 20min, while no or very few viable cells were recorded for freeze dried cells suspended in buffer and ... | 2014 | 24480189 |
| lactobacillus rhamnosus gg protects against non-alcoholic fatty liver disease in mice. | experimental evidence revealed that obesity-associated non-alcoholic fatty liver disease (nafld) is linked to changes in intestinal permeability and translocation of bacterial products to the liver. hitherto, no reliable therapy is available except for weight reduction. within this study, we examined the possible effect of the probiotic bacterial strain lactobacillus rhamnosus gg (lgg) as protective agent against experimental nafld in a mouse model. | 2014 | 24475018 |
| probiotics in the prevention of recurrences of bacterial vaginosis. | bacterial vaginosis (bv) is the most common cause of vaginal discharge in women during their fertile years. bv prevalence runs from 10%-50%, in part due to the high rate of recurrence after standard treatment. women with bv may experience a decreased quality of life and are at risk of serious obstetric complications. limited data are available regarding optimal management strategies for preventing recurrence of bv, emphasizing the importance of the availability of a comprehensive source of scien ... | 2014 | 24473986 |
| probiotic lactobacillus rhamnosus inhibits the formation of neutrophil extracellular traps. | neutrophil extracellular traps (nets) are an essential component of the antimicrobial repertoire and represent an effective means by which neutrophils capture, contain, and kill microorganisms. however, the uncontrolled or excessive liberation of nets also damages surrounding cells and can contribute to disease pathophysiology. alterations in the gut microbiota, as well as the presence of local and systemic markers of inflammation, are strongly associated with the manifestation of a spectrum of ... | 2014 | 24465012 |
| prebiotics and probiotics: the prevention and reduction in severity of atopic dermatitis in children. | the purpose of this review was to identify whether supplementation with prebiotics and/or probiotics help prevent the development or reduce the severity of atopic dermatitis in children less than three years of age. since 1997, immunostimulatory supplements, such as prebiotics and probiotics, have been investigated. various supplementations include probiotics (single strain or mix), probiotics with formula, probiotics mix with prebiotics, and prebiotics. in this narrative review, we examined 13 ... | 2014 | 24463205 |
| commensal and probiotic bacteria may prevent nec by maturing intestinal host defenses. | necrotizing enterocolitis (nec) is a devastating disease of prematurity with significant morbidity and mortality. immaturity of intestinal host defenses predisposes the premature infant gut to injury. an abnormal bacterial colonization pattern with a deficiency of commensal bacteria may lead to a further breakdown of these host defense mechanisms, predisposing the infant to nec. the presence of probiotic and commensal bacteria within the gut has been shown to mature the intestinal defense system ... | 2014 | 24440614 |
| lipopolysaccharide-induced profiles of cytokine, chemokine, and growth factors produced by human decidual cells are altered by lactobacillus rhamnosus gr-1 supernatant. | the aim of this study was to assess the effects of bacterial lipopolysaccharide (lps) and lactobacillus rhamnosus gr-1 supernatant (gr-1sn) on secretion profiles of cytokines, chemokines, and growth factors from primary cultures of human decidual cells. lipopolysaccharide significantly increased the output of proinflammatory cytokines (interleukin [il]-1b, il-2, il-6, il-12p70, il-15, il-17a, interferon gamma [ifn-γ], and tumor necrosis factor [tnf]); anti-inflammatory cytokines (il-1rn, il-4, i ... | 2014 | 24429676 |
| immunobiotic lactobacilli reduce viral-associated pulmonary damage through the modulation of inflammation-coagulation interactions. | the exacerbated disease due to immune- and coagulative-mediated pulmonary injury during acute respiratory viruses infection results in severe morbidity and mortality. identifying novel approaches to modulate virus-induced inflammation-coagulation interactions could be important alternatives for treating acute respiratory viruses infections. in this study we investigated the effect of the probiotic strain lactobacillus rhamnosus crl1505 on lung tlr3-mediated inflammation, and its ability to modul ... | 2014 | 24394565 |
| probiotic supplementation promotes calcification in danio rerio larvae: a molecular study. | a growing number of studies have been showing that dietary probiotics can exert beneficial health effects in both humans and animals. we previously demonstrated that dietary supplementation with lactobacillus rhamnosus - a component of the human gut microflora - enhances reproduction, larval development, and the biomineralization process in danio rerio (zebrafish). the aim of this study was to identify the pathways affected by l. rhamnosus during zebrafish larval development. our morphological a ... | 2013 | 24358259 |
| protective effects of probiotic lactobacillus rhamnosus imc501 in mice treated with phip. | the aim of the present study was to investigate the antigenotoxic properties of the probiotic lactobacillus rhamnosus imc501; dna damage was induced by one representative food mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip). mice were treated orally with suspensions of lactobacilli for 10 days before administration of food mutagen. during the treatment, the abundance of lactobacilli in feces, as assessed by qpcr analysis, increased, whereas β-glucuronidase and n-acetyl-β-glucosam ... | 2014 | 24346468 |
| the effect of alginate and chitosan concentrations on some properties of chitosan-coated alginate beads and survivability of encapsulated lactobacillus rhamnosus in simulated gastrointestinal conditions and during heat processing. | in this study, chitosan-coated alginate beads were produced with different concentrations of chitosan and alginate to evaluate the survival of encapsulated lactobacillus rhamnosus gg during exposure to adverse conditions in gastrointestinal simulated juice and heat processing. | 2014 | 24343670 |
| effect of lactobacillus rhamnosus cgmcc1.3724 supplementation on weight loss and maintenance in obese men and women. | the present study investigated the impact of a lactobacillus rhamnosus cgmcc1.3724 (lpr) supplementation on weight loss and maintenance in obese men and women over 24 weeks. in a double-blind, placebo-controlled, randomised trial, each subject consumed two capsules per d of either a placebo or a lpr formulation (1.6 × 10(8) colony-forming units of lpr/capsule with oligofructose and inulin). each group was submitted to moderate energy restriction for the first 12 weeks followed by 12 weeks of wei ... | 2014 | 24299712 |
| single-cell force spectroscopy of pili-mediated adhesion. | although bacterial pili are known to mediate cell adhesion to a variety of substrates, the molecular interactions behind this process are poorly understood. we report the direct measurement of the forces guiding pili-mediated adhesion, focusing on the medically important probiotic bacterium lactobacillus rhamnosus gg (lgg). using non-invasive single-cell force spectroscopy (scfs), we quantify the adhesion forces between individual bacteria and biotic (mucin, intestinal cells) or abiotic (hydroph ... | 2014 | 24296882 |
| engineered lactobacillus rhamnosus gg expressing igg-binding domains of protein g: capture of hyperimmune bovine colostrum antibodies and protection against diarrhea in a mouse pup rotavirus infection model. | rotavirus-induced diarrhea causes more than 500,000 deaths annually in the world, and although vaccines are being made available, new effective treatment strategies should still be considered. purified antibodies derived from hyperimmune bovine colostrum (hbc), from cows immunized with rotavirus, were previously used for treatment of rotavirus diarrhea in children. a combination of hbc antibodies and a probiotic strain of lactobacillus (l. rhamnosus gg) was also found to be more effective than h ... | 2014 | 24291196 |
| lactobacillus rhamnosus gg on rotavirus-induced injury of ileal epithelium in gnotobiotic pigs. | the aim of this study was to study the effect of continued lactobacillus rhamnosus gg strain (lgg) feeding on rotavirus gastroenteritis in the gnotobiotic (gn) pig model of virulent human rotavirus (hrv) infection. | 2013 | 24280990 |
| mn(2+) and mg(2+) synergistically enhanced lactic acid production by lactobacillus rhamnosus ftdc 8313 via affecting different stages of the hexose monophosphate pathway. | the study aimed to evaluate the effects of mn(2+) and mg(2+) on lactic acid production using response surface methodology and to further study their effects on interactions between the enzymes and substrates along the hexose monophosphate pathway using a molecular modelling approach. | 2014 | 24267975 |
| obsessive-compulsive-like behaviors in house mice are attenuated by a probiotic (lactobacillus rhamnosus gg). | two experiments examined probiotic pretreatment (lactobacillus rhamnosus gg) on obsessive-compulsive disorder (ocd)-like behavior induction by ru 24969 in balb/cj house mice. in the first experiment, two groups were defined by their daily pretreatment by oral gavage of either (a) l. rhamnosus (1×10⁹ cfu/day) or (b) the saline vehicle. both a 2- and 4-week probiotic pretreatment attenuated ocd-like behavior induction (increased perseverative open-field locomotion, stereotypic turning, and marble ... | 2014 | 24257436 |
| applications of lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications. | this study was aimed at investigating the antioxidant, whitening, and moisture-retention properties of lactobacillus rhamnosus spent culture supernatant (lr-scs) in cosmetic applications. results reveal that lr-scs effectively and gradually scavenges 1,1-diphenyl-2-picrylhydrazyl as well as 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cations, and increases reducing power in a dose-dependent manner. additionally, lr-scs can also suppress tyrosinase activity in vitro and effecti ... | 2013 | 24248144 |
| lactobacillus rhamnosus gg improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory t cells. | recent clinical trials show lactobacillus rhamnosus gg (lgg) administration in critical illness has the potential to reduce nosocomial infections and improve clinical outcome. however, the mechanism(s) of lgg-mediated benefit following illness and injury remain elusive. | 2013 | 24240593 |
| growth of infants fed formula supplemented with bifidobacterium lactis bb12 or lactobacillus gg: a systematic review of randomized controlled trials. | growth is an essential outcome measure for evaluating the safety of any new ingredients, including probiotics, added to infant formulae. the aim of this systematic review was to determine the effects of supplementation of infant formulae with bifidobacterium lactis bb12 (b lactis) and/or lactobacillus rhamnosus gg (lgg) compared with unsupplemented formula on the growth of healthy infants. | 2013 | 24215626 |
| perinatal outcomes of prenatal probiotic and prebiotic administration: an integrative review. | the purpose of this integrative review was to identify, critique, and synthesize the maternal and neonatal evidence on the prenatal use of probiotics and prebiotics to inform perinatal health professionals. a comprehensive literature search resulted in 37 studies of prenatal probiotics and 1 on antepartal prebiotics published from 1990 through 2011 that reported maternal, fetal, and/or neonatal outcomes. the methodologic quality of the studies reviewed was high, although investigators used diffe ... | 2015 | 24164813 |
| submerged fermentation of lactobacillus rhamnosus ys9 for γ-aminobutyric acid (gaba) production. | γ-aminobutyric acid (gaba) is a major inhibitory neurotransmitter in central nervous system, and its application in drugs and functional foods has attracted great attention. to enhance production of γ-aminobutyric acid, lactobacillus rhamnosus ys9, a strain isolated from chinese traditional fermented food pickled vegetable, was grown under submerged fermentation. its cultivation conditions were investigated. when culture ph condition was adjusted to the optimal ph of glutamate decarboxylase acti ... | 2013 | 24159304 |
| prebiotic and probiotic supplementation prevents rhinovirus infections in preterm infants: a randomized, placebo-controlled trial. | simple and safe strategies for the prevention of viral respiratory tract infections (rtis) are needed. | 2014 | 24131826 |
| lactobacillus rhamnosus crl1505 beneficially modulates the immuno-coagulative response after pneumococcal infection in immunocompromised malnourished mice. | this work evaluated the effect of orally or nasally administered lactobacillus rhamnosus crl1505 on the resistance of immunocompromised protein-malnourished mice to pneumococcal infection. in particular, we aimed to gain knowledge of the mechanism involved in the immunomodulatory effect of l. rhamnosus crl1505 in malnourished hosts by evaluating its impact on the immuno-coagulative response. malnutrition significantly increased lung tissue damage caused by streptococcus pneumoniae infection. lun ... | 2013 | 24102222 |
| kinetic model-based feed-forward controlled fed-batch fermentation of lactobacillus rhamnosus for the production of lactic acid from arabic date juice. | arabic date is overproduced in arabic countries such as saudi arabia and iraq and is mostly composed of sugars (70-80 wt%). here we developed a fed-batch fermentation process by using a kinetic model for the efficient production of lactic acid to a high concentration from arabic date juice. first, a kinetic model of lactobacillus rhamnosus grown on date juice in batch fermentation was constructed in excel so that the estimation of parameters and simulation of the model can be easily performed. t ... | 2014 | 24100793 |
| purification, crystallization and preliminary crystallographic analysis of the spaa backbone-pilin subunit from probiotic lactobacillus rhamnosus gg. | lactobacillus rhamnosus gg, a widely used gram-positive probiotic strain, is clinically well known for its perceived health-promoting effects. it has recently been shown to display proteinaceous pilus fibres (called spacba) on its cell surface. structurally, spacba pili possess a characteristic three-pilin polymerized architecture, with repeating spaa major pilins that form the backbone and two types of minor subunits (spab and spac). in this study, recombinant spaa protein was purified, charact ... | 2013 | 24100577 |
| lactobacilli and bifidobacteria promote immune homeostasis by modulating innate immune responses to human rotavirus in neonatal gnotobiotic pigs. | the effects of co-colonization with lactobacillus rhamnosus gg (lgg) and bifidobacterium lactis bb12 (bb12) on 3-dose vaccination with attenuated hrv and challenge with virulent human rotavirus (virhrv) were assessed in 4 groups of gnotobiotic (gn) pigs: pro+vac (probiotic-colonized/vaccinated), vac (vaccinated), pro (probiotic-colonized, non-vaccinated) and control (non-colonized, non-vaccinated). subsets of pigs were euthanized pre- [post-challenge day (pcd) 0] and post (pcd7)-virhrv challenge ... | 2013 | 24098572 |
| bifidogenic and antioxidant activity of exopolysaccharides produced by lactobacillus rhamnosus e/n cultivated on different carbon sources. | lactobacillus rhamnosus e/n is a probiotic bacterium, which synthesizes exopolysaccharides (eps) with significant bifidogenic and antioxidant activities. the sugar composition of the epss produced depended on carbohydrates used as a carbon source in the growth media. five bifidobacterium strains were tested in vitro for their ability to utilize all the epss studied. the highest bifidogenic activity was revealed by epss obtained from lactobacillus cultures supplemented with gal, lac, and mal as t ... | 2013 | 24053021 |
| a lactobacillus rhamnosus gg-derived soluble protein, p40, stimulates ligand release from intestinal epithelial cells to transactivate epidermal growth factor receptor. | p40, a lactobacillus rhamnosus gg (lgg)-derived soluble protein, ameliorates intestinal injury and colitis, reduces apoptosis, and preserves barrier function by transactivation of the egf receptor (egfr) in intestinal epithelial cells. the aim of this study is to determine the mechanisms by which p40 transactivates the egfr in intestinal epithelial cells. here we show that p40-conditioned medium activates egfr in young adult mouse colon epithelial cells and human colonic epithelial cell line, t8 ... | 2013 | 24043629 |
| crystal structure and substrate specificity of d-galactose-6-phosphate isomerase complexed with substrates. | d-galactose-6-phosphate isomerase from lactobacillus rhamnosus (lacab; ec 5.3.1.26), which is encoded by the tagatose-6-phosphate pathway gene cluster (lacabcd), catalyzes the isomerization of d-galactose-6-phosphate to d-tagatose-6-phosphate during lactose catabolism and is used to produce rare sugars as low-calorie natural sweeteners. the crystal structures of lacab and its complex with d-tagatose-6-phosphate revealed that lacab is a homotetramer of laca and lacb subunits, with a structure sim ... | 2013 | 24015281 |
| biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance. | microcapsules containing high-density biofilm-like lactobacillus rhamnosus probiotics, in place of planktonic cells, are developed in order to enhance the cell viability upon exposures to stresses commonly encountered during food lifecycle (i.e., heating, freeze-drying, refrigerated storage, and acid). the high-density (hd) capsules are prepared by in situ cultivation of the planktonic cells in the confined space of polysaccharide-based capsules (i.e., chitosan-coated alginate and carrageenan ca ... | 2013 | 23985031 |
| comparative genomic and functional analysis of 100 lactobacillus rhamnosus strains and their comparison with strain gg. | lactobacillus rhamnosus is a lactic acid bacterium that is found in a large variety of ecological habitats, including artisanal and industrial dairy products, the oral cavity, intestinal tract or vagina. to gain insights into the genetic complexity and ecological versatility of the species l. rhamnosus, we examined the genomes and phenotypes of 100 l. rhamnosus strains isolated from diverse sources. the genomes of 100 l. rhamnosus strains were mapped onto the l. rhamnosus gg reference genome. th ... | 2013 | 23966868 |
| draft genome sequence of lactobacillus rhamnosus crl1505, an immunobiotic strain used in social food programs in argentina. | we report the draft genome sequence of the probiotic lactobacillus rhamnosus strain crl1505. this new probiotic strain has been included into official nutritional programs in argentina. the draft genome sequence is composed of 3,417,633 bp with 3,327 coding sequences. | 2013 | 23950129 |
| nasally administered lactobacillus rhamnosus strains differentially modulate respiratory antiviral immune responses and induce protection against respiratory syncytial virus infection. | some studies have shown that nasally administered immunobiotics had the potential to improve the outcome of influenza virus infection. however, the capacity of immunobiotics to improve protection against respiratory syncytial virus (rsv) infection was not investigated before. | 2013 | 23947615 |
| probiotic lactobacillus rhamnosus gg mono-association suppresses human rotavirus-induced autophagy in the gnotobiotic piglet intestine. | human rotavirus (hrv) is the most important cause of severe diarrhea in infants and young children. probiotic lactobacillus rhamnosus gg (lgg) reduces rotavirus infection and diarrhea. however, the molecular mechanisms of lgg-mediated protection from rotavirus infection are poorly understood. autophagy plays an essential role in responses to microbial pathogens. however, the role of autophagy in hrv infection and lgg treatment is unknown. we hypothesize that rotavirus gastroenteritis activates a ... | 2013 | 23924832 |
| divergent immunomodulating effects of probiotics on t cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model. | rotaviruses (rvs) are a leading cause of childhood diarrhea. current oral vaccines are not effective in impoverished countries where the vaccine is needed most. therefore, alternative affordable strategies are urgently needed. probiotics can alleviate diarrhea in children and enhance specific systemic and mucosal ab responses, but the t cell responses are undefined. in this study, we elucidated the t cell and cytokine responses to attenuated human rv (atthrv) and virulent human rv (hrv) in gnoto ... | 2013 | 23918983 |
| water sorption properties, molecular mobility and probiotic survival in freeze dried protein-carbohydrate matrices. | the moisture uptake and molecular mobility of freeze-dried powders containing whey protein isolate-carbohydrate matrices (1wpi:2maltodextrin; 1wpi:1maltodextrin:1d-glucose; and 1wpi:1maltodextrin:1l-glucose) and encapsulated lactobacillus rhamnosus gg (lgg) in these matrices were investigated at 25 °c and 33% and 70% relative humidity (rh). the inactivation rate constant for probiotics in freeze-dried matrices were positively correlated (r(2) = 0.98) to moisture uptake and molecular mobility mea ... | 2013 | 23851914 |
| immunobiotic lactobacillus rhamnosus improves resistance of infant mice against respiratory syncytial virus infection. | previously we showed that orally administered lactobacillus rhamnosus crl1505 beneficially regulated the balance between pro- and anti-inflammatory mediators in the lungs of poly(i:c)-challenged mice, allowing an effective inflammatory response against the tlr3/rig-i agonist but at the same time reducing tissue damage. the aim of the present study was to investigate whether oral administration of the crl1505 strain was able to improve resistance against respiratory syncytial virus (rsv) infectio ... | 2013 | 23838113 |
| effect of a mixture of bovine milk oligosaccharides, lactobacillus rhamnosus ncc4007 and long-chain polyunsaturated fatty acids on catch-up growth of intra-uterine growth-restricted rats. | to investigate the effect of a nutritional mixture (bovine milk oligosaccharides, lactobacillus rhamnosus ncc4007, arachidonic and docosahexaenoic acid) on growth of intrauterine growth-restricted (iugr) rats. | 2014 | 23834457 |
| the role of vaginal lactobacillus rhamnosus (normogin®) in preventing bacterial vaginosis in women with history of recurrences, undergoing surgical menopause: a prospective pilot study. | bacterial vaginosis (bv), a poly-microbial clinical syndrome, is the most common cause of vaginal symptoms among women. the recurrence rate of bv is up to 30% after traditional antimicrobial therapy. lactobacillus rhamnosus vaginal tablets have demonstrated to be a reliable topical effective and safe treatment to reduce the bv recurrence rate. | 2013 | 23740456 |
| characterization of mutual relations between geotrichum candidum and lactobacillus rhamnosus gg in milk: a quantitative approach. | the growth interactions between geotrichum candidum and lactobacillus rhamnosus gg were studied in milk. the effect of temperature on the growth rate of the fungus was modelled using the cardinal temperature model with inflection. the secondary modelling was applied also on the other data set containing the growth rates of g. candidum in co-culture with a commercial starter culture. the low temperature in combination with l. rhamnosus gg in co-culture showed the most negative effect on the growt ... | 2014 | 23733814 |
| effects of lactobacillus rhamnosus on asthma with an adoptive transfer of dendritic cells in mice. | this study was designed to investigate whether the protective effects of lactobacillus rhamnosus (lcr35) on allergic asthma are associated with the adoptive transfer of dendritic cells (dcs) and regulatory t cells (tregs), using a mouse experimental model of asthma. | 2013 | 23725296 |
| using recombinant lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic lactobacillus rhamnosus gg. | primarily arising from their well understood beneficial health effects, many lactobacilli strains are considered good candidates for use as probiotics in humans and animals. lactobacillar probiosis can itself be best typified by the lactobacillus rhamnosus gg strain, which, with its well-documented clinical benefits, has emerged as one of the most widely used probiotics in the food and health-supplement industries. even so, many facets of its molecular mechanisms and limitations as a beneficial ... | 2013 | 23691212 |
| effect of nutrient supplementation on atopic dermatitis in children: a systematic review of probiotics, prebiotics, formula, and fatty acids. | to identify whether nutrient supplementation with probiotics, prebiotics, formula, or fatty acids prevents the development of atopic dermatitis (ad) or reduces the severity of ad in newborns to children younger than 3 years. | 2013 | 23682371 |
| lactobacillus rhamnosus gg supplementation for preventing respiratory infections in children: a meta-analysis of randomized, placebo-controlled trials. | to systematically review the effectiveness of administering lactobacillus rhamnosus gg (lgg) for preventing respiratory infections in children. | 2013 | 23665598 |
| associations between the human intestinal microbiota, lactobacillus rhamnosus gg and serum lipids indicated by integrated analysis of high-throughput profiling data. | accumulating evidence indicates that the intestinal microbiota regulates our physiology and metabolism. bacteria marketed as probiotics confer health benefits that may arise from their ability to affect the microbiota. here high-throughput screening of the intestinal microbiota was carried out and integrated with serum lipidomic profiling data to study the impact of probiotic intervention on the intestinal ecosystem, and to explore the associations between the intestinal bacteria and serum lipid ... | 2013 | 23638368 |
| protective effects of lactobacillus rhamnosus gg against human rotavirus-induced diarrhoea in a neonatal mouse model. | group a human rotaviruses (rv) are a leading cause of severe dehydration and gastroenteritis in infants and young children. a large body of evidence suggests that lactobacillus rhamnosus gg (lgg) has an effect on the incidence and severity of acute rv-induced diarrhoea; however, the timing and dosage of lgg treatment remains controversial. in the present study, a neonatal mouse model with human rv-induced diarrhoea was set up and the pathophysiological characteristics of the animals were examine ... | 2013 | 23620181 |
| lactobacillus rhamnosus gg reduces hepatic tnfα production and inflammation in chronic alcohol-induced liver injury. | the therapeutic effects of probiotic treatment in alcoholic liver disease (ald) have been studied in both patients and experimental animal models. although the precise mechanisms of the pathogenesis of ald are not fully understood, gut-derived endotoxin has been postulated to play a crucial role in hepatic inflammation. previous studies have demonstrated that probiotic therapy reduces circulating endotoxin derived from intestinal gram-negative bacteria in ald. in this study, we investigated the ... | 2013 | 23618528 |
| probiotics and prebiotics in preventing food allergy and eczema. | to describe the current literature on clinical trials of probiotics for eczema and food allergy prevention in view of recent new approaches and long-term follow-ups. | 2013 | 23594506 |
| formula selection for management of children with cow's milk allergy influences the rate of acquisition of tolerance: a prospective multicenter study. | to prospectively evaluate the effect of different dietary management strategies on the rate of acquisition of tolerance in children with cow's milk allergy (cma). | 2013 | 23582142 |
| inhibition of streptococcus pneumoniae adherence to human epithelial cells in vitro by the probiotic lactobacillus rhamnosus gg. | colonization of the nasopharynx by streptococcus pneumoniae is considered a prerequisite for pneumococcal infections such as pneumonia and otitis media. probiotic bacteria can influence disease outcomes through various mechanisms, including inhibition of pathogen colonization. here, we examine the effect of the probiotic lactobacillus rhamnosus gg (lgg) on s. pneumoniae colonization of human epithelial cells using an in vitro model. we investigated the effects of lgg administered before, at the ... | 2013 | 23561014 |
| the effect of american cranberry (vaccinium macrocarpon) constituents on the growth inhibition, membrane integrity, and injury of escherichia coli o157:h7 and listeria monocytogenes in comparison to lactobacillus rhamnosus. | the antimicrobial properties of the american cranberry were studied against escherichia coli o157:h7, listeria monocytogenes, and lactobacillus rhamnosus to determine the effects on growth inhibition, membrane permeability, and injury. cranberry powder was separated using a c-18 sep-pak cartridge into sugars plus organic acids (f1), monomeric phenolics (f2), and anthocyanins plus proanthocyanidins (f3). fraction 3 was further separated into anthocyanins (f4) and proanthocyanidins (f5) using an l ... | 2013 | 23541202 |