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a survey on chemical constituents and indications of aromatic waters soft drinks (hydrosols) used in persian nutrition culture and folk medicine for neurological disorders and mental health.in persian nutrition culture, drinking aromatic waters (hydrosols, distillate) has a long history as functional beverages or therapeutic remedies. the co-distilled water with essential oils, which contains partial amounts of more water-soluble volatile compounds are diluted and used as beverages. since the solubility of volatile components is different in water, the overall composition, and thus the biological activities of aromatic waters seem to be different from the essential oils they were c ...028633539
[study of citrus aurantium, linné, subsp. sinensis, gallesio, and its essence]. 194720262012
[study of citrus aurantium, linné subsp. sinensis, gallesio, and its essence]. 194720262010
[cysteine desulfhydrase in citrus aurantium]. 195313126311
[methoxylated flavones of citrus aurantium l. subspecies amara l]. 19685250536
citrus tree decline caused by pratylenchus coffeae.the pathogenic effects of pratylenchus coffeae on growth and yield of tangelo (citrus paradisi x c. reticulata) scions grafted on rough lemon (c jambhiri), sour orange (c. aurantium) and 'cleopatra' mandarin (c. reticulata) rootstocks were evaluated under field conditions for 4 years. pratylenchus coffeae on inoculated trees increased to significantly damaging population densities on rough lemon rootstock the second year, on sour orange the third and on cleopatra mandarin the fourth year after p ...197319319354
water permeability of isolated cuticular membranes: the effect of cuticular waxes on diffusion of water.the water permeability of astomatous cuticular membranes isolated from citrus aurantium l. leaves, pear (pyrus communis l.) leaves and onion (allium cepa l.) bulb scales was determined before and after extraction of cuticular waxes with lipid solvents. in pear, the permeability coefficients for diffusion of tritiated water across cuticular membranes (cm) prior to extraction [p d(cm)] decreased by a factor of four during leaf expansion. in all three species investigated p d(cm) values of cuticula ...197624424766
water permeability of isolated cuticular membranes: the effect of ph and cations on diffusion, hydrodynamic permeability and size of polar pores in the cutin matrix.the upper astomatous cuticle of citrus aurantium l. leaves was isolated enzymatically or chemically, extracted with lipid solvents and used for the determination of water diffusion (p d ) and osmotic water permeability (p f ). the water permeability was strongly dependent on the ph value and the cations of the buffer solutions. in presence of monovalent alkali metal ions p d increased almost five fold between ph 3 and 11. the shape of the plot p d vs. ph suggests the presence of 3 different diss ...197624430686
control of citrus nematode tylenchulus semipenetrans on fine-textured soil with dbcp and oxamyl.three grapefruit orchards on sour orange rootstock were treated by metering dbcp (l,2-dibromo-3-chloropropane) at 56 kg(a.i.)/ha into 15 cm of water in a flood irrigation system. in orchards with 43-49% clay in the surface foot, dbcp reduced numbers of tylenchulus semipenetrans below control levels for 1.5-2.0 years. in the 3 orchards, dbcp treatment resulted in increases in yield or fruit size in the 2 seasons following treatment. no increases in yield, fruit size, or fruit numbers were observe ...197719305570
plant cuticles are polyelectrolytes with isoelectric points around three.the isoelectric points of isolated cuticles from citrus aurantium l. (3.15), prunus armeniaca l. (3.45), and pyrus communis l. (2.90) leaves were determined from membrane potentials. at ph values below the isoelectric point, cuticular membranes carry a net positive charge and are permselective to anions (determined using (82)br(-)). above the isoelectric point, they carry a net negative charge and are permselective to cations (determined using (24)na(+)). there are no gradients of fixed charges ...197716659804
effects of pratylenchus vulnus on the growth of sour orange.pratylenchus vulnus suppressed the growth of sour orange seedlings in greenhouse experiments. growth retardation (in height, in trunk diameter, and in dry top and root weights) was observed in inoculated plants growing in two soil types. population density, 13 months after inoculation, averaged more than 1,000 specimens/gm of fresh root. anatomical studies showed that p. vulnus prefers to attack the cortex and causes cavities among the cortical cells.197719305583
reaction of citrus rootstocks to meloidogyne javanica.the response of citrus spp. and related rootstocks to a population of meloidogyne javanica was evaluated in a screenhouse experiment. palestine and rangpur lime, rough lemon, sour orange, sexton and thentriton tangelo, and volkamer lemon were not infected by m. javanica. galls and tip swellings were observed on the roots of poncirus triloliata and troyer citrange. there was no evidence of nematode development. symptoms induced by the nematode were stelar division, syncytia formation in the vascu ...197819305835
water permeability of plant cuticles : dependence of permeability coefficients of cuticular transpiration on vapor pressure saturation deficit.using the system vapor/membrane/liquid, permeability coefficients of cuticular transpiration (p ct) were determined as functions of water activity in the vapor (a wv). enzymatically isolated cuticular membranes (cm) of citrus aurantium l. and nonisolated cm of onion bulb scales and eggplant fruits were investigated. p ct of citrus and eggplant cm decreased with decreasing a wv, while permeability coefficients of cm of onion were independent of a wv. extraction of soluble cuticular lipids (scl) f ...197924407329
composition of soluble cuticular lipids and water permeability of cuticular membranes from citrus leaves.water permeability and composition of soluble cuticular lipids of isolated cuticular membranes from leaves of citrus aurantium l. were investigated for 3 successive years. the average water permeability coefficient determined using 169 cuticular membranes was 1.09·10(-7) cm s(-1) with a standard deviation of 0.78·10(-7) cm s(-1). there were no significant differences in water permeability between years. cuticular membranes are characterized by a great variability in water permeability both withi ...197924318244
[use of an herbal combination with laxative action on duodenal peptic ulcer and gastroduodenitis patients with a concomitant obstipation syndrome].thirty two patients were treated--19 with duodenal ulcer and 19 with gastroduodenitis, all of them with concomitant obstipation syndrome. the patients were treated with a combination of symphitum officinalis and calendula officinales form the main disease. the obstipation syndrome was treated with a combination of rhamus frangula, citrus aurantium, carum carvi. the laxative herb combination was effective in 100 per cent of the patients--daily defecation in 90.6 per cent and every second day in 9 ...19817336705
[pesticide residues in the orange (citrus aurantium l. ssp. aurantium). i. determination of residues of organophosphate insecticides using gas chromatography]. 19817307085
[the effects of citrus aurantium and its active ingredient n-methyltyramine on the cardiovascular receptors (author's transl)]. 19817257808
[treatment of chronic colitis with an herbal combination of taraxacum officinale, hipericum perforatum, melissa officinaliss, calendula officinalis and foeniculum vulgare].twenty four patients with chronic non-specific colitis were treated with a herb combination. as a result from the treatment, the spontaneous and palpable pains along the large intestine disappeared in 95.83 per cent of the patients by the 15th day of their admission to the clinic. defecation became daily in the patients with obstipation syndrome, but a combination of rhamus frangula, citrus aurantium, c. carvi was added to the herb combination already indicated. defecation was normalized in pati ...19817336706
estimate of yield loss from the citrus nematode in texas grapefruit.chemical control of the citrus nematode, tylenchulus semipenetrans cobb, has consistently increased yield of grapefruit on sour orange rootstock in texas. in this study, data from chemical control tests conducted from 1973 to 1980 were analyzed to determine the relationship between nematode counts and grapefruit yield and fruit size. the correlation between yield and nematode counts was negative (r = -0.47) and highly significant (p < 0.01). the data best fit the exponential decay curve: y = 160 ...198219295756
gas permeability of plant cuticles : oxygen permeability.cuticles from the adaxial surface of citrus aurantium l. leaves and from the pericarp of lycopersicon esculentum l. and capsicum annuum l. were isolated enzymatically and their oxygen permeability was determined. isolated cuticles were mounted between a gaseous and an aqueous compartment with the physiological outer side of the membrane facing the gaseous compartment. permeability for oxygen was characterized by permeability (p) and diffusion (d) coefficients. p and d were independent of the dri ...198224271865
[pesticide residues in oranges (citrus aurantium l. ssp. aurantium). ii. organophosphate insecticide residues]. 19827083341
plant regeneration from citrus protoplasts: variability in methodological requirements among cultivars and species.nucellar callus lines were established from two orange cultivars ('nucellar shamouti', 'shamouti landau'), three mandarin cultivars ('murcott', 'dancy', 'ponkan') one grapefruit cultivar ('duncan') and sour orange (citrus aurantium). these callus lines were initiated from in vitro cultured ovules of young fruits and maintained an embryogenic capacity. the plating efficiencies of protoplasts derived from these calli, as well as those of protoplasts from lemon (cv. 'villafranca') nucellar callus w ...198224270567
relationships of leaf fatty acids to cold hardening of citrus seedlings.three cultivars of citrus with different sensitivities to freezing temperatures (citron, citrus medica l.; rough lemon, c. limon burm. f; sour orange, c. aurantium l.) were cold hardened for 4 weeks. lipids from leaves of hardened and control seedlings were fractionated and analyzed for fatty acids. the absolute amount of triglycerides and phospholipids increased in the leaves upon hardening. with hardening, total linoleic acid also increased 141% in citron, 210% in rough lemon, and 233% in sour ...198216662433
[biological characteristics and pruning technic of citrus aurantium l]. 19836235010
photosynthate partitioning in split-root citrus seedlings with mycorrhizal and nonmycorrhizal root systems.photosynthate partitioning was examined in seedings of sour orange (citrus aurantium l.) and carrizo citrange (poncirus trifoliata [l.] raf. x c. sinensis [l.] osbeck) grown with split root systems inoculated on one side with vesicular-arbuscular mycorrhizal fungus (glomus intraradices schenck and smith). source-sink relations were studied without mitigating differences in mineral content or physiological age that can occur in separate plant comparisons, because phosphorus was evenly distributed ...198416663589
accumulation and transport of (2,4-dichlorophenoxy)acetic acid in plant cuticles: i. sorption in the cuticular membrane and its components.partition coefficients (k) of (2,4-dichlorophenoxy)acetic acid (2,4-d) have been determined for the system plant cuticle/aqueous buffer. cuticles isolated enzymatically from leaves (clivia miniata, ficus elastica, citrus aurantium, hedera helix, pyrus communis cv. conférence and cv. williams, olea europaea) and fruits (lycopersicon esculentum, capsicum annuum, solanum melongena, cucumis sativus) were utilized. only the nondissociated species of 2,4-d was sorbed by cuticles and their lipophilic c ...19846734501
distribution of tylenchulus semipenetrans in a texas grapefruit orchard.distribution of the citrus nematode (tylenchulus semipenetrans) was studied over 18 months in a 6-year-old orchard of grapefruit (citrus paradisi cv. ruby red) on sour orange (c. aurantium) rootstock. the 1.8-ha orchard was under chemical weed control, no tillage, and flood irrigation. highest numbers of nematodes were found in the top 15 cm of the soil profile. the nematode population peaked in april and declined to lowest levels in august and september. numbers of nematodes were negatively cor ...198419294028
use of medicinal plants by ambulatory patients in puerto rico.the use of medicinal plants by the patients at the outpatient clinics of five health-care centers in puerto rico was evaluated. medication histories were obtained for 802 patients ranging in age from two months to 91 years. the most frequent medical diagnosis was cardiovascular disease (54% of the patients). respiratory and digestive disorders were the least frequent conditions, identified in only 9% and 6% of the cases, respectively. medicinal plants were used by 57% of the population. patients ...19846496496
[cultivation of citrus aurantium]. 19846235955
relation between ion accumulation of salt-sensitive and isolated stable salt-tolerant cell lines of citrus aurantium.four selected nacl-tolerant cell lines of sour orange (citrus aurantium) were compared with the nonselected cell line in their growth and internal ion content of na(+), k(+), and cl(-) when exposed to increasing nacl concentrations. no difference was found among the various nacl-tolerant cell lines in na(+) and cl(-) uptake, and all these cell lines took up similar or even larger amounts of na(+) and cl(-) than the nacl-sensitive cell line. exposure of cells of nacl-sensitive and nacl-tolerant l ...198516664188
allelopathic effects ofcitrus aurantium l. : ii. isolation, characterization, and biological activities of phytotoxins.in an earlier work, we found that the failure of herbaceous plants to grow under sour orange was mainly due to an allelopathic mechanism. four nonvolatile inhibitors were isolated by paper chromatography. color reactions of all the inhibitors indicated that they are phenolic in nature. on the other hand, five volatile inhibitors were detected by gas chromatography, four of which were terpenes. all inhibitors reduced seed germination and/or seedling growth ofamaranthus retroflexus.198524311245
allelopathic effects ofcitrus aurantium l. : i. vegetational patterning.field observations on undisturbed stands of sour orange revealed thatcynodon dactylon, chenopodium album, avena sativa, andamaranthus retroflexus were not able to grow normally and complete their life cycles under its canopies, although the same species grow well under adjacent trees of date palm. investigations revealed that the failure of the test species to grow normally under sour orange was not due to competition for light, moisture and minerals or to differences in soil texture or ph. soil ...198524311244
influence of the sting nematode, belonolaimus longicaudalus, on young citrus trees.the sting nematode, belonolaimus longicaudatus, was associated with poor growth of citrus in a central florida nursery. foliage of trees was sparse and chlorotic. affected rootstocks included changsha and cleopatra mandarin orange; flying dragon, rubidoux, and jacobsen trifoliate orange; macrophylla and milam lemon; palestine sweet lime; sour orange; and the hybrids - carrizo, morton, and rusk citrange and swingle citrumelo. root symptoms included apical swelling, development of swollen terminal ...198519294118
a study of the phototoxicity of lemon oil.lemon oil contains furocoumarin derivatives and is known to cause phototoxicity. in this study, lemon oil was fractionated, and its phototoxic activity was measured by means of a biological assay. the substances producing phototoxicity were identified by high-performance liquid chromatography as being oxypeucedanin and bergapten. the phototoxic potency of oxypeucedanin was only one-quarter of that of bergapten. however, the amounts of these two phototoxic compounds present in lemon oils produced ...19854096528
relaxant effects on tracheal and ileal smooth muscles of the guinea pig.the effects of volatile oils of 22 plants from 11 different families and of some of their constituents on tracheal and ileal smooth muscles were investigated. the results were compared with the relaxant effects of catecholamines and phosphodiesterase inhibitors. all of the oils had relaxant effects on the tracheal smooth muscle, the most potent were angelica root, clove, elecampane root, basil and balm leaves oil. 16 oils inhibited the phasic contractions of the ileal myenteric plexus-longitudin ...19854039178
flavanone glycosides as oviposition stimulants in a papilionid butterfly,papilio protenor.identification of chemical compounds responsible for the oviposition behavior in a rutaceae feeder,papilio protenor demetrius, was undertaken with the epicarp of sour orange (citrus natsudaidai) which exhibited potent stimulatory activity as did its leaves for egg-laying by the females. the stimulants were present in the hydrosoluble fraction, and the kairomonal activity displayed by the peel was regarded as originating from the synergistic effect of the total chemical complex. one of the active ...198624306464
the plastome of citrus. physical map, variation among citrus cultivars and species and comparison with related genera.a physical plastome map was constructed for citrus aurantium, and the plastomes of species and cultivars of citrus and of two citrus relatives were analysed by southern blot-hybridisation of labelled total tobacco cpdna to digests of total citrus dna. a resemblance was found between the plastomes of cultivars of c. limon (lemon), c. sinensis (orange), c. aurantium (sour orange), c. paradisii (grapefruit) and c. grandis (pomello). the plastomes of other citrus types such as mandarin (c. reticulat ...198624247831
relationship between salt tolerance and resistance to polyethylene glycol-induced water stress in cultured citrus cells.salt-tolerant selected cells of shamouti orange (citrus sinensis) and sour orange (citrus aurantium) grew considerably better than nonselected cells at any nacl concentration tested up to 200 millimolar. also, the growth response of each treatment was identical in the two species. however, the performance of cells of the two species under osmotic stress induced by polyethylene glycol (peg), which is presumably a nonabsorbed osmoticum, was significantly different. the nonselected shamouti cell li ...198716665715
air pollution effects on the structure of citrus aurantium leaves. 19873664002
evaluation of polyamine and proline levels during low temperature acclimation of citrus.the polyamines (pa) putrescine (put), spermidine (spd), and spermine (spm) were measured during 3 weeks exposure to cold hardening (15.6 degrees c day and 4.4 degrees c night) and nonhardening (32.2 degrees c day and 21.1 degrees c night) temperature regimes in three citrus cultivars: sour orange (so) (citrus aurantium l.), ;valencia' (val) (citrus sinensis l. osbeck), and rough lemon (rl) (citrus jambhiri lush). the changes in pa were compared to the amount of free proline, percent wood kill an ...198716665504
[a study on the quality of fruits of citrus aurantium l. (zhishi and zhiqiao) produced in jiangxi].through a study on the quality of different commercial fruits of citrus aurantium (zhishi and zhiqiao) produced in jiangxi, it has been found that the quality of eyan zhishi is good and jiang zhishi and jiang zhiqiao are the best varieties.19892511867
protoplast-fusion-mediated transfer of organelles from microcitrus into citrus and regeneration of novel alloplasmic trees.iodoacetate-treated citrus protoplasts from embryogenic nucellar calli of sour orange (c. aurantium) or from rough lemon (c. jambhiri) were fused with γ-irradiated protoplasts from a related genus, microcitrus. the fused protoplasts were cultured to obtain colonies and micro-calli. micro-calli derived from these two fusion combinations were isolated, propagated and differentiated into embryos, which subsequently regenerated trees having the morphology of sour orange or rough lemon. these interge ...198924225837
induction of anti-orange antibodies in rabbits.the capacity of certain foods to cause allergic reactions is well known. the four types of mechanisms that gell and coombs described in 1968, are involved in these reactions, although the reaginic antibodies retain the paramount attention of the immunologists. the physiochemical composition of the allergen molecule is the goal of investigators with the purpose to clarify the intrinsic kinetics of antibody synthesis. this paper contributes to the conflicting data about orange allergens especially ...19892635832
management of clinical cases of peste des petits ruminants (ppr) disease in goats.clinical cases of peste des petits ruminants (ppr) disease in goats were treated symptomatically. broad spectrum antibiotics, intestinal sedatives and fluid therapy were used for the treatment of pneumonia, diarrhoea and the restoration of the body fluid ionic balance. good feeding and nursing in warm, draught-free pens are necessary. lemon fruit and citrus aurantium proved to be effective for the treatment of the orf-like labial scabs. this treatment raised the survival rate of goats by 13.3%.19892619704
host status of citrus and citrus relatives to tylenchulus graminis.studies were conducted on the host suitability of four citrus rootstocks--rough lemon (citrus limon), sour orange (c. aurantium), trifoliate orange (poncirus trifoliata cv. argentina), and swingle citrumelo (c. paradisi x p. trifoliata)--to tylenchulus graminis which was previously considered a "grass" race of t. semipenetrans. in an uncultivated field, sour orange seedlings grown with t. graminis-infected broomsedge (andropogon virginicus) were not infected with this nematode after 18-month's e ...198919287668
effect of fenamiphos placement on tylenchulus semipenetrans and yield in a florida citrus orchard.grapefruit trees on sour orange rootstock on the east coast of florida were treated with 22.3 kg a.i./ha fenamiphos (broadcast equivalent) in 1.52-m bands extending from the dripline to beneath the canopy, in 1.52-m bands extending from the dripline toward the row middle, or left as untreated controls. during the course of the experiment, mean density of feeder roots and tylenchulus semipenetrans in control plots was 3.8 and 5.8 times greater, respectively, in samples from beneath tree canopies ...198919287676
efficient procedure for extracting tylenchulus semipenetrans from citrus roots.investigations were undertaken to determine the suitability of sucrose and magnesium sulphate solutions and a silica colloidal suspension with centrifugation for extracting tylenchulus semipenetrans from citrus roots. the efficiency of incubation, sodium hypochlorite, centrifugation, and maceration methods was also compared. numbers of females recovered by centrifugation with colloidal silica were greater than those from sucrose or magnesium sulphate. incubation, sodium hypochlorite, and centrif ...199019287763
influence of vesicular arbuscular mycorrhizae and leaf age on net gas exchange of citrus leaves.the purpose of this study was to test the hypothesis that vesicular arbuscular mycorrhizal (vam) fungi affect net assimilation of co(2) (a) of different-aged citrus leaves independent of mineral nutrition effects of mycorrhizae. citrus aurantium l., sour orange plants were grown for 6 months in a sandy soil low in phosphorus that was either infested with the vam fungus, glomus intraradices schenck & smith, or fertilized with additional phosphorus and left nonmycorrhizal (nm). net co(2) assimilat ...199016667848
movement of cations through cuticles of citrus aurantium and acer saccharum: diffusion potentials in mixed salt solutions.we examined some biophysical mechanisms of ion migration across leaf cuticles enzymatically isolated from acer saccharum l. and citrus aurantium l. leaves. diffusion potential measurements were used to calculate the permeabilities of cl(-), li(+), na(+), and cs(+) ions all as a ratio with respect to the permeability of k(+) in cuticles. in 2 millimolar ionic strength solutions the permeability sequence from high to low was k = cs > na > li > cl. when the outer and inner surfaces of cuticles were ...199016667677
the effect of the environment on the permeability and composition of citrus leaf cuticles : ii. composition of soluble cuticular lipids and correlation with transport properties.the constituents of the soluble cuticular lipids (scl) of the leaf blades of citrus aurantium l. were identified by gas chromatography-mass spectrometry and quantified. major components were 1-alkanols (c24 to c40), n-alkyl esters (c36 to c56), n-alkanoic acids (c28 to c34), n-alkanes (c22 to c40) and triterpenones, while n-alkanals (c29 to c38), sterols, and alkyl benzenes (molecular weights 260, 274 and 288) made minor contributions. leaf age and side significantly affected the quantitative co ...199024201939
the effect of the environment on the permeability and composition of citrus leaf cuticles : i. water permeability of isolated cuticular membranes.the water permeabilities of 3174 astomatous, isolated cuticular membranes from citrus aurantium l. leaves were studied. trees were grown in environmental chambers at temperatures ranging from 15 to 35° c and humidities of 50% and 90%. photosynthetically active radiation was 500-1000 μmol photons·m(-2)· s(-1). the different growing conditions had no effect on the water permeability of the membranes. however, storing isolated cuticular membranes at 8° c for up to 112 weeks decreased their water pe ...199024201938
leaf cuticles behave as asymmetric membranes : evidence from the measurement of diffusion potentials.cuticles were isolated enzymatically from the leaves of two maple species (acer saccharum marsh and a. platanoides l.) and from orange (citrus aurantium l.). the cuticles were placed in a plastic cuvette and different concentrations of kcl were perfused over the physiological inner and outer surfaces while the electrical potential (e(10)) that developed across the cuticles and was caused by ion diffusion was measured. e(10) was always positive, indicating that the permeability of k(+) was always ...199016667229
accumulation of the sesquiterpenes nootkatone and valencene by callus cultures of citrus paradisi, citrus limonia and citrus aurantium.the production of the sesquiterpenes nootkatone and valencene by callus cultures of citrus species is described. the levels of these compounds were examined by gas chromatography-mass spectrometry and their yields were compared with the amounts found in mature fruits. a simultaneous increase and decrease in the levels of nootkatone and valencene, respectively, were observed with the aging of callus cultures of citrus paradisi. these results suggest that valencene might be a possible precursor of ...199124221735
diffusion and electric mobility of ions within isolated cuticles of citrus aurantium: steady-state and equilibrium values.we report a new method for measuring cation and anion permeability across cuticles of sour orange, citrus aurantium, leaves. the method requires the measurement of two electrical parameters: the diffusion potential arising when the two sides of the cuticle are bathed in unequal concentrations of a cl(-) salt; and the electrical conductance of the cuticle measured at a salt concentration equal to the average of that used in the diffusion-potential measurement. the permeabilities of h(+), li(+), n ...199116668382
downward regulation of photosynthesis and growth at high co(2) levels : no evidence for either phenomenon in three-year study of sour orange trees.numerous photosynthesis and growth measurements of sour orange (citrus aurantium l.) trees maintained in ambient air and air enriched with an extra 300 microliters per liter of co(2) have revealed the co(2)-enriched trees to have consistently sequestered approximately 2.8 times more carbon than the control trees over a period of three full years. under field conditions in the natural environment, plants may not experience the downward regulation of photosynthetic capacity typically observed in l ...199116668286
estimation of mesophyll conductance to co(2) flux by three different methods.the resistance to diffusion of co(2) from the intercellular airspaces within the leaf through the mesophyll to the sites of carboxylation during photosynthesis was measured using three different techniques. the three techniques include a method based on discrimination against the heavy stable isotope of carbon, (13)c, and two modeling methods. the methods rely upon different assumptions, but the estimates of mesophyll conductance were similar with all three methods. the mesophyll conductance of ...199216668812
bioproduction of neohesperidin and naringin in callus cultures of citrus aurantium.the accumulation of both neohesperidin and naringin as major flavonoids in callus cultures of bitter orange (citrus aurantium) was demonstrated using high performance liquid chromatography with a diode-array detector. the identity of both compounds was confirmed by their corresponding nuclear magnetic resonance spectra. the levels of neohesperidin are higher than those of naringin in callus culture, as they are in immature fruit, and high concentrations of both are found in young tissues such as ...199224213293
diffusion and electric mobility of kci within isolated cuticles of citrus aurantium.fick's second law has been used to predict the time course of electrical conductance change in isolated cuticles following the rapid change in bathing solution (kci) from concentration c to 0.1 c. the theoretical time course is dependent on the coefficient of diffusion of kci in the cuticle and the cuticle thickness. experimental results, obtained from cuticles isolated from sour orange (citrus aurantium), fit with a diffusion model of an isolated cuticle in which about 90% of the conductance ch ...199216668971
naringin and neohesperidin levels during development of leaves, flower buds, and fruits of citrus aurantium.the distribution of the flavanones naringin and neohesperidin has been analyzed during the development of the leaves, flower buds, and fruits of citrus aurantium. these flavonoids are at maximum concentration in the organs studied during the logarithmic phase of growth, gradually decreasing until the organs reach maximum development. however, this decrease in the naringin and neohesperidin concentration in leaves, flower buds, and fruits is due to a dilution of the flavonoids caused by cell grow ...199216668885
effects of atmospheric co(2) enrichment on photosynthesis, respiration, and growth of sour orange trees.numerous net photosynthetic and dark respiratory measurements were made over a period of 4 years on leaves of 24 sour orange (citrus aurantium) trees; 8 of them growing in ambient air at a mean co(2) concentration of 400 microliters per liter, and 16 growing in air enriched with co(2) to concentrations approaching 1000 microliters per liter. over this co(2) concentration range, net photosynthesis increased linearly with co(2) by more than 200%, whereas dark respiration decreased linearly to only ...199216668873
suppression of somatic embryogenesis in citrus cell cultures by extracellular proteins.nucellar-derived cell cultures of sour orange (citrus aurantium l.) proliferate as proembryogenic masses. by a change in the carbon source of the medium from sucrose to glycerol they are induced to undergo synchronous embryogenesis forming embryo initials that develop into globular embryos. the proembryogenic masses released glycoproteins to the medium. exogenous addition of the glycoproteins to cells in glycerol-containing medium modified the course of embryo development in a dose-dependent man ...199224186780
citrus peel oil extracts as mosquito larvae insecticides.susceptibility tests were carried out in culex quinquefasciatus larvae using peel oil extracts of bitter orange (citrus aurantium), orange (citrus sinensis) and lemon (citrus limon). larvae mortalities were observed indicating that the extracts may contain potentially useful insecticides.19921644035
host range of a population of pratylenchus vulnus in commercial fruit, nut, citrus, and grape rootstocks in spain.in a host-range study carried out under greenhouse conditions, a total of 37 commercial fruit tree, grape, and citrus rootstocks were tested for their reaction to a population of the lesion nematode, pratylenchus vulnus, in spain. twenty-five rootstocks had a pf/pi > 1.5. these included almond (desmayo rojo, 1143), apple (em-9, em-106), avocado (hass), cherry (santa lucia 64, camil, m x m 14, masto de montafiana), grape (41-b, fercal, ritcher 110), hazelnut (pauetet), loquat (nadal), peach (mont ...199219283047
nucleotide sequence of the coat protein gene of citrus tristeza virus: comparison of biologically diverse isolates collected in israel.the sequences of the coat protein genes of four seedling yellows (sy) and four non-sy (nsy) of citrus tristeza virus (ctv) isolates, which were collected in israel over a period of 30 years, were analyzed. pairwise comparisons showed extensive similarities in the nucleotide and amino acid sequences of six isolates designated the vt group. this group consists of three nsy isolates that cause a very mild ctv reaction on the sensitive combination of sweet orange (swo) grafted on sour orange (so), a ...19938279125
column liquid chromatographic determination of flavanone glycosides in citrus. application to grapefruit and sour orange juice adulterations.six flavone glycosides were separated by hplc using rp-18 with a quaternary mobile phase mixture [water-acetonitrile-tetrahydrofuran-acetic acid (80:16:3:1, v/v)]. this procedure was applied to the separation and determination of the most important flavanone glycosides contained in pure grapefruit and sour orange juices and mixtures. this method can be used for quality control of industrial concentrates and juices.19938486744
reproductive fitness and random amplified polymorphic dna variation among isolates of pratylenchus vulnus.the reproductive fitness of seven isolates of pratylenchus vulnus from different geographical areas and hosts was assessed in monoxenic cultures (carrot), and greenhouse cultures (plum, sour orange, and quince). the genetic makeup of the different isolates was compared by random amplified polymorphic dna (rapd-pcr). the apple (pvap-s) and apricot (pvat-f) isolates reproduced less in monoxenic cultures than the rose (pvro-s) and walnut (pvwa-a and pvwa-u) isolates. on plum, the rose isolate (pvro ...199419279892
fructus aurantii reduced portal pressure in portal hypertensive rats.the purpose of this study was to investigate the effects of fructus aurantii (the unripe fruits of citrus aurantium l.) on portal hypertensive rats. portal hypertension was induced by partial portal vein ligation (pvl) in sprague-dawley rats. sham-operated (sham) rats served as controls. hemodynamic and in vitro contractile studies were performed at 14 days after surgery. both the aqueous extract of fructus aurantii and synephrine, one of its purified principles with pressor activity, were infus ...19957475952
naringin and naringenin are not the primary cyp3a inhibitors in grapefruit juice.the effect of various citrus juices and solutions of naringin on cyp3a activity in rat liver microsomes was compared by measuring the formation of 6 beta-hydroxytestosterone from testosterone. control enzyme activity was reduced by more than 70% by grapefruit juice. freshly-squeezed sour (seville) orange juice containing 20% as much naringin was comparable to grapefruit juice in its ability to inhibit microsomal activity. an aqueous solution of naringin at the same concentration as in grapefruit ...19968809221
[studies on the bioactive constituents of aurantii fructus immaturus].an ethanol extract of "kijitsu" (aurantii fructus immaturus, citrus aurantium l.) collected in china was assessed for the antitumor activity using murine leukemia p388 in vivo, and the extract was found to be active by the antitumor bioassay in vivo and in vitro. the extract was separated into a petroleum ether-soluble fraction and an ethyl acetate-soluble fraction. fractionation was carried out using an index of cell-growth inhibitory activity against mouse leukemia l1210 cells to isolate antit ...19968721353
carbon economy of sour orange in response to different glomus spp.vesicular-arbuscular mycorrhizal (m) fungal colonization, growth, and nonstructural carbohydrate status of sour orange (citrus aurantium l.) seedlings were compared at low- and high-phosphorus (p) supply following inoculation with four glomus isolates: g. intraradices (gi, fl208), g. etunicatum (ge, ut316), g. claroideum (gc, sc186), and glomus sp. (g329, fl906). nonmycorrhizal (nm) seedlings served as controls. at low-p supply, increases in incidence of m colonization, vesicles and accumulation ...199614871797
oil of bitter orange: new topical antifungal agent.superficial dermatophyte infection is one of the most common dermatologic diseases. some of these infections are extremely resistant to therapy.19968737885
[textual studies on shangzhou zhiqiao fructus aurantii].the original plant of shangzhou zhiqiao, the best among the species of zhiqino, is confirmed as citrus aurantium rather than poncirus trifolia in this paper based on the description and drawings of zhiqiao in (bencao gangmu) by li shizhen in the 16th rentury, samples from shangzhou(shanxi province), and the cold-warm period of historical climate in the local area, which is believed to be related to the distribution of c. aurantium in shangzhou. however, p.trifolia has often been confused with c. ...19969206251
further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion.somatic hybridization experiments in citrus that involve the fusion of protoplasts of one parent isolated from either nucellus-derived embryogenic callus or suspension cultures with leaf-derived protoplasts of a second parent, often result in the regeneration of diploid plants that phenotypically resemble the leaf parent. in this study, plants of this type regenerated following somatic fusions of the following three parental combinations were analyzed to determine their genetic origin (nuclear a ...199624178608
analysis of bitter essential oils from orange and grapefruit by high-performance liquid chromatography with microbore columns.the analysis of bitter orange and grapefruit essential oils (non-volatile fraction) was carried out by hplc in normal- and reversed-phase mode with uv detection. these oils were compared with the sweet orange and mandarin essential oils, analyzed previously. for the identification of chromatographic peaks, fractionation by rp-hplc was carried out. the purified fractions were analyzed by gc-ms and lc-ms. some new compounds were found, together with many others already identified in different citr ...19968680599
citrus response to salinity: growth and nutrient uptake.to determine the effects of salinity on relative growth rate (rgr), net assimilation rate on a leaf weight basis (nar(w)), leaf weight ratio (lwr), and nutrient uptake and utilization of citrus, we grew four citrus rootstocks (sour orange, cleopatra mandarin, carrizo citrange and citrus macrophylla) in nutrient solutions containing 0, 10, 20, 40 or 80 mm nacl for 20, 40 or 60 days. for each element analyzed, specific absorption rate (sar) and specific utilization rate on a leaf basis (sur(l)) we ...199714759868
biological screening of 100 plant extracts for cosmetic use (ii): anti-oxidative activity and free radical scavenging activity.methanol aqueous extracts of 100 plants were screened for anti-oxidative activity using fenton's reagent/ethyl linoleate system and for free radical scavenging activity using the 1,1-diphenyl-2-picryl hydrazyl free radical generating system. the results suggest that 14 plants - alpinia officinarum, areca catechu, brassica alba, cannabis sativa, curcuma longa, curcuma aromatica, eugenia caryophyllata, evodia officinalis, paeonia suffruticosa, rhaphanus sativus, rheum palmatum, rhus verniciflua, t ...199718505484
relationship between volatile components of citrus fruit essential oils and antimicrobial action on penicillium digitatum and penicillium italicum.this study examined the effect of volatile components of citrus fruit essential oils on p. digitatum and p. italicum growth. the hydrodistilled essential oils of orange (citrus sinensis cvv. "washington navel", "sanguinello", "tarocco", "moro", "valencia late", and "ovale"), bitter (sour) orange (c. aurantium), mandarin (c. deliciosa cv. "avana"), grapefruit (c. paradisi cvv. "marsh seedless" and "red blush"), citrange (c. sinensis x poncirus trifoliata cvv. "carrizo" and "troyer"), and lemon (c ...19989761340
histological comparison of fibrous root infection of disease-tolerant and susceptible citrus hosts by phytophthora nicotianae and p. palmivora.abstract phytophthora nicotianae and p. palmivora infect and cause rot of fibrous roots of susceptible and tolerant citrus rootstocks in florida orchards. the infection and colonization by the two phytophthora spp. of a susceptible citrus host, sour orange (citrus aurantium), and a tolerant host, trifoliate orange (poncirus trifoliata), were compared using light and electron microscopy. penetration by both phytophthora spp. occurred within 1 h after inoculation, regardless of the host species. n ...199818944916
[studies on chinese drugs zhiqiao and zhishi origin investigation and merchandise identification].to sort out the main original plants of chinese drugs zhiqiao and zhishi.199912205880
6',7'-dihydroxybergamottin in grapefruit juice and seville orange juice: effects on cyclosporine disposition, enterocyte cyp3a4, and p-glycoprotein.6',7'-dihydroxybergamottin is a furanocoumarin that inhibits cyp3a4 and is found in grapefruit juice and seville orange juice. grapefruit juice increases the oral bioavailability of many cyp3a4 substrates, including cyclosporine (inn, ciclosporin), but intestinal p-glycoprotein may be a more important determinant of cyclosporine availability.199910096255
d-rna molecules associated with subisolates of the vt strain of citrus tristeza virus which induce different seedling-yellows reactions.citrus tristeza virus (ctv) strains were previously catalogued as seedling-yellows (sy) and non-sy (nsy) types, according to their yellowing and stunting effects on indicator seedlings. among subisolates of the vt strain, which were selected from chronically infected alemow plants, there was a correlation between the presence of 2.4-, 2.7- and 4.5-kb d-rnas, and sy and nsy reactions, respectively. similarly, plants infected with mor-t subisolates, which cause sy, contained d-rnas of 2.6 to 2.8 k ...199910499445
antimutagenic activity of polymethoxyflavonoids from citrus aurantium.the methanol extract from citrus aurantium showed a suppressive effect on umu gene expression of sos response in salmonella typhimurium ta1535/psk1002 against the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide). the methanol extract from c. aurantium was successively re-extracted with hexane, dichloromethane, butanol, and water. a dichloromethane fraction showed a suppressive effect. the suppressive compounds in the dichloromethane fraction were isolated by sio(2) column chroma ...199910606602
[clinical study on retarding aging effect of tongbu recipe to traditional chinese medicine].to study the mechanism of tongbu no. 1 (tb1, a prescription for reinforcing kidney and spleen, clearing up the bowel viscera to send turbid downward and regulating qi and blood) in retarding aging.199911783270
[standardization of the sour orange flower and leaf].dried flowers (1 batch) and leaves (6 batches) of sour orange citrus aurantium l. had a similar flavonoid pattern. but the flavonoid levels of flowers were higher than those of leaves. the mean levels of the principal flavonoid compounds were respectively: total flavonoids 12.35 and 1.06%, neohesperidin 5.44 and 0.08%, naringin 1.93 and 0.06%, eriocitrin 0.38 and 0.25%. 18 batches of commercial origine were also examined for a comparative study. specifications were proposed for a revision of the ...199910520514
fast hplc for the analysis of oxygen heterocyclic compounds of citrus essential oils.a fast hplc method for the determination of the oxygen heterocyclic compounds of citrus essential oils was developed. five different oils were analyzed under identical conditions, by reversed-phase hplc with photodiode array detector, for a direct comparison of the composition of their oxygen heterocyclic fraction. analysis time was 7 min. the oils analyzed were lemon, bergamot, mandarin, sweet orange, and bitter orange. the method developed is good for rapid screening or fingerprinting of these ...199910552795
lc-ms for the identification of oxygen heterocyclic compounds in citrus essential oils.the oxygen heterocyclic compounds (coumarins, psoralens and polymethoxylated flavones) present in the nonvolatile residue of the essential oils of mandarin, sweet orange, bitter orange, bergamot and grapefruit were analysed with an hplc/api/ms system equipped with an apci probe in positive mode. the use of hyphenated techniques, such as lc/ms provides a great information about the content and nature of constituents of natural complex matrices, such as essential oils. in this work, ms spectra wer ...200011108548
[production of intergeneric somatic hybrid plants via protoplast electrofusion in citrus].leaf derived protoplasts of sour orange (citrus aurantium l.) were fused electrically with embryogenic protoplasts of microcitrus papuana swingle. plants were regenerated from the fusion products, which were characteristic with three types of leaf morphology. most of the plants were identical to sour orange (namely, leaf-parent-type plant) and two plants had large and thick leaves whereas one plant had bifoliate and trifoliate leaves. chromosome examination showed that these plants were diploid ...200012549071
[two interspecific somatic hybrid plants regenerated via protoplast electro-fusion].protoplasts isolated from cell suspension cultures of 'bonnaza' navel orange (citrus sinensis l. osbeck) were electrically fused with mesophyll protoplasts of rough lemon (citrus jambhiri lush) and goutou orange (citrus aurantium l.) respectively. plants regenerated from both fusion combinations. chromosome counting of randomly selected fifty two globular embryoids as well as all the regenerated seventy four plants from bonnaza navel + rough lemon revealed that twenty six embryoids were tetraplo ...200010976322
obesity and medicinal plants.the effects of some medicinal plants (i.e. fucus vesiculosus, citrus aurantium) that are claimed to be useful in the treatment of obesity are reviewed.200010930716
[determination of naringin and synephrine in fructus aurantii from different habitats by hplc].this paper reports the determination of naringin and synephrine in fructus aurantii from different habitats by hplc. the result shows the highest content of naringin in citrus aurantium l. 'xiangcheng' from genuine crude drug habitat xingan city jiangxi provice, the highest content of synephrine in citrus aurantium l. 'xiangcheng' from yiyang city jiangxi provice. quality evaluation of genuine crude drug was studied preliminarily.200012575153
high efficiency genetic transformation of sour orange (citrus aurantium) and production of transgenic trees containing the coat protein gene of citrus tristeza virus.in preliminary experiments on agrobacterium-mediated transformation of citrus, we found transformation events occurring in callus formed from the cambium. factors affecting agrobacterium-sour orange (citrus aurantium l.) interactions, such as culture medium, explant source and culture conditions, were studied to assess competence for transformation in such callus and to improve transformation frequency. cell divisions and redifferentiation from the transgenic cells leading to transformation even ...200012651494
biology of diaphorina citri (homoptera: psyllidae) on four host plants.the biology of the citrus psyllid diaphorina citri kuwayama was studied at 25 degrees c on four commonly grown citrus and related plants [rough lemon, citrus jambhiri lush; sour orange, c aurantium l.; grapefruit, c. paradisi macfadyen; and orange jessamine, murraya paniculata (l.) jack] in the laboratory. the biological characteristics of each life stage are described. the average egg incubation periods on orange jessamine, grapefruit, rough lemon, and sour orange varied very little (4.1-4.2 d) ...200011142304
incidence and epidemiology of citrus tristeza virus in the valencian community of spain.the first outbreak of citrus tristeza disease in spain caused by citrus tristeza virus (ctv) was recorded in 1957 in the valencian community (vc). in total c. 40 million trees, mainly of sweet orange and mandarin grafted on sour orange rootstocks, declined due to ctv. large-scale surveys in different municipalities of the vc indicated that the disease spread very fast. incidence increased from 11% in 1989 to 53% in 1998. toxoptera aurantii and aphis spiraecola (inefficient aphid vectors of ctv) ...200011137164
acute intoxication of cyclosporin caused by coadministration of decoctions of the fruits of citrus aurantium and the pericarps of citrus grandis.in order to investigate the effects of citrus herbs on cyclosporin absorption and disposition, swine were given cyclosporin (10 mg/kg) with or without decoctions of citri aurantii fructus (caf) or citri grandis pericarpium (cgp) in a crossover design. fpia method (fluorescence polarization immunoassay) was used to determine the blood concentration of cyclosporin. the decoctions were characterized by their flavanone contents. our results indicated that the coadministration of caf and cgp signific ...200011105574
role of furanocoumarin derivatives on grapefruit juice-mediated inhibition of human cyp3a activity.with juices of grapefruit and related fruits, possible relationships between contents of six different furanocoumarins and extents of inhibition of microsomal cyp3a activity have been studied in vitro. microsomal cyp3a-mediated testosterone 6beta-hydroxylation was inhibited by the addition of a fruit juice (2.5%, v/v) from eight different grapefruit sources, two sweeties, three pomelos, and one sour orange, whereas no clear inhibition was observed with two sweet orange juices. the inhibitory com ...200010859150
inhibitory effect of herbal medicines on rotavirus infectivity.the inhibitory effect of some traditional herbal medicines on the infectivity of rotavirus, which predominantly occurs in sporadic diarrhea in infants and young children, was investigated. among the 34 kinds of herbal medicines tested, the fruit of citrus aurantium had the most potent inhibitory activity on rotavirus infection. the active components of the fruit of citrus aurantium were neohesperidin and hesperidin. their 50% inhibitory concentrations were 25 and 10 microm, respectively.200010726895
seville orange juice-felodipine interaction: comparison with dilute grapefruit juice and involvement of furocoumarins.our objective was to determine whether seville orange juice produces a grapefruit juice-like interaction with felodipine and whether bergamottin, 6',7'-dihydroxybergamottin, or other furocoumarins are involved.200111180034
comparative study of the assay of artemia salina l. and the estimate of the medium lethal dose (ld50 value) in mice, to determine oral acute toxicity of plant extracts.artemia salina l. (artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemical and natural products. in this study the medium lethal concentrations (lc50 value) of 20 plant extracts, aloe vera (l.) burm. f. (aloeaceae), artemisia absinthium l. (asteraceae); citrus aurantium l. (rutaceae); cymbopogon citratus (dc. ex nees) stapf (poaceae); datura stramonium l. (solanaceae); justicia pectoralis jacq. (acanthaceae); musa x paradisiaca l. (m ...200111695884
toxic effects of six plant oils alone and in combination with controlled atmosphere on liposcelis bostrychophila (psocoptera: liposcelididae).six plant essential oils alone as repellent and fumigant, and in combination with the controlled atmosphere against liposcelis bostrychophila badonnel were assessed in the laboratory. these essential oils were extracted from the leaves of six source plants: citrus tangerina tanaka, citrus aurantium l., citrus bergamia risso et poiteau, pinus sylvestris l., cupressus funebris end]., and eucalyptus citriodora hook. the repellency test indicated that l. bostrychophila adults were repelled by filter ...200111681697
inhibitory potential of herbal medicines on human cytochrome p450-mediated oxidation: properties of umbelliferous or citrus crude drugs and their relative prescriptions.to investigate the possible drug interaction with herbal medicine, hot water decoctions or 40% ethanol infusions of several umbelliferous or citrus crude drugs and their prescriptions were examined in vitro for their abilities to inhibit human cytochrome p450 3a (cyp3a). addition of each decoction or infusion from baizhi (angelica dahurica and varieties), qianghuo (notopterygium incisum or n. forbesii), duhuo (angelica biserrata), fangfeng (saposhnikovia divaricata), danggui (angelicasinensis), ...200111388644
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