Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| differential responses of bovine macrophages to mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium. | mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium are antigenically and genetically similar organisms; however, they differ in their virulence for cattle. m. avium subsp. paratuberculosis causes a chronic intestinal infection leading to a chronic wasting disease termed paratuberculosis or johne's disease, whereas m. avium subsp. avium causes only a transient infection. we compared the response of bovine monocyte-derived macrophages to ingestion of m. avium subsp. p ... | 2002 | 12228282 |
| histologic and genotypic characterization of a novel mycobacterium species found in three cats. | three cases of feline atypical mycobacteriosis from different geographical regions in north america were characterized by large clusters of filamentous bacteria visible on hematoxylin-and-eosin-stained tissue sections. pcr amplification demonstrated the presence of mycobacterium-specific nucleic acid in samples of skin lesions from these cases. pcr-assisted cloning and dna sequence analysis of a 541-bp length of the mycobacterium 16s rrna gene generated dna sequences which were >95% identical, s ... | 2002 | 12089257 |
| mycobacterium avium and mycobacterium intracellulare infection in mammals. | mycobacterium avium subsp. avium and m. intracellulare are ubiquitous organisms in the environment. the reservoir of m. avium subsp. avium is generally accepted to be environmental, in particular, water and soil are sources of the organism. in contrast to m. avium infection in wild and domestic birds, m. avium infection in mammals occurs only sporadically and is rarely transmissible. generalised disease is usually uncommon, owing to the non-progressive, chronic character of the infection. howeve ... | 2001 | 11288512 |
| identification and sub-typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium by randomly amplified polymorphic dna. | a commercially available kit consisting of twenty 10-mer random primers was evaluated to allow selection of a suitable primer that would permit identification and sub-typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium by randomly amplified polymorphic dna (rapd). a primer ope-20 (5'-aac-ggt-gac-c-3') was identified to be the most suitable primer when tested with four atcc reference strains of m. paratuberculosis and eight well characterized field strains e ... | 2001 | 11240105 |
| regulation of expression of major histocompatibility antigens by bovine macrophages infected with mycobacterium avium subsp. paratuberculosis or mycobacterium avium subsp. avium. | mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium are antigenically and genetically very similar organisms; however, they differ markedly in their virulence for cattle. we evaluated the capacity of bovine macrophages infected with m. avium subsp. paratuberculosis or m. avium subsp. avium to express major histocompatibility complex (mhc) class i and class ii antigens on their surface and to interact with primed autologous lymphocytes. our results indicate that infec ... | 2001 | 11159996 |
| comparison of two enzyme-linked immunosorbent assays for serologic diagnosis of paratuberculosis (johne's disease) in cattle using different subspecies strains of mycobacterium avium. | serologic diagnosis of bovine paratuberculosis (johne's disease) with currently available tests may give false-positive results due to cross-reactions with avian and bovine tuberculosis viruses and other infectious agents. indirect enzyme-linked immunosorbent assays (elisa) for detection of antibodies against paratuberculosis based on antigens from mycobacterium avium subsp. avium (a-elisa) and m. avium subsp. paratuberculosis (p-elisa) were compared. despite an expected higher specificity for m ... | 2001 | 11289215 |
| alkyl hydroperoxide reductases c and d are major antigens constitutively expressed by mycobacterium avium subsp. paratuberculosis. | antigens characteristic for mycobacterium avium subspecies paratuberculosis were identified by crossed immunoelectrophoresis (cie) and by absorbing out cross-reactive antigens by using a polyclonal and polyvalent mycobacterium avium subspecies avium antiserum. two antigens were present in m. avium subsp. paratuberculosis and not detected in mycobacterium avium subsp. avium. they were identified as antigens 17 and 20 in a cie reference system for m. avium subsp. paratuberculosis antigens. purifie ... | 2000 | 10639449 |
| a pre-existing infection by mycobacterium avium subsp. avium modulates anti-cryptococcus neoformans and anti-candida albicans activities in human macrophages. | mycobacterium avium is a facultative intracellular microorganism, able to survive and multiply within mammalian macrophages by circumventing antimicrobial mechanisms. in this study we hypothesize that pre-existing m. avium infection could result in macrophage superinfections by other microorganisms. we found that 24 h after ingestion of m. avium at a low multiplicity of infection, macrophages are unable to efficiently produce superoxide anions when over-stimulated with phorbol esters, and that t ... | 2000 | 10906264 |
| further studies on the gs element. a novel mycobacterial insertion sequence (is1612), inserted into an acetylase gene (mpa) in mycobacterium avium subsp. silvaticum but not in mycobacterium avium subsp. paratuberculosis. | we have recently described the gs element, found in mycobacterium avium subsp. paratuberculosis (map), mycobacterium avium subsp. silvaticum (mas) and some isolates of mycobacterium avium subsp. avium serotype 2 (maas2), which contains a set of genes of low gc% content, putatively associated with the biosynthesis, modification and transference of fucose to cell wall glycopeptidolipids. here we describe a further gene of low gc% content (mpa), within the gs element in map. mpa is a putative acety ... | 2000 | 11118730 |
| distinct differences in repertoires of low-molecular-mass secreted antigens of mycobacterium avium complex and mycobacterium tuberculosis. | antigens in a 4-week-old culture filtrate (cf) of mycobacterium avium subsp. avium were separated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified by western blotting. the culture had minimal lysis of bacilli, giving a cf preparation consisting mainly of secreted proteins. comparison with a similar cf of mycobacterium tuberculosis with almost no contamination with intracellular proteins showed the presence of cross-reactive antigens homologous to the four components of ... | 2000 | 11101579 |
| comparison of is1245 restriction fragment length polymorphism and pulsed-field gel electrophoresis for typing clinical isolates of mycobacterium avium subsp avium. | little is still known about the epidemiology and pathogenesis of mycobacterium avium subsp avium (masa) infection. | 2000 | 10815741 |
| detection of mycobacterium avium subspecies avium in formalin-fixed, paraffin-embedded tissues of captive exotic birds using polymerase chain reaction. | a presumptive diagnosis of avian tuberculosis can be made when characteristic histologic lesions and acid-fast bacilli are observed in avian tissue samples. however, a definitive diagnosis requires isolation and identification of the causative organism, a process that can take several weeks to complete. the purpose of the study was to determine whether formalin-fixed, paraffin-embedded archival avian tissues could be tested by polymerase chain reaction (pcr) to reliably and rapidly diagnose avia ... | 1999 | 10572856 |
| occurrence of the is900 gene in mycobacterium avium complex derived from hiv patients. | the occurrence of the insertion sequence is900 in mycobacterium avium subsp. avium strains isolated from hiv infected patients has been investigated. in this study, genomic dna from 62 mycobacterial isolates [31 strains of m. avium complex (mac) consisting of 26 m. avium subsp. avium hiv-isolates and five non-hiv isolates and 31 additional mycobacterium species] were analysed by an is900 -based polymerase chain reaction (pcr) assay and southern hybridization using a non-radioactive-labelled 251 ... | 1999 | 10508558 |
| pcr-restriction endonuclease analysis for identification and strain typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium based on polymorphisms in is1311. | point mutations in the is 1311 sequences from sheep and cattle strains of mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) and m. avium subsp. avium (m. avium) were targeted to develop a polymerase chain reaction (pcr) that would be useful in the diagnosis and control of johne's disease. candidate pcr tests were evaluated for sensitivity, specificity and ease of interpretation of the restriction endonuclease analysis (rea) products. one is 1311 pcr, amplifying a 608 base pair pr ... | 1999 | 10208802 |
| typing of clinical mycobacterium avium complex strains cultured during a 2-year period in denmark by using is1245. | in the present study restriction fragment length polymorphism analyses with the recently described insertion sequence is1245 as a probe was performed with clinical mycobacterium avium complex strains cultured in denmark during a 2-year period. the overall aim of the study was to disclose potential routes of transmission of these microorganisms. as a first step, the genetic diversity among isolates from aids patients and non-human immunodeficiency virus (hiv)-infected patients was described. in a ... | 1999 | 9986819 |
| rapid differentiation of mycobacterium avium and m. paratuberculosis by pcr and restriction enzyme analysis. | mycobacterium avium subsp. avium (m. avium) and m. avium subsp. paratuberculosis (m. paratuberculosis), intracellular bacteria that can cause chronic granulomatous enteritis in cattle, are difficult to distinguish on the basis of growth and biochemical characteristics. we report the development of a pcr-based strategy for the rapid differentiation of isolates of m. avium from isolates of m. paratuberculosis. restriction fragment length polymorphism was identified by pcr amplification and subsequ ... | 1996 | 8904449 |
| numerical taxonomy of mycobactin-dependent mycobacteria, emended description of mycobacterium avium, and description of mycobacterium avium subsp. avium subsp. nov., mycobacterium avium subsp. paratuberculosis subsp. nov., and mycobacterium avium subsp. silvaticum subsp. nov. | we performed a numerical taxonomy analysis of 38 mycobacterium paratuberculosis and related mycobacterial strains, including wood pigeon mycobacteria; this analysis was based on 22 tests, which were selected for their potential discriminative value from a total of 51 tests studied and produced four well-defined clusters. cluster 1 contained the m. paratuberculosis strains, including two strains isolated from crohn's disease patients; cluster 2 contained mycobacterium avium and mycobacterium intr ... | 1990 | 2397193 |