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bioassay-based isolation and identification of phenolics from sweet cherry that promote active glucose consumption by hepg2 cells.a variety of phenolics had been found to be functional in promoting cellular glucose consumption that is important for blood glucose regulation. sweet cherry (prunus avium) is rich in such kinds of phenolics, including hydrocinnamic acids, anthocyanins, flavonols, and flavan-3-ols. furthermore, a sweet cherry phenolics-rich extract (pre) was found to be effective in promoting hepg2 glucose consumption. seventeen components were preliminarily identified by hplc-esi-ms, including 9 hydrocinnamic a ...201525559482
effect of β-aminobutyric acid on cell wall modification and senescence in sweet cherry during storage at 20°c.the effects of postharvest β-aminobutyric acid (baba) treatment on fruit firmness, pectin degrading enzymes, cell wall constituents and microstructural alterations of pericarp in sweet cherry fruit were investigated. baba significantly delayed the decline of fruit firmness and inhibited the increase of membrane permeability and the accumulation of malondialdehyde in cherries. the baba-treated fruit exhibited significantly higher contents of water-soluble pectin, cdta-soluble pectin, na2co3-solub ...201525577108
association between chloroplast and mitochondrial dna sequences in chinese prunus genotypes (prunus persica, prunus domestica, and prunus avium).the nuclear dna is conventionally used to assess the diversity and relatedness among different species, but variations at the dna genome level has also been used to study the relationship among different organisms. in most species, mitochondrial and chloroplast genomes are inherited maternally; therefore it is anticipated that organelle dna remains completely associated. many research studies were conducted simultaneously on organelle genome. the objectives of this study was to analyze the genet ...201525592231
characterization of four popular sweet cherry cultivars grown in greece by volatile compound and physicochemical data analysis and sensory evaluation.volatile compounds, physicochemical and sensory attributes of four sweet cherry cultivars (canada giant, ferrovia, lapins and skeena) grown in northern greece were determined. eighteen volatile compounds were identified and semi-quantified in cherries using solid phase micro extraction in combination with gas chromatography/mass spectrometry (spme-gc/ms). carbonyl compounds were the most abundant in sweet cherry aroma, followed by alcohols, esters and hydrocarbons/terpenes. cherry cultivars in o ...201525629454
physicochemical characteristics, antioxidant activity, organic acid and sugar contents of 12 sweet cherry (prunus avium l.) cultivars grown in turkey.physical characteristics, antioxidant activity and chemical constituents of 12 cultivars (prunus avium l.) of sweet cherry (belge, bing, dalbasti, durona di cesena, lambert, merton late, starks gold, summit, sweetheart, van, vista, and 0-900 ziraat) were investigated. significant differences (p < 0.05) were observed among tested cultivars for ph, total soluble solid, hardness, color parameters, antioxidant activities and pomological measurements (p < 0.05). the color parameters were important to ...201525631389
genetic diversity in the coat protein genes of prune dwarf virus isolates from sweet cherry growing in turkey.sweet cherry is an important fruit crop with increasing economical value in turkey and the world. a number of viruses cause diseases and economical losses in sweet cherry. prune dwarf virus (pdv), is one of the most common viruses of stone fruits including sweet cherry in the world. in this study, pdv was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of isparta province of turkey by double antibody sandwich-enzyme linked immunosorbent assay (das-elisa) ...201525774109
physiological and biochemical changes relating to postharvest splitting of sweet cherries affected by calcium application in hydrocooling water.hydrocooling sweet cherries shortly after harvest (4h) and then transporting fruit in cold flume water during packing are used to maximize postharvest quality, but can cause fruit splitting. this study demonstrated that cherry fruit (two splitting-susceptible cultivars) absorbed ca in a quadratic polynomial manner with increasing cacl2 concentration from 0.2% to 2.0% in cold water (0°c) for 5 min, but did not take up cl. the enhanced tissue ca content reduced splitting potential by decreasing fr ...201525794746
comparative transcriptome analysis of genes involved in anthocyanin biosynthesis in the red and yellow fruits of sweet cherry (prunus avium l.).fruit color is one of the most important economic traits of the sweet cherry (prunus avium l.). the red coloration of sweet cherry fruit is mainly attributed to anthocyanins. however, limited information is available regarding the molecular mechanisms underlying anthocyanin biosynthesis and its regulation in sweet cherry.201525799516
effect of pulsed electric fields on the flavour profile of red-fleshed sweet cherries (prunus avium var. stella).the aim of this research was to study the effect of pulsed electric fields (pef) on the flavour profile of red-fleshed sweet cherries (prunus avium variety stella). the cherry samples were treated at a constant pulse frequency of 100 hz, a constant pulse width of 20 μs, different electric field strengths between 0.3 and 2.5 kv/cm and specific energy ranging from 31 to 55 kj/kg. volatile compounds of samples were analysed using an automated headspace solid phase microextraction (hs-spme) method c ...201525806548
breaking seed dormancy of three orthodox mediterranean rosaceae species.biodiversity levels could be enhanced when regenerating a site by seed-derived seedlings. however, seed dormancy poses limitations for many species. as a result, nurseries either produce seedlings from species where dormancy is not an obstacle, or they propagate through cuttings with the risk of decreasing the genetic diversity within and among species at the regenerated sites. in the present study, breaking of seed dormancy was investigated in valuable mediterranean species of prunus avium, pru ...201525895254
paccyp707a2 negatively regulates cherry fruit ripening while paccyp707a1 mediates drought tolerance.sweet cherry is a non-climacteric fruit and its ripening is regulated by abscisic acid (aba) during fruit development. in this study, four cdnas (paccyp707a1-4) encoding 8'-hydroxylase, a key enzyme in the oxidative catabolism of aba, were identified in sweet cherry fruits using tobacco rattle virus-induced gene silencing (vigs) and particle bombardment approaches. quantitative real-time pcr confirmed significant down-regulation of target gene transcripts in vigs-treated cherry fruits. in paccyp ...201525956880
expression and anthocyanin biosynthesis-modulating potential of sweet cherry (prunus avium l.) myb10 and bhlh genes.anthocyanins are essential contributors to fruit coloration, an important quality feature and a breed determining trait of a sweet cherry fruit. it is well established that the biosynthesis of anthocyanins is regulated by an interplay of specific transcription factors belonging to myb and bhlh families accompanied by a wd40 protein. in this study, we isolated and analyzed pawd40, pabhlh3, pabhlh33, and several closely related myb10 gene variants from different cultivars of sweet cherry, analyzed ...201525978735
construction of high density sweet cherry (prunus avium l.) linkage maps using microsatellite markers and snps detected by genotyping-by-sequencing (gbs).linkage maps are valuable tools in genetic and genomic studies. for sweet cherry, linkage maps have been constructed using mainly microsatellite markers (ssrs) and, recently, using single nucleotide polymorphism markers (snps) from a cherry 6k snp array. genotyping-by-sequencing (gbs), a new methodology based on high-throughput sequencing, holds great promise for identification of high number of snps and construction of high density linkage maps. in this study, gbs was used to identify snps from ...201526011256
spring frost vulnerability of sweet cherries under controlled conditions.spring frost is a significant production hazard in nearly all temperate fruit-growing regions. sweet cherries are among the first fruit varieties starting their development in spring and therefore highly susceptible to late frost. temperatures at which injuries are likely to occur are widely published, but their origin and determination methods are not well documented. in this study, a standardized method was used to investigate critical frost temperatures for the sweet cherry cultivar 'summit' ...201626022603
physico-chemical properties and fatty acid composition of pomegranate, cherry and pumpkin seed oils.nut and seed oils are often considered waste products but in recent years they have been receiving growing interest due to their high concentration of hydrophilic and lipophilic bioactive components, which have important pharmacological properties on human health. the aim of this work was to compare the physico-chemical and biochemical properties of pomegranate (punicagranatum), sweet cherry (prunusavium) and pumpkin (cucurbita maxima) seed oils obtained by solvent extraction.201626033409
authentication of "cereza del jerte" sweet cherry varieties by free zone capillary electrophoresis (fzce).the purpose of this work was to develop a procedure based on protein analysis by free zone capillary electrophoresis (fzce) that can be used as an alternative to other methods in the determination of sweet cherry varieties for the authentication of "cereza del jerte". two autochthonous varieties of sweet cherry type "picota", 'ambrunés' and 'pico negro', and the foreign variety 'sweetheart' were used in the study. two protocols for extracting the methanol-soluble proteins were tested. on the bas ...200826047450
prunus transcription factors: breeding perspectives.many plant processes depend on differential gene expression, which is generally controlled by complex proteins called transcription factors (tfs). in peach, 1533 tfs have been identified, accounting for about 5.5% of the 27,852 protein-coding genes. these tfs are the reference for the rest of the prunus species. tf studies in prunus have been performed on the gene expression analysis of different agronomic traits, including control of the flowering process, fruit quality, and biotic and abiotic ...201526124770
mining microsatellites in the peach genome: development of new long-core ssr markers for genetic analyses in five prunus species.a wide inventory of molecular markers is nowadays available for individual fingerprinting. microsatellites, or simple sequence repeats (ssrs), play a relevant role due to their relatively ease of use, their abundance in the plant genomes, and their co-dominant nature, together with the availability of primer sequences in many important agricultural crops. microsatellites with long-core motifs are more easily scored and were adopted long ago in human genetics but they were developed only in few c ...201526185739
effect of nano-composite and thyme oil (tymus vulgaris l) coating on fruit quality of sweet cherry (takdaneh cv) during storage period.sweet cherry is one of the most appreciated fruit by consumers since it is an early season fruit and has an excellent quality. in this study effect of active nano composite formed from chitosan (as a matrix material), nano cellulose fiber (1% concentration) and thyme oils (tymus vulgaris l) at 1% concentration on fruits quality was investigated. treated fruits were stored at 1°c for 5 weeks and changes of different qualities attributes including weight loss, total acidity, tss, anthocyanin, tota ...026288725
chilling and host plant/site-associated eclosion times of western cherry fruit fly (diptera: tephritidae) and a host-specific parasitoid.the western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), is an endemic herbivore of bitter cherry, prunus emarginata (douglas ex hooker) eaton, but ∼100 years ago established on earlier-fruiting domesticated sweet cherry, prunus avium (l.) l. here, we determined if eclosion times of adult r. indifferens from sweet and bitter cherry differ according to the phenology of their respective host plants and if eclosion times of the host-specific parasitoid diachasma muliebre ...201526314048
prunetin signals via g-protein-coupled receptor, gpr30(gper1): stimulation of adenylyl cyclase and camp-mediated activation of mapk signaling induces runx2 expression in osteoblasts to promote bone regeneration.prunetin is found in red clover and fruit of prunus avium (red cherry). the effect of prunetin on osteoblast function, its mode of action and bone regeneration in vivo were investigated. cultures of primary osteoblasts, osteoblastic cell line and hek293t cells were used for various in vitro studies. adult female rats received drill-hole injury at the femur diaphysis to assess the bone regenerative effect of prunetin. prunetin at 10nm significantly (a) increased proliferation and differentiation ...201526345541
temperature-mediated kill and oviposition of western cherry fruit fly (diptera: tephritidae) in the presence of spinosad.western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), is a quarantine pest of sweet cherry (prunus avium (l.) l.) that is managed using insecticides, including spinosad, an organic compound that can be applied in low spray volumes. identifying factors that can increase the efficacy of spinosad can be useful for improving fly control. here, the major objective was to determine if temperature mediates kill and oviposition of r. indifferens in the presence of low spinosad ...201626352751
a study of operator performance for a mechanical sweet cherry harvester: comparison between manual and remote-controlled operation.an experimental mechanical sweet cherry harvester was upgraded by changing its rapid impact mechanism to a continuous impact mechanism, in which an end effector (impactor) is positioned on fruit-loaded branches to transfer vibrational energy for fruit removal, and by adding remote control capability. before the upgrade, the operator was seated at the rear of the harvester to manually control its operation. adding remote control capability allowed operator mobility while operating the machine. th ...201526373213
transcriptional dynamics of the developing sweet cherry (prunus avium l.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes.the exocarp, or skin, of fleshy fruit is a specialized tissue that protects the fruit, attracts seed dispersing fruit eaters, and has large economical relevance for fruit quality. development of the exocarp involves regulated activities of many genes. this research analyzed global gene expression in the exocarp of developing sweet cherry (prunus avium l., 'regina'), a fruit crop species with little public genomic resources. a catalog of transcript models (contigs) representing expressed genes wa ...201426504533
construction of commercial sweet cherry linkage maps and qtl analysis for trunk diameter.a cross between the sweet cherry (prunus avium) cultivars 'wanhongzhu' and 'lapins' was performed to create a mapping population suitable for the construction of a linkage map. the specific-locus amplified fragment (slaf) sequencing technique used as a single nucleotide polymorphism (snp) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (ssrs) and the incompatibility (s) gene, were used to build a map which comprised 8 linkage groups (lgs) a ...201526516760
[determination of anthocyanins in the peel of sweet cherry by ultra performance liquid chromatography tandem mass spectrometry].a method for the determination of seven anthocyanins in the peel of sweet cherry was developed by ultra performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms). the sample was extracted by methanol containing 0.1% (v/v) hydrochloric acid, and then purified by ab-8 macroporous resins. the separation was carried out on a phenomenex kinetex column (100 mm x 4.6 mm, 2.6 rm) with mobile phase of 0.1% (v/v) formic acid aqueous solution containing 5 mmol/l ammonium formate and methanol. ...201526536760
paternal-specific s-allele transmission in sweet cherry (prunus avium l.): the potential for sexual selection.homomorphic self-incompatibility is a well-studied example of a physiological process that is thought to increase population diversity and reduce the expression of inbreeding depression. whereas theoretical models predict the presence of a large number of s-haplotypes with equal frequencies at equilibrium, unequal allele frequencies have been repeatedly reported and attributed to sampling effects, population structure, demographic perturbation, sheltered deleterious mutations or selection pressu ...201626559165
mapping of candidate genes involved in bud dormancy and flowering time in sweet cherry (prunus avium).the timing of flowering in perennial plants is crucial for their survival in temperate climates and is regulated by the duration of bud dormancy. bud dormancy release and bud break depend on the perception of cumulative chilling during endodormancy and heat during the bud development. the objectives of this work were to identify candidate genes involved in dormancy and flowering processes in sweet cherry, their mapping in two mapping progenies 'regina' × 'garnet' and 'regina' × 'lapins', and to ...201526587668
(1)h nmr metabolic fingerprinting to probe temporal postharvest changes on qualitative attributes and phytochemical profile of sweet cherry fruit.sweet cherry fruits (prunus avium cvs. 'canada giant', 'ferrovia') were harvested at commercial maturity stage and analyzed at harvest and after maintenance at room temperature (storage at ∼20°c, shelf life) for 1, 2, 4, 6, and 8 days, respectively. fruit were initially analyzed for respiration rate, qualitative attributes and textural properties: 'canada giant' fruit were characterized by higher weight losses and stem browning index, being more intense over the late stages of shelf life period; ...201526617616
phenolic compounds, volatiles, and sensory characteristics of twelve sweet cherry (prunus avium l.) cultivars grown in turkey.the paper reports the phenolic, anthocyanin, and volatile compounds and sensory characteristics of 12 cultivars of sweet cherries including cvs. belge, bing, dalbasti, durona di cesena, lambert, merton late, starks gold, summit, sweetheart, van, vista, and 0-900 ziraat. eight individual phenolic compounds were determined by the hplc-dad method. among these cherries, cvs. bing, durona di cesena, and lambert contained higher levels of total individual phenolic compounds than the other cultivars. s ...201626646816
a single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry.a simple and rapid extraction procedure for the simultaneous determination of eight mycotoxins (alternaria toxins, ochratoxin a, patulin, citrinin) in a variety of fruit matrices has been developed using ultra high performance liquid chromatography coupled to tandem mass spectrometry. the procedure involves a one-step cleanup using homemade solid phase extraction (spe) cartridges. by comparative evaluation among six various adsorbents (c18, psa, hlb, mcx, silica, nh2), the combination of mcx and ...201626726936
complete nucleotide sequence of little cherry virus 1 (lchv-1) infecting sweet cherry in china.little cherry virus 1 (lchv-1), associated with little cherry disease (lcd), has a significant impact on fruit quality of infected sweet cherry trees. we report the full genome sequence of an isolate of lchv-1 from taian, china (lchv-1-ta), detected by small-rna deep sequencing and amplified by overlapping rt-pcr. the lchv-1-ta genome was 16,932 nt in length and contained nine open reading frames (orfs), with sequence identity at the overall genome level of 76%, 76%, and 78% to lchv-1 isolates y ...201626733294
effect of phytosanitary irradiation and methyl bromide fumigation on the physical, sensory, and microbiological quality of blueberries and sweet cherries.the objective of this study was to determine whether irradiation could serve as a suitable phytosanitary treatment alternative to methyl bromide (mb) fumigation for blueberries and sweet cherry and also to determine the effect of phytosanitary irradiation treatment on survival of salmonella spp. and listeria monocytogenes on these fruit. 'bluecrop' blueberries (vaccinium corymbosum) and 'sweetheart' cherries (prunus avium) were irradiated at 0.4 kgy or fumigated with methyl bromide and evaluated ...201626805024
changes in fine-root production, phenology and spatial distribution in response to n application in irrigated sweet cherry trees.factors regulating fine-root growth are poorly understood, particularly in fruit tree species. in this context, the effects of n addition on the temporal and spatial distribution of fine-root growth and on the fine-root turnover were assessed in irrigated sweet cherry trees. the influence of other exogenous and endogenous factors was also examined. the rhizotron technique was used to measure the length-based fine-root growth in trees fertilized at two n rates (0 and 60 kg ha(-1)), and the above- ...201626888890
genetic diversity, linkage disequilibrium, population structure and construction of a core collection of prunus avium l. landraces and bred cultivars.depiction of the genetic diversity, linkage disequilibrium (ld) and population structure is essential for the efficient organization and exploitation of genetic resources. the objectives of this study were to (i) to evaluate the genetic diversity and to detect the patterns of ld, (ii) to estimate the levels of population structure and (iii) to identify a 'core collection' suitable for association genetic studies in sweet cherry.201626912051
[effects of exogenous glucose and starch on soil carbon metabolism of root zone and root function in potted sweet cherry].one-year-old potted sweet cheery trees were treated with 4 g · kg(-1) exogenous glucose or starch and with non-addition of exogenous carbon as the control for up to 60 days. soil of root zone was sampled to analyze soil microbial biomass carbon, activities of invertase and amylase and microbial community functional diversity during the 60-day treatment, and roots were sampled for analysis of root respiratory rate, respiratory pathways and root viability after treatment for 30 days. results showe ...201526915183
impact of cultivar on profile and concentration of lipophilic bioactive compounds in kernel oils recovered from sweet cherry (prunus avium l.) by-products.lipophilic bioactive compounds in oils recovered from the kernels of seven sweet cherry (prunus avium l.) cultivars, harvested at single location in 2013, were studied. oil yield in sweet cherry ranged between 30.3-40.3 % (w/w) dw. the main fatty acids were oleic acid (39.62-49.92 %), linoleic acid (31.13-38.81 %), α-eleostearic acid (7.23-10.73 %) and palmitic acid (5.59-7.10 %), all four represented approximately 95 % of the total detected fatty acids. the ranges of total tocochromanols and st ...026984340
absolute quantification of pru av 2 in sweet cherry fruit by liquid chromatography/tandem mass spectrometry with the use of a stable isotope-labelled peptide.pru av 2, a pathogenesis-related (pr) protein present in the sweet cherry (prunus avium l.) fruit, is the principal allergen of cherry and one of the chief causes of pollen food syndrome (oral allergy syndrome). in this study, a quantitative assay for this protein was developed with the use of the protein absolute quantification (aqua) method, which consists of liquid chromatography/tandem mass spectrometry (lc/ms/ms) employing tgc[cam]stdasgk[(13)c6,(15)n2], a stable isotope-labelled internal s ...201626988485
turn-off fluorescence sensor for the detection of ferric ion in water using green synthesized n-doped carbon dots and its bio-imaging.this paper reports turn-off fluorescence sensor for fe(3+) ion in water using fluorescent n-doped carbon dots as a probe. a simple and efficient hydrothermal carbonization of prunus avium fruit extract for the synthesis of fluorescent nitrogen-doped carbon dots (n-cds) is described. this green approach proceeds quickly and provides good quality n-cds. the mean size of synthesized n-cds was approximately 7nm calculated from the high-resolution transmission electron microscopic images. x-ray photo ...201626994332
pararhizobium polonicum sp. nov. isolated from tumors on stone fruit rootstocks.five gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on different stone fruit rootstocks in poland: strains f5.1(t) and f5.3 from prunus avium f12/1, strains cp3.5 and cp17.2.1 from prunus avium and strain al5.1.8 from prunus cerasifera. on the basis of 16s rdna phylogeny, the strains cluster together and belong to the genus pararhizobium with type strain of pararhizobium herbae (99.6-99.8%) as their closest relative. phylogenetic analysis of the novel strains usin ...201627026286
recognition of a wide-range of s-rnases by s locus f-box like 2, a general-inhibitor candidate in the prunus-specific s-rnase-based self-incompatibility system.many species in the rosaceae, the solanaceae, and the plantaginaceae exhibit s-rnase-based gametophytic self-incompatibility (gsi). this system comprises s-ribonucleases (s-rnases) as the pistil s determinant and a single or multiple f-box proteins as the pollen s determinants. in prunus, pollen specificity is determined by a single s haplotype-specific f-box protein (sfb). the results of several studies suggested that sfb exerts cognate s-rnase cytotoxicity, and a hypothetical general inhibitor ...201627071402
the r2r3 myb transcription factor pavmyb10.1 involves in anthocyanin biosynthesis and determines fruit skin colour in sweet cherry (prunus avium l.).sweet cherry is a diploid tree species and its fruit skin has rich colours from yellow to blush to dark red. the colour is closely related to anthocyanin biosynthesis and is mainly regulated at the transcriptional level by transcription factors that regulate the expression of multiple structural genes. however, the genetic and molecular bases of how these genes ultimately determine the fruit skin colour traits remain poorly understood. here, our genetic and molecular evidences identified the r2r ...201627107393
proteomic comparison of fruit ripening between 'hedelfinger' sweet cherry (prunus avium l.) and its somaclonal variant 'hs'.the somaclonal variant hs, from sweet cherry (prunus avium l.) 'hedelfinger' (h), was previously selected for reduced tree vegetative vigor and lesser canopy density. in this work, we compared h and hs fruits at early unripe (green) and full ripe (dark red) stages by biochemical and proteomic approaches. the main biochemical parameters showed that fruit quality was not affected by somaclonal variation. the proteomic analysis identified 39 proteins differentially accumulated between h and hs frui ...201627144542
dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice.anthocyanins have been reported to have beneficial effects on obesity and obesity-related metabolic disorders (e.g., insulin resistance and dyslipidemia). the objective of this study was to examine the beneficial effects of sweet cherry anthocyanins (swcn) on high-fat diet-induced liver steatosis and investigate the underlying molecular mechanism.201727158052
complete genomic characterization of plum bark necrosis stem pitting-associated virus infecting sweet cherry in china.plum bark necrosis stem pitting-associated virus (pbnspav) causes the plum bark necrosis stem pitting-associated disease. we obtained the complete genome of a pbnspav isolate (pbnspav-ta) using small rna deep sequencing followed by overlapping rt-pcr. to our knowledge, this is the first report of a completed genome of pbnspav identified from cherry trees.201627198034
implication of abscisic acid on ripening and quality in sweet cherries: differential effects during pre- and post-harvest.sweet cherry, a non-climacteric fruit, is usually cold-stored during post-harvest to prevent over-ripening. the aim of the study was to evaluate the role of abscisic acid (aba) on fruit growth and ripening of this fruit, considering as well its putative implication in over-ripening and effects on quality. we measured the endogenous concentrations of aba during the ripening of sweet cherries (prunus avium l. var. prime giant) collected from orchard trees and in cherries exposed to 4°c and 23°c du ...027200070
genetic diversity of some iranian sweet cherry (prunus avium) cultivars using microsatellite markers and morphological traits.the aim of this study was to characterize 23 important iranian sweet cherry (prunus avium) cultivars collected from different provinces of iran and 1 foreign cultivar, which was used as control, considered for breeding programs by using 21 microsatellite markers and 27 morphological traits. in sweet cherry (prunus avium) accessions, leaf, fruit, and stone morphological characters were evaluated during two consecutive years. the study revealed a high variability in the set of evaluated sweet cher ...201627266181
preharvest salicylic acid and acetylsalicylic acid treatments preserve quality and enhance antioxidant systems during postharvest storage of sweet cherry cultivars.sweet cherries are much appreciated by consumers as a result of their organoleptic quality attributes and antioxidant properties, although they deteriorate rapidly after harvest. different preharvest strategies have been carried out to increase their quality at the time of harvest. we present data regarding the effect of preharvest salicylic acid (sa) and acetyl salicylic acid (asa) treatments on sweet cherry quality during postharvest storage.027312073
in vitro dermo-cosmetic evaluation of bark extracts from common temperate trees.wood residues produced from forestry activities represent an interesting source of biologically active, high value-added secondary metabolites. in this study, 30 extracts from 10 barks of deciduous and coniferous tree species were investigated for their potential dermo-cosmetic use. the extracts were obtained from fagus sylvatica, quercus robur, alnus glutinosa, prunus avium, acer pseudoplatanus, fraxinus excelsior, populus robusta, larix decidua, picea abies, and populus tremula after three suc ...201627352384
field evaluation of two risk indicators for predicting likelihood of pesticide transport to surface water from two orchards.two pesticide risk indicators, pesticide impact rating index (piri) and environmental potential risk indicator for pesticides (eprip), were used to determine the likelihood of off-site transport to surface water of pesticides used in a cherry (prunus avium cultivars) and an apple (malus domestica cultivars) orchard. the predictions of off-site transport of some of the pesticides were verified against actual pesticide concentrations in surface water continuously monitored over two years. to our k ...201627424118
wild prunus fruit species as a rich source of bioactive compounds.sugars, organic acids, carotenoids, tocopherols, chlorophylls, and phenolic compounds were quantified in fruit of 4 wild growing prunus species (wild cherry, bird cherry, blackthorn, and mahaleb cherry) using hplc-dad-msn. in wild prunus, the major sugars were glucose and fructose, whereas malic and citric acids dominated among organic acids. the most abundant classes of phenolic compounds in the analyzed fruit species were anthocyanins, flavonols, derivatives of cinnamic acids, and flavanols. t ...027464261
mismatch between cuticle deposition and area expansion in fruit skins allows potentially catastrophic buildup of elastic strain.the continuous deposition of cutin and wax during leaf and fruit growth is crucial to alleviate elastic strain of the cuticle, minimize the risk of failure and maintain its barrier functions. the cuticular membrane (cm) is a lipoidal biopolymer that covers primary surfaces of terrestrial plants. cms have barrier functions in water and solute transfer and pathogen invasion. these require intact cms throughout growth. this is a challenge particularly for fruit, because they increase in area from i ...027469168
linking hormonal profiles with variations in sugar and anthocyanin contents during the natural development and ripening of sweet cherries.sweet cherries are highly appreciated by consumers worldwide and are usually cold-stored during postharvest to prevent over-ripening before distribution to the market. sweet cherry is a non-climacteric fruit, for which ripening is known to be regulated by abscisic acid. here we aimed to examine the hormone profiles, including measurements of abscisic acid, auxins, cytokinins and gibberellins by ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (uhplc-ms/ms), in rela ...201627475901
ultrasound treatment: effect on physicochemical, microbial and antioxidant properties of cherry (prunus avium).the cherry was treated with ultrasonic waves (33 khz, 60 w) at different time intervals (10, 20, 30, 40, 60 min) and study was carried out to analyze the change in physico-chemical properties (tss, ph, color, acidity and firmness), antioxidant potential and microbial load of the fruit during the storage period of 15 days at 4 °c. it was observed that ultrasound treatment (us) between 30 and 40 min showed better retention of color of the fruit during the storage period. the antioxidant assays (dp ...201627478231
evaluation of physicochemical characteristics and antioxidant property of prunus avium gum exudates.in this study some physicochemical properties and elemental analysis of prunus avium gum exudates were investigated. the gum studied had, on average, 75.14% carbohydrate, 11.3% uronic acids, 1.11% protein, 7.53% moisture content (w.b.) and 3.12% ash. measured values for the angle of repose, carr's index and hausner ratio showed the good flow ability for the gum powder. the viscosity of 1% aqueous solution of the gum exhibited a newtonian type of flow and with ph reduction the swelling index was ...201627593240
validation of individual-based markov-like stochastic process model of insect behavior and a "virtual farm" concept for enhancement of site-specific ipm.the paper reports application of a markov-like stochastic process agent-based model and a "virtual farm" concept for enhancement of site-specific integrated pest management. conceptually, the model represents a "bottom-up ethological" approach and emulates behavior of the "primary ipm actors"-large cohorts of individual insects-within seasonally changing mosaics of spatiotemporally complex faming landscape, under the challenge of the local ipm actions. algorithms of the proprietary pestonfarm mo ...201627602000
characterization of community structure of culturable endophytic fungi in sweet cherry composite trees and their growth-retarding effect against pathogens.endophytic fungi have the potential to protect their host plants in stress situations. characterizing the ecology and complex interaction between these endophytes and their host plants is therefore of great practical importance, particularly in horticultural plants. among horticultural plants, fruit trees form a special category because of their longevity and because they are composites of rootstock and scion, which often belong to different plant species. here we present the first characterizat ...201627630050
potential tree species for use in urban areas in temperate and oceanic climates.this study aims to assess the potential of trees for integration in urban development by evaluating the damage caused by trees in relation to various tree characteristics. tree damage to permeable pavement systems and other urban structures such as impermeable pavements, kerbs, roads, retaining walls, footpaths, walls and buildings were assessed to identify the most suitable trees for the urban environment. one hundred square sites of 100 m × 100 m were randomly selected in greater manchester fo ...201627699277
characterization of autochthonous sweet cherry cultivars (prunus avium l.) of southern italy for fruit quality, bioactive compounds and antioxidant activity.characterizing germplasm collections of autochthonous cultivars for fruit quality traits could be a successful approach for selection, improvement of organoleptic quality and levels of antioxidants of crop produce, and development of new market opportunities and coherent strategies for conservation and valorization. the aim of the study was the evaluation of fruit physicochemical traits as well as the content of bioactive compounds and the antioxidant capacity in 25 sweet cherry autochthonous cu ...201727766652
high throughput sequencing reveals a novel fabavirus infecting sweet cherry.the genus fabavirus currently consists of five species represented by viruses that infect a wide range of hosts but none reported from temperate climate fruit trees. a virus with genomic features resembling fabaviruses (tentatively named prunus virus f, prvf) was revealed by high throughput sequencing of extracts from a sweet cherry tree (prunus avium). prvf was subsequently shown to be graft transmissible and further identified in three other non-symptomatic prunus spp. from different geographi ...201727815695
combined effect of antagonistic yeast and modified atmosphere to control penicillium expansum infection in sweet cherries cv. ambrunés.fruit decay caused by pathogenic moulds is a major concern in the postharvest quality and shelf life of fruit. blue mould decay is caused by penicillium expansum (p. expansum) and is one of the most important postharvest diseases in cherries (prunus avium l.). synthetic fungicides are the main medium used to control pathogenic moulds. however, alternative approaches are available for developing safer technologies to control postharvest disease. an integrated approach that combines biological con ...201727821356
the sweet cherry (prunus avium) flowering locus t gene is expressed during floral bud determination and can promote flowering in a winter-annual arabidopsis accession.ft gene is expressed in leaves and buds and is involved in floral meristem determination and bud development in sweet cherry. in woody fruit perennial trees, floral determination, dormancy and bloom, depends on perception of different environmental and endogenous cues which converge to a systemic signaling gene known as flowering locus t (ft). in long-day flowering plants, ft is expressed in the leaves on long days. the protein travels through the phloem to the shoot apical meristem, where it in ...201627878597
a collection of european sweet cherry phenology data for assessing climate change.professional and scientific networks built around the production of sweet cherry (prunus avium l.) led to the collection of phenology data for a wide range of cultivars grown in experimental sites characterized by highly contrasted climatic conditions. we present a dataset of flowering and maturity dates, recorded each year for one tree when available, or the average of several trees for each cultivar, over a period of 37 years (1978-2015). such a dataset is extremely valuable for characterizing ...201627922629
biochemical and physiological changes during fruit development and ripening of two sweet cherry varieties with different levels of cracking tolerance.the aim of this study was to investigate the biochemical and metabolic changes, related to oxidative stress, ethylene and respiration, cell wall modification and primary metabolism, between a high ('prime giant') and a low ('cristalina') cracking susceptible sweet cherry cultivar during growth and ripening. while cherries are referred as a non-climacteric fruit, our results show that an increase of endogenous ethylene production at earlier fruit developmental stages is parallel to colour develop ...201727951491
cell wall swelling, fracture mode, and the mechanical properties of cherry fruit skins are closely related.cell wall swelling, fracture mode (along the middle lamellae vs. across cell walls), stiffness, and pressure at fracture of the sweet cherry fruit skin are closely related. skin cracking is a common phenomenon in many crops bearing fleshy fruit. the objectives were to investigate relationships between the mode of fracture, the extent of cell wall swelling, and the mechanical properties of the fruit skin using sweet cherry (prunus avium) as a model. cracking was induced by incubating whole fruit ...201728012001
time indices of multiphasic development in genotypes of sweet cherry are similar from dormancy to cessation of pit growth.the archetypical double sigmoid-shaped growth curve of the sweet cherry drupe (prunus avium) does not address critical development from eco-dormancy to anthesis and has not been correlated to reproductive bud development. accurate representation of the growth and development of post-anthesis ovaries is confounded by anthesis timing, fruiting-density and the presence of unfertilized and defective ovaries whose growth differs from those that persist to maturation. these factors were addressed to a ...201728064193
targeted forcing improves quality, nutritional and health value of sweet cherry fruit.trade and consumers alike require premium-quality cherries with high nutritional and health values preferably of local origin. while early fruit imports cannot supply such fruit, a new technology of forcing cherry emerged for an early local supply by covering the crop in spring. in the apparent scarcity of data on the resulting fruit quality, fruit characteristics of forced cherries were compared with those without cover.201728106249
are commercial sweet cherry rootstocks adapted to climate change? short-term waterlogging and co2 effects on sweet cherry cv. 'burlat'.high co2 is able to ameliorate some negative effects due to climate change and intensify others. this study involves the sweet cherry (prunus avium) cultivar 'burlat' grafted on the 'mariana 2624', 'adara' and 'lc 52' rootstocks. in a climate chamber at two co2 concentrations, ambient (400 µmol mol-1 ) and elevated (800 µmol mol-1 ), the plants were submitted to waterlogging for 7 d, followed by 7 d of recovery after drainage. waterlogging drastically decreased the rate of photosynthesis, signif ...201728107563
pacmyba, a sweet cherry r2r3-myb transcription factor, is a positive regulator of salt stress tolerance and pathogen resistance.plant r2r3-myb transcription factors play crucial roles in stress responses. we previously isolated a r2r3-myb homolog from sweet cherry cv. hong deng, designated pacmyba (genbank accession no. kf974774). to explore the role of pacmyba in the plant stress response, we heterologously expressed pacmyba in transgenic arabidopsis thaliana plants. in a previous study, we demonstrated that pacmyba is mainly localized to the nucleus and could be induced by abscisic acid (aba). analysis of the promoter ...201728126679
non-destructive sensor-based prediction of maturity and optimum harvest date of sweet cherry fruit.(1) background: the aim of the study was to use innovative sensor technology for non-destructive determination and prediction of optimum harvest date (ohd), using sweet cherry as a model fruit, based on different ripening parameters. (2) methods: two cherry varieties in two growing systems viz. field and polytunnel in two years were employed. the fruit quality parameters such as fruit weight and size proved unsuitable to detect ohd alone due to their dependence on crop load, climatic conditions, ...028146114
economic impact of the introduction and establishment of drosophila suzukii on sweet cherry production in switzerland.first detected in switzerland in 2011, the invasive drosophila suzukii, spotted wing drosophila, has caused recurring costs for growers of berries and fruit. recommended management approaches rely on a set of methods, tailored to suit crop requirements under the prevailing local conditions. control of d. suzukii represents a substantial economic burden for growers, in terms of material, equipment, new infrastructure and extra labour. however, those growers who invest wisely to deliver unblemishe ...028208692
antifungal effect of volatile organic compounds produced by bacillus amyloliquefaciens cpa-8 against fruit pathogen decays of cherry.the present work focuses on the antifungal effect of volatile organic compounds (vocs) produced by bacillus amyloliquefaciens cpa-8 against monilinia laxa, m. fructicola and botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. vocs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. for this purpose, cpa-8 was grown on different media and cultured for 24 and 48 h at 30 °c before assays. data showed that mycelial growth inhibi ...201728213029
bioactive components of prunus avium l. black gold (red cherry) and prunus avium l. stark gold (white cherry) juices, wines and vinegars.cherries are one of the most popular fruits, characterized by attractive colour, firmness, appearance and delicious tastes. cherries are consumed fresh as well as in jams, wine, dried, candy and other processed products. cherries vary in antioxidant properties and phenolic substances. the aim of the study was to determine the effects of ethanol and acetic acid fermentation on total antioxidant activities and phenolic substances of cherry juice. total investigation of solids, ph, soluble solids, ...028242904
ascorbic acid metabolism during sweet cherry (prunus avium) fruit development.to elucidate metabolism of ascorbic acid (asa) in sweet cherry fruit (prunus avium 'hongdeng'), we quantified asa concentration, cloned sequences involved in asa metabolism and investigated their mrna expression levels, and determined the activity levels of selected enzymes during fruit development and maturation. we found that asa concentration was highest at the petal-fall period (0 days after anthesis) and decreased progressively during ripening, but with a slight increase at maturity. asa di ...201728245268
the relationship between the anthocyanin and vitamin c contents of red-fleshed sweet cherries and the ability of fruit digests to reduce hydrogen peroxide-induced oxidative stress in caco-2 cells.the present work investigated the bioprotective capacities of red-fleshed sweet cherry cultivars (prunus avium; lapins, stella, sweetheart and staccato), with distinct differences in anthocyanins and vitamin c contents, on human intestinal caco-2 cells exposed to hydrogen peroxide (h2o2)-induced oxidative stress. three assays of cell health, the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide cell viability assay, the lactate dehydrogenase membrane leakage assay and nitric oxide pro ...201728274450
spatial heterogeneity in post-dispersal predation on prunus and uvularia seeds.we investigated effects of seed density, distance from parent, and habitat (woods, open field) on post-dispersal predation risk (chiefly by rodents) for seeds of prunus virginiana (rosaceae). additional study of the habitat effect (woods, open field, treefall gap) was made with seeds of prunus avium (rosaceae) and uvularia grandiflora (liliaceae). density of prunus seeds (range 2-40 seeds/group) did not affect predation risk for individual seeds. distance from parent plants did influence predati ...198528309862
comparison of old cherry cultivars grown in czech republic by chemical composition and bioactive compounds.sweet cherry fruits are readily consumed by humans, and they contain significant quantities of bioactive compounds. fifteen sweet cherry (prunus avium l.) old cultivars grown in the czech republic were examined. in fresh fruits the contents of sugars, organic acids, and phenolic compounds, and antioxidant capacity were determined. the analyses showed that the fruits of sweet cherry are characterized by high contents of bioactive compounds. four organic acids - malic, quinic, shikimic, and fumari ...028317705
evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (prunus avium l.).identification of genetic polymorphisms and subsequent development of molecular markers is important for marker assisted breeding of superior cultivars of economically important species. sweet cherry (prunus avium l.) is an economically important non-climacteric tree fruit crop in the rosaceae family and has undergone a genetic bottleneck due to breeding, resulting in limited genetic diversity in the germplasm that is utilized for breeding new cultivars. therefore, it is critical to recognize th ...201728392892
sweet cherries from fundão possess antidiabetic potential and protect human erythrocytes against oxidative damage.cherries are one of the most appreciated summer fruits due to their attractive colour, sweet taste, high water content, low level of calories and composition in bioactive compounds which, in turn, are important to prevent some pathologies like diabetes, cardiovascular diseases and cancer. in this work we evaluated the phenolic profile and biological potential of 5 varieties of sweet cherries from fundão region (portugal) (saco, sweetheart, satin, maring and hedelfinger). a total of 23 phenolic c ...028395830
co2 effects on the waterlogging response of 'gisela 5' and 'gisela 6' (prunus cerasusxprunus canescens) sweet cherry (prunus avium) rootstocks.climate change is submitting countries of the mediterranean arc to periods of drought alternating with heavy rain and waterlogging. eventual floods along with the rising co2 in the atmosphere present an unpredictable scenario that affects crop survival. the effect of both stresses combined has been studied in sweet cherry plants. 'gisela 5' and 'gisela 6' were evaluated as rootstocks of the sweet cherry cultivar 'burlat'. plants were placed in a controlled-climate chamber for 7days, then they we ...028407490
analysis of glycosylated flavonoids extracted from sweet-cherry stems, as antibacterial agents against pathogenic escherichia coli isolates.the aim of this study was to evaluate the bioactivity of flavonoids extracted from sweet-cherry stems which are often used by a traditional system of medicine to treat gastro-intestinal and urinary tract infections but lacking any consistent scientific evidence; moreover the information about the class of phenolics, their content and the potential bioactivity of such material is very scarce. thus, in this context, we have set a research study in which we evaluated the profile and content of phen ...201728411365
microbial diversity in pitted sweet cherries (prunus avium l.) as affected by high-hydrostatic pressure treatment.sweet cherries are a highly appreciated seasonal fruit rich in anthocyanins. the purpose of the present study was to determine the effect of high-hydrostatic pressure (hhp) processing on the microbiological quality and bacterial biodiversity of sweet cherries. pitted cherries inoculated with their own epiphyte microbiota to simulate a worst-case scenario of contamination during preparation and processing were treated or not by hhp (600mpa, 8min) and stored at 4°c for 60days. hhp treatment reduce ...201628460980
flavored little cigar smoke induces cytotoxicity and apoptosis in airway epithelia.addition of flavors reduces the harsh taste of tobacco, facilitating the initiation and maintenance of addiction among youths. flavored cigarettes (except menthol) are now banned. however, the legislation on little cigars remains unclear and flavored little cigars are currently available for purchase. since inhaled tobacco smoke directly exerts toxic effects on the lungs, we tested whether non-flavored and flavored little cigar smoke exposure had the potential for harm in cultured pulmonary epit ...201728496992
the genome sequence of sweet cherry (prunus avium) for use in genomics-assisted breeding.we determined the genome sequence of sweet cherry (prunus avium) using next-generation sequencing technology. the total length of the assembled sequences was 272.4 mb, consisting of 10,148 scaffold sequences with an n50 length of 219.6 kb. the sequences covered 77.8% of the 352.9 mb sweet cherry genome, as estimated by k-mer analysis, and included >96.0% of the core eukaryotic genes. we predicted 43,349 complete and partial protein-encoding genes. a high-density consensus map with 2,382 loci was ...201728541388
dna methylation and small interference rnas participate in the regulation of mads-box genes involved in dormancy in sweet cherry (prunus avium l.).epigenetic modifications can yield information about connections between genotype, phenotype variation and environmental conditions. bud dormancy release in temperate perennial fruit trees depends on internal and environmental signals such as cold accumulation and photoperiod. previous investigations have noted the participation of epigenetic mechanisms in the control of this physiological process. we examined whether epigenetic modifications were modulated in mads-box genes, potential candidate ...201728541567
metabolomic and physico-chemical approach unravel dynamic regulation of calcium in sweet cherry fruit physiology.calcium (ca(2)) nutrition has a significant role in fruit physiology; however, the underlying mechanism is still unclear. in this study, fruit quality in response to cacl2, applied via foliar sprays (ca(2)) or/and hydro-cooling water (cahc), was characterized in 'lapins' cherries at harvest, just after cold storage (20 days at 0 °c) as well as after cold storage followed by 2 days at 20 °c, herein defined as shelf-life period. data indicated that pre- and post-harvest ca(2+) applications increas ...028551418
two minuses can make a plus: waterlogging and elevated co2 interactions in sweet cherry (prunus avium) cultivars.the increase in the ambient concentration of co2 and other greenhouse gases is producing climate events that can compromise crop survival. however, high co2 concentrations are sometimes able to mitigate certain stresses such as salinity or drought. in this experiment, the effects of waterlogging and co2 are studied in combination to elucidate the eventual response in sweet cherry trees. for this purpose, four sweet cherry cultivars ('burlat', 'cashmere', 'lapins, and 'new star') were grafted on ...028568609
phenolic compounds profile and antioxidant properties of six sweet cherry (prunus avium) cultivars.sweet cherry (prunus avium) fruits are a nutritionally important food rich in dietary phenolic compounds. the aim of this study was to investigate the phenolic profile and chemometric discrimination of fruits from six cherry cultivars using a quantitative metabolomics approach, which combine non-targeted mass spectrometry and chemometric analysis. the assessment of the phenolic fingerprint of cherries allowed the tentative identification of 86 compounds. a total of 40 chlorogenic acids were iden ...028578036
cyanogenic glucosides and derivatives in almond and sweet cherry flower buds from dormancy to flowering.almond and sweet cherry are two economically important species of the prunus genus. they both produce the cyanogenic glucosides prunasin and amygdalin. as part of a two-component defense system, prunasin and amygdalin release toxic hydrogen cyanide upon cell disruption. in this study, we investigated the potential role within prunasin and amygdalin and some of its derivatives in endodormancy release of these two prunus species. the content of prunasin and of endogenous prunasin turnover products ...201728579996
physical rupture of the xylem in developing sweet cherry fruit causes progressive decline in xylem sap inflow rate.xylem flow is progressively shut down during maturation beginning with minor veins at the stylar end and progressing to major veins and finally to bundles at the stem end. this study investigates the functionality of the xylem vascular system in developing sweet cherry fruit (prunus avium l.). the tracers acid fuchsin and gadoteric acid were fed to the pedicel of detached fruit. the tracer distribution was studied using light microscopy and magnetic resonance imaging. the vasculature of the swee ...201728623562
therapeutic effects of aqueous extracts of cerasus avium stem on ethylene glycol- induced kidney calculi in rats.to investigate the therapeutic effects of the aqueous extract of cerasus avium stem on kidney calculi.201728670670
multi-approach metabolomics analysis and artificial simplified phytocomplexes reveal cultivar-dependent synergy between polyphenols and ascorbic acid in fruits of the sweet cherry (prunus avium l.).fruits of the sweet cherry (prunus avium l.) accumulate a range of antioxidants that can help to prevent cardiovascular disease, inflammation and cancer. we tested the in vitro antioxidant activity of 18 sweet cherry cultivars collected from 12 farms in the protected geographical indication region of marostica (vicenza, italy) during two growing seasons. multiple targeted and untargeted metabolomics approaches (nmr, lc-ms, hplc-dad, hplc-uv) as well as artificial simplified phytocomplexes repres ...201728732012
optoacoustic determination of analytical parameters and physicochemical constants in highly concentrated solutions of chromophores.determination of chromophores of various classes-rosaniline (fuchsine), cyanidin-3-o-glucoside, tris(1,10-phenanthroline) iron(ii), and phenol red - in their concentrated solutions near their solubility limits is performed with the optoacoustic technique for optically dense solutions; light-absorption coefficients of samples range from 0.5 to 500cm-1. the assessment of these substances in organo-aqueous and organic solvents is possible up to ca. 0.1moll-1. characteristic stability and rate const ...201728738569
polyphenol patterns to trace sweet (prunus avium) and tart (prunus cerasus) varieties in cherry jam.the aim of this work was to assess whether the characteristic polyphenol traits of cherry biotypes persisted in thermally processed cherry products, such as jam. thus, the rp-hplc-diode array detector profiles of both colorless polyphenols and anthocyanins from three cherry varieties (two sweet and one tart cherry) were compared with those of low-sugar jam sourced from the same cultivars. individual components were characterized by mass spectrometry. the total phenolic and total anthocyanin cont ...201728740288
changes in carbohydrate levels and relative water content (rwc) to distinguish dormancy phases in sweet cherry.perennial trees require chilling, i.e. a period of cold temperature in the winter, for flowering next spring; sweet cherry is particularly prone to lack of chilling. the objective of this study is to identify possible transition points to clearly distinguish dormancy phases by relating carbohydrate and relative water content (rwc) in reproductive buds to concomitant chilling fulfilment. this contribution proposes the use of four transition points between the dormancy phases and their characteriz ...201728759789
transcriptome and metabolite changes during hydrogen cyanamide-induced floral bud break in sweet cherry.release of bud dormancy in perennial woody plants is a temperature-dependent process and thus flowering in these species is heavily affected by climate change. the lack of cold winters in temperate growing regions often results in reduced flowering and low fruit yields. this is likely to decrease the availability of fruits and nuts of the prunus spp. in the near future. in order to maintain high yields, it is crucial to gain detailed knowledge on the molecular mechanisms controlling the release ...201728769948
physiological and de novo transcriptome analysis of the fermentation mechanism of cerasus sachalinensis roots in response to short-term waterlogging.cerasus sachalinensis is widely used in cool regions as a sweet cherry rootstock and is known for its sensitivity to soil waterlogging and waterlogging stress. however, the limited availability of cerasus genomic resources has considerably restricted the exploration of its waterlogging response mechanism. to understand its reaction to short-term waterlogging, we analyzed the physiology and transcriptomes of c. sachalinensis roots in response to different waterlogging durations.201728830345
the evaluation of extraction techniques for tetranychus urticae (acari: tetranychidae) from apple (malus domestica) and cherry (prunus avium) leaves.tetranychus urticae is a widespread polyphagous mite, found on a variety of fruit crops. tetranychus urticae feeds on the underside of the leaves perforating plant cells and sucking the cell contents. foliar damage and excess webbing produced by t. urticae can reduce fruit yield. assessments of t. urticae populations while small provide reliable and accurate ways of targeting control strategies and recording their efficacy against t. urticae. the aim of this study was to evaluate four methods fo ...201728831615
patterns of low temperature induced accumulation of dehydrins in rosaceae crops-evidence for post-translational modification in apple.important crop plants of rosaceae family are often damaged during winter due to the lack of acclimation and cold hardiness. one of the cellular responses of plants to cold stress is the accumulation of dehydrin proteins. we studied the expression of dehydrins in several rosaceae species during low temperature treatment in vitro. microshoots of pyrus communis, malus×domestica, fragaria vesca, fragaria×ananassa, prunus cerasus and prunus avium cultivars were grown in low temperature conditions. ge ...201728886453
cloning and expression profiling of the pacsnrk2 and pacpp2c gene families during fruit development, aba treatment, and dehydration stress in sweet cherry.plant snf1-related protein kinase 2 (snrk2) and protein phosphatase 2c (pp2c) family members are core components of the aba signal transduction pathway. snrk2 and pp2c proteins have been suggested to play crucial roles in fruit ripening and improving plant tolerance to drought stress, but supporting genetic information has been lacking in sweet cherry (prunus avium l.). here, we cloned six full-length snrk2 genes and three full-length pp2c genes from sweet cherry cv. hong deng. quantitative pcr ...201728926798
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