Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| synthesis, spectroscopic and biological aspects of iron(ii) complexes. | five novel coordinated complexes of iron(ii) with ciprofloxacin and neutral bidentate ligands have been prepared and characterized using elemental analyses, magnetic measurements, ir spectra, uv-vis spectral, thermogravimetric analyses, 1h-nmr and 13c-nmr. the antimicrobial activity of the individual ligands, metal salt and metal complexes with respect to bacillus subtilis, escherichia coli, bacillus cereus, staphylococcus aureus, salmonella typhi, serratia marcescens, aspergillus niger, aspergi ... | 2007 | 17847716 |
| lasiodiplodia theobromae pneumonia in a liver transplant recipient. | we report a case of lasiodiplodia theobromae pneumonia in a patient who died 14 days after cadaveric-liver transplantation. his condition was complicated by enterococcus faecium peritonitis. direct microscopy analysis of the bronchoalveolar lavage specimens showed septate hyphae. a dematiaceous mold was recovered and identified as l. theobromae by microscopic morphology and ef1alpha gene sequencing. | 2008 | 18003802 |
| anticlastogenic activity exhibited by botryosphaeran, a new exopolysaccharide produced by botryosphaeria rhodina mamb-05. | biopolymers such as exopolysaccharides (eps) are produced by microbial species and possess unusual properties known to modify biological responses, among them are antimutagenicity and immunomodulation. botryosphaeran, a newly described fungal (1-->3; 1-->6)-beta-d-glucan produced by botryosphaeria rhodina mamb-05, was administered by gavage to mice at three doses (7.5, 15 and 30mg/kgb.w.per day) over 15 days, and found to be non-genotoxic by the micronucleus test in peripheral blood and bone mar ... | 2008 | 18022685 |
| influence of nutrients on enhancing laccase production by botryosphaeria rhodina mamb-05. | the physiological requirements needed to enhance the production of laccases by the ascomycete botryosphaeria rhodina mamb-05 in submerged cultivation were examined under non-induced and induced (veratryl alcohol, va) conditions. under non-induced conditions (-va), the initial ph, c:n ratio, and inorganic n source did not influence laccase production, in contrast to tween 80, soybean oil, and copper, which significantly increased laccase production, and proline and urea, which suppressed laccase ... | 2007 | 18075999 |
| production of epoxide hydrolases in batch fermentations of botryosphaeria rhodina. | the filamentous fungus botryosphaeria rhodina (atcc 9055) was investigated related to its ability for epoxide hydrolase (eh) production. epoxide hydrolase activity is located at two different sites of the cells. the larger part is present in the cytosol (70%), while the smaller part is associated to membranes (30%). in media optimization experiments, an activity of 3.5 u/gdw for aromatic epoxide hydrolysis of para-nitro-styrene oxide (pnso) could be obtained. activity increased by 30% when pnso ... | 2008 | 18210174 |
| structural characterization of the cell wall d-glucans isolated from the mycelium of botryosphaeria rhodina mamb-05. | three d-glucans were isolated from the mycelium of the fungus botryosphaeria rhodina mamb-05 by sequential extraction with hot-water and hot aqueous koh (2% w/v) followed by ethanol precipitation. following their purification by gel permeation chromatography on sepharose cl-4b, the structural characteristics of the d-glucans were determined by ft-ir and 13c nmr spectroscopy and, after methylation, by gc-ms. the hot-water extract produced a fraction designated q1a that was a beta-(1-->6)-d-glucan ... | 2008 | 18237722 |
| iron(iii) complexes: preparation, characterization, antibacterial activity and dna-binding. | iron(iii) have been combined to well known quinolones (ciprofloxacin) and some schiff bases with the help of coordination approach. characterization of these compounds have been done using elemental analysis, magnetic measurements, thermogravimetric analysis, ir, uv-vis, (1)h nmr and (13)c nmr spectral investigation. analytical studies suggest that the iron(iii)-quinolone complexes assume a six-coordinated dimeric distorted octahedral geometry. all the compounds show a good antibacterial activit ... | 2008 | 18343909 |
| response surface methodology to optimize the nutritional parameters for enhanced production of jasmonic acid by lasiodiplodia theobromae. | to find out the cumulative effect of the nutritional parameters and to enhance the production of jasmonic acid (ja) in static fermentation by lasiodiplodia theobromae using response surface methodology (rsm). | 2008 | 18397253 |
| separation and quantitation of jasmonic acid using hptlc. | a rapid, simple, and stringent protocol for the detection and quantitation of jasmonic acid (ja) is designed using high-performance thin-layer chromatography. acidified culture filtrate of lasiodiplodia theobromae is extracted with an equal volume of ethyl acetate and spotted on silica gel 60 f(254) foil using linomat-5 spray-on applicator. standard ja is also spotted either internally or adjacent to the sample, and the foils are developed with isopropanol-ammonia-water [10:1:1 (v/v)] as the mob ... | 2008 | 18402723 |
| three exopolysaccharides of the beta-(1-->6)-d-glucan type and a beta-(1-->3;1-->6)-d-glucan produced by strains of botryosphaeria rhodina isolated from rotting tropical fruit. | four exopolysaccharides (eps) obtained from botryosphaeria rhodina strains isolated from rotting tropical fruit (graviola, mango, pinha, and orange) grown on sucrose were purified on sepharose cl-4b. total acid hydrolysis of each eps yielded only glucose. data from methylation analysis and (13)c nmr spectroscopy indicated that the eps from the graviola isolate consisted of a main chain of glucopyranosyl (1-->3) linkages substituted at o-6 as shown in the putative structure below: [carbohydrate s ... | 2008 | 18639868 |
| biocontrol efficacy of bacillus subtilis strains isolated from cow dung against postharvest yam (dioscorea rotundata l.) pathogens. | the biocontrol potential of bacillus subtilis isolated from cow dung microflora was investigated in vitro and in vivo against two postharvest yam pathogenic fungi, fusarium oxysporum and botryodiplodia theobromae. b. subtilis strains inhibited the growth of f. oxysporum and b. theobromae in vitro in liquid medium in the range of 49.3-56.6% and in solid medium in the range of 31.0-36.0%, in comparison to the corresponding growth of fungi without bacterial inoculation. the interaction between b. s ... | 2008 | 18663522 |
| new potato micro-tuber-inducing cyclohexene compounds related to theobroxide from lasiodiplodia theobromae. | two new cyclohexene compounds related to theobroxide (3) were isolated from the mycelia of lasiodiplodia theobromae ocs71. the structures of these compounds were determined to be (4s,5s)-4,5-dihydroxy-2-methyl-cyclohex-2-enone (1) and (3as,4r,5s,7ar)-4,5-dihydroxy-7-methyl-3a,4,5,7a-tetrahydrobenzo[1,3]dioxol-2-one (2) by means of spectroscopic analyses and chemical correlation to 3. compound 2 was shown to take up the carbonate ion to form a carbonic acid ester non-enzymatically. the compounds ... | 2008 | 18685216 |
| [studies on the effects of carbon:nitrogen ratio, inoculum type and yeast extract addition on jasmonic acid production by botryodiplodia theobromae pat. strain rc1]. | jasmonic acid is a native plant growth regulator produced by algae, microorganisms and higher plants. this regulator is involved in the activation of defence mechanisms against pathogens and wounding in plants. studies concerning the effects of carbon: nitrogen ratio (c/nr: 17, 35 and 70), type of inoculum (spores or mycelium) and the yeast extract addition in the media on jasmonic acid production by botryodiplodia theobromae were evaluated. jasmonic acid production was stimulated at the carbon: ... | 2008 | 18785793 |
| fungal diversity on fallen leaves of ficus in northern thailand. | fallen leaves of ficus altissima, f. virens, f. benjamina, f. fistulosa and f. semicordata, were collected in chiang mai province in northern thailand and examined for fungi. eighty taxa were identified, comprising 56 anamorphic taxa, 23 ascomycetes and 1 basidiomycete. common fungal species occurring on five host species with high frequency of occurrence were beltraniella nilgirica, lasiodiplodia theobromae, ophioceras leptosporum, periconia byssoides and septonema harknessi. colletotrichum and ... | 2008 | 18837113 |
| fusarium species from the cassava root rot complex in west africa. | abstract fusarium species are a significant component of the set of fungi associated with cassava root rot. yield losses due to root rot average 0.5 to 1 ton/ha but losses >3 ton/ha, an equivalent of 15 to 20% yield, often occur. this paper reviews previous work on cassava root rot and summarizes a few recent studies on fusarium species associated with the disease. our studies in cameroon showed that 30% of rotted tubers were infected by fusarium spp. 12 months after planting and represented 25% ... | 2006 | 18943189 |
| influence of culture media and environmental factors on mycelial growth and sporulation of lasiodiplodia theobromae (pat.) griffon and maubl. | lasiodiplodia theobromae, a common tea (camellia sinensis) pathogen, usually does not sporulate or sporulates poorly in common media, which makes spore production difficult. in this study the effects of culture media, carbon source, nitrogen source, temperature, ph and light on mycelial growth and sporulation were evaluated. among several carbon sources tested, glucose and sucrose were found superior for growth. potassium nitrate supplemented media showed maximum growth amongst the tested inorga ... | 2008 | 18972700 |
| fungal keratitis in far north queensland, australia. | aboriginals and torres strait islanders form a significant proportion of the population in far north queensland and are at increased risk for many eye conditions. this study describes the pattern of fungal keratitis seen at cairns base hospital, far north queensland, australia. | 2008 | 19128375 |
| on the diversity of the laccase gene: a phylogenetic perspective from botryosphaeria rhodina (ascomycota: fungi) and other related taxa. | the present study is the first describing the sequencing of a fragment of the copper-oxidase domain of a laccase gene in the family botryosphaeriaceae. the aim of this work was to assess the degree of genetic and evolutionary relationships of a laccase gene from botryosphaeria rhodina mamb-05 with other ascomycete and basidiomycete laccase genes. the 193-amino acid sequences of the copper-oxidase domain from several different fungi, insects, a plant, and a bacterial species were retrieved from g ... | 2009 | 19160039 |
| biosynthesis of resorcylic acid lactone lasiodiplodin in lasiodiplodia theobromae. | the biosynthesis of lasiodiplodin (1) and its (5s)-5-hydroxylated derivative (2) were investigated by the administration of (13)c-labeled acetates to lasiodiplodia theobromae. the labeling patterns of biosynthetically (13)c-labeled 1 and 2 were determined by (13)c-nmr and inadequate spectra, demonstrating the octaketide origins of 1 and 2. taking into account the biosynthetic study of resorcylic acid lactones, the involvement of highly reduced acyl intermediates in the biosynthesis of lasiodiplo ... | 2009 | 19420710 |
| sulfonation and anticoagulant activity of botryosphaeran from botryosphaeria rhodina mamb-05 grown on fructose. | botryosphaeran (eps(fru)), an exopolysaccharide of the beta-(1-->3,1-->6)-d-glucan type with 31% branching at c-6, is produced by the fungus botryosphaeria rhodina mamb-05 when grown on fructose as carbon source. botryosphaeran was derivatized by sulfonation to induce anticoagulant activity. the effectiveness of the sulfonation reaction by chlorosulfonic acid in pyridine was monitored by the degree of substitution and ft-ir analysis of the sulfonated eps(fru) (once sulfonated, eps(frusulf); and ... | 2009 | 19549543 |
| isolation and structural elucidation of a new cyclohexenone compound from lasiodiplodia theobromae. | a new cyclohexenone compound was isolated as a mixture of enantiomers from a culture filtrate of lasiodiplodia theobromae. the relative structure was determined to be 4,5-dihydroxy-3-methyl-cyclohex-2-enone on the basis of ms, (1)h-nmr, and (13)c-nmr spectroscopic analyses, including 2d-nmr experiments. resolution of the enantiomers was conducted by a coupling reaction with (s)-mtpa-cl followed by hplc separation. | 2009 | 19661717 |
| anticancer activity of fungal taxol derived from botryodiplodia theobromae pat., an endophytic fungus, against 7, 12 dimethyl benz(a)anthracene (dmba)-induced mammary gland carcinogenesis in sprague dawley rats. | breast cancer is the second most prevalent cancer worldwide and their incidence increases gradually. taxol (paclitaxel), a potent anticancer drug, is naturally isolated from the bark of the pacific yew. taxol is widely used in the treatment of ovarian, lung and breast cancer. the increased demand for taxol, coupled with its limited availability from the protected pacific yew, has had researchers scrambling for alternate sources. the purpose of the present study is to investigate chemopreventive ... | 2010 | 19762199 |
| methyl angolensate changes in khaya ivorensis after fungal infection. | khaya ivorensis with and without symptoms of stem and branch cankers, caused by botryosphaeria rhodina were examined in order to determine whether the secondary metabolites in this plant were associated with a chemical defense response. this study provides evidence that the limonoid methyl angolensate (ma) is present at higher concentrations in k. ivorensis with symptoms of stem cankers rather than in the plants without symptoms. a rapid, sensitive and selective hplc-esi-ms/ms method (using sele ... | 2009 | 19765786 |
| biosynthesis of resorcylic acid lactone (5s)-5-hydroxylasiodiplodin in lasiodiplodia theobromae. | an administration study of (2)h-labeled precursors showed that the 9-hydroxydecanoyl unit, the acyl intermediate of lasiodiplodin (1), was also the intermediate of (5s)-5-hydroxylasiodiplodin (2) in lasiodiplodia theobromae. the incorporation of [o-methyl-(2)h(3)]-lasiodiplodin (6) into 2 indicated that hydroxylation at c-5 occurred after cyclization. | 2009 | 19897901 |
| botryorhodines a-d, antifungal and cytotoxic depsidones from botryosphaeria rhodina, an endophyte of the medicinal plant bidens pilosa. | an endophytic fungus (botryosphaeria rhodina) was isolated from the stems of the medicinal plant bidens pilosa (asteraceae) that is known for its anti-inflammatory, antiseptic and antifungal effects. the ethyl acetate extract of the fungal isolate exhibits significant antifungal activity as well as potent cytotoxic and antiproliferative effects against several cancer cell lines. activity-guided fractionation resulted in the isolation of a complex of four depsidones, botryorhodines a-d and the au ... | 2010 | 19913264 |
| antifungal activities of ethanolic extract from jatropha curcas seed cake. | phorbol ester extraction was carried out from jatropha curcas seed cake, a by-product from the bio-diesel fuel industry. four repeated extractions from 5 g j. curcas seed cake using 15 ml of 90% (v/v) ethanol and a shaking speed of 150 rev/min gave the highest yield of phosbol esters. the ethanolic extract of j. curcas seed cake showed antifungal activities against important phytofungal pathogens: fusarium oxysporum, pythium aphanidermatum, lasiodiplodia theobromae, curvularia lunata, fusarium s ... | 2010 | 20208435 |
| maxillary sinusitis caused by lasiodiplodia theobromae. | lasiodiplodia (monotypic) comprises a very small proportion of the fungal biota. it is a common plant pathogen in tropical and subtropical regions. clinical reports on its association with onychomycosis, corneal ulcer and phaeohyphomycosis are available. however, lasiodiplodia theobromae causing fungal sinusitis has not been reported. we present here a case of fungal sinusitis in a 30-year-old woman, who came to the ent opd (out patient department) with complaints of intermittent bleeding and na ... | 2010 | 20404468 |
| morphological, pathological and molecular variability in botryodiplodia theobromae (botryosphaeriaceae) isolates associated with die-back and bark canker of pear trees in punjab, india. | thirteen isolates of botryodiplodia theobromae collected from pear varieties grown in various regions of punjab were studied for morphological, pathological and molecular characterization. the mycelial growth of b. theobromae isolates was classified as fluffy or depressed, uniform to irregular and cottony white turning to black. colony growth rate varied from 19.1 to 24.9 mm per day. pycnidia were produced either on the edge, centered or scattered on petri dishes after 20 to 34 days of incubatio ... | 2010 | 20603807 |
| medicinal mushroom ganoderma lucidum as a potent elicitor in production of t-resveratrol and t-piceatannol in peanut calluses. | phytoalexins t-resveratrol and t-piceatannol, the well-known health-promoting active components in plants, are secondary metabolites generated upon biotic or abiotic stresses. we have reported uv-irradiated peanut callus is a potent means to produce these compounds (j. agric. food chem. 2005, 53, 3877). in this work, the effects of fungi and chemical elicitors on induction of t-resveratrol and t-piceatannol were examined. results showed the investigated fungi botryodiplodia theobromae and reishi ... | 2010 | 20687553 |
| fungi associated with storage rots of cocoyams (colocasia spp.) in nsukka, nigeria. | cocoyam (colocasia spp.) corms and cormels showing spoilage symptoms were collected from many stores in nsukka locality and examined for rot and associated fungal pathogens. aspergillus niger, botryodiplodia theobromae, corticium rolfsii, geotrichum candidum, fusarium oxysporum, and f. solani were recovered from rotten cocoyams. the representative isolates of these species caused cocoyam rot in pathogenicity tests. the rot due to a. niger, b. theobromae and c. rolfsii was extensive resulting in ... | 1996 | 20882465 |
| biosynthesis of jasmonic acid in a plant pathogenic fungus, lasiodiplodia theobromae. | jasmonic acid (ja) is a plant hormone that plays an important role in a wide variety of plant physiological processes. the plant pathogenic fungus, lasiodiplodia theobromae also produces ja; however, its biosynthesis in this fungus has yet to be explored. administration of [1-(13)c] and [2-(13)c] naoac into l. theobromae established that ja in this fungus originates from a fatty acid synthetic pathway. the methyl ester of 12-oxo-phytodienoic acid (opda) was detected in the culture extracts of l. ... | 2010 | 20952041 |
| evaluation of lactoperoxidase system treatment to reduce anthracnose, stem-end rot, and bacterial black spot development during storage of mangoes. | the lactoperoxidase system (lps) was evaluated for the prevention of postharvest diseases caused by xanthomonas campestris, botryodiplodia theobromae, and colletotrichum gloeosporioides in 'keitt' and 'kent' mangoes. the lps treatment significantly reduced the disease development on both cultivars after storage at 12 degrees c for 2 weeks, which was followed by a ripening at 25 degrees c. the lps treatment did not alter the sensory quality of mango fruits (color, firmness, titrable acidity, and ... | 2005 | 21132977 |
| toxicological and immunomodulatory assessments of botryosphaeran (β-glucan) produced by botryosphaeria rhodina rcyu 30101. | toxicological and immunomodulatory activities of botryosphaeran (br), a newly emerged β-glucan that comprises a β-(1 → 3) backbone and β-(1 → 6) branched glucose residues were assessed. br was 1.82 × 10(6) da (m.w.) estimated by reversely-linear equation constructed by regression of logarithms of standard polysaccharides and their retention times of gel permeation chromatography. sprague-dawley rats were daily gavage-administered with br at doses of 0, 1.25, 12.5, and 125 mg/kg body weight (bw) ... | 2010 | 21185904 |
| botryosphaeriaceae associated with pterocarpus angolensis (kiaat) in south africa. | there have been several recent reports of pterocarpus angolensis (kiaat) trees dying in south africa, zambia and zimbabwe, where this tree is used in traditional medicine and is a valuable source of timber for woodcarving and furniture. a survey of material from diseased p. angolensis trees in south africa yielded isolates of the botryosphaeriaceae, an important fungal family known to cause a number of tree diseases. the aim of this study was to identify these botryosphaeriaceae and to determine ... | 2010 | 21186325 |
| colonization of cashew plants by lasiodiplodia theobromae: microscopical features. | lasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in brazil. in an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. optical microscopy analysis re ... | 2010 | 21194959 |
| Thermal and rheological properties of a family of botryosphaerans produced by Botryosphaeria rhodina MAMB-05. | Differential scanning calorimetry (DSC), thermogravimetry (TG) and Fourier-transform infra-red spectroscopy (FT-IR) analyses were performed to investigate changes in the physico-chemical properties of botryosphaerans, a family of exopolysaccharides (EPS) produced by the fungus Botryosphaeria rhodina MAMB-05 grown on glucose (EPS(GLC)), sucrose (EPS(SUC)) and fructose (EPS(FRU)). A slight endothermic transition and small mass loss attributable to the removal of water of hydration were observed in ... | 2011 | 21892127 |
| Chemical modification of botryosphaeran: structural characterization and anticoagulant activity of a water-soluble sulfonated (1-->3)(1-->6)-ß-D-glucan. | The exopolysaccharide botryosphaeran (EPS(GLC); a (1--> 3)(1-->6)-ß-D-glucan from Botryosphaeria rhodina MAMB- 05) was sulfonated to produce a water-soluble fraction (EPS(GLC)-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction (EPSGLC-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where ... | 2011 | 22031027 |
| linolenate 9r-dioxygenase and allene oxide synthase activities of lasiodiplodia theobromae. | jasmonic acid (ja) is synthesized from linolenic acid (18:3n-3) by sequential action of 13-lipoxygenase, allene oxide synthase (aos), and allene oxide cyclase. the fungus lasiodiplodia theobromae can produce large amounts of ja and was recently reported to form the ja precursor 12-oxophytodienoic acid. the objective of our study was to characterize the fatty acid dioxygenase activities of this fungus. two strains of l. theobromae with low ja secretion (~0.2 mg/l medium) oxygenated 18:3n-3 to 5,8 ... | 2012 | 22048860 |
| A new antifungal coumarin from Clausena excavata. | A new ?-lactone coumarin, named as excavarin-A, showing antifungal activity was isolated from the leaves of Clausena excavata by bioassay guided fractionation method. The structure was elucidated by spectroscopic data analysis and identified as 7((2E)-4(4,5-dihydro-3-methylene-2-oxo-5-furanyl)-3-methylbut-2-enyloxy) coumarin. Minimum inhibitory concentration (MIC) was determined against fifteen fungal strains pathogenic against plants and human. The least MIC was recorded against the human patho ... | 2012 | 22088496 |
| [isolation of highly-effective benzo[a]pyrene degrading strain and its degradation capacity]. | one new mycete, which could degrade high concentration (up to 100 mg/l) of benzo[a]pyrene (bap) in liquid, was isolated from contaminated soil of beijing coking plant by gradually increasing the concentration of bap in mineral salt medium (msm) in order to get new microorganism species for remediation of bap contamination. the strain was identified as lasiodiplodia theobromae, and its biodegradation ability in liquid was further investigated. the results showed that l. theobromae could utilize b ... | 2011 | 22165241 |
| biotransformations of 6',7'-dihydroxybergamottin and 6',7'-epoxybergamottin by the citrus-pathogenic fungi diminish cytochrome p450 3a4 inhibitory activity. | penicillium digitatum, as well as five other citrus pathogenic species, (penicillium ulaiense link, geotrichum citri link, botrytis cinerea p. micheli ex pers., lasiodiplodia theobromae (pat.) griffon & maubl., and phomopsis citri (teleomorph diaporthe citri)) were observed to convert 6',7'-epoxybergamottin (1) into 6',7'-dihydroxybergamottin (2), bergaptol (3), and an opened lactone ring metabolite 6,7-furano-5-(6',7'-dihydroxy geranyloxy)-2-hydroxy-hydrocoumaric acid (4). metabolism of 2 by th ... | 2012 | 22342630 |
| high gene flow and outcrossing within populations of two cryptic fungal pathogens on a native and non-native host in cameroon. | in this study, we determined the genetic diversity of 126 isolates representing both lasiodiplodia theobromae and lasiodiplodia pseudotheobromae, collected from theobroma cacao and terminalia spp. in cameroon, using simple sequence repeat (ssr) markers. ssr alleles showed clear genetic distinction between l. theobromae and l. pseudotheobromae, supporting their earlier separation as sister species. both l. theobromae and l. pseudotheobromae populations from cameroon had high levels of gene divers ... | 2011 | 22385617 |
| improved cellulase production by botryosphaeria rhodina from opefb at low level moisture condition through statistical optimization. | the response surface method was applied in this study to improve cellulase production from oil palm empty fruit bunch (opefb) by botryosphaeria rhodina. an experimental design based on a two-level factorial was employed to screen the significant environmental factors for cellulase production. the locally isolated fungus botryosphaeria rhodina was cultivated on opefb under solid-state fermentation (ssf). from the analysis of variance (anova), the initial moisture content, amount of substrate, and ... | 2012 | 22394064 |
| lasiodiplodan, an exocellular (1→6)-β-d: -glucan from lasiodiplodia theobromae mmpi: production on glucose, fermentation kinetics, rheology and anti-proliferative activity. | lasiodiplodan, an exopolysaccharide of the (1→6)-β-d: -glucan type, is produced by lasiodiplodia theobromae mmpi when grown under submerged culture on glucose. the objective of this study was to evaluate lasiodiplodan production by examining the effects of carbon (glucose, fructose, maltose, sucrose) and nitrogen sources (kno(3), (nh(4))(2)so(4), urea, yeast extract, peptone), its production in shake flasks compared to a stirred-tank bioreactor, and to study the rheology of lasiodiplodan, and la ... | 2012 | 22399240 |
| season and tissue type affect fungal endophyte communities of the indian medicinal plant tinospora cordifolia more strongly than geographic location. | a total of 1,151 endophytic fungal isolates representing 29 taxa were isolated from symptom-less, surface-sterilized segments of stem, leaf, petiole, and root of tinospora cordifolia which had been collected at three locations differing in air pollution in india (ramnagar, banaras hindu university, maruadih) during three seasons (summer, monsoon, winter). endophytes were most abundant in leaf tissues (29.38% of all isolates), followed by stem (18.16%), petiole (10.11%), and root segments (6.27%) ... | 2012 | 22430503 |
| lasiodiplodia theobromae keratitis: a case report and review of literature. | a case report and review of literature is reported of a rare case of fungal keratitis from eastern india. a 32-year-old woman with a history of vegetative trauma presented with keratitis in left eye. microbiological examination of corneal scraping showed refractile hyphae with aseptate branching filaments and black pigmented colonies on multiple solid agar medium. organism was identified from culture using d1/d2 region of lsu (large sub unit: 28s rdna)-based molecular technique. pcr amplified a ... | 2012 | 22544631 |
| hypoglycemic and hypocholesterolemic effects of botryosphaeran from botryosphaeria rhodina mamb-05 in diabetes-induced and hyperlipidemia conditions in rats. | botryosphaeran, a water-soluble exopolysaccharide of the β-(1 → 3;1 → 6)-d-glucan type that has been isolated from the culture medium of botryosphaeria rhodina mamb-05 grown in submerged fermentation using glucose as the sole carbon source, was previously demonstrated to be non-genotoxic in peripheral blood and bone marrow, and exhibited strong anticlastogenic activity. in the present study, the effects of botryosphaeran were investigated in streptozotocin-induced diabetic rats as well as in hig ... | 2011 | 22783102 |
| diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes. | commercial oil-yielding seeds (castor, coconut, neem, peanut, pongamia, rubber and sesame) were collected from different places in the state of tamil nadu (india) from which 1279 endophytic fungi were isolated. the oil-bearing seeds exhibited rich fungal diversity. high shannon-index h' was observed with pongamia seeds (2.847) while a low index occurred for coconut kernel-associated mycoflora (1.018). maximum colonization frequency (%) was observed for lasiodiplodia theobromae (176). dominance i ... | 2011 | 22806781 |
| one-pot synthesis of novel isoindoline-1,3-dione derivatives bearing 1,2,4-triazole moiety and their preliminary biological evaluation. | a series of novel isoindoline-1,3-diones containing 1,2,4-triazole moiety were synthesized via a one-pot reaction. bioassay indicated that compounds 33, 35, 37 and 39 exhibited much higher activities against botryodiplodia theobromae than commercial fungicide triadimefon at the dosage of 150 mg/l. most interestingly, compounds 36, 37 and 45 displayed much stronger antitumor activities against four human cell lines than positive control fluorouracil. particularly, compound 37 had four-fold improv ... | 2012 | 22809558 |
| catalytic asymmetric synthesis of butane diacetal-protected (4s,5s)-dihydroxycyclohexen-1-one and use in natural product synthesis. | due to the lack of availability of unnatural (+)-quinic acid as a starting material, a 6-step synthesis of butane diacetal-protected (4s,5s)-dihydroxycyclohexen-1-one (formally derived from (+)-quinic acid) has been devised. the key catalytic asymmetric step involves a chiral co-salen-catalysed epoxide ring-opening reaction. (4s,5s)-dihydroxycyclohexen-1-one was utilised in the synthesis of two cyclohexenone natural products isolated from the mycelia of lasiodiplodia theobromae. | 2012 | 22930235 |
| toxicological assessment of β-(1-->6)-glucan (lasiodiplodan) in mice during a 28-day feeding study by gavage. | studies evaluating the toxicity caused by fungal exopolysaccharides of the β-(1-->6)-d-glucan type are rare. in this study, the toxicological effects of sub-chronic treatments with lasiodiplodan (β-(1-->6)-d-glucan from lasiodiplodia theobromae mmpi) were evaluated in mice through the assessment of biochemical, hematological, and histopathological alterations. thirty-two mice (16 male, 16 female) were used in this study divided in two groups; one group received lasiodiplodan (50 mg/kg body weigh ... | 2012 | 23208465 |
| [bioremediation of pahs contaminated soil from beijing coking plant by lasiodiplodia theobromae]. | bioremediation of pahs contaminated soil from beijing coking plant was performed using a novel fungal strain lasiodiplodia theobromae (l. theobromae). moreover, enhanced bioremediation of pahs contaminated soil was investigated in the presence of different concentrations of tween 80 and hydroxypropyl-beta-cyclodextrin (hpcd). the correlation of the dynamics of enzyme activities during remediation and the degradation of pahs was analyzed. the results showed that the degradation rate of pahs incre ... | 2012 | 23213912 |
| pcr-sscp-based reconstruction of the original fungal flora of heat-processed meat products. | food processing of spoiled meat is prohibited by law, since it is a deception and does not comply with food safety aspects. in general, spoilage of meat is mostly caused by bacteria. however, a high contamination level of fungi could be also found in some meat or meat products with certain preserving conditions. in case that unhygienic meat is used to produce heat processed products, the microorganisms will be deactivated by heat, so that they cannot be detected by a standard cultivation method. ... | 2013 | 23361099 |
| sulfonation and anticoagulant activity of fungal exocellular β-(1→6)-d-glucan (lasiodiplodan). | an exocellular β-(1→6)-d-glucan (lasiodiplodan) produced by a strain of lasiodiplodia theobromae (mmlr) grown on sucrose was derivatized by sulfonation to promote anticoagulant activity. the structural features of the sulfonated β-(1→6)-d-glucan were investigated by uv-vis, ft-ir and (13)c nmr spectroscopy, and the anticoagulant activity was investigated by the classical coagulation assays aptt, pt and tt using heparin as standard. the content of sulfur and degree of substitution of the sulfonat ... | 2012 | 23399236 |
| optimization of culture conditions for some identified fungal species and stability profile of α-galactosidase produced. | microbial α-galactosidase preparations have implications in medicine and in the modification of various agricultural products as well. in this paper, four isolated fungal strains such as al-3, wf-3, wp-4 and cl-4 from rhizospheric soil identified as penicillium glabrum (al-3), trichoderma evansii (wf-3), lasiodiplodia theobromae (wp-4) and penicillium flavus (cl-4) based on their morphology and microscopic examinations, are screened for their potential towards α-galactosidases production. the cu ... | 2013 | 23424684 |
| isolation, identification and antimicrobial activity of propolis-associated fungi. | propolis is a natural product widely known for its medicinal properties. in this work, fungi present on propolis samples were isolated, identified and tested for the production of antimicrobial metabolites. twenty-two fungal isolates were obtained, some of which were identified as alternaria alternata, aspergillus flavus, bipolaris hawaiiensis, fusarium merismoides, lasiodiplodia theobromae, penicillium citrinum, penicillium crustosum, penicillium janthinellum, penicillium purpurogenum, pestalot ... | 2013 | 23439233 |
| synthesis and structure-activity relationship of n-(cinnamyl) chitosan analogs as antimicrobial agents. | the current study focuses on the preparation of new n-(cinnamyl) chitosan derivatives as antimicrobial agents against nine types of crop-threatening pathogens. chitosan was reacted with a set of aromatic cinnamaldehyde analogs by reductive amination involving formation of the corresponding imines, followed by reduction with sodium borohydride to produce n-(cinnamyl) chitosan derivatives. the structural characterization was confirmed by (1)h and (13)c nmr spectroscopy and the degrees of substitut ... | 2013 | 23511055 |
| lasiodiplodia theobromae is a mycoparasite of a powdery mildew pathogen. | powdery mildews on over 40 plants in bangalore were screened during july-december of 2003~2008. isolates from mycoparasitised oidium caesalpiniacearum of bauhinia purpurea comprised lasiodiplodia theobromae, in addition to ampelomyces quisqualis. koch's postulates were satisfied to establish the mycoparasitism of l. theobromae. this is the first report that l. theobromae acts as a mycoparasite of a powdery mildew. | 2009 | 23983554 |
| [identification of pathogen of aquilaria sinensis dieback disease]. | the research focused on identification the pathogen of aquilaria sinensis dieback disease and confirmation the function of inducing agarwood formation by fungi. | 2013 | 24010282 |
| unusual causes of fungal rhinosinusitis: a study from a tertiary care centre in south india. | the frequency of mycotic infections of the nose and paranasal sinuses has been increasing over the past three decades. apart from the common causes of fungal rhinosinusitis such as aspergillus species and penicillium species, there have been reports of rare and unusual fungi isolated from india and other countries. | 2015 | 24064646 |
| keratitis due to the wood saprobic ascomycete, auerswaldia lignicola (family botryosphaeriaceae), in a carpenter in india. | keratitis due to auerswaldia lignicola in a 32-year-old indian male carpenter is described. at presentation, the patient reported persistent pain and tearing (left eye) in spite of topical antimicrobial therapy for more than 3 weeks. clinically, mycotic keratitis was suspected, and direct microscopy of corneal scrapings stained by lactophenol cotton blue and gram stains revealed broad septate hyphae. intensive topical antifungal therapy was then given for 15 days. the keratitis continued to prog ... | 2013 | 24158617 |
| carbohydrate metabolism changes in prunus persica gummosis infected with lasiodiplodia theobromae. | peach gummosis represents a significant global disease of stone fruit trees and a major disease in the south peach production area of the yangtze river of china. in this study, the carbohydrate composition of peach shoots during infection by lasiodiplodia theobromae was examined. the expression of genes related to metabolic enzymes was also investigated. control wounded and noninoculated tissue, lesion tissue, and wounded and inoculated surrounding lesion tissue of peach shoots were analyzed. so ... | 2014 | 24283537 |
| the botryosphaeriaceae: genera and species known from culture. | in this paper we give an account of the genera and species in the botryosphaeriaceae. we consider morphological characters alone as inadequate to define genera or identify species, given the confusion it has repeatedly introduced in the past, their variation during development, and inevitable overlap as representation grows. thus it seems likely that all of the older taxa linked to the botryosphaeriaceae, and for which cultures or dna sequence data are not available, cannot be linked to the spec ... | 2013 | 24302790 |
| simultaneous quantification of phytohormones in fermentation extracts of botryodiplodia theobromae by liquid chromatography-electrospray tandem mass spectrometry. | fermentation broth and biomass from three strains of botryodiplodia theobromae were characterized by high performance liquid chromatography-electrospray tandem mass spectrometry (hplc-esi-ms/ms) method, in order to quantify different phytohormones and to identify amino acid conjugates of jasmonic acid (ja) present in fermentation broths. a liquid-liquid extraction with ethyl acetate was used as sample preparation. the separation was carried out on a c18 reversed-phase hplc column followed by ana ... | 2014 | 24510403 |
| insights into the biosynthesis of 12-membered resorcylic acid lactones from heterologous production in saccharomyces cerevisiae. | the phytotoxic fungal polyketides lasiodiplodin and resorcylide inhibit human blood coagulation factor xiiia, mineralocorticoid receptors, and prostaglandin biosynthesis. these secondary metabolites belong to the 12-membered resorcylic acid lactone (ral12) subclass of the benzenediol lactone (bdl) family. identification of genomic loci for the biosynthesis of lasiodiplodin from lasiodiplodia theobromae and resorcylide from acremonium zeae revealed collaborating iterative polyketide synthase (ipk ... | 2014 | 24597618 |
| agrobacterium tumefaciens-mediated transformation of lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants. | lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. this paper describes an efficient agrobacterium tumefaciens-mediated transformation (atmt) system for the successful delivery of t-dna, transferring the genes of green fluorescent protein (gfp) and hygromycin b phosphotransferase (hph) to l. theobromae. when the fungal pycnidiospores were co-cultured with a. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fun ... | 2014 | 24634294 |
| effect of culture media and environmental factors on mycelial growth and pycnidial production of lasiodiplodia theobromae in physic nut (jatropha curcas). | physic nut (jatropha curcas) is an important commercial bio-diesel plant species and is being advocated for development of waste and dry land. the collar and root rot caused by lasiodiplodia theobromae is an important soil borne disease which causes considerable yield loss in this crop. in this study, the effects of culture media, temperature, photoperiod, carbon and nitrogen sources and ph on mycelial growth and pycnidial production were evaluated. among the growth media tested, potato dextrose ... | 2013 | 24640243 |
| [study of production of sesquiterpenes of aquilaria senensis stimulated by lasiodiplodia theobromae]. | to investigate the mechanism of agarwood formation in aquilaria sinensis induced by lasiodiplodia theobromae, the fermentation liquor of l. theobromae was analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry (gc-ms). jas were detected in the fermentation liquor. the effect of the fermentation liquor on the abundance of sesquiterpenes in the callus of a. sinensis was analyzed by solid phase microextraction-gas chromatography-mass spectrometry (spme-gc-ms). and the fer ... | 2014 | 24761630 |
| succession patterns of fungi associated to wound-induced agarwood in wild aquilaria malaccensis revealed from quantitative pcr assay. | aquilaria malaccensis produces agarwood in response to wounding and fungal attack. however, information is limited regarding aquilaria's interaction with its diverse fungal community. in this study, time-related changes of three natural fungal colonizers in two wounded wild a. malaccensis were tracked, beginning a few hours after wounding up to 12 months. using species-specific primers derived from their nrits sequences in quantitative real-time pcr (qpcr), we quantified the amount of cunningham ... | 2014 | 24840100 |
| degradation of pahs in soil by lasiodiplodia theobromae and enhanced benzo[a]pyrene degradation by the addition of tween-80. | benzo[a]pyrene (bap), a five-ring polycyclic aromatic hydrocarbon (pah), which has carcinogenic potency, is highly recalcitrant and resistant to microbial degradation. a novel fungus, lasiodiplodia theobromae (l. theobromae), which can degrade bap as a sole carbon source in liquid, was isolated in our laboratory. to prompt the further application of l. theobromae in remediation of sites polluted by bap and other pahs, the present study was targeted toward the removal of bap and pahs from soil by ... | 2014 | 24878554 |
| dieback and sooty canker of ficus trees in egypt and its control. | this study was designed to throw lights on dieback and canker disease on urban trees of ficus sp. in egypt, its causal pathogens and disease control. diseased samples were collected from five locations. pathogenicity test was done on one year old of three different healthy seedlings of ficus trees (ficus benghalensis, ficu snitida and ficus hawaii). lasiodiplodia theobromae and phomopsis sp. were consistently isolated from infected tissues and were pathogenic. the fungicides antracol combi and t ... | 2014 | 24897790 |
| antimicrobial and inhibitory enzyme activity of n-(benzyl) and quaternary n-(benzyl) chitosan derivatives on plant pathogens. | chemical modification of a biopolymer chitosan by introducing quaternary ammonium moieties into the polymer backbone enhances its antimicrobial activity. in the present study, a series of quaternary n-(benzyl) chitosan derivatives were synthesized and characterized by (1)h-nmr, ft-ir and uv spectroscopic techniques. the antimicrobial activity against crop-threatening bacteria agrobacterium tumefaciens and erwinia carotovora and fungi botrytis cinerea, botryodiplodia theobromae, fusarium oxysporu ... | 2014 | 25037402 |
| screening of bacterial biocontrols against sapstain fungus (lasiodiplodia theobromae pat.) of rubberwood (hevea brasiliensis muell.arg.). | diverse bacterial biocontrol agents from various sources of aerobic composts against the sapstain fungus lasiodiplodia theobromae in rubberwood (hevea brasiliensis) were isolated, screened and identified by various morphological, biochemical and molecular techniques. the inhibitory effect of seventeen bacterial isolates was examined and seven exhibited inhibition towards the sapstain fungus. among the seven antagonists, six were conclusively identified as bacillus subtilis and one as paenibacill ... | 2014 | 25049165 |
| free-radical scavenging properties and antioxidant activities of botryosphaeran and some other β-d-glucans. | β-d-glucans are known to present antitumor, anticancer, and anti-inflammatory activities that are influenced by their own antioxidant capacity. the antioxidant activity of botryosphaeran, an exopolysaccharide of the (1 → 3;1 → 6)-β-d-glucan type produced by the botryosphaeria rhodina mamb-05 was evaluated and compared to some other β-d-glucans (lasiodiplodan an exocellular (1 → 6)-β-d-glucan from lasiodiplodia theobromae, laminarin and curdlan), and oligosaccharides, disaccharides, and monosacch ... | 2015 | 25128096 |
| theobroxide treatment inhibits wild fire disease occurrence in nicotiana benthamiana by the overexpression of defense-related genes. | theobroxide, a novel compound isolated from a fungus lasiodiplodia theobromae, stimulates potato tuber formation and induces flowering of morning glory by initiating the jasmonic acid synthesis pathway. to elucidate the effect of theobroxide on pathogen resistance in plants, nicotiana benthamiana plants treated with theobroxide were immediately infiltrated with pseudomonas syringae pv. tabaci. exogenous application of theobroxide inhibited development of lesion symptoms, and growth of the bacter ... | 2013 | 25288936 |
| proteomic analysis of responsive stem proteins of resistant and susceptible cashew plants after lasiodiplodia theobromae infection. | gummosis is an aggressive disease caused by the necrotrophic fungus lasiodiplodia theobromae (pat.) griffon & maubl that threatens commercial cashew orchads in brazil. to study the molecular mechanisms involved in the cashew response to l. theobromae, a proteomic analysis of stems from the commercial cashew clone brs 226 (resistant) was conducted at early times post-artificial infection. in addition, changes in the stem proteome profiles of gummosis resistant and susceptible cashew plants grown ... | 2015 | 25289588 |
| purification, characterization and application of lipoxygenase isoenzymes from lasiodiplodia theobromae. | lipoxygenase oxidizes linoleic acid into hydroperoxy octadecadienoic acid (hpod), which is important in food and flavour industries for production of bread and flavouring compounds. as lasiodiplodia theobromae is an unexplored, good source of lipoxygenase, it was purified from it by size-exclusion (sephadex g100) and ion-exchange (deae-cellulose) chromatography and characterized. upon purification, l. theobromae was found to contain two different lipoxygenases, one of 93 kda (lox1) and another o ... | 2015 | 25326184 |
| high incidence of preharvest colonization of huanglongbing-symptomatic citrus sinensis fruit by lasiodiplodia theobromae (diplodia natalensis) and exacerbation of postharvest fruit decay by that fungus. | huanglongbing (hlb), presumably caused by the bacterium "candidatus liberibacter asiaticus," is a devastating citrus disease associated with excessive preharvest fruit drop. lasiodiplodia theobromae (diplodia) is the causal organism of citrus stem end rot (ser). the pathogen infects citrus fruit under the calyx abscission zone (az-c) and is associated with cell wall hydrolytic enzymes similar to plant enzymes involved in abscission. by means of dna sequencing, diplodia was found in "ca. liberiba ... | 2015 | 25344245 |
| identification and evaluation of strain b37 of bacillus subtilis antagonistic to sapstain fungi on poplar wood. | devaluation of poplar products by sapstain accounts for huge and unpredictable losses each year in china. we had isolated four poplar sapstain fungi, ceratocystis adiposa hz91, lasiodiplodia theobromae ym0737, l. theobromae fx46, and fusarium sp. ym05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties. after being experimented with agar plates, wood chips, and enzyme activities, strain b37 was identified as the best poplar sapstain biocontrol bacterium. the stra ... | 2014 | 25401124 |
| β-(1 → 3)-glucanolytic yeasts from brazilian grape microbiota: production and characterization of β-glucanolytic enzymes by aureobasidium pullulans 1wa1 cultivated on fungal mycelium. | a total of 95 yeast strains were isolated from the microbiota of different grapes collected at vineyards in southern brazil. the yeasts were screened for β-(1 → 3)-glucanases using a newly developed zymogram method that relies upon the appearance of clearance zones around growing colonies cultured on agar–botryosphaeran medium and also by submerged fermentation on nutrient medium containing botryosphaeran, a (1 → 3),(1 → 6)-β-d-glucan. among 14 β-(1 → 3)-glucanase-positive yeasts identified, fou ... | 2015 | 25559084 |
| efficacy of oryza sativa husk and quercus phillyraeoides extracts for the in vitro and in vivo control of fungal rot disease of white yam (dioscorea rotundata poir). | tuber rot disease is a major constraint to white yam (dioscorea rotundata) production, accounting for 50-60% of annual yield losses in nigeria. the main method of control using synthetic fungicides is being discouraged due to human and environmental health hazards. the potential of oryza sativa husk (osh) and quercus phillyraeoides (qp) extracts for the in vitro and in vivo control of six virulent rot-causing fungal pathogens, lasiodiplodia theobromae, aspergillus niger, rhizoctonia solani, peni ... | 2014 | 25674452 |
| treatment with intrastromal and intracameral voriconazole in 2 eyes with lasiodiplodia theobromae keratitis: case reports. | to report the clinical presentation and the role of intrastromal and intracameral voriconazole injection in the management of rare cases of fungal keratitis caused by lasiodiplodia theobromae.two eyes of 2 patients with lasiodiplodia keratitis unresponsive to topical and oral antifungal medications were included in this study. diagnosis of lasiodiplodia keratitis was confirmed by microbiological analysis, including culture-based (case 1 and 2) and dna sequencing techniques (case 2 only).the firs ... | 2015 | 25674759 |
| [keratitis by lasiodiplodia theobromae: a case report and literature review]. | we report a case of mycotic keratitis caused by lasiodiplodia theobromae in a 60-year-old man with a history of ocular trauma with vegetable matter. ophthalmological assessment with slit-lamp and microbiological evaluation of the corneal ulcer by conventional microbiological techniques were performed. mycology study of the corneal scraping showed the presence of fungal filaments and the isolate was identified as lasiodiplodia theobromae. patient was treated with natamycin 5% and fluconazole 0.2% ... | 2014 | 25679935 |
| characterisation of the metabolites of an antibacterial endophyte botryodiplodia theobromae pat. of dracaena draco l. by lc-ms/ms. | botryodiplodia theobromae pat. belongs to the endophytic fungi that live within the tissues of medicinal plants and produce bioactive natural products. the endophyte was isolated from the leaves of dracaena draco l. the lc-ms-based metabolite fingerprinting of the ethyl acetate extract of b. theobromae with antibacterial activity led to the identification of 13 metabolites pertaining to various classes: dipeptides (maculosin and l,l-cyclo(leucylprolyl), alkaloid (norharman), coumarin and isocoum ... | 2015 | 25693860 |
| modulation of gene expression and cell cycle by botryosphaeran, a (1→3)(1→6)-β-d-glucan in human lymphocytes. | there is growing interest in the anticancer and immunomodulatory potential of fungal β-d-glucans. in the present study, the modulation of gene expression via rt-qpcr and cell cycle kinetics via flow cytometry were assessed in human normal and tumor (jurkat) lymphocytes after treatment with botryosphaeran (a fungal (1→3)(1→6)-β-d-glucan) from botryosphaeria rhodina mamb-05. cell cultures were treated with botryosphaeran either alone, or in combination with doxorubicin (dxr), in a post-treatment p ... | 2015 | 25795388 |
| an unusual skin lesion caused by lasiodiplodia theobromae. | we describe a case of subcutaneous infection as a result of traumatic implantation caused by the fungus lasiodiplodia theobromae. it was isolated in multiple swabs from the foot of an active healthy male. the fungus was identified by traditional mycology culture methods though this was slow with much time required for sporulation on only one of the agars used. identification was confirmed by dna sequencing. the patient was successfully treated with voriconizole. | 2015 | 25893164 |
| carboxymethylation of (1 → 6)-β-glucan (lasiodiplodan): preparation, characterization and antioxidant evaluation. | d-glucans possess immunomodulatory activities and potential for the development of new therapeutic agents. biological activities can be enhanced in these biopolymers through chemical derivatization, e.g., carboxymethylation. this work presents the carboxymethylation, characterization and the evaluation of antioxidant activities of the exocellular (1 → 6)-β-d-glucan produced by lasiodiplodia theobromae mmpi. thermal analysis indicated that the native and carboxymethylated polysaccharides presente ... | 2015 | 25965498 |
| chemical composition, antioxidant and antimicrobial activities of basil (ocimum basilicum) essential oils depends on seasonal variations. | chemical composition, antioxidant and antimicrobial activities of the essential oils from aerial parts of basil (ocimum basilicum l.) as affected by four seasonal, namely summer, autumn, winter and spring growing variation were investigated. the hydro-distilled essential oils content ranged from 0.5% to 0.8%, the maximum amounts were observed in winter while minimum in summer. the essential oils consisted of linalool as the most abundant component (56.7-60.6%), followed by epi-α-cadinol (8.6-11. ... | 2008 | 26065762 |
| caulicolous botryosphaeriales from thailand. | members of botryosphaeriales are commonly encountered as endophytes or pathogens of various plant hosts. the botryosphaeriaceae represents the predominant family within this order, containing numerous species associated with canker and dieback disease on a wide range of woody hosts. during the course of routine surveys from various plant hosts in thailand, numerous isolates of botryosphaeriaceae, including aplosporellaceae were collected. isolates were subsequently identified based on a combinat ... | 2015 | 26240447 |
| antiproliferative and pro-apoptotic effects of three fungal exocellular β-glucans in mcf-7 breast cancer cells is mediated by oxidative stress, amp-activated protein kinase (ampk) and the forkhead transcription factor, foxo3a. | fungal β-d-glucans of the (1→3)-type are known to exhibit direct antitumor effects, and can also indirectly decrease tumor proliferation through immunomodulatory responses. the underlying molecular mechanisms involved in decreasing tumor formation, however, are not well understood. in this study, we examined the antiproliferative role and mechanism of action of three different fungal exocellular β-glucans in mcf-7 breast cancer cells. the β-glucans were obtained from botryosphaeria rhodina mamb- ... | 2015 | 26255117 |
| antifungal and proteolytic activities of endophytic fungi isolated from piper hispidum sw. | endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. in the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and altern ... | 2015 | 26273250 |
| isolation, purification and characterization of a novel steroidal saponin cholestanol glucoside from lasiodiplodia theobromae that induces apoptosis in a549 cells. | search for novel anticancer lead molecules continues to be a major focus of cancer research due to the limitations of existing drugs such as lack of tumor selectivity, narrow therapeutic index and multidrug resistance of cancer types. natural molecules often possess better pharmacokinetic traits compared to synthetic molecules as they continually evolve by natural selection process to interact with biological macromolecules. microbial metabolites constitute nearly half of the pharmaceuticals in ... | 2016 | 26338072 |
| (1→6)- and (1→3)(1→6)-β-glucans from lasiodiplodia theobromae mmbj: structural characterization and pro-inflammatory activity. | the chemical composition and structural characterization of exopolysaccharides from the fungus lasiodiplodia theobromae mmbj are described, and the immunomodulatory activity of a purified β-glucan was evaluated. l. theobromae mmbj produced three different β-glucans. one, fraction peps, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. the other two, fractions seps-005r and seps-10e, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)da and 7.0×10(3)da, res ... | 2015 | 26344312 |
| low doses of gamma radiation in the management of postharvest lasiodiplodia theobromae in mangos. | the postharvest life of mango is limited by the development of pathogens, especially fungi that cause rot, among which stands out the lasiodiplodia theobromae. several control methods have been employed to minimize the damages caused by this fungus, chemical control can leave residues to man and nature; physical control by the use of gamma radiation in combination with modified atmosphere and cold storage. the use of gamma radiation helps to reduce the severity of the pathogen assist in the ripe ... | 2015 | 26413068 |
| fatty acid esters produced by lasiodiplodia theobromae function as growth regulators in tobacco seedlings. | the botryosphaeriaceae are a family of trunk disease fungi that cause dieback and death of various plant hosts. this work sought to characterize fatty acid derivatives in a highly virulent member of this family, lasiodiplodia theobromae. nuclear magnetic resonance and gas chromatography-mass spectrometry of an isolated compound revealed (z, z)-9,12-ethyl octadecadienoate, (trivial name ethyl linoleate), as one of the most abundant fatty acid esters produced by l. theobromae. a variety of natural ... | 2016 | 26926564 |
| identification and characterization of lipopeptides from bacillus subtilis b1 against sapstain fungus of rubberwood through maldi-tof-ms and rt-pcr. | bacillus subtilis is a potent biocontrol agent producing a wide array of antifungal lipopeptides for the inhibition of fungal growth. b. subtilis b1 isolated from market-available compost provided an efficient control of rubberwood sapstain fungus, lasiodiplodia theobromae. the current study is aimed to identify and characterize the lipopeptides responsible for the biocontrol of rubberwood sapstain fungus by bacillus subtilis b1. the bacterial whole-cell surface extract from the dual culture of ... | 2016 | 27004481 |
| microbial diversity in the gut of cashew stem girdler, analeptes trifasciata fabricius (coleoptera: cerambycidae), in ibadan, nigeria. | the cashew stem girdler, analeptes trifasciata, is a major insect pest of cashew in nigeria causing economic damage in cashew plantations even at low density. in this study, newly emerged adults of a. trifasciata reared from field-infested cashew stems were collected from the rearing cages, sexed, and dissected to reveal the internal structures of the insects. the gut was excised and separated into the foregut, midgut, and hindgut. the dissected gut compartments were blotted dry by sandwiching i ... | 2016 | 27147898 |
| protective effects of the exopolysaccharide lasiodiplodan against dna damage and inflammation induced by doxorubicin in rats: cytogenetic and gene expression assays. | the lasiodiplodan (ls) is a β-(1→6)-d-glucan produced by the fungus lasiodiplodia theobromae and some of the biological activities of ls were reported as hypoglycemic, anticoagulant, anti-proliferative and anticancer action; however, its effects on dna instability and modulation of gene expression are still unclear. aims of study were investigate the genotoxic effects of lasiodiplodan, and its protective activity against dna damage induced by doxorubicin (dxr) and its impact on the expression of ... | 2017 | 27181935 |
| comparative assessment of fungal augmentation treatments of a fine-textured and historically oil-contaminated soil. | the removal of aged hydrophobic contaminants from fine-textured soils is a challenging issue in remediation. the objective of this study was to compare the efficacy of augmentation treatments to that of biostimulation in terms of total aliphatic hydrocarbon (tah) and toxicity removal from a historically contaminated clay soil and to assess their impact on the resident microbial community. to this aim, pleurotus ostreatus, botryosphaeria rhodina and a combination of both were used as the inoculan ... | 2016 | 27220102 |
| quantitative real-time pcr normalization for gene expression studies in the plant pathogenic fungi lasiodiplodia theobromae. | lasiodiplodia theobromae is a highly virulent plant pathogen. it has been suggested that heat stress increases its virulence. the aim of this work was to evaluate, compare, and recommend normalization strategies for gene expression analysis of the fungus growing with grapevine wood under heat stress. using rt-qpcr-derived data, reference gene stability was evaluated through genorm, normfinder and bestkeeper applications. based on the geometric mean using the ranking position obtained for each in ... | 2016 | 27237774 |