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an alternative approach for gene transfer in trees using wild-type agrobacterium strains.micropropagated shoots of three forest tree species, poplar (populus tremula x p. alba), wild cherry (prunus avium l.) and walnut (juglans nigra x j. regia), were inoculated each with six different wild-type agrobacterium strains. poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. on poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic t-d ...19911653060
outer membrane protein mediating iron uptake via pyoverdinpss, the fluorescent siderophore produced by pseudomonas syringae pv. syringae.in an iron-limited environment pseudomonas syringae pv. syringae b301d produces a yellow-green fluorescent siderophore called pyoverdinpss which functions in high-affinity iron transport. two-dimensional electrophoretic comparisons of the outer membrane proteins of strain b301d identified nine proteins which were expressed at low (50 nm) but not at high (10 microm) iron concentrations. except for the minor protein 8e, the iron-regulated proteins exhibited high molecular weights ranging from appr ...19873032911
detection of plum pox virus by enzyme-linked immunosorbent assay in some apricot and peach varieties and hybrids in romania.plum pox virus (ppv) is a potyvirus widely spread in many species of the prunus genus such as plum, apricot, peach, sweet cherry and others. this potyvirus causes great damage to stone fruit trees in romania and other european countries as hungary, italy, czech republic, france, spain, greece, turkey, and slovak republic. the research station for fruit tree growing baneasa in bucharest has realized many studies on the epidemiology and spread of ppv and also on the disease symptomatology and dete ...199810073239
ozone exposure thresholds and foliar injury on forest plants in switzerland.canton ticino in southern switzerland is exposed to some of the highest concentrations of tropospheric ozone in europe. during recent field surveys in canton ticino, foliar symptoms identical to those caused by ozone have been documented on native tree and shrub species. in europe, the critical ozone level for forest trees has been defined at an aot40 of 10 ppm.h o3 (10 ppm.h accumulated exposure of ozone over a threshold of 40 ppb) during daylight hours over a six-month growing season. the obje ...200111202736
sodium sulphite inhibition of potato and cherry polyphenolics in nucleic acid extraction for virus detection by rt-pcr.phenolic compounds from plant tissues inhibit reverse transcription-polymerase chain reaction (rt-pcr). multiple-step protocols using several additives to inhibit polyphenolic compounds during nucleic acid extraction are common, but time consuming and laborious. the current research highlights that the inclusion of 0.65 to 0.70% of sodium sulphite in the extraction buffer minimizes the pigmentation of nucleic acid extracts and improves the rt-pcr detection of potato virus y (pvy) and potato leaf ...200211684310
metabolism of gibberellins a1 and a3 in fruits and shoots of prunus avium.isotope-labelled ga metabolites were identified by gc--ms, following hplc fractionation of extracts derived from fruits or shoots, that had been incubated with [2h]- and [3h]- ga1 or [2h]- and [3h]- ga3. ga1 (1) was converted into ga8 (10) by developing fruits and vegetative shoots of sweet cherry (prunus avium cv. 'stella'), while ga3 (4) was converted into ga3-isolactone (17). other metabolites of each ga were detected but were not identified unequivocally. these included a metabolite of ga1 ( ...200111198821
gibberellins in seedlings and flowering trees of prunus avium l.extracts of acids from mature seeds, germinating seeds, first, second and third year seedlings as well as mature, flowering trees of sweet cherry (prunus avium l. cv. stella) were analysed by gas chromatography-mass spectrometry. the presence of the known gibberellins (gas) ga1 (1), ga3 (4), ga5 (7), ga8 (11), ga19 (14), ga20 (12), ga29 (13), ga32 (5), ga85 (2), ga86 (3) and ga87 (6) was confirmed by comparison of their mass spectra and kovats retention indices with those of standards or literat ...200010731033
differentiation of closely related but biologically distinct cherry isolates of prunus necrotic ringspot virus by polymerase chain reaction.prunus necrotic ringspot ilarvirus (pnrsv) exists as a number of biologically distinct variants which differ in host specificity, serology, and pathology. previous nucleotide sequence alignment and phylogenetic analysis of cloned reverse transcription-polymerase chain reaction (rt-pcr) products of several biologically distinct sweet cherry isolates revealed correlations between symptom type and the nucleotide and amino acid sequences of the 3a (putative movement protein) and 3b (coat protein) op ...199910471030
in vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (prunus avium l.).regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. "schneiders", "sweetheart", "starking hardy giant", "kordia" and "regina" (prunus avium l.). the influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. both, dkw/ ...200515948004
cross-reactivity and epitope analysis of pru a 1, the major cherry allergen.a high percentage of birch pollen allergic patients experiences food hypersensivity after ingestion of fresh fruits and vegetables. the cross-reactivity of the major allergens of sweet cherry (pru a 1), apple (mal d 1), pear (pyr c 1), celery tuber (api g 1) and carrot (dau c 1) is due to structural similarities which are reflected by high amino acid sequence identities with bet v 1a, the major birch pollen allergen. apart from a strong cross-reactivity to bet v 1a, ige inhibition experiments wi ...199910403481
virulence and molecular polymorphism of prunus necrotic ringspot virus isolates.prunus necrotic ringspot virus (pnrsv) occurs as numerous strains or isolates that vary widely in their pathogenic, biophysical and serological properties. prior attempts to distinguish pathotypes based upon physical properties have not been successful; our approach was to examine the molecular properties that may distinguish these isolates. the nucleic acid sequence was determined from 1.65 kbp rt-pcr products derived from rna 3 of seven distinct isolates of pnrsv that differ serologically and ...19989680147
combination of antagonistic yeasts with two food additives for control of brown rot caused by monilinia fructicola on sweet cherry fruit.to evaluate beneficial effect of two food additives, ammonium molybdate (nh4-mo) and sodium bicarbonate (nabi), on antagonistic yeasts for control of brown rot caused by monilinia fructicola in sweet cherry fruit under various storage conditions. the mechanisms of action by which food additives enhance the efficacy of antagonistic yeasts were also evaluated.200616478490
natural and recombinant molecules of the cherry allergen pru av 2 show diverse structural and b cell characteristics but similar t cell reactivity.cherry allergy is often reported in the context of allergy to other fruits of the rosaceae family and pollinosis to trees because of cross-reactive allergens. allergic reactions to cherry are reported by 19-29% of birch pollen-allergic patients. pru av 2, identified as a thaumatin-like protein (tlp) from sweet cherry, was recognized by the majority of cherry-allergic patients in immunoblotting.200616499648
molecular cloning, expression and characterization of pru a 1, the major cherry allergen.a high percentage of birch pollen allergic patients experiences food hypersensitivity reactions after ingestion of several fruits and vegetables. previous work demonstrated common epitopes on an allergen of mr 18,000 from sweet cherry (prunus avium) and bet v 1, the major allergen from birch pollen. n-terminal amino acid sequencing showed a sequence identity of 67% with bet v 1. here we report the cloning and cdna sequencing of this cherry allergen. the entire deduced amino acid sequence describ ...19979393965
anti-inflammatory effects of cherry (prunus avium l.) stalk extract. 19948073068
cherry virus a: cdna cloning of dsrna, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry.the nucleotide sequence (7383 nucleotides) of a newly identified member of the genus capillovirus, cherry virus a (cva), was obtained from cdna clones. the cdna was generated from dsrna extracted from plant tissue infected with little cherry virus (lcv). small amounts of lcv dsrna served as template nucleic acid and enabled the construction of a library of which, unexpectedly, 7.5% of the recombinant plasmids were specific for cva. the genome organization of cva resembles that of apple stem groo ...19957636482
pharmacology of cherry (prunus avium) stalk extract. ii. cardiovascular effects. 19695791462
pharmacology of cherry (prunus avium) stalk extract. i. effect on smooth muscle. 19695791461
plant virus detection using a new form of indirect elisa.a novel form of indirect enzyme-linked immunosorbent assay (elisa) has been devised for the detection of viruses in plants. the method uses protein a in two applications to sandwich antibody-antigen-antibody layers. the first applied layer of protein a prepares the plate for the coating antibody layer. the second layer of protein a is conjugated to the enzyme and detects the second antibody layer. the orientation of the igg induced in the coating layer of antibody prevents later unwanted reactio ...19854055975
[in vitro regeneration and applications using vegetable cell and tissue culture].plant cells by means of their totipotency and aided by in vitro culture techniques can be induced to perform morphogenesis leading to somatic embryoids and massive clonal multiplication; microspores or pollen can be triggered to recover haploid plants, then characters expressed via haploidy can be selected and fixed. protoplasts from different species can lead to recombinations. we report here work done on carica pubescens, where somatic embryoids were obtained from cells; in prunus avium androg ...19902133516
[analytic and biological standardization of prunus avium extracts].novicardine, the acetonic extract of p. avium peduncle which has advantageous cardiotonic effect, was standardized chemically and biologically. flavonones, flavones, isoflavones and their glycosides were (methanolbuffer solution eluent, gradient elution) detected by reverse phase hplc technique. it has been found that substances produced from different varieties of cherry peduncle originated from various lands, have the same components and their ratios are similar, but a contradictory statement ...19921442096
passage of the ring spot virus through mazzard cherry seeds. 194617814230
[about the influence of frost periods upon the serological detection of prunus ring spot viruses in cherries (author's transl)].during three years serological tests (latex test) were run from novemeber till april to detect prunus ring spot viruses in forced buds of prunus avium l., p. avium l. var. avium, and p. cerasus l. it was found that prunus necrotic ring spot virus (nrv) could be detected reliably during the winter in all infected trees. in contrary the detection of prune dwarf virus (pdv) was affected by temperatures below zero. in 1971 a low percentage of positive reacting trees was pointed out after the frost p ...1977910571
molecular basis of pollen-related food allergy: identification of a second cross-reactive ige epitope on pru av 1, the major cherry (prunus avium) allergen.birch (betula verrucosa) pollen-associated food allergy is a well-characterized syndrome, which is due to the cross-reactivity of ige antibodies to homologous allergens in various foods. one crossreacting area on the major birch pollen allergen bet v 1 and its homologue in cherry (prunus avium) pru av 1 has already been identified. this is the so-called 'p-loop' region, which encompasses amino acid residues around position 45 and is found on the two virtually identical tertiary protein structure ...200515330760
rubisco and pep carboxylase responses to changing irradiance in a brazilian cerrado tree species, qualea grandiflora mart. (vochysiaceae).the activities of ribulose-1,5-bisphosphate carboxylase-oxygenase, rubisco (e.c. 4.1.1.39) and phosphoenolpyruvate carboxylase, pepc (e.c. 4.1.1.31), and concentrations of protein and chlorophyll were measured in extracts from cotyledons and first leaves of qualea grandiflora mart. (vochysiaceae) seedlings after transfer from high-light (20 days at 320 micro mol m(-2) s(-1), par) to low-light (35 days at 120 micro mol m(-2) s(-1), par) conditions. when tween 20 and glycerol were added to the ext ...199414967711
characterization of a flowering cherry strain of cherry necrotic rusty mottle virus.the host range and complete nucleotide sequences of two cherry necrotic rusty mottle virus (cnrmv) isolates (fc4 and fc5) infecting flowering cherry accessions imported from japan are described. of the plants tested, cherry, peach, apricot and almond became infected, but only sweet cherry cv. 'canindex', nanking cherry and apricot cv. 'tilton' showed a mild foliar mottle. the genomic sequences of cnrmv-fc4 and cnrmv-fc5 are 8,430 and 8,429 nt in length, excluding the 3' poly (a) tail. they conta ...200818368286
effects of elevated co(2) on growth and chloroplast proteins in prunus avium.to predict the future carbon sequestering capacity of trees, we need information about the possible acclimatory mechanisms of plant growth and photosynthesis in rising atmospheric co(2) under a variety of environmental conditions. we have, therefore, studied the growth response of a tree species (prunus avium l. stella (wild cherry)) to elevated co(2) and characterized the associated changes in photosynthetic machinery of the leaf tissue. self-pollinated seedlings and mature cuttings (clones) fr ...199414967647
photoperiod effect on bud burst in prunus is phase dependent: significance for early photosynthetic development.a 16-h photoperiod stimulated bud burst in mature rooted cuttings of prunus avium cv. stella, but not in selfed stella seedlings. however, in a 12-h photoperiod, bud burst occurred earlier in the seedlings than in the mature cuttings. in the 12-h photoperiod, production of polypeptides involved in carbon dioxide fixation and photosynthetic electron transport was higher in seedlings than in mature cuttings, whereas in the 16-h photoperiod, shoot development and polypeptide production were similar ...199614871718
mutational epitope analysis of pru av 1 and api g 1, the major allergens of cherry (prunus avium) and celery (apium graveolens): correlating ige reactivity with three-dimensional structure.birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific ige cross-reacting with homologous food allergens. the tertiary structure of pru av 1, the major cherry (prunus avium) allergen, for example, is nearly identical with bet v 1, the major birch (betula verrucosa) pollen allergen. in order to define cross-reactive ige epitopes, we generated and analysed mutants of pru av 1 and api g 1.0101, the major celery (apium graveolens) allergen, by immunoblotti ...200312943529
ozone air pollution and foliar injury development on native plants of switzerland.the objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. the study was conducted from the beginning of may through the end of august during 2000 and 2001 using an open-top chamber research facility located within the lattecaldo cantonal forest nursery in canton ticino, southern switzerland (600 m asl) ...200312804826
development of microsatellite markers in peach [ prunus persica (l.) batsch] and their use in genetic diversity analysis in peach and sweet cherry ( prunus avium l.).we report the sequence of 41 primer pairs of microsatellites from a ct-enriched genomic library of the peach cultivar 'merrill o'henry'. ten microsatellite-containing clones had sequences similar to plant coding sequences in databases and could be used as markers for known functions. for microsatellites segregating at least in one of the two prunus f(2) progenies analyzed, it was possible to demonstrate mendelian inheritance. microsatellite polymorphism was evaluated in 27 peach and 21 sweet che ...200212582570
analysis of sweet cherry (prunus avium l.) leaves for plant signal molecules that activate the syrb gene required for synthesis of the phytotoxin, syringomycin, by pseudomonas syringae pv syringae.an important aspect of the interaction of pseudomonas syringae pv syringae with plant hosts is the perception of plant signal molecules that regulate expression of genes, such as syrb, required for synthesis of the phytotoxin, syringomycin. in this study, the leaves of sweet cherry (prunus avium l.) were analyzed to determine the nature of the syrb-inducing activity associated with tissues of a susceptible host. crude leaf extracts yielded high amounts of total signal activity of more than 12,00 ...199512228385
using survival analysis to investigate the effect of uv-c and heat treatment on storage rot of strawberry and sweet cherry.ultraviolet light and heat treatment are proposed as alternative techniques for the use of chemicals to reduce the development of the spoilage fungi botrytis cinerea and monilinia fructigena on strawberry and sweet cherry, respectively, during storage. in order to investigate the effect of both physical techniques on microbial inactivation and on fruit quality, inoculated berries were subjected to different temperatures (40-48 degrees c) and uv-c doses (0.05-1.50 j/cm2). for each condition, 20 b ...200211934026
variation of gas exchange within native plant species of switzerland and relationships with ozone injury: an open-top experiment.gas exchange and ozone-induced foliar injury were intensively measured during a 6-day period in mid-august 1998 on leaves of acer pseudoplatanus, betula pendula, corylus avellana, fagus sylvatica, fraxinus excelsior, morus nigra, prunus avium, prunus serotina, rhamnus cathartica, and viburnum lantana at a forest nursery site in canton ticino, switzerland. plants were grown in four open plots (aa), four open-top chambers receiving carbon-filtered (cf) air, and four receiving non-filtered (nf) air ...200111383335
the contribution of syringopeptin and syringomycin to virulence of pseudomonas syringae pv. syringae strain b301d on the basis of sypa and syrb1 biosynthesis mutant analysis.sequencing of an approximately 3.9-kb fragment downstream of the syrd gene of pseudomonas syringae pv. syringae strain b301d revealed that this region, designated sypa, codes for a peptide synthetase, a multifunctional enzyme involved in the thiotemplate mechanism of peptide biosynthesis. the translated protein sequence encompasses a complete amino acid activation module containing the conserved domains characteristic of peptide synthetases. analysis of the substrate specificity region of this m ...200111277431
[identifying the s genotypes of sweet cherry (prunus avium l.) cultivars].this report identified s-rnase genes (s genes) of sweet cherry (prunus avium l.), presented the sequences of s genes by using a pair of specific primers pruc2 and pruc4r based on the conserved regions c2 and rc4 of rosaceous s-rnase genes and researched the s gene specific products from the genomic dnas of different cultivars in which most of the s genotypese were unknown. the bands of pcr were cloned and their sequences were compared in genbank. four s genes were defined and the conclusion was ...200415552051
characterization of pseudomonas pathovars isolated from rosaceous fruit trees in east algeria.a survey of bacterial diseases due to pseudomonas on rosaceous fruit trees was conducted. in forty two orchards located in the constantine region ( east algeria). pseudomonas isolates were identified on the bases of their cultural and biochemical characteristics . a total of fifty nine phytopathogenic bacteria were isolated from diseased pome and stone fruit trees. thirty one strains comparable to pseudomonas syringae pv. syringae were isolated from cherry (prunus avium l.), plum (p. domestica l ...200415756824
impact of plant nutrition on pratylenchus penetrans infection of prunus avium rootstocks.a hypothesis that cherry rootstocks grown under optimal nutrient conditions are affected less by pratylenchus penetrans infection than those grown under deficient nutrient conditions was tested by growing four prunus avium l. rootstocks ('mazzard', 'mahaleb', 'gi148-1', and 'gi148-8') at a soil ph of 7.0 over a period of 3 months under greenhouse conditions (25 ñ 2 degrees c). pratylenchus penetrans was inoculated at 0 (control) or 1,500 nematodes per g fresh root weight for a total of 3,600, 4, ...199719274171
plant-parasitic nematodes associated with cherry rootstocks in michigan.in two field trials, 10-year-old sweet and tart cherry rooted on 'mazzard', 'mahaleb', 'mxm 2', 'mxm 14', 'mxm 39', 'mxm 60', 'mxm 97', and 'colt' showed 10-203 pratylenchus penetrans per g fresh root from all tart rootstocks, and up to 46 pratylenchus, criconemella, and xiphinema spp. per 100 cm(3) soil. infestation of soil containing 1-year-old mazzard, mahaleb, mxm 60, 'gi148-1', and 'g1148-8' with 625/100 cm(3) soil of either p. penetrans or c. xenoplax resulting in final nematode population ...199419279962
influence of selected fruit tree pollen on life history of euseius stipulatus (acari: phytoseiidae).euseius stipulatus (athias-henriot) is a predatory mite widespread in the mediterranean region considered to be important for the biological control of spider mites in citrus orchards. development, survival and reproduction of this phytoseiid mite feeding on seven commercially obtained pollen were studied under constant laboratory conditions (20 +/- 1 degrees c, rh 65 +/- 5%, photoperiod 16l: 8d h). mites were kept individually at rearing units with ample quantity of almond (prunus amygdalus bat ...200516082919
evaluation of the role of syringomycin in plant pathogenesis by using tn5 mutants of pseudomonas syringae pv. syringae defective in syringomycin production.syringomycin is a necrosis-inducing phytotoxin produced by pseudomonas syringae pv. syringae. to determine whether syringomycin production is a determinant in virulence or pathogenicity, we isolated nontoxigenic (tox) tn5-containing mutants and then quantitatively evaluated them for the ability to multiply and cause disease in immature sweet-cherry fruits. transposon tn5 was delivered to tox strain b301d-r by using the suicide vector, pgs9, and the resultant kanamycin-resistant (km) colonies wer ...198816347644
a study of ethylene in apple, red raspberry, and cherry.high ethylene levels were associated with flower abscission in apple (malus sylvestris) and cherry (prunus avium and prunus cerasus), "june drop" of immature cherries, and harvest drop of apple and red raspberry (rubus idaeus). however, an increase in ethylene content was not associated with june drop of apples and harvest drop of cherries. during the period of fruit ripening on the plant, the largest increases in ethylene occurred in apple flesh and red raspberry receptacular tissue. ethylene r ...197216658013
the occurrence of nitrate reductase in leaves of prunus species.nitrate reductase was found in leaves of apricot prunus armeniaca, sour cherry p. cerasus, sweet cherry p. avium, and plum p. domestica, but not in peach p. persica, from trees grown in sand culture receiving a nitrate containing nutrient solution. nitrate was found in the leaves of all species. nitrate and nitrate reductase were found in leaves of field-grown apricot, sour cherry, and plum trees. the enzyme-extracting medium contained insoluble polyvinylpyrrolidone, and including dithiothreitol ...197216658037
development, distribution, and characteristics of intrinsic, nonbacterial ice nuclei in prunus wood.ice nuclei active at approximately -2 degrees c and intrinsic to woody tissues of prunus spp. were shown to have properties distinct from bacterial ice nuclei. soaking 5-centimeter peach stem sections in water for 4 hours lowered the mean ice nucleation temperature to below -4 degrees c, nearly 2 degrees c lower than stems inoculated with ice nucleation-active pseudomonas syringae strain b301d. ice nucleation activity in peach was fully restored by air-drying woody stem sections for a few hours. ...198816666404
bee population returns and cherry yields in an orchard pollinated with osmia lignaria (hymenoptera: megachilidae).during 1998-2003, we used populations of the solitary bee osmia lignaria say to pollinate a commercial sweet cherry orchard in northern utah. bee densities released each year ranged from 1290 to 1857 females/ha, with approximately twice as many males. female progeny produced each year were greater than parental populations released, except in 2003, when nesting was poor due to bird predation. despite poor weather during bloom, and in contrast to most other local producers, the study orchard prod ...200616686139
molecular characterization of three non-functional s-haplotypes in sour cherry (prunus cerasus).tetraploid sour cherry (prunus cerasus) exhibits a genotype-dependent loss of gametophytic self-incompatibility that is caused by the accumulation of non-functional s-haplotypes with disrupted pistil component (stylar-s) and/or pollen component (pollen-s) function. genetic studies using diverse sour cherry germplasm identified non-functional s-haplotypes for which an equivalent wild-type s-haplotype was present in sweet cherry (prunus avium), a diploid progenitor of sour cherry. in all cases, th ...200616915517
comparative analysis of the within-population genetic structure in wild cherry (prunus avium l.) at the self-incompatibility locus and nuclear microsatellites.gametophytic self-incompatibility (si) systems in plants exhibit high polymorphism at the si controlling s-locus because individuals with rare alleles have a higher probability to successfully pollinate other plants than individuals with more frequent alleles. this process, referred to as frequency-dependent selection, is expected to shape number, frequency distribution, and spatial distribution of self-incompatibility alleles in natural populations. we investigated the genetic diversity and the ...200616968267
self-compatible peach (prunus persica) has mutant versions of the s haplotypes found in self-incompatible prunus species.this study demonstrates that self-compatible (sc) peach has mutant versions of s haplotypes that are present in self-incompatible (si) prunus species. all three peach s haplotypes, s (1), s (2), and s (2m), found in this study encode mutated pollen determinants, sfb, while only s (2m) has a mutation that affects the function of the pistil determinant s-rnase. a cysteine residue in the c5 domain of the s (2m)-rnase is substituted by a tyrosine residue, thereby reducing rnase stability. the peach ...200717006593
effect of host plants on developmental time and life table parameters of amphitetranychus viennensis (acari: tetranychidae).the effect of host plant species including black cherry (prunus serotina cv. irani), cherry (prunus avium cv. siahe mashhad) and apple (malus domestica cv. shafi abadi) was studied on biological parameters of amphitetranychus viennensis (zacher) in the laboratory at 25 +/- 1 degrees c, 70 +/- 10% rh and 16l: 8d photoperiod. duration of each life stage, longevity, reproduction rate, the intrinsic rate of natural increase (rm), net reproductive rate (r0), mean generation time (t), doubling time (d ...200717710558
feeding substrates and behaviors of western cherry fruit fly (diptera: tephritidae).a study was conducted to determine the abundance of potential foods and the feeding substrates and behaviors of the western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), in 2005, 2006, and 2007 in central washington state. aphid colonies with honeydew, a presumed food source for flies, were not seen on randomly selected branches of sweet cherry trees, prunus avium l., but leaves with cherry juice, fruit that were damaged, and leaves with bird feces were commonly seen, e ...200818348808
exploitation of sweet cherry (prunus avium l.) puree added of stem infusion through fermentation by selected autochthonous lactic acid bacteria.strains of lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus and leuconostoc mesenteroides subsp. mesenteroides were identified from 8 cultivars of sweet cherry by partial 16s rrna gene sequence and subjected to typing by random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) analysis. representative isolates from each species and each cultivar were screened based on the kinetics of growth on cherry puree added of (10%, v/v) stem infusion (cp-si). a protoc ...201121569932
reducing oxidative stress in sweet cherry fruit by pichia membranaefaciens: a possible mode of action against penicillium expansum.to investigate the effect of antagonistic yeast pichia membranaefaciens on alleviating oxidative stress caused by penicillium expansum in sweet cherry fruit.200818492044
evaluation of messenger plant activator as a preharvest and postharvest treatment of sweet cherry fruit under a controlled atmosphere.the preservation methods as an alternative to chemical control to prevent postharvest quality losses of sweet cherry were examined. the efficacy of preharvest and postharvest messenger (m) treatments on sweet cherry cv. '0900 ziraat' was tested under a controlled atmosphere in 2004 and 2005. the factors investigated included the separate or combined effect of low oxygen, high carbon dioxide and m on the quality and fungal pathogens of sweet cherries in a normal atmosphere (na) and in a controlle ...200818671163
separation of dna for molecular markers analysis from leaves of the vitis vinifera.in the present study, three dna extraction procedures were examined to determine which might yield dna from grape leaves suitable for molecular analysis for rapd, ssr. aflp and etc analysis. the three methods examined were: the miniprep procedure and the modified ctab for difficult species and protocol ctab. only the modified ctab method consistently yielded dna suitable for polymerase chain reaction (pcr) amplification, regardless of plant growing conditions or leaf age. the quality and quantit ...200818817243
functions of defense-related proteins and dehydrogenases in resistance response induced by salicylic acid in sweet cherry fruits at different maturity stages.we report here a comparative analysis of sweet cherry (prunus avium) fruits proteome induced by salicylic acid (sa) at different maturity stages. the results demonstrated that sa enhanced the resistance of sweet cherry fruits against penicillium expansum, resulting in lower disease incidences and smaller lesion diameters, especially at earlier maturity stage. based on proteomics analysis, 13 and 28 proteins were identified after sa treatment at earlier (a) and later (b) maturity stage, respectiv ...200818924108
partial characterization of a closterovirus associated with apple mealybug-transmitted little cherry disease in north america.abstract little cherry disease (lchd) is a serious economic problem of sweet cherry production in western north america where apple mealybug is the principle vector. lchd is associated with a distinct species of double-stranded (ds) rna. in this study, filamentous virus particles were purified from lchd-infected trees and shown to contain single-stranded rna corresponding to the previously reported dsrna isolated from infected trees. the virus particles were characterized and were similar to mon ...200118943346
enhancement of biocontrol activity of cryptococcus laurentii by silicon and the possible mechanisms involved.abstract exogenous application of silicon (si) in the form of sodium metasilicate reduced disease development caused by penicillium expansum and monilinia fructicola in sweet cherry fruit at 20 degrees c. the inhibition of fruit decay was correlated closely with si concentrations. silicon at concentrations of 1%, in combination with the biocontrol agent cryptococcus laurentii at 1 x 10(7) cells per ml, provided synergistic effects against both diseases. population dynamics of c. laurentii were s ...200518943838
early brown rot infections in sweet cherry fruit are detected by monilinia-specific dna primers.abstract visible and nonvisible quiescent infections of immature and mature fruit are an integral component of the disease cycle of brown rot of sweet cherry in california. detection of these infections is critical for developing efficient and efficacious fungicide management programs. the previously published dna amplification primers mfs3 and ns5 for the identification of monilinia fructicola were very specific in amplifying dna of m. fructicola only and not m. laxa. this primer set, however, ...200018944605
evaluation of evapotranspiration estimation methods for sweet cherry trees (prunus avium) in sub-humid climate.this study was carried out in the summer of 2001 in a 3 year old and in the summer of 2002 in a 4 year old sweet cherry trees (prunus avium, variety z-900) on mazzard rootstocks in bayramic-canakkale which is located in the west part of turkey. micro-sprinkler irrigation was selected as the irrigation method. the trees were subjected to four micro-sprinkler irrigation treatments (t-1, t-2, t-3 and t-4). the water applied in treatment t-3 was considered sufficient to satisfy fully needs of the cr ...200719069518
diurnal variation of remotely sensed et and some indices on sweet cherry trees in subhumid climate conditions.in this study, an experiment was conducted in sub-humid condition, several observations made to determine tc, spectral indexes, lwp and daily et of sweet cherry trees with different irrigation treatments (t1 and t2). the results showed that, tc-ta were increased from sunrise to noon and decreased from noon to sunset throughout the day on doy 171-172 (t1-t2) and doy 233-234 (t2). however, it was increased from sunrise to sunset on doy 233-234 for t1 treatment. it is very close the values of et(d) ...200719069947
anthocyanin content, lipid peroxidation and cyclooxygenase enzyme inhibitory activities of sweet and sour cherries.cherries contain bioactive anthocyanins that are reported to possess antioxidant, anti-inflammatory, anticancer, antidiabetic and antiobese properties. the present study revealed that red sweet cherries contained cyanidin-3-o-rutinoside as major anthocyanin (>95%). the sweet cherry cultivar "kordia" (aka "attika") showed the highest cyanidin-3-o-rutinoside content, 185 mg/100 g fresh weight. the red sweet cherries "regina" and "skeena" were similar to "kordia", yielding cyanidin-3-o-rutinoside a ...200919199585
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
tests for transmission of prunus necrotic ringspot and two nepoviruses by criconemella xenoplax.in two of three trials, detectable color reactions in elisa for prunus necrotic ringspot virus (pnrsv) were observed for criconemella xenoplax handpicked from the root zone of infected peach trees. criconemella xenoplax (500/pot) handpicked from root zones of peach trees infected with pnrsv failed to transmit the virus to cucumber or peach seedlings. the nematode also failed to transmit tomato ringspot (tomrsv) or tobacco ringspot viruses between cucumbers, although xiphinema americanum transmit ...199019287748
determination of antioxidant effects of some plant species wild growing in turkey.in this study, the antioxidant activity of 50% aqueous methanol extracts of crataegus tanacetifolia (lam.) pers, crataegus bornmuelleri zaberi, crataegus orientalis pall. ex m.bieb. var. orientalis, crataegus szovitsii pojark, crataegus curvisepala lindm., crataegus monogyna jacq. subsp. monogyna, crataegus monogyna jacq.subsp. azarella (gris.) franco, crataegus micophylla c.koch, rosa foetida herrm., rosa hemisphaerica j.herrm., rosa pulverulenta m.bieb., rosa canina l., rubus discolor weihe & ...200819382350
simultaneous detection of cherry necrotic rusty mottle virus and cherry green ring mottle virus using real-time pcr and high resolution melting analysis.in this study, the real-time pcr assays were combined with high resolution melting (hrm) analysis for the simultaneous detection of cherry necrotic rusty mottle virus (cnrmv) and cherry green ring mottle virus (cgrmv) infection in sweet cherry trees. detection of cnrmv and cgrmv was performed in a real-time pcr using a primer set for both of them or duplex real-time pcr that included one specific primer set for each virus. these two strategies allowed us to confirmed virus infection in all teste ...201424675146
'kwanzan stunting' syndrome: detection and molecular characterization of an italian isolate of little cherry virus 1.evident stunting was observed for the first time on prunus serrulata 'kwanzan' indicator trees in southern italy during the indexing of two sour cherry accessions from cultivars 'marasca di verona' and 'spanska'. bud break and shooting were delayed and the developing leaves remained small. during the third year many kwanzan plants died, regardless of the indexed cultivar. electrophoretic analysis showed the presence of dsrna pattern in extracts of stunted kwanzan with a similar size to that of v ...200919463722
isolation of flavonoids from the heartwood and resin of prunus avium and some preliminary biological investigations.an investigation of the constituents in heartwood and resin of prunus avium is reported. a mini-library of structurally diverse flavanones and flavones was screened for human cytochrome p450 1a1, 3a4 and 19 (aromatase) inhibition, and for antifungal activity against a panel of pathogenic fungi. the defensive role of these natural plant flavonoids as antifungal phytoalexins and phytoanticipins is discussed.200919837443
bird diversity and seed dispersal along a human land-use gradient: high seed removal in structurally simple farmland.only few studies have analysed the relationship between biodiversity and ecosystem function at the landscape scale although relationships and mechanisms known from experimental studies might be different in natural systems. we quantified bird diversity and seed removal from 38 wild cherry trees (prunus avium) along a human land-use gradient from forest to structurally diverse to simple agricultural systems. high human land-use intensity led to low species richness and total abundance of the loca ...201020049479
acute toxicity effects of prunus avium fruit extract and selection of optimum dose against radiation exposure.the objective of the study was to evaluate the acute toxicity of different doses of the methanolic extract of the fruit pulp of prunus avium (family rosaceae), which is used ethno-medicinally for the treatment of various diseases, and to find out the optimal dose of prunus avium extract against 10 gy gamma-radiation exposure. to test acute toxicity in mice, different doses of pae (prunus avium fruit extract) were given orally for 15 consecutive days, after which the animals were observed for ano ...200920102327
reduced-risk management of rhagoletis cerasi flies (host race prunus) in combination with a preliminary phenological model.seasonal flight activity of rhagoletis cerasi (l.) (diptera: tephritidae) adults was monitored using yellow sticky traps at sweet cherry orchards under different management regimes in bursa, northwestern turkey, during 1997-1998. in the reduced-risk backyard orchards, soil ploughing in the fall or spring to destroy the pupae was combined with a single application of an insecticide, while conventionally managed orchards received six to seven insecticide applications for controlling adults. traps ...200620298111
fruits and vegetables protect against the genotoxicity of heterocyclic aromatic amines activated by human xenobiotic-metabolizing enzymes expressed in immortal mammalian cells.heterocyclic aromatic amines (haas) can be formed during the cooking of meat and fish at elevated temperatures and are associated with an increased risk for cancer. on the other hand, epidemiological findings suggest that foods rich in fruits and vegetables can protect against cancer. in the present study three teas, two wines, and the juices of 15 fruits and 11 vegetables were investigated for their protective effect against the genotoxic effects of 2-amino-3-methylimidazo[4,5-f]quinoline (iq) ...201020713174
determination of self-incompatibility groups of sweet cherry genotypes from turkey.determination of s-allele combinations of sweet cherry genotypes and cultivars has importance for both growers and breeders. we determined s-allele combinations of 40 local turkish sweet cherry genotypes using a pcr-based method. ten different s-alleles were detected. although the most common s-allele was s3, as also found in western genotypes and cultivars, there were some differences in the frequencies of some s-alleles between turkish and western sweet cherry genotypes. according to their s-a ...201121341217
trans-specific s-rnase and sfb alleles in prunus self-incompatibility haplotypes.self-incompatibility in the genus prunus is controlled by two genes at the s-locus, s-rnase and sfb. both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. deduced polypeptide sequences of three myrobalan and three domestic plum s-rnases showed over 97% identity with s-rnases from other prunus species, including almond, sweet cherry, japanese apricot and japanese plum. the second intron, which is generally highly polymorphic bet ...200817989997
functional characterization of palax1, a putative auxin permease, in heterologous plant systems.we have isolated the cdna of the gene palax1 from a wild cherry tree (prunus avium). the gene and its product are highly similar in sequences to both the cdnas and the corresponding protein products of aux/lax-type genes, coding for putative auxin influx carriers. we have prepared and characterized transformed nicotiana tabacum and arabidopsis thaliana plants carrying the gene palax1. we have proved that constitutive overexpression of palax1 is accompanied by changes in the content and distribut ...200818184737
radioprotection of swiss albino mice by prunus avium with special reference to hematopoietic system.prunus avium (family rosaceae) has been used ethnomedicinally for the treatment of many diseases, but its radioprotective efficacy has hardly been explored. presence of high anthocyanin content and phenolic compound with good antioxidative capacity has been reported by researchers. its radioprotective effect against 5, 7, 10 and 12 gy gamma radiation was evaluated by 30 day survival assay. regression analysis yielded ld50/30 5.81 and 9.43gy for irradiated only and pae + radiation groups, respect ...201121609316
influence of habitat complexity and landscape configuration on pollination and seed-dispersal interactions of wild cherry trees.land-use intensification is a major cause for the decline in species diversity in human-modified landscapes. the loss of functionally important species can reduce a variety of ecosystem functions, such as pollination and seed dispersal, but the intricate relationships between land-use intensity, biodiversity and ecosystem functioning are still contentious. along a gradient from forest to intensively used farmland, we quantified bee species richness, visitation rates of bees and pollination succe ...201121818655
genetic diversity in wild sweet cherries (prunus avium) in turkey revealed by ssr markers.wild sweet cherry (prunus avium) trees are abundant in the northern part of turkey, including the coruh valley. we analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper coruh valley in turkey to determine genetic variation, using 10 ssr primers. these ssr primers generated 46 alleles; the number of alleles per primer ranged from 3 to 7, with a mean of 4.6. the primer ps12a02 gave the highest number of polymorphic bands (n = 7), while cpsct010, udap-401 and udap ...201121732285
rapid gene-based snp and haplotype marker development in non-model eukaryotes using 3'utr sequencing.abstract: background: sweet cherry (prunus avium l.), a non-model crop with narrow genetic diversity, is an important member of sub-family amygdoloideae within rosaceae. compared to other important members like peach and apple, sweet cherry lacks in genetic and genomic information, impeding understanding of important biological processes and development of efficient breeding approaches. availability of single nucleotide polymorphism (snp)-based molecular markers can greatly benefit breeding eff ...201222239826
effect of several different pollens on the bio-ecological parameters of the predatory mite typhlodromus athenas swirski and ragusa (acari: phytoseiidae).the development, survivorship, and reproduction of the predacious mite typhlodromus athenas swirski and ragusa were studied in the laboratory by rearing the predator on nine different plant pollens [almond(prunus amygdalis batsch), apple (malus domestica borkh.), apricot (prunus armeniaca l.), cherry (prunus avium l.), pear (pyrus communis l.), plum (prunus domestica l.), walnut (juglans regia l.), olive (olea europaea l.), typha sp.], and pollen collected from bee hives. all experiments were c ...201122251637
fruit quality and bioactive compounds relevant to human health of sweet cherry (prunus avium l.) cultivars grown in italy.the fruit quality characteristics, phenolic compounds and antioxidant capacities of 24 sweet cherry (prunus avium l.) cultivars grown on the mountainsides of the etna volcano (sicily, italy) were evaluated. high-performance liquid chromatographic methods were used to identify and quantify sugars, organic acids and phenolics. a total of seven phenolic compounds were characterised as hydroxycinnamic acid derivatives (neochlorogenic acid, p-coumaroylquinic acid and chlorogenic acid) and anthocyanin ...201223692746
association between chloroplast and mitochondrial dna sequences in chinese prunus genotypes (prunus persica, prunus domestica, and prunus avium).the nuclear dna is conventionally used to assess the diversity and relatedness among different species, but variations at the dna genome level has also been used to study the relationship among different organisms. in most species, mitochondrial and chloroplast genomes are inherited maternally; therefore it is anticipated that organelle dna remains completely associated. many research studies were conducted simultaneously on organelle genome. the objectives of this study was to analyze the genet ...201525592231
cherry.agrobacterium tumefaciens-mediated transformation of sour chgahtvy (prunus cerasus l.) "montmorency" and sweet cherry rootstocks "gisela 6" and "gisela 7" (p. cerasus × p. canescens) is described. briefly, leaf explants from in vitro shoots are cocultivated with a. tumefaciens either directly (for "gisela 6" and "gisela 7") or after pretreatment (for "montmorency") on cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/l kan ...201525416255
rhizobium nepotum sp. nov. isolated from tumors on different plant species.five gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on different plant species in hungary: strain 39/7(t) from prunus cerasifera myrobalan, strain 0 from grapevine var. ezerjó, strain 7/1 from raspberry var. findus and in poland, strain c3.4.1 from colt rootstock (prunus avium × prunus pseudocerasus) and strain cp17.2.2 from prunus avium. only one of these isolates, strain 0, is able to cause crown gall on different plant species. on the basis of 16s rrna gene seq ...201222463808
antifungal effect of volatile organic compounds produced by bacillus amyloliquefaciens cpa-8 against fruit pathogen decays of cherry.the present work focuses on the antifungal effect of volatile organic compounds (vocs) produced by bacillus amyloliquefaciens cpa-8 against monilinia laxa, m. fructicola and botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. vocs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. for this purpose, cpa-8 was grown on different media and cultured for 24 and 48 h at 30 °c before assays. data showed that mycelial growth inhibi ...201728213029
measurement of antioxidant activity and antioxidant compounds under versatile extraction conditions: i. the immuno-biochemical antioxidant properties of sweet cherry (prunus avium) extracts.previously, we have meticulously examined the efficacy of the measurable antimicrobial activity of sweet cherry (prunus avium) extracts on a wide spectrum of gram-positive and gram-negative bacteria, in addition to the fungus, candida albicans, a priori. in order to further understand the biochemical constituents and antioxidant activities of a variety of extracts of sweet cherries, antioxidant compounds of immunological significance, including l-ascorbic acid (vitamin c), phenols, flavonoids, a ...201323531198
survey of cherry necrotic rusty mottle virus and cherry green ring mottle virus incidence in korea by duplex rt-pcr.the incidence of cherry necrotic rusty mottle virus (cnrmv) and cherry green ring mottle virus (cgrmv) have recently been occurred in korea, posing a problem for sweet cherry cultivation. since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. in this study, the incidence of cnrmv and cgrmv in sweet cherry in gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (rt-pc ...201425506311
genomic analyses of cherry rusty mottle group and cherry twisted leaf-associated viruses reveal a possible new genus within the family betaflexiviridae.it is demonstrated that closely related viruses within the family betaflexiviridae are associated with a number of diseases that affect sweet cherry (prunus avium) and other prunus spp. cherry rusty mottle-associated virus (crmav) is correlated with the appearance of cherry rusty mottle disease (crmd), and cherry twisted leaf-associated virus (ctlav) is linked to cherry twisted leaf disease (ctld) and apricot ringpox disease (arpd). comprehensive analysis of previously reported full genomic sequ ...201525496302
viruses associated with rusty mottle and twisted leaf diseases of sweet cherry are distinct species.virus rna sequences related to those of the family betaflexiviridae were amplified from trees affected with the following diseases: cherry twisted leaf, apricot ring pox, cherry necrotic rusty mottle, cherry rusty mottle, and cherry green ring mottle. phylogenetic analysis of virus sequences obtained from these diseased trees from western north america, along with published sequences of cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv), revealed four major cla ...201324219146
complete nucleotide sequence of a virus associated with rusty mottle disease of sweet cherry (prunus avium).cherry rusty mottle is a disease of sweet cherries first described in 1940 in western north america. because of the graft-transmissible nature of the disease, a viral nature of the disease was assumed. here, the complete genomic nucleotide sequences of virus isolates from two trees expressing cherry rusty mottle disease symptoms are characterized; the virus is designated cherry rusty mottle associated virus (crmav). the biological and molecular characteristics of this virus in comparison to thos ...201323525699
complete nucleotide sequences and genome organization of a cherry isolate of cherry leaf roll virus.the complete nucleotide sequence of cherry leaf roll virus (clrv, genus nepovirus) from a naturally infected cherry tree (prunus avium cv. bing) in north america was determined. rna1 and rna2 consist of 7,893 and 6,492 nucleotides, respectively, plus a poly-(a) tail. each rna encodes a single potential open reading frame. the first 657 nucleotides of rna1 and rna2 are 99% identical and include the 5'-utr and the first 214 deduced amino acids of the polyproteins following the first of two in-fram ...201222218961
simultaneous detection and identification of four cherry viruses by two step multiplex rt-pcr with an internal control of plant nad5 mrna.a multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed and standardized for the simultaneous detection of four cherry viruses: cherry virus a (cva, genus; capillovirus), cherry necrotic rusty mottle virus (cnrmv, unassigned species of the betaflexiviridae), little cherry virus 1 (lchv-1, genus; closterovirus) and prunus necrotic ringspot virus (pnrsv, genus; ilarvirus) with nad5 as plant internal control. a reliable and quick method for total plant rna extraction fro ...201323707922
complete nucleotide sequence of cherry virus a (cva) infecting sweet cherry in india.cherry virus a (cva) is a graft-transmissible member of the genus capillovirus that infects different stone fruits. sweet cherry (prunus avium l; family rosaceae) is an important deciduous temperate fruit crop in the western himalayan region of india. in order to determine the health status of cherry plantations and the incidence of the virus in india, cherry orchards in the states of jammu and kashmir (j&k) and himachal pradesh (h.p.) were surveyed during the months of may and september 2009. t ...201020938696
evaluation of the allergenicity potential of tcpr-10 protein from theobroma cacao.the pathogenesis related protein pr10 (tcpr-10), obtained from the theobroma cacao-moniliophthora perniciosa interaction library, presents antifungal activity against m. perniciosa and acts in vitro as a ribonuclease. however, despite its biotechnological potential, the tcpr-10 has the p-loop motif similar to those of some allergenic proteins such as bet v 1 (betula verrucosa) and pru av 1 (prunus avium). the insertion of mutations in this motif can produce proteins with reduced allergenic power ...201222768037
effect of phytosanitary irradiation and methyl bromide fumigation on the physical, sensory, and microbiological quality of blueberries and sweet cherries.the objective of this study was to determine whether irradiation could serve as a suitable phytosanitary treatment alternative to methyl bromide (mb) fumigation for blueberries and sweet cherry and also to determine the effect of phytosanitary irradiation treatment on survival of salmonella spp. and listeria monocytogenes on these fruit. 'bluecrop' blueberries (vaccinium corymbosum) and 'sweetheart' cherries (prunus avium) were irradiated at 0.4 kgy or fumigated with methyl bromide and evaluated ...201626805024
rapid and sensitive detection of little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification.little cherry virus 2 (lchv2) (genus ampelovirus) is the primary causal agent of little cherry disease (lcd) in sweet cherry (prunus avium) in north america and other parts of the world. this mealybug-transmitted virus does not induce significant foliar symptoms in most sweet cherry cultivars, but does cause virus-infected trees to yield unevenly ripened small fruits with poor flavor. most fruits from infected trees are unmarketable. in the present study, an isothermal reverse transcription-reco ...201424797461
mortality and oviposition of western cherry fruit fly (diptera: tephritidae) exposed to different insecticide baits for varying periods in the presence and absence of food.spinosad bait is used to control western cherry fruit fly, rhagoletis indifferens curran (diptera: tephritidae), by killing flies before they oviposit. however, effects of different insecticide baits on management of reproductively mature flies are largely unknown. objectives here were to determine mortality and oviposition of reproductively mature r. indifferens exposed to different insecticide baits for varying periods in the presence and absence of dried yeast extract and sucrose food. spinos ...201121404858
honeybees increase fruit set in native plant species important for wildlife conservation.honeybee colonies are declining in some parts of the world. this may have important consequences for the pollination of crops and native plant species. in spain, as in other parts of europe, land abandonment has led to a decrease in the number of non professional beekeepers, which aggravates the problem of honeybee decline as a result of bee diseases in this study, we investigated the effects of honeybees on the pollination of three native plant species in northern spain, namely wildcherry prunu ...201121519875
genetic diversity in the coat protein genes of prune dwarf virus isolates from sweet cherry growing in turkey.sweet cherry is an important fruit crop with increasing economical value in turkey and the world. a number of viruses cause diseases and economical losses in sweet cherry. prune dwarf virus (pdv), is one of the most common viruses of stone fruits including sweet cherry in the world. in this study, pdv was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of isparta province of turkey by double antibody sandwich-enzyme linked immunosorbent assay (das-elisa) ...201525774109
engineering cherry rootstocks with resistance to prunus necrotic ring spot virus through rnai-mediated silencing.prunus necrotic ringspot virus (pnrsv) is a major pollen-disseminated ilarvirus that adversely affects many prunus species. in this study, an rna interference (rnai) vector part27-pnrsv containing an inverted repeat (ir) region of pnrsv was transformed into two hybrid (triploid) cherry rootstocks, 'gisela 6' (gi 148-1) and 'gisela 7'(gi 148-8)', which are tolerant and sensitive, respectively, to pnrsv infection. one year after inoculation with pnrsv plus prune dwarf virus, nontransgenic 'gisela ...201323521804
high-throughput sequencing as an effective approach in profiling small rnas derived from a hairpin rna expression vector in woody plants.hairpin rna (hprna)-mediated gene silencing has proved to be an efficient approach to develop virus-resistant transgenic plants. to characterize small rna molecules (srnas) derived from an hprna expression vector in transgenic cherry rootstock plants, we conducted small rna sequencing of (1) a transgenic rootstock containing an inverted repeat of the partial coat protein of prunus necrotic ring spot virus (pnrsv-hprna); (2) a nontransgenic rootstock; and (3) a pnrsv-infected sweet cherry plant. ...201425438784
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