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using survival analysis to investigate the effect of uv-c and heat treatment on storage rot of strawberry and sweet cherry.ultraviolet light and heat treatment are proposed as alternative techniques for the use of chemicals to reduce the development of the spoilage fungi botrytis cinerea and monilinia fructigena on strawberry and sweet cherry, respectively, during storage. in order to investigate the effect of both physical techniques on microbial inactivation and on fruit quality, inoculated berries were subjected to different temperatures (40-48 degrees c) and uv-c doses (0.05-1.50 j/cm2). for each condition, 20 b ...200211934026
development of microsatellite markers in peach [ prunus persica (l.) batsch] and their use in genetic diversity analysis in peach and sweet cherry ( prunus avium l.).we report the sequence of 41 primer pairs of microsatellites from a ct-enriched genomic library of the peach cultivar 'merrill o'henry'. ten microsatellite-containing clones had sequences similar to plant coding sequences in databases and could be used as markers for known functions. for microsatellites segregating at least in one of the two prunus f(2) progenies analyzed, it was possible to demonstrate mendelian inheritance. microsatellite polymorphism was evaluated in 27 peach and 21 sweet che ...200212582570
effect of cold treatment on survival and development of codling moth (lepidoptera: tortricidae) in cherry.'bing' cherries, prunus avium l., were obtained from an organic orchard and a conventional commercial orchard. the two groups were examined separately in replicated tests infested with each fruit initially infested with a first-instar codling moth, cydia pomonella (l.). to simulate commercial postharvest holding conditions, the treatments were 0 (control), 1, 2, 4, 7, 10, and 14 d cold storage at 3.3 degrees c. the fruits were examined three or more times to determine larval survival, life stage ...200211942758
studies on water transport through the sweet cherry fruit surface: iii. conductance of the cuticle in relation to fruit size.rain-cracking of sweet cherry fruit has been related to water absorption through the fruit surface and large fruit has been reported to be more susceptible to cracking than small fruit. therefore, the effect of fruit size on water conductance of the cuticular membrane (cm) of exocarp segments excised from cheek, suture or stylar end region of mature sweet cherry fruit (prunus avium l. cv. sam) was investigated. segments consisting of epidermis, hypodermis and several layers of mesocarp cells wer ...200212060264
evolutionary analysis of s-rnase genes from rosaceae species.eight new cdna sequences for s-rnases were cloned and analysed from almond (prunus dulcis) cultivars of european origin, and compared to published sequences from other rosaceae species. insertions/deletions of 10-20 amino acid residues were detected in the rc4 and c5 domains of s-rnases from almond and sweet cherry. the s-rnases of the prunus species and those of the genera malus and pyrus formed two distinct groups on phylogenetic analysis. nucleotide substitutions were analysed in the s-rnase ...200211919717
studies on water transport through the sweet cherry fruit surface. 7. fe3+ and al3+ reduce conductance for water uptake.the effects of the chloride salts licl, cacl(2), mgcl(2), alcl(3), eucl(3), and fecl(3) and the iron salts fecl(2), fecl(3), fe(no(3))(3), feso(4), and fe(2)(so(4))(3) on water conductance of exocarp segments (es) and rates of water uptake into detached sweet cherry fruit (prunus avium l. cv. adriana, early rivers, namare, namosa, and sam) were studied. es were excised from the cheek of mature fruit and mounted in stainless steel diffusion cell; water penetration was monitored gravimetrically fr ...200212475277
molecular characterisation of sweet cherry (prunus avium l.) genotypes using peach [prunus persica (l.) batsch] ssr sequences.a total of 76 sweet cherry genotypes were screened with 34 microsatellite primer pairs previously developed in peach. amplification of ssr loci was obtained for 24 of the microsatellite primer pairs, and 14 of them produced polymorphic amplification patterns. on the basis of polymorphism and quality of amplification, a set of nine primer pairs and the resulting 27 informative alleles were used to identify 72 genotype profiles. of these, 68 correspond to unique cultivar genotypes, and the remaini ...200212080370
radiocaesium contamination of meadow vegetation--time-dependent variability and influence of soil characteristics at grassland sites in austria.long-term trends of 137cs and 40k concentrations in meadow grass and soil-plant transfer data at eight different sites in upper austria are presented. geometric means of 137cs tf-data and tagg values vary between 0.03-1.06 and 0.0005-0.0184 depending on site, respectively. 40k results are less variable with tf values covering a range of 0.31-2.01. only at one site was a significant decrease of 137cs concentration (decay-corrected) in meadow vegetation observed during the observation period 1992- ...200211814163
a cdna encoding a cold-induced glycine-rich rna binding protein from prunus avium expressed in embryonic axes.a cdna clone encoding a presumed full-length glycine-rich ribonucleic acid (rna) binding protein was isolated from a lambda-zap express cdna library generated from primarily nondormant prunus avium (wild cherry) embryonic axes. the cdna, designated pa-rrm-grp1 (prunus avium rna recognition motif glycine-rich protein 1), contains a single n-terminal rna recognition motif (rrm) and single c-terminal glycine-rich domain. the glycine-rich domain is unusually long at 91 amino acids, 58 of which are g ...200314597401
allele-specific pcr detection of sweet cherry self-incompatibility (s) alleles s1 to s16 using consensus and allele-specific primers.pcr-based identification of all 13 known self-incompatibility (s) alleles of sweet cherry is reported. two pairs of consensus primers were designed from our previously published cdna sequences of s(1) to s(6) s-rnases, the stylar components of self-incompatibility, to reveal length variation of the first and the second introns. with the exception of the first intron of s(13), these also amplified s(7) to s(14) and an allele previously referred to as s(x), which we now label s(16). the genomic pc ...200314523529
direct observations of the redox states of frozen cherry buds by a unique in vivo esr.low temperatures cause cellular damage in flower buds of the sweet cherry (prunus avium l. cv. satohnishiki). in this study, the redox states within the cherry buds suffering freezing damage were non-destructively observed by a unique in vivo electron spin resonance (esr) technique with a spin probe such as carbamoyl-proxyl. the esr signals of carbamoyl-proxyl-treated bud were continuously recorded under freezing and thawing condition, which was decreased to approximately -4 degrees c and mainta ...200314511650
recycling of nitrogen in the xylem of prunus avium trees starts when spring remobilization of internal reserves declines.nitrogen (n) storage capacity of cherry (prunus avium l.) trees grown in sand culture was preconditioned by applying contrasting n supplies for one year. during the spring of the following year, a constant amount of 15n was supplied and the dynamics of n remobilization and root uptake were characterized as a function of internal n status of the trees. to calculate the flux of n through xylem, both xylem sap n concentration and whole-tree transpiration rates were measured. by comparing the cumula ...200312975130
identification of a non-s rnase, a possible ancestral form of s-rnases, in prunus.this study identifies and characterizes a basic non-s rnase in the styles with stigmas of sweet cherry (prunus avium l.), a member of the rosaceae subfamily amygdaloideae, which has an rnase-based gametophytic self-incompatibility system. internal sequences of putative non-s rnases (rnase pa1 and pa2) were determined, and a cdna for pa1 was obtained. the deduced amino acid sequence of pa1 contained two conserved sequence motifs essential for t2/ s-type rnase activity. pa1 shows 20-30% sequence i ...200312715157
characterization of microsatellites in wild and sweet cherry (prunus avium l.)--markers for individual identification and reproductive processes.nuclear microsatellites were characterized in prunus avium and validated as markers for individual and cultivar identification, as well as for studies of pollen- and seed-mediated gene flow. we used 20 primer pairs from a simple sequence repeat (ssr) library of prunus persica and identified 7 loci harboring polymorphic microsatellite sequences in p. avium. in a natural population of 75 wild cherry trees, the number of alleles per locus ranged from 4 to 9 and expected heterozygosity from 0.39 to ...200312669801
a pollen-expressed gene for a novel protein with an f-box motif that is very tightly linked to a gene for s-rnase in two species of cherry, prunus cerasus and p. avium.this study describes a novel f-box protein gene in the s-locus of sour cherry (prunus cerasus) and sweet cherry (p. avium). the gene showed an s-haplotype-specific sequence polymorphism and the expression was specific to pollen. genomic dna blot analysis of eight sweet cherry cultivars with the probe for the f-box protein gene under low stringency conditions yielded rflp bands specific to the s-haplotypes of each cultivar. we discuss the possibility of the gene for the f-box protein being a cand ...200312881505
characterization of the s-rnase promoters from sweet cherry (prunus avium l.).genomic dna fragments containing the s(3)-, s(4)-, and s(6)-rnase genes were isolated from the sweet cherry (prunus avium l.) and sequenced. comparison of the 5'-flanking sequences of these three s-rnases indicated that a highly conserved region (designated cr) existed just upstream from the putative tata boxes. we postulate that cr contains cis-regulatory element(s) involved in pistil expression. to examine the activity of the isolated s-rnase promoters of sweet cherry in the pistil, we transie ...200312773819
correlating ige reactivity with three-dimensional structure.this commentary discusses the work of neudecker et al. in this issue of the biochemical journal in which site-directed mutagenesis and nmr spectroscopy have been used to analyse in detail the ige-binding capacity of two cross-reactive allergens: apg1.0101 from celery ( apium graveolens ) and pru av 1 from cherry ( prunus avium ), which are both members of the pathogenesis-related allergen family. the study, showing that the ige-binding epitopes are highly patient specific, will have a profound i ...200314602045
mutational epitope analysis of pru av 1 and api g 1, the major allergens of cherry (prunus avium) and celery (apium graveolens): correlating ige reactivity with three-dimensional structure.birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific ige cross-reacting with homologous food allergens. the tertiary structure of pru av 1, the major cherry (prunus avium) allergen, for example, is nearly identical with bet v 1, the major birch (betula verrucosa) pollen allergen. in order to define cross-reactive ige epitopes, we generated and analysed mutants of pru av 1 and api g 1.0101, the major celery (apium graveolens) allergen, by immunoblotti ...200312943529
ozone air pollution and foliar injury development on native plants of switzerland.the objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. the study was conducted from the beginning of may through the end of august during 2000 and 2001 using an open-top chamber research facility located within the lattecaldo cantonal forest nursery in canton ticino, southern switzerland (600 m asl) ...200312804826
[identifying the s genotypes of sweet cherry (prunus avium l.) cultivars].this report identified s-rnase genes (s genes) of sweet cherry (prunus avium l.), presented the sequences of s genes by using a pair of specific primers pruc2 and pruc4r based on the conserved regions c2 and rc4 of rosaceous s-rnase genes and researched the s gene specific products from the genomic dnas of different cultivars in which most of the s genotypese were unknown. the bands of pcr were cloned and their sequences were compared in genbank. four s genes were defined and the conclusion was ...200415552051
characterization of pseudomonas pathovars isolated from rosaceous fruit trees in east algeria.a survey of bacterial diseases due to pseudomonas on rosaceous fruit trees was conducted. in forty two orchards located in the constantine region ( east algeria). pseudomonas isolates were identified on the bases of their cultural and biochemical characteristics . a total of fifty nine phytopathogenic bacteria were isolated from diseased pome and stone fruit trees. thirty one strains comparable to pseudomonas syringae pv. syringae were isolated from cherry (prunus avium l.), plum (p. domestica l ...200415756824
flavanol binding of nuclei from tree species.light microscopy was used to examine the nuclei of five tree species with respect to the presence of flavanols. flavanols develop a blue colouration in the presence of a special p-dimethylaminocinnamaldehyde (dmaca) reagent that enables those nuclei loaded with flavanols to be recognized. staining of the nuclei was most pronounced in both tsuga canadensis and taxus baccata, variable in metasequoia glyptostroboides, faint in coffea arabica and minimal in prunus avium. hplc analysis showed that th ...200414595514
changes in strain and deposition of cuticle in developing sweet cherry fruit.changes in surface area, deposition and elastic strain of the cuticular membrane (cm) were monitored during development of sweet cherry (prunus avium l.) fruit. fruit mass and surface area ('sam') increased in a sigmoidal pattern between 16 and 85 days after full bloom (dafb) with maximum rates of 0.35 g day(-1) and 0.62 cm(2) day(-1), respectively. rates of total area strain, namely the sum of elastic plus plastic strain, were highest in cheek and stem cavity regions followed by stylar and sutu ...200415032829
effect of ripeness and postharvest storage on the phenolic profiles of cherries (prunus avium l.).the phenolic compounds hydroxycinnamates, anthocyanins, flavonols, and flavan-3-ols of sweet cherry cultivars burlat, saco, summit, and van harvested in 2001 and 2002 were quantified by hplc-dad. phenolics were analyzed at partially ripe and ripe stages and during storage at 15 +/- 5 degrees c (room temperature) and 1-2 degrees c (cool temperature). neochlorogenic and p-coumaroylquinic acids were the main hydroxycinnamic acid derivatives, but chlorogenic acid was also identified in all cultivars ...200414759143
identification of incompatibility alleles in the tetraploid species sour cherry.the incompatibility genetics of sour cherry ( prunus cerasus), an allotetraploid species thought to be derived from sweet cherry (diploid) and ground cherry (tetraploid), were investigated by test crossing and by analysis of stylar ribonucleases which are known to be the products of incompatibility alleles in sweet cherry. stylar extracts of 36 accessions of sour cherry were separated electrophoretically and stained for ribonuclease activity. the zymograms of most accessions showed three bands, ...200414689184
two-dimensional tension tests in plant biomechanics--sweet cherry fruit skin as a model system.splitting of fruits is a function of two-dimensional tension caused by different growth rates of tissues and turgor, especially water uptake shortly before harvest. in order to analyse the mechanical properties of spheroid plant material close to stress-strain conditions in vivo, a new hydraulic two-dimensional testing device was set up. sweet cherry (prunus avium l.) fruit skin was chosen as a model system. the recorded pressure-deflection curves were non-linear, with a considerable initial "la ...200415248126
hyperhydricity of prunus avium shoots cultured on gelrite: a controlled stress response.hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. hyperhydric shoots are characterised by a translucent aspect due to a chlorophyll deficiency, a not very developed cell wall and a high water content. hyperhydricity of prunus avium shoots was expressed in vitro in one multiplication cycle by replacing the gelling agent agar (normal shoots: ns) by gelrite (hyperhydric shoots: hs). p. avium shoots evolving towards the hyperhydric state produce ...200415246065
cloning and characterization of genomic dna sequences of four self-incompatibility alleles in sweet cherry ( prunus avium l.).gametophytic self-incompatibility (gsi) in sweet cherry is determined by a locus s with multiple alleles. in the style, the s-locus codifies for an allele-specific ribonuclease ( s-rnase) that is involved in the rejection of pollen that carries the same s allele. in this work we report the cloning and genomic dna sequence analysis including the 5' flanking regions of four s-rnases of sweet cherry ( prunus avium l., rosaceae). dna from the cultivars ferrovia, pico colorado, taleguera brillante an ...200412955210
maillard reaction and enzymatic browning affect the allergenicity of pru av 1, the major allergen from cherry (prunus avium).the influence of thermal processing and nonenymatic as well as polyphenoloxidase-catalyzed browning reaction on the allergenicity of the major cherry allergen pru av 1 was investigated. after thermal treatment of the recombinant protein rpru av 1 in the absence or presence of carbohydrates, sds-page, enzyme allergosorbent tests, and inhibition assays revealed that thermal treatment of rpru av 1 alone did not show any influence on the ige-binding activity of the protein at least for 30 min, thus ...200415186129
effect of temperature on pollen tube kinetics and dynamics in sweet cherry, prunus avium (rosaceae).prevailing ambient temperature during the reproductive phase is one of several important factors for seed and fruit set in different plant species, and its consequences on reproductive success may increase with global warming. the effect of temperature on pollen performance was evaluated in sweet cherry (prunus avium l.), comparing as pollen donors two cultivars that differ in their adaptation to temperature. 'sunburst' is a cultivar that originated in canada with a pedigree of cultivars from no ...200421653411
expression of a sweet cherry dreb1/cbf ortholog in arabidopsis confers salt and freezing tolerance.dehydration responsive element binding protein 1 (dreb1)/c-repeat binding factor (cbf) induces the expression of many stress-inducible genes in arabidopsis. we have previously reported the identification of three dreb1/icbf homologs from sweet cherry (prunus avium). to identify the function of these homologs, one of the genes, cig-b, was transformed into arabidopsis. in one of the transgenic plant lines, the dreb1/cbf target gene cor15a was induced in the absence of stress treatment. the cor15a- ...200415535126
genetic relationships between diploid and allotetraploid cherry species (prunus avium, prunus x gondouinii and prunus cerasus).prunus avium l. (diploid, aa, 2n=2x=16), prunus cerasus l. (allotetraploid, aaff, 2n=4x=32) species, and their hybrid prunus x gondouinii rehd., constitute the most widely cultivated cherry tree species. p. cerasus is supposed to be an hybrid species produced by the union of unreduced p. avium gametes and normal p. fruticosa gametes. a continuum of morphological traits between these three species makes their assignation difficult. the aim of this paper is to study the genetic relationships betwe ...200415354194
the role of polar auxin transport through pedicels of prunus avium l. in relation to fruit development and retention.it was investigated whether premature fruit abscission in prunus avium l. was triggered by a reduction in polar auxin transport (pat). the capacity of pedicels to transport tritiated iaa ([3h]-iaa) via the pat pathway was measured at intervals throughout flower and fruit development. the extent of passive diffusion, assessed by concurrent applications of [14c]-benzoic acid ([14c]-ba), was negligible. transported radioactivity recovered from agar blocks eluted at the same retention time as authen ...200415310825
the s haplotype-specific f-box protein gene, sfb, is defective in self-compatible haplotypes of prunus avium and p. mume.many prunus species, including sweet cherry and japanese apricot, of the rosaceae, display an s-rnase-based gametophytic self-incompatibility (gsi). the specificity of this outcrossing mechanism is determined by a minimum of two genes that are located in a multigene complex, termed the s locus, which controls the pistil and pollen specificities. sfb, a gene located in the s locus region, encodes an f-box protein that has appropriate s haplotype-specific variation to be the pollen determinant in ...200415272875
loss of pollen-s function in two self-compatible selections of prunus avium is associated with deletion/mutation of an s haplotype-specific f-box gene.recently, an s haplotype-specific f-box (sfb) gene has been proposed as a candidate for the pollen-s specificity gene of rnase-mediated gametophytic self-incompatibility in prunus (rosaceae). we have examined two pollen-part mutant haplotypes of sweet cherry (prunus avium). both were found to retain the s-rnase, which determines stylar specificity, but one (s3' in ji 2434) has a deletion including the haplotype-specific sfb gene, and the other (s4' in ji 2420) has a frame-shift mutation of the h ...200515598801
combined heat and controlled atmosphere quarantine treatments for control of codling moth in sweet cherries.nonchemical quarantine treatments, using a combination of short-duration high temperatures under low oxygen, elevated carbon dioxide atmospheric environment were developed to control codling moth in sweet cherries, prunus avium (l.). the two treatments developed are a chamber temperature of 45 degrees c for 45 min and a chamber temperature of 47 degreesc for 25 min under a 1% oxygen, 15% carbon dioxide, -2 degrees c dew point environment. both these treatments have been shown to provide control ...200516022297
chemical constituents and antioxidant activity of sweet cherry at different ripening stages.the development and ripening process of sweet cherry (prunus avium l. cv. 4-70) on the tree was evaluated. for this purpose, 14 different stages were selected in accordance with homogeneous size and color. some parameters related to fruit quality, such as color, texture, sugars, organic acids, total antioxidant activity, total phenolic compounds, anthocyanins, and ascorbic acid were analyzed. the results revealed that in sweet cherry, the changes in skin color, glucose and fructose accumulation, ...200515796619
determining self-incompatibility genotypes in belgian wild cherries.abstract the self-incompatibility (s) genotypes of a collection of 65 belgian accessions of wild cherry, selected within two populations and planted in a seed orchard, were determined using polymerase chain reaction (pcr) methods. initially, dna extracts were amplified with consensus primers that amplify across the second intron of the s-ribonuclease gene which shows considerable length polymorphism. the provisional genotypes deduced were checked with the appropriate allele-specific primers for ...200515773927
influence of genotype-temperature interaction on pollen performance.pollen competition and selection have significant evolutionary consequences, but very little is known about how they can be modulated. we have examined in cherry (prunus avium l.) how pollen performance is affected by the genotype of the pollen and by the environmental conditions under which it grows, namely the pistilar tissue and temperature. the different pollen donor genotypes tested in this work differed in their behaviour both in vitro and in vivo and this behaviour was modulated depending ...200516313462
studies on water transport through the sweet cherry fruit surface: ix. comparing permeability in water uptake and transpiration.water uptake and transpiration were studied through the surface of intact sweet cherry (prunus avium l.) fruit, exocarp segments (es) and cuticular membranes (cm) excised from the cheek of sweet cherry fruit and astomatous cm isolated from schefflera arboricola (hayata) hayata, citrus aurantium l., and stephanotis floribunda brongn. leaves or from lycopersicon esculentum mill. and capsicum annuum l. var. annuum fasciculatum group fruit. es and cm were mounted in diffusion cells. water (deionized ...200515338307
molecular basis of pollen-related food allergy: identification of a second cross-reactive ige epitope on pru av 1, the major cherry (prunus avium) allergen.birch (betula verrucosa) pollen-associated food allergy is a well-characterized syndrome, which is due to the cross-reactivity of ige antibodies to homologous allergens in various foods. one crossreacting area on the major birch pollen allergen bet v 1 and its homologue in cherry (prunus avium) pru av 1 has already been identified. this is the so-called 'p-loop' region, which encompasses amino acid residues around position 45 and is found on the two virtually identical tertiary protein structure ...200515330760
in vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (prunus avium l.).regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. "schneiders", "sweetheart", "starking hardy giant", "kordia" and "regina" (prunus avium l.). the influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. both, dkw/ ...200515948004
influence of selected fruit tree pollen on life history of euseius stipulatus (acari: phytoseiidae).euseius stipulatus (athias-henriot) is a predatory mite widespread in the mediterranean region considered to be important for the biological control of spider mites in citrus orchards. development, survival and reproduction of this phytoseiid mite feeding on seven commercially obtained pollen were studied under constant laboratory conditions (20 +/- 1 degrees c, rh 65 +/- 5%, photoperiod 16l: 8d h). mites were kept individually at rearing units with ample quantity of almond (prunus amygdalus bat ...200516082919
enhancement of biocontrol activity of cryptococcus laurentii by silicon and the possible mechanisms involved.abstract exogenous application of silicon (si) in the form of sodium metasilicate reduced disease development caused by penicillium expansum and monilinia fructicola in sweet cherry fruit at 20 degrees c. the inhibition of fruit decay was correlated closely with si concentrations. silicon at concentrations of 1%, in combination with the biocontrol agent cryptococcus laurentii at 1 x 10(7) cells per ml, provided synergistic effects against both diseases. population dynamics of c. laurentii were s ...200518943838
reduced-risk management of rhagoletis cerasi flies (host race prunus) in combination with a preliminary phenological model.seasonal flight activity of rhagoletis cerasi (l.) (diptera: tephritidae) adults was monitored using yellow sticky traps at sweet cherry orchards under different management regimes in bursa, northwestern turkey, during 1997-1998. in the reduced-risk backyard orchards, soil ploughing in the fall or spring to destroy the pupae was combined with a single application of an insecticide, while conventionally managed orchards received six to seven insecticide applications for controlling adults. traps ...200620298111
bee population returns and cherry yields in an orchard pollinated with osmia lignaria (hymenoptera: megachilidae).during 1998-2003, we used populations of the solitary bee osmia lignaria say to pollinate a commercial sweet cherry orchard in northern utah. bee densities released each year ranged from 1290 to 1857 females/ha, with approximately twice as many males. female progeny produced each year were greater than parental populations released, except in 2003, when nesting was poor due to bird predation. despite poor weather during bloom, and in contrast to most other local producers, the study orchard prod ...200616686139
molecular characterization of three non-functional s-haplotypes in sour cherry (prunus cerasus).tetraploid sour cherry (prunus cerasus) exhibits a genotype-dependent loss of gametophytic self-incompatibility that is caused by the accumulation of non-functional s-haplotypes with disrupted pistil component (stylar-s) and/or pollen component (pollen-s) function. genetic studies using diverse sour cherry germplasm identified non-functional s-haplotypes for which an equivalent wild-type s-haplotype was present in sweet cherry (prunus avium), a diploid progenitor of sour cherry. in all cases, th ...200616915517
comparative analysis of the within-population genetic structure in wild cherry (prunus avium l.) at the self-incompatibility locus and nuclear microsatellites.gametophytic self-incompatibility (si) systems in plants exhibit high polymorphism at the si controlling s-locus because individuals with rare alleles have a higher probability to successfully pollinate other plants than individuals with more frequent alleles. this process, referred to as frequency-dependent selection, is expected to shape number, frequency distribution, and spatial distribution of self-incompatibility alleles in natural populations. we investigated the genetic diversity and the ...200616968267
natural and recombinant molecules of the cherry allergen pru av 2 show diverse structural and b cell characteristics but similar t cell reactivity.cherry allergy is often reported in the context of allergy to other fruits of the rosaceae family and pollinosis to trees because of cross-reactive allergens. allergic reactions to cherry are reported by 19-29% of birch pollen-allergic patients. pru av 2, identified as a thaumatin-like protein (tlp) from sweet cherry, was recognized by the majority of cherry-allergic patients in immunoblotting.200616499648
combination of antagonistic yeasts with two food additives for control of brown rot caused by monilinia fructicola on sweet cherry fruit.to evaluate beneficial effect of two food additives, ammonium molybdate (nh4-mo) and sodium bicarbonate (nabi), on antagonistic yeasts for control of brown rot caused by monilinia fructicola in sweet cherry fruit under various storage conditions. the mechanisms of action by which food additives enhance the efficacy of antagonistic yeasts were also evaluated.200616478490
comparative analysis of plant finds from early roman graves in ilok (cuccium) and sćitarjevo (andautonia), croatia--a contribution to understanding burial rites in southern pannonia.a comparative archaeobotanical analysis of the plant remains from the early roman incineration graves in ilok and sćitarjevo shows the existence of a complex burial ritual, but at the same time enables a better understanding of the agriculture and trade of the 1st/early 2nd century ad in southern pannonia. most of the cereals found (hordeum vulgare, panicum miliaceum, triticum monococcum, t. dicoccon, t. aestivum i t. cf. spelta), the legumes (lens culinaris, vicia ervilia) and the fruit contrib ...200616848163
combined heat and controlled atmosphere quarantine treatments for control of western cherry fruit fly in sweet cherries.nonchemical quarantine treatments, using a combination of short duration high temperatures under low oxygen, elevated carbon dioxide atmospheric environment were developed to control western cherry fruit fly, rhagoletis indifferens curran, in sweet cherries, prunus avium (l.). the two treatments developed use a chamber temperature of 45 degrees c for 45 min and a chamber temperature of 47 degreesd c for 25 min, both under a 1% oxygen, 15% carbon dioxide, -2 degrees c dew point environment. both ...200616813295
isolation of s-locus f-box alleles in prunus avium and their application in a novel method to determine self-incompatibility genotype.this study characterises a series of 12 s-locus haplotype-specific f-box protein genes (sfb) in cherry (prunus avium) that are likely candidates for the pollen component of gametophytic self-incompatibility in this species. primers were designed to amplify 12 sfb alleles,including the introns present in the 50 untranslated region;sequences representing the s-alleles s1, s2, s3, s4, s40, s5,s6, s7, s10, s12, s13 and s16 were cloned and characterized. [the nucleotide sequences reported in this pap ...200616365757
detecting local establishment strategies of wild cherry (prunus avium l.).p. avium, a pioneer tree species that colonizes early forest successional stages, is assumed to require an effective strategy allowing stably repeatable rounds of local establishment, dispersal and local extinction. consequently, the early replacement of cherry by climax tree species makes the establishment of several local generations very unlikely, especially in central european continuous cover forests. this has to be seen in connection with the mixed reproduction system involving asexual rep ...200617020607
heterozygote excess in a self-incompatible and partially clonal forest tree species -- prunus avium l.wild cherry (prunus avium l.), a partially asexual self-incompatible forest tree, shows heterozygote excess, which is a poorly studied phenomenon. in three natural populations, we found significant heterozygote excess at almost all investigated loci (eight microsatellites and markers for the self-incompatibility locus). we examined four hypotheses to account for this observed heterozygote excess. first, negative f(is) can result from a lack of selfed progeny in small populations of outcrossing s ...200616780428
cracking susceptibility of sweet cherries (prunus avium l.) under different conditions.rain and hail during ripening and harvesting season can cause yield losses up to 90 % in sweet cherry cultivations in germany. particularly, high yield losses after precipitation are due to the cracking and the following rotting process through bacteria and fungi's. in order to protect the crop and improve cherry quality they can be treated with chemicals dried or healthier covered with rain shelters. to date the cracking phenomenon of cherries is still not clearly understood. therefore, in the ...200617390796
studies on water transport through the sweet cherry fruit surface. 11. fecl3 decreases water permeability of polar pathways.the effect of fecl3 (10 mm) on osmotic water uptake into detached sweet cherry fruit (prunus avium l.) and on the (3)h2o permeability (p(d)) of excised exocarp segments (es) or enzymatically isolated cuticular membranes (cm) was investigated. es or cm were mounted in an infinite dose diffusion system, where diffusion is monitored from a dilute donor solution through an interfacing es or cm into a receiver solution under quasi steady-state conditions. in the absence of fecl3, (3)h2o diffusion thr ...200616910722
inheritance and interactions of incompatibility alleles in the tetraploid sour cherry.three progenies of sour cherry (prunus cerasus) were analysed to correlate self-(in)compatibility status with s-rnase phenotype in this allotetraploid hybrid of sweet and ground cherry. self-(in)compatibility was assessed in the field and by monitoring pollen tube growth after selfing. the s-rnase phenotypes were determined by isoelectric focusing of stylar proteins and staining for rnase activity and, for the parents, confirmed by pcr. seedling phenotypes were generally consistent with disomic ...200616307228
studies on water transport through the sweet cherry fruit surface. 10. evidence for polar pathways across the exocarp.water uptake through the fruit surface is considered as an important factor in cracking of sweet cherry (prunus avium l.) fruit. uptake may occur by diffusion and/or viscous flow along a polar pathway. to establish the mechanism of water uptake, the effects of viscosity and molecular weight of selected osmotica on water uptake into detached sweet cherry fruit were investigated. in addition we investigated the effect of temperature on penetration of 2-(1-naphthyl)[1-(14)c]acetic acid ([(14)c]naa; ...200616719520
translocation of nitrogen in the xylem of field-grown cherry and poplar trees during remobilization.studies of small trees growing in pots have established that individual amino acids or amides are translocated in the xylem sap of a range of tree species following bud burst, as a consequence of nitrogen (n) remobilization from storage. this paper reports the first study of n translocation in the xylem of large, deciduous, field-grown trees during n remobilization in the spring. we applied 15n fertilizer to the soil around 10-year-old prunus avium l. and populus trichocharpa torr. & gray ex hoo ...200616414931
influence of cultivar and processing on cherry (prunus avium) allergenicity.oral allergy syndrome is an immediate food allergic event that affects lips, mouth, and pharynx, is often triggered by fruits and vegetables, and may be associated with pollinosis. here, we report on the allergenic pattern of different varieties of cherry (prunus avium) and results obtained by applying several technological processes to the selected varieties. whole cherries were submitted to chemical peeling, thermal treatment, and syruping processes, and the relative protein extracts were anal ...200617177523
variation in the composition of the heartwood flavonoids of prunus avium by on-column capillary gas chromatography.a fast and simple extraction procedure, coupled with on-column gas chromatography, has been developed in order to investigate the relative amounts of flavonoids in individual trees within several homogeneous populations of prunus avium. eight known flavonoid aglycones were isolated from the heartwood of p. avium and multivariate analysis was employed in order to characterise population groups.200614667064
scion-rootstock interaction affects the physiology and fruit quality of sweet cherry.water relations, leaf gas exchange, chlorophyll a fluorescence, light canopy transmittance, leaf photosynthetic pigments and metabolites and fruit quality indices of cherry cultivars 'burlat', 'summit' and 'van' growing on five rootstocks with differing size-controlling potentials that decrease in the order: prunus avium l. > cab 11e > maxma 14 > gisela 5 > edabriz, were studied during 2002 and 2003. rootstock genotype affected all physiological parameters. cherry cultivars grafted on invigorati ...200616203719
the mutated s1-haplotype in sour cherry has an altered s-haplotype-specific f-box protein gene.gametophytic self-incompatibility (gsi) is an outcrossing mechanism in flowering plants that is genetically controlled by 2 separate genes located at the highly polymorphic s-locus, termed s-haplotype. this study characterizes a pollen part mutant of the s(1)-haplotype present in sour cherry (rosaceae, prunus cerasus l.) that contributes to the loss of gsi. inheritance of s-haplotypes from reciprocal interspecific crosses between the self-compatible sour cherry cultivar ujfehértói fürtös carryin ...200616985081
self-incompatibility of prunus tenella and evidence that reproductively isolated species of prunus have different sfb alleles coupled with an identical s-rnase allele.many species of prunus display an s-rnase-based gametophytic self-incompatibility (si), controlled by a single highly polymorphic multigene complex termed the s-locus. this comprises tightly linked stylar- and pollen-expressed genes that determine the specificity of the si response. we investigated si of prunus tenella, a wild species found in small, isolated populations on the balkan peninsula, initially by pollination experiments and identifying stylar-expressed rnase alleles. nine p. tenella ...200717461794
composition of the cuticle of developing sweet cherry fruit.the composition of wax and cutin from developing sweet cherry (prunus avium) fruit was studied by gc-ms between 22 and 85 days after full bloom (dafb). in this and our previous study, fruit mass and surface area increased in a sigmoidal pattern with time, but mass of the cuticular membrane (cm) per unit fruit surface area decreased. on a whole fruit basis, mass of cm increased up to 36 dafb and remained constant thereafter. at maturity, triterpenes, alkanes and alcohols accounted for 75.6%, 19.1 ...200717328933
a new self-compatibility haplotype in the sweet cherry 'kronio', s5', attributable to a pollen-part mutation in the sfb gene.'kronio' is a sicilian cultivar of sweet cherry (prunus avium), nominally with the incompatibility genotype s(5)s(6), that is reported to be naturally self-compatible. in this work the cause of its self-compatibility was investigated. test selfing confirmed self-compatibility and provided embryos for analysis; pcr with consensus primers designed to amplify s-rnase and sfb alleles showed that the embryos were of two types, s(5)s(5) and s(5)s(6), indicating that s(6) pollen failed, but s(5) succee ...200718182436
potential for high hydrostatic pressure processing to control quarantine insects in fruit.tests were conducted to determine the potential for high hydrostatic pressure (hpp) to control codling moth, cydia pomonella (l.), and western cherry fruit fly, rhagoletis indifferens curran. apples (malus spp.) with codling moth larvae or eggs were treated at 24 and 72 h, respectively, after infestation at a series of pressures between 14,000 and 26,000 pounds per inch2 (psi). survivorship was determined the next day for larvae and after 10 d for eggs. codling moth eggs were more tolerant of hp ...200717972625
distribution and fine-scale spatial-genetic structure in british wild cherry (prunus avium l.).insights into the within-population spatial-genetic structure (sgs) of forest tree species, where little is known regarding seed and pollen dispersal patterns, enhance understanding of their ecology and provide information of value in conservation and breeding. this study utilised 13 polymorphic simple sequence repeat loci to investigate the impact of asexual recruitment, management regime and tree size on the development of sgs in wild cherry (prunus avium l). only 246 genotypes were identified ...200717245421
diurnal variation of remotely sensed et and some indices on sweet cherry trees in subhumid climate conditions.in this study, an experiment was conducted in sub-humid condition, several observations made to determine tc, spectral indexes, lwp and daily et of sweet cherry trees with different irrigation treatments (t1 and t2). the results showed that, tc-ta were increased from sunrise to noon and decreased from noon to sunset throughout the day on doy 171-172 (t1-t2) and doy 233-234 (t2). however, it was increased from sunrise to sunset on doy 233-234 for t1 treatment. it is very close the values of et(d) ...200719069947
self-compatible peach (prunus persica) has mutant versions of the s haplotypes found in self-incompatible prunus species.this study demonstrates that self-compatible (sc) peach has mutant versions of s haplotypes that are present in self-incompatible (si) prunus species. all three peach s haplotypes, s (1), s (2), and s (2m), found in this study encode mutated pollen determinants, sfb, while only s (2m) has a mutation that affects the function of the pistil determinant s-rnase. a cysteine residue in the c5 domain of the s (2m)-rnase is substituted by a tyrosine residue, thereby reducing rnase stability. the peach ...200717006593
effect of host plants on developmental time and life table parameters of amphitetranychus viennensis (acari: tetranychidae).the effect of host plant species including black cherry (prunus serotina cv. irani), cherry (prunus avium cv. siahe mashhad) and apple (malus domestica cv. shafi abadi) was studied on biological parameters of amphitetranychus viennensis (zacher) in the laboratory at 25 +/- 1 degrees c, 70 +/- 10% rh and 16l: 8d photoperiod. duration of each life stage, longevity, reproduction rate, the intrinsic rate of natural increase (rm), net reproductive rate (r0), mean generation time (t), doubling time (d ...200717710558
evaluation of evapotranspiration estimation methods for sweet cherry trees (prunus avium) in sub-humid climate.this study was carried out in the summer of 2001 in a 3 year old and in the summer of 2002 in a 4 year old sweet cherry trees (prunus avium, variety z-900) on mazzard rootstocks in bayramic-canakkale which is located in the west part of turkey. micro-sprinkler irrigation was selected as the irrigation method. the trees were subjected to four micro-sprinkler irrigation treatments (t-1, t-2, t-3 and t-4). the water applied in treatment t-3 was considered sufficient to satisfy fully needs of the cr ...200719069518
determination of antioxidant effects of some plant species wild growing in turkey.in this study, the antioxidant activity of 50% aqueous methanol extracts of crataegus tanacetifolia (lam.) pers, crataegus bornmuelleri zaberi, crataegus orientalis pall. ex m.bieb. var. orientalis, crataegus szovitsii pojark, crataegus curvisepala lindm., crataegus monogyna jacq. subsp. monogyna, crataegus monogyna jacq.subsp. azarella (gris.) franco, crataegus micophylla c.koch, rosa foetida herrm., rosa hemisphaerica j.herrm., rosa pulverulenta m.bieb., rosa canina l., rubus discolor weihe & ...200819382350
trans-specific s-rnase and sfb alleles in prunus self-incompatibility haplotypes.self-incompatibility in the genus prunus is controlled by two genes at the s-locus, s-rnase and sfb. both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. deduced polypeptide sequences of three myrobalan and three domestic plum s-rnases showed over 97% identity with s-rnases from other prunus species, including almond, sweet cherry, japanese apricot and japanese plum. the second intron, which is generally highly polymorphic bet ...200817989997
feeding substrates and behaviors of western cherry fruit fly (diptera: tephritidae).a study was conducted to determine the abundance of potential foods and the feeding substrates and behaviors of the western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), in 2005, 2006, and 2007 in central washington state. aphid colonies with honeydew, a presumed food source for flies, were not seen on randomly selected branches of sweet cherry trees, prunus avium l., but leaves with cherry juice, fruit that were damaged, and leaves with bird feces were commonly seen, e ...200818348808
evaluation of messenger plant activator as a preharvest and postharvest treatment of sweet cherry fruit under a controlled atmosphere.the preservation methods as an alternative to chemical control to prevent postharvest quality losses of sweet cherry were examined. the efficacy of preharvest and postharvest messenger (m) treatments on sweet cherry cv. '0900 ziraat' was tested under a controlled atmosphere in 2004 and 2005. the factors investigated included the separate or combined effect of low oxygen, high carbon dioxide and m on the quality and fungal pathogens of sweet cherries in a normal atmosphere (na) and in a controlle ...200818671163
separation of dna for molecular markers analysis from leaves of the vitis vinifera.in the present study, three dna extraction procedures were examined to determine which might yield dna from grape leaves suitable for molecular analysis for rapd, ssr. aflp and etc analysis. the three methods examined were: the miniprep procedure and the modified ctab for difficult species and protocol ctab. only the modified ctab method consistently yielded dna suitable for polymerase chain reaction (pcr) amplification, regardless of plant growing conditions or leaf age. the quality and quantit ...200818817243
functions of defense-related proteins and dehydrogenases in resistance response induced by salicylic acid in sweet cherry fruits at different maturity stages.we report here a comparative analysis of sweet cherry (prunus avium) fruits proteome induced by salicylic acid (sa) at different maturity stages. the results demonstrated that sa enhanced the resistance of sweet cherry fruits against penicillium expansum, resulting in lower disease incidences and smaller lesion diameters, especially at earlier maturity stage. based on proteomics analysis, 13 and 28 proteins were identified after sa treatment at earlier (a) and later (b) maturity stage, respectiv ...200818924108
characterization of a flowering cherry strain of cherry necrotic rusty mottle virus.the host range and complete nucleotide sequences of two cherry necrotic rusty mottle virus (cnrmv) isolates (fc4 and fc5) infecting flowering cherry accessions imported from japan are described. of the plants tested, cherry, peach, apricot and almond became infected, but only sweet cherry cv. 'canindex', nanking cherry and apricot cv. 'tilton' showed a mild foliar mottle. the genomic sequences of cnrmv-fc4 and cnrmv-fc5 are 8,430 and 8,429 nt in length, excluding the 3' poly (a) tail. they conta ...200818368286
reducing oxidative stress in sweet cherry fruit by pichia membranaefaciens: a possible mode of action against penicillium expansum.to investigate the effect of antagonistic yeast pichia membranaefaciens on alleviating oxidative stress caused by penicillium expansum in sweet cherry fruit.200818492044
functional characterization of palax1, a putative auxin permease, in heterologous plant systems.we have isolated the cdna of the gene palax1 from a wild cherry tree (prunus avium). the gene and its product are highly similar in sequences to both the cdnas and the corresponding protein products of aux/lax-type genes, coding for putative auxin influx carriers. we have prepared and characterized transformed nicotiana tabacum and arabidopsis thaliana plants carrying the gene palax1. we have proved that constitutive overexpression of palax1 is accompanied by changes in the content and distribut ...200818184737
seasonal amounts of nutrients in western cherry fruit fly (diptera: tephritidae) and their relation to nutrient availability on cherry plant surfaces.relatively little is known about the nutritional ecology of fruit flies in the genus rhagoletis. in this study, nutrient amounts in male and female western cherry fruit fly, rhagoletis indifferens curran, and availability of nitrogen and sugar on surfaces of leaves, fruit, and extrafloral nectaries (efns) of sweet cherry trees, were determined from late may to late june 2005 and of sugar from efns from mid-may to late june 2007 in washington state. protein amounts in male and female flies did no ...200819036186
unequal allelic frequencies at the self-incompatibility locus within local populations of prunus avium l.: an effect of population structure?in this paper, we investigated the genetic structure and distribution of allelic frequencies at the gametophytic self-incompatibility locus in three populations of prunus avium l. in line with theoretical predictions under balancing selection, genetic structure at the self-incompatibility locus was almost three times lower than at seven unlinked microsatellites. furthermore, we found that s-allele frequencies in wild cherry populations departed significantly from the expected isoplethic distribu ...200818284513
authentication of "cereza del jerte" sweet cherry varieties by free zone capillary electrophoresis (fzce).the purpose of this work was to develop a procedure based on protein analysis by free zone capillary electrophoresis (fzce) that can be used as an alternative to other methods in the determination of sweet cherry varieties for the authentication of "cereza del jerte". two autochthonous varieties of sweet cherry type "picota", 'ambrunés' and 'pico negro', and the foreign variety 'sweetheart' were used in the study. two protocols for extracting the methanol-soluble proteins were tested. on the bas ...200826047450
[effects of waterlogging on root respiration intensity and respiratory enzyme activities of sweet cherry].taking meizao/dongbeishanyingtao (prunus serrulata g. don) and meizao/mahaleb (p. mahaleb l.) as test materials, the respiration intensity and respiratory enzyme activities of their growing roots and brown lignified roots were determined under waterlogging. the results showed that under waterlogging, the root respiration intensity of the two kind rootstocks had a decreasing trend, with more decrement for their growing roots. for p. serrulata, the decrement of the respiration intensity of its gro ...200818839904
genetic and molecular characterization of three novel s-haplotypes in sour cherry (prunus cerasus l.).tetraploid sour cherry (prunus cerasus l.) exhibits gametophytic self-incompatibility (gsi) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, s-rnase and sfb (s haplotype-specific f-box protein gene), respectively. as sour cherry selections can be either self-compatible (sc) or self-incompatible (si), polyploidy per se does not result in sc. instead the genotype-dependent loss of si in sour cherry is due to the accumulation of n ...200818617504
[characteristics of urea 15n absorption, allocation, and utilization by sweet-cherry (prunus avium l.)].with five-year old 'zaodaguo' sweet-cherry (prunus avium l.) as test material, this paper studied the characteristics of its urea 15n absorption, allocation, and utilization when applied before bud-break. the results showed that the ndff of different organs increased gradually with time, and was higher in fine roots and storage organs at full-blooming stage. at fruit core-hardening stage, the ndff of long shoots and leaves increased quickly, reaching to 0.72% and 0.59% , respectively. from fruit ...200818533545
in vitro bioavailability of phenolic compounds from five cultivars of frozen sweet cherries (prunus avium l.).the bioavailability of phenolic compounds from five cultivars of frozen sweet cherries was assessed by a digestion process involving pepsin-hcl digestion (to simulate gastric digestion) and pancreatin digestion with bile salts (to simulate small intestine conditions) and dialyzed to assess serum- and colon-available fractions. after pepsin digestion, the % recovery of total phenolics, relative to the original starting material, increased, whereas the % anthocyanins did not change. following panc ...200818459792
mechanical stimuli regulate the allocation of biomass in trees: demonstration with young prunus avium trees.plastic tree-shelters are increasingly used to protect tree seedlings against browsing animals and herbicide drifts. the biomass allocation in young seedlings of deciduous trees is highly disturbed by common plastic tree-shelters, resulting in poor root systems and reduced diameter growth of the trunk. the shelters have been improved by creating chimney-effect ventilation with holes drilled at the bottom, resulting in stimulated trunk diameter growth, but the root deficit has remained unchanged. ...200818448448
effect of prunus avium roots on river bank strong.a pulling effect by side roots is one way in which roots help to side in-plane strong of a little depth soil mass. in contrast to the effect of vertically-enlarge roots, whereby soil is strengthened by an increase in its shear strength, the pulling effect strengthens the soil by increasing the tensile strength of the rooted soil zone. to verify whether or not a pulling effect exists in the root system of prunus avium in the roudsar, north iran and to study the importance and size of this effect, ...200818819551
chloroplast inheritance and dna variation in sweet, sour, and ground cherry.sour cherry (prunus cerasus l.) is an allotetraploid and both sweet cherry (p avium l.) and ground cherry (p. fruticosa pall.) are the proposed progenitor species. the study investigated the maternal species origin(s) of sour cherry using chloroplast dna (cpdna) markers and a diverse set of 22 sweet, 25 sour, and 7 ground cherry selections. two cpdna restriction fragment length polymorphisms (rflps) and one polymerase chain reaction (pcr) fragment length polymorphism were identified among the 54 ...200810739133
[acquisition and study of callus tissue during cultivation of mazzard and strawberry anthers].anters of 7 varieties of cerasus avium (2n=16) and fragaria grandiflora (2n==56) were cultured on murashige-skoog's medium. formation of the callus tissue was observed for only one variety of sweat cherry with a frequency of 3.1-20.6%. the number of haploid cells in first transfers varied from 22 to 44% in different strains. there were two rises of mitotic activity in the callus cells. subsequent transfers caused an increase in haploid cells. chromosome aberrations frequency was comparatively lo ...20081025784
basic rnases of wild almond (prunus webbii): cloning and characterization of six new s-rnase and one "non-s rnase" genes.in order to investigate the s-rnase allele structure of a prunus webbii population from the montenegrin region of the balkans, we analyzed 10 prunus webbii accessions. we detected 10 different s-rnase allelic variants and obtained the nucleotide sequences for six s-rnases. the blast analysis showed that these six sequences were new prunus webbii s-rnase alleles. it also revealed that one of sequenced alleles, s(9)-rnase, coded for an amino acid sequence identical to that for prunus dulcis s(14)- ...200918778875
diuretic effect of powdered cerasus avium (cherry) tails on healthy volunteers.in this study, the diuretic activity of powdered cherry stalk was evaluated in 13 healthy volunteers by means of their water balance. in addition to biochemical parameters, such as urinary electrolyte concentration, osmolality and any adverse reaction were determined. the capsules of cherry stalks were administered at an equivalent dose of 2.0 grams of the plant per person. urinary biochemical determination was made of concentration of electrolytes (sodium, potassium, chloride and calcium), urin ...200919783515
influence of habitat structure and resource availability on the movements of rhagoletis indifferens (diptera: tephritidae).habitat structure and resources availability may differentially influence movement between habitat patches. we examined fly movement decisions (stay or leave) at the scale of individual trees by measuring the response of marked rhagoletis indifferens (diptera: tephritidae) to sweet cherry trees (prunus avium) that were manipulated by changing the shape of the tree (structure treatment = normal or reduced) and the fruit load (fruit treatment = augmented, normal, or reduced). more than 600 observa ...200919508793
rootstock-induced dwarfing in cherries is caused by differential cessation of terminal meristem growth and is triggered by rootstock-specific gene regulation.use of dwarfing rootstocks has dramatically increased the profitability of fruit production by reducing production costs, reduced chemical use and higher density plantings. despite the importance of rootstock-induced dwarfing, the cause of this phenomenon is not known. using two commercially available graft combinations consisting of a sweet cherry scion, 'bing', on a dwarfing rootstock (gi5) or a semi-vigorous rootstock (gi6), we discovered that the difference in grafted tree height was due to ...200919429629
prunus avium: nuclear dna study in wild populations and sweet cherry cultivars.the pcr-ssr technique was used to detect nuclear dna diversity in five wild populations of prunus avium from deciduous forests in italy, slovenia, and croatia and 87 sweet cherry accessions from different geographical areas that have been maintained in the sweet cherry collection in italy. this sweet cherry collection includes local accessions from the campania region as well as accessions from different countries. twenty-eight microsatellites, previously developed in this species, generated pol ...200919370088
contemporary pollen flow, characterization of the maternal ecological neighbourhood and mating patterns in wild cherry (prunus avium l.).conversion of lowland woodland to agricultural land and resulting fragmentation in britain has been ongoing since neolithic times. to counteract this decline, plantations of native species, often based on non-british planting stock, have been established. this may ultimately be detrimental to the integrity of the native gene pool. we explore the genetic and ecological factors influencing the success of components of the local pollen pool, including the effect of a non-native planting on an ancie ...200919367315
maturity stage at harvest determines the fruit quality and antioxidant potential after storage of sweet cherry cultivars.eleven sweet cherry cultivars were harvested at three maturity stages (s1 to s3) based on skin color and stored at 2 degrees c for 16 days and a further period of 2 days at 20 degrees c (shelf life, sl) to analyze quality (color, total soluble solids, and total acidity) and bioactive compounds (total phenolics and anthocyanins) and their relationship to total antioxidant activity (taa), determined in hydrophilic (h-taa) or lipophilic (l-taa) fraction. for all cultivars and maturity stages, the r ...200919284725
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