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[probiotic monotherapy of bacterial vaginosis: a open, randomized trial].the aim of the current research is to establish the clinical and microbiological efficacy of the therapy with local probiotics in the treatment of anaerobic vaginal infections and the therapy's effect upon vaginal flora.201324294744
influence of fructooligosaccharides on the fermentation profile and viable counts in a symbiotic low fat milk.this study evaluated the effects of prebiotics on fermentation profile and growth of lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus rhamnosus, and bifidobacterium lactis in co-cultures with streptococcus thermophilus. acidification rate and viability were positively influenced by the co-culture with b. lactis and by both inulin or oligofructose in low fat milk.201324294233
[efficacy of combined 5-nitroimidazole and probiotic therapy of bacterial vaginosis: randomized open trial].the aim of the current research is to identify the clinical and microbiological effect of 5-nitroimidazol therapy for the treatment of bacterial vaginosis and in combination with probiotics and the influence of such therapy upon vaginal flora.201324283058
in vitro fermentation of prebiotic oligosaccharides by bifidobacterium lactis hn019 and lactobacillus spp.the utilisation of various prebiotic oligosaccharides by probiotic strains of bifidobacterium lactis, lactobacillus rhamnosus and lactobacillus acidophilus was investigated in order to determine the synbiotic potential of various prebiotic/probiotic combinations. analysis by hplc and high-performance anion-exchange chromatography of the cell-free medium taken during growth of the three probiotic bacteria showed differences in the consumption of the various oligosaccharides. analysis of galactool ...201424239979
a double-blind placebo-controlled randomized trial on probiotics in small bowel bacterial overgrowth in children treated with omeprazole.to evaluate the incidence of small bowel bacterial overgrowth (sbbo) in children treated with omeprazole, and to test whether probiotics influence the incidence.201323796359
effects of three-month intake of synbiotic on inflammation and body composition in the elderly: a pilot study.we hypothesize that improvements in the gut microbiota are capable of ameliorating gut permeability and, consequently, reducing systemic inflammation and the risk of frailty. this study aims to evaluate some effects of synbiotic supplementation on inflammatory markers and the body composition of the elderly at risk of frailty. in a double-blind study that lasted three months, 17 elderly individuals fulfilling one frailty criteria (grip strength) were randomly distributed into two groups: syn (n ...201323595135
alleviating effects of lactobacillus strains on pathogenic vibrio parahaemolyticus-induced intestinal fluid accumulation in the mouse model.the aim of this study was to evaluate the probiotic effects of lactobacillus strains against vibrio parahaemolyticus causing gastroenteritis. six-week-old icr mice were pretreated with four lactobacillus strains at three dosages, and then challenged with v. parahaemolyticus tgqx01 (serotype o3:k6). the results showed that v. parahaemolyticus tgqx01 caused severe intestinal fluid accumulation (fa) and villi damage in control mice which were pretreated with phosphate-buffered saline. in contrast, ...201323210909
[assessment of antagonistic activity in vitro lactobacillus spp. strains againts clostridum difficile strains isolated from gastrointestinal tract of patients hospitalized in three hospitals in region mazovia].the aim of this study was to evaluate the antagonistic activity of lactobacillus strains against clinical c. difficile strains isolated from faecal samples of adults patients with diarrhea. a total 61 strains of c. difficile randomly selected isolated in the period 2007-2008 from the gastrointestinal tract of hospitalized patients in three hospitals province mazovia, poland. to determination of antagonistic activity ofprobiotic lactobacillus spp. strains used four reference strains: lactobacillu ...201223072055
lactobacillus plantarum cs24.2 prevents escherichia coli adhesion to ht-29 cells and also down-regulates enteropathogen-induced tumor necrosis factor-α and interleukin-8 expression.the aim of the present study was to evaluate the potential of lactobacillus plantarum cs24.2 to antagonize escherichia coli adhesion and modulate expression of the responses by ht-29 cells of inflammatory molecules to e. coli adhesion. experiments were performed under different adhesion conditions and findings compared with the responses of lactobacillus rhamnosus gg. tests of competitive adhesion, adhesion inhibition and displacement assays were performed for lactobacilli (l. rhamnosus gg and l ...201323586634
study of probiotic potential of four wild lactobacillus rhamnosus strains.the four wild lactobacillus rhamnosus strains were examined in vitro for resistance to simulated gastro and intestinal juices, adhesion to ht-29 cells, antagonistic activity against enteric pathogens and immunomodulating activity. the strains l. rhamnosus sb5l, j5l and in1l were able to survive in simulated gastro juice while the strain l. rhamnosus sb31l lost viability exposed to simulated gastro juice for 3 h. the four strains had high viability in simulated small intestinal juice with little ...201323528983
non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium.the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ...201526280244
comparison of the kinetics of intestinal colonization by associating 5 probiotic bacteria assumed either in a microencapsulated or in a traditional, uncoated form.beneficial findings concerning probiotics are increasing day by day. however, one of the most important parameters able to significantly affect the probiotic value of a microorganism is its survival during the transit through the stomach and the duodenum. some techniques may be applied that aim to improve this parameter, but microencapsulation of bacterial cells remains one of the most important. a recent study assessed the kinetics of intestinal colonization by a mixture of 2 probiotic strains, ...201222955366
cost/benefit of synbiotics in acute infectious gastroenteritis: spend to save.the cost/benefit ratio of probiotics in the ambulatory treatment of acute infectious gastro-enteritis with or without a synbiotic food supplement (containing fructo-oligosaccharides and probiotic strains of streptoccoccus thermophilus, lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacterium lactis and bifidobacterium infantis) has been studied. 111 children (median age 37 and 43 months for the synbiotic and placebo group, respectively) with acute infectious gastroenteritis were inclu ...201222835702
evaluation of lactobacillus rhamnosus gg and lactobacillus acidophilus ncfm encapsulated using a novel impinging aerosol method in fruit food products.this study investigated the effect of microencapsulation on the survival of lactobacillus rhamnosus gg and lactobacillus acidophilus ncfm and their acidification in orange juice at 25°c for nine days and at 4°c over thirty five days of storage. alginate micro beads (10-40 μm) containing the probiotics were produced by a novel dual aerosol method of alginate and cacl(2) cross linking solution. unencapsulated l. rhamnosus gg was found to have excellent survivability in orange juice at both tempera ...201222633536
double-blind, randomised clinical assay to evaluate the efficacy of probiotics in preterm newborns weighing less than 1500 g in the prevention of necrotising enterocolitis.a randomised, double-blind clinical trial was undertaken in order to assess the effectiveness of probiotics in the prevention of necrotising enterocolitis (nec) in newborns weighing <1500 g.201322556209
detection and specific enumeration of multi-strain probiotics in the lumen contents and mucus layers of the rat intestine after oral administration.although the detection of viable probiotic bacteria following their ingestion and passage through the gastrointestinal tract (git) has been well documented, their mucosal attachment in vivo is more difficult to assess. in this study, we investigated the survival and mucosal attachment of multi-strain probiotics transiting the rat git. rats were administered a commercial mixture of the intestinal probiotics lactobacillus acidophilus la742, lactobacillus rhamnosus l2h and bifidobacterium lactis hn ...200926783165
identification of lactobacillus strains of goose origin using maldi-tof mass spectrometry and 16s-23s rdna intergenic spacer pcr analysis.the objective of our study was to identify lactobacillus sp. strains of goose origin using maldi-tof mass spectrometry, its-pcr and its-pcr/rflp. all three techniques proved to be valuable tools for identification of avian lactobacilli and produced comparable classification results. lactobacillus strains were isolated from 100% of geese aged 3 weeks to 4 years, but from only 25% of chicks aged 1-10 days. among the 104 strains isolated, we distinguished 14 lactobacillus species. the dominant spec ...201424607713
utility of thermostable xylanases of mycothermus thermophilus in generating prebiotic xylooligosaccharides.xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. in the present study, four neutral, thermostable xylanases (mtxyn11a, mtxyn11at, mtxyn11b, and mtxyn11c) from compost fungus mycothermus thermophilus cgmcc3.18119 were overexpressed in pichia pastoris gs115 and used to produce xylooligosaccharides from beechwood xylan. the enzymes showed similar enzymatic properties (maximal activities at ph 6.0-6.5 and 65 °c) but varied in catalytic efficiency and ...201728067041
construction of a constitutively expressed homo-fermentative pathway in lactobacillus brevis.lactobacillus brevis is a promising lactic acid producing strain that simultaneously utilizes glucose and xylose from lignocellulosic hydrolysate without carbon catabolic repression and inhibition. the production of by-products acetic acid and ethanol has been the major drawback of this strain. two genes, pfka (fructose-6-phosphate kinase [pfk]) and fbaa (fructose-1,6-biphosphate aldolase [fba]), that encode the key enzymes of the emp/glycolytic pathway from lactobacillus rhamnosus, were fused t ...201424728715
dynamics and rrna transcriptional activity of lactococci and lactobacilli during cheddar cheese ripening.cheddar cheese is a complex ecosystem where both the bacterial population and the cheese making process contribute to flavor and texture development. the aim of this study was to use molecular methods to evaluate the impact of milk heat treatment and ripening temperature on starter lactococci and non-starter lactic acid bacteria (nslab) throughout ripening of cheddar cheese. eight cheddar cheese batches were manufactured (four with thermized and four with pasteurized milk) and ripened at 4, 7 an ...201323850855
lactobacillus casei rhamnosus lcr35 in the management of functional constipation in children: a randomized trial.to assess the effectiveness of lactobacillus casei rhamnosus lcr35 (lcr35) in the management of functional constipation in children.201728284477
mg(2+) improves the thermotolerance of probiotic lactobacillus rhamnosus gg, lactobacillus casei zhang and lactobacillus plantarum p-8.food-related carbohydrates and proteins are often used as thermoprotectants for probiotic lactobacilli during industrial production and processing. however, the effect of inorganic salts is rarely reported. magnesium is the second-most abundant cation in bacteria, and commonly found in various foods. mg(2+) homeostasis is important in salmonella and has been reported to play a critical role in their thermotolerance. however, the role of mg(2+) in thermotolerance of other bacteria, in particular ...201728100014
network meta-analysis of probiotics to prevent respiratory infections in children and adolescents.probiotics have emerged as a promising intervention for the prevention of respiratory tract infections (rtis) in children. assess the effect of probiotics on prevention of rtis in children and adolescents. medline, embase, lilacs, scielo, cinahl, scopus, and web of science. key words: "respiratory tract infections" and probiotics. randomized controlled trials rct assessing the effect of probiotics on rtis in children and adolescents were included. two reviewers, working independently, to identif ...201728052594
lactobacillus casei subsp. rhamnosus septicaemia in three patients of the paediatric intensive care unit. 201628029469
human milk and mucosal lacto- and galacto-n-biose synthesis by transgalactosylation and their prebiotic potential in lactobacillus species.lacto-n-biose (lnb) and galacto-n-biose (gnb) are major building blocks of free oligosaccharides and glycan moieties of glyco-complexes present in human milk and gastrointestinal mucosa. we have previously characterized the phospho-β-galactosidase gnbg from lactobacillus casei bl23 that is involved in the metabolism of lnb and gnb. gnbg has been used here in transglycosylation reactions, and it showed the production of lnb and gnb with n-acetylglucosamine and n-acetylgalactosamine as acceptors, ...201727714445
survival and bioactivities of selected probiotic lactobacilli in yogurt fermentation and cold storage: new insights for developing a bi-functional dairy food.in previous work, we demonstrated that two probiotic strains, namely lactobacillus casei pra205 and lactobacillus rhamnosus pra331, produce fermented milks with potent angiotensin-converting enzyme (ace)-inhibitory and antioxidant activities. here, we tested these strains for the survivability and the release of antihypertensive and antioxidant peptides in yogurt fermentation and cold storage. for these purposes three yogurt batches were compared: one prepared using yogurt starters alone (lactob ...201627554146
the spxb gene as a target to identify lactobacillus casei group species in cheese.this study focused on the spxb gene, which encodes for pyruvate oxidase. the presence of spxb in the genome and its transcription could be a way to produce energy and allow bacterial growth during carbohydrate starvation. in addition, the activity of pyruvate oxidase, which produces hydrogen peroxide, could be a mechanism for interspecies competition. because this gene seems to provide advantages for the encoding species for adaptation in complex ecosystems, we studied spxb in a large set of che ...201627375244
can the development and autolysis of lactic acid bacteria influence the cheese volatile fraction? the case of grana padano.in this study, the relationship between the dynamics of the growth and lysis of lactic acid bacteria in grana padano cheese and the formation of the volatile flavor compounds during cheese ripening was investigated. the microbial dynamics of grana padano cheeses that were produced in two different dairies were followed during ripening. the total and cultivable lactic microflora, community composition as determined by length heterogeneity-pcr (lh-pcr), and extent of bacterial lysis using an intra ...201627299670
theoretical insight into the heat shock response (hsr) regulation in lactobacillus casei and l. rhamnosus.the understanding of the heat shock response (hsr) in lactobacilli from a regulatory point of view is still limited, though an increased knowledge on the regulation of this central stress response can lead to improvements in the exploitation of these health promoting microorganisms. therefore the aim of this in silico study, that is the first to be carried out for members of the lactobacillus genus, was predicting how hsr influences cell functions in the food associated and probiotic species lac ...201627142777
probiotics in critically ill children.gut microflora contribute greatly to immune and nutritive functions and act as a physical barrier against pathogenic organisms across the gut mucosa. critical illness disrupts the balance between host and gut microflora, facilitating colonization, overgrowth, and translocation of pathogens and microbial products across intestinal mucosal barrier and causing systemic inflammatory response syndrome and sepsis. commonly used probiotics, which have been developed from organisms that form gut microbi ...201627081478
genomic and functional characterization of the unusual plock 0919 plasmid harboring the spacba pili cluster in lactobacillus casei lock 0919.here, we report the extensive bioinformatic and functional analyses of the unusual plock 0919, a plasmid originating from the probiotic lactobacillus casei lock 0919 strain. this plasmid is atypical because it harbors the spacba-srtc gene cluster encoding spacba pili. we show that all other spacba-srtc sequences of the lactobacillus genus that have been previously described and deposited in genbank are present in the chromosomal dna. another important observation for plock 0919 is that the spacb ...201526637469
the use of date waste for lactic acid production by a fed-batch culture using lactobacillus casei subsp. rhamnosus.the production of lactic acid from date juice by lactobacillus caseisubsp. rhamnosus in batch and fed-batch cultures has been investigated. the fed-batch culture system gave better results for lactic acid production and volumetric productivity. the aim of this work is to determine the effects of the feeding rate and the concentration of the feeding medium containing date juice glucose on the cell growth, the consumption of glucose and the lactic acid production by lactobacillus casei subsp. rham ...201726413076
screening of immunomodulatory and adhesive lactobacillus with antagonistic activities against salmonella from fermented vegetables.the purpose of this study was to select strains of lactic acid bacteria (lab) by their in vitro adhesive and immunomodulatory properties for potential use as probiotics. in this study, 16 randomly selected lab strains from fermented vegetables (sauerkraut, bean and cabbage) were first screened for their tolerance to acid, bile salts, pepsin and pancreatin, bacterial inhibitory activities and abilities to adherence to caco-2 cells. then, 4 strains with the highest adhesion abilities were selected ...201526340935
impact of non-starter lactobacilli on release of peptides with angiotensin-converting enzyme inhibitory and antioxidant activities during bovine milk fermentation.this study aimed at evaluating non-starter lactobacilli (nslab) isolated from cheeses for their proteolytic activity and capability to produce fermented milk enriched in angiotensin-converting enzyme (ace)-inhibitory and antioxidant peptides. preliminarily, 34 nslab from parmigiano reggiano (pr) and 5 from pecorino siciliano cheeses were screened based on their capacity to hydrolyze milk proteins. two nslab strains from pr, lactobacillus casei pra205 and lactobacillus rhamnosus pra331, showed th ...201526187835
the dnaj gene as a molecular discriminator to differentiate among species and strain within the lactobacillus casei group.identifying lactobacillus casei and its closely related taxa at the species and strain level using only phenotypic and genotypic (16s rdna sequence homology analysis) techniques often yields inaccurate results. in this study, the dnaj chaperone gene was investigated as a molecular target for inter- and intraspecies discrimination within the lb. casei group as well as for the development of specific primers for species identification. the results showed that most of the examined strains could be ...201526050941
evaluation of synergistic interactions between cell-free supernatant of lactobacillus strains and amikacin and genetamicin against pseudomonas aeruginosa.the indiscriminate use of antibiotics in the treatment of infectious diseases can increase the development of antibiotic resistance. therefore, there is a big demand for new sources of antimicrobial agents and alternative treatments for reduction of antibiotic dosage required to decrease the associated side effects.201526034539
amelioration of ovalbumin induced allergic symptoms in balb/c mice by potentially probiotic strains of lactobacilli.to evaluate the antiallergic effect of newly characterised probiotic strains, lactobacillus fermentum nws29, lactobacillus casei nwp08 and lactobacillus rhamnosus nwp13, mice were divided into six experimental groups: control, ovalbumin (ova), nws29, nwp08, nwp13 and l. rhamnosus gg (lgg). mice were immunised and probiotics were administered via oral gavage followed by challenge with ova. after last challenge with ova, inflammatory cells in bronchoalveolar lavage fluid (balf), recruitment of inf ...201525869278
folate content and retention in commonly consumed vegetables in the south pacific.this paper reports the effect of boiling and frying on the retention of folate in commonly consumed fijian vegetables (drumstick leaves, taro leaves, bele leaves, amaranth leaves, fern/ota, okra and french bean). the folate content was determined by microbiological assay (lactobacillus casei rhamnosus) and tri-enzyme (protease, α-amylase and chicken pancreas conjugase) extraction treatment. the folate loss varied among the vegetables from 10-64% on boiling while 1-36% on frying. the higher folat ...201525842344
functional properties of peanut fractions on the growth of probiotics and foodborne bacterial pathogens.various compounds found in peanut (arachis hypogaea) have been shown to provide multiple benefits to human health and may influence the growth of a broad range of gut bacteria. in this study, we investigated the effects of peanut white kernel and peanut skin on 3 strains of lactobacillus and 3 major foodborne enteric bacterial pathogens. significant (p < 0.05) growth stimulation of lactobacillus casei and lactobacillus rhamnosus was observed in the presence of 0.5% peanut flour (pf) made from pe ...201525627431
high resolution melting analysis (hrm) as a new tool for the identification of species belonging to the lactobacillus casei group and comparison with species-specific pcrs and multiplex pcr.the correct identification and characterisation of bacteria is essential for several reasons: the classification of lactic acid bacteria (lab) has changed significantly over the years, and it is important to distinguish and define them correctly, according to the current nomenclature, avoiding problems in the interpretation of literature, as well as mislabelling when probiotic are used in food products. in this study, species-specific pcr and hrm (high-resolution melting) analysis were developed ...201525475306
molecular characterization of lactobacillus plantarum dmdl 9010, a strain with efficient nitrite degradation capacity.nitrites commonly found in food, especially in fermented vegetables, are potential carcinogens. therefore, limiting nitrites in food is critically important for food safety. a lactobacillus strain (lactobacillus sp. dmdl 9010) was previously isolated from fermented vegetables by our group, and is not yet fully characterized. a number of phenotypical and genotypical approaches were employed to characterize lactobacillus sp. dmdl 9010. its nitrite degradation capacity was compared with four other ...201425423449
impact of different cryoprotectants on the survival of freeze-dried lactobacillus rhamnosus and lactobacillus casei/paracasei during long-term storage.the production of long shelf-life highly concentrated dried probiotic/starter cultures is of paramount importance for the food industry. the aim of the present study was to evaluate the protective effect of glucose, lactose, trehalose, and skim milk applied alone or combined upon the survival of potentially probiotic lactobacillus rhamnosus ctc1679, lactobacillus casei/paracasei ctc1677 and l. casei/paracasei ctc1678 during freeze-drying and after 39 weeks of storage at 4 and 22 °c. immediately ...201525380798
the efficacy of ampicillin and lactobacillus casei rhamnosus in the active management of preterm premature rupture of membranes remote from term.we aimed to investigate the treatment efficacy of ampicillin prophylaxis accompanied by lactobacillus casei rhamnosus over the latency period following preterm premature rupture of membranes (pprom).201425210439
a new risk factor for neonatal vancomycin-resistant enterococcus colonisation: bacterial probiotics.vancomycin-resistant enterococcus (vre) colonisation can be controlled with strict adherence to infection control measures. we describe a vre outbreak coincident with bacterial probiotic trial. relationship between probiotic and vre colonisation, and other possible risk factors were investigated.201525163639
in vitro evaluation of the probiotic and functional potential of lactobacillus strains isolated from fermented food and human intestine.this study aims to evaluate the functional and probiotic characteristics of eight indigenous lactobacillus strains in vitro. the selected lactobacilli include strains of lactobacillus casei subsp. casei, lactobacillus salivarius subsp. salicinius, lactobacillus fermentum, lactobacillus plantarum, lactobacillus delbrueckii subsp. lactis, lactobacillus delbrueckii subsp. bulgaricus, and lactobacillus rhamnosus. all strains tolerated both ph 2 for 3 h and 1% bile salt for 24 h. the strains cicc 231 ...201425046742
lactobacillus rhamnosus gg suspected infection in a newborn with intrauterine growth restriction.a disseminated lactobacillus rhamnosus gg atcc 53103 infection was suspected in a 6 day-old newborn with intrauterine growth restriction (iugr) symptoms, treated empirically with antibiotics and given l. rhamnosus gg with the aim of preventing antibiotic-associated gastrointestinal complications. the level of c-reactive protein on day 5 compared with day 2 was increased in spite of negative urine and cerebrospinal fluid cultures. the blood sampled on day 6 was found to be positive for lactobacil ...201425035097
association of levels of antibodies from patients with inflammatory bowel disease with extracellular proteins of food and probiotic bacteria.inflammatory bowel disease (ibd) is an autoimmune disease characterized by a chronic inflammation of the gastrointestinal tract mucosa and is related to an abnormal immune response to commensal bacteria. our aim of the present work has been to explore the levels of antibodies (igg and iga) raised against extracellular proteins produced by lab and its association with ibd. we analyzed, by western-blot and elisa, the presence of serum antibodies (iga and igg) developed against extracellular protei ...201424991549
β-glucosidase activity and bioconversion of isoflavones during fermentation of soymilk.lactobacillus rhamnosus c6 strain showed higher β-glucosidase activity as well as biotransformation of isoflavones from glycones (daidzin and genistin) to aglycones (daidzein and genistein) in soymilk. however, l. rhamnosus c2 and lactobacillus casei ncdc297 also exhibited similar activity during soymilk fermentation. these three strains can be selected for the development of functional fermented soy foods enriched with aglycone forms of isoflavones, such as soy yoghurt, soy cheese, soy beverage ...201524838442
the effect of five probiotic lactobacilli strains on the growth and biofilm formation of streptococcus mutans.to compare the effects of five probiotic lactobacilli strains on the growth and biofilm formation of streptococcus mutans (ms).201524806217
[promising immunonutrients in general medical practice].the review gives data on prospects for using agents based on probiotics and trace elements for immunomodulation. it presents information on that a lactobacillus casei rhamnosus 35-based probiotic is able to reduce the incidence and severity of viral and bacterial infections. the potential of zinc and selenium as immunotropic agents is shown.201424772516
characterization of nitrite degradation by lactobacillus casei subsp. rhamnosus lcr 6013.nitrites are potential carcinogens. therefore, limiting nitrites in food is critically important for food safety. the nitrite degradation capacity of lactobacillus casei subsp. rhamnosus lcr 6013 was investigated in pickle fermentation. after lcr 6013 fermentation for 120 h at 37°c, the nitrite concentration in the fermentation system was significantly lower than that in the control sample without the lcr 6013 strain. the effects of nacl and vc on nitrite degradation by lcr 6013 in the de man, r ...201424755671
genome sequence of the probiotic strain lactobacillus rhamnosus (formerly lactobacillus casei) lock908.lactobacillus rhamnosus lock908, a patented probiotic strain (polish patent no. 209987), was isolated from the feces of a healthy 6-year-old girl. here, we present the complete genome sequence of lock908 and identify genes likely to be involved in the biosynthesis of exopolysaccharides (epss).201424558250
nutritionally enhanced fermented sausages as a vehicle for potential probiotic lactobacilli delivery.the suitability of three potential probiotic lactobacilli strains (lactobacillus casei ctc1677, l. casei ctc1678 and lactobacillus rhamnosus ctc1679), previously isolated from infants' faeces and characterized, and three commercial probiotic strains (lactobacillus plantarum 299v, l. rhamnosus gg and l. casei shirota) was assessed during the manufacture of low-acid fermented sausages (fuets) with reduced na(+) and fat content. the inoculated strains were successfully monitored by rapd-pcr during ...201424211552
comparison of fecundity and offspring immunity in zebrafish fed lactobacillus rhamnosus cicc 6141 and lactobacillus casei bl23.to increase the knowledge of probiotic effects on zebrafish (danio rerio), we compare the effects of two probiotic strains, lactobacillus rhamnosus cicc 6141 (a highly adhesive strain) and lactobacillus casei bl23 (a weakly adhesive strain), on zebrafish reproduction and their offsprings' innate level of immunity to water-borne pathogens. during probiotics treatments from 7 to 28 days, both the lactobacillus strains, and especially l. casei bl23, significantly increased fecundity in zebrafish: h ...201424129154
not only osmoprotectant: betaine increased lactate dehydrogenase activity and l-lactate production in lactobacilli.lactobacilli are commonly used for industrial production of polymer-grade l-lactic acid. the present study tested the tween 80 alternative betaine in l-lactate production by several industrial lactobacilli. in flask fermentation of lactobacillus casei, lactobacillus buchneri, lactobacillus lactis and lactobacillus rhamnosus, the betaine addition (2g/l) had similar osmoprotectant effect with tween 80 but had increased the lactate dehydrogenase activities and l-lactate production than tween 80 con ...201324035452
diversity and dynamic of lactic acid bacteria strains during aging of a long ripened hard cheese produced from raw milk and undefined natural starter.the aim of this study was to explore diversity and dynamic of indigenous lab strains associated with a long ripened hard cheese produced from raw milk and undefined natural starter such as pdo grana padano cheese. samples of milk, curd, natural whey culture and cheeses (2nd, 6th, 9th and 13th months of ripening) were collected from 6 cheese factories in northern italy. dna was extracted from each sample and from 194 lab isolates. trna(ala)-23s rdna-rflp was applied to identify isolates. strain d ...201324010599
effect of chestnut extract and chestnut fiber on viability of potential probiotic lactobacillus strains under gastrointestinal tract conditions.the main challenge to probiotics, during their passage through the gastrointestinal tract, are the acidic gastric secretions of the stomach, and the bile salts released into the duodenum. the survival of the strains, in this phase, is strongly influenced by the food used for their delivery. this work is part of a project studying the development of novel food processes, based on the use of chestnuts from cultivar "castagna di montella". in detail, the effect of indigestible chestnut fiber and of ...201324010594
genome sequence of the probiotic strain lactobacillus rhamnosus (formerly lactobacillus casei) lock900.lactobacillus rhamnosus lock900 fulfills the criteria required for probiotic strains. in this study, we report a whole-genome sequence of this isolate and compare it with other l. rhamnosus complete genome sequences already published.201323950137
synbiotic impact of tagatose on viability of lactobacillus rhamnosus strain gg mediated by the phosphotransferase system (pts).synbiotics, the combination of prebiotics and probiotics, has been shown to produce synergistic effects that promote gastrointestinal well-being of host. tagatose is a low calorie food ingredient with putative health-promoting benefits. herein, we investigated its synbiotic impact on the viability of lactobacillus casei 01 and lactobacillus rhamnosus strain gg and the potential mechanism involved. tagatose, as a synbiotic substrate, enhanced the growth of l. casei 01 and l. rhamnosus strain gg c ...201323764214
nonstarter lactic acid bacteria volatilomes produced using cheese components.in long-ripened cheese, flavor formation occurs during ripening. the metabolism of lactic acid bacteria (lab) leads to the production of different compounds that contribute to the flavor of cheese. the contribution of lab to the formation of cheese flavor has previously been studied. however, the specific nonstarter lab (nslab) metabolic reactions in ripened cheese that lead to the formation of flavor compounds remain unclear. in ripened cheese, the nutrient sources available include small pepti ...201323684038
significant effect of ca2+ on improving the heat resistance of lactic acid bacteria.the heat resistance of lactic acid bacteria (lab) has been extensively investigated due to its highly practical significance. reconstituted skim milk (rsm) has been found to be one of the most effective protectant wall materials for microencapsulating microorganisms during convective drying, such as spray drying. in addition to proteins and carbohydrate, rsm is rich in calcium. it is not clear which component is critical in the rsm protection mechanism. this study investigated the independent ef ...201323617813
comparative genomic and functional analysis of lactobacillus casei and lactobacillus rhamnosus strains marketed as probiotics.four lactobacillus strains were isolated from marketed probiotic products, including l. rhamnosus strains from vifit (friesland campina) and idoform (ferrosan) and l. casei strains from actimel (danone) and yakult (yakult honsa co.). their genomes and phenotypes were characterized and compared in detail with l. casei strain bl23 and l. rhamnosus strain gg. phenotypic analysis of the new isolates indicated differences in carbohydrate utilization between l. casei and l. rhamnosus strains, which co ...201323315726
stable integration and expression of heterologous genes in several lactobacilli using an integration vector constructed from the integrase and attp sequences of phage φat3 isolated from lactobacillus casei atcc 393.an integration vector capable of stably integrating and maintaining in the chromosomes of several lactobacilli over hundreds of generations has been constructed. the major integration machinery used is based on the φat3 integrase (int) and attp sequences determined previously. a novel core sequence located at the 3' end of the trna(leu) gene is identified in lactobacillus fermentum atcc 14931 as the integration target by the integration vector though most of such sequences found in other lactoba ...201323064454
functional analysis of the lactobacillus casei bl23 sortases.sortases are a class of enzymes that anchor surface proteins to the cell wall of gram-positive bacteria. lactobacillus casei bl23 harbors four sortase genes, two belonging to class a (srta1 and srta2) and two belonging to class c (srtc1 and srtc2). class c sortases were clustered with genes encoding their putative substrates that were homologous to the spaefg and spacba proteins that encode mucus adhesive pili in lactobacillus rhamnosus gg. twenty-three genes encoding putative sortase substrates ...201223042174
fermentation of sugars and sugar alcohols by plaque lactobacillus strains.the objective was to analyse the ability of lactobacillus strains isolated from supragingival plaque of subjects with hyposalivation and from healthy controls to ferment sugars and sugar alcohols.201322956128
vaginal impact of the oral administration of total freeze-dried culture of lcr 35 in healthy women.the use of probiotics in the prevention or treatment of some vaginal infections has been the subject of numerous studies. to assess the presence of lactobacillus casei rhamnosus (lcr35) in the vagina after an oral administration, an open randomised pilot study was conducted on 20 healthy women of child-bearing age. materials and methods. 2 groups of 10 women were given a 28-day oral course, that is, at least 108 cfu/day (group 1) or 2 × 108 cfu/day (group 2) of lcr35. nugent score and vaginal sc ...201222701297
inventory of non starter lactic acid bacteria from ripened parmigiano reggiano cheese as assessed by a culture dependent multiphasic approach.the objective of this study was to investigate microbial species diversity and strain complexity of the cultivable non starter lactic acid bacteria (nslab) occurring in 31 ripened parmigiano reggiano (pr) cheeses. dereplication of 127 lactobacilli isolates by (gtg)(5)-pcr fingerprinting yielded a total of 51 genotypes. phylogenetic relatedness of all the genotypes with known lactobacillus species was determined by a novel combined amplified 16s rdna restriction analysis (16s-ardra), species-spec ...201222626626
efficacy and safety profile of lcr35 complete freeze-dried culture in irritable bowel syndrome: a randomized, double-blind study.to assess the effects and safety of lactobacillus casei rhamnosus lcr35 complete freeze-dried culture (lcr35) in patients suffering from irritable bowel syndrome (ibs).201222563194
analysis of the peptidoglycan hydrolase complement of lactobacillus casei and characterization of the major γ-d-glutamyl-l-lysyl-endopeptidase.peptidoglycan (pg) is the major component of gram positive bacteria cell wall and is essential for bacterial integrity and shape. bacteria synthesize pg hydrolases (pghs) which are able to cleave bonds in their own pg and play major roles in pg remodelling required for bacterial growth and division. our aim was to identify the main pghs in lactobacillus casei bl23, a lactic acid bacterium with probiotic properties.the pgh complement was first identified in silico by amino acid sequence similarit ...201222384208
manufacture of fior di latte cheese by incorporation of probiotic lactobacilli.this work aimed to select heat-resistant probiotic lactobacilli to be added to fior di latte (high-moisture cow milk mozzarella) cheese. first, 18 probiotic strains belonging to lactobacillus casei, lactobacillus delbrueckii ssp. bulgaricus, lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus reuteri were screened. resistance to heating (65 or 55°c for 10 min) varied markedly between strains. adaptation at 42°c for 10 min increased the heat resistance at ...201222281315
lactobacilli differentially modulate mtor and wnt/ β-catenin pathways in different cancer cell lines.lactobacilli are a group of beneficial bacteria whose anti cancer effects have been evaluated in different cancer cell lines as well as animal models and human subjects. such anti cancer effects can be exerted via different mechanisms such as modulation of immune response as well as inhibition of pathogens colonization. in addition, lactobacilli have direct cytotoxic effects against cancer cells which may be exerted through modulation of expression cancer related pathways.201627703648
in vitro adherence of lactobacillus strains isolated from the vaginas of healthy iranian women.the lactobacilli are a part of the bacterial flora of the human vagina. detection of normal lactobacillus species in the vaginas of healthy women in different geographical locations, and evaluation of their specific properties, can aid in the selection of the best species for preventing sexually transmitted diseases in the future. this study was performed to isolate and identify the lactobacillus species in the vaginas of healthy women and to evaluate the adherence of these lactobacilli to vero ...201627562422
dual anti-metastatic and anti-proliferative activity assessment of two probiotics on hela and ht-29 cell lines.lactobacilli are a group of probiotics with beneficial effects on prevention of cancer. however, there is scant data in relation with the impacts of probiotics in late-stage cancer progration, especially metastasis. the present original work was aimed to evaluate the anti-metastatic and anti-proliferative activity of lactobacillus rhamnosus supernatant (lrs) and lactobacillus crispatus supernatant (lcs) on the human cervical and colon adenocarcinoma cell lines (hela and ht-29, respectively).201727551673
lactobacilli modulate hypoxia-inducible factor (hif)-1 regulatory pathway in triple negative breast cancer cell line.hypoxia-inducible factor (hif)-1 plays an essential role in the body's response to low oxygen concentrations and regulates expression of several genes implicated in homeostasis, vascularization, anaerobic metabolism as well as immunological responses. increased levels of hif-1α are associated with increased proliferation and more aggressive breast tumor development. lactobacilli have been shown to exert anti-cancer effects on several malignancies including breast cancer. however, the exact mecha ...201727540529
glutamine synthetase and glucose-6-phosphate isomerase are adhesive moonlighting proteins of lactobacillus crispatus released by epithelial cathelicidin ll-37.glutamine synthetase (gs) and glucose-6-phosphate isomerase (gpi) were identified as novel adhesive moonlighting proteins of lactobacillus crispatus st1. both proteins were bound onto the bacterial surface at acidic phs, whereas a suspension of the cells to ph 8 caused their release into the buffer, a pattern previously observed with surface-bound enolase and glyceraldehyde-3-phosphate dehydrogenase (gapdh) of l. crispatus. the ph shift was associated with a rapid and transient increase in cell ...201222389474
development of antioxidant rich fruit supplemented probiotic yogurts using free and microencapsulated lactobacillus rhamnosus culture.the present study reports the preparation of probiotic yogurt using lactobacillus rhamnosus. the standard starter cultures used for yogurt fermentation were streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus and obtained from ndri, karnal, india. the prepared yogurt was supplemented with fruit pulp (10 % w/v) of apricot, raspberries, plum and jamun. these fruits were rich in antioxidant property as observed by dpph, nitric oxide radical scavenging and ferric reducing anti ...201626787986
novel method based on chromogenic media for discrimination and selective enumeration of lactic acid bacteria in fermented milk products.microbial analyses of fermented milk products require selective methods to discriminate between close species simultaneously present in high amounts. a culture-based method combining novel chromogenic agar media and appropriate incubation conditions was developed to enumerate lactic acid bacteria (lab) strains in fermented milk. m1 agar, containing two chromogenic substrates, allowed selective enumeration of lactobacillus rhamnosus, two strains of lactobacillus paracasei subsp. paracasei and str ...201626742619
effects of fermentation with lactobacillus rhamnosus gg on product quality and fatty acids of goat milk yogurt.the effect of fermentation with lactobacillus rhamnosus gg on the product quality of goat milk yogurt using traditional yogurt starter was studied through single-factor experiments and orthogonal experiments. the optimum fermentation condition was evaluated by the titratable acidity of goat milk yogurt, water-retaining capability, sensory score, and texture properties; the fatty acids of the fermented goat milk were determined by a gas chromatograph. results indicate that high product quality of ...201626601583
new lactic acid bacterial strains from traditional mongolian fermented milk products have altered adhesion to porcine gastric mucin depending on the carbon source.attachment of lactic acid bacteria to the mucosal surface of the gastrointestinal tract is a major property of probiotics. here, we examined the ability of 21 lactic acid bacterial strains isolated from traditional fermented milk products in mongolia to adhere to porcine gastric mucin in vitro. higher attachment was observed with lactobacillus delbrueckii subsp. bulgaricus strains 6-8 and 8-1 than with lactobacillus rhamnosus gg (positive control). lactococcus lactis subsp. cremoris strain 7-1 a ...201525186082
diversity and functional characterization of lactobacillus spp. isolated throughout the ripening of a hard cheese.the aim of this work was to study the lactobacillus spp. intra- and inter- species diversity in a piedmont hard cheese made of raw milk without thermal treatment and without addition of industrial starter, and to perform a first screening for potential functional properties. a total of 586 isolates were collected during the cheese production and identified by means of molecular methods: three hundred and four were identified as lactobacillus rhamnosus, two hundred and forty as lactobacillus helv ...201424819414
the microfloras and sensory profiles of selected protected designation of origin italian cheeses.approximately 39 italian cheeses carry protected designation of origin (pdo) status. these cheeses differ in their manufacturing technology and the microbial flora which comprise the finished products. the evolution of lactic microflora in cheeses with pdo status is of particular interest because the biochemical activities of these organisms participate in cheesemaking and may play an acknowledged role in the development of organoleptic characteristics during ripening. nonstarter lactic acid bac ...201426082116
oral administration of lactobacillus delbrueckii subspecies bulgaricus oll1073r-1 suppresses inflammation by decreasing interleukin-6 responses in a murine model of atopic dermatitis.the oral intake of lactobacillus spp. can provide beneficial effects to the host by modulating the immune response. atopic dermatitis (ad) is an allergic inflammatory disease mediated by various immune responses. in this study, we examined the effect of a lactobacillus strain, lactobacillus delbrueckii ssp. bulgaricus oll1073r-1 (oll1073r-1), on ad development in a murine model of ad that was developed by the topical application of mite antigen in nc/nga mice. the oral intake of heat-killed oll1 ...201323548305
dietary sugar utilisation by putative oral probiotics.probiotic consumption and repeated probiotic intake have shown promising results as adjunct therapies in prevention and alleviation of some chronic disease conditions in the gastrointestinal tract. recent evidence suggests that probiotics may also be beneficial in preventing oral diseases. an efficient probiotic candidate in the mouth, however, should not impose any risk to oral tissues, such as acid demineralisation of tooth enamel because of sugar fermentation. the aim of the present in vitro ...201222968411
anti-diabetic effects of lactic acid bacteria in normal and type 2 diabetic mice.the antidiabetic effects of lactic acid bacteria were investigated using mice. in experiment 1, normal icr mice were loaded with sucrose or starch with or without viable lactobacillus rhamnosus gg cells. gg significantly inhibited postprandial blood glucose levels when administered with sucrose or starch. in experiment 2, kk-a(y) mice, a model of genetic type 2 diabetes, were given a basal diet containing viable gg cells or viable lactobacillus delbrueckii subsp. bulgaricus cells for 6 weeks. vi ...201222962525
regulation of tlr4, p38 mapkinase, iκb and mirnas by inactivated strains of lactobacilli in human dendritic cells.strain specific properties of probiotics in providing supportive health effects in the immune system and the gastrointestinal tract have been widely investigated in vivo and in vitro. however, the underlying responsible mechanism is poorly described. by unravelling the probiotic-induced responses in a complex network of interacting signalling pathways, we investigated the effect of heat-inactivated lactobacillus rhamnosus gg (lgg) and lactobacillus delbrueckii subsp. bulgaricus (l.del) on the ex ...201222476320
the lactobacillus rhamnosus and lactobacillus fermentum strains from human biotopes characterized with mlst and toxin-antitoxin gene polymorphism.the diversity of lb. rhamnosus and lb. fermentum strains isolated from feces, saliva, and the vaginal cavity of 18-22-year-old healthy women residing in central regions of the russian federation has been characterized. the results obtained using multilocus sequence typing were identical to those obtained with the analysis of genetic and genomic polymorphism in ta systems. different as well as identical lb. rhamnosus and lb. fermentum sequence types (st) were isolated from various parts of the bo ...201728213763
improvement in glucose tolerance and insulin sensitivity by probiotic strains of indian gut origin in high-fat diet-fed c57bl/6j mice.diabetes and obesity are characterized by glucose intolerance, fat deposition, inflammation, and dyslipidemia. recent reports postulated that distinct gut microbiota alterations were observed in obese/diabetic subjects and modulating gut microbiota beneficially through specific probiotics could be a potential therapeutic option for type 2 diabetes/obesity. therefore, we attempted to study the efficacy of probiotics of indian gut origin (lactobacillus plantarum mtcc5690 and lactobacillus fermentu ...201627757592
lactobacillus and leuconostoc volatilomes in cheese conditions.new strains are desirable to diversify flavour of fermented dairy products. the objective of this study was to evaluate the potential of leuconostoc spp. and lactobacillus spp. in the production of aroma compounds by metabolic fingerprints of volatiles. eighteen strains, including five lactobacillus species (lactobacillus fermentum, lactobacillus helveticus, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus sakei) and three leuconostoc species (leuconostoc citreum, leuconostoc lact ...201626685674
assessment and comparison of probiotic potential of four lactobacillus species isolated from feces samples of iranian infants.the probiotic potential of lactobacillus species isolated from infant feces was investigated. for this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (caco-2 cells) of four common lactobacillus species (lactobacillus paracasei [n = 15], lactobacillus rhamnosus [n = 45], lactobacillus gasseri [n = 20] and lactobacillus fermentum [n = 18]) were assessed. most isolates that which ...201626648174
probiotic strains influence on infant microbiota in the in vitro colonic fermentation model gis1.the main goal of our study was to evaluate the effect of the individual administration of five lyophilized lactic acid bacteria strains (lactobacillus fermentum 428st, lactobacillus rhamnosus e4.2, lactobacillus plantarum fca3, lactobacillus sp. 34.1, weissella paramesenteroides ft1a) against the in vitro simulated microbiota of the human colon using the gis1 system. the influence on the metabolic activity was also assessed by quantitative determination of proteins and polysaccharides at each se ...201526543268
in vitro probiotic characterization of lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens.in this study, we evaluated the probiotic properties of lactobacillus plantarum, lactobacillus pentosus, and lactobacillus fermentum strains isolated from fermented radish. all the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to caco-2 cells compared with the probiotic strain lactobacillus rhamnosus gg. the strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. furthermor ...201526382558
probiotic characteristics of lactobacillus fermentum strains isolated from tulum cheese.the aim of this study was to characterize the probiotic characteristics of lactobacillus fermentum strains isolated from tulum cheese. seven l. fermentum strains were selected among the isolated and identified lactobacillus strains due to their abundance. when the gastric condition was considered, l. fermentum lp3 and lp4 were able to tolerate ph 2.5 and 1% bile salt. all l. fermentum strains had similar enzymatic activity and antibiotic resistance pattern but the highest antagonistic effect was ...201425270832
effect of two probiotic strains of lactobacillus on in vitro adherence of listeria monocytogenes, streptococcus agalactiae, and staphylococcus aureus to vaginal epithelial cells.the lactobacilli probiotics maintain a normal vaginal biota and prevent disease recurrence. this microorganisms form a pellicle on the vaginal epithelium that acts as a biologic barrier against colonization by pathogenic bacteria. in this paper were realized assays of exclusion, competition, and displacement. for these test, vaginal epithelial cells, two strains of lactobacilli and pathogenic bacteria (staphylococcus aureus, streptococcus agalactiae and listeria monocytogenes) were used. the lac ...201424469557
the effect of growth media and physical treatments on the adhesion properties of canine probiotics.the manufacturing processes have been reported to influence the properties of probiotics with potential impact on health properties. the aim was to investigate the effect of different growth media and inactivation methods on the properties of canine-originated probiotic bacteria alone and in combination mixture.201323617818
isolation of potential probiotic lactobacillus rhamnosus strains from traditional fermented mare milk produced in sumbawa island of indonesia.to explore potential probiotics in the traditional foods of indonesia, fermented mare milk produced in sumbawa island was investigated in this study. gram stain, catalase activity, gas production, cell morphology, carbohydrate utilization pattern, and 16s rdna sequencing were performed to identify isolated lactic acid bacteria. to assess their probiotic ability, tolerance of low ph, bile salts, artificial gastrointestinal fluids, and adhesion properties to extracellular matrices, were examined. ...201223047104
antifungal activity of two lactobacillus strains with potential probiotic properties.aflatoxin (highly toxic and carcinogenic secondary metabolites produced by fungi) contamination is a serious problem worldwide. modern agriculture and animal production systems need to use high-quality and mycotoxin-free feedstuffs. the use of microorganisms to preserve food has gained importance in recent years due to the demand for reduced use of chemical preservatives by consumers. lactic acid bacteria are known to produce various antimicrobial compounds that are considered to be important in ...201222497448
synergistic effect between two bacteriocin-like inhibitory substances produced by lactobacilli strains with inhibitory activity for streptococcus agalactiae.group b streptococci (gbs) are bacterial species that colonize the vagina in pregnant women and as such may cause serious infections in neonates that passed through the birth channel. the objective of this work was to study the inhibitory activities produced by each bacteriocin-like inhibitory substance (blis) of lactobacillus rhamnosus l60 and lactobacillus fermentum l23, and the effects of the combined blis-es of these lactobacilli on gbs. the interactions between the blis-es were assessed by ...201222231454
probiotics modulate gut microbiota and health status in japanese cedar pollinosis patients during the pollen season.japanese cedar pollinosis (jcp) is a challenging public health problem in japan. altered gut microbiota is associated with several diseases, including allergic diseases. however, only a few studies have focused on jcp and the underlying mechanisms for probiotic effects remain unclear. in addition, this study is the first observation of the correlation between the gut microbiota and blood lipid in jcp.201627412706
vaginal colonisation by probiotic lactobacilli and clinical outcome in women conventionally treated for bacterial vaginosis and yeast infection.the aim of this study was to investigate the colonisation by lactobacilli and clinical outcome in women with bacterial vaginosis (bv) and recurrent vulvovaginal candidiasis (r-vvc) receiving antibiotic or anti-fungal treatment in combination with the probiotic ecovag® capsules.201526137971
administration of probiotics lactobacillus rhamnosus gg and lactobacillus gasseri k7 during pregnancy and lactation changes mouse mesenteric lymph nodes and mammary gland microbiota.the milk and mammary gland (mg) microbiome can be influenced by several factors, such as mode of delivery, breastfeeding, maternal lifestyle, health status, and diet. an increasing number of studies show a variety of positive effects of consumption of probiotics during pregnancy and breastfeeding on the mother and the newborn. the aim of this study was to investigate the effect of oral administration of probiotics lactobacillus gasseri k7 (lk7) and lactobacillus rhamnosus gg (lgg) during pregnan ...201525622869
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