Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| quantitative strain-specific detection of lactobacillus rhamnosus gg in human faecal samples by real-time pcr. | to develop a strain-specific rapid assay for identification and quantification of lactobacillus rhamnosus gg in human faecal samples. | 2009 | 19200317 |
| modeling and experimental studies on intermittent starch feeding and citrate addition in simultaneous saccharification and fermentation of starch to flavor compounds. | simultaneous saccharification and fermentation (ssf) is a combined process of saccharification of a renewable bioresource and fermentation process to produce products, such as lactic acid and ethanol. recently, ssf has been extensively used to convert various sources of cellulose and starch into fermentative products. here, we present a study on production of buttery flavors, namely diacetyl and acetoin, by growing lactobacillus rhamnosus on a starch medium containing the enzyme glucoamylase. we ... | 2009 | 19137337 |
| lactobacillus rhamnosus gg and lactobacillus casei suppress escherichia coli-induced chemokine expression in intestinal epithelial cells. | recently, some strains of lactic acid bacteria (lab) have been reported to prevent the development of atopic dermatitis and to improve allergic symptoms, especially in young children. however, the mechanisms involved in these effects are not fully understood. intestinal microbiota play critical roles in the development of host immune development and are recognized and regulated by the host through intestinal epithelial cells (iecs). we thus hypothesized that lab influence the host immune system ... | 2009 | 18716403 |
| crude goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus: contribution to proteolysis and ace inhibitory activity. | unsupplemented acid goat whey containing 0.96% protein and 2.76% lactose was fermented aerobically with 32 microflora extracted from various raw milk cheeses and dairy products. these microflora were screened for their ability to hydrolyse whey proteins (alpha-lactalbumin and/or beta-lactoglobulin) and to generate peptides inhibitors of angiotensin i converting enzyme. five microflora were able to degrade whey protein. the most efficient microflora was able to fully hydrolyse alpha-lactalbumin a ... | 2009 | 19121243 |
| spray bacteriotherapy decreases middle ear fluid in children with secretory otitis media. | secretory otitis media (som) is characterised by persistent fluid in the middle ear cavity, but the cause is unknown. we investigated the clinical, bacteriological and immunological effects of treatment with probiotic bacteria on som. | 2009 | 18713796 |
| bifidobacterium and lactobacillus dna in the human placenta. | aims: bifidobacteria and lactobacilli are part of the human normal intestinal microbiota and may possibly be transferred to the placenta. it was hypothesized that intestinal bacteria or their components are present in the placenta and that the foetus may be exposed to them. we investigated the presence of bifidobacteria and lactobacilli and their dna in the human placenta. methods and results: we studied 34 human placentae (25 vaginal and nine caesarean deliveries) for the presence bifidobacteri ... | 2009 | 19018955 |
| probiotics for the treatment or prevention of eczema. | 2009 | 18804270 | |
| sudan azo dyes and para red degradation by prevalent bacteria of the human gastrointestinal tract. | sudan azo dyes have genotoxic effects and ingestion of food products contaminated with sudan i, ii, iii, iv, and para red could lead to exposure in the human gastrointestinal tract. in this study, we examined thirty-five prevalent species of human intestinal bacteria to evaluate their capacity to degrade sudan dyes and para red. among these tested bacterial strains, 23, 13, 33, 30, and 29 out of 35 species tested were able to reduce sudan i, ii, iii, iv, and para red, respectively, to some exten ... | 2010 | 19580882 |
| the impact of perinatal probiotic intervention on the development of overweight and obesity: follow-up study from birth to 10 years. | the achievements in combating the increasing trend of overweight and obesity have thus far been inadequate. the recently discovered instrumental role of the gut microbiota in host metabolism may offer a novel target in the prevention and management of obesity. | 2010 | 20231842 |
| probiotics and immunosenescence: cheese as a carrier. | oral intake of specific probiotics has been reported to enhance the immunity of the elderly. earlier studies have used milk or yoghurt as a probiotic carrier. we chose a commercial probiotic cheese to evaluate its potential as a probiotic food. thirty-one healthy elderly volunteers (21 female, 10 male) aged from 72 to 103 (median 86) consumed a commercial probiotic cheese containing approximately 10(9) cfu day(-1) of lactobacillus rhamnosus hn001 and lactobacillus acidophilus ncfm. the 4-week pr ... | 2010 | 20236323 |
| [establishment and evaluation of the in vitro dynamic biofilm model]. | to establish a dynamic model which could simulate the ecologic environment of oral cavity and study the cariogenicity of oral biofilm, continuously observe the formation of oral biofilm. | 2010 | 20300696 |
| differentiation of three lactobacillus rhamnosus strains (e/n, oxy and pen) by sds-page and two-dimensional electrophoresis of surface-associated proteins. | sds-page of extracted surface-associated proteins of lactobacillus rhamnosus strains e/n, oxy, and pen, was performed. the obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used rapd genotypic method. the differentiation by the sds-page method proved to be a useful tool for strain-specific identification, which was further confirmed by 2de analysis. therefore, it can be used as an alternative or complementary method for both con ... | 2010 | 20372027 |
| the effects of lactobacillus rhamnosus on the prevention of asthma in a murine model. | lactobacilli are probiotic bacteria that are effective in the management of allergic diseases or gastroenteritis. it is hypothesized that such probiotics have immunoregulatory properties and promote mucosal tolerance. our goal was to investigate whether lactobacillus casei rhamnosus lcr35 could inhibit airway inflammation in an ovalbumin (ova)-induced murine model of asthma. | 2010 | 20592920 |
| porcine small intestinal epithelial cell line (ipec-j2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics. | previous studies of epithelial immune responses to rotavirus infection have been conducted in transformed cell lines. in this study, we evaluated a non-transformed porcine jejunum epithelial cell line (ipec-j2) as an in-vitro model of rotavirus infection and probiotic treatment. cell-culture-adapted porcine rotavirus (prv) osu strain, or human rotavirus (hrv) wa strain, along with lactobacillus acidophilus (la) or lactobacillus rhamnosus gg (lgg) were used to inoculate ipec-j2 cells. la or lgg t ... | 2010 | 20373994 |
| gateways to clinical trials. | (-)-epigallocatechin gallate, abafungin, ace-031, adapalene/benzoyl peroxide, ae-37, aflibercept, ags-003, albiglutide, alemtuzumab, aliskiren fumarate, alt-801, an-2728, anacetrapib, api, aprepitant, arq-197, ascorbic acid, atazanavir sulfate, atn-224, avi-4658, azacitidine, azelnidipine; belinostat, bevacizumab, bi-2536, biphasic insulin aspart, bortezomib, bovine lactoferrin, bryostatin 1, budesonide/formoterol fumarate; cac10, canfosfamide hydrochloride, cediranib, clofarabine, cocaine conju ... | 2010 | 20383346 |
| m-rtlv agar, a novel selective medium to distinguish lactobacillus casei and lactobacillus paracasei from lactobacillus rhamnosus. | we developed a novel selective medium, modified-rhamnose-2,3,5-triphenyltetrazolium chloride-lbs-vancomycin agar (m-rtlv agar), that utilizes the fermentability of l-rhamnose to distinguish lactobacillus casei and lactobacillus paracasei from lactobacillus rhamnosus. whereas l. casei and l. paracasei formed red colonies on the m-rtlv agar, l. rhamnosus formed either pink-toned colonies or white colonies with a red spot. an intervention study was conducted to confirm the capability of m-rtlv agar ... | 2010 | 20385416 |
| lactobacillus rhamnosus gg influences polyamine metabolism in hgc-27 gastric cancer cell line: a strategy toward nutritional approach to chemoprevention of gastric cance. | chemoprevention by dietary constituents has recently emerged as a novel approach to control gastric cancer incidence. over the past years, functional foods and food supplements, especially probiotics, have received much attention as potential dietary cancer prevention agents. the precise mechanisms by which these lactic cultures exert their antitumorigenic activities are not fully elucidated, but there is some evidence of their influence on cell proliferation and growth. ornithine decarboxylase ... | 2010 | 20388096 |
| administration of a synbiotic to free-living elderly and evaluation of serum cytokines. a pilot study. | ten free-living elderly were administered with a synbiotic [fermented milk containing lactobacillus rhamnosus gorbach and goldin (lgg)] and oligofructose as a prebiotic for one month. serum cytokines were evaluated before (t(0)) and after (t(1)) synbiotic administration. at t(0), values of interleukin (il)-12, il-6, il-10, il-1beta and tumor necrosis factor (tnf)-alpha were lower than normal controls, with the exception of il-8, thus confirming previous results on the impairment of both innate a ... | 2010 | 20388097 |
| improvement of l: -lactic acid production under glucose feedback controlled culture by lactobacillus rhamnosus. | in this study, a glucose feedback controller had been designed. under the controller, the glucose concentration in l: -lactic acid culture could be automatically controlled at 15 +/- 1 g/l. furthermore, l: -lactic acid production by lactobacillus rhamnosus la-04-1 in fed-batch culture was significantly increased to 170 g/l l: -lactic acid using the controller compared with 130 g/l l: -lactic acid in pulse fed-batch and 135 g/l in l: -lactic acid constant feed rate fed-batch cultures. the results ... | 2010 | 20393886 |
| immunomodulatory activity of lactobacillus rhamnosus strains isolated from goat milk: impact on intestinal and respiratory infections. | the immune stimulation induced by lactobacillus rhamnosus crl1505 (lr05) and l. rhamnosus crl1506 (lr06) on the resistance to infection with an intestinal pathogen (salmonella typhimurium) and a respiratory pathogen (streptococcus pneumoniae) was studied in swiss-albine mice experimental models. the cytokine profiles that induced the innate and specific immune response in both infectious processes were investigated. both strains were able to improve resistance against the intestinal pathogen. on ... | 2010 | 20395002 |
| screening and selection of stress resistant lactobacillus spp. isolated from the marine oyster (crassostrea gigas). | we attempted to isolate lactobacillus spp. from the marine oyster (crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. a total of 83 lactobacilli strains were isolated from oyster. they were all gram-positive, rod-shaped and catalase-negative. by performing a stress resistance assay, we selected eighteen isolates. based on 16s rrna gene sequencing, lactobacillus paracasei was the most prevalent species among the selected isolates. ... | 2010 | 20633689 |
| lactobacillus gg in the prevention of nosocomial gastrointestinal and respiratory tract infections. | the incidence of nosocomial infections, predominantly gastrointestinal and respiratory, in children in developed countries is high, ranging from 5% to 44%. there is no effective strategy for preventing these infections. the objective of our study was to investigate the role of lactobacillus gg (lgg) in preventing nosocomial gastrointestinal and respiratory tract infections at a pediatric hospital. | 2010 | 20403940 |
| efficacy of vaginal probiotic capsules for recurrent bacterial vaginosis: a double-blind, randomized, placebo-controlled study. | we assessed the effectiveness of vaginal probiotic capsules for recurrent bacterial vaginosis (bv) prevention. | 2010 | 20659602 |
| probiotics and intestinal colonization by vancomycin-resistant enterococci in mice and humans. | we investigated the impact of probiotics on the intestinal carriage of vancomycin-resistant enterococci (vre). administration of lactobacillus rhamnosus lcr35 but not escherichia coli nissle reduced, although not significantly, the density of vre colonization in a murine model. no effect of lcr35 was observed in a double-blind placebo randomized study, involving nine patients. | 2010 | 20421444 |
| [possible interaction between probiotics and warfarin]. | 2010 | 20432867 | |
| tipsitis: incidence and outcome-a single centre experience. | infection of transjugular intrahepatic portosystemic stent shunt (tipss) called 'tipsitis' has been reported but appears unusual. we report here our experience of patients who were diagnosed to have tipsitis at our centre. | 2010 | 20440117 |
| evaluation of quantitative pcr measurement of bacterial colonization of epithelial cells. | microbial colonization is an important step in establishing pathogenic or probiotic relations to host cells and in biofilm formation on industrial or medical devices. the aim of this work was to verify the applicability of quantitative pcr (real-time pcr) to measure bacterial colonization of epithelial cells. salmonella enterica and caco-2 intestinal epithelial cell line was used as a model. to verify sensitivity of the assay a competition of the pathogen cells to probiotic microorganism was tes ... | 2010 | 20734752 |
| protective effect of lactobacillus casei strain shirota against lethal infection with multi-drug resistant salmonella enterica serovar typhimurium dt104 in mice. | the anti-infectious activity of lactobacilli against multi-drug resistant salmonella enterica serovar typhimurium dt104 (dt104) was examined in a murine model of an opportunistic antibiotic-induced infection. | 2010 | 21059159 |
| optimization of medium composition for enhancing growth of lactobacillus rhamnosus pen using response surface methodology. | response surface methodology was used to optimize media components such as carbon and nitrogen (simple and complex) sources, mineral agents and growth factors (b vitamins, amino acids) for enhancing the biomass production of lactobacillus rhamnosus pen. for screening experiment the following carbon sources were selected: glucose, glucose+pyruvate, glucose+citrate, glucose+lactate, galactose, fructose, lactose, sucrose, maltose, lactulose, fructooligosaccharides, maltodextrins dp 4-7 and dp 13-17 ... | 2010 | 20734756 |
| probiotics in the treatment of acute rotavirus diarrhoea. a randomized, double-blind, controlled trial using two different probiotic preparations in bolivian children. | evidence suggests that probiotics reduce rotavirus diarrhoea duration. although there are several probiotic strains potentially useful, daily practice is often limited by the type and number of products locally available. in general, information about combined products is scarce. in this study we compare the effect of two probiotic products in the treatment of diarrhoea in children less than 2 years of age. | 2010 | 20735858 |
| preservation of viability and antibacterial activity of lactobacillus spp. in calcium alginate beads. | the objective of the study was to produce calcium alginate beads able to deliver lactobacillus spp. (lactobacillus plantarum, lactobacillus rhamnosus gg, lactobacillus bulgaricus and lactobacillus lactis) with preserved viability and antibacterial activity. four types of beads, containing entrapped (e), surface and entrapped (es), surface (s) and concentrated surface and entrapped lactobacilli (c(es)) were prepared and physically characterized. the antibacterial activity of lactobacilli cultures ... | 2010 | 20800681 |
| the determination of viable counts in probiotic cultures microencapsulated by spray-coating. | an assessment of various methods to determine viable counts (cfu) in freeze-dried and dried microencapsulated (me) probiotic cultures was carried out. microencapsulation was done by spray-coating of dried lactobacillus rhamnosus r0011 or bifidobacterium longum atcc 15708 cultures with fat. rehydration of the me powders was incomplete when they were added to water and gently agitated. as a result analytical methods based on vortexing of rehydrated me cultures and which did not incorporate a high- ... | 2010 | 20832691 |
| survival of lactobacillus rhamnosus strains in the upper gastrointestinal tract. | in the present study six probiotic lactobacillus rhamnosus strains were investigated for their ability to survive in the human upper gastrointestinal tract through a dynamic gastric model of digestion. mrs broth was used as delivery vehicle and survival was investigated during in vitro gastric and gastric plus duodenal digestion. results highlighted that all tested strains showed good survival rate during both gastric and duodenal digestion. in particular, three strains exhibited a great surviva ... | 2010 | 20832693 |
| evaluation of genetic polymorphism among lactobacillus rhamnosus non-starter parmigiano reggiano cheese strains. | parmigiano reggiano (pr) is an italian cooked, long-ripened cheese made with unheated cow's milk and natural whey starter. the microflora is involved in the manufacturing of this cheese, arising from the natural whey starter, the raw milk and the environment. molecular studies have shown that mesophilic non-starter lactic acid bacteria (nslab) are the dominant microflora present during the ripening of pr. in this study, a characterisation of lactobacillus rhamnosus isolated from a single pr manu ... | 2010 | 21131087 |
| increase of fecundity by probiotic administration in zebrafish (danio rerio). | it is well known established that reproduction is sensitive to the state of energy reserves, and that there is a balance between energy homeostasis and fertility. in this view, this study examined the effects of the probiotic lactobacillus rhamnosus, as a feed additive, on zebrafish (danio rerio) fecundity. ten days of probiotic treatment modulate the gene expression of neuropeptide hormones and metabolic signals, such as kiss1, kiss2 and leptin both at the cns level and at the peripheral level. ... | 2010 | 20833753 |
| lactobacillus rhamnosus meningitis following recurrent episodes of bacteremia in a child undergoing allogeneic hematopoietic stem cell transplantation. | we report a case of meningitis due to lactobacillus rhamnosus in a child undergoing allogeneic hematopoietic stem cell transplantation for acute leukemia. four episodes of bacteremia involving strains with pulsotypes identical to that of the cerebrospinal fluid isolate preceded meningitis. after several courses of clindamycin, no relapse occurred during the patient follow-up. | 2010 | 20844225 |
| effect of a multispecies probiotic supplement on quantity of irritable bowel syndrome-related intestinal microbial phylotypes. | probiotics can alleviate the symptoms of irritable bowel syndrome (ibs), possibly by stabilizing the intestinal microbiota. our aim was to determine whether ibs-associated bacterial alterations were reduced during multispecies probiotic intervention consisting of lactobacillus rhamnosus gg, l. rhamnosus lc705, propionibacterium freudenreichii ssp. shermanii js and bifidobacterium breve bb99. the intervention has previously been shown to successfully alleviate gastrointestinal symptoms of ibs. | 2010 | 20849659 |
| microbiological profiles in saliva and supragingival plaque from caries-active adolescents before and after a short-term daily intake of milk supplemented with probiotic bacteria - a pilot study. | the aim of the present pilot study was to investigate the microbial profile in saliva and supragingival plaque samples collected from caries-active adolescents before and after a daily short-term intake of milk supplemented with the probiotic bacteria. | 2010 | 21180676 |
| production of functional probiotic, prebiotic, and synbiotic ice creams. | in this work, 3 types of ice cream were produced: a probiotic ice cream produced by adding potentially probiotic microorganisms such as lactobacillus casei and lactobacillus rhamnosus; a prebiotic ice cream produced by adding inulin, a prebiotic substrate; and a synbiotic ice cream produced by adding probiotic microorganisms and inulin in combination. in addition to microbial counts, ph, acidity, and physical and functional properties of the ice creams were evaluated. the experimental ice creams ... | 2010 | 20854989 |
| lactobacillus rhamnosus gr-1-induced il-10 production in human placental trophoblast cells involves activation of jak/stat and mapk pathways. | intrauterine infection/inflammation complicates 25% to 40% of preterm births (ptb). the human vagina is normally populated by lactobacillus species, some of which upregulate interleukin 10 (il-10) output in lipopolysaccharide (lps)-treated human placental trophoblast cells. we hypothesize that a probiotic strain, l rhamnosus gr-1 exerts its anti-inflammatory effect through activation of the janus kinases/signal transducers and activators of transcription (jak/stat) and mitogen-activated protein ... | 2010 | 20858906 |
| development and pilot evaluation of a novel probiotic mixture for the management of seasonal allergic rhinitis. | microbial exposure may direct the immune system away from allergic-type responses, but until now probiotic interventions have had limited success in the prevention and treatment of allergic diseases. in this study, a novel probiotic mixture was specifically created based on preliminary in vitro investigations on pollen-induced immune responses. a mixture with lactobacillus rhamnosus gr-1 and a novel fecal bifidobacterium adolescentis isolate was formulated into a yogurt and tested for its effect ... | 2010 | 20921983 |
| the effects of probiotics supplementation timing on an ovalbumin-sensitized rat model. | the aim of the present study was to investigate the effect of oral probiotic bacteria administration at different times on ovalbumin-sensitized rats. brown-norway (bn) rats were orally sensitized with ovalbumin for 6 weeks. probiotics were administered before the initial sensitization (prevention group) or at the end of sensitization period (treatment group). in whole-course intervention group, probiotics were administered 2 weeks before the initial sensitization until 1 week after the end of se ... | 2010 | 20846358 |
| effects of lactobacillus rhamnosus on zebrafish oocyte maturation: an ftir imaging and biochemical analysis. | the aim of this study was to verify the effects of probiotic lactobacillus rhamnosus on zebrafish oocyte maturation using fpa (focal plane array) ftir imaging together with specific biochemical assays (sds-page, real-time pcr and enzymatic assay). oocyte growth is prevalently due to a vitellogenic process which consists of the hepatic synthesis of vitellogenin and its selective uptake during maturation. the administration of l. rhamnosus imc 501 for 10 days induced chemical changes to oocyte com ... | 2010 | 20936269 |
| impact of maternal diet during pregnancy and breastfeeding on infant metabolic programming: a prospective randomized controlled study. | to evaluate the impact of maternal diet and intensive dietary counselling during pregnancy and breastfeeding on the infant's metabolic status. | 2010 | 20948557 |
| probiotic properties of lactobacillus strains isolated from the feces of breast-fed infants and taiwanese pickled cabbage. | this study assessed potential probiotic lactobacillus strains isolated from the feces of breast-fed infants and from taiwanese pickled cabbage for their possible use in probiotic fermented foods by evaluating their (i) in vitro adhesive ability, resistance to biotic stress, resistance to pathogenic bacteria, and production of β-galactosidase; (ii) milk technological properties; and (iii) in vivo adhesive ability, intestinal survival and microbial changes during and after treatment. five lactobac ... | 2010 | 20951815 |
| functional analysis of the p40 and p75 proteins from lactobacillus casei bl23. | the genomes of lactobacillus casei/paracasei and lactobacillus rhamnosus strains carry two genes encoding homologues of p40 and p75 from l. rhamnosus gg, two secreted proteins which display anti-apoptotic and cell protective effects on human intestinal epithelial cells. p40 and p75 carry cysteine, histidine-dependent aminohydrolase/peptidase (chap) and nlpc/p60 domains, respectively, which are characteristic of proteins with cell-wall hydrolase activity. in l. casei bl23 both proteins were secre ... | 2010 | 21178363 |
| functional properties of lactobacillus strains isolated from kimchi. | the objective of this study was to evaluate the functional properties of lactic acid bacteria (lab) from kimchi, a traditional korean fermented vegetable product generally consumed raw as a side-dish with practically every meal. twelve mild acid producing facultatively heterofermentative lactobacillus strains were selected for their potential as starter cultures for fermentation of kimchi, and evaluated for their functional properties. eleven strains were identified as lactobacillus sakei and on ... | 2010 | 21215484 |
| survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method. | encapsulation of probiotic bacteria in cross-linked alginate beads is of major interest for improving the survivability in harsh acid and bile environment and also in food matrices. alginate micro beads (10-40 μm) containing the probiotics lactobacillus rhamnosus gg and lactobacillus acidophilus ncfm were produced by a novel technique based on dual aerosols of alginate solution and cacl(2) cross linking solution. extruded macro beads (approximately 2mm diameter) produced by the conventional meth ... | 2010 | 21276627 |
| lactic acid production from corn stover using mixed cultures of lactobacillus rhamnosus and lactobacillus brevis. | mixed cultures of lactobacillus rhamnosus and lactobacillus brevis was studied for improving utilization of both cellulose- and hemicellulose-derived sugars from corn stover for lactic acid production. during simultaneous saccharification and fermentation (ssf) of naoh-treated corn stover by the mixed cultures, a lactic acid yield of 0.70 g/g was obtained, which was about 18.6% and 29.6% higher than that by single cultures of l. rhamnosus and l. brevis, respectively. our results indicated that l ... | 2010 | 20943382 |
| [genetic identification of antagonistically active lactobacillus strains isolated from the oral cavity of healthy individuals]. | examination of dental deposits from 45 healthy individuals detected 3 lactobacillus strains showing a high antagonism toward test cultures. the api 50 ch "bio merieux" test systems were employed to identify strains as lactobacillus fermentum 39, lactobacillus rhamnosus 24 and lactobacillus paracasei 50. the results of analyzing the sequences of the 16s rrna genes of the test strains confirmed this identification, except for the latter strain. the taxonomic status of the third strain l. rhamnosus ... | 2010 | 21313755 |
| microbiological and physicochemical quality of fresh-cut apple enriched with the probiotic strain lactobacillus rhamnosus gg. | the effectiveness as protective culture of the probiotic lactobacillus rhamonosus gg (l. rham. gg) against salmonella and listeria monocytogenes on minimally-processed apples throughout storage as well as its effect on apple quality and natural microflora was evaluated. survival to subsequent exposure to gastric stress was also reported. apples were cut into wedges and dipped in a solution containing salmonella and l. monocytogenes (10(5) cfu ml(-1)) and/or l. rham. gg (10(8) cfu ml(-1)). apple ... | 2010 | 21056776 |
| lactobacillus gg as treatment for diarrhea during enteral feeding in critical illness: randomized controlled trial. | diarrhea is a common problem in critical illness. the aim of this study was to investigate the effect of probiotic treatment with lactobacillus rhamnosus gg on established diarrhea in critically ill patients. | 2010 | 20978244 |
| commensal-epithelial signaling mediated via formyl peptide receptors. | commensal bacteria and/or their products engender beneficial effects to the mammalian gut, including stimulating physiological cellular turnover and enhancing wound healing, without activating overt inflammation. in the present study, we observed commensal bacteria-mediated activation of the noninflammatory extracellular signal-regulated kinase[erk]/mitogen-activated protein kinase and akt signaling pathways in gut epithelial cells and delineated a mechanism for this bacterially activated signal ... | 2010 | 21037077 |
| manufacturing process influences properties of probiotic bacteria. | production and manufacturing methods and the food carrier may influence the properties of probiotic strains, and have an impact on the outcome of clinical intervention studies. the aim of the present study was to establish whether the properties of a specific probiotic strain, lactobacillus rhamnosus gg, may differ depending on the product and source of the strain. in total, fifteen different l. rhamnosus isolates, among them fourteen labelled as l. rhamnosus gg, were isolated from specific prob ... | 2010 | 21059281 |
| persistence of probiotic strains in the gastrointestinal tract when administered as capsules, yoghurt, or cheese. | most clinical studies of probiotics use freeze-dried, powdered bacteria or bacteria packed in capsules. however, probiotics are commercially available in various food matrices, which may affect their persistence in the gastrointestinal tract. the objective of the study was to compare oral and faecal recovery during and after administration of a combination of lactobacillus rhamnosus gg and lc705, propionibacterium freudenreichii subsp. shermanii js, and bifidobacterium animalis subsp. lactis bb1 ... | 2010 | 21074284 |
| a randomized controlled trial of lactobacillus gg in children with functional abdominal pain. | our aim was to determine whether lactobacillus rhamnosus gg (lgg) relieves symptoms in children with recurrent abdominal pain. | 2010 | 21078735 |
| malt in combination with lactobacillus rhamnosus increases concentrations of butyric acid in the distal colon and serum in rats compared with other barley products but decreases viable counts of cecal bifidobacteria. | several substances, including glutamine and propionic acid but in particular butyric acid, have been proposed to be important for colonic health. β-glucans lead to the formation of comparatively high amounts of butyric acid, and germinated barley foodstuff obtained from brewer's spent grain (bsg), containing high amounts of β-glucans and glutamine, has been reported to reduce the inflammatory response in the colon of patients with ulcerative colitis. the present study examines how 3 barley produ ... | 2010 | 21106924 |
| lactobacillus gg treatment during pregnancy for the prevention of eczema: a randomized controlled trial. | probiotic supplementation in early life may be effective for preventing eczema. previous studies have suggested that prenatal administration may be particularly important for beneficial effects. | 2010 | 21121927 |
| interaction of orally administered lactobacillus rhamnosus gg with skin and gut microbiota and humoral immunity in infants with atopic dermatitis. | the intestinal mucosa functions as a defence barrier against gut intraluminar antigens. the maturational events in the gut parallel its step-wise colonization. atopic dermatitis (ad) is associated with aberrant barrier functions of the skin epithelium and, in a subgroup of patients, of the gut mucosa. | 2010 | 21121981 |
| widely distributed lysogeny in probiotic lactobacilli represents a potentially high risk for the fermentative dairy industry. | prophages account for most of the genetic diversity among strains of a given bacterial species, and represent a latent source for the generation of virulent phages. in this work, a set of 30 commercial, collection and dairy-isolated lactobacillus casei group strains were used. a species-specific pcr assay allowed a reclassification, mainly of strains previously considered lactobacillus casei, into either lactobacillus paracasei or lactobacillus rhamnosus. all the strains were induced with mitomy ... | 2010 | 21131090 |
| use of lactulose as prebiotic and its influence on the growth, acidification profile and viable counts of different probiotics in fermented skim milk. | lactulose can be considered as a prebiotic, which is able to stimulate healthy intestinal microflora. in the present work, the use of this ingredient in fermented milk improved quality of skim milk fermented by lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus bulgaricus and bifidobacterium lactis in co-culture with streptococcus thermophilus. compared to control fermentations without lactulose, the addition of such a prebiotic in skim milk increased the counts of all probiotics, ... | 2010 | 21144608 |
| proteomics and transcriptomics characterization of bile stress response in probiotic lactobacillus rhamnosus gg. | lactobacillus rhamnosus gg (gg) is a widely used and intensively studied probiotic bacterium. although the health benefits of strain gg are well documented, the systematic exploration of mechanisms by which this strain exerts probiotic effects in the host has only recently been initiated. the ability to survive the harsh conditions of the gastrointestinal tract, including gastric juice containing bile salts, is one of the vital characteristics that enables a probiotic bacterium to transiently co ... | 2010 | 21078892 |
| probiotic manipulation of the gastrointestinal microbiota. | in a recent publication we examined whether high abundance of a probiotic species, lactobacillus casei subsp. rhamnosus gg (lgg), impacted the overall composition of the gastrointestinal (gi) microbiota of six-month-old infants at high risk for asthma development. profound gi microbiota restructuring and the establishment of significantly more even and putatively, functionally redundant consortia were characteristic of high lgg abundance. here we discuss, in the context of more recently publishe ... | 2010 | 21327043 |
| manufacture and characterization of functional emmer beverages fermented by selected lactic acid bacteria. | autochthonous lactic acid bacteria from emmer flour were screened based on the kinetic of acidification and used to ferment beverages containing emmer flour, emmer gelatinized flour, and emmer malt at percentages ranging 5-30% (wt/wt). preliminarily, the concentration of raw flour and malt was selected based on sensory analysis. different protocols were set up for the manufacture of four different beverages which used lactobacillus plantarum 6e as the starter. emmer beverages were mainly differe ... | 2010 | 21356461 |
| microencapsulated lactobacillus rhamnosus gg powders: relationship of powder physical properties to probiotic survival during storage. | freeze-dried commercial lactobacillus rhamnosus gg (lgg) were encapsulated in an emulsion-based formulation stabilized by whey protein and resistant starch and either spray-dried or freeze-dried to produce probiotic microcapsules. there was no difference in loss of probiotics viability after spray drying or freeze drying. particle size, morphology, moisture sorption, and water mobility of the powder microcapsules were examined. particle size analysis and scanning electron microscopy showed that ... | 2010 | 21535593 |
| exopolysaccharides of lactobacillus rhamnosus gg form a protective shield against innate immune factors in the intestine. | probiotic bacteria are administered as live microorganisms to provide a health benefit to the host. insight into the adaptation factors that promote the survival and persistence of probiotics in the gastrointestinal tract (git) is important to understand their performance. in this study, the role of the long galactose-rich exopolysaccharides (eps) of the prototypical probiotic strain lactobacillus rhamnosus gg (lgg) was investigated. in a competition experiment with wild type, the isogenic eps m ... | 2010 | 21375696 |
| folate content and retention in selected raw and processed foods. | adequate intake of folate reduced the risk of abnormalities in early embryonic brain development such as the risk of malformations of the embryonic brain/spinal cord, collectively referred to as neural tube defects (ntds). folate is extremely sensitive to destruction by heat, oxidation and uv light. the purpose of this study was to evaluate the use of different extraction procedures and enzymatic treatment to determine folate concentrations in variety of foods using a microbiological assay (ma) ... | 2010 | 21612148 |
| lactobacillus probiotic use in cardiothoracic transplant recipients: a link to invasive lactobacillus infection? | organisms contained in probiotics are generally regarded as non-pathogenic and safe to administer. however, increasing reports of probiotic-associated infection raise concern over the safety of these products. we report a case of lactobacillus empyema in a human immunodeficiency virus-infected lung transplant recipient receiving a probiotic containing lactobacillus rhamnosus gg. we compare the epidemiology of lactobacillus infections in heart and lung transplant recipients at our institution bef ... | 2010 | 21040283 |
| antimicrobial susceptibility of lactobacillus rhamnosus. | we aimed to determine the minimum inhibitory concentrations (mics) of lactobacillus rhamnosus (n=75) strains, to study their antibiotic resistance genes with microarray, and to assess the microbiological cut-off values of tested antimicrobial agents. l. rhamnosus strains were tested with agar dilution, broth microdilution and etest methods for ampicillin, clindamycin, erythromycin, gentamicin, streptomycin, and tetracycline using specific lsm medium. most of the l. rhamnosus strains were found p ... | 2010 | 21831752 |
| adaptation factors of the probiotic lactobacillus rhamnosus gg. | probiotic bacteria are administered as live micro-organisms to provide a health benefit to the host. knowledge on adaptation factors that promote the survival and persistence of probiotics in the intestine is key to understand and improve their ecological and probiotic performance. adaptation factors include adhesins, molecules conferring stress tolerance and nutritional versatility, antimicrobial products against competing microbes, and factors promoting resistance against the host immune syste ... | 2010 | 21831772 |
| expression of clpl1 and clpl2 genes in lactobacillus rhamnosus vtt e-97800 after exposure to acid and heat stress treatments or to freeze-drying. | the aim of the study was to evaluate the potential of utilising the information on expression levels of selected stress genes in assessing the quality of probiotic products. for this purpose rt-qpcr methods were developed to study the expression of clpl1 and clpl2 stress genes in lactobacillus rhamnosus vtt e-97800 (e800) cells after exposure to processing-related stress conditions or to freeze-drying. heat treatments in laboratory scale were performed with e800 cells incubated at 47 -¦c or 50 - ... | 2010 | 21831761 |
| effect of dietary probiotics on clownfish: a molecular approach to define how lactic acid bacteria modulate development in a marine fish. | we set out to determine whether probiotic addition would improve larval development in the false percula clownfish amphiprion ocellaris and to determine what molecular responses could be observed in the larvae following probiotic exposure. we supplied the probiotic bacterial strain lactobacillus rhamnosus imc 501 to clownfish larvae from the first day posthatch simultaneously by live prey and with addition to rearing water (group 2) and exclusively by live prey (group 3). we observed twofold hig ... | 2010 | 19923354 |
| lactobacillus rhamnosus hepatic abscess associated with mirizzi syndrome: a case report and review of the literature. | the clinical significance of lactobacillus spp. isolated from clinical specimens has often been overlooked due to its low virulence. we report the first case of life-threatening bacteremic liver abscess due to lactobacillus rhamnosus associated with mirizzi syndrome in a 74-year-old chinese man. literature on sporadic reports of lactobacillus liver abscess is reviewed. | 2010 | 19766429 |
| mucosal adhesion properties of the probiotic lactobacillus rhamnosus gg spacba and spafed pilin subunits. | lactobacillus rhamnosus gg is a well-established gram-positive probiotic strain, whose health-benefiting properties are dependent in part on prolonged residence in the gastrointestinal tract and are likely dictated by adherence to the intestinal mucosa. previously, we identified two pilus gene clusters (spacba and spafed) in the genome of this probiotic bacterium, each of which contained the predicted genes for three pilin subunits and a single sortase. we also confirmed the presence of spacba p ... | 2010 | 20118368 |
| probiotics prevent death caused by citrobacter rodentium infection in neonatal mice. | intestinal bacterial infections are a major cause of morbidity and mortality, especially in neonates. therefore, the aims of this study were to characterize citrobacter rodentium infection in neonatal mice and determine the role played by specific probiotics in ameliorating disease severity. | 2010 | 19961304 |
| lactobacillus gg in the prevention of gastrointestinal and respiratory tract infections in children who attend day care centers: a randomized, double-blind, placebo-controlled trial. | the aim of our study was to investigate the role of lactobacillus gg (lgg) in the prevention of gastrointestinal and respiratory tract infections in children who attend day care centers. | 2010 | 19896252 |
| lung abscess and pleuritis caused by lactobacillus rhamnosus in an immunocompetent patient. | a 79-year-old man consulted us because of left chest pain and fever ranging from 38.0 to 38.9 °c. a chest computed tomography scan showed a mass lesion (φ40 mm) in the left lingular segment, and inflammatory markers were elevated. he was admitted with a diagnosis of lung abscess, and panipenem/betamipron was administered at a dose of 2 g/day, after which the symptoms showed slight transient resolution. however, his body temperature increased again, to more than 39.0 °c, on the eighth day of hosp ... | 2010 | 20072798 |
| oral treatment with probiotics reduces allergic symptoms in ovalbumin-sensitized mice: a bacterial strain comparative study. | evidence demonstrating an important role of the intestinal microbiota in the incidence of allergic disorders has led to the concept of using probiotics as possible antiallergic therapy. this study aimed to select a bacterial strain with the best antiallergic treatment effects from a panel of 6 bacterial strains in a mouse model of ovalbumin(ova)-allergic asthma. | 2010 | 19752564 |
| effect of natural commensal-origin dna on toll-like receptor 9 (tlr9) signaling cascade, chemokine il-8 expression, and barrier integritiy of polarized intestinal epithelial cells. | the intestinal epithelium is constantly exposed to high levels of genetic material like bacterial dna. under normal physiological conditions, the intestinal epithelial monolayer as a formidable dynamic barrier with a high-polarity structure facilitates only a controlled and selective flux on components between the lumen and the underlining mucosa and even is able to facilitate structure-based macromolecules movement. the aim of this study was to test the effect of natural commensal-origin dna on ... | 2010 | 19714766 |
| intermediate chains of exopolysaccharides from lactobacillus rhamnosus rw-9595m increase il-10 production by macrophages. | to evaluate the immunosuppressive properties of the exopolysaccharide (eps) from high-eps producer lactobacillus rhamnosus rw-9595m on inflammatory cytokines produced by macrophages. | 2010 | 19702865 |
| efficacy of whey protein gel networks as potential viability-enhancing scaffolds for cell immobilization of lactobacillus rhamnosus gg. | this study investigated cell immobilization of lactobacillus rhamnosus gg in three separate protein products: native, denatured and hydrolysed whey protein isolate (wpi). treatments were assessed for their ability to enhance probiotic survival during storage, heat stress and ex vivo gastric incubation. spatial distribution of probiotic cells within immobilized treatments was evaluated by atomic force and confocal scanning laser microscopy, while cell viability was enumerated by plate count and f ... | 2010 | 20045713 |
| lactobacillus rhamnosus gg induces tumor regression in mice bearing orthotopic bladder tumors. | the present gold standard for bladder cancer is mycobacterium bovis, bacillus calmette guerin (bcg) immunotherapy. but it has a non-responder rate of 30-50% and side effects are common. lactobacillus casei strain shirota has been reported to reduce the incidence of recurrence in bladder cancer patients and to cure tumor-bearing mice. our aim was to determine if lactobacillus rhamnosus gg (lgg) could be as efficacious as bcg in a murine model of bladder cancer. mb49 bladder cancer cells secreting ... | 2010 | 20015287 |
| preferential promotion of apoptosis of monocytes by lactobacillus casei rhamnosus soluble factors. | inflammatory bowel disease (ibd) is characterized by dense infiltrates of and defective apoptosis by mucosal cell populations. some probiotics inhibit monocytes' expansion, although mechanisms remain unknown. supernatants of lactobacillus strains were investigated for inducing apoptosis of monocytes. | 2010 | 19665262 |
| probiotics and prebiotics in atopic dermatitis: review of the theoretical background and clinical evidence. | the prevalence of atopic dermatitis (ad) has risen over the past decades, especially in western societies. according to the revised hygiene hypothesis this increase is caused by a changed intestinal colonization pattern during infancy, which has an impact on the immune system. manipulating the intestinal microflora with pro-, pre- or synbiotics is an innovative way to prevent or treat ad. this review provides an overview of the theoretical basis for using probiotics and prebiotics in ad and pres ... | 2010 | 19573143 |
| lactobacilli inhibit shigella dysenteriae 1 induced pro-inflammatory response and cytotoxicity in host cells via impediment of shigella-host interactions. | shigella dysenteriae type 1 dysentery is a major cause of morbidity and mortality in children from less developed and developing countries. the present study explores the hypothesis that lactobacilli protect the host cell during s. dysenteriae type 1 infection and its mechanism of action. | 2010 | 19535308 |
| cow's milk allergy is associated with changes in urinary organic acid concentrations. | cow's milk allergy (cma) is the most common form of food allergy affecting 2.5% of children, but the diagnosis is often difficult. both intestinal microbiota and barrier function seem to be disturbed in patients with food allergies, and administration of probiotics has been shown to normalize intestinal microbiota and alleviate symptoms. we hypothesized that the differences in intestinal metabolic activity and permeability could lead to detectable changes in the end-products of metabolism in pat ... | 2010 | 19397753 |
| evaluation of lactobacillus rhamnosus gg using an escherichia coli k88 model of piglet diarrhoea: effects on diarrhoea incidence, faecal microflora and immune responses. | probiotic bacterium lactobacillus rhamnosus gg (lgg) has been demonstrated to adhere to pig intestinal mucus, and is able to displace and inhibit pathogens, including escherichia coli (e. coli), in vitro. however, currently there are few data concerning the effects of lgg on piglet health. the objectives of this study were to assess the effectiveness of lgg in reducing the incidence and severity of post-weaning diarrhoea in piglets, and to investigate its mechanisms of action. eighteen weaned ba ... | 2010 | 19782483 |
| probiotic yogurt consumption is associated with an increase of cd4 count among people living with hiv/aids. | to evaluate the long term effect of yogurt supplemented with lactobacillus rhamnosus fiti on the immune function (cd4 count) of people living with hiv/aids. | 2010 | 20463586 |
| oral lactoferrin for the prevention of sepsis and necrotizing enterocolitis in preterm infants. | lactoferrin, a normal component of human colostrum, milk, tears and saliva can enhance host defence and may be effective in the prevention of sepsis and necrotizing enterocolitis (nec) in preterm neonates. | 2010 | 20464748 |
| probiotic prophylaxis of ventilator-associated pneumonia: a blinded, randomized, controlled trial. | enteral administration of probiotics may modify the gastrointestinal environment in a manner that preferentially favors the growth of minimally virulent species. it is unknown whether probiotic modification of the upper aerodigestive flora can reduce nosocomial infections. | 2010 | 20522788 |
| capsular and slime-polysaccharide production by lactobacillus rhamnosus jaas8 isolated from chinese sauerkraut: potential application in fermented milk products. | exopolysaccharides (epss) produced by lactic acid bacteria (lab) play an important role in the improvement of the physical properties of fermented dairy products. to find eps-producing lab strains with potential industrial applications, a lactobacillus rhamnosus strain, l. rhamnosus jaas8, that is capable of producing two forms of eps when grown in mrs broth or semi-defined medium with glucose as a carbon source was isolated and identified from chinese sauerkraut. the capsular-polysaccharide (cp ... | 2010 | 20541116 |
| probiotics in pregnant women to prevent allergic disease: a randomized, double-blind trial. | previous reports have suggested that certain probiotics given to mothers and children at risk of atopy halves the incidence of atopic dermatitis (ad) at 2 years of age. | 2010 | 20545688 |
| fecal bacteriotherapy for relapsing clostridium difficile infection in a child: a proposed treatment protocol. | clostridium difficile infection (cdi) is a potentially serious emerging infectious disease. the incidences of cdi in childhood and cdi cases complicated by relapses have increased by 50% or more in north america during the past 2 decades. we report here the case of a 2-year-old child with relapsing cdi caused by the epidemic strain bi/nap1/o27 that was refractory to saccharomyces boulardii and lactobacillus rhamnosus gg probiotics and to intensive therapy with traditional (metronidazole, vancomy ... | 2010 | 20547640 |
| lactobacillus rhamnosus gr-1 and l. reuteri rc-14 to prevent or cure bacterial vaginosis among women with hiv. | to assess, among women with hiv, whether long-term oral lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 supplementation can prevent bacterial vaginosis (bv) and enhance the cure rate of metronidazole among those with bv. | 2010 | 20801446 |
| cloning, expression, purification, and activity assay of proteins related to d-lactic acid formation in lactobacillus rhamnosus. | two proteins that might be responsible for d-lactic acid (d-la) formation were screened from the genome database of lactobacillus rhamnosus gg. the coding genes of the two proteins in l. rhamnosus casl, ldhd1 and ldhd2, were cloned and expressed in escherichia coli rosetta with an inducible expression vector petduet-1 (novagen, darmstadt, germany), respectively. the two purified proteins, ldhd-1 and ldhd-2, migrated as a single protein band separately, both corresponding to an apparent molecular ... | 2010 | 20549201 |
| synthesis of isoflavone aglycones and equol in soy milks fermented by food-related lactic acid bacteria and their effect on human intestinal caco-2 cells. | one hundred and three strains of lactic acid bacteria, isolated from various food ecosystems, were assayed for β-glucosidase activity toward p-nitrophenyl-β-d-glucopyranoside substrate. lactobacillus plantarum dppma24w and dppmasl33, lactobacillus fermentum dppma114, and lactobacillus rhamnosus dppmaaz1 showed the highest activities and were selected as the mixed starter to ferment various soy milk preparations, which mainly differed for chemical composition, protein dispersibility index, and si ... | 2010 | 20822177 |
| immune-stimulating effects of lactic acid bacteria in vivo and in vitro. | the health-promoting effects of lactic acid bacteria (lab) are well recognised, making them a popular functional food ingredient. commercially available probiotic products are often promoted as capable of improving immune defences also in healthy subjects. however, while strain-specific differences exist in the effects of lab, conventional yoghurt bacteria have proved beneficial as well. for comparing the immunological effects of conventional and probiotic lab, young healthy women received eithe ... | 2010 | 20550748 |
| improvement of l(+)-lactic acid production from cassava wastewater by lactobacillus rhamnosus b 103. | l(+)-lactic acid is used in the pharmaceutical, textile and food industries as well as in the synthesis of biodegradable plastics. the aim of this study was to investigate the effects of different medium components added in cassava wastewater for the production of l(+)-lactic acid by lactobacillus rhamnosus b 103. | 2010 | 20564419 |
| long-term vaginal administration of lactobacillus rhamnosus as a complementary approach to management of bacterial vaginosis. | to investigate the advantages of long-term vaginal administration of lactobacillus rhamnosus after oral treatment with metronidazole to prevent the recurrence of bacterial vaginosis (bv). | 2010 | 20573348 |
| randomized controlled trial of probiotics for the prevention of spontaneous preterm delivery associated with intrauterine infection: study protocol. | abstract: background: spontaneous preterm deliveries that occur before the 34th week of gestation, and particularly before the 32nd week of gestation, have been strongly associated to intrauterine infection, ascending from vagina, and represent the largest portion of neonatal deaths and neurological problems. bacterial vaginosis, characterized by a diminished or absent flora of lactobacilli and increased colonization of several anaerobic or facultative microorganisms, increases two times the ris ... | 2010 | 20591191 |