| haemolytic disease associated with leptospira interrogans serovar pomona in red deer calves (cervus elaphus). | three red deer calves (cervus elaphus) died with a haemolytic disease associated with infection by leptospira interrogans serovar pomona. infection within the herd was more prevalent than disease. sera from 16 herd mates were tested by the microscopic agglutination test (mat) and 12 had leptospiral titres, the majority to serovar pomona. a few calves had titres to balcunica and hardjo. urine was obtained for culture from six of these calves and serovar pomona was isolated from five with titres t ... | 1986 | 16031298 |
| variable nucleotide tandem-repeat analysis revealing a unique group of leptospira interrogans serovar pomona isolates associated with california sea lions. | leptospira interrogans serovar pomona isolates were compared by variable nucleotide tandem-repeat typing. most cattle isolates grouped together, while isolates from pigs and wildlife were distributed across several groups. significantly, california sea lion isolates formed a unique group, providing evidence that these animals are maintenance hosts of serovar pomona. | 2009 | 19204099 |
| are white-spot lesions in kidneys in sheep associated with leptospirosis? | to determine the association between white-spot lesions in kidneys and serological and cultural prevalence of leptospirosis in sheep, and to evaluate the diagnostic value of these lesions in individual sheep and lines of sheep at slaughter as indicators of past or current episodes of leptospirosis. | 2009 | 19252540 |
| differential response of cytokines induced by leptospira interrogans, serogroup pomona, serovar pomona, in mouse and human cell lines. | leptospira interrogans, the causative agent of leptospirosis, is an important zoonotic bacterium. the mechanisms and roles of cytokine induction in both humans and animals remain unclear. therefore, the ifn-gamma, il-6, il-10 and il-12 levels were measured by enzyme-linked immunosorbent assay (elisa) in human thp-1 and mouse raw 264.7 monocyte cell lines following stimulation with heat-killed leptospira interrogans serogroup pomona serovar pomona, l. biflexa, e. coli or salmonella group b. the p ... | 2008 | 19317342 |
| [development of a method to determine the minimum inhibitory concentration in reference strains from leptospiras]. | a method to determine the minimum inhibitory concentration for leptospiras was developed, since there is not a standard method to measure it at the international level. reference strains from the pathogenic complex l. interrogans and l. biflexa were used against penicillin, cyprofloxacine, chloramphenicol, rifampicine and tetracycline. the minimum inhibitory concentration was defined as the lowest concentration of antibiotic where it was observed the inhibition of the bacterial mobility by direc ... | 2005 | 17966469 |
| a serological survey of leptospirosis in cattle of rural communities in the province of kwazulu-natal, south africa. | a serological survey of leptospirosis in cattle originating from rural communities of the province of kwazulu-natal (kzn) in south africa was carried out between march 2001 and december 2003. the survey was designed as a 2-stage survey, using the local diptank as the primary sampling point. in total, 2021 animals from 379 diptanks in 33 magisterial districts were sampled and tested with the microscopic agglutination test (mat). the apparent prevalence at district level was adjusted for clusterin ... | 2009 | 19653519 |
| immune response and prophylactic efficacy of smegmosomes in a hamster model of leptospirosis. | leptospirosis is an important zoonotic disease worldwide. subunit vaccines are an attractive intervention strategy against this disease, but potent, non-toxic adjuvants are necessary components to any effective vaccine. among various adjuvant candidates, liposomes have garnered recent attention for their capacity as carriers of vaccines. in the present study we prepared novel liposomes using total polar lipids from the nonpathogenic bacterium, mycobacterium smegmatis (designated smegmosomes). th ... | 2009 | 19715780 |
| leptosome-entrapped leptospiral antigens conferred significant higher levels of protection than those entrapped with pc-liposomes in a hamster model. | we prepared novel liposomes from total polar lipids of non-pathogenic leptospira biflexa serovar potac (designated leptosomes) and evaluated their vaccine delivery/adjuvant potential with novel protective antigens (lp0607, lp1118 and lp1454) of l. interrogans serovar pomona in a hamster model. the immune response induced by three individual antigens and protective efficacy were evaluated and compared to those induced by same antigens entrapped with pc-liposomes and e. coli lipid liposomes (esche ... | 2009 | 19729088 |
| identification and characterization of ompa-like proteins as novel vaccine candidates for leptospirosis. | leptospira is an important infectious gram-negative bacterium causing leptospirosis in mammals. outer membrane proteins (omps) are key molecules in the interface between the cell and its environment. a group of putative leptospiral outer membrane proteins with an ompa-like domain, comprising lp0056, lp0222, lp3615, lp3685, lp4337 and lbp328, were identified by bioinformatic methods and expressed as gst-tag fusion proteins. all these recombinant proteins were screened for immune-protective potent ... | 2010 | 20064477 |
| experimental leptospirosis in capybaras (hydrochaeris hydrochaeris) infected with leptospira interrogans serovar pomona. | capybara (hydrochaeris hydrochaeris), the largest rodent in the world, is widely distributed in south america. these animals live in areas with abundant water, which makes them a potential reservoir for leptospira. the objective of this study was to investigate seroconversion, leptospiremia, and leptospiruria in capybaras experimentally infected with a virulent strain of leptospira interrogans serovar pomona. seven capybaras were used: one control and six infected. agglutinins against serovar po ... | 2009 | 20063819 |
| a unique genotype of leptospira interrogans serovar pomona type kennewicki is associated with equine abortion. | although serologic data indicate horses in n. america are exposed to a variety of leptospiral serovars, abortion is almost always associated with leptospira interrogans serovar pomona type kennewicki. a variety of wildlife including raccoons, white tailed deer, striped skunks, opossums, and red and grey foxes have been shown to host serovar pomona and have therefore been suspect as sources of infection for pregnant mares. the aim of the present study was to examine genetic diversity in serovar p ... | 2011 | 21450416 |
| cytopathic effect in bhk 21 (c13) cells inoculated with leptospira interrogans serovar pomona isolated from a porcine abortion. | | 2009 | 20085192 |
| serologic evaluation of new zealand sea lions for exposure to brucella and leptospira spp. | a serologic survey of anti-brucella and antileptospiral antibodies was conducted on 147 adult, female new zealand sea lions (phocarctos hookeri). most sea lions (n=138) were sampled at sandy bay, enderby island, auckland islands (50°30's, 166°17'e), january 2000-march 2005. nine were sampled at otago, new zealand (46°0's, 170°40'e); four in april 2008 and five in march 2009. serum from one of the enderby island females was weakly positive for antibodies to brucella abortus using the competitive ... | 2010 | 20966283 |
| investigation of localisation of leptospira spp. in uterine and fetal tissues of non-pregnant and pregnant farmed deer. | to find evidence for localisation in the uterus, and fetal infection, of leptospira spp. in farmed deer in the lower north island of new zealand during and shortly after the breeding season. | 2010 | 21151213 |
| [ultramicroscopic alterations in placental tissue from infected animals with leptospira interrogans serovar pomona]. | | 2011 | 21491071 |
| cyclical changes in seroprevalence of leptospirosis in california sea lions: endemic and epidemic disease in one host species? | leptospirosis is a zoonotic disease infecting a broad range of mammalian hosts, and is re-emerging globally. california sea lions (zalophus californianus) have experienced recurrent outbreaks of leptospirosis since 1970, but it is unknown whether the pathogen persists in the sea lion population or is introduced repeatedly from external reservoirs. | 2007 | 17986335 |
| [leptospirosis outbreak in calves from corrientes province, argentina]. | leptospirosis is an infectious disease resulting in significant economic losses in livestock production. this disease causes abortion, embryo death, death of calves within the first few days of life and mastitis. we report a leptospirosis outbreak in calf growing and fattening. histopathological and hemoparasite studies, immunofluorescence, and bacterial cultures were performed. a strain of leptospira interrogans serovar pomona was isolated from samples collected from dead calves. | 2011 | 21491066 |
| [identification of leptospira interrogans serovar pomona in mice genetically selected for high and low antibody production, by means of the immunoperoxidase technique in renal tissue and bacterial isolation in fletcher medium]. | the present work had the objective of identifying the presence of leptospira interrogans serovar pomona in mice that had been genetically selected for high and low response to antibodies. all the animals were subjected to bacterial isolation, immunohistochemical analysis (immunoperoxidase) in renal tissue sections and hematoxylin-eosin staining. the immunoperoxidase technique was little more sensitive than culturing, but both were good parameters for agent identification. more severe renal lesio ... | 2007 | 18200418 |
| leptospira immunoglobulin-like protein a variable region (ligavar) incorporated in liposomes and plga microspheres produces a robust immune response correlating to protective immunity. | subunit vaccines are attractive as an intervention strategy against leptospirosis, an important zoonotic disease afflicting both humans and livestock. however, the success of subunit vaccines has been hampered by weak or short-term immunity and unavailability of nontoxic, potent adjuvants. in the present study, the variable region of recombinant leptospira immunoglobulin like protein a (ligavar) incorporated into conventional liposomes and plga microspheres produced robust immune responses that ... | 2009 | 19022317 |
| immunogenicity and protective efficacy of recombinant leptospira immunoglobulin-like protein b (rligb) in a hamster challenge model. | leptospiral immunoglobulin-like protein (ligb) was truncated into conserved (ligbcon) and variable (varb1, varb2) fragments and expressed as gst/his-tag fusion proteins. four-week-old hamsters were immunized with equal amounts of each fragment individually or combined in alum adjuvant at days 0 and 21 and subsequently challenged three weeks after the booster with 2.5 ld(50) live virulent leptospira interrogans serovar pomona. our results demonstrate that immunization with ligb produced strong hu ... | 2009 | 19070678 |
| expression in escherichia coli of flab, the gene coding for a periplasmic flagellin of leptospira interrogans serovar pomona. | a periplasmic flagellin gene, flab, of leptospira interrogans serovar pomona (strain pomona) was expressed in escherichia coli for the production and antigenic characterisation of the protein. the flab structural gene, which was previously cloned into puc118, was derived by pcr from the recombinant plasmid and used to generate an expression construct with the trc promoter-driven pproex ht system. under the conditions employed, the flab was expressed as inclusion bodies formed within e. coli, yie ... | 1999 | 10535640 |
| growth response and shedding of leptospira spp. in urine following vaccination for leptospirosis in young farmed deer. | abstract | 2012 | 22175424 |
| proteome analysis of leptospira interrogans virulent strain. | leptospirosis is a worldwide zoonotic infection of human and veterinary concern. caused by pathogenic spirochetes of the genus leptospira, the disease presents greater incidence in tropical and subtropical regions. the identification of proteins that could be involved in the bacteria host interactions may facilitate the search for immune protective antigens. we report the proteomic analysis of leptospira interrogans serovar pomona virulent strain lpf cultured from kidney and liver of infected ha ... | 2009 | 19590580 |
| prevalence of pathogenic leptospira spp. in sheep in a sheep-only abattoir in new zealand. | to determine the prevalence of the two most commonly diagnosed pathogenic leptospira spp. serovars, hardjobovis and pomona, in sheep in a sheep-only abattoir in new zealand, and to determine the prevalence of kidneys which were leptospire culture-positive collected from sheep seropositive or seronegative to the microscopic agglutination test (mat). | 2008 | 18690252 |
| serosurvey of leptospirosis and investigation of a possible novel serovar arborea in farmed deer in new zealand. | to investigate the prevalence of leptospira spp. and possible novel serovar arborea infection in farmed deer in new zealand. | 2011 | 21541888 |