| from probiotics to therapeutics: another step forward? | preclinical studies with probiotics continue to unravel mechanisms of cytoprotection and suggest that approaches utilizing microbial products as therapeutics in acute and chronic gastrointestinal disorders could be effective. however, clinical trials using these bacteria have thus far been inconsistent. in this issue of the jci, yan et al. describe a novel mechanism of cytoprotection by p40, a soluble product of lactobacillus rhamnosus gg, mediated via egfr. the efficacy of p40 in three models o ... | 2011 | 21606604 |
| comparison of serum and red blood cell folate microbiologic assays for national population surveys. | three laboratories participated with their laboratory-specific microbiologic growth assays (ma) in the nhanes 2007-2008 to assess whether the distributions of serum (n = 2645) and rbc folate (n = 2613) for the same one-third sample of participants were comparable among laboratories. laboratory (l) 2 produced the highest and l1 the lowest serum and rbc folate geometric means (nmol/l) in the nhanes sample (serum: l1, 39.5; l2, 59.2; l3, 47.7; and rbc: l1, 1120; l2, 1380; l3, 1380). each laboratory ... | 2011 | 21613453 |
| comparative proteome cataloging of lactobacillus rhamnosus strains gg and lc705. | the present study reports an in-depth proteome analysis of two lactobacillus rhamnosus strains, the well-known probiotic strain gg and the dairy strain lc705. we used gelc-ms/ms, in which proteins are separated using 1-de and identified using nanolc-ms/ms, to generate high-quality protein catalogs. to maximize the number of identifications, all data sets were searched against the target databases using two search engines, mascot and paragon. as a result, over 1,600 high-confidence protein identi ... | 2011 | 21615180 |
| folate content and retention in selected raw and processed foods. | adequate intake of folate reduced the risk of abnormalities in early embryonic brain development such as the risk of malformations of the embryonic brain/spinal cord, collectively referred to as neural tube defects (ntds). folate is extremely sensitive to destruction by heat, oxidation and uv light. the purpose of this study was to evaluate the use of different extraction procedures and enzymatic treatment to determine folate concentrations in variety of foods using a microbiological assay (ma) ... | 2010 | 21612148 |
| probiotics and dietary counselling targeting maternal dietary fat intake modifies breast milk fatty acids and cytokines. | purpose: breast milk fatty acids possess immunomodulatory properties, and new intervention strategies beyond supplementation of maternal diet with single oils are called for. the objective of the present study was to evaluate the effect of dietary intervention during pregnancy and breastfeeding on breast milk fatty acid and cytokine composition. methods: pregnant women were randomised into three study groups: dietary intervention with probiotics (diet/probiotic) or with placebo (diet/placebo) an ... | 2011 | 21626296 |
| a randomized, double-blinded, placebo-controlled pilot study of probiotics in active rheumatoid arthritis. | background: to examine the effect of probiotics as adjunctive therapy for the treatment of rheumatoid arthritis (ra). a sample size of 30 subjects was calculated to determine a moderate effect.<br /> material/methods: a three month double-blind, placebo-controlled study was performed using probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 capsules administered orally. inclusion criteria required at least 4 swollen and 4 tender joints and stable medications with no steroids f ... | 2011 | 21629190 |
| effect of 25 weeks probiotic supplementation on immune function of hiv patients. | introduction: studies with a follow-up of < 8 weeks have indicated immune-preserving effects of yogurt probiotic supplementation among hiv patients. to evaluate the impact of 25 weeks use of dried, encapsulated probiotics, a randomized, double blind, controlled study was undertaken on 65 women who were naïve to anti-retroviral treatment. results: ten participants were excluded post-randomization due to non-eligibility. thirty participants were assigned placebo, of whom 25 completed the study ver ... | 2011 | 21637031 |
| an evaluation of the ability of the probiotic strain lactobacillus rhamnosus gg to eliminate the gastrointestinal carrier state of vancomycin-resistant enterococci in colonized children. | goals:: to evaluate the efficacy of lactobacillus rhamnosus gg (lgg) supplementation in eliminating the gastrointestinal carrier state of vancomycin-resistant enterococci (vre) in colonized children, and to evaluate the affect of the probiotic on lactobacillus spp. counts in the gastrointestinal tract. study:: a randomized, single-blind, placebo-controlled study. children (0 to 18 y old) hospitalized at the wards of the children's hospital who were diagnosed with gastrointestinal carrier state o ... | 2011 | 21814146 |
| coupling two sizes of cstr-type bioreactors for sequential lactic acid and xylitol production from hemicellulosic hydrolysates of vineshoot trimmings. | this study develops a system for the efficient valorisation of hemicellulosic hydrolysates of vineshoot trimmings. by connecting two reactors of 2l and 10l, operational conditions were set up for the sequential production of lactic acid and xylitol in continuous fermentation, considering the dependence of the main metabolites and fermentation parameters on the dilution rate. in the first bioreactor, lactobacillus rhamnosus consumed all the glucose to produce lactic acid at 31.5°c, with 150rpm an ... | 2011 | 21807126 |
| a comparative evaluation of probiotics on salivary mutans streptococci counts in indian children. | probiotics and their effect on oral health have been a focus of numerous trials in recent times. no documented trials have been reported from developing countries such as india with its focus on probiotic use, especially in the paediatric population. the aim of this study is to evaluate the effect of probiotics on salivary mutans streptococci (ms) counts of children using the two commercially and widely available preparations and to explore their anti-caries potential. | 2011 | 21806906 |
| lactobacillus rhamnosus gg secreting an antigen and interleukin-2 translocates across the gastrointestinal tract and induces an antigen specific immune response. | lactobacillus rhamnosus strain gg (lgg) is a probiotic organism. in this present study, lgg that express the green fluorescence protein (lgg-gfp) and interleukin-2 (il2) and gfp as a fusion protein (lgg-il2-gfp) were used to examine bacterial uptake and the immune response induced by oral immunization. using transmission electron microscopy (tem) to examine the intestinal tissue, the lactobacilli were localized in m cells and in venules. after oral immunization, most of the bacteria were excrete ... | 2011 | 21806675 |
| in vitro mutagen binding and antimutagenic activity of human lactobacillus rhamnosus 231. | in vitro mutagen binding ability of human lactobacillus rhamnosus 231 (lr 231) was evaluated against acridine orange (ao), n-methyl-n'-nitro-n-nitrosoguanidine (mnng), 2-amino-3, 8-dimethylimidazo-[4,5-f]-quinoxaline (meiqx) and 4-nitro-o-phenylenediamine (npd). binding of ao by lr 231 is due to adsorption, thereby leading to removal of mutagen in solution and is instantaneous, ph- and concentration-dependent. whereas, binding of mnng and meiqx by lr 231 results into biotransformation leading to ... | 2011 | 21777684 |
| the effect of temperature and ph gradients on lactobacillus rhamnosus gene expression of stress-related genes. | in this study, lactobacillus rhamnosus, a renowned probiotic, was cultivated in fluctuating environment. base gradients caused by a ph control in an industrial process and temperature gradients caused by uneven heating were simulated with a scale-down method. a ph gradient was created in a plug flow reactor (pfr). expression of ph stress-related genes (atpa, aldb, cfa, groel, hrca and psts) were studied as a relative gene expression study using ldhd as a reference gene. expression measurements w ... | 2011 | 21779891 |
| inhibitory effect of oral lactobacillus against oral pathogens. | aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ... | 2011 | 21801186 |
| in vitro selection of antibiotic resistance in the probiotic strain lactobacillus rhamnosus gg atcc 53103. | antibiotic resistance in probiotic strains is a matter of interest due to the increase in consumption of probiotic products. many studies have evaluated the antibiotic susceptibility of lactobacilli or the presence of resistance determinants, while knowledge on selection of resistance during exposure to antibiotics is still limited. our aim was to evaluate the behavior of lactobacillus rhamnosus gg atcc 53103, a well-known probiotic microorganism, during exposure to erythromycin, tetracycline, a ... | 2011 | 21803698 |
| probiotics for persistent diarrhea in children. | | 2011 | 21766752 |
| antimicrobial susceptibility of lactobacillus rhamnosus. | we aimed to determine the minimum inhibitory concentrations (mics) of lactobacillus rhamnosus (n=75) strains, to study their antibiotic resistance genes with microarray, and to assess the microbiological cut-off values of tested antimicrobial agents. l. rhamnosus strains were tested with agar dilution, broth microdilution and etest methods for ampicillin, clindamycin, erythromycin, gentamicin, streptomycin, and tetracycline using specific lsm medium. most of the l. rhamnosus strains were found p ... | 2010 | 21831752 |
| adaptation factors of the probiotic lactobacillus rhamnosus gg. | probiotic bacteria are administered as live micro-organisms to provide a health benefit to the host. knowledge on adaptation factors that promote the survival and persistence of probiotics in the intestine is key to understand and improve their ecological and probiotic performance. adaptation factors include adhesins, molecules conferring stress tolerance and nutritional versatility, antimicrobial products against competing microbes, and factors promoting resistance against the host immune syste ... | 2010 | 21831772 |
| lactobacilli secreting a tumor antigen and il15 activates neutrophils and dendritic cells and generates cytotoxic t lymphocytes against cancer cells. | lactobacillus rhamnosus gg (lgg) has been used to successfully induce tumor regression in an orthotopic model of bladder cancer. increased infiltration of neutrophils and macrophages into the tumor mass was observed after therapy. this study evaluates the potential of lgg to induce a directed anti-tumor response. lactobacilli were modified to secrete the prostate specific antigen (psa) or il15 and psa (il-15-psa). neutrophils and dc were exposed to lgg for 2h as in clinical therapy for bladder c ... | 2011 | 21723537 |
| short-term consumption of probiotic lactobacilli has no effect on acid production of supragingival plaque. | acidogenicity and the levels of mutans streptococci (ms) in dental plaque after the use of lactobacillus rhamnosus gg (lgg) and lactobacillus reuteri were determined. the study had a randomised, double-blind, crossover design. thirteen volunteers used tablets containing lgg or a combination of l. reuteri sd2112 and pta 5289 for 2-áweeks. at baseline and at the end of each tablet period, all available supragingival plaque was collected. lactic acid production was determined from a fixed volume (8 ... | 2011 | 21732090 |
| expression of clpl1 and clpl2 genes in lactobacillus rhamnosus vtt e-97800 after exposure to acid and heat stress treatments or to freeze-drying. | the aim of the study was to evaluate the potential of utilising the information on expression levels of selected stress genes in assessing the quality of probiotic products. for this purpose rt-qpcr methods were developed to study the expression of clpl1 and clpl2 stress genes in lactobacillus rhamnosus vtt e-97800 (e800) cells after exposure to processing-related stress conditions or to freeze-drying. heat treatments in laboratory scale were performed with e800 cells incubated at 47 -¦c or 50 - ... | 2010 | 21831761 |
| effects of synbiotic fermentation products on primary chemoprevention in human colon cells. | the consumption of synbiotics, a mixture of probiotics and indigestible food constituents such as dietary fiber, has been reported to reduce colon cancer risk. we investigated the effects of fermented wheat aleurone enriched with the probiotics lactobacillus rhamnosus gg/bifidobacterium animalis supsp. lactis on the gene expression and functional end points related to cellular defence in ht29 and primary human colon cells. aleurone was digested and fermented in vitro with/without probiotics. the ... | 2011 | 21840698 |
| characterization of certain bacterial strains for potential use as starter or probiotic cultures in dairy products. | the present work was aimed at characterizing 12 strains of lactic acid bacteria (lab) to obtain improved potential starter or probiotic cultures that could be used for making dairy products from ewe's milk and cow's milk. eight strains with antimicrobial properties, isolated from ewe's milk and from cheese made from ewe's and/or cow's milk, were studied. they were identified as enterococcus faecalis (five strains), lactococcus lactis subsp. cremoris, leuconostoc mesenteroides, and lactobacillus ... | 2011 | 21819671 |
| evaluation of beneficial attributes for phytate-degrading pediococcus pentosaceus cfr r123. | in this study we have screened and selected potent phytate-degrading lactic acid bacteria (lab), and evaluated their beneficial attributes. around 60 lab strains were isolated from several cereal- and pulse-based conventional fermented preparations. they were screened for their ability to degrade myo-inositol hexakisphosphate (ip6) by cobalt chloride qualitative staining method (plate assay). one of the cultures, pediococcus pentosaceus cfr r123, was capable of degrading both calcium and sodium ... | 2010 | 21831762 |
| anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-b1-induced liver carcinogenesis in rats. | the present investigation was carried out to evaluate the hepatoprotective effect of probiotic fermented milk (fm) containing lactobacillus rhamnosus gg and lactobacillus casei strain shirota, alone as well as in combination with chlorophyllin (chl) as an antioxidant agent in male wistar rats administered aflatoxin-b1 (afb1). afb1 was injected intraperitoneally at the rate of 450-á++g/kg body weight per animal twice a week for 6 weeks, maintaining an equal time interval between the two consecuti ... | 2011 | 21816119 |
| [effectiveness of cultured milk foods, enriched by bifidus bacteria and lactobacillus rhamnosis gg (atcc 53103, lgg) in patients with slackening of motility function of intestines]. | in patients who consumed yogurt and kefir, enriched by bifidus bacteria and lactobacillus rhamnosis gg, improvement of large intestine motor function was found. | 2011 | 21842754 |
| tocopherol and ascorbate have contrasting effects on the viability of microencapsulated lactobacillus rhamnosus gg. | the antioxidants, sodium ascorbate and tocopherol, have contrasting effects on the viability of microencapsulated lactobacillus rhamnosus gg (lgg) spray dried powders during storage (4 and 25-¦c; 32, 57 and 70% rh). the addition of tocopherol improved probiotic viability during storage while the incorporation of na-ascorbate alone or in combination with tocopherol had detrimental effects on probiotic survival. the beneficial effect of tocopherol is a consequence of its chemical antioxidative act ... | 2011 | 21842832 |
| The influence of probiotics on the cervical malignancy diagnostics quality. | Probiotics help to provide an optimum balance in the intestines. Probiotics species competitive block toxic substances and growth of unwanted bacteria and yeast species while they compete for the space and food. Lactogyn is the first oral probiotics on Serbian market dedicated to maintaining a normal vaginal flora. Lactogyn contains two well studied probiotics strains - Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14. Both of them are considered as probiotic agents with therapeutic ... | 2011 | 22191313 |
| genome sequence of lactobacillus rhamnosus strain casl, an efficient l-lactic acid producer from cheap substrate cassava. | lactobacillus rhamnosus is a type of probiotic bacteria with industrial potential for l-lactic acid production. we announce the draft genome sequence of l. rhamnosus casl (2,855,156 bp with a g+c content of 46.6%), which is an efficient producer of l-lactic acid from cheap, nonfood substrate cassava with a high production titer. | 2011 | 22123765 |
| stability of lactobacilli encapsulated in various microbial polymers. | various microbial polymers, namely xanthan gum, gellan gum, pullulan gum and jamilan, were tested as a suitable encapsulating material for lactobacillus plantarum crl 1815 and lactobacillus rhamnosus atcc 53103. resulting capsules were also studied for their ph and simulated gastrointestinal conditions tolerance. the morphology of the microcapsules was studied using scanning electron microscopy. ph tolerance was tested at ph 2.0, 3.5, 5.0 and 6.5 over a 6h incubation period. simulated gastrointe ... | 2011 | 22099374 |
| The role of copper in the manufacture of Finnish Emmental cheese. | The effects of added copper in the manufacture of Finnish Emmental cheese were studied. Consequently, cheeses were produced with or without the copper supplement and a facultative heterofermentative strain, Lactobacillus rhamnosus Lc705, which is currently utilized as a protective culture in large-scale manufacture in Finland. Cheeses were examined at 1, 7, 30, 60, and 90 d from the microbiological, chemical, and sensory points of view. Organic acid production was affected by the presence of cop ... | 2011 | 21943734 |
| Influence of mother's intestinal microbiota on gut colonization in the infant. | The aim here was to elucidate the mother-infant association in the gut colonization of 1-6 month-old infants and to establish whether probiotics can influence this process. Fecal samples from 80 mother-infant pairs were analyzed at 1 month (mothers and infants) and 6 months (infants) by real-time polymerase chain reaction to assess bacterial numbers. This double-blind placebo-controlled trial involved 2 different probiotic combinations (1. Lactobacillus rhamnosus + Bifidobacterium longum and 2. ... | 2011 | 21983067 |
| human bocavirus in the nasopharynx of otitis-prone children. | human bocavirus (hbov) is frequently identified in children with respiratory tract infections, and its role in acute otitis media (aom) has been suggested. the disease associations for the closely related bocaviruses hbov2-4 remain unknown. increasing evidence shows that probiotics may reduce the risk of aom of viral origin. objectives of the study was to examine the prevalence and persistence of bocaviruses in consecutive nasopharyngeal samples (nps) of otitis-prone children, and whether an ass ... | 2011 | 22119148 |
| Lactobacillus probiotic protects intestinal epithelium from radiation injury in a TLR-2/cyclo-oxygenase-2-dependent manner. | BackgroundThe small intestinal epithelium is highly sensitive to radiation and is a major site of injury during radiation therapy and environmental overexposure.ObjectiveTo examine probiotic bacteria as potential radioprotective agents in the intestine.Methods8-week-old C57BL/6 wild-type or knockout mice were administered probiotic by gavage for 3 days before 12 Gy whole body radiation. The intestine was evaluated for cell-positional apoptosis (6 h) and crypt survival (84 h).ResultsGavage of 5×1 ... | 2011 | 22027478 |
| flavour profiles of dry sausages fermented by selected novel meat starter cultures. | probiotic or bioprotective lactobacillus rhamnosus strains gg, lc-705 and e-97800 as well as pediococcus pentosaceus e-90390 and lactobacillus plantarum e-98098 were studied for their ability to act as main fermenting organisms in the manufacturing process of dry sausages. in the preliminary tests, their abilities to produce lactic acid and biogenic amines, histamine or tyramine, were studied in mrs broth and analysed by high-performance liquid chromatography. the strains produced higher or equa ... | 2001 | 22062105 |
| Bio-control of waterborne pathogens using Lactobacillus spp. | Bacteria play a significant role in water contamination. Chemicals are mostly used for the treatment of bacteriologically contaminated water. The use of bacterial interactions is a new approach to limit the pathogens' growth. Detection of antimicrobial substances produced by lactic acid bacteria against the waterborne pathogens is the objective of this work. Microbiological and biochemical methods were used to identify lactic acid bacteria having an antimicrobial activity. Evaluation of antimicr ... | 2011 | 22131013 |
| impact of maternal probiotic-supplemented dietary counseling during pregnancy on colostrum adiponectin concentration: a prospective, randomized, placebo-controlled study. | background: the breast milk bioactive substances such as adiponectin, have a presumably long-term impact upon the health and well-being of a child. aim: to determine the impact of probiotic-supplemented dietary counseling during pregnancy on colostrum adiponectin concentration. study design and subjects: altogether 256 pregnant women were randomized into three study groups: dietary intervention with probiotics (diet/probiotics) or with placebo (diet/placebo) and a control group (control/placebo) ... | 2011 | 21945174 |
| lactobacillus rhamnosus gg treatment potentiates intestinal hypoxia-inducible factor, promotes intestinal integrity and ameliorates alcohol-induced liver injury. | gut-derived endotoxin is a critical factor in the development and progression of alcoholic liver disease (ald). probiotics can treat alcohol-induced liver injury associated with gut leakiness and endotoxemia in animal models, as well as in human ald; however, the mechanism or mechanisms of their beneficial action are not well defined. we hypothesized that alcohol impairs the adaptive response-induced hypoxia-inducible factor (hif) and that probiotic supplementation could attenuate this impairmen ... | 2011 | 22093263 |
| the fate of indigenous microbiota, starter cultures, escherichia coli, listeria innocua and staphylococcus aureus in danish raw milk and cheeses determined by pyrosequencing and quantitative real time (qrt)-pcr. | the purpose of this work was to study the bacterial communities in raw milk and in danish raw milk cheeses using pyrosequencing of tagged amplicons of the v3 and v4 regions of the 16s rdna and cdna. furthermore, the effects of acidification and ripening starter cultures, cooking temperatures and rate of acidification on survival of added escherichia coli, listeria innocua and staphylococcus aureus in cheeses at different stages of ripening were studied by pyrosequencing and quantitative real tim ... | 2011 | 22154239 |
| the spatial distribution of bacteria in grana-cheese during ripening. | the microbial composition and its spatial distribution of grana trentino, a hard parmesan-like cheese, was determined, from vat milk to cheese. after cutting along the vertical axis of the cheese wheels, three layers were sampled diagonally across the cheese: under the cheese rind, an intermediate section and the cheese core. after two different ripening periods (9 and 18 months), the cheese samples were analysed using traditional culture dependent and culture independent methods. milk samples w ... | 2012 | 21943677 |
| Oral lactoferrin for the prevention of sepsis and necrotizing enterocolitis in preterm infants. | Lactoferrin, a normal component of human colostrum, milk, tears and saliva can enhance host defence and may be effective in the prevention of sepsis and necrotizing enterocolitis (NEC) in preterm neonates. | 2011 | 21975765 |
| effect of pepsin-treated bovine and goat caseinomacropeptide on escherichia coli and lactobacillus rhamnosus in acidic conditions. | caseinomacropeptide (cmp) is a 7-kda phosphoglycopolypeptide released from κ-casein during milk digestion and in the cheesemaking process. the objective of the study was to analyze the effect of pepsin-treated cmp from cow and goat milk on the resistance of escherichia coli and lactobacillus rhamnosus during acid stress. bacterial cells in the exponential growth phase were suspended in acidified phosphate buffered saline with or without pepsin-treated cmp. viability was determined during a 90-mi ... | 2012 | 22192178 |
| Growth phase-associated changes in the proteome and transcriptome of Lactobacillus rhamnosus GG in industrial-type whey medium. | The growth phase during which probiotic bacteria are harvested and consumed can strongly influence their performance as health-promoting agents. In this study, global transcriptomic and proteomic changes were studied in the widely used probiotic Lactobacillus rhamnosus GG during growth in industrial-type whey medium under strictly defined bioreactor conditions. The expression of 636 genes (P = 0.01) and 116 proteins (P < 0.05) changed significantly over time. Of the significantly differentially ... | 2011 | 21883975 |
| A comprehensive post-market review of studies on a probiotic product containing Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011. | The probiotic preparation Lacidofil® has been commercially available in Europe, Asia and North America since 1995. This product is a combination of two strains, Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011. The strains have been evaluated for safety, identity and mechanisms of probiotic action in vitro, in animal models and human clinical trials. The strains adhered to human epithelial cells, helped to maintain the barrier function and blocked the adhesion of a number of path ... | 2011 | 22146691 |
| Enteric commensal bacteria induce extracellular signal-regulated kinase pathway signaling via formyl peptide receptor-dependent redox modulation of dual specific phosphatase 3. | The normal microbial occupants of the mammalian intestine are crucial for maintaining gut homeostasis, yet the mechanisms by which intestinal cells perceive and respond to the microbiota are largely unknown. Intestinal epithelial contact with commensal bacteria and/or their products has been shown to activate noninflammatory signaling pathways, such as extracellular signal-related kinase (ERK), thus influencing homeostatic processes. We previously demonstrated that commensal bacteria stimulate E ... | 2011 | 21921027 |
| Bacterial DNA from orthopedic implants after routine removal. | Bacterial 16S rDNA was monitored and identified from orthopedic metallic implants after routine or septic removal from patients in a German hospital. From March to June 2009, 28 metallic implants, 10 human biopsies, and 6 foam dressings from 28 patients were investigated. After analysis of this first collective, the methods were optimized to enhance sensitivity and to reduce interference with human DNA. Then a second collective consisting of 21 metallic implants from 21 patients was investigated ... | 2011 | 22094566 |
| effects of lactobacillus rhamnosus gg supplementation on cow's milk allergy in a mouse model. | abstract: | 2011 | 22145744 |
| probiotics for prevention and treatment of diarrhea. | probiotics have been extensively studied over the past several years in the prevention and, to a larger extent, in the treatment of diarrheal diseases, especially in pediatric populations. diarrhea is a symptom, and not a disease. this review will not address chronic disorders associated with diarrhea, or clostridium difficile-induced diarrhea. rather it will focus on published clinical trials performed on acute-onset, likely infectious diarrhea occurring in the settings of day-care centers, in ... | 2011 | 21992955 |
| cdna-aflp to study the transcriptional responses of lactobacillus rhamnosus growing in cheese-like medium. | aims: lactobacillus rhamnosus is a dominant species during parmigiano reggiano cheese ripening and exhibits a great adaptability to unfavorable growth conditions. gene expression of a lact. rhamnosus, isolated from parmigiano reggiano cheese, grown in a rich medium (mrs) and in a cheese-like medium (cb) has been compared by a novel cdna amplified fragment length polymorphism (cdna-aflp) protocol. methods and results: two techniques, capillary and gel electrophoresis cdna-aflp, were applied to ... | 2011 | 21762473 |
| lactobacillus rhamnosus gg as an effective probiotic for murine giardiasis. | the gut microflora is an important constituent in the intestinal mucosal barrier and has been introduced as the concept of probiotic therapy that beneficially affects the host by improving its intestinal microbial balance. therefore, the main objective of the study was to explore the protective potential of various lactobacilli strains for murine giardiasis. by experimentation, it was found that the probiotic supplementation of either lactobacillus casei, l. acidophilus, l. plantarum, or l. rham ... | 2011 | 21760784 |
| effect of food models and low-temperature storage on the adhesion of lactobacillus rhamnosus gg to caco-2 cells. | this study evaluated the effects of fat and sugar levels on the surface properties of lactobacillus rhamnosus gg during storage in food model systems, simulating yogurt and ice cream, and related them with the ability of the bacterial cells to adhere to caco-2 cells. freeze-dried l. rhamnosus gg cells were added to the model food systems and stored for 7 days. the bacterial cells were analyzed for cell viability, hydrophobicity, ? potential, and their ability to adhere to caco-2 cells. the resul ... | 2011 | 21756003 |
| effect of acid stress on protein expression and phosphorylation in lactobacillus rhamnosus gg. | acidic environments encountered in food products and during gastrointestinal tract passage affect the survival of bacteria that are marketed as probiotics. in this study, the global proteome responses of the probiotic lactic acid bacterium lactobacillus rhamnosus gg to two physiologically relevant ph conditions (ph 4.8 and ph 5.8) were studied by 2-d dige. the proteomics data were complemented with transcriptome analyses by whole-genome dna microarrays. the cells were cultured in industrial-type ... | 2011 | 22119544 |
| deciphering the nanometer-scale organization and assembly of lactobacillus rhamnosus gg pili using atomic force microscopy. | in living cells, sophisticated functional interfaces are generated through the self-assembly of bioactive building blocks. prominent examples of such biofunctional surfaces are bacterial nanostructures referred to as pili. although these proteinaceous filaments exhibit remarkable structure and functions, their potential to design bioinspired self-assembled systems has been overlooked. here, we used atomic force microscopy (afm) to explore the supramolecular organization and self-assembly of pili ... | 2011 | 22149133 |
| ingestion of lactobacillus strain regulates emotional behavior and central gaba receptor expression in a mouse via the vagus nerve. | there is increasing, but largely indirect, evidence pointing to an effect of commensal gut microbiota on the central nervous system (cns). however, it is unknown whether lactic acid bacteria such as lactobacillus rhamnosus could have a direct effect on neurotransmitter receptors in the cns in normal, healthy animals. gaba is the main cns inhibitory neurotransmitter and is significantly involved in regulating many physiological and psychological processes. alterations in central gaba receptor exp ... | 2011 | 21876150 |
| protective effect of lactobacillus rhamnosus 231 against n-methyl-n'-nitro-n-nitrosoguanidine in animal model. | the protective effect of lactobacillus rhamnosus 231 (lr 231) against potent carcinogen n-methyl-n'-nitro-n-nitrosoguanidine (mnng) in the rat model is studied. daily feeding with lr 231 improved the body weight of male wistar rats compared to control groups. fecal azoreductase (p < 0.001) and nitroreductase (p < 0.01) enzyme activity decreased significantly in lr 231 group in comparison with control groups that received only phosphate buffer or mnng. oral administration of mnng led to a signifi ... | 2011 | 22157237 |
| lactobacillus rhamnosus administration causes sepsis in a cardiosurgical patient--is the time right to revise probiotic safety guidelines? | a 24-year-old female patient developed sepsis resulting from preoperative administration of probiotics following an aortic valve replacement. blood cultures revealed the causative agent to be the probiotic lactobacillus rhamnosus, which has recently been implicated as an emerging aetiology of infection in those taking probiotics. in the past few years, probiotic use in hospitals has increased greatly. however, there is growing global evidence that the use of probiotics in patients with organ fai ... | 2011 | 21848974 |
| probiotics can induce follicle maturational competence: the danio rerio case. | in this study, the effects of the probiotic lactobacillus rhamnosus imc501, on oocyte maturational competence acquiring was examined in zebrafish (danio rerio). l. rhamnosus administration induced the responsiveness of incompetent follicles (iiia) to 17,20-dihydroxy-4-pregnen-3-one (mih) and their in vitro maturation. the acquiring of competence of the iiia follicles was further validated by changes of lhr, mprb, inhbaa (activinbetaa1), tgfb1, and gdf9 gene expression, which have recently emerge ... | 2011 | 22088919 |
| the impact of meals on a probiotic during transit through a model of the human upper gastrointestinal tract. | commercial literature on various probiotic products suggests that they can be taken before meals, during meals or after meals or even without meals. this has led to serious confusion for the industry and the consumer. the objective of our study was to examine the impact of the time of administration with respect to mealtime and the impact of the buffering capacity of the food on the survival of probiotic microbes during gastrointestinal transit. we used an in vitro digestive system (ividis) mode ... | 2011 | 22146689 |
| Bovine Lactoferrin Prevents Invasive Fungal Infections in Very Low Birth Weight Infants: A Randomized Controlled Trial. | Background: Lactoferrin is a mammalian milk glycoprotein involved in innate immunity. Recent data show that bovine lactoferrin (bLF) prevents late-onset sepsis in preterm very low birth weight (VLBW) neonates. Methods: This is a secondary analysis of data from a multicenter randomized controlled trial where preterm VLBW neonates randomly received bLF (100 mg/day; group A1), bLF + Lactobacillus rhamnosus GG (10(6) colony-forming units per day; group A2), or placebo (group B) for 6 weeks. Here we ... | 2012 | 22184648 |
| The effect of Lactobacillus rhamnosus GG supplemented enteral feeding on the microbiotic flora of preterm infants-double blinded randomized control trial. | Intestinal flora of preterms, dominantly presents with decreased amounts of physiological microbiota. This double blinded randomized control trial compared the stool of bottle fed preterms, randomized to receive lactobacillus rhamnosus GG (LGG) 6x109or placebo with formula feeding. | 2012 | 22055271 |
| lactobacillus brantae sp. nov., isolated from feces of canada geese (branta canadensis). | three strains of lactic acid bacteria (lab) were isolated from feces of apparently healthy wild canada geese (branta canadensis) in 2010 using the cultivation of fecal lab on rogosa sl agar under aerobic conditions. these three isolates were found to share 99.9% sequence similarity of their 16s rrna, the 16s-23s intergenic transcribed spacer region (its), partial 23s rrna, rpob, rpoc, rpoa, and phes gene sequences. however, at the same time these strains exhibited lower levels of sequence simila ... | 2011 | 22021580 |
| the effect of a multispecies probiotic mixture on the symptoms and fecal microbiota in diarrhea-dominant irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. | background:: the clinical effect of probiotics on irritable bowel syndrome (ibs) is still controversial. aims:: we aimed to evaluate the effects of a probiotic mixture on ibs symptoms and the composition of fecal microbiota in patients with diarrhea-dominant ibs (d-ibs). methods:: fifty patients with d-ibs were randomized into placebo or probiotic mixture (lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus, bifidobacterium breve, bifidobacterium lactis, bifidobacterium l ... | 2011 | 22157240 |
| Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells. | Lactobacillus rhamnosus GG, a probiotic with good survival capacity in the human gut, has well-documented adhesion properties and health effects. Recently, spaCBA-encoded pili that bind to human intestinal mucus were identified on its cell surface. Here, we report on the phenotypic analysis of a spaCBA pilus knockout mutant in comparison with the wild type and other adhesin mutants. The SpaCBA pilus of L. rhamnosus GG showed to be key for efficient adherence to the Caco-2 intestinal epithelial c ... | 2012 | 22020518 |
| use of rosemary, oregano, and commercial blend of essential oils in broiler chickens: in vitro antimicrobial activities and effects on growth performance. | the present study was conducted to characterise the in vitro antimicrobial activities of 3 essential oils [oregano, rosemary, and a commercial blend of essential oils (beo)] against pathogenic and non pathogenic bacteria and to evaluate their effects on broiler chicken performances. the chemical composition of the essential oils was determined using the gas chromatography interfaced with a mass spectroscopy. the disc diffusion method, the minimum inhibitory concentration (mic), and the minimum b ... | 2011 | 22064737 |
| cell enumeration and visualisation by transmission electron microscopy of lactobacillus rhamnosus treated with cinnamon (cinnamomum zeylanicum b.) essential oil. | the use of essential oils (eos) in functional foods containing probiotic microorganisms must consider the antimicrobial activity of these oils against beneficial bacteria such as lactobacillus rhamnosus. this study aimed to evaluate the sensitivity of l. rhamnosus cultures treated with cinnamon eo through viable cell counts and visualisation by transmission electron microscopy. cinnamon eo at a concentration of 0.04% had a bacteriostatic activity after 2 h of incubation. although slight alterati ... | 2011 | 21999302 |
| assessment of the fecal lactobacilli population in patients with hepatitis b virus-related decompensated cirrhosis and hepatitis b cirrhosis treated with liver transplant. | this study aims to provide an overview of the diversity of intestinal lactobacillus among chinese patients with hepatitis b virus (hbv)-related decompensated cirrhosis and who received liver transplant for hepatitis b cirrhosis. fecal samples were collected from 38 healthy volunteers, 61 patients with hbv-related decompensated cirrhosis (group lc) and 74 patients who had liver transplant for hepatitis b cirrhosis (group lt). quantitative polymerase chain reaction technology with species-specific ... | 2011 | 21965156 |
| Development of a locally sustainable functional food for people living with HIV in Sub-Saharan Africa: laboratory testing and sensory evaluation. | The use of Lactobacillus rhamnosus GR-1 and micronutrients has been associated with a preserved immune function among people living with HIV. However, use of these products in the developing world remains limited due to the lack of facilities for production. We describe the development of a yogurt with L. rhamnosus GR-1 at >7×10(7) colony forming units fortified with locally grown Moringa oleifera leaves at 20% of the recommended daily allowance of vitamin A. The product was made by preparing a ... | 2011 | 21986358 |
| Streptococci-Human Papilloma Virus Interaction With Ethanol Exposure Leads to Keratinocyte Damage. | PURPOSE: Ethanol, human papilloma virus (HPV), and poor oral hygiene are risk factors that have been attributed to oral carcinogenesis. Streptococci sp and HPV infections are common in the head and neck, often associated with sexual activity. Although HPV is linked to head and neck squamous cell carcinoma, it is unclear whether there is a similar role for Streptococci sp. This cell study examines whether Streptococci sp and HPV-16 with exposure to ethyl alcohol (ETOH) can act as cofactors in the ... | 2011 | 22079067 |
| The impact of perinatal probiotic intervention on gut microbiota: Double-blind placebo-controlled trials in Finland and Germany. | Specific probiotic combinations during early feeding, via the mother or incorporated in early formula-feeding, mold the intestinal microbiota composition in infants. The objective was to analyze the impact of probiotic administration to mother or infant on gut microbiota composition in 6-month-old Finnish and German infants. In Finland probiotics were given to mothers (n = 79) for 2 months prior to and 2 months after delivery. In Germany probiotics were started in infants (n = 81) at weaning, at ... | 2011 | 21979491 |
| Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus. | The purpose of this study was to develop a mucoadhesive coacervate microparticulate system to deliver viable Lactobacillus rhamnosus cells into the gut for an extended period of time while maintaining high numbers of viable cells within the formulation throughout its shelf-life and during gastrointestinal transit. | 2011 | 21984867 |
| prenatal administration of lactobacillus rhamnosus has no effect on the diversity of the early infant gut microbiota. | to cite this article: ismail ih, oppedisano f, joseph sj, boyle rj, robins-browne rm, tang mlk. prenatal administration of lactobacillus rhamnosus has no effect on the diversity of the early infant gut microbiota. pediatric allergy immunology 2011: doi: 10.1111/j.1399-3038.2011.01239.x abstract: we have recently shown that maternal administration of lactobacillus rhamnosus gg (lgg) during late pregnancy can have beneficial effects on the early development of infant gut microbiota, promoting a bi ... | 2011 | 22136660 |
| structural studies of the exopolysaccharide consisting of a nonasaccharide repeating unit isolated from lactobacillus rhamnosus kl37b. | a novel structure of exopolysaccharide from the lactic acid bacteria (lab) lactobacillus rhamnosus kl37b, from the human intestinal flora, is described. during the structural investigation of the exopolysaccharide it was found that the repeating unit is a nonasaccharide, which is the largest repeating unit found in lab exopolysaccharides to date. the polysaccharide material was prepared by tca extraction of a bacterial cell mass, purified by anion-exchange and gel permeation chromatography and c ... | 2011 | 22063501 |
| probiotics for the prevention of pediatric antibiotic-associated diarrhea. | antibiotics alter the microbial balance within the gastrointestinal tract. probiotics may prevent antibiotic-associated diarrhea (aad) via restoration of the gut microflora. antibiotics are prescribed frequently in children and aad is common in this population. | 2011 | 22071814 |
| Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. | To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We asse ... | 2012 | 21989255 |
| Randomised clinical trial: the synbiotic food supplement Probiotical vs. placebo for acute gastroenteritis in children. | Some probiotic strains reduce the duration of acute diarrhoea. As a result of strain and product specificity, each product needs support by clinical data. | 2011 | 21899583 |
| Treatment with probiotics in experimental oral colonization by Candida albicans in murine model (DBA/2). | Oral Diseases (2011) The aim of this study is to evaluate the oral colonization by Candida albicans in experimental murine immunosuppressed DBA/2 and treatment with probiotic bacteria. To achieve these objectives, 152 DBA/2-immunosuppressed mice were orally inoculated with a suspension of C. albicans containing 10(8) viable yeast cells, the animals were treated with nystatin or with the probiotics (Lactobacillus acidophilus and Lactobacillus rhamnosus). Evaluations were performed by Candida cou ... | 2011 | 22059932 |
| Immunoregulatory potential of exopolysaccharide from Lactobacillus rhamnosus KL37. Effects on the production of inflammatory mediators by mouse macrophages. | The ability to produce exopolysaccharides (EPS) is widespread among lactobacilli including Lactobacillus rhamnosus, the commonly used probiotic bacteria. Exopolysaccharides are a major component of the bacterial biofilm with a well-documented impact on adherence of bacteria to host cells. However, their immunoregulatory properties are unknown. The aim of this study was to examine the immunostimulatory potential of EPS derived from L. rhamnosus KL37. We investigated the effect of EPS on the produ ... | 2011 | 21950581 |
| Probiotic Bacteria Induce Maturation of Intestinal Claudin 3 Expression and Barrier Function. | An immature intestinal epithelial barrier may predispose infants and children to many intestinal inflammatory diseases, such as infectious enteritis, inflammatory bowel disease, and necrotizing enterocolitis. Understanding the factors that regulate gut barrier maturation may yield insight into strategies to prevent these intestinal diseases. The claudin family of tight junction proteins plays an important role in regulating epithelial paracellular permeability. Previous reports demonstrate that ... | 2011 | 22155109 |
| Probiotic yogurt consumption may improve gastrointestinal symptoms, productivity, and nutritional intake of people living with human immunodeficiency virus in Mwanza, Tanzania. | The gut-associated lymphoid tissue is a major site of human immunodeficiency virus (HIV) activity and significantly influences disease prognosis. Reducing immune activation due to gastroenteritis may thus help slow disease progression. Probiotic microorganisms have considerable immunomodulatory effects at the level of the gut-associated lymphoid tissue. A probiotic yogurt initiative was thus established in Mwanza, Tanzania, to improve gastrointestinal (GI) integrity and reduce the incidence and ... | 2011 | 22153512 |
| meta-analysis: the effects of lactobacillus rhamnosus gg supplementation for the prevention of healthcare-associated diarrhoea in children. | in children, healthcare-associated diarrhoea, in particular, due to rotavirus, may prolong the hospital stay and increase medical costs, prompting interest in effective, low-cost, preventive strategies. | 2011 | 21899584 |
| Probiotic properties of Weissella strains isolated from human faeces. | Three Weissellaconfusa and five Weissellacibaria strains were previously isolated from human faeces and their potential as probiotics was examined in this work. Resistance to low pHs (pH 2.0 and 3.0) and 0.3% bile salt were examined. Enzyme activities, susceptibilities to heat treatment and various antibiotics, and adhesion capacities to Caco-2 cells were also examined. All Weissella strains were killed when exposed to pH 2.0 for 2 h but survived at pH 3.0 with different survival ratios. W. conf ... | 2011 | 22200451 |
| successful tigecycline lock therapy in a lactobacillus rhamnosus catheter-related bloodstream infection. | introduction: catheter-related bloodstream infections very often involve the premature removal of long-term intravascular devices (ltid). the antibiotic lock therapy (alt) represents a conservative approach to the treatment of uncomplicated infections of tunneled ltid when catheter removal is not a feasible option. case report: we present here the first reported case of tunneled ltid bloodstream infection due to ... | 2011 | 22005933 |
| In vitro testing of commercial and potential probiotic lactic acid bacteria. | Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to investigate the diversity of selected commercial and potential probiotic lactic acid bacteria using common in vitro screening assays such as transit tolerance in the upper human gastrointestinal tract, adhesion capacity to human intestinal cell lines and effect on epithelial barrier function. The selected bacteria include strains of Lac ... | 2011 | 22177712 |
| Peptidoglycan recognition protein 3 (PglyRP3) has an anti-inflammatory role in intestinal epithelial cells. | Intestinal epithelial cells produce cytokines in response to bacterial peptidoglycan (PGN), which is detected by several classes of pattern-recognition receptors (PRRs) as peptidoglycan recognition proteins (PGlyRPs), Toll-like receptor 2 (TLR2) and NOD receptors. All types of PGlyRPs recognize bacterial peptidoglycan and function in antibacterial innate immunity. In this study, we investigated the role of PGlyRP3 in the response of intestinal epithelial cells (Caco-2) to PGN from pathogenic (St ... | 2011 | 22099350 |
| microchip electrophoresis of bacteria using lipid-based liquid crystalline nanoparticles. | the aggregation and adhesion of bacterial cells is a serious disadvantage for electrophoretic separations of bacteria. in this study, lipid-based liquid crystalline nanoparticles were used as a pseudostationary phase to minimise the bacterial aggregation and adsorption to the inner walls of microchannels. lactobacillus delbrueckii subsp. bulgaricus, streptococcus thermophilus and lactobacillus rhamnosus were selected as analytes and were separated by microchip electrophoresis (mce) with laser-in ... | 2011 | 21968347 |
| using the good to beat out the bad: probiotics for eliminating vancomycin-resistant enterococci colonization. | | 2011 | 21989278 |
| lactobacillus rhamnosus gg and bifidobacterium animalis mb5 induce intestinal but not systemic antigen-specific hyporesponsiveness in ovalbumin-immunized rats. | probiotics may modulate the host immune response by mechanisms not yet fully understood. we evaluated the modulation of intestinal and systemic antigen-specific immune response by lactobacillus rhamnosus gg (lgg) or bifidobacterium animalis mb5 in tolerized and immunized rats. three groups of rats received orally lgg, b. animalis, or pbs (control) for 28 d. each group was divided into two subgroups of tolerized or immunized rats receiving orally ovalbumin (ova; 7 mg) or pbs on d 7, 9, and 11. al ... | 2012 | 22223570 |
| effect of micronutrient and probiotic fortified yogurt on immune-function of anti-retroviral therapy naive hiv patients. | background: micronutrient supplementation has been shown to reduce the progression of hiv but does not have an effect on the intestinal barrier or the intestinal microbiota of hiv patients. studies have suggested that probiotics could potentially complement micronutrients in preserving the immune-function of hiv patients. objective: assess the impact of micronutrient supplemented probiotic yogurt on the immune function of hiv patients. design:we performed a randomized, double blind, controlled t ... | 2011 | 22254084 |
| genome sequence of lactobacillus rhamnosus atcc 8530. | lactobacillus rhamnosus is found in the human gastrointestinal tract and is important for probiotics. we became interested in l. rhamnosus isolate atcc 8530 in relation to beer spoilage and hops resistance. we report here the genome sequence of this isolate, along with a brief comparison to other available l. rhamnosus genome sequences. | 2012 | 22247527 |
| lactobacillus rhamnosus gr-1 enhances nf-kappab activation in escherichia coli-stimulated urinary bladder cells through tlr4. | abstract: background: epithelial cells of the urinary tract recognize pathogenic bacteria through pattern recognition receptors on their surface, such as toll-like receptors (tlrs), and mount an immune response through the activation of the nf-kappab pathway. some uropathogenic bacteria can subvert these cellular responses, creating problems with how the host eliminates pathogens. lactobacillus is a genus of lactic acid bacteria that are part of the microbiota and consist of many probiotic stra ... | 2012 | 22264349 |
| the antimicrobial properties of the lowbush blueberry (vaccinium angustifolium) fractional components against foodborne pathogens and the conservation of probiotic lactobacillus rhamnosus. | the antimicrobial properties of lowbush blueberry (vaccinium angustifolium) were studied against escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and lactobacillus rhamnosus to determine which fractional components have antimicrobial effects and which microorganisms are most susceptible to these antimicrobial properties. lowbush blueberry extract (f1) was separated using a c-18 sep-pak cartridge into monomeric phenolics (f2) and anthocyanins plus proanthocyanidins (f3). ... | 2012 | 22265292 |
| dietary addition of lactobacillus rhamnosus gg impairs the health of escherichia coli f4-challenged piglets. | lactobacillus rhamnosus gg (lgg) is a probiotic for humans and is normally not found in pigs; however, it has been shown to protect the human-derived intestinal caco-2 cells against the damage induced by an important intestinal pathogen, enterotoxigenic escherichia coli f4 (etec). an experiment was conducted to test whether the dietary addition of lgg improves the growth and health of weaned pigs when orally challenged by e. coli f4. thirty-six pigs were weaned at 21 days and assigned to a stand ... | 2011 | 22440280 |
| antimicrobial and safety properties of lactobacilli isolated from two cameroonian traditional fermented foods. | twenty-one lactobacillus isolates from "sha'a" (a maize - based fermented beverage) and "kossam" (traditionally fermented cow milk) were selected in accordance with their antagonistic activities and tested for their bacteriocinogenic potential as well as safety properties. these isolates were preliminarily identified as lactobacillus plantarum (62%), lactobacillus rhamnosus (24%), lactobacillus fermentum (10%) and lactobacillus coprophilus (4%) based on phenotypic characteristics and rep-pcr gen ... | 2011 | 22396914 |
| effects of chlorhexidine, listerine and fluoride listerine mouthrinses on four putative root-caries pathogens in the biofilm. | to investigate the effects of chlorhexidine (chx), listerine and fluoride listerine on putative root-caries pathogens in the biofilm in the artificial mouth model. | 2011 | 22319755 |
| acid production and growth by oral lactobacillus species in vitro. | to analyze the acid-producing and growth abilities of different oral lactobacillus species. | 2011 | 22298522 |
| the effect of rice fibre fractions on the growth of co-cultures of probiotics. | the fermentation of rice dietary fibre was measured by the cell yield, ph, optical density specific growth rate and biomass using nine co-cultures of four probiotics at 0, 6, 24 and 48 h incubation. the results from the fermentation of the soluble, insoluble and total dietary fibre (sdf, idf, and tdf) of two rice varieties were compared. overall there was no significant difference (p < 0.05) in the fermentation of the six different rice fibre fractions. however, co-cultures showed a preference f ... | 2011 | 23572712 |
| a comprehensive approach to determine the probiotic potential of human-derived lactobacillus for industrial use. | specific strains should only be regarded as probiotics if they fulfill certain safety, technological and functional criteria. the aim of this work was to study, from a comprehensive point of view (in vitro and in vivo tests), three lactobacillus strains (lactobacillus paracasei jp1, lactobacillus rhamnosus 64 and lactobacillus gasseri 37) isolated from feces of local newborns, determining some parameters of technological, biological and functional relevance. all strains were able to adequately g ... | 2012 | 23498174 |
| [effectiveness and safety of probiotics in children on the basis of clinical trials]. | probiotics may be helpful if they are used according to current medical knowledge based on the results of properly designed and well-conducted clinical trials. their effects depend on the specific strain and defined dose of the probiotic microorganism, and should not be extrapolated on other, even closely related strains. meta-analyses of randomized clinical trials performed in children showed the greatest probiotic efficacy of lactobacillus rhamnosus gg (lgg) and saccharomyces boulardii strains ... | 2012 | 23378402 |
| evaluating the effect of probiotic containing milk on salivary mutans streptococci levels. | to evaluate the changes in mutans streptococci counts in saliva after short term probiotic intervention and its delayed effects on salivary mutans streptococci count. | 2012 | 23342560 |
| anti-inflammatory lactobacillus rhamnosus cncm i-3690 strain protects against oxidative stress and increases lifespan in caenorhabditis elegans. | numerous studies have shown that resistance to oxidative stress is crucial to stay healthy and to reduce the adverse effects of aging. accordingly, nutritional interventions using antioxidant food-grade compounds or food products are currently an interesting option to help improve health and quality of life in the elderly. live lactic acid bacteria (lab) administered in food, such as probiotics, may be good antioxidant candidates. nevertheless, information about lab-induced oxidative stress prot ... | 2012 | 23300685 |