Publications

study on the pupal morphogenesis of chrysomya rufifacies (macquart) (diptera: calliphoridae) for postmortem interval estimation.	chrysomya rufifacies (macquart) is one of the most common species of blow flies at the scene of death in southern china. pupae are useful in postmortem interval (pmi) estimation due to their sedentary nature and longer duration of association with the corpse. however, to determine the age of a pupa is more difficult than that of a larva, due to the fact that morphological changes are rarely visible during pupal development. in this study, eggs of c. rufifacies were reared in climatic chambers un ...	2015	26112814
laboratory colonization of the blow flies, chrysomya megacephala (diptera: calliphoridae) and chrysomya rufifacies (diptera: calliphoridae).	chrysomya megacephala (f.) and chrysomya rufifacies (macquart) were colonized so that larval growth rates could be compared. colonies were also established to provide insight into the protein needs of adult c. rufifacies and developmental rates of the ensuing larvae. the c. megacephala and c. rufifacies laboratory colonies were reared for five and six generations, respectively, at 28°c. c. megacephala developmental mean rate from egg to adult was 20.4 ± 0.38 d. first-instar larvae emerge in 1.4 ...	2014	26309267
estimating the number of eggs in blow fly (diptera: calliphoridae) egg masses using photographic analysis.	little work has been done to quantify the number of eggs oviposited by blow flies (diptera: calliphoridae) in studies examining colonization behavior. egg counting methods currently available are time-consuming and destructive. this study used imagej software and analysis of covariance to relate the volume of egg masses to the number of eggs laid by three different blow fly species: lucilia sericata (meigen), phormia regina (meigen), and chrysomya rufifacies (macquart). egg mass volume, species, ...	2015	26335472
the importance of habitat in the ecology of decomposition on rabbit carcasses in malaysia: implications in forensic entomology.	the stages of decomposition and the faunal succession on rabbit carcasses in three different habitats, namely jungle, rural, and highland areas, were studied. three new zealand white rabbit (oryctolagus cuniculus) carcasses weighing ∼2 kg were sampled daily until the decomposition process was completed. representative specimens of adult flies, larvae, pupa, and mites were collected from the carcasses and processed in the laboratory. there were differences in decomposition rate and faunal success ...	2015	26336275
forensic entomology of high-rise buildings in malaysia: three case reports.	the distributions of flies are not only confined to ground level but can also be at higher altitudes. here, we report three forensic cases involving dipterans in high-rise buildings in kuala lumpur, malaysia. case 1 involved a corpse of adult female found at the top floor of a fifteen-story apartment. case 2 dealt with a body of a 75-year-old female discovered in a bedroom on the eleventh floor of an eighteen-story building, while case 3 was a 52-year-old male found in his fifth floor shop house ...	2015	26691258
patterns of oviposition and development of chrysomya megacephala (fabricius) (diptera: calliphoridae) and chrysomya rufifacies (macquart) (diptera: calliphoridae) on burned rabbit carcasses.	considering that crimes against animals such as illegal killing and cruelty have been alarmingly increasing and since burning is one of the common ways for disposing cadavers, ability to estimate minimum postmortem interval (pmi) using entomological data merits consideration. chrysomya megacephala and chrysomya rufifacies are common necrophagous species recovered from cadavers in many countries including malaysia. specific studies focusing on the oviposition and developmental patterns of both sp ...	2016	26779962
methods for external disinfection of blow fly (diptera: calliphoridae) eggs prior to use in wound debridement therapy.	maggot debridement therapy (mdt) is the use of the larval stage of flies (i.e., calliphoridae) to remove necrotic tissue and disinfect wounds. effective mdt requires aseptic technique to prevent the unintentional introduction of pathogenic bacteria into a wound to be debrided; yet the external surface of calliphoridae eggs is often heavily contaminated with bacteria. studies of external disinfection of dipteran eggs have been reported, but neither their efficacy nor effect on egg viability has b ...	2016	27041484
larval distribution and behavior of chrysomya rufifacies (macquart) (diptera: calliphoridae) relative to other species on florida black bear (carnivora: ursidae) decomposing carcasses.	larval interactions of dipteran species, blow flies in particular, were observed and documented daily over time and location on five black bear carcasses in gainesville, fl, usa, from june 2002 - september 2004. cochliomyia macellaria (fabricius) or chrysomya megacephala (fabricius) larvae were collected first, after which chrysomya rufifacies (macquart) oviposited on the carcasses in multiple locations (i.e., neck, anus, and exposed flesh) not inhabited already by the other blow fly larvae. wit ...	2014	27193400
larval development rates of chrysomya rufifacies macquart, 1842 (diptera: calliphoridae) within its native range in south-east asia.	chrysomya rufifacies represents an important indicator species in forensic entomology that is often used to estimate the minimum postmortem interval (pmimin) in crime scene investigation. however, developmental rates differ locally, so that estimates should be based on regionally generated development data. therefore, we determined the developmental rates of c. rufifacies within its native range in thailand under nine constant temperature regimes: 15, 18, 21, 24, 27, 30, 33, 36 and 39°c. develop ...	2016	27213919
distribution modeling of three screwworm species in the ecologically diverse landscape of north west pakistan.	north west pakistan (nwp) is characterized by four eco-zones: northern montane region, north western hills, submontane region and indus plains. present study identified 1037 cases of traumatic myiasis in the region during 2012-2015. screw worm larvae were classified as 12 species: chrysomya bezziana (villeneuve), chryomya megacephala (fabricius), chrysomya rufifacies (macquart), lucilia cuprina (wiedemann), lucilia sericata (meigen), lucilia illustris (meigen), lucilia porphyrina (walker), hemip ...	2016	27311386
dataset of traumatic myiasis observed for three dominant screw worm species in north west pakistan with first report of wohlfahrtia magnifica (schiner).	regional surveys were carried out in different parts of north west pakistan among domestic animals (n=57,921) including pets and livestock identifying cases of traumatic myiasis (n=1037). a total of four surveys focused general livestock population during eid ul adha (eid surveys; incidence=1.21%) while another four surveys (miscellaneous surveys; incidence=7.34%) targeted animal population brought to veterinary hospitals and dispensaries. timeframe spanned four years from 2012 to 2015. maggots ...	2016	27570812
fluorescence imaging of posterior spiracles from second and third instars of forensically important chrysomya rufifacies (diptera: calliphoridae).	entomological protocols for aging blowfly (diptera: calliphoridae) larvae to estimate the time of colonization (toc) are commonly used to assist in death investigations. while the methodologies for analyzing fly larvae differ, most rely on light microscopy, genetic analysis, or, more rarely, electron microscopy. this pilot study sought to improve resolution of larval stage in the forensically important blowfly chrysomya rufifacies using high-content fluorescence microscopy and biochemical measur ...	2016	27706817
reflectance-based determination of age and species of blowfly puparia.	forensic entomology is primarily concerned with the estimation of time since death and involves determination of the age of immature insects colonising decomposing remains. accurate age determination of puparia is usually accomplished by dissection, which means destructive sampling of evidence. as part of improving abilities to correctly identify species and developmental age, it is highly desirable to have available non-destructive methods. in this study, we acquired external hyperspectral imag ...	2017	27766412
development of the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae), at five constant temperatures.	chrysomya megacephala (fabricius) is a forensically important fly that is found throughout the tropics and subtropics. we calculated the accumulated development time and transition points for each life stage from eclosion to adult emergence at five constant temperatures: 15, 20, 25, 30, and 35 °c. for each transition, the 10th, 50th, and 90th percentiles were calculated with a logistic linear model. the mean transition times and % survivorship were determined directly from the raw laboratory dat ...	2017	27816915
predictable weathering of puparial hydrocarbons of necrophagous flies for determining the postmortem interval: a field experiment using chrysomya rufifacies.	preadult development of necrophagous flies is commonly recognized as an accurate method for estimating the minimum postmortem interval (pmimin). however, once the pmimin exceeds the duration of preadult development, the method is less accurate. recently, fly puparial hydrocarbons were found to significantly change with weathering time in the field, indicating their potential use for pmimin estimates. however, additional studies are required to demonstrate how the weathering varies among species. ...	2017	28058571
morphological features of regurgitate and defecatory stains deposited by five species of necrophagous flies are influenced by adult diets and body size.	the morphological characteristics of artifacts from five species of necrophagous flies were examined following feeding on several types of diets. four types of insect stains were produced by each species: regurgitate, defecatory, translocation, and tarsal tracks. regurgitate was the most frequent type deposited (70.9 ± 2.4%), followed by defecatory (19.8 ± 4.0%), tarsal tracks (8.6 ± 1.2%), and translocation (0.7 ± 0.1%). artifact shapes, sizes, and color were highly variable and species and die ...	2018	28230912
analysis of the predetermining effect of a sex realizer by ovary transplantations in the monogenic flychrysomya rufifacies.	in the heterozygousf'/f female-producing females of the strictly monogenic blowflychrysomya rufifacies the gene product of the dominant or epistatic female sex realizerf' which causes sexpredetermination is thought to be synthesized either by cells of the germ line (oocytes, nurse cells or oogonia) or by somatic cells and then transferred into the oocytes. to determine the possible site of synthesis, reciprocal transplantations were made of prepupal ovaries between female-producing (thelygenic; ...	1980	28305153
resource use by an introduced and native carrion flies.	the carrion fly chrysomya rufifacies has recently been introduced to north america. larvae of this species are facultative predators on other carrion larvae, and are known to reduce populations of the new world fly cochliomyia macellaria in the laboratory and in certain field situations. in order to identify conditions under which native taxa might avoid interaction with the invader, we examined broad patterns of resource use by capturing postfeeding larvae as they left a carcass. the calliphori ...	1994	28313964
variation in the time of colonization of broiler carcasses by carrion flies in nakhonsawan province, thailand.	carrion flies are the primary insects colonizing vertebrate carrion; however, limited information is available on the variation in the time of colonization (toc) as related to time of placement (top) and time of death (tod), particularly in thailand. three seasonal sets of nine broiler carcasses (euthanized and placed in field within 0.5 h after death) were placed in mesh enclosures within a disturbed deciduous dipterocarp forest at nakhonsawan province, upper-central thailand, for 3 d to determ ...	2017	28444209
nonconsumptive effects of predatory chrysomya rufifacies (diptera: calliphoridae) larval cues on larval cochliomyia macellaria (diptera: calliphoridae) growth and development.	forensic entomologists often rely on development data associated with a given species to estimate when it colonized human or other vertebrate remains. in most instances, these development studies are based on single species reared in isolation in the laboratory. this study examined the impact of excretions and secretions (es) associated with third-instar chrysomya rufifacies (macquart), a predator, on the development of its prey, cochliomyia macellaria (f.). not surprisingly, ch. rufifacies es d ...	2017	28541462
ultrastructure of male genitalia of blow flies (diptera: calliphoridae) of forensic importance.	male genitalia of blow flies (diptera: calliphoridae) are distinctive in their morphological features and are often used for species identification. the aim of this work was to investigate the male genitalia of blow flies of medical and forensic importance from thailand at the ultrastructural level, using scanning electron microscopy (sem). flies in two subfamilies were examined: chrysomyinae [chrysomya bezziana villeneuve, chrysomya chani kurahashi, chrysomya nigripes aubertin, chrysomya pingui ...	2017	29248415