| lactobacillus rhamnosus gg spac pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates. | lactobacillus rhamnosus gg (lgg) is a well-established probiotic strain. the beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. the pilin spac subunit, located within the spa pili structure, is the most well studied lgg adhesion factor. however, the binding epitopes of spac remain largely unknown. the aim of this study was to evaluate the binding properties of s ... | 2016 | 26434750 |
| efficient magnesium lactate production with in situ product removal by crystallization. | in this paper, attempts were made to develop an in situ product removal process for magnesium lactate production based on crystallization. the crystallization was conducted at 42°c without seed crystal addition. the product concentration, productivity and yield of fermentation coupled with in situ product removal (ispr) reached 143 g l(-1), 2.41 g l(-1)h(-1) and 94.3%. in four cycles of crystallization, the average reuse rate of fermentation medium and removal rate of product reached 64.0% and 7 ... | 2015 | 26433791 |
| short-chain fatty acids produced by synbiotic mixtures in skim milk differentially regulate proliferation and cytokine production in peripheral blood mononuclear cells. | short chain fatty acids (scfas) are major products of prebiotic fermentation and confer human health benefits such as immune-regulation. in this study, reconstituted skim milk supplemented with prebiotics (rsmp) including inulin, hi-maize or β-glucan was fermented by probiotic strains of lactobacillus spp. and bifidobacteria spp. after 24 h of fermentation, probiotics growth and scfas production were investigated and the produced scfas were extracted. inulin and lactobacillus rhamnosus gg atcc 5 ... | 2015 | 26398897 |
| lactobacillus rhamnosus gg-supplemented formula expands butyrate-producing bacterial strains in food allergic infants. | dietary intervention with extensively hydrolyzed casein formula supplemented with lactobacillus rhamnosus gg (ehcf+lgg) accelerates tolerance acquisition in infants with cow's milk allergy (cma). we examined whether this effect is attributable, at least in part, to an influence on the gut microbiota. fecal samples from healthy controls (n=20) and from cma infants (n=19) before and after treatment with ehcf with (n=12) and without (n=7) supplementation with lgg were compared by 16s rrna-based ope ... | 2016 | 26394008 |
| feeding probiotic lactobacillus rhamnosus (mtcc 5897) fermented milk to suckling mothers alleviates ovalbumin-induced allergic sensitisation in mice offspring. | the neonatal period is often polarised to t helper (th2) response at the time of birth, predisposing offspring to allergic disorders. passive immunity through the mother's milk is critical for immune system development of newborns. probiotics have been proposed to harmonise th1/th2 imbalance in allergic conditions in adults. in the present study, the anti-allergic effects of feeding probiotic lactobacillus rhamnosus-fermented milk (pfm) either to dams during the suckling period or to their offsp ... | 2015 | 26330132 |
| influence of milk fermented with lactobacillus rhamnosus ncdc 17 alone and in combination with herbal ingredients on diet induced adiposity and related gene expression in c57bl/6j mice. | obesity has become a major health problem in developed countries and is rapidly catching up in the developing world due to changes in their life style. dietary incorporation of functional foods, including probiotic fermented milk and herbal ingredients, is being tried to ameliorate metabolic disorders. in the present study, the effect of dietary supplementation of a probiotic (lactobacillus rhamnosus ncdc 17) fermented milk alone or either of the herbal preparations (aloe vera/gymnema sylvestre ... | 2015 | 26327356 |
| effect of live and inactivated lactobacillus rhamnosus gg on experimentally induced rhinovirus colds: randomised, double blind, placebo-controlled pilot trial. | the aim of this work was to investigate the usability of an experimental rhinovirus model in probiotic trials aiming to assess effectiveness in viral infections, and to provide preliminary data of live and inactivated probiotic lactobacillus rhamnosus gg for larger-scale trials utilising the model. 59 subjects were randomised to receive 100 ml of fruit juice supplemented with 10(9) cfu of live or heat-inactivated (by spray-drying) l. rhamnosus gg or control juice daily for six weeks. after three ... | 2015 | 26322544 |
| antigenotoxic and antimutagenic activities of probiotic lactobacillus rhamnosus vc against n-methyl-n'-nitro-n-nitrosoguanidine. | the present study provides experimental evidence of in vivo reduction of genotoxic and mutagenic activities of potent carcinogen n-methyl-n'-nitro-n-nitrosoguanidine (mnng) by the strain lactobacillus rhamnosus vc. in vitro studies revealed that coincubation of mnng with viable cells of l. rhamnosus vc resulted in the detoxification of the parent compound accompanied with reduction in genotoxicity (69%) and mutagenicity (61%) as evaluated by sos-chromotest and ames test, respectively. oral feedi ... | 2015 | 26312410 |
| randomized controlled trial to determine the impact of probiotic administration on colonization with multidrug-resistant organisms in critically ill patients. | this was a randomized controlled pilot study of lactobacillus rhamnosus gg versus standard of care to prevent gastrointestinal multidrug-resistant organism colonization in intensive care unit patients. among 70 subjects, there were no significant differences in acquisition or loss of any multidrug-resistant organisms (p>.05) and no probiotic-associated adverse events. | 2015 | 26308946 |
| [lactobacillus rhamnosus gg inhibits cronobacter-induced meningitis in neonatal rats]. | to investigate the inhibitory effect of lactobacillus rhamnosus gg ( lgg) against cronobacter-induced meningitis in neonatal rats. | 2015 | 26277500 |
| effect of the probiotic lactobacillus rhamnosus on the expression of genes involved in european eel spermatogenesis. | positive effects of probiotics on fish reproduction have been reported in several species. in the present study, 40 male european eels were weekly treated with recombinant hcg for 9 weeks and with three different concentrations (10(3), 10(5), and 10(6) cfu/ml) of probiotic lactobacillus rhamnosus imc 501 (sinbyotec, italy). the probiotics were daily added to the water from the sixth week of the hcg treatment. males from the treated and control groups were sacrificed after 1, 2, and 3 weeks of pr ... | 2015 | 26271165 |
| symbiotic maple saps minimize disruption of the mice intestinal microbiota after oral antibiotic administration. | this study was undertaken to evaluate the in vivo impact of new symbiotic products based on liquid maple sap or its concentrate. sap and concentrate, with or without inulin (2%), were inoculated with bifidobacterium lactis bb12 and lactobacillus rhamnosus gg valio at initial counts of 2-4 × 10(8) cfu ml(-1). the experiments started with intra-gastric administration of antibiotic (kanamycin 40 mg in 0.1 cc) (to induce microbiota disturbance and/or diarrhea) to 3-to-5-week-old c57bl/6 female mice ... | 2015 | 26218660 |
| impacts of ph-mediated eps structure on probiotic bacterial pili-whey proteins interactions. | probiotic bacteria are routinely incorporated into dairy foods because of the health benefits they can provide when consumed. in this work, the marked ph-dependence of the pili/eps organization at the outer surface of lactobacillus rhamnosus gg is characterized in detail by single cell force microscopy and cell electrophoretic mobility measurements analyzed according to formalisms for nanomechanical contact and soft particle electrokinetics, respectively. at ph 6.8, lgg pili are easily accessibl ... | 2015 | 26209966 |
| characterization of an antibacterial compound, 2-hydroxyl indole-3-propanamide, produced by lactic acid bacteria isolated from fermented batter. | lactic acid bacteria are known to produce numerous antimicrobial compounds that are active against various pathogens. here, we have purified and characterized a novel low-molecular-weight (lmw) antimicrobial compound produced by lactobacillus and pediococcus isolated from fermented idly and uttapam batter. the lmw compound was extracted from cell-free supernatant using ice-cold acetone, purified by gel permeation and hydrophobic interaction chromatography. it exhibited antimicrobial activity aga ... | 2015 | 26201479 |
| wheat straw degradation and production of alternative substrates for nitrogenase of rhodobacter sphaeroides. | cellulose is a major component of plant biomass and could be applied in the production of biofuels, especially bioethanol. an alternative approach is production of a clean fuel - hydrogen from cellulosic biomass. in this paper an innovatory model of cellulosic waste degradation has been proposed to verify the possibility of utilization of cellulose derivatives by purple non-sulfur bacteria. the concept is based on a two-step process of wheat straw conversion by bacteria in order to obtain an org ... | 2015 | 26192769 |
| pilins in gram-positive bacteria: a structural perspective. | pilins or fimbrilins are a class of proteins found in bacterial surface pilus, a hair-like surface appendage. both the gram-negative and -positive bacteria produce pilins to assemble pili on their cell-surface for different purposes including adherence, twitching motility, conjugation, immunomodulation, biofilm formation, and electron transfer. immunogenic properties of the pilins make them attractive vaccine candidates. the polymerized pilins play a key role in the initiation of host adhesion, ... | 2015 | 26178080 |
| restoring vaginal microbiota: biological control of bacterial vaginosis. a prospective case-control study using lactobacillus rhamnosus bmx 54 as adjuvant treatment against bacterial vaginosis. | bacterial vaginosis (bv) is the most prevalent lower genital tract infection in reproductive-age women worldwide. bv is an ecological disorder of the vaginal microbiota characterized microbiologically by replacement of the lactobacilli, predominant vaginal microbiota. it is characterized by a high rate of relapse in sexual active women, and these patients show three or more relapses each year. a healthy vagina is characterized by hydrogen peroxide and acid-producing lactobacilli, which are cruci ... | 2016 | 26142892 |
| simultaneous discrimination of species and strains in lactobacillus rhamnosus using species-specific pcr combined with multiplex mini-sequencing technology. | this study described the use of species-specific pcr in combination with snapshot mini-sequencing to achieve species identification and strain differentiation in lactobacillus rhamnosus. to develop species-specific pcr and strain subtyping primers, the dnaj gene was used as a target, and its corresponding sequences were analyzed both in lb. rhamnosus and in a subset of its phylogenetically closest species. the results indicated that the species-specific primer pair was indeed specific for lb. rh ... | 2015 | 26141113 |
| probiotic lactobacilli: a potential prophylactic treatment for reducing pesticide absorption in humans and wildlife. | numerous pesticides are used in agriculture, gardening, and wildlife-control. despite their intended toxicity to pests, these compounds can also cause harm to wildlife and humans due to their ability to potentially bioaccumulate, leach into soils, and persist in the environment. humans and animals are commonly exposed to these compounds through agricultural practices and consumption of contaminated foods and water. pesticides can cause a range of adverse effects in humans ranging from minor irri ... | 2015 | 26123785 |
| influence of gastrointestinal stress on autoinducer-2 activity of two lactobacillus species. | quorum sensing is a bacterial communication signalling system that regulates the expression of certain target genes with autoinducers in a cell density-dependent manner. the universal luxs-mediated quorum sensing using the autoinducer-2 (ai-2) signal is present in a wide variety of bacteria with only sparse information on probiotic lactobacilli. effective probiotics should exhibit tolerance and adaptation to stress conditions typical of the git. adhesion to human intestinal epithelial cells and ... | 2015 | 26092949 |
| an exopolysaccharide-deficient mutant of lactobacillus rhamnosus gg efficiently displays a protective llama antibody fragment against rotavirus on its surface. | rotavirus is the leading cause of infantile diarrhea in developing countries, where it causes a high number of deaths among infants. two vaccines are available, being highly effective in developed countries although markedly less efficient in developing countries. as a complementary treatment to the vaccines, a lactobacillus strain producing an anti-rotavirus antibody fragment in the gastrointestinal tract could potentially be used. in order to develop such an alternative therapy, the effectiven ... | 2015 | 26092449 |
| relative cost-effectiveness of an extensively hydrolyzed casein formula containing the probiotic lactobacillus rhamnosus gg in managing infants with cow's milk allergy in italy. | to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (ehcf) containing the probiotic lactobacillus rhamnosus gg, (ehcf + lgg; nutramigen lgg) as first-line management for cow's milk allergy (cma) compared with ehcf alone, soy-based formulae (sbf), hydrolyzed rice formulae (hrf), and amino acid formulae (aaf) in italy, from the perspective of the italian national health service (inhs) and parents. | 2015 | 26089692 |
| lactobacillus rhamnosus gg supernatant upregulates serotonin transporter expression in intestinal epithelial cells and mice intestinal tissues. | the role that probiotics play in relieving irritable bowel syndrome (ibs) has been demonstrated; however, the mechanism by which ibs is affected remains unclear. in this study, serotonin transporter (sert) mrna and serotonin transporter protein (sert-p) levels in ht-29, caco-2 cells, and mice intestinal tissues were examined after treatment with lactobacillus rhamnosus gg supernatant (lgg-s). | 2015 | 26088715 |
| nasal priming with immunobiotic lactobacillus rhamnosus modulates inflammation-coagulation interactions and reduces influenza virus-associated pulmonary damage. | to evaluate the effect of the nasal administration of live and heat-killed lactobacillus rhamnosus crl1505 (lr1505) on immune-coagulative response during influenza virus (ifv) infection to improve survival and reduce lung injury. | 2015 | 26072063 |
| genome sequence of lactobacillus rhamnosus strain cncm i-3698. | lactobacillus rhamnosus cncm i-3698 is a commercially available probiotic that is used in animal feed as an additive. here, we announce the draft genome sequence for this strain, consisting of 71 contigs corresponding to 2,966,480 bp and a g+c content of 46.69%. | 2015 | 26067954 |
| lactobacillus rhamnosus bacteremia in a kidney transplant recipient. | lactobacillus rhamnosus is a rare clinical pathogen. a case of bacteremia caused by l. rhamnosus in a kidney transplant recipient is described. once considered only as a contaminant or a low-virulence organism, l. rhamnosus might be an opportunistic pathogen in immunocompromised patients. to our knowledge, this is the first report of primary bloodstream infection caused by l. rhamnosus in a kidney transplant recipient. | 2015 | 26061146 |
| lactobacillus rhamnosus gg improves glucose tolerance through alleviating er stress and suppressing macrophage activation in db/db mice. | although recent studies have reported that lactobacillus rhamnosus gg (lgg), the most extensively studied probiotic strain, exerts an anti-hyperglycemic effect on several rodent models, the underlying mechanism remains unclear. in this study, twenty male c57bl/ksj-db/db (db/db) mice were divided into 2 groups, lgg-treated and control group, which received a daily dose of lgg (1 × 10(8) cfu per mouse) and pbs orally for 4 weeks, respectively. we observed that glucose tolerance was significantly i ... | 2015 | 26060355 |
| immunomodulation and signaling mechanism of lactobacillus rhamnosus gg and its components on porcine intestinal epithelial cells stimulated by lipopolysaccharide. | this study aimed to evaluate the immunomodulatory effects and signaling mechanisms of lactobacillus rhamnosus gg (lgg) and its components [surface-layer protein (slp), dna, exopolysaccharides, and cpg oligodeoxynucleotides] on lipopolysaccharide (lps)-stimulated porcine intestinal epithelial cell (iec) ipec-j2. | 2015 | 26055689 |
| correlation of lactobacillus rhamnosus genotypes and carbohydrate utilization signatures determined by phenotype profiling. | lactobacillus rhamnosus is a bacterial species commonly colonizing the gastrointestinal (gi) tract of humans and also frequently used in food products. while some strains have been studied extensively, physiological variability among isolates of the species found in healthy humans or their diet is largely unexplored. the aim of this study was to characterize the diversity of carbohydrate utilization capabilities of human isolates and food-derived strains of l. rhamnosus in relation to their nich ... | 2015 | 26048937 |
| breakthrough lactobacillus rhamnosus gg bacteremia associated with probiotic use in an adult patient with severe active ulcerative colitis: case report and review of the literature. | probiotics are widely investigated in the treatment of various bowel diseases. however, they may also have a pathogenic potential, and the role of lactobacillus spp. as opportunistic pathogens, mostly following disruption of the intestinal mucosa, is emerging. | 2015 | 26024568 |
| effect of lactobacillus rhamnosus gg administration on vancomycin-resistant enterococcus colonization in adults with comorbidities. | vancomycin-resistant enterococci (vre) are endemic in health care settings. these organisms colonize the gastrointestinal tract and can lead to infection which is associated with increased mortality. there is no treatment for vre colonization. we conducted a randomized, double-blind, placebo-controlled clinical trial to examine the safety and efficacy of administration of the probiotic lactobacillus rhamnosus gg (lgg) for the reduction or elimination of intestinal colonization by vre. colonized ... | 2015 | 26014940 |
| sensory evaluation of locally-grown fruit purees and inulin fibre on probiotic yogurt in mwanza, tanzania and the microbial analysis of probiotic yogurt fortified with moringa oleifera. | the purpose of this study was to establish new food products that increase the nutritional value and health benefits of the probiotic yogurt currently used in the western heads east (whe) project in mwanza, tanzania. the probiotic yogurt has established health benefits, and product development through fortification must not adversely affect the acceptability of yogurt or the viability of the probiotics. both sensory testing and microbial analysis testing were conducted. the products tested were ... | 2015 | 25995722 |
| microbiota at multiple body sites during pregnancy in a rural tanzanian population and effects of moringa-supplemented probiotic yogurt. | the nutritional status of pregnant women is vital for healthy outcomes and is a concern for a large proportion of the world's population. the role of the microbiota in pregnancy and nutrition is a promising new area of study with potential health ramifications. in many african countries, maternal and infant death and morbidity are associated with malnutrition. here, we assess the influence of probiotic yogurt containing lactobacillus rhamnosus gr-1, supplemented with moringa plant as a source of ... | 2015 | 25979893 |
| draft genome sequence of lactobacillus rhamnosus cls17. | we announce the draft genome sequence of the type strain lactobacillus rhamnosus cls17 (2,889,314 nt, with a gc content of 46.8%), which is one of the most prevalent lactic acid bacteria present during the manufacturing process of dairy products; the genome consists of 71 large contigs (>100 bp in size). it contains 2,643 protein-coding sequences, single predicted copies of the 5s, 16s, and 23s rrna genes, and 51 predicted trnas. | 2015 | 25977410 |
| l-lactic acid production by lactobacillus rhamnosus atcc 10863. | lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid). l. rhamnosus atcc 10863 that produces l-lactic acid was used to perform the fermentation and molasses was used as substrate. a solution containing 27.6 g/l of sucrose (main composition of molasses) and 3.0 g/l of yeast extract was prepared, considering the final volume of 3,571 ml (14.0% (v/v) inoculum). batch and fed batch fermentations were performed with temperature of 43.4°c and ph of 5.0. ... | 2015 | 25922852 |
| functional dynamics of the gut microbiome in elderly people during probiotic consumption. | a mechanistic understanding of the purported health benefits conferred by consumption of probiotic bacteria has been limited by our knowledge of the resident gut microbiota and its interaction with the host. here, we detail the impact of a single-organism probiotic, lactobacillus rhamnosus gg atcc 53103 (lgg), on the structure and functional dynamics (gene expression) of the gut microbiota in a study of 12 healthy individuals, 65 to 80 years old. the analysis revealed that while the overall comm ... | 2015 | 25873374 |
| differences in dna methylation profile of th1 and th2 cytokine genes are associated with tolerance acquisition in children with ige-mediated cow's milk allergy. | epigenetic changes in dna methylation could regulate the expression of several allergy-related genes. we investigated whether tolerance acquisition in children with immunoglobulin e (ige)-mediated cow's milk allergy (cma) is characterized by a specific dna methylation profile of th2 (il-4, il-5) and th1 (il-10, ifn-γ)-associated cytokine genes. | 2015 | 25859290 |
| bugs for atopy: the lactobacillus rhamnosus gg strategy for food allergy prevention and treatment in children. | food allergy (fa) is a major health issue for children living in western countries. at this time the only proven treatment for fa is elimination of offender antigen from the diet. it is becoming clear that the development of gut microbiota exerts a profound influence on immune system maturation and tolerance acquisition. increasing evidence suggests that perturbations in gut microbiota composition of infants are implicated in the pathogenesis of fa. these findings have unveiled new strategies to ... | 2015 | 25851101 |
| saccharomyces cerevisiae ec-1118 enhances the survivability of probiotic lactobacillus rhamnosus hn001 in an acidic environment. | the present study attempted to partially characterize and elucidate the viability-enhancing effect of a yeast strain saccharomyces cerevisiae ec-1118 on a probiotic strain lactobacillus rhamnosus hn001 under acidic conditions using a model system (non-growing cells). the yeast was found to significantly enhance (p < 0.05) the viability of the probiotic strain under acidic conditions (ph 2.5 to 4.0) by 2 to 4 log cycles, and the viability-enhancing effects were observed to be influenced by ph, an ... | 2015 | 25846337 |
| beneficial bacteria affect danio rerio development by the modulation of maternal factors involved in autophagic, apoptotic and dorsalizing processes. | probiotic strains have been recognized to exert important roles in many biological systems, including immune response, growth, development and reproduction. however, to date, no studies have focused either on the relation among probiotics and maternal factors or on probiotics' ability to qualitatively and/or quantitatively modulate maternal transcripts. | 2015 | 25833742 |
| expression of the toxin-antitoxin genes yefm(lrh), yoeb(lrh) in human lactobacillus rhamnosus isolates. | lactobacilli are important microorganisms in various activities, for example, diary products, meat ripening, bread and pickles, but, moreover, are associated directly with human skin and cavities (e.g., mouth, gut, or vagina). some of them are used as probiotics. therefore, the molecular biological investigation of these bacteria is important. earlier we described several toxin antitoxin systems (type ii) in lactobacilli. here, we describe the structure and transcriptional regulation of genes, e ... | 2015 | 25832734 |
| lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism. | the microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. analysis of bacterial 16s libraries revealed that lactobacillus rhamnosus was able to modulate the gut microbi ... | 2015 | 25822072 |
| human dendritic cell dc-sign and tlr-2 mediate complementary immune regulatory activities in response to lactobacillus rhamnosus jb-1. | the microbiota is required for optimal host development and ongoing immune homeostasis. lactobacilli are common inhabitants of the mammalian large intestine and immunoregulatory effects have been described for certain, but not all, strains. the mechanisms underpinning these protective effects are beginning to be elucidated. one such protective organism is lactobacillus rhamnosus jb-1 (lb. rhamnosus jb-1). lb. murinus has no such anti-inflammatory protective effects and was used as a comparator o ... | 2015 | 25816321 |
| lactobacillus rhamnosus gg in adenoid tissue: double-blind, placebo-controlled, randomized clinical trial. | lactobacillus rhamnosus gg (l.gg) was present in all adenoids of children receiving the l. gg product. however, since l.gg was also found from the placebo group, one cannot confirm its effect on the occurrence of rhinovirus (rv) or enterovirus (ev). | 2015 | 25813240 |
| cost-effectiveness of using an extensively hydrolyzed casein formula plus the probiotic lactobacillus rhamnosus gg compared to an extensively hydrolyzed formula alone or an amino acid formula as first-line dietary management for cow's milk allergy in the us. | the aim was to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (ehcf) plus the probiotic lactobacillus rhamnosus gg (ehcf + lgg; nutramigen lgg) compared to an ehcf alone (nutramigen) and an amino acid formula (aaf; neocate) as first-line dietary management for cow's milk allergy (cma) in the us. | 2015 | 25767400 |
| a possible link between early probiotic intervention and the risk of neuropsychiatric disorders later in childhood: a randomized trial. | recent experimental evidence suggests that gut microbiota may alter function within the nervous system providing new insight on the mechanism of neuropsychiatric disorders. | 2015 | 25760553 |
| inhibition of mir122a by lactobacillus rhamnosus gg culture supernatant increases intestinal occludin expression and protects mice from alcoholic liver disease. | alcoholic liver disease (ald) has a high morbidity and mortality. chronic alcohol consumption causes disruption of intestinal microflora homeostasis, intestinal tight junction barrier dysfunction, increased endotoxemia, and eventually liver steatosis/steatohepatitis. probiotic lactobacillus rhamnosus gg (lgg) and the bacteria-free lgg culture supernatant (lggs) have been shown to promote intestinal epithelial integrity and protect intestinal barrier function in ald. however, little is known abou ... | 2015 | 25746479 |
| non-viable immunobiotic lactobacillus rhamnosus crl1505 and its peptidoglycan improve systemic and respiratory innate immune response during recovery of immunocompromised-malnourished mice. | the effect of non-viable lactobacillus rhamnosus crl1505 and its cell wall and peptidoglycan on respiratory immunity in malnourished mice was studied. weaned mice were malnourished with a protein-free diet for 21d and received bcd during 7d (bcd) or bcd with nasal non-viable l. rhamnosus crl1505 (bcd+uv) or its cell wall (bcd+cw) or peptidoglycan (bcd+pg) supplementation during last 2d of the treatment. malnourished mice without treatment (mnc) and well-nourished mice (wnc) were used as controls ... | 2015 | 25744605 |
| probiotics (lactobacillus rhamnosus r0011 and acidophilus r0052) reduce the expression of toll-like receptor 4 in mice with alcoholic liver disease. | the role of lipopolysaccharide (lps) and toll-like receptor 4 (tlr 4) in the pathogenesis of alcoholic liver disease (ald) has been widely established. we evaluated the biological effects of probiotics (lactobacillus rhamnosus r0011 and acidophilus r0052), krg (korea red ginseng), and urushiol (rhus verniciflua stokes) on ald, including their effects on normal and high-fat diet in mice. | 2015 | 25692549 |
| nonpharmacologic treatment of functional abdominal pain disorders: a systematic review. | various nonpharmacologic treatments are available for pediatric abdominal pain-related functional gastrointestinal disorders (ap-fgids). data on efficacy and safety are scarce. the goal of this study was to summarize the evidence regarding nonpharmacologic interventions for pediatric ap-fgids: lifestyle interventions, dietary interventions, behavioral interventions, prebiotics and probiotics, and alternative medicine. | 2015 | 25667239 |
| [irritable bowel syndrome; gut microbiota and probiotic therapy]. | irritable bowel syndrome (ibs) is characterized by symptoms of abdominal pain and altered bowel habits. this common disorder is managed by varying clinical styles as no dominant therapeutic strategy has emerged. the pathophysiology of ibs remains unknown, but several lines of evidence link this disorder with the gut microbiota. although controversy exists, gut microbiota is likely contributing to symptoms of ibs, at least in some patients, through an altered fermentation process, an impaired int ... | 2015 | 25659060 |
| [probiotics for the treating acute diarrhea and preventing antibiotic-associated diarrhea in children]. | probiotics are helpful in the treatment of acute diarrhea. several systematic reviews show that the use of probiotics shortens the duration of diarrhea in one day and reduces by 59% the risk of diarrhea lasting longer than 4 days. hospital stay is 24 hours shorter in children treated with probiotics. the benefitial effect of probiotics is species-specific; lactobacillus rhamnosus gg and saccharomyces boulardii are the two species with proven efficacy in the treatment of acute diarrhea. the use o ... | 2015 | 25659056 |
| fermented red ginseng extract inhibits cancer cell proliferation and viability. | red ginseng (panax ginseng c.a. meyer) is the most widely recognized medicinal herb due to its remedial effects in various disorders, such as cancers, diabetes, and heart problems. in this study, we investigated the anticancer effect of fermented red ginseng extract (f-rge; provided by jeonju biomaterials institute, jeonju, south korea) in a parallel comparison with the effect of nonfermented red ginseng extract (nf-rge; control) on several cancer cell lines--mcf-7 breast cancer cells, hepg2 hep ... | 2015 | 25658580 |
| influence of orally administered lactobacillus gg on respiratory immune response in a murine model of diet-induced obesity. | mice with diet-induced obesity were fed with lactobacillus rhamnosus gg (lgg) suspended in saline or saline alone (control mice). pulmonary mrna expression of ifn-γ; ifn-α receptor 1; cd247 antigen; killer cell lectin-like receptor subfamily k, member 1; tnf-α; il-12 receptor β1 and il-2 receptor β, and the proportion of lactobacillales in feces were significantly greater in the lgg group than in the control mice (p < 0.05 and p < 0.01, respectively). these results suggest that lgg alters the re ... | 2015 | 25643737 |
| modulatory activity of lactobacillus rhamnosus oll2838 in a mouse model of intestinal immunopathology. | gut microbiota and probiotic strains play an important role in oral tolerance by modulating regulatory and effector cell components of the immune system. we have previously described the ability of lactobacilli to influence both the innate and adaptive immunity to wheat gluten, a food antigen, in mouse. in this study, we further explored the immunomodulatory mechanisms elicited in this model by testing three specific probiotic strains, namely l. rhamnosus oll2838, b. infantis atcc15697 and s. th ... | 2015 | 25623030 |
| enhanced ampk phosphorylation contributes to the beneficial effects of lactobacillus rhamnosus gg supernatant on chronic-alcohol-induced fatty liver disease. | we have previously demonstrated that lactobacillus rhamnosus gg culture supernatant (lggs) prevents acute-alcohol-exposure-induced hepatic steatosis and injury. the protective effects of lggs were attributed to the improved intestinal barrier function leading to decreased endotoxemia. the purpose of this study was to determine whether lggs was effective in protecting against chronic-alcohol-induced hepatic steatosis and injury and to evaluate the underlying mechanisms of lggs on hepatic lipid me ... | 2015 | 25622859 |
| administration of a probiotic with peanut oral immunotherapy: a randomized trial. | coadministration of a bacterial adjuvant with oral immunotherapy (oit) has been suggested as a potential treatment for food allergy. | 2015 | 25592987 |
| hepatic and fecal metabolomic analysis of the effects of lactobacillus rhamnosus gg on alcoholic fatty liver disease in mice. | the interactions among the gut, liver, and immune system play an important role in liver disease. probiotics have been used for the treatment and prevention of many pathological conditions, including liver diseases. comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (gc×gc-tof ms) was used herein, in conjunction with chemometric data analysis, to identify metabolites significantly affected by probiotics in mice fed with or without alcohol. the metabolomics analysis ... | 2015 | 25592873 |
| lactobacillus rhamnosus gg and its spac pilus adhesin modulate inflammatory responsiveness and tlr-related gene expression in the fetal human gut. | bacterial contact in utero modulates fetal and neonatal immune responses. maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. we investigated the immunomodulatory properties of live lactobacillus rhamnosus gg and its spac pilus adhesin in human fetal intestinal models. | 2015 | 25580735 |
| piliation of lactobacillus rhamnosus gg promotes adhesion, phagocytosis, and cytokine modulation in macrophages. | recently, spacba-encoded pili on the cell surface of lactobacillus rhamnosus gg were identified to be key molecules for binding to human intestinal mucus and caco-2 intestinal epithelial cells. here, we investigated the role of the spacba pilus of l. rhamnosus gg in the interaction with macrophages in vitro by comparing the wild type with surface mutants. our results show that spacba pili play a significant role in the capacity for adhesion to macrophages and also promote bacterial uptake by the ... | 2015 | 25576613 |
| lactobacillus rhamnosus gg dosage affects the adjuvanticity and protection against rotavirus diarrhea in gnotobiotic pigs. | the use of immunostimulatory strains of probiotics as adjuvants has been increasingly recognized as a promising approach in enhancing vaccine immunogenicity; however, dose effects of probiotic adjuvants are not well defined. in the present study, we examined dose effects of a commonly used probiotic strain, lactobacillus rhamnosus gg (lgg), on immunomodulation with 2 different dosages. | 2015 | 25564808 |
| microencapsulation of alginate-immobilized bagasse with lactobacillus rhamnosus nrrl 442: enhancement of survivability and thermotolerance. | the aim of this research was to enhance the survivability of lactobacillus rhamnosus nrrl 442 against heat exposure via a combination of immobilization and microencapsulation processes using sugarcane bagasse (sb) and sodium alginate (naa), respectively. the microcapsules were synthesized using different alginate concentration of 1, 2 and 3% and naa:sb ratio of 1:0, 1:1 and 1:1.5. this beneficial step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation ... | 2015 | 25563958 |
| production of lactic acid from the mixture of softwood pre-hydrolysate and paper mill sludge by simultaneous saccharification and fermentation. | paper mill sludge is a solid waste material composed of pulp residues and ash generated from pulping and paper making process. the carbohydrate portion of the sludges from kraft/recycle paper mill has chemical and physical characteristics similar to those of commercial wood pulp. because of its high carbohydrate content and well-dispersed structure, the sludge can be biologically converted to value-added products without pretreatment. in bioconversion of solid feedstock such as paper mill sludge ... | 2015 | 25561054 |
| [susceptibility of lactobacillus rhamnosus strains isolated from probiotic products to antimicrobial agents]. | probiotics generally recognized as safe (gras), when given in relevant dose, are able to induce strain-specific beneficial effects for health of humans or animals. | 2015 | 27019914 |
| uncovering surface-exposed antigens of lactobacillus rhamnosus by cell shaving proteomics and two-dimensional immunoblotting. | the present study reports the identification and comparison of all expressed cell-surface exposed proteins from the well-known probiotic l. rhamnosus gg and a related dairy strain, lc705. to obtain this information, the cell-surface bound proteins were released from intact cells by trypsin shaving under hypertonic conditions with and without dtt. liquid chromatography tandem mass spectrometry (lc-ms/ms) analyses of the purified peptides identified a total of 102 and 198 individual proteins from ... | 2015 | 25531588 |
| physicochemical characterization of exopolysaccharides produced by lactobacillus rhamnosus on various carbon sources. | the impact of five carbohydrate sources (glucose, maltose, galactose, sucrose, and lactose) on the chemical composition, structure, morphology, and physicochemical properties, as well as, viscosity of exopolysaccharides (epss) produced by lactobacillus rhamnosus e/n was investigated. glc-ms analysis and 2dnmr spectroscopy showed that the epss had the same primary structure independently of the carbon source used in the growth medium. the following eps composition was elucidated: four rhamnose, t ... | 2015 | 25498664 |
| characterization of the antifungal activity of lactobacillus harbinensis k.v9.3.1np and lactobacillus rhamnosus k.c8.3.1i in yogurt. | few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. consumer's demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. in this study, using a 2(5-1) factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contaminati ... | 2015 | 25481057 |
| the use of flow cytometry to accurately ascertain total and viable counts of lactobacillus rhamnosus in chocolate. | the goals of this study were to evaluate the precision and accuracy of flow cytometry (fc) methodologies in the evaluation of populations of probiotic bacteria (lactobacillus rhamnosus r0011) in two commercial dried forms, and ascertain the challenges in enumerating them in a chocolate matrix. fc analyses of total (fc(t)) and viable (fc(v)) counts in liquid or dried cultures were almost two times more precise (reproducible) than traditional direct microscopic counts (dcm) or colony forming units ... | 2015 | 25475282 |
| ehealth: low fodmap diet vs lactobacillus rhamnosus gg in irritable bowel syndrome. | to investigate the effects of a low fermentable, oligosaccharides, disaccharides, monosaccharides and polyols diet (lfd) and the probiotic lactobacillus rhamnosus gg (lgg) in irritable bowel syndrome (ibs). | 2014 | 25473176 |
| probiotics: an update. | triggered by the growing knowledge on the link between the intestinal microbiome and human health, the interest in probiotics is ever increasing. the authors aimed to review the recent literature on probiotics, from definitions to clinical benefits, with emphasis on children. | 2015 | 25458874 |
| molecular characteristics of an exopolysaccharide from lactobacillus rhamnosus kf5 in solution. | the molecular weight and chain conformation of an exopolysaccharide (eps), s2, from lactobacillus rhamnosus kf5 were determined by size-exclusion chromatography combined with multi-angle laser light scattering (sec-malls), dynamic light scattering (dls), viscometry and atomic force microscopy (afm). the weight-average molecular weight (mw), intrinsic viscosity [ƞ], radii of gyration (r(g)) and hydrodynamic radii (r(h)) were 7.5 × 10(5) da, 2.331 dl/g, 44.3 nm and 29.4 nm, respectively. the confo ... | 2015 | 25453290 |
| effects of immunomodulatory supplementation with lactobacillus rhamnosus on airway inflammation in a mouse asthma model. | asthma is a common allergic disease. in previous studies, probiotics improved the balance of intestinal microbes, reduced inflammation, and promoted mucosal tolerance. this study investigated whether oral administrations of lactobacillus rhamnosus gg (lgg) inhibited allergen (ovalbumin or ova)-induced airway inflammation in a mouse asthma model. | 2016 | 25440975 |
| no evidence of harms of probiotic lactobacillus rhamnosus gg atcc 53103 in healthy elderly-a phase i open label study to assess safety, tolerability and cytokine responses. | although lactobacillus rhamnosus gg atcc 53103 (lgg) has been consumed by 2 to 5 million people daily since the mid 1990s, there are few clinical trials describing potential harms of lgg, particularly in the elderly. | 2014 | 25438151 |
| lactobacillus rhamnosus gg reverses insulin resistance but does not block its onset in diet-induced obese mice. | recently, lactobacillus rhamnosus gg (lgg) was shown to exert insulin-sensitizing and adiposity-reducing effects in high-fat (hf) diet-fed mice. in the present study, we observed that the effects were correlated with the extent of dysbiosis induced by hf diet feeding before lgg administration. lgg-treated mice were protected from hf diet-induced adiposity and/ or insulin resistance when lgg was treated after, not along with, hf diet feeding. results indicate that, under hf dietary condition, sup ... | 2015 | 25433553 |
| brain and liver fatty acid composition changes upon consumption of lactobacillus rhamnosus la68. | recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. there are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. this study was designed to test the effects of a particular lactobacillus strain, lactobacillus rhamnosus la68 on fatty acid composition of the liver and the brain of c57bl/6 mice in the absence of an underlying pat ... | 2015 | 25431181 |
| efficacy of corn silage inoculants on the fermentation quality under farm conditions and their influence on aspergillus parasitucus, a. flavus and a. fumigatus determined by q-pcr. | laboratory-scale silos were prepared to evaluate the efficacy of two different lactic acid bacteria (lab) on the fermentation quality and mycobiota of corn silage. their influence on aspergillus species' variability by using the q-pcr technique was studied. silage inoculated with lactobacillus rhamnosus rc007 or l. plantarum rc009 were compared with uninoculated silage. silos were opened after 1, 7, 45, 90 and 120 days after ensiling. at the end of the ensiling period, silos were left open for 7 ... | 2015 | 25421370 |
| effect of lactobacillus rhamnosus hsryfm 1301 on the gut microbiota and lipid metabolism in rats fed a high-fat diet. | accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. to investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed using real-time pcr before and after administration of lactobacillus rhamnosus hsryfm 1301 and its fermented milk for 28 days. the findings showed that the lactobacillus spp., bifi ... | 2015 | 25418480 |
| phenotypical analysis of the lactobacillus rhamnosus gg fimbrial spafed operon: surface expression and functional characterization of recombinant spafed pili in lactococcus lactis. | a noticeable genomic feature of many piliated gram-positive bacterial species is the presence of more than one pilus-encoding operon. paradigmatically, the gut-adapted lactobacillus rhamnosus gg strain contains two different fimbrial operons in its genome. however, whereas one of these operons (called spacba) is encoding for the functionally mucus-/collagen-binding spacba pilus, for the other operon (called spafed) any native expression of the spafed-called pili is still the subject of some unce ... | 2014 | 25415357 |
| reduced neonatal regulatory t cell response to microbial stimuli associates with subsequent eczema in high-risk infants. | regulatory t cells (treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. we evaluated cord blood treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year. | 2014 | 25376403 |
| survival of pathogenic and lactobacilli species of fermented olives during simulated human digestion. | the present survey uses a dynamic gastric and small intestinal model to assess the survival of one pathogenic (escherichia coli o157:h7 edl 933) and three lactobacilli bacteria with probiotic potential (lactobacillus rhamnosus gg, l. pentosus tomc-lab2, and l. pentosus tomc-lab4) during their passage through the human gastrointestinal tract using fermented olives as the food matrix. the data showed that the survival of the e. coli strain in the stomach and duodenum was very low, while its transi ... | 2014 | 25352842 |
| adhesion properties of lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins. | we previously described potential probiotic lactobacillus rhamnosus strains, isolated from fermented mare milk produced in sumbawa island, indonesia, which showed high adhesion to porcine colonic mucin (pcm) and extracellular matrix (ecm) proteins. recently, mucus-binding factor (mbf) was found in the gg strain of l. rhamnosus as a mucin-binding protein. in this study, we assessed the ability of recombinant mbf protein from the fsmm22 strain, one of the isolates of l. rhamnosus from fermented su ... | 2015 | 25351253 |
| probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs. | we generated a neonatal pig model with human infant gut microbiota (hgm) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. all the hgm-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. the gut microbiota of vaccinated pigs were investigated for effects of lactobacillus rhamnosus gg (lgg) supplement and homotypic virulent human rotavirus (hrv) challenge. high-throughput sequencing of v4 r ... | 2014 | 25349634 |
| biosorption of al(+3) and cd(+2) by an exopolysaccharide from lactobacillus rhamnosus. | the aim of this study was to assess the removal of cd(2+) and al(3+) from aqueous solutions by an exopolysaccharide (eps) from lactobacillus rhamnosus e/n. the biosorption kinetics of eps for cd(2+) and al(3+) were studied by equilibrium dialysis after incubation from 0 h to 58 h. the actual concentrations of cd(2+) and al(3+) in external solutions were measured by a fluorescence method after complexation with 8-hydroxyquinoline. binding of metal ions by eps was observed, with maximum adsorption ... | 2014 | 25308465 |
| oral intake of lactobacillus rhamnosus m21 enhances the survival rate of mice lethally infected with influenza virus. | influenza viruses cause acute respiratory disease. because of the high genetic variability of viruses, effective vaccines and antiviral agents are limited. considering the fact that the site of influenza virus entry is the mucosa of the upper respiratory tract, probiotics that can enhance mucosal immunity as well as systemic immunity could be an important source of treatment against influenza infection. | 2016 | 25304268 |
| the effect of lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model. | growing evidence indicates that intestinal microbiota regulate our metabolism. probiotics confer health benefits that may depend on their ability to affect the gut microbiota. the objective of this study was to examine the effect of supplementation with the probiotic strain, lactobacillus rhamnosus hsryfm 1301, on the gut microbiota in a hyperlipidemic rat model, and to explore the associations between the gut microbiota and the serum lipids. | 2014 | 25300818 |
| probiotic lactobacillus rhamnosus gg (lgg) and prebiotic prevent neonatal inflammation-induced visceral hypersensitivity in adult rats. | increasing evidence indicates a positive effect of probiotics on the nervous system. the objective of this study was to determine if probiotic lactobacillus rhamnosus gg (lgg) and/or prebiotics polydextrose/galactooligosaccharide (pdx/gos) can alter the colonic sensitivity in a neonatal rat model of chronic visceral hyperalgesia and to determine whether altered sensitivity is associated with changes in neurotransmitter levels in the brain. | 2014 | 25298006 |
| randomized open-label pilot study of the influence of probiotics and the gut microbiome on toxic metal levels in tanzanian pregnant women and school children. | exposure to environmental toxins is a 21st century global health problem that is often the result of dietary intake. although efforts are made to reduce dietary toxin levels, they are often unsuccessful, warranting research into novel methods to reduce host exposure. food-grade microbes that can be delivered to the gastrointestinal tract and that are capable of sequestering toxins present a safe and cost-effective intervention. we sought to investigate the potential for probiotic-supplemented yo ... | 2014 | 25293764 |
| influence of manufacturing processes on cell surface properties of probiotic strain lactobacillus rhamnosus lcr35®. | the influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. in the present study, we comparatively assessed the cell wall characteristics of the probiotic strain lactobacillus rhamnosus lcr35® together with three of its commercial formulations with intestinal applications. putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of l. rhamnosus lcr35®. a tot ... | 2015 | 25280746 |
| tolerability of a probiotic in subjects with a history of methicillin-resistant staphylococcus aureus colonisation. | methicillin-resistant staphylococcus aureus (mrsa) is a pathogen of major public health importance. colonisation precedes infection; thus reducing mrsa carriage may be of benefit for reducing infection. probiotics represent a novel approach to reducing mrsa carriage. we undertook a pilot feasibility randomised controlled trial of the tolerability and acceptability of probiotics for reducing nasal and intestinal carriage of mrsa. in addition, subjects were screened for vancomycin-resistant entero ... | 2014 | 25213147 |
| endogenous biotin-binding proteins: an overlooked factor causing false positives in streptavidin-based protein detection. | biotinylation is widely used in dna, rna and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. during the streptavidin-based detection of glycoproteins in lactobacillus rhamnosus gg with biotinylated lectin probes, a strong positive band of approximately 125 kda was observed, present in different cellular fractions. this potential glycoprotein reacted heavily with concanavalin a (cona), a lectin that specifically binds glucose and mannos ... | 2015 | 25211245 |
| draft genome sequences and description of lactobacillus rhamnosus strains l31, l34, and l35. | lactobacillus rhamnosus is a facultative, lactic acid bacterium in the phylum firmicutes. lactobacillus spp. are generally considered beneficial, and specific strains of l. rhamnosus are validated probiotics. we describe the draft genomes of three l. rhamnosus strains (l31, l34, and l35) isolated from the feces of thai breastfed infants, which exhibit anti-inflammatory properties in vitro. the three genomes range between 2.8 - 2.9 mb, and contain approximately 2,700 protein coding genes. | 2014 | 25197459 |
| genomic characterization of non-mucus-adherent derivatives of lactobacillus rhamnosus gg reveals genes affecting pilus biogenesis. | lactobacillus rhamnosus gg is one of the best-characterized lactic acid bacteria and can be considered a probiotic paradigm. comparative and functional genome analysis showed that l. rhamnosus gg harbors a genomic island including the spacba-srtc1 gene cluster, encoding the cell surface-decorating host-interacting pili. here, induced mutagenesis was used to study pilus biogenesis in l. rhamnosus gg. a combination of two powerful approaches, mutation selection and next-generation sequencing, was ... | 2014 | 25192985 |
| towards a better understanding of lactobacillus rhamnosus gg--host interactions. | lactobacillus rhamnosus gg (lgg) is one of the most widely used probiotic strains. various health effects are well documented including the prevention and treatment of gastro-intestinal infections and diarrhea, and stimulation of immune responses that promote vaccination or even prevent certain allergic symptoms. however, not all intervention studies could show a clinical benefit and even for the same conditions, the results are not univocal. clearly, the host phenotype governed by age, genetics ... | 2014 | 25186587 |
| study of the lactobacillus rhamnosus lcr35® properties after compression and proposition of a model to predict tablet stability. | the beneficial effects of probiotic bacteria on human health are now widely acknowledged, and this has prompted growing interest in research and development in the pharmaceutical field. however, to be viable when they reach their target, the bacteria must be able to survive during the manufacturing process and the biological pathway. tablet form best meets the requirements for protecting acid labile drugs, but the tableting process could be an additional stress for the bacteria. this study evalu ... | 2014 | 25128853 |
| severe oral infection due to lactobacillus rhamnosus during induction chemotherapy for acute myeloid leukemia. | we report a case of severe oral infection with a high fever due to lactobacillus rhamnosus during induction chemotherapy for acute myeloid leukemia. the patient did not improve on treatment with meropenem, clindamycin, or vancomycin until neutrophil recovery. since l. rhamnosus gg is used in dairy products, and the patient ingested dairy products daily before starting chemotherapy, we suspected an association between the ingestion of dairy products and the development of infection. pulsed-field ... | 2014 | 25115834 |
| optimization of culture conditions for exopolysaccharide production by a probiotic strain of lactobacillus rhamnosus e/n. | the effects of culture conditions on exopolysaccharides (eps) production by a probiotic lb. rhamnosus e/n have been studied using the plackett-burman design. process optimization was performed in stationary cultures to maximize the production of eps. in order to verify the optimal conditions, an analysis was performed of eps production in fermentation culture. batch fermentation was carried out at working volume of 2.51. the optimal temperature, ph, carbon source, and nitrogen source conditions ... | 2014 | 25115122 |
| the effect of cell surface components on adhesion ability of lactobacillus rhamnosus. | the aim of this study was to analyze the cell envelope components and surface properties of two phenotypes of lactobacillus rhamnosus isolated from the human gastrointestinal tract. the ability of the bacteria to adhere to human intestinal cells and to aggregate with other bacteria was determined. l. rhamnosus strains e/n and pen differed with regard to the presence of exopolysaccharides (eps) and specific surface proteins. transmission electron microscopy showed differences in the structure of ... | 2014 | 25090959 |
| probiotic edible films as a new strategy for developing functional bakery products: the case of pan bread. | in the present paper, a novel approach for the development of probiotic baked cereal products is presented. probiotic pan bread constructed by the application of film forming solutions based either on individual hydrogels e.g. 1% w/w sodium alginate (alg) or binary blends of 0.5% w/w sodium alginate and 2% whey protein concentrate (alg/wpc) containing lactobacillus rhamnosus gg, followed by an air drying step at 60 °c for 10 min or 180 °c for min were produced. no visual differences between the ... | 2014 | 25089068 |
| lactobacillus rhamnosus gg in the middle ear after randomized, double-blind, placebo-controlled oral administration. | probiotics may have potency in reducing upper respiratory infections, in particular in children. we studied findings from middle ear effusion (mee) samples after randomized, placebo-controlled 3-week oral administration of probiotic lactobacillus rhamnosus gg (l. | 2014 | 25085073 |
| preliminary evaluation of probiotic properties of lactobacillus strains isolated from sardinian dairy products. | twenty-three lactobacillus strains of dairy origin were evaluated for some functional properties relevant to their use as probiotics. a preliminary subtractive screening based on the abilities to inhibit the growth of microbial pathogens and hydrolyze conjugated bile salts was applied, and six strains were selected for further characterization including survival under gastrointestinal environmental conditions, adhesion to gut epithelial tissue, enzymatic activity, and some safety properties. all ... | 2014 | 25054135 |