sensitivity of crude and purified fasciola antigens in immunological diagnosis of human fascioliasis.a study has been done to find the effect of purification of crude antigens, extracted from adult fasciola gigantica and fasciola hepatica worms on the sensitivity of some serological techniques in diagnosis of fascioliasis. using c.i.e.p. test, the partially purified antigens of both fasciola species were less sensitive than their corresponding crude ones. using i.h.a., the difference between results of crude and partially purified antigens for the two species were statistically insignificant, b ...19892768848
detection of fasciola gigantica eggs in faeces: a comparison of methods. 19873824857
experimental cholecystitis in goats caused by mature fasciola gigantica.mature fasciola gigantica obtained from naturally infected cattle were surgically transferred into the gallbladders of six fluke-free goats. two of the goats died at two and 19 weeks and the remaining four were slaughtered at 12, 48, 48 and 80 weeks after the transfer. nine of 20 transferred f gigantica were recovered from the gallbladder and common hepatic duct of the experimental goats. the walls of gallbladders and bile ducts were thickened by epithelial proliferation, fibrosis and mononuclea ...19854012032
a new technique for the recovery of fasciola gigantica eggs from cattle faeces. 19674887044
environmental stress and parasitic infections: i. the effects of gramoxone and hexadrin on embryo and hatching of fasciola gigantica miracidia.the effect of two chemical compounds commonly used in agriculture, gramoxone (a herbicide) and hexadrin (an insecticide) on embryonic development and hatching of fasciola gigantica miracidia were experimentally assessed. these two pesticides were introduced in varying quantities into petri dishes containing unembryonated eggs of the trematode for a period of 30 days. lc50 (lethal concentration for 50% hatching) values were determined for them. for gramoxone it was 2 ppm and for hexadrin it was 2 ...19883202331
[epidemiologic data on distomiasis due to fasciola gigantica cobbold on the island of madagascar. infestation of cattle between 1975 and 1981].stools examinations of zebus were made in madagascar between 1975 and 1981 for determining the importance of fascioliasis gigantica in the six provinces of the island during this time. high percentages of f. gigantica eggs were found in the stools of the different infected zebus-herds of five provinces. no fasciolid infection was noted in 1979 in the cattle of the province of diego-suarez. differences were noted between the infection rate obtained by fecal examination and the rate indicated in t ...19854075467
protection against fasciola gigantica infection in rats administered metacercarial antigens.immature worms were recovered from the liver parenchyma of rats which received extracts of metacercariae or adult worms of fasciola gigantica, after challenge infection. when resistance was assessed by counting the number of immature worms, exposure of the animals to extracts of these two stages of the flukes conferred a significant degree of protection against challenge with f gigantica metacercariae. rats given the extract of metacercariae were more resistant to infection than rats immunised w ...19854081346
the role of carbohydrates in the biology of the liver fluke, fasciola gigantica: on the active absorption of glucose. 19744465012
the effect of niclofolan on desert sheep experimentally infected with immature fasciola gigantica.eight desert sheep were each infected orally with 500 metacercariae of fasciola gigantica and, after 4 weeks, four of the animals were given niclofolan orally at the recommended therapeutic dose rate of 7 mg/kg, the other four remaining as controls. one week later, the animals were slaughtered and the fasciocidal effect of the drug was evaluated on the basis of worm burden, haemogram, some plasma constituents, and gross and histopathological lesions of the liver, as indicators of efficacy. the t ...19854094030
lymnaea natalensis laboratory culture and production of fasciola gigantica metacercariae. 19684905987
studies on the energy reserve of fasciola gigantica from nigerian zebu energy reserves in the body tissue of mature liver flukes var. fasciola gigantica from the bile ducts of nigerian zebu cattle the following percentages were found: protein 60.29, lipid 11.92, carbohydrate 7.0. the analysis of the lipid fractions showed the following composition: 47% phospholipids (63% phosphatidyl choline, 28% phosphatidyl serine, 9% lysophosphatidyl choline) and 22% triglycerides, 16% fatty acids, 6% cholesterol, 5% monoacyl glycerol, and 4% cholesterol ester.19854038247
preliminary observations on antigen activity of fasciola gigantica by immunodiffusion test in sheep. 19744212259
[distomatosis of the domestic rabbit caused by fasciola gigantica]. 19744469355
biochemical and histochemical studies on the non-specific phosphomonoesterases of fasciola gigantica cobbold 1855. 19724336211
note on the sporocyst in fasciola gigantica. 19744471455
notes on glycogen deposits in limnaea auricularia var. rufescens and the parasitic larval stages of fasciola gigantica and echinostoma revolutum. a histochemical study. 19684190113
studies on heterologous resistance between schistosoma bovis and fasciola gigantica in sudanese cattle.using the local strains of schistosoma bovis and fasciola gigantica, it was shown that sudanese zebu calves with mature primary infections of f. gigantica were highly resistant to challenge with s. bovis cercariae, and vice versa. liver enzyme tests showed that, in both cases, the primary infections had caused some liver damage. primary infection with irradiated s. bovis cercariae, which did not cause significant liver damage, did not protect significantly against challenge with f. gigantica.19863701021
some biochemical indices in naturally occurring fascioliasis in goats.some biochemical indices were assayed in goats naturally harbouring fasciola gigantica infection and compared with uninfected control goats. infected goats had significantly lower levels of serum glucose (47.6 +/- 1.8 mg dl-1) and albumin (3.1 +/- 0.1 g dl-1) and reduced albumin:globulin ratio (1.1 +/- 0.1). total lipid (526.8 +/- 2.4 mg dl-1), serum glutamate dehydrogenase (15.3 +/- 0.9 iu litre-1) and serum alkaline phosphatase (31.6 +/- 1.9 kind's and king's unit dl-1) were high in infected g ...19863704349
studies on naturally-occurring ovine fascioliasis in the sudan.haematological, biochemical and pathological changes were investigated in 214 sheep naturally infected with fasciola gigantica in an endemic area in the sudan together with 82 uninfected controls. infected animals showed a clear decrease in erythrocyte counts, haemoglobin concentration and packed cell volume, a normochromic, normocytic anaemia, leucocytosis and eosinophilia. serum concentrations of the enzymes glutamate dehydrogenase, sorbitol dehydrogenase and glutamate oxaloacetic acid transam ...19863701020
trials with rafoxanide. 5. efficacy studies against fasciola hepatica, fasciola gigantica, paramphistomum microbothrium and various nematodes in sheep. 19724670768
[fasciola gigantica infestation in small laboratory animals]. 19744838609
[case of hepatic distomatosis due to fasciola gigantica. diagnosis in the early stage of bilharziosis due to schistosoma mansoni]. 19684922836
hepatic calcification due to fasciola gigantica. 19744808568
[apropos of a case of hepatic distomatosis due to fasciola gigantica contracted in cameroon]. 19684973929
[study on hypersensitivity, precipitating antibodies and complement fixing antibodies during bovine infestation by fasciola gigantica]. 19715002886
a comparison of the activity of some fasciolicides against immature fasciola gigantica in experimentally infected calves. 19715153728
the life cycle of fasciola gigantica cobbold, 1885 i. the sporocyst myth? 19715153727
[role of nutritional factor in bovine and ovine distomatosis caused by fasciola gigantica]. 19715153729
[method of evaluation of the effects of various anthelmintic drugs in relation to the immature forms of fasciola gigantica]. 19715153730
the experimental demonstration of lymnaea lagotis euphratica as an intermediate host of fasciola gigantica in iraq. 19705500109
[apropos of the action of bitin-s and of bromophenophos on immature fasciola gigantica]. 19715106008
vertical distribution of fasciola gigantica metacercariae on stems of rice plant grown in a water pot. 19744444734
human infection with the liver fluke fasciola gigantica. 19744422226
trials with rafoxanide. 2. efficacy against fasciola gigantica in cattle. 19715161408
trials with rafoxanide. 1. fasciola gigantica in cattle in angola. 19715161407
oxyclozanide--activity against fasciola gigantica in naturally infected buffalo, cattle, sheep and goats. 19715164589
a preliminary note on the susceptibility, prepatency and recovery of fasciola gigantica in small laboratory animals. 19724641407
[anthelmintic activity of a halogen derivative of salicylanilide against fasciola gigantica]. 19715169494
liver fluke fasciola gigantica in african buffalo and antelopes in uganda, east africa. 19724673717
the pathogenic effect of experimental infections with fasciola gigantica in cattle. 19744434159
[therapy and early diagnosis of fasciola gigantica infection in sheep]. 19695388487
experimental infection of calves with fasciola gigantica. 19695362596
a type of cercaria easily mistaken for fasciola gigantica in uganda. 19695386286
the occurrence of fasciola gigantica in the liver of an indian rhinoceros (r. unicornis). 19715103964
[the effect of hetol on immature and mature stages of fasciola gigantica in artificially infected sheep]. 19675628510
[distomatosis in madagascar. the fasciola gigantica cycle]. 19675625568
[effectiveness of zanil against immature and sexually mature stages of fasciola gigantica in artifically infected sheep]. 19685692951
[a case of human fasciola gigantica douve. simultaneous hepatic and subcutaneous infestation]. 19734801860
[phacochoerus aethiopicus (pallas), new host of fasciola gigantica cobbold, 1855]. 19734801778
[distomiasis in madagascar: use of molluscacides in the campaign against lymnaea natalensis hovarum (intermediate host of fasciola gigantica)]. 19695407991
[the effect of bilevon m against immature and mature stages of fasciola gigantica in artificially infected sheep]. 19685693411
i. protein and polysaccharide content of fasciola gigantica skin test antigens and their reactivity in the diagnosis of bilharziasis. 19685702063
[some data on comparative biochemical and immunological studies of the number of antigenic preparations from fasciola hepatica and fasciola gigantica]. 19685712600
[hepatic distomatosis due to fasciola gigantica (tropical fluke). importance of psychic problems]. 19685688554
ii. fractionation of the antigen of fasciola gigantica by salting out with reference to the use of the isolated fractions as skin test antigens in cases of urinary schistosomiasis. 19685726942
3. fractionation of the antigen of fasciola gigantica by electrophoresis and the use of the fractions in the intradermal test for diagnosing bilharziasis. 19685726943
studies on the activity of nitroxynil against fasciola gigantica in naturally infected buffaloes, cattle and sheep. 19695387806
suitability of extracts of fasciola gigantica for diagnosis of schistosomal cases. 19685736436
a note on dicrocoeliasis and fasciola gigantica infection in livestock in northern ghana, with a record of spurious and of genuine dicrocoelium hospes infections in man. 19666006926
comparison of hemoglobins of fasciola gigantica (trematoda: digenea) and its host. 19676026225
the enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths.the activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in crithidia fasciculata, trypanosoma cruzi, leishmania major, trichomonas vaginalis, eimeria tenella, toxoplasma gondii, plasmodium berghei, fasciola gigantica, schistosoma mansoni, hymenolepis diminuta, nippostrongylus brasiliensis and trichuris muris. the m ...19816111750
[fasciola gigantica (tropical fluke) hepatic distomatosis. importance of the mental disorders]. 19675599911
[differences between the testicular morphology of fasciola hepatica and fasciola gigantica]. 19705535130
[treatment in tropical africa of hhepatobiliary fascioliasis caused by fasciola gigantica in zebu cattle. value of bilevon r bayer]. 19705534364
dynamics of lymnaea natalensis populations in the zaria area (nigeria) and the relation to fasciola gigantica infections.observations on the seasonal changes in the lymnaea natalensis population and the infection rate of these snails with fasciola gigantica during 1972-1976 are reported. the data were obtained from snails in three different locations near zaria in northern nigeria. one of the survey sites dried out during the drought of 1972-1974 and had to be abandoned. the snail population showed seasonal variations with the lowest number of snails at the very end of the dry season. many juvenile snails develope ...19806106353
the pathology of experimental infection of cattle with fasciola gigantica. 19705526751
[additional data on superficial argentophilic structures of the miracidium and cercaria of fasciola gigantica (cobbold, 1855) (trematoda:fasciolidae) and on the epidemiology of this parasite].the life cycle of a senegalese strain of fasciola gigantica was carried out in france from adults recovered in the liver of naturally infected cattle (bos taurus). laboratory-reared lymnaea natalensis experimentally infected shed numerous cercariae. metacercariae were used for successful infection of sheep. in the miracidium, variations are observed in the disposition of the medio-ventral epithelial cell of the second tier. the distribution of the superficial argentophilic structures is similar ...19806164333
[case of infection with fasciola gigantica cobbold, 1856]. 19676063274
biology, life-tables, and intrinsic rates of increase of bulinus globosus morelet and lymnea natalensis kraus (gastropoda: pulmonata)--the snail intermediate hosts of schistosoma haematobium and fasciola gigantica, respectively, in southeastern nigeria.two tables and a figure are used to present the epidemiological and epizoological particulars of bulinus globosus and lymnaea natalensis, the intermediate hosts of schistosoma haematobium and fasciola gigantica in southeastern nigeria. possible ways of reducing the populations of these intermediate hosts are seen in the combined application of chemical agents and environmental shaping.19846529436
effect of the seasonal variations of temperature on the development of fasciola gigantica in the snail host in the kenya highlands. 19635889258
[3 cases of hepatic fasciolasis due to fasciola gigantica contracted in central africa]. 19685759965
chemosterilization and its reversal in the snail lymnaea acuminata.treatment of the snail lymnaea acuminata, the vector for the liver flukes fasciola hepatica and fasciola gigantica, with two alkylating agents (cyclophosphamide and busulfan) and one thiocarbamide (thiourea) caused reduction in the number of eggs and production of non-viable embryos. all the three drugs caused reduction in the levels of dna, rna and protein and the activity of alkaline phosphatase in the gonadal tissues of this snail. there was enhancement in free amino acid levels and activity ...19836189361
changes in blood cellular components, serum protein concentrations and serum enzyme activities in buffaloes infested with fasciola gigantica.of 510 adult buffaloes examined, 88 (17.3 per cent) were found to be suffering from fasciola gigantica infestation. there was a reduction in the haemoglobin, packed cell volume and red blood cell count in the fasciola affected buffaloes and an increase in their white blood cell count. there was no significant change in mean corpuscular volume, mean corpuscular haemoglobin or mean corpuscular haemoglobin concentration in the fasciola affected buffaloes. there was also a decrease in total serum pr ...19827146634
effect of bithionol on b-glucuronidase in serum and liver of rabbits infected with fasciola gigantica. 19836631102
histopathological changes in the liver of a spotted deer (axis axis) infected with fasciola gigantica. 19695813688
the occurrence of fasciola gigantica in the lungs of indian buffaloes (bos bubalis). 19655890486
osmotic and ionic regulation in fasciola gigantica (trematoda: digenea). 19665960789
[preliminary note on the presence of fasciola gigantica in madagascar]. 19665951730
on the comparative susceptibility of lymnaea natalensis (kraus) and l. rufescens (gray) to infection with fasciola gigantica (west african strain) and the tissue responses in the snails. 19665961523
effect of sodium benzene sulphonate on adults and eggs of lymnaea natalensis and miracidia of fasciola gigantica. 19827199774
study of phosphagens in two trematodes.extracts from the adults of two trematodes fasciola gigantica and paramphistomum explanatum have been shown to contain three phosphagens, phosphoarginine, phosphocreatine, and phosphoglycocyamine. the three phosphokinases involved in the transphosphorylation of these phosphagens in both worms were inhibited by edta and mercuric ions while iodoacetate had no effect.19806245542
use of a partially purified fasciola gigantica worm antigen in the serological diagnosis of human fascioliasis in egypt.crude extracts of fasciola gigantica adult worms, when used as an antigen in indirect hemagglutination (iha) and counterimmunoelectrophoresis (ciep) tests, detected all independently diagnosed human f. gigantica and f. hepatica infections but cross-reacted with sera of patients with schistosomiasis and amebiasis. fractionation of this crude worm extract using sephadex g-200 chromatography demonstrated four major protein peaks. antigen from the crest and descending portion of peak ii (mol. wt. ap ...19836305221
alpha esterase enzyme in liver of rabbits infected with fasciola gigantica and treated with bithionol. 19827153561
the effect of sex on seasonal variation in single and double infection of cattle in sierra leone by dicrocoelium hospes and fasciola gigantica.the incidence of dicrocoeliasis (61.8%) is higher than that of fascioliasis (25.5%) and their peaks do not occur at the same time of the year. the peak for the former occurs in the dry season while that of the latter occurs in the rainy season. the incidence of dicrocoeliasis and fascioliasis in female cattle was found to be higher than that of male cattle. double infection was higher in female (14.7%) than in male hosts (9.6%).19846541838
[epidemiology of hepatic fascioliasis (fasciola gigantica) at the dairy farm of the lubumbashi (zaire) ranch. results of a deparasitization trial. recommendations]. 19836672883
infectivity and pathogenicity of fasciola gigantica in west african dwarf sheep and goats.the infectivity and pathogenicity of fasciola gigantica for sheep and goats were investigated in animals infected with single doses of between 200 and 2,000 metacercariae. acute fascioliasis occurred in sheep infected with 1,000 to 2,000 cysts in which 10 to 17% of the cysts became established. the infected animals died 80 to 90 days after infection. in goats given the same infective doses death from acute fascioliasis occurred earlier (70 to 83 days after infection) and 12 to 38% of cysts becam ...19846485107
activation of prophenol oxidase in the liver fluke fasciola gigantica cobbold.activation of prophenol oxidase of fasciola gigantica has been demonstrated using biochemical and electrophoretic techniques. based on the difference in the electrophoretic pattern of the proenzyme and activated enzyme, a probable mechanism of activation of the prophenol oxidase is suggested. a role for mehlis' gland in the activation process is suggested. the significance of the results is discussed in the light of related studies in insects.19826817288
bovine fascioliasis in nigeria--intercurrent parasitic and bacterial infections.the bacterial and parasitic infections associated with field cases of bovine fascioliasis due to fasciola gigantica were investigated; 47.6% of infected cattle had blood parasites, 61.9% had faecal-borne parasites and 85.8% had positive bacterial isolates in the bile in contrast to 13.3% with blood parasites, 71.1% with faecal-borne parasites and 28.9% positive for bile bacteria among uninfected cattle. trypanosoma spp., babesia, paramphistomum and schistosoma were the most commonly found parasi ...19827101464
specific inhibitors and substrates studies on the cholinesterases of fasciola gigantica from sheep and goats. 19836871931
studies on specific inhibitors and substrates of cholinesterases of fasciola gigantica from cattle and buffaloes. 19827180251
incidence of liver fluke infections (fasciola gigantica and dicrocoelium hospes) in ruminants in northern thousand and twenty-four cattle, 550 sheep and 1,748 goats slaughtered in a rural slaughter slab during 1973 to 1975 were examined for evidence of liverfluke infections. the prevalence rate of fasciola gigantica and dicrocoelium hospes infections was respectively 65.4% and 56.0% in cattle, 40.8 and 13.1% in sheep and 17.6 and 5.2% in goats. other trematodes detected were schistosoma bovis and paramphistomes. the seasonal incidence of f. gigantica as well as of d. hospes was highest during an ...19807414705
in vitro effect of nitroxynil (trodax) and rafoxanide on adult fasciola gigantica. 19817313227
[epidemiologic study of s. haematobium bilharziasis in the rice fields of yagoua (north cameroon) ii. distribution and ecology of intermediate hosts].while inventoring the local malacological fauna and evaluating the density of species acting as intermediate hosts of human schistosomes it has been shown that bulinus truncatus is predominant in the study area. bulinus globosus is scarcely encountered. biomphalaria pfeifferi is only found in the mayo guerleo, in irrigation canals and draining ditches. but b. globosus and b. pfeifferi have not yet invaded the recent aquatic network and are not found in the lake nor in the newly created irrigatio ...19827201892
igg response in natural and experimental infections of cattle with fasciola gigantica.a specific igg response was detectable in serum and bile of cattle infected with fasciola gigantica. although there was an increase in both specific and total igg concentration in the sera of fluke-infected cattle, there was no significant correlation between igg1 concentration and the worm burden (r = 0.17; p greater than 0.05) in chronic fascioliasis. however, there was a highly significant correlation between the levels of igg1 in the serum and bile of infected cattle (r = 0.87; p less than 0 ...19836686385
susceptibility of different species of lymnaea snails to miracidia of fasciola gigantica and f. hepatica in iran. 19807217650
the epidemiology of fascioliasis in malawi. part ii. epidemiology in the definitive host.the epidemiology of fasciola gigantica cobbold was studied in the definitive host and the epidemiological cycle was determined. the incidence of immature flukes was high in september to december and mature flukes from december to march. a survey of chronic fascioliasis was conducted covering almost the whole country. the prevalence of the disease was high in the northern and central regions. based on the epidemiological findings a control programme for the disease in malawi was prepared.19817256868
separation of outer cysts from metacercariae of fasciola gigantica in an aqueous two-phase system.the outer cysts (oc) of fasciola gigantica metacercariae (mc) were separated in an aqueous two-phase system composed of polyethylene glycol and dextran in distilled water. a large number of separated mc without oc were recovered in the bottom layer rich in dextran in the system. ten separated mc in the layer were used to inoculate the mouse with a syringe and juveniles were recovered from the animals on the 14th day. when the number of juveniles recovered was compared with that from the mice inf ...19957622796
red blood cell survival and faecal clearance in sheep infected with fasciola gigantica. 19817320242
efficacy of ivermectin against internal parasites of sheep.ivermectin in an oral formulation was evaluated by the non-parametric method against a wide range of endoparasites of sheep in 15 trials involving a total of 297 sheep. ivermectin at 200 micrograms/kg was more than 80% effective in more than 80% of the treated animals (i.e. class a) against induced infestations of 3rd and 4th stage larvae and adults of chabertia ovina, dictyocaulus filaria, haemonchus contortus (including a benzimidazole-resistant strain), nematodirus spathiger, oesophagostomum ...19846549472
[immunodiagnosis of fasciola gigantica fascioliasis by elisa technique in senegal. preliminary observations in 2 lambs].the purpose of this work was to investigate the use of the elisa test for the diagnosis of fasciolosis (fasciola gigantica) in sheep, in senegal, using fasciola gigantica metabolic excretion-secretion products as antigens. coprological analysis results were negative until the fourteenth week following infection; using the elisa test, the first anti-fasciola gigantica antibodies can be detected as early as the fourth week following infection.19947709030
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