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phenolic compounds profile and antioxidant properties of six sweet cherry (prunus avium) cultivars.sweet cherry (prunus avium) fruits are a nutritionally important food rich in dietary phenolic compounds. the aim of this study was to investigate the phenolic profile and chemometric discrimination of fruits from six cherry cultivars using a quantitative metabolomics approach, which combine non-targeted mass spectrometry and chemometric analysis. the assessment of the phenolic fingerprint of cherries allowed the tentative identification of 86 compounds. a total of 40 chlorogenic acids were iden ...028578036
sweet cherries from fundão possess antidiabetic potential and protect human erythrocytes against oxidative damage.cherries are one of the most appreciated summer fruits due to their attractive colour, sweet taste, high water content, low level of calories and composition in bioactive compounds which, in turn, are important to prevent some pathologies like diabetes, cardiovascular diseases and cancer. in this work we evaluated the phenolic profile and biological potential of 5 varieties of sweet cherries from fundão region (portugal) (saco, sweetheart, satin, maring and hedelfinger). a total of 23 phenolic c ...028395830
comparison of old cherry cultivars grown in czech republic by chemical composition and bioactive compounds.sweet cherry fruits are readily consumed by humans, and they contain significant quantities of bioactive compounds. fifteen sweet cherry (prunus avium l.) old cultivars grown in the czech republic were examined. in fresh fruits the contents of sugars, organic acids, and phenolic compounds, and antioxidant capacity were determined. the analyses showed that the fruits of sweet cherry are characterized by high contents of bioactive compounds. four organic acids - malic, quinic, shikimic, and fumari ...028317705
metabolomic and physico-chemical approach unravel dynamic regulation of calcium in sweet cherry fruit physiology.calcium (ca(2)) nutrition has a significant role in fruit physiology; however, the underlying mechanism is still unclear. in this study, fruit quality in response to cacl2, applied via foliar sprays (ca(2)) or/and hydro-cooling water (cahc), was characterized in 'lapins' cherries at harvest, just after cold storage (20 days at 0 °c) as well as after cold storage followed by 2 days at 20 °c, herein defined as shelf-life period. data indicated that pre- and post-harvest ca(2+) applications increas ...028551418
bioactive components of prunus avium l. black gold (red cherry) and prunus avium l. stark gold (white cherry) juices, wines and vinegars.cherries are one of the most popular fruits, characterized by attractive colour, firmness, appearance and delicious tastes. cherries are consumed fresh as well as in jams, wine, dried, candy and other processed products. cherries vary in antioxidant properties and phenolic substances. the aim of the study was to determine the effects of ethanol and acetic acid fermentation on total antioxidant activities and phenolic substances of cherry juice. total investigation of solids, ph, soluble solids, ...028242904
non-destructive sensor-based prediction of maturity and optimum harvest date of sweet cherry fruit.(1) background: the aim of the study was to use innovative sensor technology for non-destructive determination and prediction of optimum harvest date (ohd), using sweet cherry as a model fruit, based on different ripening parameters. (2) methods: two cherry varieties in two growing systems viz. field and polytunnel in two years were employed. the fruit quality parameters such as fruit weight and size proved unsuitable to detect ohd alone due to their dependence on crop load, climatic conditions, ...028146114
two minuses can make a plus: waterlogging and elevated co2 interactions in sweet cherry (prunus avium) cultivars.the increase in the ambient concentration of co2 and other greenhouse gases is producing climate events that can compromise crop survival. however, high co2 concentrations are sometimes able to mitigate certain stresses such as salinity or drought. in this experiment, the effects of waterlogging and co2 are studied in combination to elucidate the eventual response in sweet cherry trees. for this purpose, four sweet cherry cultivars ('burlat', 'cashmere', 'lapins, and 'new star') were grafted on ...028568609
co2 effects on the waterlogging response of 'gisela 5' and 'gisela 6' (prunus cerasusxprunus canescens) sweet cherry (prunus avium) rootstocks.climate change is submitting countries of the mediterranean arc to periods of drought alternating with heavy rain and waterlogging. eventual floods along with the rising co2 in the atmosphere present an unpredictable scenario that affects crop survival. the effect of both stresses combined has been studied in sweet cherry plants. 'gisela 5' and 'gisela 6' were evaluated as rootstocks of the sweet cherry cultivar 'burlat'. plants were placed in a controlled-climate chamber for 7days, then they we ...028407490
implication of abscisic acid on ripening and quality in sweet cherries: differential effects during pre- and post-harvest.sweet cherry, a non-climacteric fruit, is usually cold-stored during post-harvest to prevent over-ripening. the aim of the study was to evaluate the role of abscisic acid (aba) on fruit growth and ripening of this fruit, considering as well its putative implication in over-ripening and effects on quality. we measured the endogenous concentrations of aba during the ripening of sweet cherries (prunus avium l. var. prime giant) collected from orchard trees and in cherries exposed to 4°c and 23°c du ...027200070
impact of cultivar on profile and concentration of lipophilic bioactive compounds in kernel oils recovered from sweet cherry (prunus avium l.) by-products.lipophilic bioactive compounds in oils recovered from the kernels of seven sweet cherry (prunus avium l.) cultivars, harvested at single location in 2013, were studied. oil yield in sweet cherry ranged between 30.3-40.3 % (w/w) dw. the main fatty acids were oleic acid (39.62-49.92 %), linoleic acid (31.13-38.81 %), α-eleostearic acid (7.23-10.73 %) and palmitic acid (5.59-7.10 %), all four represented approximately 95 % of the total detected fatty acids. the ranges of total tocochromanols and st ...026984340
economic impact of the introduction and establishment of drosophila suzukii on sweet cherry production in switzerland.first detected in switzerland in 2011, the invasive drosophila suzukii, spotted wing drosophila, has caused recurring costs for growers of berries and fruit. recommended management approaches rely on a set of methods, tailored to suit crop requirements under the prevailing local conditions. control of d. suzukii represents a substantial economic burden for growers, in terms of material, equipment, new infrastructure and extra labour. however, those growers who invest wisely to deliver unblemishe ...028208692
wild prunus fruit species as a rich source of bioactive compounds.sugars, organic acids, carotenoids, tocopherols, chlorophylls, and phenolic compounds were quantified in fruit of 4 wild growing prunus species (wild cherry, bird cherry, blackthorn, and mahaleb cherry) using hplc-dad-msn. in wild prunus, the major sugars were glucose and fructose, whereas malic and citric acids dominated among organic acids. the most abundant classes of phenolic compounds in the analyzed fruit species were anthocyanins, flavonols, derivatives of cinnamic acids, and flavanols. t ...027464261
mismatch between cuticle deposition and area expansion in fruit skins allows potentially catastrophic buildup of elastic strain.the continuous deposition of cutin and wax during leaf and fruit growth is crucial to alleviate elastic strain of the cuticle, minimize the risk of failure and maintain its barrier functions. the cuticular membrane (cm) is a lipoidal biopolymer that covers primary surfaces of terrestrial plants. cms have barrier functions in water and solute transfer and pathogen invasion. these require intact cms throughout growth. this is a challenge particularly for fruit, because they increase in area from i ...027469168
preharvest salicylic acid and acetylsalicylic acid treatments preserve quality and enhance antioxidant systems during postharvest storage of sweet cherry cultivars.sweet cherries are much appreciated by consumers as a result of their organoleptic quality attributes and antioxidant properties, although they deteriorate rapidly after harvest. different preharvest strategies have been carried out to increase their quality at the time of harvest. we present data regarding the effect of preharvest salicylic acid (sa) and acetyl salicylic acid (asa) treatments on sweet cherry quality during postharvest storage.027312073
effect of nano-composite and thyme oil (tymus vulgaris l) coating on fruit quality of sweet cherry (takdaneh cv) during storage period.sweet cherry is one of the most appreciated fruit by consumers since it is an early season fruit and has an excellent quality. in this study effect of active nano composite formed from chitosan (as a matrix material), nano cellulose fiber (1% concentration) and thyme oils (tymus vulgaris l) at 1% concentration on fruits quality was investigated. treated fruits were stored at 1°c for 5 weeks and changes of different qualities attributes including weight loss, total acidity, tss, anthocyanin, tota ...026288725
the perfect stage of cylindosporium on prunus avium. 191317793207
tatter leaf of sweet cherry. 194621015648
passage of the ring spot virus through mazzard cherry seeds. 194617814230
[indeterminate chemistry of wood extracts. iv. new flavone derivatives from the heart of the wild cherry tree (prunus avium)]. 195413219528
[relation between the calcium and mineral phosphate content of the soil and the content of crude and elaborated sap of the wild cherry tree (prunus avium)]. 195513260867
[biochemistry of the sap of the wild cherry prunus avium]. 195513240336
[flavone compounds of wood extracts. v. water-soluble derivatives of sweet cherry heart (prunus avium)]. 195713437004
[isolation and determination of the structure of a new hydroxyflavanone from the cherry-tree heartwood (prunus avium)]. 195813608197
[biosynthesis of flavone derivatives in prunus avium. i. incorporation of potassium trans-3-c-14-cinnamate]. 195813596815
effect of selection on the frequency of genotypes determining incompatibility in prunus avium. 196013844941
possible virus-induced genetic abnormalities in tree fruits.seedlings of prunus avium and p. cerasus, grown from seed of infected trees, showed symptoms of cherry necrotic rusty mottle and sour cherry yellows diseases. no virus could be detected in the p. avium seedlings showing virus-like symptoms and only 10 percent of the 30 percent of p. cerasus seedlings showing symptoms contained virus. these results suggest that viruses may induce certain genetic abnormalities.196217836540
[biogenesis of plant pigments. ii. on the biogenesis of flavanones and flavanonols of robinia pseudoacacia, prunus avium and prunus mahaleb]. 19676056735
pharmacology of cherry (prunus avium) stalk extract. ii. cardiovascular effects. 19695791462
pharmacology of cherry (prunus avium) stalk extract. i. effect on smooth muscle. 19695791461
[the induction of an abscission layer in fruit of prunus avium l. by 2-chloroethylphosphonic acid].2-chloroethylphosphonic acid (cepa) facilitates the separation of the fruit from the pedicel significantly. the application of 2,000 and 4,000 ppm cepa in four sweet cherry varieties during maturation resulted in the formation of a complete abscission layer in the transition zone between pedicel and fruit. in contrast, in the untreated fruit no abscission layer was evident at maturity. the walls of the cells in the abscission layer contained less total polysaccharides than adjacent cells. cellul ...197024499842
a study of ethylene in apple, red raspberry, and cherry.high ethylene levels were associated with flower abscission in apple (malus sylvestris) and cherry (prunus avium and prunus cerasus), "june drop" of immature cherries, and harvest drop of apple and red raspberry (rubus idaeus). however, an increase in ethylene content was not associated with june drop of apples and harvest drop of cherries. during the period of fruit ripening on the plant, the largest increases in ethylene occurred in apple flesh and red raspberry receptacular tissue. ethylene r ...197216658013
the occurrence of nitrate reductase in leaves of prunus species.nitrate reductase was found in leaves of apricot prunus armeniaca, sour cherry p. cerasus, sweet cherry p. avium, and plum p. domestica, but not in peach p. persica, from trees grown in sand culture receiving a nitrate containing nutrient solution. nitrate was found in the leaves of all species. nitrate and nitrate reductase were found in leaves of field-grown apricot, sour cherry, and plum trees. the enzyme-extracting medium contained insoluble polyvinylpyrrolidone, and including dithiothreitol ...197216658037
inhibition by carboxylic acids of an o-diphenol oxidase from prunus avium fruits. 19744208079
ultrastructural localization of acid phosphatase in the pollen tube of prunus avium l. (sweet cherry).the pollen tube of prunus avium (cherry) consists of a growth zone of vesicles at the tip and an assemblage of organelles typical of an actively metabolizing cell. electron opaque globules are closely associated with the plasma membrane and fibrillar cell wall layer at the tip. acid phosphatase (ec 3.1.3.2) activity is localized in the membranes of 120 nm vesicles and er system, the lumen of 50 nm vesicles, the plasma membrane and the tube nucleus.197724420022
[about the influence of frost periods upon the serological detection of prunus ring spot viruses in cherries (author's transl)].during three years serological tests (latex test) were run from novemeber till april to detect prunus ring spot viruses in forced buds of prunus avium l., p. avium l. var. avium, and p. cerasus l. it was found that prunus necrotic ring spot virus (nrv) could be detected reliably during the winter in all infected trees. in contrary the detection of prune dwarf virus (pdv) was affected by temperatures below zero. in 1971 a low percentage of positive reacting trees was pointed out after the frost p ...1977910571
fluorine content of vegetables and fruits.twenty-six species of vegetables grown in szeged, in its environment and at szabadkigyós were examined for their f content. the mean f content of the most important staple products is 0.30 mg/kg raw weight or less. when vegetables of the same kind were examined, the results were mostly different. the highest mean f content was obtained for spinach (0.55 mg); potato yielded less (0.15 mg), and the lowest was obtained for cucumber (0.06 mg). the mean f content of the 13 fruit species was 0.10 mg/k ...1978754476
cytological zonation of prunus avium l. pollen tubes in vivo. 19807381990
tissue-specific antigens secreted by suspension-cultured callus cells of prunus avium l.a number of antigenic components are secreted into the medium by p. avium callus cells derived from different tissues and grown in suspension culture. these antigens have been detected using antiserum raised in rabbits to a protein fraction secreted by p. avium leaf callus. one antigen is specific to leaf tissue and is secreted by callus cells derived from stem, pistil and anthers as well as leaves. a second antigen is, in intact organs, restricted to styles of a particular self-incompatibility ...198124276761
style antigens of prunus avium l.antiserum to a protein fraction of an extract of mature styles of p. avium cv. lambert (s 3 s 4) was raised in rabbits. two major antigenic components of the style extracts were detected by immunoelectrophoresis and immunodiffusion. the presence of one antigen (s-antigen) correlated with a particular s-genotype (s 3 s 4). this antigen is restricted to mature styles of p. avium. the second antigen (p-antigen) was detected in styles of all prunus species examined, but not in styles of other specie ...198124276762
the effect of isolated components of prunus avium l. styles on in vitro growth of pollen tubes.a number of components isolated from styles of p. avium cv. napoleon (s 3 s 4) have been tested for their capacity to influence in vitro growth of pollen tubes from fresh and stored pollen (cv. napoleon (s 3 s 4)). an antigenic glycoprotein (antigen s) is a potent inhibitor of in-vitro pollen tube growth, causing a 65% reduction in tube length at a concentration of 20 μg/ml. none of the other style components were effective inhibitors of pollen tube growth; neither were proteins of animal origin ...198224272729
isolation and partial characterization of components of prunus avium l. styles, including an antigenic glycoprotein associated with a self-incompatibility genotype.several components of buffer extracts of prunus avium l. styles (cv. lambert, s 3 s 4) have been isolated and partially characterized: the major component is a glycoprotein (molecular weight approx. 90,000; 95% protein, 5.4% carbohydrate). a "sticky" uronic-acid-containing component and an arabinogalactan are also present. two minor components are an antigenic glycoprotein associated with the self-incompatibility genotype (antigen s) and a component found in styles of all prunus species (antigen ...198224272728
polyribosomes from aging apple and cherry fruit.the sequence of events which occurs during the ripening of the passe-crassane pear fruit have been previously studied. in this work, we have investigated the ripening of another climacteric fruit (pyrus malus l. cv golden delicious) and of a nonclimacteric fruit (prunus avium l. cv bigarreau napoléon). we show that both climacteric fruits exhibit the same preclimacteric sequence of events. differences exist, however, between the golden delicious apple and the passe-crassane pear in that the prot ...198316663295
plant virus detection using a new form of indirect elisa.a novel form of indirect enzyme-linked immunosorbent assay (elisa) has been devised for the detection of viruses in plants. the method uses protein a in two applications to sandwich antibody-antigen-antibody layers. the first applied layer of protein a prepares the plate for the coating antibody layer. the second layer of protein a is conjugated to the enzyme and detects the second antibody layer. the orientation of the igg induced in the coating layer of antibody prevents later unwanted reactio ...19854055975
spatial heterogeneity in post-dispersal predation on prunus and uvularia seeds.we investigated effects of seed density, distance from parent, and habitat (woods, open field) on post-dispersal predation risk (chiefly by rodents) for seeds of prunus virginiana (rosaceae). additional study of the habitat effect (woods, open field, treefall gap) was made with seeds of prunus avium (rosaceae) and uvularia grandiflora (liliaceae). density of prunus seeds (range 2-40 seeds/group) did not affect predation risk for individual seeds. distance from parent plants did influence predati ...198528309862
outer membrane protein mediating iron uptake via pyoverdinpss, the fluorescent siderophore produced by pseudomonas syringae pv. syringae.in an iron-limited environment pseudomonas syringae pv. syringae b301d produces a yellow-green fluorescent siderophore called pyoverdinpss which functions in high-affinity iron transport. two-dimensional electrophoretic comparisons of the outer membrane proteins of strain b301d identified nine proteins which were expressed at low (50 nm) but not at high (10 microm) iron concentrations. except for the minor protein 8e, the iron-regulated proteins exhibited high molecular weights ranging from appr ...19873032911
evaluation of the role of syringomycin in plant pathogenesis by using tn5 mutants of pseudomonas syringae pv. syringae defective in syringomycin production.syringomycin is a necrosis-inducing phytotoxin produced by pseudomonas syringae pv. syringae. to determine whether syringomycin production is a determinant in virulence or pathogenicity, we isolated nontoxigenic (tox) tn5-containing mutants and then quantitatively evaluated them for the ability to multiply and cause disease in immature sweet-cherry fruits. transposon tn5 was delivered to tox strain b301d-r by using the suicide vector, pgs9, and the resultant kanamycin-resistant (km) colonies wer ...198816347644
development, distribution, and characteristics of intrinsic, nonbacterial ice nuclei in prunus wood.ice nuclei active at approximately -2 degrees c and intrinsic to woody tissues of prunus spp. were shown to have properties distinct from bacterial ice nuclei. soaking 5-centimeter peach stem sections in water for 4 hours lowered the mean ice nucleation temperature to below -4 degrees c, nearly 2 degrees c lower than stems inoculated with ice nucleation-active pseudomonas syringae strain b301d. ice nucleation activity in peach was fully restored by air-drying woody stem sections for a few hours. ...198816666404
morphological variation in xiphinema spp. from new york orchards.xiphinema specimens were collected from orchards in southeastern, northeastern, and western new york. total length, distance of vulva from anterior end, spear length (odontostyle plus odontophore), body diameter at vulva, tail length, anal body diameter, and length and diameter of hyaline tail tip were measured on fixed, glycerol-infiltrated adult females. most specimens were identified as x. americanum or x. rivesi, but one western new york population was identified as x. californicum (a new re ...198819290184
long-term effect of electroporation on enhancement of growth and plant regeneration of colt cherry (prunus avium × pseudocerasus) protoplasts.electric pulses applied to colt cherry protoplasts enhanced the long-term growth and plant regeneration of protoplast-derived tissues. protoplasts isolated from long-term cultured tissues derived from electroporated protoplasts retained the ability to enter division in culture earlier and with a higher frequency of plant regeneration than untreated cell suspension protoplasts.198824240252
inhibition of growth and xylogenesis and promotion of vacuolation in prunus callus by the flavanone prunin.callus tissue of prunus avium l. responded to supplied prunin (naringen in 7-glucoside) showing vaculoation and storage of oligomeric proanthocyanidins. in addition, prunin caused restricted callus initiation and/or less callus growth. when prunin was omitted from the medium numerous tracheids and more peroxidases were formed in the callus.198824241596
cell wall synthesis and salt (saline) sensitivity in cultured colt cherry (prunus avium × pseudocerasus) protoplasts.in order to investigate the cellular basis of salt tolerance, colt cherry (prunus avium ×pseudocerasus) protoplasts from mesophyll tissues and root cell suspension cultures were cultured in the presence of nacl, kcl or na2so4, at normalities of 25, 50, 100 or 200 mn for each salt and with or without 2,6-dichlorobenzonitrile, an inhibitor of cell wall synthesis. results showed that the acquisition of salt tolerance was concomitant with the onset of cell wall regeneration, with protoplasts exhibit ...198924233277
selection for salt and drought tolerance in protoplast- and explant-derived tissue cultures of colt cherry (prunus avium x pseudocerasus).colt cherry (prunus avium x pseudocerasus) callus cultures were derived from leaf protoplasts, protoplasts of root cell suspension cultures, or by direct culture of leaf and root tissues. survival of calli cultured on basal proliferation medium containing 25, 50, 100 or 200 mn (millinormal) nacl, na(2)so(4) or kcl, or iso-osmotic (with nacl) concentrations of mannitol ranged from 1 to 15%. after six transfers on the same medium, surviving cell lines were subjected to three cycles of direct recur ...198914972993
somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (pyrus communis var. pyraster l.) and colt cherry (prunus avium x pseudocerasus).mesophyll protoplasts of wild pear (pyrus communis var. pyraster l., pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock colt (prunus avium x pseudocerasus, prunoideae), following an electroporation treatment of the separate parental protoplast systems. fusion-treated protoplasts were cultured, on modified k8p medium, where it had been previously established that neither parental protoplasts were capable of division. somatic hybrid calli were recovered and, foll ...198924227027
air pollutants from hydrocarbons and derivatives in micropropagation laboratories: toxicity symptoms on tissue culture of the cherry rootstock colt (prunus avium x p. pseudocerasus).several air pollutants in research and micropropagation laboratories originate from the combustion of hydrocarbons and their derivatives. the combustion products of some natural gases (propane-butane, propane, methane) and ethanol were analyzed, and the atmosphere composition was investigated inside the laminar flow box, inside the room where transplanting is performed and inside the culture vessels after transplanting. large quantities of ethylene and other biologically active compounds are pro ...199024227058
tests for transmission of prunus necrotic ringspot and two nepoviruses by criconemella xenoplax.in two of three trials, detectable color reactions in elisa for prunus necrotic ringspot virus (pnrsv) were observed for criconemella xenoplax handpicked from the root zone of infected peach trees. criconemella xenoplax (500/pot) handpicked from root zones of peach trees infected with pnrsv failed to transmit the virus to cucumber or peach seedlings. the nematode also failed to transmit tomato ringspot (tomrsv) or tobacco ringspot viruses between cucumbers, although xiphinema americanum transmit ...199019287748
[in vitro regeneration and applications using vegetable cell and tissue culture].plant cells by means of their totipotency and aided by in vitro culture techniques can be induced to perform morphogenesis leading to somatic embryoids and massive clonal multiplication; microspores or pollen can be triggered to recover haploid plants, then characters expressed via haploidy can be selected and fixed. protoplasts from different species can lead to recombinations. we report here work done on carica pubescens, where somatic embryoids were obtained from cells; in prunus avium androg ...19902133516
an alternative approach for gene transfer in trees using wild-type agrobacterium strains.micropropagated shoots of three forest tree species, poplar (populus tremula x p. alba), wild cherry (prunus avium l.) and walnut (juglans nigra x j. regia), were inoculated each with six different wild-type agrobacterium strains. poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. on poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic t-d ...19911653060
[analytic and biological standardization of prunus avium extracts].novicardine, the acetonic extract of p. avium peduncle which has advantageous cardiotonic effect, was standardized chemically and biologically. flavonones, flavones, isoflavones and their glycosides were (methanolbuffer solution eluent, gradient elution) detected by reverse phase hplc technique. it has been found that substances produced from different varieties of cherry peduncle originated from various lands, have the same components and their ratios are similar, but a contradictory statement ...19921442096
localization and quantitative determination of catechins and proanthocyanidins in the phloem of elm and cherry.the localization of catechins and proanthocyanidins in the phloem of elm (ulmus minor mill.) and cherry (prunus avium l.) was determined histochemically by use of 4-dimethylaminocinnamaldehyde (dmaca) reagent. the two tree species showed a characteristic distribution of these phenols, which were most abundant in the phloem rays of cherry, but were largely confined to upright parenchyma cells of elm. quantitative determination and qualitative separation of catechins and proanthocyanidins were per ...199214969867
sweet cherry cultivar identification by leaf isozyme polymorphism.isozyme polymorphism can assist in the identification of cherry cultivars. ten isozymes, each showing variation at only one locus, provide 70 unique genotype profiles from leaf extracts of 78 different sweet cherry cultivars. polymorphism in 6pgd, g6pd, gpi, idh, pgm, fdp, skdh and per is demonstrated for the first time, while observations are extended for the previously described polymorphisms in mdh and got. some cultivars with identical morphological characters and previously treated as one c ...199324193593
anti-inflammatory effects of cherry (prunus avium l.) stalk extract. 19948073068
rubisco and pep carboxylase responses to changing irradiance in a brazilian cerrado tree species, qualea grandiflora mart. (vochysiaceae).the activities of ribulose-1,5-bisphosphate carboxylase-oxygenase, rubisco (e.c. 4.1.1.39) and phosphoenolpyruvate carboxylase, pepc (e.c. 4.1.1.31), and concentrations of protein and chlorophyll were measured in extracts from cotyledons and first leaves of qualea grandiflora mart. (vochysiaceae) seedlings after transfer from high-light (20 days at 320 micro mol m(-2) s(-1), par) to low-light (35 days at 120 micro mol m(-2) s(-1), par) conditions. when tween 20 and glycerol were added to the ext ...199414967711
effects of elevated co(2) on growth and chloroplast proteins in prunus avium.to predict the future carbon sequestering capacity of trees, we need information about the possible acclimatory mechanisms of plant growth and photosynthesis in rising atmospheric co(2) under a variety of environmental conditions. we have, therefore, studied the growth response of a tree species (prunus avium l. stella (wild cherry)) to elevated co(2) and characterized the associated changes in photosynthetic machinery of the leaf tissue. self-pollinated seedlings and mature cuttings (clones) fr ...199414967647
plant-parasitic nematodes associated with cherry rootstocks in michigan.in two field trials, 10-year-old sweet and tart cherry rooted on 'mazzard', 'mahaleb', 'mxm 2', 'mxm 14', 'mxm 39', 'mxm 60', 'mxm 97', and 'colt' showed 10-203 pratylenchus penetrans per g fresh root from all tart rootstocks, and up to 46 pratylenchus, criconemella, and xiphinema spp. per 100 cm(3) soil. infestation of soil containing 1-year-old mazzard, mahaleb, mxm 60, 'gi148-1', and 'g1148-8' with 625/100 cm(3) soil of either p. penetrans or c. xenoplax resulting in final nematode population ...199419279962
analysis of sweet cherry (prunus avium l.) leaves for plant signal molecules that activate the syrb gene required for synthesis of the phytotoxin, syringomycin, by pseudomonas syringae pv syringae.an important aspect of the interaction of pseudomonas syringae pv syringae with plant hosts is the perception of plant signal molecules that regulate expression of genes, such as syrb, required for synthesis of the phytotoxin, syringomycin. in this study, the leaves of sweet cherry (prunus avium l.) were analyzed to determine the nature of the syrb-inducing activity associated with tissues of a susceptible host. crude leaf extracts yielded high amounts of total signal activity of more than 12,00 ...199512228385
cherry virus a: cdna cloning of dsrna, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry.the nucleotide sequence (7383 nucleotides) of a newly identified member of the genus capillovirus, cherry virus a (cva), was obtained from cdna clones. the cdna was generated from dsrna extracted from plant tissue infected with little cherry virus (lcv). small amounts of lcv dsrna served as template nucleic acid and enabled the construction of a library of which, unexpectedly, 7.5% of the recombinant plasmids were specific for cva. the genome organization of cva resembles that of apple stem groo ...19957636482
isozyme diversity in sour, sweet, and ground cherry.thirty-six sour (prunus cerasus l.), sweet (p. avium l.), and ground cherry (p. fruticosa pall.) selections were evaluated for seven enzyme systems and principal coordinate analysis was used to examine isozyme divergence among these cherry species. the enzyme systems studied were phosphoglucose isomerase (pgi), isocitrate dehydrogenase (idh), phosphoglucomutase (pgm), 6-phosphogluconate dehydrogenase (6-pgd), leucine aminopeptidase (lap), shikimate dehydrogenase (skdh), and malate dehydrogenase ...199524172928
a cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like.a 29-kd polypeptide is the most abundant soluble protein in ripe cherry fruit (prunus avium l); accumulation begins at the onset of ripening as the fruit turns from yellow to red. this protein was extracted from ripe cherries and purified by size-exclusion and ion-exchange chromatography. antibodies to the purified protein were used to screen a cdna library from ripe cherries. numerous recombinant plaques reacted positively with the antibodies; the dna sequence of representative clones encoded a ...19968685266
inheritance and linkage of isozymes in sweet cherry (prunus avium l.).eight polymorphic isozyme loci, 6pgd, g6pd, mdh, pgm, skdh, fdp, got and idh, in sweet cherry where found to be in one linkage group, with a ninth isozyme locus, gpi, being in another linkage group on a different chromosome. isozymes were also linked to the incompatibility s locus and this explained the disturbed segregation ratios observed in the first generation from controlled hybridisations between different sweet cherry cultivars. analysis revealed close linkage between the isozyme and s lo ...199624162301
photoperiod effect on bud burst in prunus is phase dependent: significance for early photosynthetic development.a 16-h photoperiod stimulated bud burst in mature rooted cuttings of prunus avium cv. stella, but not in selfed stella seedlings. however, in a 12-h photoperiod, bud burst occurred earlier in the seedlings than in the mature cuttings. in the 12-h photoperiod, production of polypeptides involved in carbon dioxide fixation and photosynthetic electron transport was higher in seedlings than in mature cuttings, whereas in the 16-h photoperiod, shoot development and polypeptide production were similar ...199614871718
molecular cloning, expression and characterization of pru a 1, the major cherry allergen.a high percentage of birch pollen allergic patients experiences food hypersensitivity reactions after ingestion of several fruits and vegetables. previous work demonstrated common epitopes on an allergen of mr 18,000 from sweet cherry (prunus avium) and bet v 1, the major allergen from birch pollen. n-terminal amino acid sequencing showed a sequence identity of 67% with bet v 1. here we report the cloning and cdna sequencing of this cherry allergen. the entire deduced amino acid sequence describ ...19979393965
impact of plant nutrition on pratylenchus penetrans infection of prunus avium rootstocks.a hypothesis that cherry rootstocks grown under optimal nutrient conditions are affected less by pratylenchus penetrans infection than those grown under deficient nutrient conditions was tested by growing four prunus avium l. rootstocks ('mazzard', 'mahaleb', 'gi148-1', and 'gi148-8') at a soil ph of 7.0 over a period of 3 months under greenhouse conditions (25 ñ 2 degrees c). pratylenchus penetrans was inoculated at 0 (control) or 1,500 nematodes per g fresh root weight for a total of 3,600, 4, ...199719274171
effects of elevated co(2) on chloroplast components, gas exchange and growth of oak and cherry.specific chloroplast proteins, gas exchange and dry matter production in oak (quercus robur l.) seedlings and clonal cherry (prunus avium l. x pseudocerasus lind.) plants were measured during 19 months of growth in climate-controlled greenhouses at ambient (350 vpm) or elevated (700 vpm) co(2). in both species, the elevated co(2) treatment increased the ppfd saturated-rate of photosynthesis and dry matter production. after two months at elevated co(2), prunus plants showed significant increases ...199714759855
physicochemical characteristics of selected sweet cherry cultivars.the volatile, sugar, and organic acid constituents in 12 cultivars and selections of sweet cherries (prunus avium l.) were characterized and quantified by high-performance liquid chromatography and gas chromatography (gc). fruit weight, soluble solids concentration (ssc), ph, titratable acidity (ta), and color (cie l, a, b) were also determined at harvest. weight ranged from 8.8 to 14.5 g per fruit, ssc from 13.5 to 24.5 degrees brix, and ssc/ta ratio from 18.3 to 29.0. chroma was a better indic ...199810554265
biochemical characterization of pru a 2, a 23-kd thaumatin-like protein representing a potential major allergen in cherry (prunus avium).the prevalence of allergy to fruits and vegetables increased with pollinosis over the last 10 years. so far, clusters of hypersensitivity have been established and corroborated by the molecular characterization of individual cross-reacting allergens. several case studies demonstrated the existence of allergic reactions to fruits of the subfamily prunoideae (apricots, cherries, plums and peaches). here, we present the characterization of a major allergen in cherry.19989623505
cytological comparison of leaves and stems of prunus avium l. shoots cultured on a solid medium with agar or gelrite.an axillary proliferating clone of prunus avium l. was subcultured every four weeks on solid ms medium with agar as the gelling agent. vitrification (hyperhydricity) of shoots was induced in one four week cycle with the same medium except that agar was replaced by gelrite. during culture on the vitrifying medium, the water content of the shoots progressively increased with a parallel decrease in chlorophyll content. cytological differences between the leaves and stems of the vitrified and normal ...19989554582
differences in root longevity of some tree species.although the importance of root production and mortality to nutrient fluxes in ecosystems is widely recognized, the difficulties associated with root measurements have limited the availability of reliable data. we have used minirhizotrons and image analysis to measure root longevity of prunus avium l., picea sitchensis (bong.) carrière, acer pseudoplatanus l. and populus x canadensis cv. beaupre directly in cohorts of roots. major differences in the longevity of roots among species were identifi ...199812651380
detection of plum pox virus by enzyme-linked immunosorbent assay in some apricot and peach varieties and hybrids in romania.plum pox virus (ppv) is a potyvirus widely spread in many species of the prunus genus such as plum, apricot, peach, sweet cherry and others. this potyvirus causes great damage to stone fruit trees in romania and other european countries as hungary, italy, czech republic, france, spain, greece, turkey, and slovak republic. the research station for fruit tree growing baneasa in bucharest has realized many studies on the epidemiology and spread of ppv and also on the disease symptomatology and dete ...199810073239
virulence and molecular polymorphism of prunus necrotic ringspot virus isolates.prunus necrotic ringspot virus (pnrsv) occurs as numerous strains or isolates that vary widely in their pathogenic, biophysical and serological properties. prior attempts to distinguish pathotypes based upon physical properties have not been successful; our approach was to examine the molecular properties that may distinguish these isolates. the nucleic acid sequence was determined from 1.65 kbp rt-pcr products derived from rna 3 of seven distinct isolates of pnrsv that differ serologically and ...19989680147
differentiation of closely related but biologically distinct cherry isolates of prunus necrotic ringspot virus by polymerase chain reaction.prunus necrotic ringspot ilarvirus (pnrsv) exists as a number of biologically distinct variants which differ in host specificity, serology, and pathology. previous nucleotide sequence alignment and phylogenetic analysis of cloned reverse transcription-polymerase chain reaction (rt-pcr) products of several biologically distinct sweet cherry isolates revealed correlations between symptom type and the nucleotide and amino acid sequences of the 3a (putative movement protein) and 3b (coat protein) op ...199910471030
cross-reactivity and epitope analysis of pru a 1, the major cherry allergen.a high percentage of birch pollen allergic patients experiences food hypersensivity after ingestion of fresh fruits and vegetables. the cross-reactivity of the major allergens of sweet cherry (pru a 1), apple (mal d 1), pear (pyr c 1), celery tuber (api g 1) and carrot (dau c 1) is due to structural similarities which are reflected by high amino acid sequence identities with bet v 1a, the major birch pollen allergen. apart from a strong cross-reactivity to bet v 1a, ige inhibition experiments wi ...199910403481
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
purification, partial amino acid sequence and structure of the product of raucaffricine-o-beta-d-glucosidase from plant cell cultures of rauwolfia serpentina.plant cell suspension cultures of rauwolfia produce within 1 week approximately 250 nkat/l of raucaffricine-o-beta-d-glucosidase. a five step procedure using anion exchange chromatography, chromatography on hydroxylapatite, gel filtration and fplc-chromatography on mono q and mono p delivered in a yield of 0.9% approximately 1200-fold enriched glucosidase. a short protocol employing deae sepharose, tsk 55 s gel chromatography and purification on mono q gave a 5% recovery of glucosidase which was ...199910234858
phenology in central europe--differences and trends of spring phenophases in urban and rural areas.in order to examine the impacts of both large-scale and small-scale climate changes (urban climate effect) on the development of plants, long-term observations of four spring phenophases from ten central european regions (hamburg, berlin, cologne, frankfurt, munich, prague, vienna, zurich, basle and chur) were analysed. the objective of this study was to identify and compare the differences in the starting dates of the pre-spring phenophases, the beginning of flowering of the snowdrop (galanthus ...200010993559
gibberellins in seedlings and flowering trees of prunus avium l.extracts of acids from mature seeds, germinating seeds, first, second and third year seedlings as well as mature, flowering trees of sweet cherry (prunus avium l. cv. stella) were analysed by gas chromatography-mass spectrometry. the presence of the known gibberellins (gas) ga1 (1), ga3 (4), ga5 (7), ga8 (11), ga19 (14), ga20 (12), ga29 (13), ga32 (5), ga85 (2), ga86 (3) and ga87 (6) was confirmed by comparison of their mass spectra and kovats retention indices with those of standards or literat ...200010731033
early brown rot infections in sweet cherry fruit are detected by monilinia-specific dna primers.abstract visible and nonvisible quiescent infections of immature and mature fruit are an integral component of the disease cycle of brown rot of sweet cherry in california. detection of these infections is critical for developing efficient and efficacious fungicide management programs. the previously published dna amplification primers mfs3 and ns5 for the identification of monilinia fructicola were very specific in amplifying dna of m. fructicola only and not m. laxa. this primer set, however, ...200018944605
studies on water transport through the sweet cherry fruit surface: characterizing conductance of the cuticular membrane using pericarp segments.water conductance of the cuticular membrane (cm) of mature sweet cherry fruit (prunus avium l. cv. sam) was investigated by monitoring water loss from segments of the outer pericarp excised from the cheek of the fruit. segments consisted of epidermis, hypodermis and several cell layers of the mesocarp. segments were mounted in stainless-steel diffusion cells with the mesocarp surface in contact with water, while the outer cuticular surface was exposed to dry silica (22 +/- 1 degrees c). conducta ...200011219577
are hyperhydric shoots of prunus avium l. energy deficient?the content of oxidized and reduced pyridine nucleotides and some enzymatic activities of the oxidative pentose phosphate and glycolytic pathways were compared in normal (ns, growing on agar) and hyperhydric (hs, growing on gelrite) shoots of prunus avium l. after 4 weeks of in vitro culture. the chlorophyll fluorescence from leaves and the redox capacity of the plasma membrane (reduction of exogenously added ferricyanide) of both types of shoots were recorded. the pool of oxidized and reduced p ...200111337071
purification and characterisation of a beta-glucosidase abundantly expressed in ripe sweet cherry (prunus avium l.) fruit.a beta-glucosidase (beta-d-glucoside glucohydrolase, ec 3.2.1.21) was purified to homogeneity from ripe fruits of sweet cherry (prunus avium l.) by ammonium sulphate precipitation, ion exchange and size exclusion chromatography. the enzyme is a monomer with a molecular mass of approximately 68 kda and an acidic isoelectric point. n-terminal sequence analysis indicated that sweet cherry beta-glucosidase is related to other plant cyanogenic beta-glucosidases. substrate specificity studies revealed ...200111297776
a population genetic analysis of chloroplast dna in wild populations of prunus avium l. in europe.a population genetic study of chloroplast dna was carried out in 23 wild populations of prunus avium sampled from several european deciduous forests. an analysis of approx. 9% of the chloroplast genome detected mostly insertion-deletion mutations and one point mutation. in all, 16 haplotypes were detected. six haplotypes were shared by two or more populations and 10 were unique. one haplotype was present in 21 of the 23 populations and 161 of 211 individuals, which probably indicates its ancient ...200111737289
studies on water transport through the sweet cherry fruit surface: ii. conductance of the cuticle in relation to fruit development.water conductance of the cuticular membrane (cm) of sweet cherry (prunus avium l. cv. sam) fruit during stages ii and iii (31-78 days after full bloom, dafb) was investigated by gravimetrically monitoring water loss through segments of the exocarp. segments were mounted in stainless-steel diffusion cells, filled with 0.5 ml of deionized water and incubated for 8 h at 25 +/- 2 degrees c over dry silica. conductance was calculated by dividing the amount of water transpired per unit surface area an ...200111722129
the distribution of little cherry disease in germany: lchv1 and lchv2 in cultivars of sweet cherry and in some other species. 200112425030
ozone exposure thresholds and foliar injury on forest plants in switzerland.canton ticino in southern switzerland is exposed to some of the highest concentrations of tropospheric ozone in europe. during recent field surveys in canton ticino, foliar symptoms identical to those caused by ozone have been documented on native tree and shrub species. in europe, the critical ozone level for forest trees has been defined at an aot40 of 10 ppm.h o3 (10 ppm.h accumulated exposure of ozone over a threshold of 40 ppb) during daylight hours over a six-month growing season. the obje ...200111202736
metabolism of gibberellins a1 and a3 in fruits and shoots of prunus avium.isotope-labelled ga metabolites were identified by gc--ms, following hplc fractionation of extracts derived from fruits or shoots, that had been incubated with [2h]- and [3h]- ga1 or [2h]- and [3h]- ga3. ga1 (1) was converted into ga8 (10) by developing fruits and vegetative shoots of sweet cherry (prunus avium cv. 'stella'), while ga3 (4) was converted into ga3-isolactone (17). other metabolites of each ga were detected but were not identified unequivocally. these included a metabolite of ga1 ( ...200111198821
variation of gas exchange within native plant species of switzerland and relationships with ozone injury: an open-top experiment.gas exchange and ozone-induced foliar injury were intensively measured during a 6-day period in mid-august 1998 on leaves of acer pseudoplatanus, betula pendula, corylus avellana, fagus sylvatica, fraxinus excelsior, morus nigra, prunus avium, prunus serotina, rhamnus cathartica, and viburnum lantana at a forest nursery site in canton ticino, switzerland. plants were grown in four open plots (aa), four open-top chambers receiving carbon-filtered (cf) air, and four receiving non-filtered (nf) air ...200111383335
the contribution of syringopeptin and syringomycin to virulence of pseudomonas syringae pv. syringae strain b301d on the basis of sypa and syrb1 biosynthesis mutant analysis.sequencing of an approximately 3.9-kb fragment downstream of the syrd gene of pseudomonas syringae pv. syringae strain b301d revealed that this region, designated sypa, codes for a peptide synthetase, a multifunctional enzyme involved in the thiotemplate mechanism of peptide biosynthesis. the translated protein sequence encompasses a complete amino acid activation module containing the conserved domains characteristic of peptide synthetases. analysis of the substrate specificity region of this m ...200111277431
partial characterization of a closterovirus associated with apple mealybug-transmitted little cherry disease in north america.abstract little cherry disease (lchd) is a serious economic problem of sweet cherry production in western north america where apple mealybug is the principle vector. lchd is associated with a distinct species of double-stranded (ds) rna. in this study, filamentous virus particles were purified from lchd-infected trees and shown to contain single-stranded rna corresponding to the previously reported dsrna isolated from infected trees. the virus particles were characterized and were similar to mon ...200118943346
seasonal variation of monoterpene emission from malus domestica and prunus avium.emission rates of monoterpenes released by apple (malus domestica borkh) and cherry (prunus avium l.) were estimated at different phenological stages. these measurements employed a dynamic flow-through teflon chamber, sample collection onto cartridges filled with graphitized carbon and thermal desorption gas chromatography-mass spectrometry (gc-ms) for identification and quantification of the emitted volatiles. at full bloom the release of monoterpene hydrocarbons from cherry flowers was 1213 ng ...200111397434
cyclooxygenase inhibitory and antioxidant cyanidin glycosides in cherries and berries.anthocyanins from tart cherries, prunus cerasus l. (rosaceae) cv. balaton and montmorency; sweet cherries, prunus avium l. (rosaceae); bilberries, vaccinum myrtillus l. (ericaceae); blackberries, rubus sp. (rosaceae); blueberries var. jersey, vaccinium corymbosum l. (ericaceae); cranberries var. early black, vaccinium macrocarpon ait. (ericaceae); elderberries, sambucus canadensis (caprifoliaceae); raspberries, rubus idaeus (rosaceae); and strawberries var. honeoye, fragaria x ananassa duch. (ro ...200111695879
development of microsatellite markers in peach [ prunus persica (l.) batsch] and their use in genetic diversity analysis in peach and sweet cherry ( prunus avium l.).we report the sequence of 41 primer pairs of microsatellites from a ct-enriched genomic library of the peach cultivar 'merrill o'henry'. ten microsatellite-containing clones had sequences similar to plant coding sequences in databases and could be used as markers for known functions. for microsatellites segregating at least in one of the two prunus f(2) progenies analyzed, it was possible to demonstrate mendelian inheritance. microsatellite polymorphism was evaluated in 27 peach and 21 sweet che ...200212582570
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