Publications

temperature modulates the secretome of the phytopathogenic fungus lasiodiplodia theobromae.	environmental alterations modulate host-microorganism interactions. little is known about how climate changes can trigger pathogenic features on symbiont or mutualistic microorganisms. current climate models predict increased environmental temperatures. the exposing of phytopathogens to these changing conditions can have particularly relevant consequences for economically important species and for humans. the impact on pathogen/host interaction and the shift on their biogeographical range can in ...	2016	27536303
metabolomics-guided isolation of anti-trypanosomal metabolites from the endophytic fungus lasiodiplodia theobromae.	fungal endophytes offer diverse and unique secondary metabolites, making these organisms potential sources of promising drug leads. the application of high-resolution-liquid chromatography mass spectrometry and nuclear magnetic resonance-based metabolomics to fungal endophytes is practical in terms of dereplication studies and the mining of bioactive compounds. in this paper, we report the application of metabolomics in parallel with anti-trypanosomal assays to determine the ideal conditions for ...	2016	27760442
invasive fungal sinusitis by lasiodiplodia theobromae in an patient with aplastic anemia: an extremely rare case report and literature review.	human lasiodiplodia theobromae infection has not been reported frequently. we report the first case of invasive l. theobromae nasal and neck infection. a 66-year-old male visited our hospital with anemia and general weakness. he showed pancytopenia, and his bone marrow examination revealed markedly decreased hematopoietic cells. the patient was presumed to have iatrogenic aplastic anemia due to mushroom toxicity. he began treatment for multiple organ infections with broad-spectrum antibiotics an ...	2016	27650099
isolation, purification and characterization of a novel steroidal saponin cholestanol glucoside from lasiodiplodia theobromae that induces apoptosis in a549 cells.	search for novel anticancer lead molecules continues to be a major focus of cancer research due to the limitations of existing drugs such as lack of tumor selectivity, narrow therapeutic index and multidrug resistance of cancer types. natural molecules often possess better pharmacokinetic traits compared to synthetic molecules as they continually evolve by natural selection process to interact with biological macromolecules. microbial metabolites constitute nearly half of the pharmaceuticals in ...	2016	26338072
first report of dieback caused by lasiodiplodia theobromae in strawberry plants in korea.	dieback in strawberry (seolhyang cultivar) was first observed during the nursery season (june to september) in the nonsan area of korea in the years 2012 and 2013. initial disease symptoms included dieback on runners, as well as black rot on roots, followed by wilting and eventually blackened, necrotic discoloration in the crowns of daughter plants. a fungus isolated from the diseased roots, runners, and crowns is close to lasiodiplodia theobromae based on morphological characteristics. analysis ...	2016	28154491
comparative assessment of fungal augmentation treatments of a fine-textured and historically oil-contaminated soil.	the removal of aged hydrophobic contaminants from fine-textured soils is a challenging issue in remediation. the objective of this study was to compare the efficacy of augmentation treatments to that of biostimulation in terms of total aliphatic hydrocarbon (tah) and toxicity removal from a historically contaminated clay soil and to assess their impact on the resident microbial community. to this aim, pleurotus ostreatus, botryosphaeria rhodina and a combination of both were used as the inoculan ...	2016	27220102
optimized jasmonic acid production by lasiodiplodia theobromae reveals formation of valuable plant secondary metabolites.	jasmonic acid is a plant hormone that can be produced by the fungus lasiodiplodia theobromae via submerged fermentation. from a biotechnological perspective jasmonic acid is a valuable feedstock as its derivatives serve as important ingredients in different cosmetic products and in the future it may be used for pharmaceutical applications. the objective of this work was to improve the production of jasmonic acid by l. theobromae strain 2334. we observed that jasmonic acid formation is dependent ...	2016	27907207
global transcriptional analysis suggests lasiodiplodia theobromae pathogenicity factors involved in modulation of grapevine defensive response.	lasiodiplodia theobromae is a fungus of the botryosphaeriaceae that causes grapevine vascular disease, especially in regions with hot climates. fungi in this group often remain latent within their host and become virulent under abiotic stress. transcriptional regulation analysis of l. theobromae exposed to heat stress (hs) was first carried out in vitro in the presence of grapevine wood (gw) to identify potential pathogenicity genes that were later evaluated for in planta expression.	2016	27514986
chemopreventive effect and lack of genotoxicity and mutagenicity of the exopolysaccharide botryosphaeran on human lymphocytes.	carbohydrate biopolymers of fungal-origin are an important natural resource in the search for new bioagents with therapeutic and nutraceutical potential. in this study the mutagenic, genotoxic, antigenotoxic and antioxidant properties of the fungal exopolysaccharide botryosphaeran, a (1→3)(1→6)-β-d-glucan, from botryosphaeria rhodina mamb-05, was evaluated. the mutagenicity was assessed at five concentrations in salmonella typhimurium by the ames test. normal and tumor (jurkat cells) human t lym ...	2016	27387458
3-o-β-d-glucopyranosyltheobroxide from aerial parts of cowpea (vigna unguiculata).	theobroxide has been isolated from culture filtrates of lasiodiplodia theobromae as a potato tuber-inducing compound. in this study, the metabolism of theobroxide was investigated using cowpea as an experimental model and [2h3-7]theobroxide as a substrate for analyzing a metabolite, which revealed that theobroxide applied exogenously to the roots was converted into 3-o-β-d-glucopyranosyltheobroxide.	2016	27319148
data from mass spectrometry, nmr spectra, gc-ms of fatty acid esters produced by lasiodiplodia theobromae.	the data described herein is related to the article with the title "fatty acid esters produced by lasiodiplodia theobromae function as growth regulators in tobacco seedlings" c.c. uranga, j. beld, a. mrse, i. cordova-guerrero, m.d. burkart, r. hernandez-martinez (2016) [1]. data includes nuclear magnetic resonance spectroscopy and gc-ms data used for the identification and characterization of fatty acid esters produced by l. theobromae. gc-ms traces are also shown for incubations in defined subs ...	2016	27274528
quantitative real-time pcr normalization for gene expression studies in the plant pathogenic fungi lasiodiplodia theobromae.	lasiodiplodia theobromae is a highly virulent plant pathogen. it has been suggested that heat stress increases its virulence. the aim of this work was to evaluate, compare, and recommend normalization strategies for gene expression analysis of the fungus growing with grapevine wood under heat stress. using rt-qpcr-derived data, reference gene stability was evaluated through genorm, normfinder and bestkeeper applications. based on the geometric mean using the ranking position obtained for each in ...	2016	27237774
successful treatment of lasiodiplodia theobromae keratitis - assessing the role of voriconazole.	the aim of this paper is to report the successful management of the first case of lasiodiplodia theobromae keratitis in hong kong.	2017	27843433
banana peel culture as an indigenous medium for easy identification of late-sporulation human fungal pathogens.	fungi are increasing in incidence as human pathogens and newer and rarer species are continuously being encountered. identifying these species from growth on regular culture media may be challenging due to the absence of typical features. an indigenous and cheap medium, similar to the natural substrate of these fungi, was standardised in our laboratory as an aid to species identification in a conventional laboratory setting.	2017	27934823
analysis of phylogeny, distribution, and pathogenicity of botryosphaeriaceae species associated with gummosis of anacardium in brazil, with a new species of lasiodiplodia.	netto, m. s. b., lima, w. g., correia, k. c., da silva, c. f. b., thon, m., martins, r. b., miller, r. n. g., michereff, s. j., and câmara, m. p. s. 2016. analysis of phylogeny, distribution, and pathogenicity of botryosphaeriaceae species associated with gummosis of anacardium in brazil, with a new species of lasiodiplodia. we identified botryosphaeriaceae species associated with gummosis on anacardium in brazil. isolates were sampled and identified on the basis morphology and phylogeny, throug ...	2017	28317544
intracerebroventricular administration of the (1→6)-β-d-glucan (lasiodiplodan) in male rats prevents d-penicillamine-induced behavioral alterations and lipoperoxidation in the cortex.	lasiodiplodan, an exocellular (1→6)-β-d-glucan of molecular weight >1.4 × 10(6) da produced by mmpi strain of lasiodiplodia theobromae (pat.) griffon & maubl. (brotyosphaeriaceae) is known to exhibit anti-proliferative activity on breast cancer cells (mcf-7), anticoagulant activity when sulfonylated, and reduction in transaminase activity when administered in rats.	2017	28274189
in vitro protective effects of botryosphaeran, a (1→3;1→6)-β-d-glucan, against mutagens in normal and tumor rodent cells.	botryosphaeran (bot) is an exocellular β-d-glucan (carbohydrate biopolymer) of the (1→3;1→6)-linked type produced by botryosphaeria rhodina mamb-05. the cytotoxic, mutagenic, genotoxic, and protective effects of this substance were evaluated in chinese hamster lung fibroblasts (v79) and rat hepatocarcinoma cells (htc) by the micronucleus test (mn) and the comet assay. bot was not genotoxic in either cell line; it decreased the clastogenic effects of doxorubicin, h2o2, and benzo[a]pyrene. these r ...	2017	28137365
protective effects of the exopolysaccharide lasiodiplodan against dna damage and inflammation induced by doxorubicin in rats: cytogenetic and gene expression assays.	the lasiodiplodan (ls) is a β-(1→6)-d-glucan produced by the fungus lasiodiplodia theobromae and some of the biological activities of ls were reported as hypoglycemic, anticoagulant, anti-proliferative and anticancer action; however, its effects on dna instability and modulation of gene expression are still unclear. aims of study were investigate the genotoxic effects of lasiodiplodan, and its protective activity against dna damage induced by doxorubicin (dxr) and its impact on the expression of ...	2017	27181935
agarwood formation induced by fermentation liquid of lasiodiplodia theobromae, the dominating fungus in wounded wood of aquilaria sinensis.	agarwood is broadly used in incense and medicine. traditionally, agarwood formation is induced by wounding the trunks and branches of some species of aquilaria spp., including a. sinensis. as recently evidenced, some fungi or their fermentation liquid may have the potential of inducing agarwood formation. the present study aimed to analyze the fungi isolated from an agarwood-producing a. sinensis tree and subsequently identify the fungi capable of promoting agarwood formation. we identified a to ...	2017	28224223
phylogeny and pathogenicity of lasiodiplodia species associated with dieback of mango in peru.	mango, which is an important tropical fruit crop in the region of piura (peru), is known to be prone to a range of diseases caused by lasiodiplodia spp. the aim of this study was to evaluate the incidence and prevalence of mango dieback in the region of piura, and to identify the species of lasiodiplodia associated with the disease and evaluate their pathogenicity towards mango. mango dieback was present in all orchards surveyed but incidence varied with location. identification of fungal isolat ...	2017	28317545